期刊：
Global Ecology and Conservation,2021年27:e01535 ISSN：2351-9894
通讯作者：
Yeai Zou<&wdkj&>Yonghong Xie
作者机构：
[Chen, Xinsheng; Deng, Zhengmiao; Zhang, Siqi; Zou, Yeai; Zhu, Feng; Li, Feng; Xie, Yonghong; Zhang, Pingyang] Chinese Acad Sci, Key Lab Agr Proc Subtrop Reg, Changsha 410125, Peoples R China.;[Chen, Xinsheng; Deng, Zhengmiao; Zhang, Siqi; Zou, Yeai; Li, Feng; Xie, Yonghong; Zhang, Pingyang] Chinese Acad Sci, Inst Subtrop Agr, Dongting Lake Stn Wetland Ecosyst Res, Changsha 410125, Peoples R China.;[Zhang, Siqi; Zhang, Pingyang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Pan, Baihan] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China.;[Zhu, Feng] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.
通讯机构：
[Yeai Zou; Yonghong Xie] K;Key Laboratory of Agro-ecological Processes in Subtropical Regions, Chinese Academy of Sciences, Changsha 410125, PR China<&wdkj&>Dongting Lake Station for Wetland Ecosystem Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, PR China<&wdkj&>Key Laboratory of Agro-ecological Processes in Subtropical Regions, Chinese Academy of Sciences, Changsha 410125, PR China<&wdkj&>Dongting Lake Station for Wetland Ecosystem Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, PR China
作者机构：
[Pan, Jiafeng] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Pan, Jiafeng; Chen, Junhua] Guangdong Acad Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut, Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Peoples R China.;[Bao, Enhu; Bao, Dongqin] Xuzhou Med Univ, Shuyang Hosp, Suqian 223800, Peoples R China.
摘要：
A simple, highly sensitive biosensor for S. aureus detection is becoming increasingly important in human health and safety. In this work, a hairpin probe-mediated DNA circuit for the detection of the mecA gene of S. aureus was reported cascading Exo III-assisted cycling signal amplification and the DNAzyme-mediated cleavage reaction. In the presence of the target mecA gene, the recognition and hybridization between HP1 and mecA can trigger Exo III and DNAzyme-mediated signal amplification and further release numerous ATMND, resulting in an enhanced fluorescence response, which serves as a response signal for the fluorescence detection of mecA gene. This biosensor enables the sensitive and specific detection of the mecA gene, showing a linear response ranging from 1 fM to 1 nM with a detection limit of 0.5 fM. Moreover, this fluorescence assay has been applied for the analysis of clinical samples with satisfactory recovery. Importantly, this universal platform can be further extended for the analysis of other targets by alternating the corresponding recognition unit, which holds much promise in point-of-care testing for bacterial analysis.
摘要：
The development of the low-cost, highly efficient, and environment-friendly electrocatalyst in hydrogen evolution reaction (HER) is important to solve the increasingly severe energy crisis and environmental pollution. In this work, the Cu3BiS3/copper foam (CF) was synthesized by a one-step hydrothermal method, where the unique flower-like structures formed with the Cu3BiS3 nanosheets were in situ grown on the 3D CF. The electrode of Cu3BiS3/CF exhibits an excellent HER performance with an onset potential of 273 mV and a Tafel slope of 143 mV dec−1. The control experiments show that the HER performance of Cu3BiS3 is better than that of its binary counterparts of Bi2S3 and Cu7S4. The improved electrocatalytic property of Cu3BiS3/CF may be attributed to its flower-like structures that increase the surface area and expose more active sites, and to the synergistic effect of the metal/metal sulfide. This work opens the way to facilely grow the ternary metal sulfides with the hierarchical nanostructure that has the potential in the field of HER electrocatalyst.
摘要：
This experiment was conducted to investigate the effects of different supplementation levels of palygorskite and probiotics complex (Pal-Pro) on laying performance, hatching performance, egg quality, plasma antioxidative status, and immune response of broiler breeders. A total of 432 Xuefeng black-bone chickens (42-week-old) were randomly assigned into four treatments with six replicates of 18 hens. The broiler breeders were fed the basal diet supplemented with 0, 250, 500, and 750 mg/kg Pal-Pro for 8 weeks. The Pal-Pro supplementation linearly or quadratically increased (p < .05) laying rate, fertilisation rate, hatchability of fertile eggs, heath hatched chicks rate, eggshell thickness, yolk index, total antioxidant capacity, activities of total superoxide dismutase, catalase, glutathione peroxidase, and contents of immunoglobulins, interleukin-2, and tumour necrosis factor-α in plasma. With Pal-Pro supplementation increased, feed convention ratio during 5–8 and 1–8 week, embryo mortality, malondialdehyde linearly decreased (p < .05). This finding suggested that dietary supplementation with 750 mg/kg Pal-Pro administration could improve the antioxidative status and immunoglobulins contents in plasma of broiler breeders, which may contribute to an increase in broiler’s laying rate, hatching performance, egg quality, such as eggshell thickness and yolk index.
Dietary supplementation of Pal-Pro decreased the feed conversion ratio and embryo mortality of broiler breeders.
Dietary supplementation of Pal-Pro improved the laying rate, fertility, hatchability of fertile eggs, and yolk index of broiler breeders.
Dietary supplementation of Pal-Pro enhanced the antioxidant capacity and the level of immunoglobulins in the plasma.
摘要：
From the way of free aid to the cooperation mode of aid, trade and investment, Sino African agricultural cooperation has made long-term progress. The modern agricultural production mode has accelerated the development of agricultural economy and greatly enriched people's material life. However, in the background of pursuing the rapid growth of agricultural economy, it also brings pollution and destruction to the rural ecological environment and threatens the long-term survival and development of people. Taking the Sino African cooperation project as an example, this paper qualitatively studies the current situation of agricultural economy and rural ecological environment in an African city, constructs the evaluation index system of agricultural economic system from two dimensions of economic strength and economic vitality, constructs the evaluation index system of rural ecological environment system from three dimensions of ecological quality, production environment and environmental pollution, and introduces the coefficient of variation measurement model. The coordinated development of agricultural economy and rural ecological environment in recent ten years is quantitatively evaluated. The results show that the overall development trend of agricultural economy and rural ecological environment in the study period is good. The degree of coordinated development is constantly improving, and the type of coordinated development transits from mild imbalance in the early stage to the verge of imbalance, and from the later stage to barely coordinated type.
期刊：
Journal of Biological Chemistry,2021年296:100655 ISSN：0021-9258
通讯作者：
Li, Changzhu;Yu, Ye;Wang, Jin
作者机构：
[Sun, Liang-Fei; Yu, Ye; Chen, Ping-Fang] Shanghai Jiao Tong Univ, Inst Med Sci, Dept Pharmacol & Chem Biol, Sch Med, Shanghai, Peoples R China.;[Guo, Chang-Run; Yu, Ye; Wang, Jin; Ma, Xue-Fei] China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing, Peoples R China.;[Tian, Yun; Ma, Xue-Fei] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.;[Fan, Ying-Zhe] Shanghai Univ Chinese Tradit Med, Putuo Hosp, Shanghai, Peoples R China.;[Li, Changzhu; Xiao, Zhihong; Li, Peiwang] Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, Changsha, Peoples R China.
通讯机构：
[Changzhu Li; Jin Wang] S;[Ye Yu] D;Department of Pharmacology and Chemical Biology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China<&wdkj&>School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China<&wdkj&>State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, China<&wdkj&>School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China<&wdkj&>State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha, China
摘要：
Highly conserved amino acids are generally anticipated to have similar functions across a protein superfamily, including that of the P2X ion channels, which are gated by extracellular ATP. However, whether and how these functions are conserved becomes less clear when neighboring amino acids are not conserved. Here, we investigate one such case, focused on the highly conserved residue from P2X4, E118 (rat P2X4 numbering, rP2X4), a P2X subtype associated with human neuropathic pain. When we compared the crystal structures of P2X4 with those of other P2X subtypes, including P2X3, P2X7 and AmP2X, we observed a slightly altered side-chain orientation of E118. We used protein chimeras, double mutant cycle analysis and molecular modeling to reveal that E118 forms specific contacts with amino acids in the "beak" region, which facilitates ATP binding to rP2X4. These contacts are not present in other subtypes due to sequence variance in the beak region, resulting in decoupling of this conserved residue from ATP recognition and/or channel gating of P2X receptors. Our study provides an example of a conserved residue with a specific role in functional proteins enabled by adjacent non-conserved residues. The unique role established by the E118-beak region contact provides a blueprint for the development of subtype-specific inhibitors of P2X4.
通讯机构：
[Xi He; Zhiyong Fan] E;Engineering Research Center for Feed Safety and Efficient Utilization of Ministry of Education, Institute of Animal Nutrition, Hunan Agricultural University, Hunan, 410128, China<&wdkj&>Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Hunan, 410128, China<&wdkj&>Engineering Research Center for Feed Safety and Efficient Utilization of Ministry of Education, Institute of Animal Nutrition, Hunan Agricultural University, Hunan, 410128, China<&wdkj&>Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Hunan, 410128, China
关键词：
Sow;Isomaltooligosaccharide;Bacillus;Perinatal period;Duration of farrowing;Gut microbiota
摘要：
This study investigated the effects of isomaltooligosaccharide (IMO) and Bacillus in perinatal diets on the duration of farrowing and post-weaning estrus, serum reproductive hormone concentrations, and gut microbiota and its metabolites of sows. Multiparous sows (n = 130) were fed diets without IMO (control, CON group), or diets containing only IMO (IMO group), IMO and Bacillus subtilis (IMOS group), IMO and Bacillus licheniformis (IMOL group), and IMO and B. subtilis and B. licheniformis (IMOSL group), respectively. The results indicate that the duration of farrowing and post-weaning estrus was shorter in sows in the IMOS, IMOL, and IMOSL groups, and the weaning-estrous interval was lower in sows in the IMOL greoup. In addition, the lowest fecal score was observed in the IMOL group during d 106 to 112 of gestation. Sows in most of the treatment groups had a higher concentration of serum prolactin and prostaglandin at farrowing, but a lower serum concentration of estradiol, oxytocin, and progesterone on d 18 of lactation than sows in the CON group. The treatment groups had a higher abundance of Candidatus Methanoplasma and Bacillus and a lower abundance of Escherichia-Shigella in their feces at farrowing. Furthermore, the treatment groups had higher concentrations of total short-chain fatty acids (SCFA) in feces at farrowing and a higher concentration of branched fatty acids in feces on d 18 of lactation. Furthermore, the abundance of Bacillus in feces was positively correlated with serum prostaglandin concentrations and fecal total SCFA of sows at farrowing, but was negatively correlated with the duration of farrowing. Overall, dietary IMO and Bacillus supplementation affected the concentration of serum reproductive hormones and the duration of farrowing and post-weaning estrus, and the gut microbiota is a key factor.
摘要：
Texture qualities and pectin characteristics in fermented minced pepper (FMP) prepared by natural fermentation (NF) and inoculated fermentation (IF) were analysed during fermentation. The results showed variation in texture qualities and pectin characteristics was similar during NF and IF process. The hardness, cell wall material, sodium carbonate-soluble pectin (SSP) and chelate-soluble pectin (CSP) content, and CSP esterification degree decreased, while water-soluble pectin (WSP) content significantly (P < 0.05) increased after fermentation. The rhamnose (Rha) molar ratio in three pectins increased, but arabinose (Ara) and galactose (Gal) molar ratios in most pectins decreased after fermentation. Changes in Ara/Gal and (Gal + Ara)/Rha ratios represented the backbone and branched chains of rhamnogalacturonan-I in three pectins depolymerised during fermentation. The decrease of molecular weight (M-w) in CSP was more obvious than that in WSP and SSP, and it was extensively depolymerised into low-M-w pectin after fermentation. Pearson's correlation analysis showed FMP hardness was extremely (P < 0.01) positively correlated with CSP content and significantly (P < 0.05) positively correlated with SSP content and CSP M-w. Hence, CSP was the main pectin to affect texture compared with WSP and SSP, and its characteristics played a crucial role for regulating FMP texture during NF and IF process.
摘要：
Gelsenicine is one of the most toxic compounds in the genus Gelsemium, but the mechanism of toxicity is not clear. In this paper, tandem mass tag quantitative phosphoproteomics was used to study the changes in protein phosphorylation in different brain regions at different time points after gelsenicine poisoning in mice. The correlation between neurotransmitter receptors and the toxicity of gelsenicine was analyzed by molecular docking and rescue experiments. Parallel reaction monitoring (PRM) was used to verify the related proteins. A total of 17877 unique phosphosites were quantified and mapped to 4170 brain proteins to understand the signaling pathways. Phosphoproteomics revealed gelsenicine poisoning mainly affected protein phosphorylation levels in the hippocampus, and through bioinformatics analysis, it was found gelsenicine poisoning significantly affected neurotransmitter synaptic pathway. The molecular docking results showed that gelsenicine could bind to the N-methyl-D-aspartic acid receptor (NMDAR). In addition, we found that NMDA was effective in improving the survival rate of the animals tested, and this effect was associated with reduced protein phosphorylation by PRM validation. The results revealed that gelsenicine affects neurotransmitter release and receptor function. This is the first demonstration that NMDA receptor-mediated excitotoxicity is a key signaling pathway in the toxicity of gelsenicine.
摘要：
As an aliphatic amino acid, cysteine (CYS) is diffuse in the living cells of plants and animals. However, little is known of its role in the reactivity of nano-sized zero-valent iron (NZVI) in the degradation of pollutants. This study shows that the introduction of CYS to the NZVI system can help improve the efficiency of reduction, with 30% more efficient degradation and a reaction rate constant nine times higher when nitrobenzene (NB) is used as probe compound. The rates of degradation of NB were positively correlated with the range of concentrations of CYS from 0 to 10 mmol/L. The introduction of CYS increased the maximum concentration of Fe(III) by 12 times and that of Fe(II) by four times in this system. A comparison of systems featuring only CYS or Fe(II) showed that the direct reduction of NB was not the main factor influencing its CYS-stimulated removal. The reduction in the concentration of CYS was accompanied by the generation of cystine (CY, the oxidized form of cysteine), and both eventually became stable. The introduction of CY also enhanced NB degradation due to NZVI, accompanied by the regeneration of CYS. This supports the claim that CYS can accelerate electron transfer from NZVI to NB, thus enhancing the efficiency of degradation of NB.
通讯机构：
[Jienan Chen] M;[Xiaoxun Zhou] C;College of Public Administration and Law, Human Agricultural University, Changsha, 410128, China<&wdkj&>Ministry of Forestry Bioethanol Research Center, Changsha, 410004, China<&wdkj&>Hunan International Joint Laboratory of Woody Biomass Conversion, Changsha, 410004, China<&wdkj&>College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China
摘要：
Biopolymer-based slow-release fertilizers (SRFs) have attracted increasing interest because of their environmental benefits. Herein, a hydrophobic thermoplastic lignin with favorable film formation properties was synthesized through simple crosslinking followed by esterification reactions. The chemical structure, glass transition temperature, and hydrophobicity were determined using Fourier transform infrared spectroscopy, H-1 nuclear magnetic resonance spectroscopy, differential scanning calorimetry, and contact angle studies. The newly synthesized esterified crosslinked sodium lignosulfonate (ECSL) enables the encapsulation of urea via a solvent-free coating method to prepare SRFs because of its appropriate glass transition temperature (Tg) and excellent film formation properties. The ECSL-based SRF released only 86.9% of the encapsulated urea within 44 d, which is superior to most lignin-based SRFs in previous studies. The release rate can also be tuned by adjusting the ECSL ratio. The synthesis of hydrophobic lignin biocomposites with suitable Tgs for film formation resolves the bottleneck of using these types of non-thermoplastic polymers (e.g., lignin) for SRFs. This study not only maximizes the value of biowaste lignin but also offers a non-paradigm approach toward low-cost SRFs for sustainable agriculture.
期刊：
Soil Biology and Biochemistry,2021年160:108355 ISSN：0038-0717
通讯作者：
Gengxin Zhang
作者机构：
[Chen, Qiuyu; Yang, Xiaoqin; Niu, Bin; Jiao, Hongzhe; Song, Lili; Hu, Yilun; Zhang, Gengxin; Xu-Ri] Chinese Acad Sci, Inst Tibetan Plateau Res, State Key Lab Tibetan Plateau Earth Syst Resource, Beijing 100101, Peoples R China.;[Chen, Qiuyu; Zhang, Gengxin] Hubei Univ, Fac Resources & Environm Sci, Hubei Key Lab Reg Dev & Environm Response, Wuhan 430062, Peoples R China.;[Hu, Ang] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.;[Yang, Xiaoqin; Niu, Bin; Jiao, Hongzhe] Univ Chinese Acad Sci, Beijing 100101, Peoples R China.
通讯机构：
[Gengxin Zhang] S;State Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100101, China<&wdkj&>Hubei Key Laboratory of Regional Development and Environmental Response, Faculty of Resources and Environmental Science, Hubei University, Wuhan, 430062, China
期刊：
Computers and Geotechnics,2021年137:104259 ISSN：0266-352X
通讯作者：
Chen, Xiaobin(chen_xiaobin@csu.edu.cn)
作者机构：
[Li, Ruidong; Yang, Ningyu; Chen, Xiaobin; Hu, Hang; Zhang, Jiasheng] Cent South Univ, Sch Civil Engn, Changsha 410083, Hunan, Peoples R China.;[Zhang, Junqi] Hunan Agr Univ, Coll Water Resources & Civil Engn, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiaobin Chen] S;[Junqi Zhang] C;College of Water Resources and Civil Engineering, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>School of Civil Engineering, Central South University, Changsha, Hunan 410083, China
关键词：
Discrete element method;Fiber stiffness;Fiber-reinforced soil;Micromechanical properties;Shear behavior
通讯作者：
Jun-Pei Yue<&wdkj&>Xiong-Wei Wu<&wdkj&>Qi Deng
作者机构：
[Zuo, Lan-Lan; Ma, Qiang; Liu, Xue-Jiao; Wu, Xiong-Wei; Chen, Meng-Jie; Fu, Sha] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Hunan, Peoples R China.;[Zhou, Peng-Sheng] Ningxia Univ, Sch Chem & Chem Engn, Yinchuan 750021, Ningxia, Peoples R China.;[Deng, Qi; Yue, Jun-Pei] Chinese Acad Sci, CAS Key Lab Mol Nanostruct & Nanotechnol, CAS Res Educ Ctr Excellence Mol Sci, Beijing Natl Lab Mol Sci BNLMS,Inst Chem, Beijing 100190, Peoples R China.;[Deng, Qi] Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource China, Changsha 41004, Hunan, Peoples R China.
通讯机构：
[Jun-Pei Yue; Qi Deng] C;[Xiong-Wei Wu] S;CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, P. R. China<&wdkj&>Hunan Academy of Forestry, State Key Laboratory of Utilization of Woody Oil Resource of China, Changsha, Hunan 41004, China<&wdkj&>School of Chemistry and Materials Science, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190, P. R. China
摘要：
Lithium-metal batteries (LMBs) as the next generation of promising high energy density alternatives among electrochemical storage technologies have received worldwide attention. However, the incompatibility between metallic lithium and traditional liquid electrolytes is the dominant reason for the formation of lithium dendrites, consequently leading to the deterioration of LMBs. Although the development of solid electrolytes such as inorganic electrolytes, composite polymer electrolytes, and polymer electrolytes solve the leakage problem of the toxic and volatile liquid electrolyte, the low ion migration in the bulk and the electrode-electrolytes at room temperature cannot guarantee continuous Li+ carrier transportation. Gel polymer electrolytes with a synergetic superiority of the solid electrolytes and liquid electrolytes are attractive for application in high energy density LMBs. In this review, the scientific challenges, ion transfer mechanisms, and design strategies for gel polymer electrolytes (GPEs) are presented. The design of functional gel polymer electrolytes, focusing on the thermal response behavior, metallic lithium anode and cathode in whole batteries, is summarized. The challenges and perspectives for the rational design of safe and functional gel polymer electrolytes towards high performance, and the assurance of safe LMBs, are also discussed.
通讯机构：
[Venkat Gopalan; Guo-Liang Wang] D;[Wende Liu] S;Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, Columbus, Ohio, USA<&wdkj&>Department of Plant Pathology, The Ohio State University, Columbus, OH, USA<&wdkj&>State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
摘要：
This study was conducted to examine the influences of replacing soybean meal (SBM) with fermented soybean meal (FSBM) in the diet of lactating Holstein cattle on rumen fermentation and ruminal bacterial microbiome. Twenty-four lactating Chinese Holstein dairy cattle were assigned to each of the two treatments in a completely randomized design: the SBM group [the basal total mixed ration (TMR) diet containing 5.77% SBM] and the FSBM group (the experimental TMR diet containing 5.55% FSBM). This trial lasted for 54 days (14 days for adjustment and 40 days for data and sample collection), and samples of rumen liquid were collected on 34 d and 54 d, respectively. The results showed that replacing SBM with FSBM significantly increased the molar percentages of propionate (P < 0.01) and valerate (P < 0.05), but reduced the total volatile fatty acid (TVFA) concentration (P < 0.05), butyrate molar proportion (P < 0.05), and the acetate to propionate ratio (P < 0.01). The copy numbers of total bacteria (P < 0.05), Fibrobacter succinogenes (P < 0.01), Selenomonas ruminantium (P < 0.01), and Prevotella spp. (P < 0.05) in the FSBM group were greater, while the density of Prevotella ruminicola (P < 0.05) was lower than those in the SBM treatment. Additionally, Succiniclasticum ruminis and Saccharofermentans acetigenes were significantly enriched (P < 0.05) in the rumen fluid of FSBM-fed cows, despite the fact that there was no remarkable difference in the Alpha diversity indexes, structure and KEGG pathway abundances of the bacterial community across the two treatments. It could hence be concluded that the substitution of FSBM for SBM modulated rumen fermentation and rumen bacterial microbiota in lactating Holstein dairy cows. Further research is required to elucidate the relevant mechanisms of FSBM, and provide more insights into the application of FSBM in dairy cattle.
