作者： Luo, Zhoufei;Xu, Mengwei;Wang, Ruozhong;Liu, Xiubing;Huang, Yongkang;...

期刊： Phytochemistry,2021年183:112611 ISSN：0031-9422

通讯作者： Xiao, Langtao

作者机构： [Luo, Zhoufei; Wang, Ruozhong; Xu, Mengwei; Xiao, Langtao] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Huang, Yongkang; Luo, Zhoufei; Wang, Ruozhong; Xu, Mengwei; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Peoples R China.;[Liu, Xiubing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.

通讯机构： [Langtao Xiao] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, China

关键词： Magnetic solid phase extraction;In situ derivatization;Oilseeds;Phytohormones;Ultra-high performance liquid;chromatography-tandem mass spectrometry

摘要： Magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones in a single rapeseed seed was developed by using ultra-high performance liquid chromatography-tandem mass spectrometry. The Fe3O4@Ti3C2@beta-cyclodextrin nanoparticles were firstly synthesized and used as an adsorbent for the solid-phase extraction of phytohormones. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Satisfactory methodological performance was achieved by optimization of the parameters. Linearities (R-2 > 0.9928) and recoveries (80.4 %-115.1%) at three spiked levels, as well as the low matrix effect (from -16.63% to 17.06%) and limits of detection (0.89-13.62 pg/mL) were obtained. The spatio-temporal profiling of target phytohormones in different tissues of rapeseed germination was investigated. This method was successfully employed for analyzing target phytohormones in different oilseeds samples.

语种： 英文

作者： Chen, Lu;Huang, Xu-Xu;Zhao, Shu-Man;Xiao, Dong-Wang;Xiao, Lang-Tao;...

期刊： Proceedings of the National Academy of Sciences of the United States of America,2020年117(12):6910-6917 ISSN：0027-8424

通讯作者： Hou, Bing-Kai

作者机构： [Huang, Xu-Xu; Wang, Wen-Shuai; Chen, Lu; Xiao, Dong-Wang; Zhao, Shu-Man; Ding, Zhaojun; Hou, Bing-Kai; Li, Yan-Jie] Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Dev & Environm Adaptat Biol, Qingdao 266237, Peoples R China.;[Tong, Jian-Hua; Xiao, Lang-Tao] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.

通讯机构： [Hou, Bing-Kai] S;Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Dev & Environm Adaptat Biol, Qingdao 266237, Peoples R China.

关键词： Arabidopsis thaliana;auxin;glycosylation

摘要： Auxin is a class of plant hormone that plays a crucial role in the life cycle of plants, particularly in the growth response of plants to ever-changing environments. Since the auxin responses are concentration-dependent and higher auxin concentrations might often be inhibitory, the optimal endogenous auxin level must be closely controlled. However, the underlying mechanism governing auxin homeostasis remains largely unknown. In this study, a UDP-glycosyltransferase (UGT76F1) was identified from Arabidopsis thaliana, which participates in the regulation of auxin homeostasis by glucosylation of indole-3-pyruvic acid (IPyA), a major precursor of the auxin indole-3-acetic acid (IAA) biosynthesis, in the formation of IPyA glucose conjugates (IPyA-Glc). In addition, UGT76F1 was found to mediate hypocotyl growth by modulating active auxin levels in a light- and temperature-dependent manner. Moreover, the transcription of UGT76F1 was demonstrated to be directly and negatively regulated by PIF4, which is a key integrator of both light and temperature signaling pathways. This study sheds a light on the trade-off between IAA biosynthesis and IPyA-Glc formation in controlling auxin levels and reveals a regulatory mechanism for plant growth adaptation to environmental changes through glucosylation of IPyA. © 2020 National Academy of Sciences. All rights reserved.

语种： 英文

作者： Zhang, Xiao-Fan;Tong, Jian-Hua;Bai, Ai-Ning;Liu, Chun-Ming;Xiao, Lang-Tao;...

期刊： 植物学报：英文版,2020年62(10):1625-1637 ISSN：1672-9072

通讯作者： Xue, Hong-Wei

作者机构： [Zhang, Xiao-Fan] Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China.;[Tong, Jian-Hua; Xiao, Lang-Tao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.;[Bai, Ai-Ning; Liu, Chun-Ming] Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China.;[Liu, Chun-Ming] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China.;[Xue, Hong-Wei] Shanghai Jiao Tong Univ, Joint Ctr Single Cell Biol, Sch Agr & Biol, Shanghai 200240, Peoples R China.

通讯机构： [Xue, Hong-Wei] S;[Xue, Hong-Wei] C;Shanghai Jiao Tong Univ, Joint Ctr Single Cell Biol, Sch Agr & Biol, Shanghai 200240, Peoples R China.;Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China.

关键词： FIR;GRAIN;SHAPE

摘要： Hormones are important signaling molecules regulating developmental processes and responses to environmental stimuli in higher plants. Rice endosperm, the portion of the seed surrounding the embryo, is the main determinant of rice grain shape and yield; however, the dynamics and exact functions of phytohormones in developing endosperm remain elusive. Through a systemic study including transcriptome analysis, hormone measurement, and transgene-based endosperm-specific expression of phytohormone biosynthetic enzymes, we demonstrated that dynamic phytohormone levels play crucial roles in the developing rice endosperm, particularly in regard to grain shape and quality. We detected diverse, differential, and dramatically changing expression patterns of genes related to hormone biosynthesis and signaling during endosperm development, especially at early developmental stages. Liquid chromatography measurements confirmed the dynamic accumulation of hormones in developing endosperm. Further transgenic analysis performed on plants expressing hormone biosynthesis genes driven by an endosperm-specific promoter revealed differential effects of the hormones, especially auxin and brassinosteroids, in regulating grain shape and quality. Our studies help elucidate the distinct roles of hormones in developing endosperm and provide novel and useful tools for influencing crop seed shape and yield. © 2020 Institute of Botany, Chinese Academy of Sciences

语种： 英文

作者： Lyu, Guizhen;Li, Dongbing;Xiong, Hui;Xiao, Langtao;Tong, Jianhua;...

期刊： International Journal of Molecular Sciences,2020年21(7):2496 ISSN：1661-6596

通讯作者： Li, Shaoshan

作者机构： [Ning, Chanjuan; Lyu, Guizhen; Wang, Ping; Xiong, Hui; Li, Dongbing; Li, Shaoshan] South China Normal Univ, Sch Life Sci, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangzhou 510631, Peoples R China.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones, Changsha 410128, Peoples R China.

通讯机构： [Li, Shaoshan] S;South China Normal Univ, Sch Life Sci, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangzhou 510631, Peoples R China.

关键词： bbx24 protein;ELAV like protein 2;flavonoid;protein;sto protein;unclassified drug;acyltransferase;Arabidopsis protein;AT5G13930 protein, Arabidopsis;STO protein, Arabidopsis;Article;cation exchange;cell membrane;chloroplast membrane;comparative study;controlled study;DNA responsive element;iTRAQ labeling;light absorption;nonhuman;nonphotochemical quenching;photosynthesis;photosynthetic phosphorylation;promoter region;protein analysis;protein expression;protein interaction;protein transport;proteomics;quantitative analysis;real time polymerase chain reaction;signal transduction;ultraviolet B radiation;upregulation;adverse event;Arabidopsis;biosynthesis;drug effect;gene expression regulation;genetics;growth, development and aging;metabolism;mutation;procedures;proteomics;radiation response;ultraviolet radiation;Acyltransferases;Arabidopsis;Arabidopsis Proteins;Biosynthetic Pathways;Gene Expression Regulation, Developmental;Gene Expression Regulation, Plant;Mutation;Photosynthesis;Proteomics;Ultraviolet Rays

摘要： Plants use solar radiation for photosynthesis and are inevitably exposed to UV-B. To adapt to UV-B radiation, plants have evolved a sophisticated strategy, but the mechanism is not well understood.We have previously reported that STO (salt tolerance)/BBX24 is a negative regulator of UV-B-induced photomorphogenesis. However, there is limited knowledge of the regulatory network of STO in UV-B signaling. Here, we report the identification of proteins differentially expressed in the wild type (WT) and sto mutant after UV-B radiation by iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomic analysis to explore differential proteins that depend on STO and UV-B signaling. A total of 8212 proteins were successfully identified, 221 of them were STO-dependent proteins in UV-B irradiated plants. The abundances of STO-dependent PSB and LHC (light-harvesting complex) proteins in sto mutants decreased under UV-B radiation, suggesting that STO is necessary to maintain the normal accumulation of photosynthetic system complex under UV-B radiation to facilitate photosynthesis photon capture. The abundance of phenylalanine lyase-1 (PAL1), chalcone synthetase (CHS), and flavonoid synthetase (FLS) increased significantly after UV-B irradiation, suggesting that the accumulation of flavonoids do not require STO, but UV-B is needed. Under UV-B radiation, STO stabilizes the structure of antenna protein complex by maintaining the accumulation of PSBs and LHCs, thereby enhancing the non-photochemical quenching (NPQ) ability, releasing extra energy, protecting photosynthesis, and ultimately promoting the elongation of hypocotyl. The accumulation of flavonoid synthesis key proteins is independent of STO under UV-B radiation. Overall, our results provide a comprehensive regulatory network of STO in UV-B signaling. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Yu, Le;Gao, Bin;Li, Yelin;Tan, Weijian;Li, Mingkang;...

期刊： Journal of Plant Physiology,2020年246-247:153139 ISSN：0176-1617

通讯作者： Liu, Yonghai

作者机构： [Liu, Yonghai; Zhou, Liping; Li, Mingkang; Tan, Weijian; Gao, Bin; Li, Yelin; Yu, Le] Zhaoqing Univ, Coll Life Sci, Zhaoqing 526061, Guangdong, Peoples R China.;[Peng, Changlian] South China Normal Univ, Coll Life Sci, Guangzhou 510631, Peoples R China.;[Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Liu, Yonghai] Z;Zhaoqing Univ, Coll Life Sci, Zhaoqing 526061, Guangdong, Peoples R China.

关键词： antioxidant;ascorbic acid;fused heterocyclic rings;galactonolactone dehydrogenase;GR24 strigolactone;hydrogen peroxide;lactone;oxidoreductase;enzymology;gene expression regulation;genetics;metabolism;Oryza;transgenic plant;Antioxidants;Ascorbic Acid;Gene Expression Regulation, Plant;Heterocyclic Compounds, 3-Ring;Hydrogen Peroxide;Lactones;Oryza;Oxidoreductases Acting on CH-CH Group Donors;Plants, Genetically Modified

摘要： Rice tillering, which determines the panicle number per plant, is an important agronomic trait for grain production. In higher plants, ascorbic acid (Asc) plays a major role in ROS-scavenging activity. L-Galactono-1, 4-lactone dehydrogenase (GalLDH, EC1.3.2.3) is an enzyme that catalyzes the last step of Asc biosynthesis in plants. Previously, we have reported that homozygous L-GalLDH-suppressed transgenic rice plants (GI) display a reduced tiller number and a lower level of foliar carotenoids (Car) compared with wild type. Strigolactones (SL), which play an important role in the suppression of shoot branching, are synthesized in the roots of rice plant using Car as substrates. In this paper, the relationship between Asc, SL, the accumulation of H2O2, changes in antioxidant capacity, enzyme activities, and gene transcriptions related to the synthesis of SL were analyzed in transgenic rice plants for L-GalLDH suppressed (GI-1 and GI-2) and overexpressing (GO-2). The results showed that the altered level of Asc in the L-GalLDH transgenic rice plants leads to a change in redox homeostasis, resulting in a marked accumulation of H2O2 and decreased antioxidant capacity in GI-1 and GI-2, but lower H2O2 content and increased antioxidant capacity in GO-2. Meanwhile, the altered level of Asc also leads to altered enzyme activities and gene transcript abundances related to SL synthesis in L-GalLDH transgenics. These observations support the conclusion that Asc influences tiller number in the L-GalLDH transgenics by affecting H2O2 accumulation and antioxidant capacity, and altering those enzyme activities and gene transcript abundances related to SL synthesis. © 2020 Elsevier GmbH

语种： 英文

作者： Huang, Chao;David, Molly P.;Cao, Zhi-Lin;Xiao, Lang-Tao*

期刊： Plant Growth Regulation,2020年91(2):209-219 ISSN：0167-6903

通讯作者： Xiao, Lang-Tao

作者机构： [Huang, Chao; Xiao, Lang-Tao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.;[David, Molly P.] Chongqing Med Univ, Dept Basic Med, Chongqing 400016, Peoples R China.;[Cao, Zhi-Lin] Henan Univ Engn, Dept Resources & Environm, Zhengzhou 451191, Peoples R China.

通讯机构： [Xiao, Lang-Tao] H;Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.

关键词： Arabidopsis thaliana;CHLM;reactive oxygen species;salt stress;nuclear gene expression

摘要： Mg-protoporphyrin IX methyltransferase (CHLM) plays a crucial role in chlorophyll biosynthesis and converts Mg-protoporphyrin IX to Mg-protoporphyrin IX monomethyl ester. Previous research has indicated that down-expression of CHLM causes a pale green phenotype and Mg-protoporphyrin IX accumulation in Arabidopsis thaliana plants. Here, we report that a novel missense mutation (G to A transition) in the CHLM gene, results in the Gly59 → Glu amino acid substitution in the corresponding protein of Arabidopsis chlm-4 mutant plants. In silico modelling studies suggested that this substitution may disrupt membrane association of CHLM. Functional studies showed that the mutant form of CHLM leads to the excessive accumulation of superoxide anion radicals (O2−), although elimination of O2− could alleviate the yellow phenotype observed in chlm-4. In addition, qRT-PCR and western blotting analysis demonstrated that the expression of O2− scavengers, FSD1 and CSD2, were repressed in chlm-4 plants. The accumulation of photosystem II proteins was also decreased due to the CHLM mutation. Furthermore, we found that chlm-4 plants exhibited hypersensitive salt-stress during seed germination and down-expression of the salt stress-responsive CBFs-family genes. These results suggest CHLM positively regulates the expression of multiple photosynthetic-related and stress-responsive nuclear genes. © 2020, Springer Nature B.V.

语种： 英文

作者： Zhang, Xue;Liu, Lu;Wang, Hongfeng;Gu, Zhiqun;Liu, Yafei;...

期刊： International Journal of Molecular Sciences,2020年21(22):8742 ISSN：1661-6596

通讯作者： Zhou, Chuanen

作者机构： [Zhou, Chuanen; Gu, Zhiqun; Wang, Minmin; Zhang, Xue; Xu, Yiteng; Wang, Min; Liu, Lu; Liu, Yafei] Shandong Univ, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Sch Life Sci, Qingdao 266237, Peoples R China.;[Wang, Hongfeng] Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Peoples R China.;[Li, Gang; Shi, Qingbiao] Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones, Changsha 410128, Peoples R China.;[Wang, Zeng-Yu] Qingdao Agr Univ, Grassland Agrihusb Res Ctr, Qingdao 266109, Peoples R China.

通讯机构： [Zhou, Chuanen] S;Shandong Univ, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Sch Life Sci, Qingdao 266237, Peoples R China.

关键词： auxin;protein derivative;protein mtpin1;protein mtpin3;unclassified drug;Arabidopsis protein;carrier protein;chlorophyll;indoleacetic acid derivative;isoprotein;phytohormone;PIN1 protein, Arabidopsis;PIN3 protein, Arabidopsis;Article;barrel medic;cell size;chlorophyll content;controlled study;leaf development;nonhuman;orthology;petiole;phenotype;phylogeny;plant leaf;plant response;plant stress;protein function;regulatory mechanism;shade;transcriptomics;Arabidopsis;barrel medic;biosynthesis;conserved sequence;gene expression regulation;genetics;growth, development and aging;hypocotyl;light;metabolism;mutation;photosynthesis;radiation response;Arabidopsis;Arabidopsis Proteins;Chlorophyll;Conserved Sequence;Gene Expression Regulation, Developmental;Gene Expression Regulation, Plant;Hypocotyl;Indoleacetic Acids;Light;Medicago truncatula;Membrane Transport Proteins;Mutation;Photosynthesis;Plant Growth Regulators;Plant Leaves;Protein Isoforms

摘要： Polar auxin transport mediated by PIN-FORMED (PIN) proteins is critical for plant growth and development. As an environmental cue, shade stimulates hypocotyls, petiole, and stem elongation by inducing auxin synthesis and asymmetric distributions, which is modulated by PIN3,4,7 in Arabidopsis. Here, we characterize the MtPIN1 and MtPIN3, which are the orthologs of PIN3,4,7, in model legume species Medicago truncatula. Under the low Red:Far-Red (R:FR) ratio light, the expression of MtPIN1 and MtPIN3 is induced, and shadeavoidance response is disrupted in mtpin1 mtpin3 double mutant, indicating that MtPIN1 and MtPIN3 have a conserved function in shade response. Surprisingly, under the normal growth condition, mtpin1 mtpin3 displayed the constitutive shade avoidance responses, such as the elongated petiole, smaller leaf, and increased auxin and chlorophyll content. Therefore, MtPIN1 and MtPIN3 play dual roles in regulation of shadeavoidance response under different environments. Furthermore, these data suggest that PIN3,4,7 and its orthologs have evolved conserved and specific functions among species. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Chen, Huhui;Ruan, Jiuxiao;Chu, Pu*;Fu, Wei;Liang, Zhenwei;...

期刊： PLANT JOURNAL,2020年101(2):310-323 ISSN：0960-7412

通讯作者： Huang, Shangzhi;Chu, Pu

作者机构： [Chen, Huhui; Li, Chenlong; Liang, Zhenwei; Huang, Shangzhi; Ruan, Jiuxiao; Fu, Wei; Li, Yin] Sun YAT SEN Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China.;[Chen, Huhui; Li, Chenlong; Liang, Zhenwei; Huang, Shangzhi; Ruan, Jiuxiao; Fu, Wei; Li, Yin] Sun YAT SEN Univ, Sch Life Sci, Guangdong Key Lab Plant Resource, Guangzhou 510275, Guangdong, Peoples R China.;[Chu, Pu] Nanjing Agr Univ, Coll Agr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.;[Liu, Jun] Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China.

通讯机构： [Huang, Shangzhi] S;[Chu, Pu] N;Sun YAT SEN Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China.;Sun YAT SEN Univ, Sch Life Sci, Guangdong Key Lab Plant Resource, Guangzhou 510275, Guangdong, Peoples R China.;Nanjing Agr Univ, Coll Agr, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China.

关键词： 1-cys peroxiredoxin;AtPER1;primary seed dormancy;ABA;ROS;Arabidopsis thaliana

摘要： Seed is vital to the conservation of germplasm and plant biodiversity. Seed dormancy is an adaptive trait in numerous seed-plant species, enabling plants to survive under stressful conditions. Seed dormancy is mainly controlled by abscisic acid (ABA) and gibberellin (GA) and can be classified as primary and secondary seed dormancy. The primary seed dormancy is induced by maternal ABA. Here we found that AtPER1, a seed-specific peroxiredoxin, is involved in enhancing primary seed dormancy. Two loss-of-function atper1 mutants, atper1-1 and atper1-2, displayed suppressed primary seed dormancy accompanied with reduced ABA and increased GA contents in seeds. Furthermore, atper1 mutant seeds were insensitive to abiotic stresses during seed germination. The expression of several ABA catabolism genes (CYP707A1, CYP707A2, and CYP707A3) and GA biosynthesis genes (GA20ox1, GA20ox3, and KAO3) in atper1 mutant seeds was increased compared to wild-type seeds. The suppressed primary seed dormancy of atper1-1 was completely reduced by deletion of CYP707A genes. Furthermore, loss-of-function of AtPER1 cannot enhance the seed germination ratio of aba2-1 or ga1-t, suggesting that AtPER1-enhanced primary seed dormancy is dependent on ABA and GA. Additionally, the level of reactive oxygen species (ROS) in atper1 mutant seeds was significantly higher than that in wild-type seeds. Taken together, our results demonstrate that AtPER1 eliminates ROS to suppress ABA catabolism and GA biosynthesis, and thus improves the primary seed dormancy and make the seeds less sensitive to adverse environmental conditions.

语种： 英文

作者： Su, Zhaohong;Xu, Xiaolin;Cheng, Yongbing;Tan, Yueming;Xiao, Langtao;...

期刊： Nanoscale,2019年11(3):962-967 ISSN：2040-3364

通讯作者： Qin, Xiaoli;Wang, Huixian

作者机构： [Qin, Xiaoli; Wang, Huixian; Su, Zhaohong; Xu, Xiaolin; Cheng, Yongbing; Tang, Daili; Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Tan, Yueming] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.;[Xiao, Langtao] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Qin, XL; Wang, HX] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.

关键词： Acetic acid;Antibodies;Chemical detection;Chemical modification;Cyclic voltammetry;Energy dispersive spectroscopy;Fourier transform infrared spectroscopy;Glass membrane electrodes;Gold nanoparticles;Graphene;High performance liquid chromatography;High resolution transmission electron microscopy;Immunosensors;Nanocomposite films;pH;Polycyclic aromatic hydrocarbons;Reduction;Scanning electron microscopy;Transmission electron microscopy;Ultraviolet visible spectroscopy;Bio-nanocomposite;Chemical impedance spectroscopy;Differential pulse voltammetry;Electrochemical immunosensors;Electrostatic adsorption;Energy dispersive spectroscopies (EDS);Glassy carbon electrodes;Indole-3-acetic acid;Film preparation;gold;graphite;immobilized antibody;indoleacetic acid;indoleacetic acid derivative;metal nanoparticle;nanocomposite;chemistry;electrochemical analysis;electrode;genetic procedures;immunology;limit of detection;oxidation reduction reaction;porosity;static electricity;Antibodies, Immobilized;Biosensing Techniques;Electrochemical Techniques;Electrodes;Gold;Graphite;Indoleacetic Acids;Limit of Detection;Metal Nanoparticles;Nanocomposites;Oxidation-Reduction;Porosity;Static Electricity

摘要： A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), elecro-chemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2 × 10 -11 to 2 × 10 -8 g mL -1 with a detection limit of 0.016 ng mL -1 (S/N = 3) and showed significant linearity R 2 = 0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranging from -5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite films for wide applications. © 2019 The Royal Society of Chemistry.

语种： 英文

作者： Li, Shuo;Wang, Nan;Ji, Dandan;Zhang, Wenxiao;Wang, Ying;...

期刊： PLANT CELL,2019年31(9):2107-2130 ISSN：1040-4651

通讯作者： Xiang, Fengning

作者机构： [Lyu, Menghua; Xiang, Fengning; Wang, Nan; Zhang, Wenxiao; Zhang, Yangyang; Li, Shuo; Ji, Dandan; Wang, Ying; Bai, Ming-yi; Wang, Xiaofang; Zhao, Shizhen; Yu, Yanchong; You, Juanjuan; Wang, Luli; Liu, Zhenhua] Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Shandong, Peoples R China.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Ji, Dandan] Qilu Univ Technol, Shandong Acad Sci, Coll Environm Sci & Engn, Jinan 250353, Shandong, Peoples R China.;[Yu, Yanchong] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Shandong, Peoples R China.

通讯机构： [Xiang, Fengning] S;Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Shandong, Peoples R China.

摘要： Abscisic acid (ABA) and reactive oxygen species (ROS) act as key signaling molecules in the plant response to salt stress; however, how these signals are transduced and amplified remains unclear. Here, a soybean (Glycine max) salinity-induced NAM/ATAF1/2/CUC2 (NAC) transcription factor encoded by SALT INDUCED NAC1 (GmSIN1) was shown to be a key component of this process. Overexpression of GmSIN1 in soybean promoted root growth and salt tolerance and increased yield under salt stress; RNA interference–mediated knockdown of GmSIN1 had the opposite effect. The rapid induction of GmSIN1 in response to salinity required ABA and ROS, and the effect of GmSIN1 on root elongation and salt tolerance was achieved by boosting cellular ABA and ROS contents. GmSIN1 upregulated 9-cis-epoxycarotenoid dioxygenase coding genes in soybean (GmNCED3s, associated with ABA synthesis) and Respiratory burst oxidase homolog B genes in soybean (GmRbohBs, associated with ROS generation) by binding to their promoters at a site that has not been described to date. Together, GmSIN1, GmNCED3s, and GmRbohBs constitute a positive feed-forward system that enables the rapid accumulation of ABA and ROS, effectively amplifying the initial salt stress signal. These findings suggest that the combined modulation of ABA and ROS contents enhances soybean salt tolerance. © 2019 American Society of Plant Biologists. All rights reserved.

语种： 英文

作者： Su, Zhaohong*;Cheng, Yongbing;Li, Chaorong;Xiong, Yuanfu;Xiao, Langtao;...

期刊： Nanoscale Advances,2019年1(9):3607-3613 ISSN：2516-0230

通讯作者： Su, Zhaohong;Qin, Xiaoli

作者机构： [Su, ZH; Qin, Xiaoli; Su, Zhaohong; Xiong, Yuanfu; Cheng, Yongbing; Li, Chaorong] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Xiao, Langtao] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.;[Chen, Shu] Hunan Univ Sci & Technol, Sch Chem & Chem Engn, Key Lab Theoret Organ Chem & Funct Mol, Minist Educ, Xiangtan 411201, Peoples R China.

通讯机构： [Su, ZH; Qin, XL] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.

关键词： Acetic acid;Antibodies;Biocompatibility;Cyclic voltammetry;Dispersions;Fiber optic sensors;Film preparation;Fourier transform infrared spectroscopy;Gold nanoparticles;High resolution transmission electron microscopy;Immunosensors;Metal nanoparticles;Nanocomposite films;Nanocomposites;Nanotubes;pH;Polycyclic aromatic hydrocarbons;Ultraviolet visible spectroscopy;2 ,5-dimercapto-1 ,3 ,4-thiadiazole;Amperometric immunosensors;Bio-nanocomposite materials;Differential pulse voltammetry;Fourier transform infrared spectra;Microwave-assisted methods;Multiwalled carbon nanotube (MWCNTs);Narrow size distributions;Multiwalled carbon nanotubes (MWCN)

摘要： An amperometric immunosensor based on new thiolated bionanocomposite with a high dispersion of gold nanoparticles (AuNPs) for the sensitive detection of indole-3-acetic acid (IAA) is being reported herein. Briefly, a thiolated nanocomposite was prepared via the microwave-assisted thiol-ene reaction of 2,5-dimercapto-1,3,4-thiadiazole (DMcT) with oxidized polyaniline (PANI), which was synthesized in the presence of multiwalled carbon nanotubes (MWCNTs), yielding thiolated polyaniline (TPANI)-MWCNTs. Further, AuNPs were deposited on the TPANI-MWCNTs by microwave-assisted method to obtain a AuNPs/TPANI-MWCNTs nanocomposite. Finally, the thiolated bionanocomposite film was constructed via the specific chemical reaction between boronic acid functionalized AuNPs and the vicinal diol functionalized AuNP labeled immunoglobulin G (IgG-AuNPs). The change in the reduction peak current of Fe(CN)63- was used to monitor the immunoreaction between IAA and antibody. The TPANI-MWCNT nanocomposites uniformly disperse AuNPs, IgG-AuNPs and anti-IAA-AuNPs, leading to the amplification of the signal of the immunosensor. Fourier transform infrared spectra (FTIR), cyclic voltammetry (CV), transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the nanocomposite film and the stepwise modification of the immunosensor. The prepared thiolated bionanocomposite material has good biocompatibility, a highly uniform dispersion of the AuNPs with a narrow size distribution as verified by TEM, and high load/activity of the immobilized antibody proved via DPV. The fabricated IAA amperometric immunosensor not only exhibits a good linear arrange from 1.0 pg mL-1 to 10 ng mL-1 with the limit of detection of 0.97 pg mL-1 (S/N = 3), but also possesses good selectivity, reproducibility and stability for the detection of IAA. © 2019 The Royal Society of Chemistry.

语种： 英文

作者： Ding, Pengcheng;Fang, Linlin;Wang, Guangling;Li, Xiang;Huang, Shu;...

期刊： Plant Molecular Biology,2019年101(1-2):203-220 ISSN：0167-4412

通讯作者： Chen, Fanguo

作者机构： [Fang, Linlin; Chen, Fanguo; Huang, Shu; Zhu, Jiantang; Gao, Yankun; Xia, Guangmin; Ding, Pengcheng; Wang, Guangling; Li, Xiang] Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Shandong, Peoples R China.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Hunan Prov Key Lab Phytohormones, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Chen, Fanguo] S;Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Shandong, Peoples R China.

关键词： Abscisic acid;Drought stress;Heme oxygenase 1;Methionine sulfoxide reductase A4.1;Reactive oxygen species;Salinity stress;Triticum aestivum

摘要： Key message: Here, a functional characterization of a wheat MSR has been presented: this protein makes a contribution to the plant’s tolerance of abiotic stress, acting through its catalytic capacity and its modulation of ROS and ABA pathways. Abstract: The molecular mechanism and function of certain members of the methionine sulfoxide reductase (MSR) gene family have been defined, however, these analyses have not included the wheat equivalents. The wheat MSR gene TaMSRA4.1 is inducible by salinity and drought stress and in this study, we demonstrate that its activity is restricted to the Met-S-SO enantiomer, and its subcellular localization is in the chloroplast. Furthermore, constitutive expression of TaMSRA4.1 enhanced the salinity and drought tolerance of wheat and Arabidopsis thaliana. In these plants constitutively expressing TaMSRA4.1, the accumulation of reactive oxygen species (ROS) was found to be influenced through the modulation of genes encoding proteins involved in ROS signaling, generation and scavenging, while the level of endogenous abscisic acid (ABA), and the sensitivity of stomatal guard cells to exogenous ABA, was increased. A yeast two-hybrid screen, bimolecular fluorescence complementation and co-immunoprecipitation assays demonstrated that heme oxygenase 1 (HO1) interacted with TaMSRA4.1, and that this interaction depended on a TaHO1 C-terminal domain. In plants subjected to salinity or drought stress, TaMSRA4.1 reversed the oxidation of TaHO1, activating ROS and ABA signaling pathways, but not in the absence of HO1. The aforementioned properties advocate TaMSRA4.1 as a candidate for plant genetic enhancement. © 2019, Springer Nature B.V.

语种： 英文

作者： Liu, Qing;Su, Yi;Zhu, Yunhua;Peng, Keqin;Hong, Bin;...

期刊： Biological Procedures Online,2019年21(1):1-14 ISSN：1480-9222

通讯作者： Xiao, Langtao

作者机构： [Su, Yi; Wang, Ruozhong; Liu, Qing; Peng, Keqin; Xiao, Langtao; Hong, Bin] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.;[Su, Yi; Wang, Ruozhong; Xiao, Langtao] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China.;[Zhu, Yunhua] Univ South China, Hengyang Med Coll, Hengyang 421001, Peoples R China.;[Gaballah, Mahmoud] Agr Res Ctr, Field Crops Res Inst, Rice Res & Training Ctr, Giza 33717, Egypt.

通讯机构： [Xiao, Langtao] H;Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha 410128, Hunan, Peoples R China.

关键词： Seasonal rice;Ratooning rice;Osa-MIR156f;Plant architecture;Grain yield

摘要： Rice (Oryza sativa L.) feeds more than half of the world’s population. Ratooning rice is an economical alternative to the second seasonal rice, thus increasing the yield of ratooning rice is highly important. Here we report an applicable transgenic line constructed through the manipulation of osa-MIR156f expression in rice shoot using the OsGA3ox2 (D18) promoter. In seasonal rice, the D18–11 transgenic line showed moderate height and more effective tillers with normal panicle. In ratooning rice, axillary buds outgrew from the basal node of the D18–11 transgenic line before the harvest of seasonal rice. More effective tillers produced by the outgrowth of axillary buds contributed to the plant architecture improvement and yield increase. Additionally, it was found that osa-miR156f down-regulated the expression of tillering regulators, such as TEOSINTE BRANCHED1 (TB1) and LAX PANICLE 1 (LAX1). The expression of DWARF10, DWARF27 and DWARF53, three genes being involved in the biosynthesis and signaling of strigolactone (SL), decreased in the stem of the D18–11 transgenic line. Our results indicated that the manipulation of osa-MIR156f expression may have application significance in rice genetic breeding. This study developed a novel strategy to regulate plant architecture and grain yield potential both in the seasonal and ratooning rice.

语种： 英文

作者： 刘锴栋;郑小林;肖浪涛;张涛;黎海利

作者机构： 岭南师范学院生命科学与技术学院;浙江工商大学食品与生物工程学院;湖南农业大学生物科学技术学院

会议名称： 第三届全国植物开花·衰老与采后生物学大会

会议时间： 2019-11-04

会议地点： 中国浙江杭州

关键词： 番木瓜;果实;成熟;生长素;乙烯;蛋白互作

摘要： 番木瓜（Carica papaya L.）属于呼吸跃变型果实,果实采后在常温下迅速转黄、软化并完成后熟,贮藏期较短,限制了番木瓜产业的发展。果实采后成熟是一个受到多种生理生化因素影响的复杂生物学过程,植物激素及植物激素信号途径在调控果实成熟中发挥重要的作用。本研究对番木瓜生长素响应因子基因CpARF2调控果实成熟的生理和分子机制进行了深入研究。克隆得到了CpARF2基因全长,基因表达分析表明果实采后成熟及外施乙烯诱导了CpARF2基因的表达,亚细胞定位分析显示CpARF2定位在细胞核中。利用番茄遗传转化体系证实异源超量表达CpARF2转基因番茄具有缩短果实从授粉到破色期天数、加快乙烯释放量高峰期的出现、并加速叶片衰老等作用。通过酵母双杂交、GST Pull-down、BiFC和LCI等技术,证明CpARF2不但能与自身形成二聚体,还能和1个重要乙烯信号途径蛋白实现体外体内互作。通过双荧光素酶报告系统,发现互作复合体能显著诱导CpACS1、CpACO1、CpXTH12和CpPE51等基因启动子的活性,从而调控番木瓜果实采后成熟的进程。本研究初步揭示CpARF2参与番木瓜果实采后成熟调控的分子机制。

语种： 中文

作者： Zhou, Chuanen;Han, Lu;Zhao, Yang;Wang, Hongfeng;Nakashima, Jin;...

期刊： PLANT JOURNAL,2019年100(3):562-571 ISSN：0960-7412

通讯作者： Wang, Zeng-Yu

作者机构： [Zhao, Yang; Zhou, Chuanen; Han, Lu; Wang, Hongfeng] Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Shandong, Peoples R China.;[Wang, Zeng-Yu; Nakashima, Jin] Noble Res Inst LLC, Ardmore, OK 73401 USA.;[Tong, Jianhua; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones, Changsha 410128, Hunan, Peoples R China.;[Wang, Zeng-Yu] Qingdao Agr Univ, Coll Grassland Sci, Grassland Agrihusb Res Ctr, Qingdao 266109, Shandong, Peoples R China.

通讯机构： [Wang, Zeng-Yu] N;[Wang, Zeng-Yu] Q;Noble Res Inst LLC, Ardmore, OK 73401 USA.;Qingdao Agr Univ, Coll Grassland Sci, Grassland Agrihusb Res Ctr, Qingdao 266109, Shandong, Peoples R China.

关键词： HD-ZIPIII gene family;REVOLUTA;leaf polarity;compound leaf patterning;Medicago

摘要： <jats:title>Summary</jats:title><jats:p>Leaves are derived from the shoot apical meristem with three distinct axes: dorsoventral, proximodistal and mediolateral. Different regulators are involved in the establishment of leaf polarity. Members of the class <jats:styled-content style="fixed-case">III</jats:styled-content> homeodomain‐leucine zipper (<jats:italic><jats:styled-content style="fixed-case">HD</jats:styled-content>‐<jats:styled-content style="fixed-case">ZIPIII</jats:styled-content></jats:italic>) gene family are critical players in the determination of leaf adaxial identity mediated by micro<jats:styled-content style="fixed-case">RNA</jats:styled-content>165/166. However, their roles in compound leaf development are still unclear. By screening of a retrotransposon‐tagged mutant population of the model legume plant <jats:italic>Medicago truncatula</jats:italic>, a mutant line with altered leaflet numbers was isolated and characterized. Mutant leaves partially lost their adaxial identity. Leaflet numbers in the mutant were increased along the proximodistal axis, showing pinnate pentafoliate leaves in most cases, in contrast to the trifoliate leaves of the wild type. Detailed characterization revealed that a lesion in a <jats:italic><jats:styled-content style="fixed-case">HD</jats:styled-content>‐<jats:styled-content style="fixed-case">ZIPIII</jats:styled-content></jats:italic> gene, <jats:styled-content style="fixed-case">REVOLUTA</jats:styled-content> (<jats:italic>Mt<jats:styled-content style="fixed-case">REV</jats:styled-content>1</jats:italic>), resulted in the defects of the mutant. Overexpression of Mt<jats:styled-content style="fixed-case">MIR</jats:styled-content>166‐insensitive <jats:italic>Mt<jats:styled-content style="fixed-case">REV</jats:styled-content>1</jats:italic> led to adaxialized leaves and ectopic leaflets along the dorsoventral axis. Accompanying the abnormal leaf patterning, the free auxin content was affected. Our results demonstrate that <jats:italic>Mt<jats:styled-content style="fixed-case">REV</jats:styled-content>1</jats:italic> plays a key role in determination of leaf adaxial–abaxial polarity and compound leaf patterning, which is associated with proper auxin homeostasis.</jats:p>

语种： 英文

作者： Guo, Tingting;Wang, Daofeng;Fang, Jingjing;Zhao, Jinfeng;Yuan, Shoujiang;...

期刊： International Journal of Molecular Sciences,2019年20(10):2567 ISSN：1661-6596

通讯作者： Xiao, Langtao;Li, Xueyong

作者机构： [Guo, Tingting; Xiao, Langtao] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Key Lab Phytohormones, Changsha 410128, Hunan, Peoples R China.;[Fang, Jingjing; Li, Xueyong; Wang, Daofeng; Zhao, Jinfeng] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China.;[Yuan, Shoujiang] Shandong Rice Res Inst, Jinan 250100, Shandong, Peoples R China.

通讯机构： [Xiao, Langtao] H;[Li, Xueyong] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Hunan Prov Key Lab Phytohormones, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China.

关键词： acetic acid;auxin;helicase;nuclear protein;pickle related1 protein;PKR1 protein;protein CHR702;protein CHR703;protein CHR705;protein CHR723;protein CHR729;protein CHR744;unclassified drug;DNA helicase;plant protein;wax;agronomic trait;Article;chromatin assembly and disassembly;chromatin immunoprecipitation;controlled study;drought tolerance;epigenetic repression;evapotranspiration;frameshift mutation;gene expression;gene mutation;histone modification;Indian rice;leaf lamina;leaf rolling;mass fragmentography;morphogenesis;nonhuman;OsCHR4 gene;phenotype;plant gene;plant genetics;plant height;plant stress;real time polymerase chain reaction;reverse transcription polymerase chain reaction;scanning electron microscopy;upregulation;water loss;chromatin assembly and disassembly;drought;gene expression regulation;genetic epigenesis;genetics;metabolism;Oryza;physiological stress;physiology;plant leaf;transgenic plant;ultrastructure;Chromatin Assembly and Disassembly;DNA Helicases;Droughts;Epigenesis, Genetic;Frameshift Mutation;Gene Expression Regulation, Plant;Oryza;Phenotype;Plant Leaves;Plant Proteins;Plants, Genetically Modified;Stress, Physiological;Waxes

摘要： Leaf blade width, curvature, and cuticular wax are important agronomic traits of rice. Here, we report the rice Oschr4-5 mutant characterized by pleiotropic phenotypes, including narrow and rolled leaves, enhanced cuticular wax deposition and reduced plant height and tiller number. The reduced leaf width is caused by a reduced number of longitudinal veins and increased auxin content. The cuticular wax content was significantly higher in the Oschr4-5 mutant, resulting in reduced water loss rate and enhanced drought tolerance. Molecular characterization reveals that a single-base deletion results in a frame-shift mutation from the second chromodomain of OsCHR4, a CHD3 (chromodomain helicase DNA-binding) family chromatin remodeler, in the Oschr4-5 mutant. Expressions of seven wax biosynthesis genes (GL1-4, WSL4, OsCER7, LACS2, LACS7, ROC4 and BDG) and four auxin biosynthesis genes (YUC2, YUC3, YUC5 and YUC6) was up-regulated in the Oschr4-5 mutant. Chromatin immunoprecipitation assays revealed that the transcriptionally active histone modification H3K4me3 was increased, whereas the repressive H3K27me3 was reduced in the upregulated genes in the Oschr4-5 mutant. Therefore, OsCHR4 regulates leaf morphogenesis and cuticle wax formation by epigenetic modulation of auxin and wax biosynthetic genes expression. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Luo, Weigui;Xiao, Yuan;Liang, Qiwen;Su, Yi*;Xiao, Langtao*

期刊： Molecules,2019年24(17):3146 ISSN：1420-3049

通讯作者： Su, Yi;Xiao, Langtao

作者机构： [Su, Y; Xiao, Langtao; Su, Yi; Liang, Qiwen; Luo, Weigui] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.;[Xiao, Yuan] Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China.

通讯机构： [Su, Y; Xiao, LT] H;Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.

关键词： Arabidopsis protein;indoleacetic acid derivative;oligopeptide;phytohormone;Arabidopsis;biosynthesis;classification;cytology;drug effect;gene expression;gene expression profiling;genetics;isolation and purification;metabolism;plant cell;plant leaf;procedures;proteomics;protoplast;signal transduction;transgenic plant;Arabidopsis;Arabidopsis Proteins;Gene Expression;Gene Expression Profiling;Indoleacetic Acids;Oligopeptides;Plant Cells;Plant Growth Regulators;Plant Leaves;Plants, Genetically Modified;Proteomics;Protoplasts;Signal Transduction

摘要： Small signaling peptides (SSPs) are a class of short peptides playing critical roles in plant growth and development. SSPs are also involved in the phytohormone signaling pathway. However, identification of mature SSPs is still a technical challenge because of their extremely low concentrations in plant tissue and complicated interference by many other metabolites. Here, we report an optimized protocol to extract SSPs based on protoplast extraction and to analyze SSPs based on tandem mass spectrometry peptidomics. Using plant protoplasts as the material, soluble peptides were directly extracted into phosphate buffer. The interference of non-signaling peptides was significantly decreased. Moreover, we applied the protocol to identify potential SSPs in auxin treated wild type and auxin biosynthesis defective mutant yuc2yuc6. Over 100 potential SSPs showed a response to auxin in Arabidopsis thaliana. © 2019 by the authors.

语种： 英文

作者： Su, Yi;Li, Wei;Huang, Zhigang;Wang, Ruozhong;Luo, Weigui;...

期刊： Plant Methods,2018年14(1):1-10 ISSN：1746-4811

通讯作者： Xiao, Langtao

作者机构： [Huang, Zhigang; Su, Yi; Wang, Ruozhong; Tong, Jianhua; Li, Wei; Luo, Weigui; Liu, Qing; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China.;[Huang, Zhigang; Su, Yi; Wang, Ruozhong; Liu, Qing; Xiao, Langtao] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.;[Li, Wei] Hunan Acad Agr Sci, Tea Res Inst, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Xiao, Langtao] H;Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China.

关键词： qIPCR;ABA;Biotin;Avidin

摘要： Abscisic acid (ABA) functions as a stress phytohormone in many growth and developmental processes in plants. The ultra-sensitive determination of ABA would help to better understand its vital roles and action mechanisms. We report a new sensitive and high throughput quantitative real time immuno-PCR (qIPCR) method based on biotin–avidin linkage system for ABA determination in plants. ABA monoclonal antibody (McAb) coated on the inner surface of PCR well pretreated with glutaraldehyde. The pre-prepared probe complex, including biotinylated McAb, biotinylated DNA and streptavidin linker, was convenient for high throughput operations. Finally, probe DNA was quantified by real-time PCR. The detectable ranges were from 10 to 40 ng/L with a limit of detection (LOD) of 2.5 fg. ABA contents in plant sample were simultaneously analyzed using LC–MS/MS to validate the qIPCR method. The results showed that qIPCR method has good specificity and repeatability with a recovery rate of 96.9%. The qIPCR method is highly sensitive for ABA quantification for actual plant samples with an advantage of using crude extracts instead of intensively purified samples.

语种： 英文

作者： Zhang, Ke;Wang, Ruozhong;Zi, Hailing;Li, Yongpeng;Cao, Xiuwei;...

期刊： PLANT CELL,2018年30(2):324-346 ISSN：1040-4651

通讯作者： Liu, Xigang

作者机构： [Zhang, Ke; Li, Dongming; Li, Yongpeng; Guo, Lin; Liu, Xigang] Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, State Key Lab Plant Cell & Chromosome Engn, Shijiazhuang 050024, Hebei, Peoples R China.;[Zhang, Ke; Li, Dongming; Li, Yongpeng; Guo, Lin; Liu, Xigang] Hebei Collaborat Innovat Ctr Cell Signaling, Shijiazhuang 050024, Hebei, Peoples R China.;[Zhang, Ke; Cao, Xiuwei; Zi, Hailing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Tong, Jianhua; Xiao, Langtao; Wang, Ruozhong] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.;[Zi, Hailing; Liu, Renyi] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China.

通讯机构： [Liu, Xigang] C;[Liu, Xigang] H;Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, State Key Lab Plant Cell & Chromosome Engn, Shijiazhuang 050024, Hebei, Peoples R China.;Hebei Collaborat Innovat Ctr Cell Signaling, Shijiazhuang 050024, Hebei, Peoples R China.

摘要： Successful floral meristem (FM) determinacy is critical for subsequent reproductive development and the plant life cycle. Although the phytohormones cytokinin and auxin interact to coregulate many aspects of plant development, whether and how cytokinin and auxin function in FM determinacy remain unclear. Here, we show that in Arabidopsis thaliana, cytokinin homeostasis is critical for FM determinacy. In this developmental context, auxin promotes the expression of AUXIN RESPONSE FACTOR3 (ARF3) to repress cytokinin activity. ARF3 directly represses the expression of ISOPENTENYLTRANSFERASE (IPT) family genes and indirectly represses LONELY GUY (LOG) family genes, both of which encode enzymes required for cytokinin biosynthesis. ARF3 also directly inhibits the expression of ARABIDOPSIS HISTIDINE KINASE4, a cytokinin receptor gene, resulting in reduced cytokinin activity. Consequently, ARF3 controls cell division by regulating cell cycle gene expression through cytokinin. In flowers, we show that AGAMOUS (AG) dynamically regulates the expression of ARF3 and IPTs, resulting in coordinated regulation of FM maintenance and termination through cell division. Moreover, genome-wide transcriptional profiling revealed both repressive and active roles for ARF3 in early flower development. Our findings establish a molecular link between AG and auxin/cytokinin and shed light on the mechanisms of stem cell maintenance and termination in the FM.

语种： 英文

作者： 郭滢;黄兴传;杨程惠子;刘忠松;彭克勤;...

作者机构： [郭滢; 黄兴传; 杨程惠子; 刘忠松; 彭克勤; 肖浪涛; 夏石头] 湖南农业大学植物激素与生长发育湖南省重点实验室南方粮油作物协同创新中心;[郭滢; 黄兴传; 杨程惠子; 刘忠松; 彭克勤; 肖浪涛; 夏石头] 湖南农业大学生物科学技术学院;[郭滢; 黄兴传; 杨程惠子; 刘忠松; 彭克勤; 肖浪涛; 夏石头] 湖南农业大学农学院南方粮油作物协同创新中心

会议名称： 中国植物学会第十六次全国会员代表大会暨85周年学术年会

会议时间： 2018-10-10

会议地点： 中国云南昆明

关键词： 黄籽油菜;种籽颜色;含油量;不饱和脂肪酸;植物激素

摘要： 我国是芥菜型油菜起源地之一,资源丰富。种子颜色既是植物驯化现状,又是重要的遗传标记性状与作物品质的指示性状。本研究以芥菜型油菜黄色品种"四川黄籽"及其近等基因系黑籽油菜和甘蓝型油菜黑色品种及芥甘杂交后代黄色和黑色品种种质为材料,通过植株生长发育与农艺性状观测、不同阶段种子发育的解剖结构与特性、植物激素含量和种籽发育基因表达分析等,以揭示黄籽油菜的生物学特性。研究发现油菜种籽发育过程中,黄籽油菜和黑

语种： 中文