通讯机构：
[Zhou, Xinyu] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, 410128, Changsha City, Hunan Province, P.R. China.
摘要：
A novel polymycovirus isolated from the plant-pathogenic fungus Colletotrichum gloeosporioides was identified. The viral genome is composed of nine double-stranded RNA segments, ranging in size from 699 bp to 2,444 bp. With the exception of dsRNA5, which contains two open reading frames (ORF5-1 and ORF5-2), the other dsRNA segments each contain one ORF. The proteins encoded by ORFs 1-8 are homologous to the proteins encoded by ORFs 1-8 of Colletotrichum camelliae filamentous virus 1 (CcFV-1). The amino acid sequences of the RNA-dependent RNA polymerase (RdRp) encoded by ORF1 and the viral methyltransferase encoded by ORF3 share 87.6% and 83.3% identity with CcFV-1. The proline-alanine-serine-rich protein (PASrp) encoded by ORF4 shares 86.6% sequence identity with that of CcFV-1. The proteins encoded by ORFs 2, 5 - 1, 6, 7, and 8 share 86.6%, 82.5%, 89.0%, 45.7%, and 95.5% sequence identity, respectively, with the corresponding proteins of CcFV-1. dsRNA9 is a defective copy of dsRNA2 that lacks a stretch of 1556 bp (nt 519 to nt 2074). Phylogenetic analysis based on the RdRp protein indicated that the novel virus clustered with members of the family Polymycoviridae, and based on the above results, we have tentatively named it "Colletotrichum gloeosporioides polymycovirus virus 1" (CgPmV1). To our knowledge, this is the first report of a polymycovirus with a defective dsRNA genome in C. gloeosporioides.
作者机构：
[Li, Xiao-Gang; Zhong, Jie; Li, Ping; Zhu, Jun-Zi] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Peoples R China.;[Li, Xiao-Gang; Zhu, Jun-Zi] Hunan Agr Univ, Hunan Engn Res Ctr Agr Pest Early Warning & Contr, Nongda Rd 1, Changsha 410128, Peoples R China.
通讯机构：
[Jie Zhong; Xiao-Gang Li] A;Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Engineering Research Center of Agricultural Pest Early Warning and Control, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha 410128, China
作者：
Ya Wang;Jing Tian;Zihao Wang;Chaonan Li;Xiaogang Li
期刊：
ACS Agricultural Science and Technology,2022年2(3):534-545 ISSN：2692-1952
通讯作者：
Xiaogang Li
作者机构：
[Li C.; Tian J.; Wang Z.; Wang Y.] College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China;Hunan Prov. Engineering Technology Research Center for Bio Pesticide and Formulating Processing, Changsha, 410128, China;[Li X.] College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China, Hunan Prov. Engineering Technology Research Center for Bio Pesticide and Formulating Processing, Changsha, 410128, China
通讯机构：
[Li, X.] C;College of Plant Protection, China
关键词：
CDK1;cell cycle;cyclin B;Heliothis virescens ascovirus 3h;SeFB cell line
摘要：
Ascoviruses are double-stranded DNA viruses that are pathogenic to noctuid larvae. In vitro infection causes the cells to fail to replicate and proliferate normally. However, the molecular mechanisms are unclear. In this study, the transmission electron microscopydata of infected-Spodoptera exigua (Hübner) fat body cells (SeFB, IOZCAS-SpexII-A cells) showed that virions were internalized in phagocytic vesicles, but not in the nucleus. FACS of cell-cycle progression was performed in SeFB cells infected with Heliothis virescens ascovirus 3h (HvAV-3h). The cell cycle phase distributions of the SeFB cells were G(1) = 29.52 ± 1.10%, S = 30.33 ± 1.19%, and G(2) /M = 40.06 ± 0.75%. The cell culture doubling time was approximately 24 h. The G(1) , S, and G(2) /M phases were each approximately 8 h. The unsynchronized or synchronized cells were arrested at G(2) /M phase after infection with HvAV-3h. Our data also showed that cells with more than 4N DNA content appeared in the HvAV-3h-treated group. While the mRNA levels of cyclin B(1) , cyclin H, and cyclin-dependent kinase 1 (CDK1) were downregulated after HvAV-3h infection, the mRNA expression levels of cyclin A, cyclin D, and cyclin B(2) were not significantly changed. Western blotting results showed that the expression of cyclin B(1) and CDK1 in infected SeFB cells within 24 h postinfection(hpi), and HvAV-3h infection inhibited the expression of cyclin B(1) and CDK1 at 12-24 hpi. Overall, these data implied that HvAV-3h infection leads to an accumulation of cells in the G(2) /M phases by downregulating the expression of cyclin B(1) and CDK1.
作者机构：
[Rong SONG] Institute of Agricultural Environment and Ecology, Hunan Academy of Agricultural Sciences;[Wenwen JIANG] College of Horticulture, Hunan Agricultural University;[Bei YAN] College of Plant Protection, Hunan Agricultural University;Xintian County Hospital of Traditional Chinese Medicine;Xintian County People's Hospital
摘要：
In this paper, the seedling raising, cultivation, field management, pest and disease control, harvesting and processing of Paris polyphylla Smith under the forest in Hunan Province were scientifically standardized, to provide technical support for standardized cultivation and sustainable development of Paris polyphylla Smith industry in Hunan Province.
作者：
Toshiya Hirowatari*;Sadahisa Yagi;Cheng-Qing Liao;Guo-Hua Huang;Min Wang
期刊：
Journal of Asia-Pacific Biodiversity,2022年15(3):391-400 ISSN：2287-884X
通讯作者：
Toshiya Hirowatari
作者机构：
[Toshiya Hirowatari; Sadahisa Yagi] Entomological Laboratory, Kyushu University, Faculty of Agriculture, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan;[Cheng-Qing Liao; Guo-Hua Huang] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, PR China;[Min Wang] Department of Entomology, South China Agricultural University, Guangzhou 510640, Guangdong, China
通讯机构：
[Toshiya Hirowatari] E;Entomological Laboratory, Kyushu University, Faculty of Agriculture, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan
摘要：
Nemophora chrysoprasias (Meyrick, 1907), previously known only by the type series from Assam, India, was discovered in the Yunnan Province of China. This study is the first to illustrate the male and female genitalia of N. chrysoprasias. Despite their differing appearances, this species is determined to be related to N. rubrofascia Christoph, 1882 based on their morphological characters and DNA barcode sequences.
通讯机构：
[Jianping Chen; Jian Yang] A;Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha 410128, China<&wdkj&>State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
摘要：
Ubiquitination is a major post-translational modification (PTM) involved in almost all eukaryotic biological processes and plays an essential role in plant response to pathogen infection. However, to date, large-scale profiling of the changes in the ubiquitome in response to pathogens, especially viruses, in wheat has not been reported. This study aimed to identify the ubiquitinated proteins involved in Chinese wheat mosaic virus (CWMV) infection in wheat using a combination of affinity enrichment and high-resolution liquid chromatography-tandem mass spectroscopy. The potential biological functions of these ubiquitinated proteins were further analyzed using bioinformatics. A total of 2297 lysine ubiquitination sites in 1255 proteins were identified in wheat infected with CWMV, of which 350 lysine ubiquitination sites in 192 proteins were differentially expressed. These ubiquitinated proteins were related to metabolic processes, responses to stress and hormones, plant-pathogen interactions, and ribosome pathways, as assessed via Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. Furthermore, we found that the ubiquitination of Ta14-3-3 and TaHSP90, which are essential components of the innate immune system, was significantly enhanced during CWMV infection, which suggested that ubiquitination modification plays a vital role in the regulatory network of the host response to CWMV infection. In summary, our study puts forward a novel strategy for further probing the molecular mechanisms of CWMV infection. Our findings will inform future research to find better, innovative, and effective solutions to deal with CWMV infection in wheat, which is the most crucial and widely used cereal grain crop.
