Integrated Metabolome and Transcriptome Analysis Unveils Novel Pathway Involved in the Formation of Yellow Peel in Cucumber
作者：
Chen, Chen;Zhou, Geng;Chen, Juan;Liu, Xiaohong;Lu, Xiangyang;...
期刊：
International Journal of Molecular Sciences ,2021年22(3):1-19 ISSN：1661-6596
通讯作者：
Chen, Huiming;Tian, Yun
作者机构：
[Tian, Yun; Chen, Chen; Chen, Huiming; Lu, Xiangyang; Chen, HM; Tian, Y] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Chen, Chen; Chen, Huiming; Liu, Xiaohong; Zhou, Geng; Chen, Juan] Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China.;[Chen, Huiming; Zhou, Geng] Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China.
通讯机构：
[Chen, HM; Tian, Y; Chen, Huiming] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China.;Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China.
关键词：
flavonoid;mitogen activated protein kinase;transcriptome;carotenoid;flavone derivative;flavonoid;homoeriodictyol;messenger RNA;pigment;plant DNA;plant protein;plant RNA;transcription factor;transcriptome;Article;biosynthesis;cucumber;fruit color;fruit peel;gene expression;MAPK signaling;metabolome;nonhuman;phenotype;real time polymerase chain reaction;varietas;cucumber;fruit;gene expression regulation;gene ontology;genetics;metabolism;pigmentation;plant breeding;plant epidermis;plant gene;Carotenoids;Cucumis sativus;DNA, Plant;Flavones;Flavonoids;Fruit;Gene Expression Regulation, Plant;Gene Ontology;Genes, Plant;MAP Kinase Signaling System;Metabolome;Pigmentation;Pigments, Biological;Plant Breeding;Plant Epidermis;Plant Proteins;RNA, Messenger;RNA, Plant;Transcription Factors;Transcriptome
摘要：
Yellow peel will adversely affect the appearance quality of cucumber fruit, but the metabolites and the molecular mechanism of pigment accumulation in cucumber peel remain unclear. Flavonoid metabolome and transcriptome analyses were carried out on the young peel and old peel of the color mutant L19 and the near-isogenic line L14. The results showed that there were 165 differential flavonoid metabolites in the old peel between L14 and L19. The total content of representative flavonoid metabolites in the old peel of L14 was 95 times that of L19, and 35 times that of young peel of L14, respectively. This might explain the difference of pigment accumulation in yellow peel. Furthermore, transcriptome analysis showed that there were 3396 and 1115 differentially expressed genes in the yellow color difference group (Young L14 vs. Old L14 and Old L14 vs. Old L19), re-spectively. These differentially expressed genes were significantly enriched in the MAPK signaling pathway–plant, plant–pathogen interaction, flavonoid biosynthesis and cutin, suberine and wax bi-osynthesis pathways. By analyzing the correlation between differential metabolites and differen-tially expressed genes, six candidate genes related to the synthesis of glycitein, kaempferol and ho-moeriodictyol are potentially important. In addition, four key transcription factors that belong to R2R3-MYB, bHLH51 and WRKY23 might be the major drivers of transcriptional changes in the peel between L14 and L19. Then, the expression patterns of these important genes were confirmed by qRT-PCR. These results suggested that the biosynthesis pathway of homoeriodictyol was a novel way to affect the yellowing of cucumber peel. Together, the results of this study provide a research basis for the biosynthesis and regulation of flavonoids in cucumber peel and form a significant step towards identifying the molecular mechanism of cucumber peel yellowing. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Yarrowia lipolytica as an Oleaginous Platform for the Production of Value-Added Fatty Acid-Based Bioproducts
作者：
Liu, Huhu* ;Song, Yulan;Fan, Xiao;Wang, Chong;Lu, Xiangyang;...
期刊：
Frontiers in Microbiology ,2021年11:608662 ISSN：1664-302X
通讯作者：
Liu, Huhu;Tian, Yun
作者机构：
[Fan, Xiao; Tian, Yun; Liu, Huhu; Wang, Chong; Lu, Xiangyang; Song, Yulan; Liu, HH; Tian, Y] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
通讯机构：
[Liu, HH; Tian, Y] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
关键词：
yarrowia lipolytica;cell factory;fatty acid metabolism;bioproducts;Metabolic Engineering
摘要：
The microbial fermentation process has been used as an alternative pathway to the production of value-added natural products. Of the microorganisms, Yarrowia lipolytica, as an oleaginous platform, is able to produce fatty acid-derived biofuels and biochemicals. Nowadays, there are growing progresses on the production of value-added fatty acid-based bioproducts in Y. lipolytica. However, there are fewer reviews performing the metabolic engineering strategies and summarizing the current production of fatty acid-based bioproducts in Y. lipolytica. To this end, we briefly provide the fatty acid metabolism, including fatty acid biosynthesis, transportation, and degradation. Then, we introduce the various metabolic engineering strategies for increasing bioproduct accumulation in Y. lipolytica. Further, the advanced progress in the production of fatty acid-based bioproducts by Y. lipolytica, including nutraceuticals, biofuels, and biochemicals, is summarized. This review will provide attractive thoughts for researchers working in the field of Y. lipolytica.
语种：
英文
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拉萨地区青稞种植土壤中细菌多样性高通量分析
作者：
刘青海;潘虎;达娃卓玛;田云;刘虎虎;...
期刊：
生物学杂志 ,2020年37(2):46-51 ISSN：2095-1736
作者机构：
[刘青海; 达娃卓玛; 白军平] 西藏自治区农牧科学院农业质量标准与检测研究所;湖南农业大学生物科学技术学院;[潘虎; 王翀; 田云; 卢向阳; 刘虎虎] 湖南农业大学
关键词：
拉萨;青稞;农田土壤;细菌多样性;高通量测序
摘要：
为了解拉萨地区青稞种植农田土壤中细菌群落结构,明确不同青稞生长时期和不同地点对细菌多样性的影响,以青稞种植土壤为试验材料,通过高通量测序技术分析青稞种植土壤中细菌的群落结构及多样性。结果显示:本次种植青稞土壤微生物测序每个样品获得37 265 ~ 40 008条有效序列和4094 ~ 4730个OTU,稀释曲线和测序深度指数反映此次测序结果比较全面地覆盖了土壤微生物群落;不同时期各样品的Shannon指数和OTU数量均值大小为Group 1(3月25日、播种前期)< Group 2(7月8日、灌浆期)< Group 3(9月28日、收获后期),不同时期菌落组成和菌落数量在Gp16、Gaiella、Ilumatobacter和Nocardioides等存在差异;随着青稞的生长发育,其土壤样品中细菌多样性和细菌数量逐渐增加;青稞播种前期和收获后期不同地点土壤细菌丰富度指数高于灌浆期;土壤细菌群落优势种群主要为酸杆菌门(Acidobacteria)的Gp6、Gp4、Gp7和Gp16,芽单胞菌门(Gemmatimonas)的鞘氨醇单胞菌属(Sphingomonas)和芽生球菌属(Blastococcus),放线菌门的Gaiella、节细菌属(Arthrobacter)和类诺卡氏菌属(Nocardioides)等。青稞播种前期和收获后期不同地区土壤细菌群落组成差异显著,青稞播种前期6号和9号样品细菌群落同时含有的Ohtaekwangia是同时期其他样品中所没有的,青稞收获后期3号和4号样品细菌群落分别含有的Opitutus和假诺卡氏菌属(Pseudonocardia)与其他样品微生物群落组成差异显著。
语种：
中文
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Assessment of genetic diversity in Camellia oleifera Abel. accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers
作者：
Xiao, Pu;Liu, Huhu;Wang, Dongping;Tang, Wenyan;Yang, Hui;...
期刊：
Genetic Resources and Crop Evolution ,2020年67(5):1115-1124 ISSN：0925-9864
通讯作者：
Tian, Yun;Chen, Yongzhong
作者机构：
[He, Zhilong; Tian, Yun; Xiao, Pu; Liu, Huhu; Wang, Chong; Lu, Xiangyang; Tang, Wenyan; Wang, Dongping; Yang, Hui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Tian, Yun; Xiao, Pu; Liu, Huhu; Wang, Chong; Lu, Xiangyang; Tang, Wenyan; Wang, Dongping; Yang, Hui] Hunan Prov Univ, Key Lab Agr Biochem & Biotransformat, Changsha 410128, Hunan, Peoples R China.;[He, Zhilong; Chen, Yongzhong; Wang, Xiangnan; Wang, Rui] Natl Engn Res Ctr Oil Tea Camellia, Changsha 410004, Hunan, Peoples R China.;[He, Zhilong; Chen, Yongzhong; Wang, Xiangnan; Wang, Rui] Hunan Acad Forestry, Changsha 410004, Hunan, Peoples R China.;[Tian, Yun; Lu, Xiangyang] Hunan Opt Agr Engn Technol Res Ctr, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Tian, Yun] H;[Chen, Yongzhong] N;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Natl Engn Res Ctr Oil Tea Camellia, Changsha 410004, Hunan, Peoples R China.
关键词：
Genetic diversity;ISSR;SCoT;Camellia oleifera
摘要：
Camellia oleifera Abel. is a cultivable plant with important economic value. It is very helpful for the scientific utilization, cultivation and preservation of germplasm resources through evaluating the genetic diversity. In this study, we estimated the genetic distances of 135 accessions of C. oleifera using ISSR and SCoT markers, respectively. The genetic dissimilarity coefficients of ISSR markers ranged from 0.56 to 1.00. And the accessions were divided into five groups. While the genetic dissimilarity coefficients of SCoT markers ranged from 0.62 to 1.00, the accessions were divided into four groups. The results demonstrated that C. oleifera germplasms perform a little genetic variation. This is the first report to evaluate the genetic diversity of different C. oleifera germplasms using the ISSR and SCoT molecular markers, and the results provide the useful foundation for plant breeding of C. oleifera.
语种：
英文
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黄瓜果皮颜色的遗传规律与分子机理
作者：
陈宸;周赓;刘晓虹;卢向阳;陈惠明;...
期刊：
分子植物育种 ,2020年18(12):4029-4035 ISSN：1672-416X
作者机构：
湖南农业大学生物科学技术学院, 长沙, 410128;湖南大学研究生院隆平分院, 长沙, 410125;湖南省农业科学院湖南省蔬菜研究所, 长沙, 410125;[田云; 卢向阳; 陈宸] 湖南农业大学;[陈惠明; 刘晓虹] 湖南省蔬菜研究所
关键词：
黄瓜(Cucumis sativus L.);果皮颜色;分子标记;遗传调控
摘要：
黄瓜(Cucumis sativus L.)是重要蔬菜作物之一。黄瓜果皮颜色作为最直接的外观品质,具有重要的商品价值。黄瓜果皮颜色性状为数量性状,其遗传表达受到环境的影响。目前控制黄瓜果皮颜色的5个主要基因已被克隆。其中CsaARC5和CsYcf54控制黄瓜浅绿色果皮,w基因控制白色果皮,CsMYB36基因控制黄绿色果皮以及CsMYB60基因控制橙色果皮。它们均通过调控叶绿素以及类黄酮代谢途径来决定黄瓜果皮颜色。本综述总结了近年来黄瓜果皮颜色的遗传规律与分子机理的研究进展,为进一步完善黄瓜果皮颜色调控机制提供参考。
语种：
中文
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Porous lamellar carbon assembled from Bacillus mycoides as high-performance electrode materials for vanadium redox flow batteries
作者：
Deng, Qi;Tian, Yun;Ding, Ping;Yue, Junpei;Zeng, Xian-Xiang;...
期刊：
Journal of Power Sources ,2020年450:227633 ISSN：0378-7753
通讯作者：
Wu, Xiong-Wei;Lu, Xiang-Yang;Guo, Yu-Guo
作者机构：
[Tian, Yun; Wu, Xiong-Wei; Deng, Qi; Zeng, Xian-Xiang; Lu, Xiang-Yang; Wu, XW] Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Sci, Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;[Ding, Ping] Cent South Univ, XiangYa Sch Publ Hlth, Changsha 410078, Hunan, Peoples R China.;[Yue, Junpei; Yin, Ya-Xia; Guo, Yu-Guo] Chinese Acad Sci, Univ Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci,BNLMS,Inst Ch, CAS Key Lab Mol Nanostruct & Nanotechnol,CAS Res, Beijing 100190, Peoples R China.;[Yin, Ya-Xia; Guo, Yu-Guo] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
通讯机构：
[Wu, XW; Lu, XY] H;[Guo, Yu-Guo] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Coll Sci, Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Univ Chinese Acad Sci, CAS Res Educ Ctr Excellence Mol Sci,BNLMS,Inst Ch, CAS Key Lab Mol Nanostruct & Nanotechnol,CAS Res, Beijing 100190, Peoples R China.
关键词：
Bacteriology;Carbon;Catalyst activity;Charging (batteries);Electrodes;Energy efficiency;Redox reactions;Bacillus mycoides;Electrocatalytic activity;Electrochemical systems;Electronic conductivity;High current densities;High energy efficiency;Rapid charge migration;Vanadium redox flow batteries;Flow batteries
摘要：
The demand for clean and renewable energy has promoted the rapid development of state-of-the-art energy storage electrochemical systems. Vanadium redox flow batteries attract much more attentions due to low cost and high security, unfortunately the low catalytic activity of electrodes for vanadium ion redox process is the main barrier to achieve a high efficiency. This work proposes a novel composite electrode derived from Bacillus mycoides to overcome the dilemma between high degree of graphitization and uniform heteroatom doping. Consequently, this composite electrode simultaneously achieves rapid charge migration and high electrocatalytic activity and enhances the vanadium ion redox reaction on porous lamellar carbon (PLC) materials. Compared with the pristine electrode, the potential polarization for V(IV)/V(V) and V(II)/V(III) redox couples on PLC electrode decrease by 254 mV and 278 mV, respectively. Moreover, a high stability for cycling continuously for over 1000 cycles at a high current density of 200 mA cm−2 and a high energy efficiency can be realized based on PLC electrode. This work not only paves the way to achieve high energy efficiency of vanadium redox flow batteries, but also provides a feasible and novel way to design carbon materials with high catalytic activity and high electronic conductivity. © 2019 Elsevier B.V.
语种：
英文
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退化蛋白/上皮钠通道超家族成员的激活与调控机制
作者：
唐泽华;杨晓娜;卢向阳;牛友芽;田云
期刊：
生命科学 ,2020年32(7):723-730 ISSN：1004-0374
作者机构：
[牛友芽] 湖南农业大学生物科学技术学院,长沙410128;[牛友芽] 湖南医药学院医学院,怀化418000;[杨晓娜; 田云; 唐泽华; 卢向阳] 湖南农业大学
关键词：
退化蛋白/上皮钠通道超家族;离子通道;激活机制;小分子配体
摘要：
退化蛋白/上皮钠通道(DEG/ENaC)超家族是20世纪90年代发现的一类非电压依赖性的、阿米诺利敏感的钠离子选择性离子通道,其激活方式复杂多样,参与如囊肿性纤维化、焦虑及癫痫等多种生理病理过程,已成为多种疾病的潜在药物靶标。现就DEG/ENaC超家族成员的结构、特异激活方式以及交互激活与调控机制进行综述。
语种：
中文
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Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt
作者：
Zhang, Yi;Tian, Cheng;Xiao, Jiling;Wei, Lin;Tian, Yun* ;...
期刊：
AMB EXPRESS ,2020年10(1):1-13 ISSN：2191-0855
通讯作者：
Tian, Yun;Liang, Zhihuai
作者机构：
[Tian, Yun; Zhang, Yi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Xiao, Jiling; Tian, Cheng; Zhang, Yi; Liang, Zhihuai] Hunan Acad Agr Sci, Inst Agr Biotechnol Res, Changsha, Hunan, Peoples R China.;[Wei, Lin] Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Hunan, Peoples R China.
通讯机构：
[Tian, Yun; Liang, Zhihuai] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Hunan Acad Agr Sci, Inst Agr Biotechnol Res, Changsha, Hunan, Peoples R China.
关键词：
Trichoderma asperellum M45a;Fusarium wilt;Rhizosphere microorganism;Watermelon
摘要：
<jats:title>Abstract</jats:title><jats:p>Fusarium wilt (FW) caused by <jats:italic>Fusarium oxysporum</jats:italic> f. sp. <jats:italic>niveum</jats:italic> (FON) is a soil-borne disease that seriously limits watermelon production. In the present study, <jats:italic>Trichoderma asperellum</jats:italic> (<jats:italic>T. asperellum</jats:italic>) M45a was shown to be an effective biocontrol agent against FW. In a pot experiment, the application of 10<jats:sup>5</jats:sup>cfu/g of <jats:italic>T. asperellum</jats:italic> M45a granules had an improved control effect on FW during the blooming period (up to 67.44%) in soils subjected to five years of continuous cropping with watermelon, while the average length of watermelon vines was also significantly improved (P < 0.05). Additionally, the acid phosphatase (ACP), cellulase (CL), catalase (CAT), and sucrase (SC) activities in the M45a-inoculation group were significantly higher than those in the control (CK) group, and transformation of the soil nutrients (total N, NO3-N, and available P) was significantly increased. Moreover, <jats:italic>T. asperellum</jats:italic> M45a inoculation reduced fungal diversity, increased bacterial diversity and especiallyenhanced the relative abundance of plant growth-promoting rhizobacteria (PGPR), such as <jats:italic>Trichoderma, Sphingomonas</jats:italic>, <jats:italic>Pseudomonas</jats:italic>, <jats:italic>Actinomadura</jats:italic>, and <jats:italic>Rhodanobacter</jats:italic>. Through functional prediction, the relative abundance of ectomycorrhiza, endophytes, animal pathotrophs, and saprotrophs in the fungal community was determined to be significantly lower than that observed in the M45a-treated soil. Correlation analysis revealed that <jats:italic>Sphingomonas</jats:italic>, <jats:italic>Pseudomonas</jats:italic>, and <jats:italic>Trichoderma</jats:italic> had the most differences in terms of microorganism abundance, and these differences were positively correlated with ACP, CL, CAT, and SC. These findings provide guidance for the use of fungicides to achieve microecological control of FW in continuously cropped watermelon plots.</jats:p>
语种：
英文
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鱼源致病性类志贺邻单胞菌的研究进展
作者：
陈美群;王冬平;潘瑛子;张驰;扎西拉姆;...
期刊：
水产科学 ,2020年39(5):780-786 ISSN：1003-1111
作者机构：
西藏自治区农牧科学院水产科学研究所, 西藏, 拉萨, 850032;湖南农业大学生物科学技术学院, 湖南, 长沙, 410128;[田云; 王冬平] 湖南农业大学;[潘瑛子; 陈美群; 张驰; 扎西拉姆] 西藏自治区农牧科学院
关键词：
类志贺邻单胞菌;生物学特性;致病性;抗原特性;药敏特性
摘要：
类志贺邻单胞菌(Plesiomonas shigelloides)为肠杆菌科、邻单胞菌属内唯一种[1],广泛分布于自然界,是一种全球性分布细菌,水生态环境是其主要储存地,而鱼类是其常见宿主,可通过受污染的水、鱼及贝类感染人类[2-6]。类志贺邻单胞菌是一种人—兽—鱼共患病原菌[7],可导致鱼类等水产动物疾病频发,同时也可引起人类的肠胃炎、败血症、脑膜炎、腹泻和食物中毒等疾病[8-10],因此受到国内外学者的广泛关注。
语种：
中文
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菌肥对藏青2000生长、品质及根际土壤性质的影响
作者：
潘虎;刘青海;田云;达娃卓玛;张唐伟;...
期刊：
生物学杂志 ,2020年37(1):67-71 ISSN：2095-1736
作者机构：
[潘虎] 西藏自治区农牧科学院 农业质量标准与检测研究所,拉萨850032;[潘虎] 湖南农业大学 生物科学技术学院,长沙410128;[田云; 卢向阳] 湖南农业大学;[张唐伟; 刘青海; 达娃卓玛; 白军平] 西藏自治区农牧科学院农业质量标准与检测研究所
关键词：
微生物肥料;藏青2000;土壤理化性质
摘要：
为明确微生物菌肥对藏青2000生长、品质及根际土壤性质的影响,对5组不同处理藏青2000的8个生长指标、9个品质指标和8个土壤理化指标进行了检测分析。结果表明无机化肥+微生物施肥组(LSW4)处理的藏青2000生长发育较为良好,产量能够达到2467. 52 kg/hm~2,较无机化肥组(LSW2)产量提高39. 76%; 5组不同施肥处理的藏青2000粗蛋白质、粗淀粉、粗纤维含量差异较小,但LSW4处理的藏青2000钙(Ca)、铁(Fe)、镁(Mg)、锰(Mn)、锌(Zn)和铜(Cu)等矿质元素较其他处理组均有不同程度提高; LSW4处理较其他处理组能够提高植物对土壤氮、磷的吸收利用,但对钾的吸收利用差异较小,微生物与无机化肥配合施用对提高藏青2000实际产量增效显著。
语种：
中文
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气相色谱法测定茄果类蔬菜中12种有机磷类农药残留
作者：
蒲继锋;潘虎;代艳娜;张一帆;次顿;...
期刊：
食品安全质量检测学报 ,2020年11(4):1192-1196 ISSN：2095-0381
作者机构：
[潘虎] 西藏自治区农牧科学院农业质量标准与检测研究所,拉萨 850032;[潘虎] 湖南农业大学生物科学技术学院,长沙 410128;四川省农业科学院分析测试中心,成都 610066;[代艳娜; 张一帆; 蒲继锋; 次顿] 西藏自治区农牧科学院农业质量标准与检测研究所;[田云] 湖南农业大学
关键词：
农药残留;气相色谱法;茄果类蔬菜
摘要：
目的 建立气相色谱法同时测定茄果类蔬菜中12种有机磷类农药的残留分析方法.方法 样品用乙腈提取、旋蒸后经有机微孔滤膜净化,采用DB-1701毛细管色谱柱程序升温分离,采用火焰光度检测器(flame photometric detector,FPD)测定.结果 在本试验条件下,12种有机磷类农药分离效果较好,在0.05～1.0 mg/L范围内呈现良好的线性关系,相关系数r值为0.99935～0.99993,回收率为71.5％～112.4％,RSD值为1.0％～9.4％,检出限为0.01～0.06 mg/kg.结论 该方法快速、准确,适合茄果类蔬菜中多种农药残留定量检测要求.
语种：
中文
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葛仙米化学成分及生理活性研究进展
作者：
易思富;田云
期刊：
食品研究与开发 ,2020年41(9):189-194 ISSN：1005-6521
作者机构：
湖南农业大学生物科学技术学院,湖南长沙410128;常德炎帝生物科技有限公司,湖南常德415707;[田云; 易思富] 湖南农业大学
关键词：
葛仙米;化学成分;营养成分;生理活性;抗氧化;免疫调节;抗肿瘤;胆固醇代谢
摘要：
葛仙米是一种含有多种活性物质且具有各种生理活性的新食品原料。该文对葛仙米的主要化学成分和活性物质,及其在抗氧化、抑菌消炎、增强免疫、抑制肿瘤、调节胆固醇代谢以及抗凝血等方面的生理活性进行综述,并在此基础之上探讨葛仙米今后的研究方向与应用前景,为葛仙米的进一步深度合理开发利用提供重要参考。
语种：
中文
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嘌呤能受体P2X3的调控机理
作者：
徐圣尧;李彬;卢向阳;田云
期刊：
生命科学 ,2019年31(8):812-818 ISSN：1004-0374
作者机构：
湖南农业大学生物科学技术学院,长沙,410128;[田云; 李彬; 卢向阳; 徐圣尧] 湖南农业大学
关键词：
P2X3受体;激活剂;抑制剂
摘要：
P2X受体是由胞外ATP激活的配体型门控阳离子通道,其亚型P2X3受体在咳嗽、疼痛、高血压等疾病的发生过程中发挥重要作用,以P2X3受体为药物靶标开展研究对疾病的治疗有重要意义。该文就P2X3受体的结构、激活剂及抑制剂的作用机理展开综述。
语种：
中文
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Novel orange–red emitting phosphor Sr8ZnY(PO4)7:Sm3+ with enhanced emission based on Mg2+ and Al3+ incorporation for plant growth LED lighting
作者：
Zhou, Nan;Gao, Peixing;Yang, Yimei;Zhong, Yuan* ;Xia, Mao;...
期刊：
Journal of the Taiwan Institute of Chemical Engineers ,2019年104:360-368 ISSN：1876-1070
通讯作者：
Zhong, Yuan;Zhou, Zhi
作者机构：
[Xia, Mao; Zhong, Yuan; Zhou, Zhi; Zhang, Ye; Yang, Yimei; Zhou, Nan; Gao, Peixing] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Xia, Mao; Zhang, Ye; Yang, Yimei; Zhong, Yuan; Zhou, Nan; Gao, Peixing; Zhou, Zhi] Hunan Prov Engn Technol Res Ctr Opt Agr, Changsha 410128, Hunan, Peoples R China.;[Tian, Yun; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhong, Y; Zhou, Z] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
Activation energy;Citrus fruits;Emission spectroscopy;High temperature applications;Light emitting diodes;Phosphors;Solid state reactions;Thermodynamic stability;Chromaticity coordinates;Concentration quenching;Concentration-dependent;Diffuse reflection spectra;Excitation and emission spectra;High temperature solid-state reaction;Internal quantum efficiency;Plant growth;Light emission
摘要：
In this paper, a series of novel orange-red emission phosphors Sr8ZnY(PO4)(7):Sm3+ (SZYP:Sm3+) were synthesized by high-temperature solid-state reaction. The photoluminescence and concentration-dependent properties of this phosphor were investigated carefully through diffuse reflection spectra, excitation and emission spectra, Commission Internationale de l'Eclairage (CIE) chromaticity coordinate and decay times. SZYP:Sm3+ could be efficiently excited by n-UV and blue lights which center at 318, 345, 363, 376, 401, 410, 440 and 475 nm, respectively. The characteristic orange-red emission peaking at 563, 601 and 647 nm had been detected, which should be attributed to the characteristic f-f forbidden transition of Sm3+. The internal quantum efficiency (IQE) of ideal phosphor of SZYP:0.09Sm(3+) reached as high as 47%. In addition, The concentration quenching mechanism and thermal stability of SZYP:Sm3+ were further investigated. It was found SZYP:Sm3+ can maintain 93% of initial emission intensity at 150 degrees C and the corresponding activation energy was calculated to be 0.26 eV based on Arrhenius formula. The emission intensity of 1.0Mg(2+) and 0.02Al(3+) incorporated SZYP:0.09Sm(3+) reached up to 134% and 117% of the initial value, respectively. Moreover, (light-emitting diodes) LED devices were fabricated by employing the optimized samples as orange-red component with 365 nm n-UV chips. The desired emission in plant absorption region and extremely high thermal stability imply that SZYP:Sm3+ is suitable for the application in plant growth LED lighting. (C) 2019 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
语种：
英文
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不同光质对翡翠绿葛仙米中色素含量的影响
作者：
王卫;田云
期刊：
食品科技 ,2019年44(11):97-102 ISSN：1005-9989
作者机构：
湖南农业大学生物科学技术学院,长沙410128;常德炎帝生物科技有限公司,常德415000;[王卫; 田云] 湖南农业大学
关键词：
翡翠绿葛仙米;色素;光质
摘要：
翡翠绿葛仙米是一种葛仙米新种质,为了探讨光质对翡翠绿葛仙米中色素含量的影响,比较分析6种不同光质培养条件下翡翠绿葛仙米中各种色素的含量。结果表明:不同光质对翡翠绿葛仙米的色素含量均有影响,翡翠绿葛仙米的色素含量具有明显尺度效应,绿光促进叶绿素a的合成,蓝光促进藻红蛋白、类胡萝卜素的合成,红橙光、红光促进别藻蓝蛋白的合成,藻蓝蛋白的合成和藻胆蛋白总含量的光促规律受群体粒径的影响。因此,开展翡翠绿葛仙米规模化培养,需要关注不同粒径葛仙米的色素含量对光质的响应。
语种：
中文
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Improved Production of Arachidonic Acid by Combined Pathway Engineering and Synthetic Enzyme Fusion in Yarrowia lipolytica
作者：
Liu, Hu-Hu;Wang, Chong;Lu, Xiang-Yang;Huang, He;Tian, Yun* ;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2019年67(35):9851-9857 ISSN：0021-8561
通讯作者：
Tian, Yun;Ji, Xiao-Jun
作者机构：
[Tian, Yun; Liu, Hu-Hu; Huang, He; Wang, Chong; Lu, Xiang-Yang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Huang, He; Ji, Xiao-Jun] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China.
通讯机构：
[Tian, Yun] H;[Ji, Xiao-Jun] N;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China.
关键词：
Enzymes;Genetic engineering;Polyunsaturated fatty acids;Arachidonic acids;Isochrysis galbana;Pathway engineerings;Protein engineering;Special functions;Synthetic enzymes;Y. lipolytica;Yarrowia lipolytica;Biochemical engineering;acyl coenzyme A desaturase;acyltransferase;arachidonic acid;fatty acid elongases;glucose;biosynthesis;enzymology;genetics;Haptophyta;metabolic engineering;metabolism;Yarrowia;Acetyltransferases;Arachidonic Acid;Fatty Acid Desaturases;Glucose;Haptophyta;Metabolic Engineering;Yarrowia
摘要：
Arachidonic acid (ARA, C20:4) is a typical ω-6 polyunsaturated fatty acid with special functions. Using Yarrowia lipolytica as an unconventional chassis, we previously showed the performance of the Î""-6 pathway in ARA production. However, a significant increase in the Î""-9 pathway has rarely been reported. Herein, the Î""-9 pathway from Isochrysis galbana was constructed via pathway engineering, allowing us to synthesize ARA at 91.5 mg L-1. To further improve the ARA titer, novel enzyme fusions of Î""-9 elongase and Î""-8 desaturase were redesigned in special combinations containing different linkers. Finally, with the integrated pathway engineering and synthetic enzyme fusion, a 29% increase in the ARA titer, up to 118.1 mg/L, was achieved using the reconstructed strain RH-4 that harbors the rigid linker (GGGGS). The results show that the combined pathway and protein engineering can significantly facilitate applications of Y. lipolytica. © 2019 American Chemical Society.
语种：
英文
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黄瓜性别决定研究进展
作者：
周赓;陈宸;刘晓虹;卢向阳;田云;...
期刊：
植物生理与分子生物学学报 ,2019年55(7):902-914 ISSN：1671-3877
通讯作者：
Tian, Y.
作者机构：
湖南大学研究生院隆平分院,长沙410125;湖南省农业科学院,湖南省蔬菜研究所,长沙410125;[田云; 卢向阳; 陈宸] 湖南农业大学生物科学技术学院,长沙,410125;[陈惠明; 周赓] 湖南大学;[刘晓虹] 湖南省蔬菜研究所
通讯机构：
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
关键词：
黄瓜;性别决定;性别决定基因表达;激素调控
摘要：
黄瓜(Cucumis sativus)是进行性别研究的主要模式植物之一。性别决定机制的研究对于提高黄瓜雌花结实的产量具有重要意义。研究表明黄瓜植株的性别主要受黄瓜性别决定基因、环境条件和植物激素的影响。本文综述了近年来黄瓜性别决定的研究进展,并对黄瓜单性花发育机制以及黄瓜性别决定基因的时空表达提出了展望。
语种：
中文
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绿色荧光蛋白在枯草芽孢杆菌WB600中的表达
作者：
雍婕;杨辉;肖红仕;田云;周海燕
期刊：
合成生物学 ,2019年(5):71-75 ISSN：2096-8280
作者机构：
湖南农业大学生物科学技术学院,长沙,410128;[田云; 周海燕] 湖南农业大学生物科学技术学院,长沙 410128;[田云; 周海燕] 湖南省农业生物工程研究所,长沙 410128;[肖红仕; 杨辉; 雍婕] 湖南农业大学
关键词：
芽孢杆菌WB600;荧光蛋白;蛋白表达体系
摘要：
根据绿色荧光蛋白（green fluorescent protein,GFP）的特性,将GFP作为目的基因,与穿梭载体p HY300PLK结合,构建一个芽孢杆菌WB600的蛋白表达体系,为在枯草芽孢杆菌中表达其他蛋白提供了一定的技术和理论支持。本文结合p HY300PLK的克隆位点,设计gfp基因的引物,PCR扩增后回收的gfp基因片段进行双酶切,与相同双酶切的pHY300PLK连接获得pHY300PLK-GFP,通过电转化将pHY300PLK-GFP转化至芽孢杆菌WB600中并检测。获得转化子WB1,检测到转化子在荧光显微镜下显示绿色荧光,且后续测序检测表明重组质粒构建成功。
语种：
中文
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葛仙米跑道池规模化培养技术
作者：
易思富;田云
期刊：
现代农业科技 ,2019年(16):79-80,82 ISSN：1007-5739
作者机构：
湖南农业大学生物科学技术学院,湖南长沙 410128;常德炎帝生物科技有限公司;[田云; 易思富] 湖南农业大学
关键词：
葛仙米;淡水微藻;跑道池;培养技术
摘要：
葛仙米是一种营养丰富、开发应用价值较高的淡水微藻。其对生长环境要求很苛刻,野生分布区域狭窄,多年来人工培养技术主要采用塑料桶和玻璃缸等简单方式。本文介绍了一种跑道池培养葛仙米的规模化生产技术,以期为实现葛仙米产业化生产奠定基础。
语种：
中文
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分生孢子杂交配对筛选高价值蛹虫草新菌株试验
作者：
阳武雄;田云
期刊：
现代农业科技 ,2019年(4):50-52 ISSN：1007-5739
作者机构：
[阳武雄] 湖南农业大学生物科学技术学院,湖南长沙 410128;[阳武雄] 常德炎帝生物科技有限公司;湖南农业大学生物科学技术学院,湖南长沙,410128;[田云] 湖南农业大学
关键词：
蛹虫草;分生孢子;杂交配对;虫草素
摘要：
从退化菌株中筛选出具有原有特性的新菌株,对于蛹虫草的稳定生产具有重要的实际意义。本文首先从退化菌株中分离筛选出高活性的纯分生孢子,然后按照不同亲本、两两对峙的方式进行杂交选育,最终选育出2株高价值菌株（A5×B2和A5×B5）。其中,A5×B2菌株所产子实体中虫草素含量0.83%,子实体高度4.3 cm,干品单产5.25 g/瓶,外观评级"好";A5×B5菌株所产子实体中虫草素含量0.62%,子实体高度5.6 cm,干品单产6.01 g/瓶,外观评级"最好"。
语种：
中文
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