关键词：
Soil organic matter;Spatial prediction;Digital soil mapping;Sampling density optimization;Random forest
摘要：
Purpose Spatial prediction of soil organic matter (SOM) in cultivated land is crucial for evaluating soil productivity and its role in terrestrial carbon cycling. Cultivated soils in mountainous regions are commonly scattered on the footslope whereas those in the plain regions are continuously planar distributed; hence, they are quite different in the degree of variation in soil-forming factors and thereby the soil properties including SOM. Materials and methods In this study, we used the digital soil mapping approach (DSM) to predict SOM (0-20 cm) in cultivated soils in a hill-mountain region, Longshan County (LS), and a plain-platform region, Nanxian County (NX), which are both located at the same latitude in Southern China. By using 6746 and 9571 soil sampling points for LS and NX, respectively, together with 33 environmental covariates, the optimal spatial interpolation models and the reasonable sample strategy were carefully discussed. Results and discussion Descriptive statistical results showed that SOM in LS and NX were both moderate variations (coefficient variation, 0.34) and were approximately normal distribution. SOM in NX was strongly spatially dependent while SOM in LS was a moderate spatial dependence. The conditional Latin hypercube sampling (cLHS) was more appropriate compared with the Simple Random Sampling (SRS) as the sampling strategy. The optimal model for predicting cultivated land SOM was the Random Forest (RF) model for both LS and NX. The prediction accuracy was positively correlated with the sampling density. Specifically, to obtain a high prediction accuracy, the reasonable sampling density for SOM in LS should be controlled at >= 4.0 per km(2), higher than that in NX (>= 2.0 per km(2)). Conclusions The combination of cLHS and the RF model probably is the best choice for cultivated land SOM spatial prediction in different terrains. Therefore, our results provide a basis for future DSM of SOM in similar regions and help optimize soil sampling density.
摘要：
center dot Sugar transporter proteins (STPs) play critical roles in regulating plant stress tolerance, growth, and development. However, the role of STPs in regulating crop yield is poorly understood.center dot This study elucidates the mechanism by which knockout of the sugar transporter OsSTP15 enhances grain yield via increasing the tiller number in rice.center dot We found that OsSTP15 is specifically expressed in the shoot base and vascular bundle sheath of seedlings and encodes a plasma membrane-localized high-affinity glucose efflux transporter. OsSTP15 knockout enhanced sucrose and trehalose-6-phosphate (Tre6P) synthesis in leaves and improved sucrose transport to the shoot base by inducing the expression of sucrose transporters. Higher glucose, sucrose, and Tre6P contents were observed at the shoot base of stp15 plants. Transcriptome and metabolome analyses of the shoot base demonstrated that OsSTP15 knockout upregulated the expression of cytokinin (CK) synthesis-and signaling pathway-related genes and increased CK levels.center dot These findings suggest that OsSTP15 knockout represses glucose export from the cytoplasm and simultaneously enhances sugar transport from source leaves to the shoot base by promoting the synthesis of sucrose and Tre6P in leaves. Subsequent accumulation of glucose, sucrose, and Tre6P in the shoot base promotes tillering by stimulating the CK signaling pathway.
摘要：
Background: Brassinosteroids (BRs) are a class of naturally occurring steroidal phytohormones mediating a wide range of pivotal developmental and physiological functions throughout the plant’s life cycle. Therefore, it is of great significance to determine the content and the distribution of BRs in plants.Regretfully, although a large number of quantitative methods for BRs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been reported, the in planta distribution of BRs is still unclear because of their lower contents in plant tissues and the lack of effective ionizable groups in their chemical structures. Methods: We stablished a novel analytical method of BRs based on C18 cartridge solid-phase extraction (SPE) purification, 4-(dimethylamino)-phenylboronic acid (DMAPBA) derivatization, and online valve-switching system coupled with ultra-high performance liquid chromatography-electro spray ionization-triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS). This method has been used to quantify three structural types of BRs (epibrassinolide, epicastasterone, and 6-deoxo-24-epicastaster one) in different organs of Brassica napus L. (rapeseed). Results: We obtained the contents of three structural types of BRs in various organ tissues of rapeseed. The contents of three BRs in rapeseed flowers were the highest, followed by tender pods. The levels of three BRs all decreased during the maturation of the organs. We outlined the spatial distribution maps of three BRs in rapeseed based on these results, so as to understand the spatial distribution of BRs at the visual level. Conclusions: Our results provided useful information for the precise in situ localization of BRs in plants and the metabolomic research of BRs in future work. The in planta spatial distribution of BRs at the visual level has been studied for the first time.
摘要：
Lemon essential oil (LEO) is a common natural antibacterial substance, and encapsulating LEO into nanoemulsions (NEs) can improve their stability and broaden its application. Our study aimed to investigate the bacterial inhibitory effect of LEO-NEs against Escherichia coli (E. coli). Results showed that the minimum inhibitory concentration (MIC) of LEO-NEs was 6.25mg/mL, and the time-kill curve showed that E. coli were significantly killed by LEO-NEs after 5h of treatment at 1MIC. Flow-cytometry analysis showed that LEO-NEs adversely affected the cell-membrane depolarisation, cell-membrane integrity, and efflux pump function of E. coli. Confocal laser scanning microscopy demonstrated that 8MIC of LEO-NEs induced changes in the cell-membrane permeability and cell-wall integrity of E. coli. Proteomic results suggested that the mode of action LEO-NEs against E. coli was to enhance bacterial chemotaxis and significantly inhibit ribosomal assembly. They may also affect butyric acid, ascorbic acid and aldehyde metabolism, and sulphur-relay system pathways. In conclusion, LEO-NEs had potential application as a natural antibacterial agent for the control of E. coli in the food industry.
通讯机构：
[Li, T ; Qin, S ] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Peoples R China.
关键词：
crocin;Gardenia jasminoides Ellis;glycolipid metabolism disorder;gut microbiota;TLR4/Myd88/NF-kappa B pathway
摘要：
Gardenia jasminoides Ellis is abundant in crocin and has a longstanding historical usage both as a dietary and natural ethnic medicine. Enhanced studies have increasingly revealed the intricate interplay between glycolipid metabolism and gut microbiota, wherein their imbalance is regarded as a pivotal indicator of metabolic disorders. Currently, the precise molecular mechanism of the crude extract of crocin from Gardenia jasminoides Ellis (GC) targeting gut microbiota to regulate glycolipid metabolism disorder is still unclear. Firstly, we explored the effect of GC on digestive enzymes (alpha-amylase and alpha-glucosidase) in vitro. Secondly, we investigated the effect of GC on the physical and chemical parameters of high-fat diet (HFD) rats, such as body weight change, fasting blood glucose and lipid levels, and liver oxidative stress and injury. Then, 16S rDNA sequencing was used to analyze the effects of GC on the composition and structure of gut microbiota. Finally, the impact of GC on the TLR4/Myd88/NF-kappa B signaling pathway in the intestine was assessed by Western Blotting. In the present study, GC was found to exhibit a hypoglycemic effect in vitro, by inhibition of digestive enzymes. In animal experiments, we observed that GC significantly reduced fasting blood glucose, TC, and TG levels while increasing HDL-C levels. Additionally, GC demonstrated hepatoprotective properties by enhancing liver antioxidative capacity through the upregulation of SOD, CAT, and GSH-Px, while reducing ROS. 16S rDNA sequencing results showed that GC had a significant effect on the gut microbiota of HFD rats, mainly by reducing the ratio of Firmicutes/Bateroidota, and significantly affected the genera related to glycolipid metabolism, such as Akkermansia, Ligilactobacillus, Lactobacillus, Bacteroides, Prevotellaceae, etc. The Western Blotting results demonstrated that GC effectively downregulated the protein expressions of TLR4, Myd88, and NF-kappa B in the intestine of HFD rats, indicating that GC could target the TLR4/Myd88/NF-kappa B pathway to interfere with glycolipid metabolism disorder. Correlation analysis revealed that GC could target the Akkermansia-TLR4/Myd88/NF-kappa B pathway axis which attenuates glycolipid metabolism disorder. Therefore, this study establishes the foundation for GC as a novel therapeutic agent for glycolipid metabolism disorder chemoprevention, and it introduces a novel methodology for harnessing the potential of natural botanical extracts in the prevention and treatment of metabolic syndrome.
作者：
Li, Yiman;Henke, Michael;Zhang, Dalong;Wang, Chuanqing;Wei, Min
期刊：
Agronomy,2024年14(2):314- ISSN：2073-4395
通讯作者：
Wei, M
作者机构：
[Zhang, Dalong; Wei, M; Wang, Chuanqing; Wei, Min; Li, Yiman] Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Peoples R China.;[Henke, Michael] Hunan Agr Univ, Dept Smart Agr, Changsha 410128, Peoples R China.;[Zhang, Dalong; Wei, M; Wei, Min] China Japan Protected Hort Cooperat Res Ctr Shando, Tai An 271018, Peoples R China.
通讯机构：
[Wei, M ] S;Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Peoples R China.;China Japan Protected Hort Cooperat Res Ctr Shando, Tai An 271018, Peoples R China.
摘要：
Experimental studies were conducted on the cultivation of tomatoes (Solanum lycopersicum L.) at Shandong Agricultural University, China, from 2022 to 2023. Three cultivation patterns were designed as follows: a north–south orientation with a row spacing of 1.40 m (NS-1.4m), a north–south orientation with a row spacing of 1.80 m (NS-1.8m) and an east–west orientation with a row spacing of 1.80 m (EW-1.8m). A functional–structural plant model using the open source interactive modeling platform of GroIMP was constructed for the cultivation of tomatoes. The growth of plants as well as the light distribution and light interception capacity of the crop canopy were simulated and analyzed. The impacts of these cultivation patterns on the growth, photosynthetic characteristics, fruit ripening time, quality and yield of tomato plants were analyzed. The studies revealed that compared with the NS-1.4m treatment, the canopy light interception of tomato plants under the NS-1.8m and EW-1.8m treatments increased by 6.08% and 9.80% in a winter–spring crop and 6.80% and 19.76% in an autumn–winter crop, respectively. Their plant height, leaf area, aboveground dry matter accumulation, leaf net photosynthesis rate as well as the lycopene, vitamin C and sugar–acid ratio of the fruit all exhibited increasing trends, while fruit ripening was accelerated. The yield of the NS-1.8m and EW-1.8m treatments increased by 3.92% and 6.18% in a winter–spring crop and 4.17% and 9.78% in an autumn–winter crop, respectively. Structural equation modeling was used to further analyze the data, confirming that the cultivation of an east–west orientation with wide row spacing is beneficial for tomato cultivation in Chinese solar greenhouses. This cultivation pattern maximizes the canopy’s light interception, thus leading to improved fruit quality and yield. Overall, this study provides valuable insights for optimizing the cultivation pattern of solar greenhouse crops.
摘要：
Increased planting densities boost crop yields. A compact plant architecture facilitates dense planting. However, the mechanisms regulating compact plant architecture in cucurbits remain unclear. In this study, we identified a cucumber (Cucumis sativus) compact plant architecture (cpa1) mutant from an ethyl methane sulfonate (EMS)-mutagenized library that exhibited distinctive phenotypic traits, including reduced leaf petiole angle and leaf size. The candidate mutation causes a premature stop codon in CsaV3_1G036420, which shares similarity to Arabidopsis HOOKLESS 1 (HLS1) encoding putative histone N-acetyltransferase (HAT) protein and was named CsHLS1. Consistent with the mutant phenotype, CsHLS1 was predominantly expressed in leaf petiole bases and leaves. Constitutive overexpressing CsHLS1 in cpa1 restored the wild-type plant architecture. Knockout of CsHLS1 resulted in reduces leaf petiole angle and leaf size and as well as decreased acetylation levels. Furthermore, CsHLS1 directly interacted with CsSCL28 and negatively regulated compact plant architecture in cucumber. Importantly, CsHLS1 knockout increased the photosynthesis rate and leaf nitrogen in cucumbers, thereby maintaining cucumber yield at normal density. Overall, our research provides valuable genetic breeding resource and gene target for creating a compact plant architecture for dense cucumber planting.
摘要：
The distribution of nutrients in sediments is the result of multiple factors, including hydrological conditions and vegetation regulation, and in wetlands with complex hydrological conditions, this distribution is uncertain. In this study, the spatial distribution patterns of nitrogen and phosphorus in sediments were studied in the riverside and lakeside wetlands of Dongting Lake, a typical river-connected lake. The results showed that the nitrogen and phosphorus concentrations in the surface sediments were higher than those in the subsurface sediments in both the riverside and lakeside wetlands. In addition, the concentration of total nitrogen (TN) of lakeside wetlands in the surface sediments was higher than that of riverside wetlands, whereas the concentration of total phosphorus (TP) did not differ between the two wetland types. In the surface sediments, there were significant positive correlations between the TN and TP concentrations in the riverside wetlands (p < 0.05), but no significant correlation in the lakeside wetlands (p > 0.05). In riverside wetlands, the nitrogen and phosphorus concentrations showed strong spatial dependence. However, in the lakeside wetlands, the spatial dependence of NO3−-N was strong, that of NH4+-N was moderate, and that of TN and TP was weak. This study shows that both hydrology and vegetation cause differences in the distribution of nutrients in the sediments. The results obtained from this investigation clarify the differences of sediment nitrogen and phosphorus distributions in the two types of wetlands and provide a technical reference for the management of different wetland types.
期刊：
Journal of Traditional and Complementary Medicine,2024年 ISSN：2225-4110
通讯作者：
Ji Wang
作者机构：
[Xiangyan Liu; Ran Tao; Fangrui Guo; Linyu Zhang; Jianyu Qu; Mengyao Li; Xiaoran Wu; Yuanyuan Zhu; Lixin Wen] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China;[Xianglin Wang] Changsha Lvye Biotechnology Co., Ltd., Changsha, 410100, China;Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Ji Wang] Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Ji Wang] H;Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China
摘要：
Neuroinflammation is considered the principal pathogenic mechanism underlying neurodegenerative diseases, and the incidence of brain disorders is closely linked to dietary fat consumption and intestinal health. To investigate this relationship, 60 8-week-old C57BL/6J mice were subjected to a 20-week dietary intervention, wherein they were fed lard and soybean oil, each at 15% and 35% fat energy. At a dietary fat energy level of 35%, inflammation was observed in both the soybean oil and lard groups. Nevertheless, inflammation was more pronounced in the mice that were administered soybean oil. The process by which nerve cell structure is compromised, inflammatory factors are upregulated, brain antioxidant capacity is diminished, and the TLR4/MyD88/NF-κB p65 inflammatory pathway is activated resulting in damage to the brain-gut barrier. This, in turn, leads to a reduction in the abundance of Akkermansia and unclassified_f_Lachnospiraceae, as well as an increase in Dubosiella abundance, ultimately resulting in brain inflammation and damage. These results suggested that soybean oil induces more severe neuroinflammation compared to lard. Our study demonstrated that, at a dietary fat energy level of 35%, compared to soybean oil, lard could be the healthier option, the outcomes would help provide a reference basis for the selection of residents’ daily dietary oil.
作者机构：
[Lu, Ying; Zheng, Yajie; Xiao, Tian; Yang, Li; Nie, Guang] National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;[Xiao, Tian; Yang, Li; Nie, Guang] College of Horticulture, Hunan Agricultural University, Changsha 410128, China;[Zhong, Xiaohong; Yang, Fan] College of Horticulture, Hunan Agricultural University, Changsha 410128, China;[Jin, Xiue] Hubei Institute of Veterinary Drug Control, Wuhan 430000, China;[Peng, Xiaoying] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Lu, Ying; Zheng, Yajie] C;[Wang, Jun] H;Hubei Institute of Veterinary Drug Control, Wuhan 430000, China. Electronic address:;College of Horticulture, Hunan Agricultural University, Changsha 410128, China. Electronic address:
摘要：
The object of this study was to trace TwHf-derived toxins in raw honey and clarify their acute toxic effect related to the addition of honey or sugars. TwHf flowers, raw honey from TwHf planting base and from beekeepers in high-risk area were detected using LC-MS/MS. The results revealed five target toxins were detected in TwHf flowers; only celastrol was detected in one raw honey sample, as a food safety risk factor, celastrol had been traced back to TwHf flowers from raw honey. In a series of acute toxic tests on zebrafish, toxification effects were observed when honey, mimic honey or sugar was mixed with toxins. The degree of toxicity varied among various sugar-based solutions. At the same mass concentration, they follow this order: raw honey/mimic honey > glucose > fructose. The main toxic target organs of triptolide and celastrol with honey were the heart and liver.
摘要：
We investigated the current status and main factors of loneliness among rural left-behind children. We conducted a survey of 812 rural left-behind children in China using the Sociodemographic Questionnaire, the Children's Loneliness Scale, the Social Anxiety Scale for Children, the Rosenberg's Self-Esteem Scale, and the Piers-Harris Child Self-Concept Scale. Results showed that (a) 66.26% had a sense of loneliness; (b) there were correlations between loneliness and 7 sociodemographic variables, social anxiety, self-esteem, and self-concept; (c) grade, age, parental separation duration, contact frequency with parents, social anxiety, self-esteem, and self-concept were the main factors influencing the level of loneliness among rural left-behind children. Recommendations for parents and teachers are suggested.
