作者机构：
[Zheng, Qin; Teng, Zhenning; Ye, Nenghui] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;[Teng, Zhenning; Zhang, Jianhua] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong 999077, Peoples R China.;[Teng, Zhenning; Zhang, Jianhua] Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong 999077, Peoples R China.;[Teng, Zhenning] Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China.;[Zhang, Jianhua; Ye, Nenghui] Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong 999077, Peoples R China.
通讯机构：
[Zhang, JH; Ye, NH ] H;[Zhang, JH ] C;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong 999077, Peoples R China.;Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong 999077, Peoples R China.
摘要：
The seed, a critical organ in higher plants, serves as a primary determinant of agricultural productivity, with its quality directly influencing crop yield. Improper storage conditions can diminish seed vigor, adversely affecting seed germination and seedling establishment. Therefore, understanding the seed-aging process and exploring strategies to enhance seed-aging resistance are paramount. In this study, we observed that seed aging during storage leads to a decline in seed vigor and can coincide with the accumulation of hydrogen peroxide (H2O2) in the radicle, resulting in compromised or uneven germination and asynchronous seedling emergence. We identified the abscisic acid (ABA) catabolism gene, abscisic acid 8 '-hydroxylase 2 (OsABA8ox2), as significantly induced by aging treatment. Interestingly, transgenic seeds overexpressing OsABA8ox2 exhibited reduced seed vigor, while gene knockout enhanced seed vigor, suggesting its role as a negative regulator. Similarly, seeds pretreated with ABA or diphenyleneiodonium chloride (DPI, an H2O2 inhibitor) showed increased resistance to aging, with more robust early seedling establishment. Both OsABA8ox2 mutant seeds and seeds pretreated with ABA or DPI displayed lower H2O2 content during aging treatment. Overall, our findings indicate that ABA mitigates rice seed aging by reducing H2O2 accumulation in the radicle. This study offers valuable germplasm resources and presents a novel approach to enhancing seed resistance against aging.
摘要：
Huanglongbing (HLB) is a devastating citrus disease that causes significant financial losses in the citrus industry. However, there is no cure by current control strategies. Damaged soils were observed in citrus orchards; root lesions and nematodes were observed in HLB-affected citrus tree roots. Bacillus subtilis (B. subtilis) makes a great contribution to pathogen control and has the potential to control HLB. Purpureocillium lilacinum (P. lilacinum) and Trichoderma harzianum (T. harzianum) can kill pathogenic organisms such as nematodes and Phytophthora spp. to protect roots. Restoration of the damaged soils and improvement of citrus root growth through the introduction of soil amendments and biocontrol agents (containing B. subtilis, P. lilacinum, and T. harzianum) may provide an efficient approach to controlling HLB. Results revealed that soil properties such as soil pH and organic matter content were improved. After three months of combination treatment of soil amendment and biocontrol agent through root drenches, the citrus grew new roots and its leaves changed from yellow to green. The percentage of HLB-positive citrus trees-Orah, Tangerine, and Navel Orange-decreased from 90 to 0%, 87.5% to 21.88%, and 81.25% to 0% over three years, respectively. Furthermore, the productivity of HLB-affected orchards was restored, and both production and quality saw significant improvement. These results suggested that combining soil remediation and biocontrol improved soil quality and protected citrus root growth, thus effectively controlling HLB.
摘要：
Cr3+-activated near-infrared (NIR) phosphors have great potential in plant lighting, noninvasive detection, iris recognition, etc. Herein, an aluminoborate host material with a wide band gap (E-g = 4.60 eV) and high Debye temperature is selected to prepare a NIR phosphor Al5BO9: Cr3+ (ABO: Cr3+). The phosphor exhibits typical intermediate crystal field luminescence and has a high degree of matching with plant phytochrome. The photoluminescence intensity at 150 degrees C retains 91.1 % at room temperature. In addition, the spectral redshift (730 nm-770 nm) is achieved through the crystal field modulation, which is beneficial to broaden its application scenarios, such as plant antiviral immunity, night vision and noninvasive detection. Finally, the rice cultivation experiment confirms that the supplement of NIR light promotes growth, stress resistance and leaf angle of rice seedlings.
通讯机构：
[Guanghui Chen; Yue Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>The Key Laboratory of Crop Germplasm Innovation and Resource Utilization of Hunan Province, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Department of Agronomy, College of Agronomy, Hunan Agricultural University, Changsha 410128, China
关键词：
diverse rice hybrids;fresh wet rice noodles;physicochemical in rice noodles;amylose content;gel consistency
期刊：
International Journal of Molecular Sciences,2023年24(3):2255- ISSN：1422-0067
通讯作者：
Jianhua Zhang<&wdkj&>Nenghui Ye
作者机构：
[Chen, Yinke; Teng, Zhenning; Duan, Meijuan; Meng, Shuan; Ye, Nenghui] Hunan Agr Univ, Coll Agr, Changsha 410128, Peoples R China.;[Teng, Zhenning] Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China.;[Duan, Meijuan; Ye, Nenghui] Hunan Agr Univ, Hunan Prov Key Lab Rice Stress Biol, Changsha 410128, Peoples R China.;[Zhang, Jianhua] Hong Kong Baptist Univ, Dept Biol, Hong Kong 999077, Peoples R China.
通讯机构：
[Jianhua Zhang; Nenghui Ye] A;Authors to whom correspondence should be addressed.<&wdkj&>Department of Biology, Hong Kong Baptist University, Hong Kong 999077, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Agriculture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Rice Stress Biology, Hunan Agricultural University, Changsha 410128, China
摘要：
Light, temperature, water, and fertilizer are arguably the most important environmental factors regulating crop growth and productivity. Environmental stimuli, including low light, extreme temperatures, and water stresses caused by climate change, affect crop growth and production and pose a growing threat to sustainable agriculture. Furthermore, soil salinity is another major environmental constraint affecting crop growth and threatening global food security. The grain filling stage is the final stage of growth and is also the most important stage in cereals, directly determining the grain weight and final yield. However, the grain filling process is extremely vulnerable to different environmental stimuli, especially for inferior spikelets. Given the importance of grain filling in cereals and the deterioration of environmental problems, understanding environmental stimuli and their effects on grain filling constitutes a major focus of crop research. In recent years, significant advances made in this field have led to a good description of the intricate mechanisms by which different environmental stimuli regulate grain filling, as well as approaches to adapt cereals to changing climate conditions and to give them better grain filling. In this review, the current environmental stimuli, their dose-response effect on grain filling, and the physiological and molecular mechanisms involved are discussed. Furthermore, what we can do to help cereal crops adapt to environmental stimuli is elaborated. Overall, we call for future research to delve deeper into the gene function-related research and the commercialization of gene-edited crops. Meanwhile, smart agriculture is the development trend of the future agriculture under environmental stimuli.
摘要：
Gibberellin regulates plant growth, development, and metabolic processes. However, the underlying mechanism of the substantial effect of gibberellin on stem height and secondary metabolites in forage ramie is unclear. Therefore, this study combined transcriptomic and metabolomics analyses to identify the mechanisms regulating growth and secondary metabolite contents in forage ramie following exogenous gibberellin application. Exogenous gibberellin application significantly reduced the lignin content in the leaves but not in the stems. At the same time, gibberellin significantly increased the total flavonoid and chlorogenic acid contents in both the stems and leaves. In addition, 293 differentially expressed genes (DEGs) and 68 differentially expressed metabolites (DEMs) were identified in the leaves. In the stems, 128 DEGs and 41 DEMs were identified. The DEGs PER42, FLS, CYP75A, and PNC1 were up-regulated in the leaves, affecting phenylpropane metabolism. The joint analysis of the DEMs and DEGs revealed that the changes in the DEGs and DEMs in the leaves and stems improved the substrate efficiency in the phenol propane pathway and inhibited lignin synthesis in plants, thus shifting to flavonoid pathway synthesis. In conclusion, gibberellin treatment effectively reduces the lignin content in forage ramie while increasing the flavonoid and chlorogenic acid contents. These findings provide empirical and practical guidance for breeding for forage quality in ramie and the improvement and cultivation control of forage ramie.
通讯机构：
[Zhenxie Yi] C;College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
The objective was to determine effects of cellulase, xylanase, and commercial fibrolytic enzymes on fermentation quality, aerobic stability, bacterial community, and in vitro degradation of mixed silages. Mixtures of alfalfa, wheat bran, and rice straw [80:15:5 on a fresh matter (FM) basis] were ensiled for 1, 3, 5, 7, 15, 30, and 45 d after treatment with: distilled water (control, C); cellulase (E); xylanase (X); or commercial fibrolytic enzymes (EX), with all enzyme preparations applied at 100 U/g FM. The 45-day silages were subjected to an in vitro degradation test. Each of the three enzyme-treated groups enriched relative abundance (RA) of Lactobacillus, Weissella, and Stenotrophomonas maltophilia, increased water soluble carbohydrate (WSC) concentrations, and extended aerobic stability over 384 h, but concurrently inhibited growth of undesirable microbes (i.e., Acinetobacter sp, Lelliottia amnigena, and Sphingomonas sp), reducing pH and concentrations of ammonia nitrogen (AN), butyric acid (BA) and propionic acid (PA). Compared to C, adding X or EX increased the RA of L. paralimentarius and L. parabrevis, enhanced accumulation of acetic acid (AA) and crude protein (CP), and reduced hemicellulose content. Furthermore, E group silage had the highest abundance of W. cibaria. In addition, EX enriched RA of Bacillus velezensis, reduced AN concentration, increased DM degradability, total VFA production, and gas production during in vitro incubation. In conclusion, addition of X or EX enhanced ensiling by enhancing concentrations of AA; however, EX was the most promising enzyme, based on reducing AN concentration and increasing DM content and DM degradability.
作者机构：
[Wu, Ya; Zeng, Zaohai; Xia, Rui; Liu, Yuanlong; He, Yehua; Chen, Chengjie; Xu, Jing; Chen, CJ] South China Agr Univ, Coll Hort, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510640, Guangdong, Peoples R China.;[Zeng, Zaohai; Xia, Rui; Liu, Yuanlong; Chen, Chengjie; Xu, Jing; Chen, CJ] South China Agr Univ, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr & Rural Affair, Guangzhou 510640, Guangdong, Peoples R China.;[Zeng, Zaohai; Xia, Rui; Liu, Yuanlong; Chen, Chengjie; Xu, Jing; Chen, CJ] South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510640, Guangdong, Peoples R China.;[Li, Jiawei] Jinan Univ, Guangdong Hongkong Macau Inst CNS Regenerat, Guangdong Key Lab Nonhuman Primate Res, Guangzhou 510632, Guangdong, Peoples R China.;[Wang, Xiao] Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Henan Joint Int Lab Crop MultiOm Res, Kaifeng 475004, Peoples R China.
通讯机构：
[Chen, CJ; Xia, R ] S;South China Agr Univ, Coll Hort, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510640, Guangdong, Peoples R China.;South China Agr Univ, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr & Rural Affair, Guangzhou 510640, Guangdong, Peoples R China.;South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510640, Guangdong, Peoples R China.
关键词：
BSA-seq;TBtools-II;biological big data;plugin
摘要：
Since the official release of the stand-alone bioinformatics toolkit TBtools in 2020, its superior functionality in data analysis has been demonstrated by its widespread adoption by many thousands of users and references in more than 5000 academic articles. Now, TBtools is a commonly used tool in biological laboratories. Over the past 3 years, thanks to invaluable feedback and suggestions from numerous users, we have optimized and expanded the functionality of the toolkit, leading to the development of an upgraded version-TBtools-II. In this upgrade, we have incorporated over 100 new features, such as those for comparative genomics analysis, phylogenetic analysis, and data visualization. Meanwhile, to better meet the increasing needs of personalized data analysis, we have launched the plugin mode, which enables users to develop their own plugins and manage their selection, installation, and removal according to individual needs. To date, the plugin store has amassed over 50 plugins, with more than half of them being independently developed and contributed by TBtools users. These plugins offer a range of data analysis options including co-expression network analysis, single-cell data analysis, and bulked segregant analysis sequencing data analysis. Overall, TBtools is now transforming from a stand-alone software to a comprehensive bioinformatics platform of a vibrant and cooperative community in which users are also developers and contributors. By promoting the theme"one for all, all for one", we believe that TBtools-II will greatly benefit more biological researchers in this big-data era.
摘要：
MYB transcription factors have been demonstrated to play an important role in plant growth, development and abiotic stresses. This study isolated a rice MYB gene, OsMYB1R1(Os04g0583900), and functionally characterized its role in tolerance to drought stress by generating transgenic rice plants with overexpressing (OE) and RNA interference (RNAi) OsMYB1R1. Expression of OsMYB1R1 was down-regulated by drought stress. The tissue-specific expression analysis indicated that OsMYB1R1 was expressed at high level in panicle, but relatively low in the other parts of rice. No difference in germination rate among OsMYB1R1-OE, RNAi and wild-type (WT) seeds under mannitol treatments. No differences in phenotypes, physiological indicators and agronomic traits among WT, OE and RNAi plants were observed under normal grown conditions. Under drought stress, the RNAi plants were more tolerant to drought stress and higher survival rate after re-watering than WT plants. However, the overexpressing plants have found just the opposite. The OsMYB1R1-OE plants exhibited increased relative electrical conductivity (REC), increased malondialdehyde (MDA) content, and decreased proline content compared with the wild type, whereas lower REC and MDA content and higher proline content were found in the RNAi plants. These results suggest that OsMYB1R1 functions, a negative regulator in response to drought stresses, may be used as a candidate gene for molecular breeding of drought-tolerant crop varieties.
通讯机构：
[Yunling Peng] G;Gansu Provincial Key Lab of Arid Land Crop Science, Lanzhou 730070, China<&wdkj&>Author to whom correspondence should be addressed.
关键词：
GO analysis;maize;GSEA;SNP;PCR;drought tolerant
摘要：
Drought stress is a significant abiotic factor influencing maize growth and development. Understanding the molecular mechanism of drought tolerance is critical to develop the drought tolerant genotype. The identification of the stress responsive gene is the first step to developing a drought tolerant genotype. The aim of the current research was to pinpoint the genes that are essential for conserved samples in maize drought tolerance. In the current study, inbred lines of maize, 478 and H21, a drought-tolerant and susceptible line, were cultivated in the field and various treatments were applied. The circumstances during the vegetative stage (severe drought, moderate drought and well-watered environments) and RNA sequencing were used to look into their origins. In 478, 68%, 48% and 32% of drought-responsive genes (DRGs) were found, with 63% of DRGs in moderate drought and severe drought conditions in H21, respectively. Gene ontology (GO) keywords were explicitly enriched in the DRGs of H21, which were considerably over-represented in the two lines. According to the results of the GSEA, "phenylpropanoid biosynthesis" was exclusively enriched in H21, but "starch and sucrose metabolism" and "plant hormone signal transduction" were enhanced in both of the two lines. Further investigation found that the various expression patterns of genes linked to the trehalose biosynthesis pathway, reactive oxygen scavenging, and transcription factors, may have a role in maize's ability to withstand drought. Our findings illuminate the molecular ways that respond to lack and offer gene resources for maize drought resistance. Similarly, SNP and correlation analysis gave us noticeable results that urged us to do the same kind of analysis on other crops. Additionally, we isolated particular transcription factors that could control the expression of genes associated to photosynthesis and leaf senescence. According to our findings, a key factor in tolerance is the equilibrium between the induction of leaf senescence and the preservation of photosynthesis under drought.
关键词：
heavy metal;sediment;anthropogenic disturbances;bioleaching;bioaugmentation
摘要：
Soil, sediment, and waters contaminated with heavy metals pose a serious threat to ecosystem function and human health, and microorganisms are an effective way to address this problem. In this work, sediments containing heavy metals (Cu, Pb, Zn, Mn, Cd, As) were treated differently (sterilized and unsterilized) and bio-enhanced leaching experiments were carried out with the addition of exogenous iron-oxidizing bacteria A. ferrooxidans and sulfur-oxidizing bacteria A. thiooxidans. The leaching of As, Cd, Cu, and Zn was higher in the unsterilized sediment at the beginning 10 days, while heavy metals leached more optimally in the later sterilized sediment. The leaching of Cd from sterilized sediments was favored by A. ferrooxidans compared to A. thiooxidans. Meanwhile, the microbial community structure was analyzed using 16S rRNA gene sequencing, which revealed that 53.4% of the bacteria were Proteobacteria, 26.22% were Bacteroidetes, 5.04% were Firmicutes, 4.67% were Chlamydomonas, and 4.08% were Acidobacteria. DCA analysis indicated that microorganisms abundance (diversity and Chao values) increased with time. Furthermore, network analysis showed that complex networks of interactions existed in the sediments. After adapting to the acidic environmental conditions, the growth of some locally dominant bacteria increased the microbial interactions, allowing more bacteria to participate in the network, making their connections stronger. This evidence points to a disruption in the microbial community structure and its diversity following artificial disturbance, which then develops again over time. These results could contribute to the understanding of the evolution of microbial communities in the ecosystem during the remediation of anthropogenically disturbed heavy metals.
通讯机构：
[Huang, H ] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;Hunan Engn Res Ctr Rice Field Ecol Planting & Bree, Changsha 410128, Peoples R China.
关键词：
Rice;Earthworm;Cadmium contamination;Symbiosis system
摘要：
Currently, the effects of earthworm inoculation on cadmium-contaminated rice field remain unclear. In this study, four treatments were tested, including rice monoculture (CK), earthworm inoculation with low density (L, 30 g/m(2)), middle density (M, 60 g/m(2)), and high density (H, 90 g/m(2)). The pot and field experiment were conducted in Hunan Province, China. In the pot experiment, the H treatment significantly decreased the available cadmium concentration in 0 similar to 20 cm soil by 5.21% similar to 16.51%, and the M treatment significantly decreased in 0 similar to 10 cm soil by 7.29% similar to 8.96%. The H treatment significantly decreased the total cadmium concentration in 0 similar to 5 cm soil by 10.36%. Moreover, the earthworm inoculation treatments significantly reduced cadmium accumulation in rice organs. In the field experiment, the M and H treatment decreased the available cadmium concentration in 0 similar to 20 cm soil by 14.05% similar to 47.52% and the H treatment decreased the total cadmium concentration in 0 similar to 20 cm soil by 0.78% similar to 5.75% although there was no significant difference. Furthermore, the earthworm inoculation treatments significantly decreased cadmium accumulation in part of rice organs. In conclusion, this study recommends that earthworm inoculation is an effective method of controlling cadmium contamination for rice production.
通讯机构：
[Qingyun Yan] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou 510006, China
关键词：
Interdomain interactions;Microbial community;Network complexity;Trophic status
摘要：
The maize LSCgene, encoding a large subunit of ribonucleotide reductase, modulates dNTP synthesis, subsidiary cell development and plant growth. The four-celled stomatal complex consists of a pair of guard cells (GCs) and two subsidiary cells (SCs) in grasses, which supports a fast adjustment of stomatal aperture. The formation and development of SCs are thus important for stomatal functionality. Here, we report a maize lost subsidiary cells (lsc) mutant, with many stomata lacking one or two SCs. The loss of SCs is supposed to have resulted from impeded subsidiary mother cell (SMC) polarization and asymmetrical division. Besides the defect in SCs, the lsc mutant also displays a dwarf morphology and pale and striped newly-grown leaves. LSC encodes a large subunit of ribonucleotide reductase (RNR), an enzyme involved in deoxyribonucleotides (dNTPs) synthesis. Consistently, the concentration of dNTPs and expression of genes involved in DNA replication, cell cycle progression, and SC development were significantly reduced in the lsc mutant compared with the wild-type B73 inbred line. Conversely, overexpression of maize LSC increased dNTP synthesis and promoted plant growth in both maize and Arabidopsis. Our data indicate that LSC regulates dNTP production and is required for SMC polarization, SC differentiation, and growth of maize.
摘要：
Plants number is an essential field phenotypic trait that affects the growth status and final quality of crop. In recent years, the integration of remote sensing technology and deep learning technology has provided a solution to the problem of crop plant counting in field. However, most of the previous studies have selected fixed crops (such as rice, wheat) for research, and few studies have reported the limitations in the application of this technology. In addition, as far as we know, there has been no report on the problem of ramie germplasm resources counting. In this study, in combination with DA (Data Augmentation) and three object detection algorithms, ramie germplasm resources were adopted to explore the accuracy of counting plant number under the condition of dense plant growth. The following functions were tested: (1) the influence of DA on the effect of plant counting; (2) the influence of ground sampling distance (GSD) on the effect of plant counting; (3) the influence of object detection algorithms on ramie detection object. The results showed that after the training sample was expanded by DA, the Precision of ramie plant counting model was increased by 6.630%. FCOS (Fully Convolutional One-Stage Object Detection) could perform better in small object and small sample data (Recall = 0.892, Precision = 0.819，RMSE = 0.089). It was necessary to ensure the consistency of GSD between training samples and verification samples for improving the accuracy of ramie plants counting. The ramie plant counting model has sufficient and stable ability to count ramie plants in the field, which can supplement the traditional manual counting method.
摘要：
Rice plants are highly sensitive to high-temperature stress, posing challenges to grain yield and quality. However, the impact of high temperatures on the quality of high-quality hybrid rice during the booting stage, as well as the differing effects of the booting and grain-filling stages on grain quality, are currently not well-known. Therefore, four high-quality hybrid rice were subjected to control (CK) and high-temperature stress during the booting (HT1) and grain-filling stages (HT2). Compared to the control, HT1 significantly reduced the spikelets panicle(-1) (16.1%), seed setting rate (67.5%), and grain weight (7.4%), while HT2 significantly reduced the seed setting rate (6.0%) and grain weight (7.4%). In terms of quality, both HT1 and HT2 significantly increased chalkiness, chalky grain rate, gelatinization temperature, peak viscosity (PV), trough viscosity (TV), final viscosity (FV), and protein content in most varieties, and significantly decreased grain length, grain width, total starch content, and amylose content. However, a comparison between HT1 and HT2 revealed that the increase in chalkiness, chalky grain rate, PV, TV, and FV was greater under HT2. HT1 resulted in a greater decrease in grain length, grain width, total starch content, and amylose content, as well as an increase in protein content. Additionally, HT1 led to a significant decrease in amylopectin content, which was not observed under HT2. Therefore, future efforts in breeding and cultivating high-quality hybrid rice should carefully account for the effects of high temperatures at different stages on both yield and quality.
期刊：
Frontiers in Genetics,2023年14:1133600 ISSN：1664-8021
通讯作者：
Tang, W.;Sun, L.
作者机构：
[Tang, Wenbang; Huang, Zijian] Hunan Agr Univ, Coll Agron, Changsha, Peoples R China.;[Lv, Zhaokun; Chen, Yibo; Wang, Jiurong; Zhu, Yuxing; Li, Sai; Sun, Liang; Deng, Huabing] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China.;[Tang, Wenbang; Tian, Yan] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Peoples R China.
通讯机构：
[Tang, W.] C;[Sun, L.] K;College of Agronomy, China;Key Laboratory of Agro-Ecological Processes in Subtropical Region, China
关键词：
mineral accumulation;rice;phenotypic normalization;QTL;Subspecies differentiation
摘要：
Rice (Oryza sativa L.) is a major staple food that provides not only dietary calories but also trace elements for the global inhabitants. The insufficiency of mineral nutrients and the potential accumulation of excessive toxic elements in grains pose risks to human health. The substantial natural variations in mineral accumulation in rice grains presents potentials for genetic improvements of rice via biofortifications of essential mineral nutrients and eliminations of toxic elements in grains. However, the genetic mechanisms underlying the natural variations in mineral accumulation have not been fully explored to date owing to unstable phenotypic variations, which are attributed to poor genetic performance and strong environmental effects. In this study, we first compared the genetic performance of different normalization approaches in determining the grain-Cd, grain-Mn, and grain-Zn variations in rice in different genetic populations. Then through quantitative trait loci (QTLs) identification in two rice inter-ectype populations, three QTLs, including qCd7, qMn3, and qZn7, were identified and the QTLs were found to exhibit allelic differentiation in the different ecotypes. Our results were expected to broaden our understanding for mineral accumulation in rice and propose the potential functional alleles that can be explored for further genetic improvement of rice.
