摘要：
Recycling livestock manure in agroecosystems can maintain crop production, improve soil fertility, and reduce environmental losses. However, there has been no comprehensive assessment of synergies and trade-offs in the food-energy-soil-environment nexus under manure application. Here, we evaluate the sustainability of maize production under four fertilization regimes (mineral, mineral and manure mixed, manure, and no fertilization) from the aspect of food security, energy output, soil quality, and environmental impact based on a five-year field experiment. Manure and mineral mixed fertilization maintained grain and straw quantity and quality compared with mineral fertilization. Manure and mineral mixed fertilization increased stem/leaf ratio and field residue index by 9.1-28.9% and 4.5-17.9%, respectively. Manure also maintained the theoretical ethanol yield but reduced the straw biomass quality index by increasing ash. Further, manure application increased the soil quality index by 40.5% and reduced N(2)O emissions by 55.0% compared with mineral fertilization. Manure application showed the highest sustainability performance index of 19, followed by mineral (15), mixed (13), and without fertilization (8). In conclusion, manure application maintains food production and energy output, enhances soil quality, and reduces environmental impact, thereby improving the sustainability of maize production.
摘要：
Simple Summary: The intestinal health problems of weaned piglets cause serious economic losses. Historically, antibiotics have been used to prevent and treat intestinal problems in weaned piglets. However, the prohibition of antibiotics makes us need to find a safer and effective solution strategy. A large number of studies have shown that the endocannabinoid system, as a lipid mediator signaling system widely distributed in the gastrointestinal tract, interacts with the intestinal microbiota and maintains intestinal homeostasis. In this study, we found that the endocannabinoid system is closely related to the abundance of Lactobacillus johnsonii through a piglet antibiotic model. It was further found that Lactobacillus johnsonii could improve intestinal health problems and alleviate piglet diarrhea. At the same time, its ability to regulate the endocannabinoid system was verified, and the correlation analysis found that its benefits are partly achieved through the participation of the endocannabinoid system. Probiotic intervention is a well-established approach for replacing antibiotics in the management of weaning piglet diarrhea, which involves a large number of complex systems interacting with the gut microbiota, including the endocannabinoid system; nevertheless, the specific role of the endocannabinoid system mediated by probiotics in the piglet intestine has rarely been studied. In this study, we used antibiotics (ampicillin) to perturb the intestinal microbiota of piglets. This resulted in that the gene expression of the intestinal endocannabinoid system was reprogrammed and the abundance of probiotic Lactobacillus johnsonii in the colon was lowered. Moreover, the abundance of Lactobacillus johnsonii was positively correlated with colonic endocannabinoid system components (chiefly diacylglycerol lipase beta) via correlation analysis. Subsequently, we administered another batch of piglets with Lactobacillus johnsonii. Interestingly, dietary Lactobacillus johnsonii effectively alleviated the diarrhea ratio in weaning piglets, accompanied by improvements in intestinal development and motility. Notably, Lactobacillus johnsonii administration enhanced the intestinal barrier function of piglets as evidenced by a higher expression of tight junction protein ZO-1, which might be associated with the increased level in colonic diacylglycerol lipase beta. Taken together, the dietary Lactobacillus johnsonii-mediated reprogramming of the endocannabinoid system might function as a promising target for improving the intestinal health of piglets.
关键词：
Pullulan;Starch nanoplatelets;Vein-like structure;Mechanical performance;Graph theory
摘要：
Inspired by the leaf-vein network structure, the pullulan-starch nanoplatelets (SNPs) bioinspired films with enhanced strength and toughness were successfully fabricated through a water evaporation-induced self -assembly technique. SNPs (SNP200 and SNP600) of two sizes were separated by differential centrifugation. Interactions between SNPs and pullulan during drying resulted in the vein-like network structure in both nanocomposite films when the appropriate amounts of SNP200 or SNP600 were added to pullulan, respectively. The TS and toughness values of pullulan with 1 % w/w SNP200 films reached up to 51.05 MPa and 69.65 MJ.m(-3), which were 86 % and 223 % higher than those of the neat pullulan films, respectively. Moreover, the TS and toughness values of pullulan-SNP200 were significantly higher than those of pullulan-SNP600 films, when SNP content exceeded the 1 % w/w level. By applying a graph theory, the network structures were found to correlate with the mechanical properties of the pullulan-SNPs bioinspired films. The new strategy for designing starch nanoplatelets-based edible films that combine mechanical strength and toughness holds promises for the development of novel biobased composite materials for food packaging application.
摘要：
Cowpea (Vigna unguiculata L.) is a legume consumed as a high-quality plant protein source in many parts of the world. In August 2023, it was observed that a plant disease affected cowpea growth in Yiyang (28.34°N, 112.55°E), China. The average disease incidence was 10%, resulting in 8.5% economic losses in approximately 3,000 m(2). The symptoms initially appeared as brown lesions near the stem-soil interface and the lesions were colonized by white mycelia. As the disease progressed, the disease symptoms included constriction and brown staining at the base of the stem, covered with a small amount of white mycelia. Eventually, the entire plants withered and collapsed and many sclerotia were scattered on the ground around the diseased stem. Twenty samples (10 sclerotia and 10 diseased tissue fragments) were collected from symptomatic plants for causal agent isolation. Samples were disinfected with 70% ethanol for 30 s, 5% NaClO for 1 min, rinsed three times with sterile water, dried and placed on potato dextrose agar (PDA) plates at 28℃ in the dark. In total, 20 isolates were obtained by the hyphal tip method (Terrones et al. 2022) and showed a consistent phenotype of white cottony mycelia on PDA with an growth rate of 12.9 to 21.3 mm/day (n = 20). Sclerotia formed at five to eight days post inoculation, were initially whitish, turning beige and eventually dark brown. The diameter of mature sclerotia ranged from 0.89 to 2.13 mm (mean = 1.64±0.29 mm; n =50). For pathogen identification, ITS1/ITS4 (White et al. 1990) and EF1-983F/EF1-2218R (Rehner and Buckley 2005) primers were used to amplify the internal transcribed spacer regions (ITS) and translation elongation factor-1 alpha gene (TEF-1α), respectively. The sequences of all 20 isolates showed 99% to 100% similarity withAgroathelia rolfsii sequences from GenBank by BLAST analysis. The sequences of two representative strains, ID1 and ID4, were deposited in GenBank. The ITS sequences of ID1 (OR689482) and ID4 (OR689481) were ＞99% similar to A. rolfsii strain QJ7 (593/596 bp; MZ750983) and A. rolfsii strain Kale078 (565/568 bp; MN872304), respectively. Also, TEF-1α sequences of ID1 (OR713735) and ID4 (OR713736) were ＞99% similar to the sequences of A. rolfsii strain HS-Sr (1073/1073 bp; OL416131) and A. rolfsii strain MSB1-2 (1070/1075 bp; MN702790), respectively. Phylogenetic analysis based on ITS and TEF1-α sequences indicated that ID1 and ID4 clustered into the A. rolfsii clade.Based on morphology and sequence analyses, the isolates ID1 and ID4 were identified as A. rolfsii (anamorph Sclerotium rolfsii).Pathogenicity tests were conducted three times on healthy 30-day-old cowpea seedlings. Five plants were inoculated with 6-day-old mycelial discs (6 mm) of ID1 or ID4 at the base of the seedlings (n = 30) while four plants were inoculated with a sterile PDA disc as a control (n = 12). All seedlings were cultivated in a greenhouse with a temperature of 26°C to 28°C and relative humidity 60% to 80% with a 14/10 h light/dark photoperiod. Eight days later, all the fungal inoculated seedlings showed symptoms including brown necrosis and collapse of the stems, and eventual withering of the seedlings. Control plants remained asymptomatic. The causal pathogens were reisolated in PDA plates and identified by ITS sequence analysis, completing Koch's postulates. To our knowledge, this is the first report ofA. rolfsiicausing southern blight oncowpea in China. Early accurate diagnosis will help farmers to adopt suitable practices to control disease outbreaks and reduce losses.
期刊：
Environment, Development and Sustainability,2024年 ISSN：1387-585X
通讯作者：
Chen, JY
作者机构：
[Dong, Xuesong] Hunan Agr Univ, Sch Business, Changsha 410083, Peoples R China.;[Chen, Jinyu; Huang, Jianbai] Cent South Univ, Sch Business, Changsha 410083, Peoples R China.;[Chen, Jinyu; Dong, Xuesong; Huang, Jianbai] Cent South Univ, Inst Met Resources Strategy, Changsha 410083, Peoples R China.
通讯机构：
[Chen, JY ] C;Cent South Univ, Sch Business, Changsha 410083, Peoples R China.;Cent South Univ, Inst Met Resources Strategy, Changsha 410083, Peoples R China.
关键词：
Metal consumption;Input-output analysis;Structural path analysis;Structural decomposition analysis
摘要：
The dependence of economic development on the metal resource is one of the challenges for developing countries. Given the enormous pressure of resource shortage in China, exploring the flow of metals between sectors is critical to achieve the Sustainable Development Goals. Based on the Chinese environmentally extended input-output (CEEIO) database from 1997 to 2017, the structural decomposition analysis (SDA) and the structural path analysis (SPA) models were combined to analyze the main driving factors and key paths of metal consumption in China. The results show that from 1997 to 2017, China's economic development and metal consumption presented states of weak decoupling and expansion coupling. Capital formation, consumption expansion, and export expansion were main drivers of metal consumption. Sectors such as metal smelting, construction, and other manufacturing products consumed a large amount of metal directly or provide intermediate products for other sectors. In the future, the upstream and downstream links of the industrial chain should be managed according to the key path, so as to optimize the structure of intermediate products and final demand and realize metal resources conservation from production to consumption.
摘要：
The phenylalanine ammonia-lyase (PAL) enzyme catalyses the conversion of l-phenylalanine to trans-cinnamic acid. This conversion is the first step in phenylpropanoid biosynthesis in plants. The phenylpropanoid pathway produces diverse plant metabolites that play essential roles in various processes, including structural support and defence. Previous studies have shown that mutation of the PAL genes enhances disease susceptibility. Here, we investigated the functions of the rice PAL genes using 2-aminoindan-2-phosphonic acid (AIP), a strong competitive inhibitor of PAL enzymes. We show that the application of AIP can significantly reduce the PAL activity of rice crude protein extracts in vitro. However, when AIP was applied to intact rice plants, it reduced infection of the root-knot nematode Meloidogyne graminicola. RNA-seq showed that AIP treatment resulted in a rapid but transient upregulation of defence-related genes in roots. Moreover, targeted metabolomics demonstrated higher levels of jasmonates and antimicrobial flavonoids and diterpenoids accumulating after AIP treatment. Furthermore, chemical inhibition of the jasmonate pathway abolished the effect of AIP on nematode infection. Our results show that disturbance of the phenylpropanoid pathway by the PAL inhibitor AIP induces defence in rice against M. graminicola by activating jasmonate-mediated defence.
关键词：
Slow and controlled -release fertilisers;Screw fertiliser distributor;Granular fertiliser model;Fragmentation;Hertz-mindlin with bonding
摘要：
The investigation of the fragmentation law of granular fertilisers in side-deep fertilisation devices is crucial for optimizing machinery. In this paper, Hertz-Mindlin with Bonding was used to establish a granular fertiliser model with breakage properties. The single-factor and central composite tests were carried out with the screw pitch, screw speed, and Y-shaped mouth angle selected as the test factors, and the broken bond rate as the evaluation index to indicate the fragmentation rate of the granular fertilisers. The result indicated a positive correlation between broken bond rate and screw speed in the range of 100-300 rpm, as well as a negative correlation between broken bond rate and screw pitch in the range of 18-38 mm and the Y-shaped mouth angle in the range of 25-45(degrees). Moreover, during the reduction in screw pitch from 33 to 23 mm, the increment of broken bond rate caused by screw pitch was increased due to the higher screw speed and decreased due to the larger Yshaped mouth angle. The key factors, in order of importance, are the screw pitch, screw speed, and Y-shaped mouth angle. In addition, the verification test was carried out in the range of 100-300 rpm and the test results showed a similar response trend to the simulation results, indicating that the fragmentation law obtained by the simulation is reliable. These research findings can provide a reference for the optimization of the side-deep fertilisation devices.
摘要：
Tobacco (Nicotiana tabacum L.) is an important economic crop that is widely grown around the world. Its annual production in China is estimated at 2.2 million tons (Berbeć and Matyka 2020). Since 2022, a root rot disease was sporadically observed on tobacco seedlings on cultivar Yunyan 87 in cultivated tobacco fields in the Hunan province of China. A disease incidence of about 10% occurred across 48 ha of tobacco fields. The affected tobacco plants had slow and stunted growth with yellowing leaves. The roots turned grayish brown, decayed, and died. Diseased roots were collected from six fields and cut into small pieces (5 mm ×5 mm) from the edge of the rotted portions, and then sterilized with 70% ethanol for 10 s, 0.1% HgCl2 for 1 min, and washed in sterilized water three times. All the sterilized tissue were placed on potato dextrose agar (PDA) medium and cultured at 26 ℃ in the dark. About 3 days later, colonies with similar morphology were removed and sub-cultured on fresh PDA. A total of six strains were obtained from six tobacco samples. Strains were white and had radial growth on PDA. Hyphae were aseptate and the sporangia were filamentous. The oogonia were subglobose, smooth, 16.04 ± 0.25 µm (n=50) in diameter, and developed on unbranched stalks. The antheridia were barrel shaped and clavate. Oospores were globose, aplerotic or nearly plerotic, measuring 6.62 ± 0.33 µm (n=50). These morphological characteristics were consistent with the description of Pythium spp. (van der Plaats-Niterink 1981). For molecular identification, the internal transcribed spacer (ITS) region of rDNA and cytochrome c oxidase subunit I (Cox I) of a representative isolate, GF-3, were amplified and sequenced (GenBank accession nos. OR228424 for ITS and OR237556 for Cox I) using universal primers ITS1/ITS4 (White et al. 1990) and FM58/FM66, respectively (Villa et al. 2006). BLASTn analysis revealed that the ITS and Cox I sequences were 99.76 % (838/840 bp) and 99.85% (671/672 bp) identical to the corresponding sequences of P. dissotocum strain CBS 166.68 (AY598634.2) and UM982 (MT981147.1), respectively. A neighbor-joining phylogenetic tree based on the Cox I sequence showed that GF-3 grouped in the P. dissotocum branch. Based on morphological and molecular characteristics, GF-3 was identified to be P. dissotocum. For pathogenicity testing, four- to five-leaf-old healthy potted tobacco seedlings of the Yunyan 87 cultivar were inoculated with a zoospore suspension (1 × 105 zoospores/ml), which was induced on V8-juice medium. The zoospore suspension was introduced into the soil around plant roots and 10 mL of inoculum was used for each plant. In the control group, plants were inoculated with sterilized water. All of the treated plants were kept in humid chambers at 26°C under a 12 h/12 h photoperiod. The pathogenicity assays were performed twice, with each treatment having three replicated plants. After 5 days, tobacco seedlings inoculated with P. dissotocum showed symptoms resembling that observed in the field. However, the control plants remained healthy. Pythium dissotocum was re-isolated from the infected plants and identified by morphological and molecular methods, thus confirming Koch's postulates. Pythium dissotocum has been reported causing root rot in other plants, including hydroponic lettuce (McGehee et al. 2018) and spinach (Huo et al. 2020). Also, many Pythium species have recently been recovered from float-bed tobacco transplant production greenhouses (Zhang et al. 2022). However, to our knowledge, this is the first report of root rot on tobacco caused by P. dissotocum in China. Since this disease could greatly affect tobacco seedling establishment in the field, appropriate management strategies need to be developed to reduce further losses in tobacco planting fields.
摘要：
This study addresses the challenge of visual localization using monocular images, a crucial technology for autonomous systems that facilitates their navigation and interaction capabilities. With the advent of deep learning, visual localization techniques that utilize these methods have demonstrated improved robustness across diverse environments. Existing end-to-end models apply convolutional neural networks (CNNs) to extract salient features and directly estimate continuous spatial poses from map models that allow for implicit differentiation. Nonetheless, these models often falter in adapting their feature representations to extreme variations in environmental conditions, leading to critical localization inaccuracies during episodes of altered lighting, varying weather, or in the presence of moving objects. To overcome these limitations, we introduce the end-to-end feature refinement network for visual localization (EFRNet-VL). This network architecture is specifically designed to prioritize the extraction of static features crucial for the six degrees of freedom (6DoF) pose estimation, thereby outperforming prior methodologies. EFRNet-VL meticulously integrates a convolutional network structure with self-attention mechanisms and Long Short-Term Memory (LSTM) modules, which together facilitate the accurate association of a single image with its corresponding camera pose, even within dynamic environments. The proposed feature refinement approach is straightforward to implement and can enhance the performance of existing neural pose estimators. Our comprehensive evaluations of EFRNet-VL underscore its effectiveness. Notably, it has diminished the average position and orientation errors by 54.5% and 25.7%, respectively, as compared to the popular PoseNet model across various indoor settings. Moreover, in large-scale outdoor environments, it has achieved an average localization precision of 7.02m/2.79 degrees. EFRNet-VL has set a new benchmark for end-to-end learning-based methods in visual localization and operates efficiently in real time, processing at a speed of 9.8 ms per image frame.
摘要：
Due to the low storage cost and high computation efficiency of hashing, cross-modal hashing has been attracting widespread attention in recent years. In this paper, we investigate how supervised cross-modal hashing (CMH) benefits from multi-label and contrastive learning (CL) by overcoming the following two challenges: (i) how to combine multi-label and supervised contrastive learning to consider diverse relationships among cross-modal instances, and (ii) how to reduce the sparsity of multi-label representation so as to improve the similarity measurement accuracy. To this end, we propose a novel cross-modal hashing framework, dubbed Multi-Label Weighted Contrastive Hashing (MLWCH). This framework involves compact consistent similarity representation, a new designed multi-label similarity calculation method that efficiently reduces the sparsity of multi-label by reducing redundant zero elements. Furthermore, a novel multi-label weighted contrastive learning strategy is developed to significantly improve hashing learning by assigning similarity weight to positive samples under both linear and non-linear similarities. Extensive experiments and ablation analysis over three benchmark datasets validate the superiority of our MLWCH method, especially over several outstanding baselines.
摘要：
We introduce a localized version of a Galambos's question about the growth speed of the digits in Engel expansion, namely the set{ 1 } x & ISIN; (0, 1] : lim nlog dn(x) = & alpha;(x) , n & RARR;& INFIN;where & alpha; : [0, 1] & RARR; [0, & INFIN;] is a nonnegative continuous function and dn(x) denotes the nth digits in the Engel expansion of x. The Hausdorff dimension is shown to be irrelevant of the function & alpha;(x). As applications, this answers Galambos's question and strengthens some results by Liu & Wu.& COPY; 2023 Elsevier Inc. All rights reserved.
关键词：
Straw application;Greenhouse gas emission;Functional genes;Carbon footprint;Net ecological economic benefits
摘要：
With the increasing application of crop straw, the greenhouse gas emissions caused due to crop straw have received increasing attention. However, the current studies have not explored the greenhouse gas emissions from on-farmland consumption of returned straw. Based on a long-term positioning experiment, three treatments were set up in double-cropping rice fields: chemical fertilizer (CF), straw replacing 1/3 nitrogen fertilizer (MS), and straw replacing 2/3 nitrogen fertilizer (HS). We measured the biomass, soil properties, and greenhouse gas emissions of double-cropping rice and then comprehensively evaluated the global warming potential, carbon footprint, and net ecological and economic benefits. The straw treatments (MS and HS) increased the cumulative annual emissions of CH4 (98.44% and 261.23%), CO2 (30.85% and 122.29%), and N2O (7.37% and 52.50%), the cumulative annual global warming potential (74.15% and 206.12%), average GHG intensity (43.26% and 138.07%), and the annual cumulative net ecosystem carbon budget (52.96% and 100.97%) in the early and late rice growing seasons, respectively. We observed that the real-time greenhouse gas emissions were significantly correlated to the soil microbial functional genes and total carbon, NH4+-N, and NO3--N. The results of the random forest model showed that total carbon (13.87%) and nirK abundance (9.80%) were the highest predictors of global warming potential at the booting and maturity stages, respectively during rice growing season. Combining the resource inputs for agricultural production and the greenhouse gas emission potential of the returned straw on its own, MS showed the lowest net greenhouse gas emission, and MS and HS showed significantly increased annual cumulative net ecological economic benefits (38.08% and 34.30%). Overall, MS showed the lowest net greenhouse gas emission and the highest net ecological economic benefits, which is a straw-returning measure with low environmental impact and high economic returns.
摘要：
Soil salinization is one of the most important abiotic stresses which can seriously affect the growth and development of rice, leading to the decrease in or even loss of a rice harvest. Increasing the rice yield of saline soil is a key issue for agricultural production. The utilization of heterosis could significantly increase crop biomass and yield, which might be an effective way to meet the demand for rice cultivation in saline soil. In this study, to elucidate the regulatory mechanisms of rice hybrids and their parents that respond to salt stress, we investigated the phenotypic characteristics, physiological and biochemical indexes, and expression level of salt-related genes at the seedling stage. In this study, two sets of materials, encapsulating the most significant differences between the rice hybrids and their parents, were screened using the salt damage index and a hybrid superiority analysis. Compared with their parents, the rice hybrids Guang-Ba-You-Hua-Zhan (BB1) and Y-Liang-You-900 (GD1) exhibited much better salt tolerance, including an increased fresh weight and higher survival rate, a better scavenging ability towards reactive oxygen species (ROS), better ionic homeostasis with lower content of Na+ in their Na+/K+ ratio, and a higher expression of salt-stress-responsive genes. These results indicated that rice hybrids developed complex regulatory mechanisms involving multiple pathways and genes to adapt to salt stress and provided a physiological basis for the utilization of heterosis for improving the yield of rice under salt stress.
作者机构：
[Wu, Xiongwei; Ling, Wei; Zeng, Xian-Xiang] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;[Huang, Y; Mo, Funian; Huang, Yan; Ling, Wei] Harbin Inst Technol, Sch Mat Sci & Engn, Sauvage Lab Smart Mat, Shenzhen 518055, Peoples R China.;[Luo, Guangfu; Lu, Zhouguang; Nie, Chenxi] Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China.;[Huang, Y; Huang, Yan; Ling, Wei] Harbin Inst Technol, State Key Lab Adv Welding & Joining, Shenzhen Key Lab Flexible Printed Elect Technol, Shenzhen 518055, Peoples R China.;[Ma, Qiang] Henan Univ Engn, Coll Mat Engn, Henan Int Joint Lab Rare Earth Composite Mat, Zhengzhou 451191, Peoples R China.
通讯机构：
[Luo, GF ] S;[Huang, Y ] H;Harbin Inst Technol, Sch Mat Sci & Engn, Sauvage Lab Smart Mat, Shenzhen 518055, Peoples R China.;Southern Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China.;Harbin Inst Technol, State Key Lab Adv Welding & Joining, Shenzhen Key Lab Flexible Printed Elect Technol, Shenzhen 518055, Peoples R China.
关键词：
high energy density;high zinc utilization;ion−sieve interface;mild aqueous zinc ion batteries;zinc anode
摘要：
The cycling stability of a thin zinc anode under high zinc utilization has a critical impact on the overall energy density and practical lifetime of zinc ion batteries. In this study, an ion sieve protection layer (ZnSnF@Zn) was constructed in situ on the surface of a zinc anode by chemical replacement. The ion sieve facilitated the transport and desolvation of zinc ions at the anode/electrolyte interface, reduced the zinc deposition overpotential, and inhibited side reactions. Under a 50% zinc utilization, the symmetrical battery with this protection layer maintained stable cycling for 250 h at 30 mA cm(-2). Matched with high-load self-supported vanadium-based cathodes (18-20 mg cm(-2)), the coin battery with 50% zinc utilization possessed an energy density retention of 94.3% after 1000 cycles at 20 mA cm(-2). Furthermore, the assembled pouch battery delivered a whole energy density of 61.3 Wh kg(-1), surpassing the highest mass energy density among reported mild zinc batteries, and retained 76.7% of the energy density and 85.3% (0.53 Ah) of the capacity after 300 cycles.
摘要：
Cadmium (Cd) is a highly toxic heavy metal that causes serious damage to plant and human health. Phytolacca acinosa Roxb. has a large amount of aboveground biomass and a rapid growth rate, and it has been identified as a novel type of Cd hyperaccumulator that can be harnessed for phytoremediation. However, the molecular mechanisms underlying the response of P. acinosa to Cd2+ stress remain largely unclear. In this study, the phenotype, biochemical, and physiological traits of P. acinosa seeds and seedlings were analyzed under different concentrations of Cd2+ treatments. The results showed higher Cd2+ tolerance of P. acinosa compared to common plants. Meanwhile, the Cd2+ content in shoots reached 449 mg/kg under 10 mg/L Cd2+ treatment, which was obviously higher than the threshold for Cd hyperaccumulators. To investigate the molecular mechanism underlying the adaptability of P. acinosa to Cd stress, RNA-Seq was used to examine transcriptional responses of P. acinosa to Cd stress. Transcriptome analysis found that 61 genes encoding TFs, 48 cell wall-related genes, 35 secondary metabolism-related genes, 133 membrane proteins and ion transporters, and 96 defense system-related genes were differentially expressed under Cd2+ stress, indicating that a series of genes were involved in Cd2+ stress, forming a complex signaling regulatory mechanism. These results provide new scientific evidence for elucidating the regulatory mechanisms of P. acinosa response to Cd2+ stress and new clues for the molecular breeding of heavy metal phytoremediation.
摘要：
Collision-free grasping of the thin, brief peduncles connecting cherry tomato clusters to the main stem was crucial for tomato harvesting robots. Recognizing that the optimal operating posture for each individual peduncle was various, this study proposed a novel peduncle grasping posture decision model using deep reinforcement learning (DRL) for tomato harvesting manipulators, to overcome the collision issue caused by fixed-posture grasping. This model could dynamically generated action sequences for the harvesting manipulator, ensuring that the end-effector approach to the peduncle along the collision-free path with the optimal grasping posture. Building upon prior research into the multi-task identification of tomato clusters, peduncles, and the main stem, a keypoint-based spatial pose description model for tomato bunches was devised. Through this, the optimal operating posture for the end-effector on the peduncle was established. An improved HER-SAC (Soft Actor Critic with Hindsight Experience Replay) algorithm was subsequently established to guide the end-effector in collision-free grasping motions. The reward function of this algorithm incorporated end-effector posture constraints obtained from the optimal posture plane. In the training phase, a heuristic strategy model, providing prior knowledge, was merged with a dynamic gain module to sidestep local optimal policies, collectively enhancing the learning efficiency. In the simulation, our method improved the success rate of the peduncle grasping by at least 14 %, compared with SAC, HER-DDPG and HER-TD3. For the identical scenarios, improved HER-SAC reached the desired posture with a minimum of 15.5 % fewer steps compared to other algorithms. In field experiments conducted in tomato greenhouses, the robot achieved a harvesting success rate of 85.5 %, which was an increase of 57.3 % and 43.0 % compared to traditional methods with fixed horizontal and parallel-to-main-stem postures, respectively. The average operation time, from identification to successful harvesting, was 11.42 s. Our findings offer a promising solution to enhancing the efficiency of tomato-harvesting robots.
关键词：
L. delbrueckii;ileal bacteria;circadian rhythms;pigs
摘要：
Intestinal bacteria, synchronized with diet and feeding time, exhibit circadian rhythms and anticipate host gut function; however the effect of dietary probiotics on gut bacterial diurnal rhythms remains obscure. In this study, bacteria were sequenced at 6 Zeitgeber times (ZT) from a pig model of ileal T-shaped fistula to test ileal bacterial composition and circadian rhythms after Lactobacillus delbrueckii administration. The results showed that dietary L. delbrueckii enhanced ileal bacterial α-diversity at Zeitgeber time (ZT) 16, evidenced by an increased Simpson index compared with control pigs. At the phylum level, Firmicutes was identified as the largest phyla represented in pigs, but dietary L. delbrueckii only increased the abundance of Tenericutes at ZT16. At the genus level, 11/100 genera (i.e., Lactobacillus, Enterococcus, Leptotrichia, Pediococcus, Bifidobacte, Cellulosilyticum, Desulfomicrobium, Sharpea, Eubacterium, Propionivibrio, and Aerococcus) were markedly differentiated in L. delbrueckii-fed pigs and the effect was rhythmicity-dependent. Meanwhile, dietary L. delbrueckii affected six pathways of bacterial functions, such as membrane transport, metabolism of cofactors and vitamins, cell motility, the endocrine system, signaling molecules and interaction, and the nervous system. Cosinor analysis was conducted to test bacterial circadian rhythm in pigs, while no significant circadian rhythm in bacterial α-diversity and phyla composition was observed. Lactobacillus, Terrisporobacter, and Weissella exhibited significant rhythmic fluctuation in the control pigs, which was disturbed by probiotic exposure. In addition, dietary L. delbrueckii affected circadian rhythms in ileal Romboutsia, Erysipelatoclostridium, Cellulosilyticum, and Eubacterium abundances. Dietary L. delbrueckii affected both ileal bacterial composition and circadian rhythms, which might further regulate gut function and host metabolism in pigs.
关键词：
Agricultural green production technology adoption;Market incentives;Perceived benefits;Perceived risks;Perceived value;Policy subsidies
摘要：
Understanding the underlying reasons for farmers' AGPT adoption in the context of resource environment tightening and agricultural carbon reduction has become crucial to promote agriculture sustainable development. This paper uses multiple ordered regression model and probit model to test the impact of farmers' perceived value on their adoption of agricultural green production technologies (AGPT) by using the first-hand data from 613 rice farmers in Hunan, and explores the effect and mechanism of policy subsidies and market incentives on the adoption of AGPT, and investigates the differences in the impact of perceived value on the adoption of green technologies in different production stages. The results showed that, (1)farmers' AGPT adoption is significantly affected by their perceived value, in which perceived benefits significantly promotes farmers' AGPT adoption, while the perceived risks is the opposite. But farmers' AGPT adoption in different production stages is influenced by different perceived value. The adoption of soil testing and fertilization technology (STFT) in the pre-production stage is significantly influenced by the perceived benefits; adoption of green pest control technology (GPCT) in the mid-production stage is significantly influenced by both the perceived benefits and the perceived risks; and adoption of straw return technology (SRT) in the post-production stage is significantly influenced by the perceived risks. (2) Farmers' adoption of green technologies can be encouraged by policy subsidies, which also have the moderating effect of decreasing the negative influence of perceived risks on farmers' adoption behaviour. (3) Further analysis reveals that market incentives can compensate for the limitations of policy subsidies, greatly promote farmers' adoption of AGPT, and regulate farmers' perceived risks and perceived benefits to encourage them to use green technologies. Consequently, the Government should actively publicize and organize training on agriculture green technologies, and provide diversified subsidy programmes for different green technologies. And governments should also improve the quality certification system and the market price mechanism for agricultural products, so as to help farmers adopting green technologies to achieve an increase in their income.
期刊：
Chemical Communications,2024年60(21):2906-2909 ISSN：1359-7345
通讯作者：
Xie, ZY;Wu, XW;Liao, JJ;Wu, Xiongwei;Ling, W
作者机构：
[Wu, Xuewen; Xie, Zhiyong] Cent South Univ, State Key Lab Powder Met, Changsha 410083, Peoples R China.;[Wu, Xuewen; Xie, Zhiyong] Cent South Univ, Sci & Technol High Strength Struct Mat Lab, Changsha 410083, Peoples R China.;[Wu, Xiongwei; Wu, Xuewen; Wu, Saixiang; Wu, XW; Yin, Xingrong; Liu, Jun] Hunan Prov YinFeng New Energy Co Ltd, Changsha 410000, Peoples R China.;[Liao, Jingjing] Univ South China, Sch Nucl Sci & Technol, Hengyang 421001, Peoples R China.;[Wu, Xiongwei; Ling, Wei; Wu, XW; Ling, W; Liu, Jun] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.
通讯机构：
[Xie, ZY ] C;[Wu, XW; Wu, XW ; Ling, W ] H;[Liao, JJ ] U;Cent South Univ, State Key Lab Powder Met, Changsha 410083, Peoples R China.;Cent South Univ, Sci & Technol High Strength Struct Mat Lab, Changsha 410083, Peoples R China.
摘要：
A phosphoric acid additive with an optimal concentration of 0.1 M can vastly promote the diffusion kinetics of the redox reaction between V(iv) and V(v) without a significant decline in energy efficiency for 300 cycles, and maintain the high-temperature stability (55 degrees C) of an electrolyte at a high state of charge (SOC) of 70% over the course of 30 days. A phosphoric acid additive with an optimal concentration of 0.1 M can maintain the high-temperature stability (55 degrees C) of an electrolyte at a high state of charge (SOC) of 70% over the course of 30 days.
作者机构：
[Yang, JL; Lu, Bingyi; Yang, Jinlong] Shenzhen Univ, Coll Mat Sci & Engn, Shenzhen Key Lab Energy Electrocatalyt Mat, Guangdong Res Ctr Interfacial Engn Funct Mat, Shenzhen 518060, Peoples R China.;[Lu, Bingyi] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen 518060, Peoples R China.;[Zhou, Guangmin; Xiao, Xiao; Lao, Zhoujie; Lu, Bingyi; Liu, Yingqi; Zhou, GM; Wu, Xinru] Tsinghua Univ, Tsinghua Berkeley Shenzhen Inst, Shenzhen 518055, Peoples R China.;[Zhou, Guangmin; Xiao, Xiao; Lao, Zhoujie; Lu, Bingyi; Liu, Yingqi; Zhou, GM; Wu, Xinru] Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China.;[Chen, Biao] Tianjin Univ, Sch Mat Sci & Engn, Tianjin 300350, Peoples R China.
通讯机构：
[Yang, JL ] S;[Zhou, GM ] T;Shenzhen Univ, Coll Mat Sci & Engn, Shenzhen Key Lab Energy Electrocatalyt Mat, Guangdong Res Ctr Interfacial Engn Funct Mat, Shenzhen 518060, Peoples R China.;Tsinghua Univ, Tsinghua Berkeley Shenzhen Inst, Shenzhen 518055, Peoples R China.;Tsinghua Univ, Tsinghua Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China.
关键词：
Li-CO2 batteries;bidirectional catalyst;coulombic efficiency;energy band engineering
摘要：
Abstract Li‐CO2 batteries have aroused great interest in the context of carbon neutralization, but their practicability is severely hindered by the sluggish CO2 redox reaction kinetics at the cathode, which brings about formidable challenges such as high overpotential and low Coulombic efficiency. For the complex multi‐electron transfer process, the design of catalysts at the molecular or atomic level and the understanding of the relationship between electron state and performance are essential for the CO2 redox. However, little attention has been paid to it. In this work, using Co3S4 as a model system, density functional theory calculations reveal that the adjusted d‐band and p‐band centers of Co3S4 with the introduction of Cu and sulfur vacancies are hybridized between CO2 and Li species, respectively, which is conducive to the adsorption of reactants and the decomposition of Li2CO3, and the experimental results further verify the effectiveness of energy band engineering. As a result, a highly efficient bidirectional catalyst is produced, and shows an ultra‐small voltage gap of 0.73 V and marvelous Coulombic efficiency of 92.6%, surpassing to those of previous catalysts under similar conditions. This work presents an effective catalyst design and affords new insight into the high‐performance cathode catalyst materials for Li‐CO2 batteries. This article is protected by copyright. All rights reserved
