摘要：
This study was conducted to test the hypothesis that the dietary glutamine supplementation had various effects on oedema disease induced by Escherichia coli (E. coli). Total of 162 KunMing mice were randomly assigned to glutamine group 1 (1.0% glutamine+basal diet, n = 45), glutamine group 2 (1.0% glutamine+basal diet, n = 45), control group 1 (1.22 % alanine+basal diet, n = 36) and control group 2 (1.22% alanine+basal diet, n = 36). After 3 days of adaptive feeding and a 7-days treatment period with the experiment feed, all mice were challenged by intraperitioneal injection of E. coli O-19 at LD50 (2.53 x 10(8) CFU/ml). The mortality, after twenty hours of injection was calculated from glutamine group 2 and control group 2. Serum concentrations of platelet-activating factor (PAF), interleukin (IL)-2, interleukin (IL)-10, secretory, immunoglobulin A (sIgA), superoxide dismutase (SOD) activity, cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) were measured in glutamine group 1 and control group 1 in a ten-hours interval for three times. Serum concentrations of PAF in 1.0% glutamine group were significantly lower (P<0.01) than that in 1.22% alanine group at ten and thirty hours after initial injection and lower (P<0.05) than the control group twenty hours after first injection. Additionally, the supplementation of glutamine increased the SOD activity significantly (P<0.05) in the first twenty hours after initial injection. However, the SOD activity in the glutamine group was lower (P<0.05) than in the control group later. Furthermore serum concentration of cAMP had no difference at the first ten hours alter injection, whereas the cAMP concentrations in the glutamine group was significantly lower P<0.01) at twenty hours after initial injection and lower (P<0.05) than in the control group thirty hours after first injection. Meanwhile, glutamine supplementation had little effect on the mortality of mice, serum IL-2, sIgA and cGMP. In conclusion, dietary glutamine supplementation could partially attenuate the damage caused by oedema disease, but had little effect on the clinical result.
作者：
Wang, A. P.;Zhang, J. M.*;Meng, Y. L.;Deng, L. Q.;Lv, Y. F.;...
期刊：
Journal of Dairy Science,2012年95(9):5127-5132 ISSN：0022-0302
通讯作者：
Zhang, J. M.
作者机构：
[Li, C.; Wang, J. Q.; Lv, Y. F.; Meng, Y. L.; Deng, L. Q.; Zhang, J. M.; Wang, A. P.] Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China.;[Wang, A. P.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Lv, Y. F.] S China Agr Univ, Coll Anim Sci & Technol, Guangzhou 510642, Guangdong, Peoples R China.
通讯机构：
[Zhang, J. M.] C;Chinese Acad Agr Sci, State Key Lab Anim Nutr, Inst Anim Sci, Beijing 100193, Peoples R China.
摘要：
Free gossypol residues in tissues or milk from feeding whole cottonseed and cottonseed meal were measured for their effect on health of dairy cows and humans. Forty lactating cows were randomly assigned to 5 treatments in a 60-d experiment to investigate the effects of sources and dietary level of gossypol on plasma and milk gossypol concentrations in lactating cows. Five experimental diets had identical net energy for lactation and crude protein content on a dry matter (DM) basis. Soybean meal was the main protein ingredient used in the control diet. Cottonseed meal (CSM) or whole cottonseed (WCS) substituted for part of the soybean meal in the other 4 diets. Gossypol levels in the 5 diets were 0 (control), 91.15 mg/kg of DM in CSM1, 117.31 mg/kg of DM in CSM2, 385.43 mg/kg of DM in WCS1, and 611.13 mg/kg in WCS2. Yields of 3.5% fat-corrected milk were significantly higher for cows in the WCS2 group; cows in the CSM1 and WCS1 groups showed no differences but both were numerically higher than the control and CSM2 groups. Milk protein concentration was lower for cows consuming WCS1 compared with the control group. Lactose concentration was lower for cows in the CSM2 group compared with the WCS2 group, but no differences were observed among other diets. Aspartate aminotransferase in serum was significantly higher for the WCS2 group compared with the control and WCS1 groups, but no difference was observed with the CSM1 and CSM2 groups. Concentrations of gossypol in plasma and milk of cows in the WCS1 and WCS2 groups were both higher than those of the other groups. No adverse effects were observed on cows fed diets containing 12.0% CSM, and no gossypol was found in plasma and milk. When WCS comprised 15% of the diet DM, yields of 3.5% fat-corrected milk were increased in cows and gossypol was detected in plasma and milk but not at harmful levels.
作者机构：
Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central,;College of Animal Science,Hunan Agricultural University,Changsha,Hunan,410128,China
会议名称：
生猪优质健康养殖关键技术国际学术研讨会
会议时间：
2012-10-16
会议地点：
长沙
会议论文集名称：
生猪优质健康养殖关键技术国际学术研讨会论文集
关键词：
glutamine;deoxynivalenol;stress;immune function
作者机构：
Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Centra1,;College of Animal Science,Hunan Agricultural University,Changsha,Hunan,410128,China;Jiangsu Enry-Exit Inspection & Quarantine Bureau,Nanjing,China 210001
摘要：
Weaning body leads to intestinal immunological stress and decreases nutrient digestion, absorption and metabolism(1-5). We tried to modulate immunological stress with oligosaccharide and elucidate the signal pathway. This study was conducted to investigate the effects of SBOS on plasma concentration of immunoglobulin and interleukin and TLR2-NF-kappa B signal pathway in weanling mini-piglets. Twelve piglets weaned at 21 day of age were randomly assigned into 0 or 0.5 g/kg of SBOS-supplemented group (6 piglets per group), respectively. At the end of a 14-day feeding period, concentrations of IgG, IgM and IL-2 in plasma and mRNA expression levels of TLR2, MyD88 and NF-kappa B p65 were measured. Results showed that the SBOS supplementation increased concentrations of immunoglobulins IgG (P<0.05) and IgM (P>0.05) in plasma, as well as cytokines (IL-2) (P<0.05), when compared with the control group; the expression level of TLR2 and MyD88 in the spleen, and of NF-kappa B p65 and MyD88 in the colon of the piglets fed SBOS-supplemented diet were lower (P<0.05), while NF-kappa Bp65 in the spleen and TLR2 in the colon had no significant difference in comparison with the control group. We conclude that SBOS influences intestinal and spleen immunity by TLR2-NF-kappa B signal pathway in weanling pigs.
