作者： Wang, Juan;Li, Qinjin;Peng, Yuande;Yang, Huilin;Wang, Zhi*;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2019年112(1):335-340 ISSN：0022-0493

通讯作者： Wang, Zhi

作者机构： [Peng, Yuande; Wang, Zhi; Wang, Juan] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Yang, Huilin; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Li, Qinjin; Luo, Lin] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.;[Li, Qinjin] Hunan Womens Univ, Tourism Dept, Changsha 410000, Hunan, Peoples R China.;[Wang, Zhi] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] C;[Wang, Zhi] H;Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

关键词： PQCI sensor;Pardosa pseudoamulata;methamidophos;proteinase

摘要： A piezoelectric quartz crystal impedance (PQCI) sensor was used to investigate influences of the insecticide methamidophos on proteinase activity in midguts of the wolf spider, Pardosa pseudoamulata (Araneae: Lycosidae). Results from PQCI indicated that low-concentration dose methamidophos (0.008%) can activate the proteinase but high-concentration dose methamidophos (0.016-0.032%) can inhibit the enzyme activity. The changes in subcellular structure of spider midgut cells were also observed. Electron micrographs of spider midgut epithelial cells showed that the low-dose methamidophos did not visibly impact the structure of these cells. Conversely, high-concentration dose methamidophos led to severe changes in the cell structure, including the karyotheca dissolved, the nucleolus, and the endoplasmic reticulum disappeared. These may contribute to changes in proteinase activity of spider. This work documents a feasible method for rapid and reliable detection of proteinase activity.

语种： 英文

作者： Zhang, Jing;Wang, Qiming;Zhu, Zhonglin;Qian, Hongliang;Jiang, Feng;...

期刊： Chemical Engineering & Technology,2019年42(2):388-396 ISSN：0930-7516

通讯作者： Huang, Dechun;Liu, Wei

作者机构： [Zhang, Jing; Jiang, Feng; Wang, Zhixiang; Qian, Hongliang; Zhu, Zhonglin; Huang, Dechun] China Pharmaceut Univ, Sch Engn, 639 Longmian Ave, Nanjing 210009, Jiangsu, Peoples R China.;[Wang, Qiming] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Liu, Wei] Nanjing Normal Univ, Sch Energy & Mech Engn, 1 Wenyuan Rd, Nanjing 210009, Jiangsu, Peoples R China.

通讯机构： [Huang, Dechun] C;[Liu, Wei] N;China Pharmaceut Univ, Sch Engn, 639 Longmian Ave, Nanjing 210009, Jiangsu, Peoples R China.;Nanjing Normal Univ, Sch Energy & Mech Engn, 1 Wenyuan Rd, Nanjing 210009, Jiangsu, Peoples R China.

关键词： Cosolvent;Gefitinib;Microparticle;Solubility;Solution-enhanced dispersion by supercritical fluids

摘要： The antineoplastic gefitinib has a low aqueous solubility, leading to poor absorption rates. To overcome this problem, microparticles (MP) were prepared using solution-enhanced dispersion by supercritical fluids (SEDS) with supercritical CO2 and the cosolvents dichloromethane and ethanol. The results showed that the use of SEDS resulted in the formation of smaller particles and rendered the usually heterogeneous crystals of the crude drug pure and uniform. Furthermore, the reduction in the MP size increased the dissolution rate of gefitinib, and in vitro cytotoxicity assays indicated that the MP inhibited the proliferation of A549 cells to a larger extent than did the crude drug. Given the beneficial properties of the MP, SEDS could potentially be used to micronize drugs for therapeutic applications. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

语种： 英文

作者： Wang, Juan;Lv, Zhiyue;Lei, Ziyan;Chen, Zhaoyang;Lv, Bo;...

期刊： Ecotoxicology and Environmental Safety,2019年172:19-25 ISSN：0147-6513

通讯作者： Wang, Zhi

作者机构： [Wang, Zhi; Wang, Juan] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Yang, Huilin; Chen, Zhaoyang; Lei, Ziyan; Lv, Bo; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

关键词： Cd transporters;Cuticle;Cytochrome P450 enzymes;Ecdysteroids;Molting

摘要： Cytochrome P450 enzymes (CYPs), encoded by Halloween genes, mediate the biosynthesis of molting hormone, ecdysteroids, in arthropods. In this report, the effect of heavy metal cadmium (Cd) stress on the expression of cytochrome P450 genes in the wolf spider Pardosa pseudoannulata was analyzed. The results showed the expression levels of genes encoding for Cd transporters including ABC transporters, zinc transporters, calcium channel proteins and calcium binding proteins were inhibited or induced by Cd stress. In addition, the increase in metallothionein (MT) content and glutathione peroxidase (GPX) activity and decrease in total acetylcholine esterase (AChE) activity were also detected. Apparently, these detoxification methods did not completely protect the spider from the cytotoxicity of Cd stress. Increased mortality of P. pseudoannulata was observed when they were under Cd tress. In total 569 CYP genes belonging to 62 CYP subfamilies were obtained from P. pseudoannulata RNA-seq databases. BlaxtX analysis showed that 150, 161, 11, and 40 CYP genes were similar to the genes dib, phm, sad and shd, respectively, which are thought to catalyze the biosynthesis of ecdysteroids. Gene expression analysis suggested that 25 dib encoding genes, 27 phm encoding genes, 2 sad encoding genes, and 6 shd encoding genes were differentially expressed in TS2 vs. S2 comparison (Cd-treated 2nd instar spider vs. 2nd instar spider), respectively. There were 70 dib, 70 phm and 19 shd encoding genes either upregulated or downregulated, while 3 sad encoding genes were upregulated in TS5 vs. S5 (Cd-treated 5nd instar spider vs. 5nd instar spider). Genes related to heme binding and essential for activating the CYPs were also differentially expressed. Expression levels of cuticle related genes were significant differentially expressed, implying the changes in activities of chitin synthases and chitinase. Therefore we assume that unsuccessful molting process may occur on P. pseudoannulata due to influenced ecdysteroids levels, thus increasing mortality of spider.

语种： 英文

作者： Yang, Huilin;Peng, Yuande;Shi, Yixue;Tian, Jianxiang;Wang, Juan;...

期刊： Environmental Science and Pollution Research,2018年25(24):23875-23882 ISSN：0944-1344

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin; Wang, Zhi; Shi, Yixue; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Changsha 410006, Hunan, Peoples R China.

关键词： Cadmium;Transcriptomic;P. pseudoannulata;Venom glands;Innate immunity

摘要： Due to some similarity of innate immunity between insects and mammals, the study of the molecular mechanism of innate immunity in insects has become a focus of research. However, the exact molecular and cellular basis of immune system in insect remains poorly understood. Characterization of the transcriptomic response to Cd of spider is an effective approach to understanding the innate immunity mechanisms. In this study, we carried out transcriptome sequencing and gene expression analyses to develop molecular resources for Pardosa pseudoannulata venom glands with and without Cd treatments. A total of 92,778 assembled unigenes and 237 Cd stress-associated differentially expressed genes between the Cd-treated and control groups were obtained. Expression profile analysis demonstrated that immunity-related genes involved in bacterial invasion of epithelial cells, leukocyte transendothelial migration, platelet activation, apoptosis, phagosome, and Rap1 signaling pathway were upregulated by Cd exposure, except the genes involved in PPAR signaling pathway were downregulated. Our results provide the first comprehensive transcriptome dataset of venom glands in P. pseudoannulata response to Cd, which is valuable for throws light on the immunotoxicity mechanism of Cd, and the innate immunity complexity. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Zou, Wansheng;Wang, Zhi*;Song, Qisheng;Tang, Shaoxian;Peng, Yuande

期刊： Harmful Algae,2018年77(1):18-28 ISSN：1568-9883

通讯作者： Wang, Zhi

作者机构： [Wang, Zhi; Zou, Wansheng; Tang, Shaoxian] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 NongdaRd, Changsha 410128, Hunan, Peoples R China.;[Zou, Wansheng] Hunan Univ Arts & Sci, Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Hunan, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.;[Peng, Yuande] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Wang, Zhi] Hunan Normal Univ, Sch Life Sci, Changsha 410000, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 NongdaRd, Changsha 410128, Hunan, Peoples R China.

关键词： *Benthic bacteria;*Microcystis aeruginosa;*Recruitment-promoting;*Sediment

摘要： The frequent occurrence of Microcystis aeruginosa blooms benefit from the dormant Microcystis cells, which will be recruited from sediment into overlying water to form a dominant population and algal blooms when external environmental conditions are suitable. Previous studies have unveiled factors involved in M. aeruginosa recruitment and bloom initiation, including nutrition, illumination, temperature, and hydrodynamic force. In this study, three dominant benthic bacterial species isolated from Lake Chongtian with frequent blooms-forming were identified through next generation sequencing (NGS) techniques, and laboratory experiments were conducted on the recruitment of dormant M. aeruginosa cells via co-culture with these bacteria at 10 °C, 15 °C, 20 °C and 25 °C. The results showed that the bacterial strains in sediment proliferated quickly before recruitment of dormant M. aeruginosa cells, subsequently significantly promoted the recruitment of dormant M. aeruginosa via allelochemical (metabolite) production, lower N:P values and lower dissolved oxygen concentrations in the sediment-water interface, and enhanced photosynthesis of M. aeruginosa cells. Furthermore, dormant M. aeruginosa was recruited from sediment at 10 °C when bacterial activity was present, but not recruited when bacterial activity was absent. At 15 °C,20 °Cand 25 °C, there were no remarkable differences in the recruitment rate of dormant M. aeruginosa cells among all bacterial groups, although their recruitment rate were significantly higher than that at 10 °C.These findings suggested that, under laboratory conditions, three benthic bacteria not only had a great influence on promoting the recruitment of dormant M. aeruginosa cells under desirable temperatures, but also can spur recruitment of dormant M. aeruginosacells from sediment at lower temperature (10 °C). © 2018 Elsevier B.V.

语种： 英文

作者： Wang, Juan;Peng, Yuande;Wang, Zhi*;Chai, Xiangping*;Lv, Zhiyue;...

期刊： Biologia,2018年73(11):1099-1108 ISSN：0006-3088

通讯作者： Wang, Zhi;Chai, Xiangping

作者机构： [Wang, Zhi; Wang, Juan] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Wang, Zhi] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Chai, Xiangping] Cent S Univ, Xiangya Hosp 2, Emergency Dept, Changsha 410011, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.

通讯机构： [Wang, Zhi] H;[Wang, Zhi; Chai, Xiangping] C;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;Cent S Univ, Xiangya Hosp 2, Emergency Dept, Changsha 410011, Hunan, Peoples R China.

关键词： Heterometrus liangi;Crude venom;HeLa cells;Cell proliferation;Apoptosis;p21 protein

摘要： Scorpion venoms are important sources for identifying and developing promising biomolecules for cancer treatment. To search for promising biomolecules for cancer research, the effects of crude venom from the scorpion Heterometrus liangi (HLSN) on the proliferation, apoptosis and underline molecular mechanisms of HeLa cells were investigated and compared with that of Buthus martensii (BMK). RT-PCR, Western blot and immunohistochemical staining were used to evaluate the expression levels of p21 in the control and treated cells. The present data showed that when HeLa cells were exposed to HLSN venom for 24 and 48 h, the venom inhibited HeLa cell proliferation in a dose-dependent manner as determined by the methyl thiazolyl tetrazolium (MTT) assay, caused marked morphological changes as revealed by hematoxylin and eosin (HE) staining, scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and induced apoptosis as shown by flow cytometry (FCM). Western blot and RT-PCR analysis revealed that the expression of p21 protein was upregulated at both transcriptional and translational levels, as supported by immunohistochemical staining. Therefore, HLSN crude venom, similar to BMK venom, inhibits proliferation and induces apoptosis in HeLa cells via the induction of p21 expression while it has little adverse effect on non cancer human umbilical vein endothelial (HUVEC-21) cells. © 2018, Institute of Zoology, Slovak Academy of Sciences.

语种： 英文

作者： Yang, Huilin;Wang, Juan;Lv, Zhiyue;Tian, Jianxiang;Peng, Yuande;...

期刊： Ecotoxicology and Environmental Safety,2018年159:1-9 ISSN：0147-6513

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin; Chen, Zhaoyang; Wang, Zhi; Lv, Bo; Sun, Zhiying; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： Cadmium;Intestinal microorganisms;Metatranscriptome;P. pseudoannulata

摘要： Cadmium (Cd) generates a variety of physiological and ecological toxicity to spiders. However, little is known about the effects of Cd on symbiotic bacteria of spiders. Metatranscriptomics is increasing our knowledge of microorganisms in environment. To better understand the impact of Cd on the symbiotic bacteria of spiders, we generated and compared the metatranscriptomes of the intestinal microorganisms of Pardosa pseudoannulata with and without Cd stress. The community structure of intestinal microorganisms in P. pseudoannulata was composed of 4 kingdoms, namely bacteria, viruses, eukaryotes and archaea, including 46 phyla, 97 classes, 184 orders, 339 families, 470 genera, and 598 species. The abundance of eukaryotes, bacteria and viruses was decreased by 0.14%, 1.22% and 2.52% respectively while the archaea was increased by 99.16% when under Cd stress. We identified 1519 differentially expressed genes (DEGs), including 770 up-regulated and 749 down-regulated genes. The results of KEGG annotation revealed that the expression of genes that are involved in the carbon metabolism, protein and amino acid metabolism and synthesis, glucose metabolism, oxidative phosphorylation, and glutathione metabolism were influenced by Cd. Collectively, these findings showed that Cd significantly impacted the community structure and expression of related functional genes of intestinal microorganisms in P. pseudoannulata. © 2018 Elsevier Inc.

语种： 英文

作者： Song, Wei;Jiang, Meijie;Wang, Zongling*;Wang, Hongping;Zhang, Xuelei;...

期刊： 海洋学报(英文版),2018年37(4):102-108 ISSN：0253-505X

通讯作者： Wang, Zongling

作者机构： [Wang, Zongling; Wang, Hongping; Fu, Mingzhu; Jiang, Meijie; Zhang, Xuelei; Song, Wei] State Ocean Adm, Key Lab Sci & Engn Marine Ecol & Environm, Inst Oceanog 1, Qingdao 266061, Peoples R China.;[Wang, Zongling; Wang, Hongping; Fu, Mingzhu; Jiang, Meijie; Zhang, Xuelei; Song, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266235, Peoples R China.;[Song, Wei] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Zongling] S;[Wang, Zongling] Q;State Ocean Adm, Key Lab Sci & Engn Marine Ecol & Environm, Inst Oceanog 1, Qingdao 266061, Peoples R China.;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266235, Peoples R China.

关键词： green tides;source of propagules;Ulva prolifera;anti-fouling

摘要： Since 2007, large-scale green tides dominated by Ulva prolifera consecutively bloomed in the Yellow Sea and caused great economic losses. The fouling U. prolifera on the Pyropia yezoensis aquaculture rafts in the Subei Shoal was regarded as the major source of the floating biomass. However, it was still unclear about the seed source of fouling green macroalgae attached on the rafts. In this study, the field surveys and the indoor experiments were conducted to reveal the source of propagules of the fouling green macroalgae on the rafts and to study the anti-fouling material for P. yezoensis aquaculture rafts which could possibly be a feasible strategy to control the green tides in the Yellow Sea. The results showed that (1) micro-propagules of several green macroalgal species, including U. prolifera, U. linza, U. compressa, U. flexuosa, and Blidingia sp. coexisted in the waters and sediments in the Subei Shoal and their proportion remarkably changed over time; (2) the bamboo poles with peeling treatment could significantly reduce the amount of U. prolifera micro-propagules attached. This study confirmed that the micro-propagules distributed in the Subei Shoal area were the precursors of the green tides, and provided a feasible method to control the Yellow Sea large-scale green tides at the beginning. © 2018, The Chinese Society of Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Yang, Huilin;Peng, Yuande;Tian, Jianxiang;Wang, Juan;Wei, Baoyang;...

期刊： JOURNAL OF ECONOMIC ENTOMOLOGY,2018年111(1):53-64 ISSN：0022-0493

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin; Wang, Zhi; Wei, Baoyang; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： "rice field spider";"study advances";China;"predation function"

摘要： Many laboratory and field studies have been conducted on rice field spiders in China. There are 375 species, 108 genera, and 22 families of rice field spiders distributed within the major rice growing areas and 17 dominant species. The biological and ecological characteristics of 17 rice field spider species have been reported in detail. The biology and ecology of these species show significant differences among regions, farmland habitats, and agricultural practices. Future research should focus on rice field habitat diversity, enhancing the insecticide resistance of dominant spider populations, implementing large-scale breeding of spiders and augmentative release, breeding more leaf dominant species, conducting biosafety assessment of spiders in transgenic crops.

语种： 英文

作者： Liang Yunshan*;Huang Zhijun;Liu Yisong;Qian Ziyan;Yan Biyue;...

期刊： 高等学校化学学报,2018年39(5):883-888 ISSN：0251-0790

通讯作者： Liang Yunshan;Wang Zhi

作者机构： [Yan Biyue; Qian Ziyan; Liang Yunshan; Wang Zhi; Huang Zhijun] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Rural Ecosyst Hlth Dongting La, Changsha 410128, Hunan, Peoples R China.;[Liu Yisong] Hunan Agr Univ, Coll Vet Med, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Liang, YS; Wang, Z] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Rural Ecosyst Hlth Dongting La, Changsha 410128, Hunan, Peoples R China.

关键词： 芘;荧光;表面活性剂;牛血清蛋白;微环境

摘要： 以芘作为外源荧光探针,采用牛血清蛋白作为参照,考察了转基因抗虫蛋白Cry1Ac与4种表面活性剂相互作用的荧光光谱特征.结果表明,鼠李糖脂和Tween 80体系的芘荧光行为有相似的变化规律,可与蛋白质发生稳定的缔合过程.Cry1Ac蛋白的亲水性和分子极性较强,在表面活性剂浓度低时,可导致芘的I1/I3值较高.在阴离子、非离子表面活性剂胶团形成后,2种蛋白质的存在均不改变芘与表面活性剂的结合位点.十六烷基三甲基溴化铵对芘的荧光有一定猝灭作用,且在Cry1Ac蛋白介入下不能形成稳定的胶束.

语种： 中文

作者： 唐美蓉;王娟;卓俊哲;黄婷;王智

期刊： 经济动物学报,2018年22(01):1-5 ISSN：1007-7448

作者机构： 湖南农业大学生物科学技术学院,长沙,410128;[唐美蓉; 王娟; 黄婷; 王智; 卓俊哲] 湖南农业大学

关键词： 梁氏异蝎;毒素;HeLa细胞;细胞增殖;形态学

摘要： 采用倒置相差显微镜观察法、HE染色法、扫描电镜法和透射电镜法对梁氏异蝎毒素抑制He La细胞增殖的形态学影响进行了较系统的研究。用不同浓度的蝎毒处理He La细胞（0,20,40,60μg/m L）,发现随着蝎毒浓度的升高He La细胞的死亡率增加,经毒素作用后,试验组与对照组相比,细胞突起收缩、形态变圆、体积缩小,染色质浓缩,导致细胞大量凋亡。结果表明：梁氏异蝎毒素对He La细胞的形态学具有很大的影响,能显著抑制He La细胞增殖。该研究对利用梁氏异蝎毒素开发新型抗癌药物提供了新的信息。

语种： 中文

作者： Peng, Shu-guang;Pang, Yi-lin;Zhu, Qi;Kang, Jing-he;Liu, Ming-xin;...

期刊： BioMed Research International,2018年2018:8594767 ISSN：2314-6133

通讯作者： Wang, Zheng

作者机构： [Liu, Ming-xin; Wang, Zheng; Pang, Yi-lin; Peng, Shu-guang; Zhu, Qi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Peng, Shu-guang] Res Inst Hunan Tobacco Sci, Changsha 410007, Hunan, Peoples R China.;[Peng, Shu-guang] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.;[Kang, Jing-he] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Wang, Zheng] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

摘要： Rosiglitazone (RG) is a well-known activator of peroxisome proliferator-activated receptor-gamma (PPARγ) and used to treat hyperglycemia and type 2 diabetes; however, its clinical application has been confounded by adverse side effects. Here, we assessed the roles of chlorogenic acid (CGA), a phenolic secondary metabolite found in many fruits and vegetables, on the differentiation and lipolysis of mouse 3T3-L1 preadipocytes. The results showed that CGA promoted differentiation in vitro according to oil red O staining and quantitative polymerase chain reaction assays. As a potential molecular mechanism, CGA downregulated mRNA levels of the adipocyte differentiation-inhibitor gene Pref1 and upregulated those of major adipogenic transcriptional factors (Cebpb and Srebp1). Additionally, CGA upregulated the expression of the differentiation-related transcriptional factor PPARγ2 at both the mRNA and protein levels. However, following CGA intervention, the accumulation of intracellular triacylglycerides following preadipocyte differentiation was significantly lower than that in the RG group. Consistent with this, our data indicated that CGA treatment significantly upregulated the expression of lipogenic pathway-related genes Plin and Srebp1 during the differentiation stage, although the influence of CGA was weaker than that of RG. Notably, CGA upregulated the expression of the lipolysis-related gene Hsl, whereas it did not increase the expression of the lipid synthesis-related gene Dgat1. These results demonstrated that CGA might function as a potential PPARγ agonist similar to RG; however, the impact of CGA on lipolysis in 3T3-L1 preadipocytes differed from that of RG. © 2018 Shu-guang Peng et al.

语种： 英文

作者： Yang, Huilin;Peng, Yuande;Shi, Yixue;Tian, Jianxiang;Wang, Juan;...

期刊： Ecotoxicology,2018年27(2):198-208 ISSN：0963-9292

通讯作者： Wang, Zhi;Lv, Zhiyue

作者机构： [Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin; Wang, Zhi; Shi, Yixue; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Xianjin; Xu, Xiang] Hunan Normal Univ, Coll Life Sci, Changsha 410006, Hunan, Peoples R China.

通讯机构： [Wang, Zhi] H;[Lv, Zhiyue] S;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.

关键词： Cadmium;Transcriptomic;P. pseudoannulata;Cerebral ganglion

摘要： Cadmium (Cd) is a heavy metal that can cause irreversible toxicity to animals, and is an environmental pollutant in farmlands. Spiders are considered to be an excellent model for investigating the impacts of heavy metals on the environment. To date, the changes at the molecular level in the cerebral ganglia of spiders are poorly understood. Cd exposure leads to strong damage in the nervous system, such as apoptosis and necrosis of nerve cells, therefore we conducted a transcriptomic analysis of Pardosa pseudoannulata cerebral ganglia under Cd stress to profile differential gene expression (DGE). We obtained a total of 123,328 assembled unigenes, and 1441 Cd stress-associated DEGs between the Cd-treated and control groups. Expression profile analysis demonstrated that many genes involved in calcium signaling, cGMP—PKG signaling, tyrosine metabolism, phototransduction–fly, melanogenesis and isoquinoline alkaloid biosynthesis were up-regulated under Cd stress, whereas oxidative phosphorylation-related, nervous disease-associated, non-alcoholic fatty liver disease-associated, and ribosomal-associated genes were down-regulated. Here, we provide a comprehensive set of DEGs influenced by Cd stress, and heavy metal stress, and provide new information for elucidating the neurotoxic mechanisms of Cd stress in spiders. © 2018, The Author(s).

语种： 英文

作者： Wang, Yan;Fei, Yanquan;Liu, Lirui;Xiao, Yunhua;Pang, Yilin;...

期刊： International Journal of Molecular Sciences,2018年19(11) ISSN：1422-0067

通讯作者： Wang, Zheng;Kang, Jinhe

作者机构： [Fei, Yanquan; Pang, Yilin; Wang, Zheng; Xiao, Yunhua; Wang, Yan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Wang, Yan] Hunan Univ Humanities Sci & Technol, Coll Agr & Biotechnol, Loudi 417000, Peoples R China.;[Wang, Yan; Kang, Jinhe] Chinese Acad Sci, Inst Subtrop Agr,Minist Agr, CAS Key Lab Agroecol Proc Subtrop Reg,South Cent, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.;[Liu, Lirui] Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China.

通讯机构： [Wang, Zheng] H;[Kang, Jinhe] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Inst Subtrop Agr,Minist Agr, CAS Key Lab Agroecol Proc Subtrop Reg,South Cent, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.

关键词： Polygonatum odoratum;gut microbiota;high-fat diet;obesity;polysaccharides

摘要： Increasing evidence suggests that the gut microbiota plays vital roles in metabolic diseases. Polygonatum odoratum extract alleviates hyperglycemia and hyperlipidemia, but the underlying mechanism remains unclear. This study investigated the effects of P. odoratum polysaccharides (POPs) on high-fat diet (HFD)-induced obesity in rats and whether these effects were related to modulation of gut microbiota. POP treatment attenuated weight gain, fat accumulation, epididymal adipocyte size, liver triglycerides, and total liver cholesterol content in HFD-fed rats. POP administration also increased short-chain fatty acids (SCFAs), including isobutyric acid, butyric acid, and valeric acid. POP upregulated the expression of genes involved in adipocyte differentiation (Pparg, Cebpa, Cebpb) and lipolysis (Ppara, Atgl), and downregulated those related to lipid synthesis (Srebpf1, Fabp4, Fas), with corresponding changes in PPARγ and FABP4 protein expression. Finally, POP enhanced species richness and improved the gut microbiota community structure, reducing the relative abundances of Clostridium, Enterococcus, Coprobacillus, Lactococcus, and Sutterella. Principal coordinates analysis (PCoA) revealed a clear separation between HFD-fed rats and all other treatment groups. Correlation analysis identified negative and positive associations between obesity phenotypes and 28 POP-influenced operational taxonomic units (OTUs), including putative SCFA-producing bacteria. Our data suggest that POP supplementation may attenuate features of obesity in HFD-fed rats in association with the modulation of gut microbiota.

语种： 英文

作者： Wang, Juan;Wei, Baoyang;Peng, Yuande;Huang, Ting;Yang, Huilin;...

期刊： Environmental Science and Pollution Research,2018年25(34):34294-34305 ISSN：0944-1344

通讯作者： Wang, Zhi;Lv, Zhiyue

作者机构： [Peng, Xianjin; Wang, Zhi; Wang, Juan; Xu, Xiang] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;[Yang, Huilin; Wang, Zhi; Wei, Baoyang; Sun, Zhiying; Huang, Ting; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande; Xie, Chunliang] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Lv, Zhiyue] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;[Lv, Zhiyue] S;Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou 510080, Guangdong, Peoples R China.

关键词： P. pseudoannulata;Cadmium;Detoxification;Immunity;Development

摘要： Cadmium (Cd) can be transferred and accumulated in spiders, posing a survival risk to them. To analyze potential biological damage caused by Cd accumulation and relevant detoxification strategies employed by spiders in response to Cd exposure, we conducted transcriptome analysis of the 5th instar spider P. pseudoannulata, a common spider species playing a vital role in natural pest control in agricultural fields of southern China. We obtained 92,778 unigenes with an average length of 1104 bp and identified 302, 655, and 424 differentially expressed genes (DEGs) in the spiders fed with Cd-containing fruit flies for 2, 5, and 8 days, respectively. Results showed that the body mass of Cd-containing P. pseudoannulata were reduced when compared with controls, presumably due to delayed maturation of tissues and organs. Meanwhile, functional analysis of DEGs indicated that Cd may have a negative effect on neural signal transduction and molt cycle of the spider. For defense strategies, detoxification enzymes like glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and P450, and typical proteins like heat shock protein and metallothionein were all differentially expressed in response to Cd stress. Besides, innate immune responses like toll-like receptor signaling pathways were also upregulated. Multiple critical Cd-responsive genes involved in biological damage, detoxification, and immune response were identified, providing referable foundation for further research on Cd toxicity to P. pseudoannulata. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： 熊慧;黎丹;梁国辉;王智;魏宝阳

期刊： 湖南农业大学学报(自然科学版),2018年44(4):430-434,446 ISSN：1007-1032

作者机构： [熊慧; 黎丹; 梁国辉; 王智; 魏宝阳] 湖南农业大学生物科学技术学院, 湖南, 长沙, 410128

关键词： 拟环纹豹蛛;Bt蛋白;肠道细菌;多样性

摘要： 采用16S r DNA分子生物学分析方法,分别对试验组（喂食含Cry1Ab蛋白培养基上饲养的果蝇）和对照组（喂食普通培养基上饲养的果蝇）的拟环纹豹雌、雄蛛肠道细菌进行分析。发现,拟环纹豹蛛肠道细菌丰富,雌蛛肠道细菌丰富度大于雄蛛肠道细菌丰富度;优势菌属分别是短杆菌属（Brevibacterium）、短波单胞菌属（Brevundimonas）、葡萄球菌属（Staphylococcus）、乳杆菌属（Lactobacillus）、丛毛单胞菌属（Comamonas）、德沃斯氏菌属（Devosia）、希瓦氏菌属（Shewanella）、Leucobacter、杆菌属（Brachybacterium）、梭菌属（Clostridium）;Bt蛋白对拟环纹豹蛛肠道细菌多样性有一定的影响,对放线菌属（Actinomycetes）的影响最小,对乳杆菌属（Lactobacillus）和梭菌属（Clostridium）的影响较大。

语种： 中文

作者： 邹万生;王智;宋齐生;罗玉双;刘良国;...

期刊： 环境科学学报,2017年37(11):4160-4169 ISSN：0253-2468

通讯作者： Wang, Z.

作者机构： 湖南农业大学生物科学与技术学院, 水产高校健康生产湖南省协同创新中心;[邹万生] 与动物学湖南省高校重点实验室, 长沙, 410128;湖南农业大学生物科学与技术学院, 长沙, 410128;Division of Plant Sciences,University of Missouri, USA, Columbia, 65211;湖南文理学院生命与环境科学学院, 水产高校健康生产湖南省协同创新中心

关键词： 底泥菌;铜绿微囊藻;休眠体;复苏;调控作用

摘要： 将起始浓度不同的单菌和混合菌(Ba.sp06+Str.v13)分别与铜绿微囊藻休眠体及底泥共包埋,在梯度温度下进行24 d休眠体复苏实验.结果表明,温度低于10 ℃时,菌株Str.v13的生长增殖能力显著强于菌株Ba.sp06,各菌组休眠体复苏率最高为0.02%;在温度为15和20 ℃条件下,Ba.sp06与Str.v13两菌株浓度比例(以下简称“菌比”)为1∶1时混合菌组休眠体复苏率(15 ℃时为15.5%,20 ℃时为23.5%)与对照组无显著差异(15 ℃时为15%,20 ℃时为22.6%),但显著低于Ba.sp06单菌组(34.5%);菌比2∶1时Ba.sp06菌对Str.v13菌具抑制效应,混合菌组复苏率(15 ℃时为31%, 20 ℃时为35%)显著高于对照组,而与Ba.sp06单菌组无显著差异;菌比为1∶2时Str.v13菌对Ba.sp06菌具抑制效应,混合菌组休眠体复苏率(15 ℃时为6%,20 ℃时为6.5%)均显著低于菌比为1∶1、2∶1时及Ba.sp06单菌组,但高于Str.v13单菌组(3%).同时,各复苏阶段对复苏率的贡献主要集中在启动复苏的第12~ 18 d.实验表明,底泥表层铜绿微囊藻休眠体复苏不仅受温度影响(>10 ℃),且复苏率受底栖菌Ba.sp06与Str.v13的起始浓度影响,Ba.sp06菌的起始浓度高于Str.v13菌时促进休眠体复苏,低于Str.v13菌时复苏受到抑制.因此,可以在铜绿微囊藻休眠体复苏前期通过调节Ba.sp06与Str.v13菌比来调控休眠体复苏率,为从源头防控微囊藻水华暴发提供理论依据.

语种： 中文

作者： 邹万生;王智;刘良国;王文彬;石迎普

期刊： 生态学报,2017年37(19):6597-6606 ISSN：1000-0933

通讯作者： Wang, Z.

作者机构： 湖南农业大学生物科学与技术学院, 水产高校健康生产湖南省协同创新中心;[邹万生] 动物学湖南省高校重点实验室, 长沙, 410218;湖南农业大学生物科学与技术学院, 长沙, 410218;湖南文理学院生命科学学院, 水产高校健康生产湖南省协同创新中心;[刘良国; 王文彬] 动物学湖南省高校重点实验室, 常德, 415000

关键词： 微囊藻;休眠体;优势菌群;复苏;底泥;上覆水

摘要： 对频繁暴发微囊藻水华的西洞庭冲天湖表层底泥和上覆水取样,检测和分析了底泥表层微囊藻休眠体丰度和菌浓度、上覆水中微囊藻细胞丰度和菌浓度以及部分理化性质,结合室内模拟试验。结果表明:2—6月份冲天湖底泥表层和上覆水中总菌浓度均显著上升(P<0.05),底泥表层总菌浓度显著高于上覆水(P<0.05),优势菌群均为微小杆菌属(Exiguobacterium)、假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus);4月份底泥表层微囊藻休眠体开始复苏且休眠体丰度下降,6月份休眠体丰度显著低于4—5月份(P<0.05),而上覆水中微囊藻细胞丰度上升,6月份显著高于4—5月份(P<0.05);复苏优势藻为铜绿微囊藻(Microcystis aeruginosa)、水华微囊藻(Microcystis flos-aqua)和惠氏微囊藻(Microcystis wesenbergii);复苏期间促休眠体复苏优势菌群浓度显著上升、“底泥-上覆水”界面溶解氧浓度与TN/TP比显著下降(P<0.05)。说明冲天湖底泥表层和上覆水优势菌群可能通过改变底泥表层理化环境影响微囊藻休眠体复苏。

语种： 中文

作者： Yang, Huilin;Peng, Yuande;Tian, Jianxiang;Wang, Juan;Hu, Jilin;...

期刊： Plant Cell Reports,2017年36(4):505-517 ISSN：0721-7714

通讯作者： Wang, Zhi

作者机构： [Yang, Huilin; Wang, Zhi; Hu, Jilin; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Yang, Huilin] Hunan Agr Univ, Coll Orient Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Peng, Yuande] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Tian, Jianxiang] Hunan Agr Univ, Coll Continuing Educ, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.

关键词： Spiders;Bt rice;Non-target effects;GM crops;Spider ecology

摘要： Since the birth of transgenic crops expressing Bacillus thuringiensis (Bt) toxin for pest control, the public debate regarding ecological and environmental risks as well as benefits of Bt crops has continued unabated. The impact of Bt crops, especially on non-target invertebrates, has received particular attention. In this review, we summarize and analyze evidences for non-target effects of Bt rice on spiders, major predators in rice fields. Bt rice has been genetically modified to express the Bt protein, which has been shown to be transferred and accumulate in spiders as part of their food chain. Moreover, the Bt protein exhibits unintended effects on the physiology of spiders and spreads to higher trophic levels. Spiders possess unique physiological and ecological characteristics, revealing traits of surrogate species, and are thus considered to be excellent non-target arthropod model systems for study of Bt protein impacts. Due to the complexities of Bt protein transfer and accumulation mechanisms, as well as the apparent lack of information about resulting physiological, biochemical, and ecological effects on spiders, we raise questions and provide recommendations for promising further research.

语种： 英文

作者： Li, Rong;Yan, Zhenzhen;Wang, Juan;Song, Qisheng;Wang, Zhi*

期刊： BMC Biotechnology,2017年17(1):1-11 ISSN：1472-6750

通讯作者： Wang, Zhi

作者机构： [Li, Rong; Wang, Zhi; Yan, Zhenzhen; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Li, Rong] Hunan Univ Arts & Sci, Dept Biosci, Changde 415000, Peoples R China.;[Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.

通讯机构： [Wang, Zhi] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

关键词： The venom apparatus;Transcriptome;Pardosa pseudoannulata;Bt protein;Immune system

摘要： Background: Pardosa pseudoannulata is a prevailing spider species, and has been regarded as an important bio-control agent of insect pests in farmland of China. However, the available genomic and transcriptomic databases of P. pseudoannulata and their venom are limited, which severely hampers functional genomic analysis of P. pseudoannulata. Recently high-throughput sequencing technology has been proved to be an efficient tool for profiling the transcriptome of relevant non-target organisms exposed to Bacillus thuringiensis (Bt) protein through food webs. Results: In this study, the transcriptome of the venom apparatus was analyzed. A total of 113,358 non-redundant unigenes were yielded, among which 34,041 unigenes with complete or various length encoding regions were assigned biological function annotations and annotated with gene ontology and karyotic orthologous group terms. In addition, 3726 unigenes involved in response to stimulus and 720 unigenes associated with immune-response pathways were identified. Furthermore, we investigated transcriptomic changes in the venom apparatus using tag-based DGE technique. A total of 1724 differentially expressed genes (DEGs) were detected, while 75 and 372 DEGs were functionally annotated with KEGG pathways and GO terms, respectively. qPCR analyses were performed to verify the DEGs directly or indirectly related to immune and stress responses, including genes encoding heat shock protein, toll-like receptor, GST and NADH dehydrogenase. Conclusion: This is the first study conducted to specifically investigate the venom apparatus of P. pseudoannulata in response to Bt protein exposure through tritrophic chain. A substantial fraction of transcript sequences was generated by high-throughput sequencing of the venom apparatus of P. pseudoannulata. Then a comparative transcriptome analysis showing a large number of candidate genes involved in immune response were identified by the tag-based DGE technology. This transcriptome dataset will provide a comprehensive sequence resource for furture molecular genetic research of the venom apparatus of P. pseudoannulata. © 2017 The Author(s).

语种： 英文