作者机构：
[DING Zhong; Wang Shuinan; NAMPHUENG Janthathang] College of Plant Protection, Hunan Provincial Key Laboratory for Biology and Control of Plant Disease and Insect Pests, Hunan Agricultural University, Changsha 410128, China;[Peng Deliang; Huang Wenkun] The Key Laboratory for Biology of Insect Pests and Plant Disease, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, Beijing 100193, China
会议名称：
第四届国际禾谷类线虫病害学术研讨会(The 4th International Cereal Nematode Initiative Workshop)
会议时间：
2013-8-22
会议地点：
北京
会议主办单位：
中国植物病理学会
会议论文集名称：
第四届国际禾谷类线虫病害学术研讨会(The 4th International Cereal Nematode Initiative Workshop)论文集
摘要：
A novel cyromazine molecularly imprinted polymeric microspheres were prepared by two-step seed swelling polymerization method in aqueous system. The imprinting process was carried out using polystyrene particles as the seed, cyromazine as the template molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker. Factors affecting the particle morphologies including emulsifier, dispersant, the water-oil ratio and etc., were optimized in order to obtain the molecularly imprinted polymeric microspheres with a final narrow particle diameter of 3-5 μm. The prepared molecularly imprinted polymeric microspheres were characterized and evaluated by scanning electron microscope, infrared absorption spectroscopy, mercury analyzer and equilibrium binding experiments, respectively. Scatchard analysis suggested cyromazine were recognized by the prepared molecularly imprinted polymeric microspheres with two classes of binding sites. The apparent maximum binding capacity and dissociation constant were calculated to be 1.25 μmol/g (Qmax1) and 1.27 μmol/L (Kd1) for high affinity site, whilst to be 2.84 μmol/g (Qmax2) and 10.02 μmol/L (K d2) for low affinity site, respectively. The cyromazine molecularly imprinted polymeric microspheres synthesised in this method could be used as a stationary phase for the selective analysis of cyromazine in real samples by high performance liquid chromatography.
摘要：
Our recent research revealed that pinewood nematode (PWN) possesses few genes encoding enzymes for degrading α-pinene, which is the main compound in pine resin. In this study, we examined the role of PWN microbiome in xenobiotics detoxification by metagenomic and bacteria culture analyses. Functional annotation of metagenomes illustrated that benzoate degradation and its related metabolisms may provide the main metabolic pathways for xenobiotics detoxification in the microbiome, which is obviously different from that in PWN that uses cytochrome P450 metabolism as the main pathway for detoxification. The metabolic pathway of degrading α-pinene is complete in microbiome, but incomplete in PWN genome. Experimental analysis demonstrated that most of tested cultivable bacteria can not only survive the stress of 0.4% α-pinene, but also utilize α-pinene as carbon source for their growth. Our results indicate that PWN and its microbiome have established a potentially mutualistic symbiotic relationship with complementary pathways in detoxification metabolism.
作者机构：
[Shen Chen; Yinping Mo; Wenjuan Wang; Jing Su; Bing Chen; Liexian Zeng; Xiaoyuan Zhu] The Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;[Shen Chen; Xiaoyuan Zhu] Guangdong Provincial Key Laboratory of High Technology For Plant Protection,Guangzhou 510640,China;[Liangying Dai] College of Biosafety Science and Technology,Hunan Agricultural University,Changsha 410128,China
作者：
Li, J. L.;Wang, L. F.;Wang, H. Y.;Bai, L. Y.;Yuan, Z. M.*
期刊：
Genetics and Molecular Research,2012年11(3):3431-3451 ISSN：1676-5680
通讯作者：
Yuan, Z. M.
作者机构：
[Wang, L. F.; Yuan, Z. M.; Li, J. L.] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha, Hunan, Peoples R China.;[Yuan, Z. M.; Li, J. L.; Bai, L. Y.] Hunan Agr Univ, Coll Biosafety Sci & Technol, Changsha, Hunan, Peoples R China.;[Wang, H. Y.] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA.
摘要：
Adriamycin is an antitumor agent and its amphipathic characteristic makes interaction at the level of the cellular surface possible. In this paper, we studied the insertion behaviour of adriamycin and its metallic derivative, triferric adriamycin i.e., quelamycin, into lipid membrane with vesicles and supported bilayer lipid membrane as model membranes formed by neutral lipids. Observed from UV-VIS absorption spectroscopy, quelamycin could interact with vesicles in athiamycin-iron complex form. In the fluorescence measurements, adriamycin and triferric adriamycin could insert into the bilayer membrane of vesicles. The concentration effect of adriamycin on the supported bilayer lipid membrane and comparison of the penetration ability between adriamycin and quelamycin were investigated through electrochemical methods with Fe(CN)(6)(4-/3-) as marker ion.
摘要：
This paper reports a novel colorimetric sensor for pymetrozine based on p-aminobenzenesulfonic acid functionalized silver nanoparticles (p-ABSA-modified Ag NPs), which were characterized by IR spectroscopy, ultraviolet-visible spectroscopy (UV-vis), and transmission electron microscopy (TEM). The newly synthesized p-ABSA-modified Ag NPs are yellow in color due to the intense surface plasmon absorption band centered at 400nm. In the presence of pymetrozine, the yellow p-ABSA-modified Ag NPs solution turns to dark green, with a dramatic surface plasmon absorption band centered at 660nm. Moreover, high selectivity for pymetrozine was approved by the comparative experiments with an absorption ratio of A660/A400 more than 0.7. This highly sensitive sensor allows a direct and rapid quantitative assay of pymetrozine with a colorimetric limited detection concentration of 0.01mg/L.
关键词：
age;biological control agent;copulation experience;mate choice;morphological trait;Ophraella communa
摘要：
Ophraella communa is a biological control agent of invasive common ragweed, Ambrosia artemisiifolia. To understand the mate choice tactic of the beetle and improve mass-rearing of high-quality populations, the effects of morphological traits, age and copulation experience on mate choice in this beetle were studied in the laboratory. The results showed thatmate choice of male or female was related to certain adult morphological traits. Wing length, black streak width of frons and metaleg femur length of males were central to female mate choice. Females with longer wing length, black streak width of frons, pronotum width and foreleg tibia length were more attractive to males than those with the smaller ones. The chosen rate of older males was significantly higher than those of younger ones, and the chosen rate of females reached a maximum value on the 8th day after eclosion. The newly copulated females were less attractive to males than virgins, but females that have copulated in the distant past were more attractive than or were similar to virgins. The chosen rates of virgin males were significantly higher than those of newly copulated and 8 days past copulated ones, but no difference between virgin males and 3, 5 or 12 days past copulated ones in O. communa. The results of our study have added much needed empirical data regarding the significance of morphological traits, age and copulation experience as a source of variation in insect copulation signals. In addition, the results provide valuable information for mass-rearing of high-quality populations of O. communa.
