通讯机构：
[Qian, W.] I;Institute of Vegetables and Flowers, 12 Zhongguancun Nandajie, China
关键词：
RNA-seq;dioecious;dioïque;détermination du sexe des fleurs;développement floral;floral sex determination;flower development;spinach;épinards
摘要：
Spinach (Spinacia oleracea L.) is commonly considered a dioecious plant with heterogametic (XY) and homogametic (XX) sex chromosomes. The characteristic is also utilized for the production of spinach hybrid seeds. However, the molecular mechanisms of sex determination in spinach are still unclear because of a lack of genomic and transcriptomic information. In this study, RNA sequencing (RNA-seq) was performed in male and female inflorescences to provide insight into the molecular basis of sex determination in spinach. Comparative transcriptome analyses showed that 2278 differentially expressed genes (DEGs) were identified between male and female inflorescences. A high correlation between the RNA-Seq and qRT-PCR validation for DEGs was observed. Among these, 182 DEGs were annotated to transcription factors including the MYB family protein, bHLH family, and MADS family, suggesting these factors might play a vital role in sex determination. Moreover, 26 DEGs related to flower development, including nine ABCE class genes, were detected. Expression analyses of hormone pathways showed that brassinosteroids may be key hormones related to sex determination in spinach. Overall, this study provides a large amount of DEGs related to sexual expression and lays a foundation for unraveling the regulatory mechanism of sex determination in spinach.
作者机构：
[Long, Xiaojie; Tang, Hongjiu] Hunan Agr Univ, Coll Landscape Architecture & Art Design, Changsha, Peoples R China.;[Tang, Hongjiu] Sun Yat Sen Univ, Sch Geog & Planning, Guangzhou, Peoples R China.;[Liu, Zhaoyin] Hunan Agr Univ, Coll Resources & Environm, Changsha, Peoples R China.
摘要：
This study examines farmers' intentions towards pro-environmental behavior in a famous tourist village in China called Guanshan, whose ecological environment is polluted. By adopting the empirically validated norm activation model (NAM) of Schwartz and merging it with Vroom's expectancy theory, the current research aims to develop a refined framework for understanding the formation of and predicting changes in pro-environmental intention. Field surveys were conducted in Guanshan, which resulted in sample data consisting of 275 valid responses collected by the research team. We develop a refined model, including six latent variables and 24 observational items. The structural equation modeling results show that the final model enjoys a better predictive accuracy of pro-environmental intention than does the original NAM. The study also discovers that the motivational force of expectancy theory significantly influences pro-environmental intention, whose motivational force comes from the impact of valence and expectancy. The pro-environmental intentions of farmers are mainly affected by the environmental personal norm and by a certain degree of personal expectancy. The improvement of farmers' pro-environmental intention needs be promoted in two approaches: the cultivation of personal environmental protection norms and the guidance of producing a desired expectation for pro-environmental intention.
通讯机构：
[Qian Zhao; Xingbo Shi] L;Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
关键词：
Prussian blue nanoparticles;multimode signals;point-of-care testing;aflatoxin B1;aptamer
作者：
Zhu, Jun Zi;Guo, Jun;Hu, Zhao;Zhang, Xu Tong;Li, Xiao Gang;...
期刊：
Frontiers in Microbiology,2021年12:653809 ISSN：1664-302X
通讯作者：
Zhong, J.;Li, X.G.
作者机构：
[Zhu, Jun Zi; Li, Xiao Gang] Hunan Agr Univ, Hunan Engn Res Ctr Agr Pest Early Warning & Contr, Changsha, Peoples R China.;[Zhang, Xu Tong; Zhong, Jie; Guo, Jun; Hu, Zhao; Zhu, Jun Zi; Li, Xiao Gang] Hunan Agr Univ, Prov Key Lab Biol & Control Plant Dis & Insect Pe, Changsha, Peoples R China.
通讯机构：
[Li, X.G.; Zhong, J.] H;Hunan Engineering Research Center of Agricultural Pest Early Warning and Control, China;Hunan Provincial Key Laboratory for Biology, China
摘要：
Here, we report a novel double-stranded RNA virus designated Colletotrichum liriopes partitivirus 1 (ClPV1) from the plant pathogenic fungus C. liriopes. ClPV1 genome has two double stranded RNAs (dsRNAs), named as dsRNA 1 and dsRNA 2, which in the lengths of 1,807 and 1,706 bp, respectively. The dsRNA 1 and dsRNA 2 encoded proteins showing significant amino acid (aa) sequence identity to the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of partitiviruses, respectively. Phylogenetic analysis using the aa sequences of RdRp and CP indicated that ClPV1 was grouped to members of the putative Epsilonpartitivirus genus in the Partitiviridae family. Spherical viral particles in approximately 35 nm in diameter and packaging the ClPV1 genome were isolated. Virus elimination and virus transfection with purified viral particles, and biological comparison revealed that ClPV1 could reduce the virulence and conidia production of C. liriopes. To the best of our knowledge, this is the first report of mycovirus in C. liriopes fungus.
通讯机构：
[Yuanshan Lin] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
摘要：
Cellulase plays an important role in addressing the issue of the energy crisis. However, the yield and degradation efficiency of cellulase remain a major challenge. In the present study, we aimed to verify whether ammonium ion (NH4+) could induce cellulase synthesis from T. koningii AS3.2774 and to explore new functional genes related to the cellulase production. Our results indicated that NH4+ induces cellulase production in a way different from nitrogen sources. NH4+-mediated mycelia displayed a significant increase in transport vesicles. Under NH4+ mediation, CBHI, CBHII, glycoside hydrolase family 5 proteins, Hap2/3/5 complexes, “ribosome biogenesis”, and “heme binding” were significantly up-regulated, and differentially expressed genes (DEGs) were mainly involved in “Metabolism”. Collectively, our findings illustrated that NH4+ induced the cellulase production at morphological and gene expression levels, which might be related to the Hap2/3/5 complex, ribosomes, and genes involved in various amino acid metabolism, pyruvate metabolism, and glycolysis/gluconeogenesis. Taken together, our results provided valuable insights into the regulatory network of cellulase gene expression in filamentous fungi.
通讯机构：
[Longbo Jiang; Xingzhong Yuan] C;College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China<&wdkj&>Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong<&wdkj&>College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China
摘要：
Graphitic carbon nitride (g-C3N4), as a significant metal-free photocatalyst, has elicited ripples of excitement due to its many extraordinary features, such as its mild bandgap, high thermal and chemical stability, inexpansive, and environmental friendly. However, the unsatisfactory solar light absorption, low surface area and the rapid recombination of photogenerated charges severely restrict the photocatalytic activity of bulk g-C3N4. Especially, g-C3N4 with a bandgap of 2.7 eV means an absorption threshold at about 450 nm that is still insufficient for efficient visible light capturing and leaves the near-infrared (NIR) light spectrum unexploited. In order to facilitate future material design for efficient g-C3N4 photocatalysts under solar light (UV similar to 5%, visible light similar to 43%, NIR light similar to 52%), we reviewed the recent progress of NIR-driven g-C3N4 based photocatalysts. Many strategies, including combination of narrow optical gap materials, bandgap engineering, upconversion materials, plasmonic materials, and photosensitizers, have been summarized to broaden the light absorption of g-C3N4 to NIR light region. Besides, the diverse photocatalytic applications of NIR driven g-C3N4 photocatalysts have been summarized, including water purification, water splitting, N-2 photofixation, CO2 reduction, NO removal, H2O2 production, bacteria disinfection, photodynamic therapy and organic synthesis, etc. The mechanism and the roles of various strategies in the NIR photocatalytic process were highlighted in details. Moreover, the limitations and possible solutions for each method are discussed. (C) 2021 Elsevier B.V. All rights reserved.
摘要：
Yellow peel will adversely affect the appearance quality of cucumber fruit, but the metabolites and the molecular mechanism of pigment accumulation in cucumber peel remain unclear. Flavonoid metabolome and transcriptome analyses were carried out on the young peel and old peel of the color mutant L19 and the near-isogenic line L14. The results showed that there were 165 differential flavonoid metabolites in the old peel between L14 and L19. The total content of representative flavonoid metabolites in the old peel of L14 was 95 times that of L19, and 35 times that of young peel of L14, respectively. This might explain the difference of pigment accumulation in yellow peel. Furthermore, transcriptome analysis showed that there were 3396 and 1115 differentially expressed genes in the yellow color difference group (Young L14 vs. Old L14 and Old L14 vs. Old L19), respectively. These differentially expressed genes were significantly enriched in the MAPK signaling pathway–plant, plant–pathogen interaction, flavonoid biosynthesis and cutin, suberine and wax biosynthesis pathways. By analyzing the correlation between differential metabolites and differentially expressed genes, six candidate genes related to the synthesis of glycitein, kaempferol and homoeriodictyol are potentially important. In addition, four key transcription factors that belong to R2R3-MYB, bHLH51 and WRKY23 might be the major drivers of transcriptional changes in the peel between L14 and L19. Then, the expression patterns of these important genes were confirmed by qRT-PCR. These results suggested that the biosynthesis pathway of homoeriodictyol was a novel way to affect the yellowing of cucumber peel. Together, the results of this study provide a research basis for the biosynthesis and regulation of flavonoids in cucumber peel and form a significant step towards identifying the molecular mechanism of cucumber peel yellowing.
通讯机构：
[Chao Xu] K;[Xiangdong Zhu] C;Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China<&wdkj&>College of Biological Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China
摘要：
Paddy soil contaminated by cadmium (Cd) has attracted worldwide attention, while foliar spraying of zinc (Zn) could be considered a cost-effective and practical agronomic measure for reducing Cd accumulation in rice grain. However, the effects due to foliar spraying of Zn on different cultivars, as well as the mechanism of subsequent processes taking place are not fully understood up to now. To go a step ahead, a field experiment was conducted with the aim of studying the capability of foliar application of Zn (0.4% ZnSO4) to reduce Cd concentration in grain in five late rice cultivars (here named JLYHZ, FYY272, JY284, CLY7 and LXY130), and the antioxidant activities and subcellular distribution of Cd in the leaves. The results indicate that foliar Zn application significantly decreased grain yield in JY284, CLY7 and JLYHZ, compared to controls. In addition, foliar application of Zn significantly decreased Cd concentration in grain of the five rice cultivars, while increased Zn concentration. The effect of foliar application of Zn on transport coefficients of Cd varied greatly for the different rice cultivars. Foliar application of Zn significantly decreased the malondialdehyde (MDA) concentration in rice leaves, and increased peroxidase (POD) activity. Also, it changed the distribution of Cd in the soluble fraction in leaves (expressed as proportion), which was significantly decreased, and the proportion of Cd in the cell wall increased. The structural equation model (SEM) revealed the positive effects of flag leaf Cd, first node Cd, old leaf Cd, and root Cd concentration on grain Cd concentration. Flag leaf Cd had the highest standardized total effects on grain Cd concentration, followed by old leaf Cd. These results indicated that foliar application of Zn was effective in reducing grain Cd concentration of late rice by enhancing antioxidant activities and Cd chelation onto cell wall of leaves, and reducing Cd concentrations in leaves. (C) 2021 Elsevier B.V. All rights reserved.
摘要：
Heavy metal pollution affects soil ecological function. Biochar and compost can effectively remediate heavy metals and increase soil nutrients. The effects and mechanisms of biochar and compost amendments on soil nitrogen cycle function in heavy-metal contaminated soils are not fully understood. This study examined how biochar, compost, and their integrated use affected ammonia-oxidizing microorganisms in heavy metal polluted soil. Quantitative PCR was used to determine the abundance of ammonia-oxidizing archaea (AOA) and bacteria (AOB). Ammonia monooxygenase (AMO) activity was evaluated by the enzyme--linked immunosorbent assay. Results showed that compost rather than biochar improved nitrogen conversion in soil. Biochar, compost, or their integrated application significantly reduced the effective Zn and Cd speciation. Adding compost obviously increased As and Cu effective speciation, bacterial 16S rRNA abundance, and AMO activity. AOB, stimulated by compost addition, was significantly more abundant than AOA throughout remediation. Correlation analysis showed that AOB abundance positively correlated with NO3--N (r = 0.830, P < 0.01), and that AMO activity had significant correlation with EC (r = -0.908, P < 0.01) and water-soluble carbon (r = -0.868, P < 0.01). Those seem to be the most vital factors affecting AOB community and their function in heavy metal-polluted soil remediated by biochar and compost. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
作者：
Arshad, Rai Naveed;Abdul-Malek, Zulkurnain;Roobab, Ume;Munir, Muhammad Abdullah;Naderipour, Amirreza;...
期刊：
Trends in Food Science & Technology,2021年111:43-54 ISSN：0924-2244
通讯作者：
Aadil, R.M.;Abdul-Malek, Z.
作者机构：
[Abdul-Malek, Zulkurnain; Naderipour, Amirreza; Munir, Muhammad Abdullah; Arshad, Rai Naveed] Univ Teknol Malaysia, Inst High Voltage & High Current, Skudai 81310, Johor, Malaysia.;[Roobab, Ume] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China.;[Qureshi, Muhammad Imran] Tech Univ Malaysia, Fac Technol Management & Technopreneurship, Malacca 75300, Malaysia.;[Bekhit, Alaa El-Din] Univ Otago, Dept Food Sci, Dunedin 9054, New Zealand.;[Liu, Zhi-Wei] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.
通讯机构：
[Zulkurnain Abdul-Malek] I;[Rana Muhammad Aadil] N;Institute of High Voltage & High Current, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia<&wdkj&>National Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, Pakistan
关键词：
Economic sustainability;Energy and waste reduction;Food safety and security;Pulsed electric field;Revenue generation;Sustainability
摘要：
Tumor necrosis factors (TNFs) are a group of cytokines that play critical roles in regulating a diverse range of physiological processes in vertebrates. TNFs function by activating a large number of structurally related receptors, leading to TNF mediated biological processes which are evolutionarily conserved. Fish have a much diversified TNF family, partly due to the whole genome duplication events which have occurred in this lineage, providing an excellent model to investigate the neo- and sub-functionalised properties of TNF superfamily. Fish possess most of the TNFs and receptors found in mammals and also some homologues exclusively present in fish. It seems that TNFSF4 (OX40), TNFSF7 (CD27) and TNFSF8 (CD30) and their cognate receptors are absent in teleosts. It has been shown that fish viruses are able to produce TNFR homologues to establish infection by manipulating the host immune system. Understanding the roles of TNFSFs in fish immune defence and the pathogenesis of fish diseases will provide insights into the functions of TNFSFs from an evolutionary perspective and better strategies for improving fish health and welfare in aquaculture. This review summarises recent advances in the study offish TNF biology and focuses on the molecular properties and immunological functions of the TNF and TNFR superfamily.
摘要：
The prevalence and antimicrobial susceptibility of Clostridium perfringens (C. perfringens) in chickens and pigs were investigated in Beijing and Shanxi, China. In total, 322 C. perfringens (chicken n = 60 and pig n = 262) were obtained from 620 feces of chickens (n = 256) and pigs (n = 364). Multiplex PCR for toxin typing of C. perfringens revealed that all the isolates belong to type A, with 45.7 % (147/322) isolates carrying beta-2 toxin-encoding gene cpb2. Minimum inhibitory concentrations of 27 antimicrobial agents showed that 91.0 % of the tested C. perfringens isolates were resistant to gentamicin and sulfonamides (sulfisoxazole and trimethoprimsulfamethoxazole), and little resistance was showed to amoxicillin-clavulanate, ceftiofur, doxycycline, vancomycin and linezolid. Additionally, nosiheptide, avilamycin, virginiamycin and bacitracin exhibited good activity against the tested C. perfringens with low MIC50 (0.06 to <= 4 mu g/mL) and MIC90 values (0.25-8 mu g/mL). Whole genome sequencing (WGS) of 48 representative isolates from each farm indicated that the C. perfringens contained diverse antimicrobial resistance genes [tetA(P), ant(6)-Ib, erm(Q), etc.] and toxin genes (cpb2, colA, cloSI, pfoA, etc.). By comparative analysis, four C. perfringens isolates from three different pig farms harboured cpb2-carrying plasmid pl with 100 % nucleotide sequence identity, suggesting horizontal gene transfer among these microorganisms. The further phylogenomic reconstruction, based on the core-genome single nucleotide polymorphisms (SNPs) of the representatives, demonstrating that C. perfringens from the same farms and regions were closely related. These findings expanded our knowledge of C. perfringens isolated from animals in China, which provided scientific basis for efficient intervention or prevention measures of antimicrobial resistance in animal husbandry in China.
摘要：
The present study investigated the effect of eugenol (EUG) on dextran sulfate sodium (DSS)-induced colitis and explored the underlying mechanisms. C57BL/6 mice were intragastrically administered normal saline or EUG (20 mg/kg body weight) for 17 days, and colitis was induced by using 3% DSS from day 7. The results showed that EUG increased the body weight and reduced the disease activity index score and colon pathological scores in DSS-treated mice (P < 0.05). Further, EUG preserved the proinflammatory cytokines (interleukin (IL)-6, -12, -21, and -23), lowered (P < 0.05) colonic malondialdehyde (MDA), uncoupling protein 2 (UCP2) expression and p65 phosphorylation, and activated (P < 0.05) colonic kelch-like ECH-associated protein 1 and nuclear factor (erythroid-derived 2)-like 2 expressions but did not affect the intestinal microbiota in DSS-treated mice. Furthermore, EUG ameliorated colitis in antibiotic-treated mice, while fecal microbiota transplantation from EUG preadministered mice failed to ameliorate colitis. In conclusion, EUG could alleviate colitis by attenuating colonic inflammation and oxidative stress independent of intestinal microbiota.
摘要：
In this study, a novel mitovirus designed "Colletotrichum higginsianum mitovirus 1" (ChMV1) was isolated from the phytopathogenic fungus Colletotrichum higginsianum. The genome of this mitovirus is 2,893 nt in length with an A + U content of 61% and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). A BLASTp analysis revealed that the RdRp domain of ChMV1 had 30.25% to 61.72% sequence identity to those of members of the genus Mitovirus and showed the highest degree of similarity (61.72% identity) to Botrytis cinerea mitovirus 3 (BcMV3). Phylogenetic analysis further indicated that ChMV1 is a member in the genus Mitovirus of the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus in C. higginsianum.
关键词：
Total volatile basic nitrogen;Au@MnO2 nanocomposites;Sensing hydrogel;Fish freshness;RGB analysis
摘要：
Total volatile basic nitrogen (TVB-N) content is the most widely used index for the assessment of fish freshness. Herein, a smartphone-integrated colorimetric sensor of TVB-N is developed based on a functional hydrogel loaded with Au@MnO2 core-shell nanocomposites and beta-D-glucose pentaacetate (beta-D-GP). In the sensing hydrogel, Au@MnO2 serves as a colorimetric substance and beta-D-GP is a precursor of reducing agent. TVB-N can permeate the sensing hydrogel, causing an alkaline environment in which beta-D-GP is hydrolyzed to produce beta-D-glucose. A redox reaction between MnO2 and beta-D-glucose can occur and thus the TVB-N can induce the etching of MnO2 layer of the Au@MnO2. Owing to the change of localized surface plasma resonance (LSPR) effect, the color of AuNPs changing from purple to red is easily distinguished by the naked eye. To quantify the variation conveniently, the color information is digitized by a smartphone combined with RGB analysis. The hydrogel sensing platform is further used for real-time monitoring of fish spoilage successfully. The introduction of smartphone avoids the usage of expensive instrument and opens a practical way for cost-effective, non-invasive, and portable monitoring of foodstuff freshness.
通讯机构：
[Xin Wu] C;College of Resources and Environment, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, Hunan 410125, China<&wdkj&>Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330096, China
摘要：
This research aimed to study whether Enteromorpha polysaccharide-zinc (EP-Zn) can act as an alternative to antibiotics in weaned piglet feeds. Two hundred and twenty-four weaned piglets from 14 pens were randomly assigned into 1 of 2 groups according to their body weight and litter size (7 pens/group). The piglets in the antibiotics group were fed with olaquindox at 400 mg/kg and enduracidin at 800 mg/kg basal diet, and piglets in the EP-Zn group were fed with EP-Zn at 800 mg/kg basal diet. One piglet per pen was selected to collect samples after 14 d of feeding. Results showed that EP-Zn supplementation significantly increased the plasma anti-oxidants level compared with the antibiotics group. However, a nonsignificant difference was observed in growth performance between treatment groups. Additionally, the intestinal tight junction (TJ) protein expression and the histopathologic evaluation data showed that EP-Zn contributed to improving intestinal development. Further, piglets in the EP-Zn group had a lower level of intestinal inflammation-related cytokines including IL-6 (P < 0.001), IL-8 (P < 0.05), IL-12 (P < 0.05) and tumor necrosis factor-alpha (TNF-alpha) (P < 0.001), and showed an inhibition of the phosphorylation nuclear transcription factor-kappa B (peNFekB) (P < 0.05) and total NF-kB (P < 0.001) level in the jejunal mucosa. Taken together, it is supposed that EP-Zn, to some extent, would be a potent alternative to prophylactic antibiotics in improving the health status of weaned piglets. (C) 2021 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
通讯机构：
[Lei Liu; Yingfang Zhou] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
关键词：
animal metabolic diseases;fat metabolism;MTP;ApoB100;ApoE;perinatal period
摘要：
Fatty liver is closely associated with elevated concentrations of nonesterified fatty acids (NEFA) and a low level of very low-density lipoproteins (VLDL) in blood of dairy cows. High NEFA inhibit the VLDL synthesis and assembly, and cause hepatic triacylglycerol (TAG) deposition. Sirtuin 3 (SIRT3), a mitochondrial deacetylase, antagonizes NEFA-induced TAG accumulation through modulating expressions of fatty acid synthesis and oxidation genes in cow hepatocytes. However, the role of SIRT3 in the VLDL synthesis and assembly was largely unknown. Here we aimed to test whether SIRT3 would recover the synthesis and assembly of VLDL in cow hepatocytes induced by high NEFA. Primary cow hepatocytes were isolated from 3 Holstein cows. Hepatocytes were infected with SIRT3 overexpression adenovirus (Ad-SIRT3), SIRT3-short interfering (si) RNA, or first infected with Ad-SIRT3 and then incubated with 1.0 mM NEFA (Ad-SIRT3 + NEFA). Expressions of key genes in VLDL synthesis and the VLDL contents in cell culture supernatants were measured. SIRT3 overexpression significantly increased the mRNA abundance of microsomal triglyceride transfer protein (MTP), apolipoprotein B100 (ApoB100) and ApoE (p < 0.01), and raised VLDL contents in the supernatants (p < 0.01). However, SIRT3 silencing displayed a reverse effect in comparison to SIRT3 overexpression. Compared with NEFA treatment alone, the Ad-SIRT3 + NEFA significantly upregulated the mRNA abundance of MTP, ApoB100 and ApoE (p < 0.01), and increased VLDL contents in the supernatants (p < 0.01). Our data demonstrated that SIRT3 restored the synthesis and assembly of VLDL in cow hepatocytes challenged with NEFA, providing an in vitro basis for further investigations testing its feasibility against hepatic TAG accumulation in dairy cows during the perinatal period.
