通讯机构：
[Zhili He [email protected]] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou, China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha, China
摘要：
Biodiversity is vital for ecosystem functions and services, and many studies have reported positive, negative, or neutral biodiversity-ecosystem functioning (BEF) relationships in plant and animal systems. However, if the BEF relationship exists and how it evolves remains elusive in microbial systems. Here, we selected 12 Shewanella denitrifiers to construct synthetic denitrifying communities (SDCs) with a richness gradient spanning 1 to 12 species, which were subjected to approximately 180 days (with 60 transfers) of experimental evolution with generational changes in community functions continuously tracked. A significant positive correlation was observed between community richness and functions, represented by productivity (biomass) and denitrification rate, however, such a positive correlation was transient, only significant in earlier days (0 to 60) during the evolution experiment (180 days). Also, we found that community functions generally increased throughout the evolution experiment. Furthermore, microbial community functions with lower richness exhibited greater increases than those with higher richness. Biodiversity effect analysis revealed positive BEF relationships largely attributable to complementary effects, which were more pronounced in communities with lower richness than those with higher richness. This study is one of the first studies that advances our understanding of BEF relationships and their evolutionary mechanisms in microbial systems, highlighting the crucial role of evolution in predicting the BEF relationship in microbial systems.IMPORTANCE Despite the consensus that biodiversity supports ecosystem functioning, not all experimental models of macro-organisms support this notion with positive, negative, or neutral biodiversity-ecosystem functioning (BEF) relationships reported. The fast-growing, metabolically versatile, and easy manipulation nature of microbial communities allows us to explore well the BEF relationship and further interrogate if the BEF relationship remains constant during long-term community evolution. Here, we constructed multiple synthetic denitrifying communities (SDCs) by randomly selecting species from a candidate pool of 12 Shewanella denitrifiers. These SDCs differ in species richness, spanning 1 to 12 species, and were monitored continuously for community functional shifts during approximately 180-day parallel cultivation. We demonstrated that the BEF relationship was dynamic with initially (day 0 to 60) greater productivity and denitrification among SDCs of higher richness. However, such pattern was reversed thereafter with greater productivity and denitrification increments in lower-richness SDCs, likely due to a greater accumulation of beneficial mutations during the experimental evolution. Despite the consensus that biodiversity supports ecosystem functioning, not all experimental models of macro-organisms support this notion with positive, negative, or neutral biodiversity-ecosystem functioning (BEF) relationships reported. The fast-growing, metabolically versatile, and easy manipulation nature of microbial communities allows us to explore well the BEF relationship and further interrogate if the BEF relationship remains constant during long-term community evolution.
通讯机构：
[Su, ZH ; Hu, SY; Zhao, Y ] H;Hunan Agr Univ, Coll Chem & Mat Sci, Coll Agron, Changsha 410128, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Peoples R China.
摘要：
Nicotine (NIC) is a harmful substance, drug, pesticide and chemical that is widely found in tobacco. It has carcinogenic, teratogenic and neurotoxic effects that have raised serious concerns. Herein, a colorimetric sensor with dual-ratio and dual-mode for the detection of NIC in tobacco samples was reported. The localized surface plasmon resonance signals of gold nanoparticles (AuNPs) and AuNPs-NIC are used as dual-ratio signals. The absorbance ratio of NIC to AuNPs or the absorbance ratio of NIC to AuNPs-NIC and the wavelength shift value of AuNPs-NIC are applied as dual-mode. Transmission electron microscopy, energy dispersive spectroscopy, dynamic light scattering spectroscopy, ultraviolet-visible spectrophotometry, cyclic voltammetry, and potentiostatic methods were used to characterize the sensor. Further analysis of NIC was conducted through morphological fitting and theoretical calculations. Under optimal conditions, the sensor shows a wide linear range of 5-500 mu M. The detection limits for NIC are 2.48 mu M, 1.63 mu M and 1.34 mu M, respectively. The experimental result shows that the dual-ratio signal of AuNPs and AuNPs-NIC has good selectivity and sensitivity, and can effectively reduce the interference of impurities on NIC detection. And the dual-mode of detection for NIC improves the accuracy and comparability of the result significantly. In addition, the proposed sensor was also applied to test NIC in tobacco samples with satisfactory recovery. Nicotine (NIC) is a harmful substance, drug, pesticide and chemical that is widely found in tobacco.
期刊：
Science of The Total Environment,2023年871:162153 ISSN：0048-9697
通讯作者：
Chen, Liang;Xie, Y
作者机构：
[Chen, Liang; Xie, Yan; Feng, Qijia; Wassie, Misganaw] Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Plant Germplasm Enhancement & Specialt, Wuhan Bot Garden, Wuhan 430074, Peoples R China.;[Chen, Liang; Xie, Yan; Feng, Qijia; Wassie, Misganaw] Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan 430074, Peoples R China.;[Feng, Qijia; Wassie, Misganaw] Univ Chinese Acad Sci, Sch Life Sci, Beijing, Peoples R China.;[Cao, Shilong; Liao, Shujie] Hunan Agr Univ, Coll Agron, Dept Pratacultural Sci, Changsha 410128, Peoples R China.;[Sun, Xiaoyan] Jiangxi Acad Sci, Inst Microbe, Jiangxi Engn & Technol Res Ctr Ecol Remediat Heavy, Nanchang 330096, Peoples R China.
通讯机构：
[Chen, L; Xie, Y ] C;Chinese Acad Sci, Innovat Acad Seed Design, CAS Key Lab Plant Germplasm Enhancement & Specialt, Wuhan Bot Garden, Wuhan 430074, Peoples R China.
关键词：
Fusarium equiseti;Perennial ryegrass;Plant growth-promoting fungi;Salt tolerance;Soil microbial community
摘要：
Fusarium equiseti is an effective plant growth-promoting fungi that induce systemic disease resistance in plants. However, the role of F. equiseti in regulating salt stress response and the underlying mechanisms remain largely unknown. Here, we investigated the effect of F. equiseti Z7 strain on the growth and salt stress response in perennial ryegrass. Additionally, the role of Z7 in regulating the abundance, composition, and structure of native microbial communities in the rhizosphere soil was determined. We observed that Z7 could produce indole-3-acetic acid (IAA) and siderophores. Hence, Z7 inoculation further enhanced plant growth and salt tolerance in perennial ryegrass. Inoculating Z7 increased K(+) and decreased Na(+) in plant tissues. Z7 inoculation also enhanced soil quality by reducing soluble salt and increasing available phosphorus. Moreover, inoculating Z7 altered the compositions of bacterial and fungal communities in the rhizosphere soil. For instance, beneficial bacterial genera, such as Flavobacterium, Enterobacter, Agrobacterium, and Burkholderiales were dominantly enriched in Z7-inoculated soil. Interestingly, the relative abundance of these genera showed significantly positive correlations with the fresh weight of perennial ryegrass. Our results demonstrate that Z7 could remarkably promote plant growth and salt tolerance by regulating ion homeostasis in plant tissues and microbial communities in the rhizosphere soil. This study provides a scientific foundation for applying microbes to improve plant growth under extreme salt stress conditions.
作者机构：
[Li, Yuchen; Chen, Long; Mi, Baobin; He, Jiangnan; Wu, Fangfang; Zhou, Zhi] Hunan Agr Univ, Coll Agron, Coll Biosci & Biotechnol, Hunan Engn Res Ctr Biochar,Sch Chem & Mat Sci, Changsha 410128, Hunan, Peoples R China.;[Mi, Baobin] Hunan Acad Agr Sci, Res Inst Vegetables, Changsha 410125, Peoples R China.
通讯机构：
[Fangfang Wu] S;School of Chemistry and Materials Science, Hunan Engineering Research Center for Biochar, College of Agronomy, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China
摘要：
In this study, functionalized banana peel biochar (BPB) was prepared by microwave-assisted pyrolysis for the first time to investigate its adsorption to malachite green (MG) dye. Adsorption experiments showed that the maximum adsorption capacity of BPB500 and BPB900 to malachite green reached 1790.30 and 2297.83 mg.g(-1) within 120 min. The adsorption behaviour was well-fitted by the pseudo-second-order kinetic model and Langmuir isotherm model, and Delta G(0) < 0, Delta H-0 > 0, indicated that the adsorption process was endothermic and spontaneous, dominated by chemisorption. The adsorption mechanism of MG dye on BPB included hydrophobic interaction, hydrogen bonding, pi-pi interaction, n-pi interaction, and ion exchange. Meanwhile, through regeneration tests, simulated wastewater treatment experiments, and cost calculations, it was found that BPB has great potential for practical applications. This work demonstrated that microwave-assisted pyrolysis is a viable low-cost approach for producing excellent sorbents from biomass, and banana peel is a promising feedstock to prepare biochar for dye removal.
摘要：
The receptor protein PEX5, an important component of peroxisomes, regulates growth, development, and immunity in yeast and mammals. PEX5 also influences growth and development in plants, but whether it participates in plant immunity has remained unclear. Here, we report that knockdown of OsPEX5 enhances resistance to the rice blast fungus Magnaporthe oryzae. We demonstrate that OsPEX5 interacts with the E3 ubiquitin ligase APIP6, a positive regulator of plant immunity. APIP6 ubiquitinates OsPEX5 invitro and promotes its degradation invivo via the 26S proteasome pathway. In addition, OsPEX5 interacts with the aldehyde dehydrogenase OsALDH2B1, which functions in growth-defense trade-offs in rice. OsPEX5 stabilizes OsALDH2B1 to enhance its repression of the defense-related gene OsAOS2. Our study thus uncovers a previously unrecognized hierarchical regulatory mechanism in which an E3 ubiquitin ligase targets a peroxisome receptor protein that negatively regulates immunity in rice by stabilizing an aldehyde dehydrogenase that suppresses defense gene expression.
通讯机构：
[Yan, QY; He, ZL ] S;Sun Yat Sen Univ, Sch Environm Sci & Engn, Southern Marine Sci & Engn Guangdong Lab Zhuhai, State Key Lab Biocontrol,Environm Microbi Res Ctr, Guangzhou 510006, Peoples R China.;Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519080, Peoples R China.
关键词：
Community function;Community stability;Denitrification;Phylogenetic diversity;Species interaction;Synthetic microbial community
摘要：
Denitrification is an important process of the global nitrogen cycle as some of its intermediates are environmentally important or related to global warming. However, how the phylogenetic diversity of denitrifying communities affects their denitrification rates and temporal stability remains unclear. Here we selected denitrifiers based on their phylogenetic distance to construct two groups of synthetic denitrifying communities: one closely related (CR) group with all strains from the genus Shewanella and the other distantly related (DR) group with all constituents from different genera. All synthetic denitrifying communities (SDCs) were experimentally evolved for 200 generations. The results showed that high phylogenetic diversity followed by experimental evolution promoted the function and stability of synthetic denitrifying communities. Specifically, the productivity and denitrification rates were significantly (P < 0.05) higher with Paracocus denitrificans as the dominant species (since the 50th generation) in the DR community than those in the CR community. The DR community also showed significantly (t = 7.119, df = 10, P < 0.001) higher stability through overyielding and asynchrony of species fluctuations, and showed more complementarity than the CR group during the experimental evolution. This study has important implications for applying synthetic communities to remediate environmental problems and mitigate greenhouse gas emissions.
关键词：
Carbon footprints;Grain yield;Greenhouse gas emissions;Nitrogen reduction;Planting density
摘要：
Excessive application of nitrogen fertilization in farmland systems can cause nitrogen wastage, environmental pollution, and increase greenhouse gas (GHG) emissions. Dense planting is one of the efficient strategies for nitrogen fertilizer reduction within rice production. However, there are paying weak attention to the integrative effect of dense planting with less nitrogen (DPLN) on carbon footprint (CF), net ecosystem economic benefit (NEEB) and its components in double-cropping rice systems. Herein, this work aims to elucidate the effect via field experiments in double-cropping rice cultivation region with the treatments set to conventional cultivation (CK), three treatments of DPLN (DR1, 14% nitrogen reduction and 40,000 hills per ha density increase from CK; DR2, 28% nitrogen reduction and 80,000 hills density increase; DR3, 42% nitrogen reduction and 120,000 hills density increase), and one treatment of no nitrogen (N0). Results showed that DPLN significantly reduced average CH(4) emissions by 7.56%-36%, while increasing annual rice yield by 2.16%-12.37% compared to CK. Furthermore, the paddy ecosystem under DPLN served as a carbon sink. Compared with CK, DR3 increased gross primary productivity (GPP) by 16.04% while decreasing direct GHG emissions by 13.1%. The highest NEEB was observed in DR3, which was 25.38% greater than CK and 1.04-fold higher than N0. Therefore, direct GHG emissions and carbon fixation of GPP were key contributors to CF in double-cropping rice systems. Our results verified that optimizing DPLN strategies can effectively increase economic benefits and reduce net GHG emissions. DR3 achieved an optimal synergy between reducing CF and enhancing NEEB in double-cropping rice systems.
通讯机构：
[Huang, H ; Chen, C] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.;Hunan Engn Res Ctr Rice Field Ecol Planting & Bree, Changsha 410128, Peoples R China.
摘要：
The rice-turtle coculture system is the most special rice-fish integrated farming system. In this study, we selected four paddy fields, including a rice monoculture paddy and three rice-turtle paddies with different planting years, to investigate the soil bacterial community composition with Illumina MiSeq sequencing technology. The results indicated that the contents of soil available nitrogen (AN), soil available phosphorus (AP) and soil organic matter (OM) in 9th year of rice-turtle paddy (RT9) were increased by 5.40%, 51.11% and 23.33% compared with rice monoculture paddy (CK), respectively. Significant differences of Acidobacteria, Desulfobacteria, Crenarchaeota were observed among the different rice farming systems. The relative abundance of Methylomonadaceae, Methylococcaceae and Methylophilaceae in RT9 was significantly higher than that in other treatments. RT9 had significantly lower relative abundance of Acidobacteria, but significantly higher relative abundance of Proteobacteria than other treatments. Redundancy analysis showed that soil AN and AP contents were the major factors influencing the abundance of the dominant microbes, wherein Methylomonadaceae, Methylococcaceae and Methylophilaceae were positively correlated with OM. The findings revealed the rice-turtle coculture system in the 9th year had higher soil nutrients and soil bacterial diversity, but there was also a risk of increasing methane emissions.
期刊：
Journal of Environmental Management,2022年317:115374 ISSN：0301-4797
通讯作者：
Huaqin Xu<&wdkj&>Jianwu Tang
作者机构：
[Zhang, Lang; Tang, Jianwu; Ren, Linjing; Hua, Yujie] East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai, Peoples R China.;[Zheng, Huabing; Zhang, Lang; Li, Linlin; Tang, Qiyuan; Wang, Jilong; Xu, Huaqin] Hunan Agr Univ, Coll Agron, Changsha, Hunan, Peoples R China.;[Tang, Jianwu] Minist Educ, Yangtze Delta Estuarine Wetland Ecosyst Observat &, Shanghai, People R China.;[Tang, Jianwu] Shanghai Sci & Technol Comm, Shanghai, Peoples R China.;[Tang, Jianwu] East China Normal Univ, Inst Ecochongming, Shanghai, Peoples R China.
通讯机构：
[Huaqin Xu] C;[Jianwu Tang] S;State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai, PR China<&wdkj&>Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station, Ministry of Education & Shanghai Science and Technology Committee, Shanghai, PR China<&wdkj&>Institute of Eco-Chongming, East China Normal University, Shanghai, PR China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha, Hunan, PR China
摘要：
Increasing both carbon (C) sequestration and food production is essential for a sustainable future. However, increasing soil C sequestration or graining yield/quality in rice (Oryza sativa L.) systems has been a tradeoff in that pursuing one goal may compromise the other goal. Field experiments were designed to evaluate methane emission and grain yield in two rice systems in southern China, including the traditional double rice with a seedling transplanting system and innovative ratoon rice with a direct seeding system. Grain yield, grain quality, methane (CH(4)) emission, and total organic carbon (TOC) loss rate were investigated, and yield-scaled CH(4) gas emission was assessed. It is found that double rice has a higher grain yield than ratoon rice. However, the grain quality (processing, appearance of chalkiness degree and chalky grain percentage, and nutritional quality) of ratoon rice is superior to double rice, especially the ratoon crop. The yield-scaled CH(4) emission of ratoon rice (0.06kgkg(-1)) decreased by 49.29% than double rice (0.12kgkg(-1)) throughout the growth period. Compared with the TOC loss rate of double rice (2.95gkg(-1)), the rate of ratoon rice was lower (1.97gkg(-1)). As a result, ratoon rice with direct seeding can not only improve grain quality but also mitigate yield-scaled CH(4) gas emission and TOC loss rate of rice fields. Therefore, we suggest to use ratoon rice with a direct seeding technique to promote agricultural C sequestration.
摘要：
Fatty acid composition and content affect rapeseed oil quality. Fatty acid synthesis-related genes in rapeseed have been studied globally by researchers. Nevertheless, rapeseed oil is mainly composed of seven different fatty acids (FA), and each fatty acid was regulated by different genes. Furthermore, different FA affect each other, which needs continuous and in-depth research to obtain more clear results in Brassica napus. In this paper, broad-scale miRNA expression profiles were constructed and 21 differentially expressed miRNAs were detected. GO enrichment analysis showed that most up-regulated proteins were involved in transcription factor activity and catalytic activity. KEGG pathway enrichment analysis indicated that 20 pathways involving 36 target genes were enriched, of which the bna00592 pathway may be involved in fatty acid metabolism. The results were verified using a quantitative real-time PCR (RT-qPCR) analysis, we found that the target gene of bna-miR156b > c > g was the OPR (12-oxo-phytodienoic acid reductase). Four copies of OPR gene were found, and the over-expression vectors (pCAMBIA1300-35 s-OPR and pCAMBIA1300-RNAi-OPR) were constructed to verify their functions. In T1 and T2 generation, the content of linoleic acid (LA) increased significantly in OE but deceased in OPRi. This is the first study to provide four copies of the OPR gene that regulates LA metabolism, can be used for the molecular mechanism of LA and optimizing fatty acid profiles in oilseed for breeding programs.
期刊：
Trends in Plant Science,2022年27(8):828-829 ISSN：1360-1385
通讯作者：
Cheng Huang
作者机构：
[Xu, Ying] College of Agronomy, Hunan Agricultural University, Changsha 410128, China;Maize Engineering Technology Research Center of Hunan Province, College of Agronomy, Hunan Agricultural University, Changsha 410128, China;[Wang, Zhili; Li, Man-Wah; Lam, Hon-Ming] Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 999077, China;[Li, Ruilian; Luo, Hongbing] College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Maize Engineering Technology Research Center of Hunan Province, College of Agronomy, Hunan Agricultural University, Changsha 410128, China;[Huang, Cheng] College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Maize Engineering Technology Research Center of Hunan Province, College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 999077, China
通讯机构：
[Cheng Huang] C;College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Maize Engineering Technology Research Center of Hunan Province, College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 999077, China
作者机构：
[Xiao, Fanshu] Guangdong Acad Med Sci, Guangdong Prov Peoples Hosp, Ctr Precis Med, Med Res Ctr, Guangzhou, Peoples R China.;[Yan, Qingyun; Shu, Longfei; Huang, Zhijian; Liu, Shengwei; Li, Juan; He, Zhili; Yu, Huang; Xiao, Fanshu; Wang, Cheng; Chen, Pubo; He, Jianguo] Sun Yat Sen Univ, Sch Environm Sci & Engn, Southern Marine Sci & Engn Guangdong Lab Zhuhai, State Key Lab Biocontrol,Environm Microbi Res Ctr, Guangzhou, Peoples R China.;[Huang, Jie; Yu, Yuhe; Zhu, Wengen] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China.;[Li, Juan; He, Zhili] Hunan Agr Univ, Coll Agron, Changsha, Peoples R China.;[Wang, Jianjun] Chinese Acad Sci, Nanjing Inst Geog & Limnol, Nanjing, Peoples R China.
通讯机构：
[Qingyun Yan ] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou, China
摘要：
Understanding interactions within the gut microbiome and its stability are of critical importance for deciphering ecological issues within the gut ecosystem. Recent studies indicate that long-term instability of gut microbiota is associated with human diseases, and recovery of stability is helpful in the return to health. However, much less is known about such topics in fish, which encompass nearly half of all vertebrate diversity. Here, we examined the assembly and succession of gut microbiota in more than 550 zebrafish, and evaluated the variations of microbial interactions and stability across fish development from larva to adult using molecular ecological network analysis. We found that microbial interactions and stability in the fish gut ecosystem generally increased with host development. This could be attributed to the development of the zebrafish immune system, the increasing amount of space available for microbial colonization within the gut, and the greater stability of nutrients available for the colonized microbiota in adult zebrafish. Moreover, the potential keystone taxa, even those with relatively low abundances, played important roles in affecting the microbial interactions and stability. These findings indicate that regulating rare keystone taxa in adult fish may have great potential in gut microbial management to maintain gut ecosystem stability, which could also provide references for managing gut microbiota in humans and other animals. IMPORTANCE Understanding gut microbial stability and the underlying mechanisms is an important but largely ignored ecological issue in vertebrate fish. Here, using a zebrafish model and network analysis of the gut microbiota we found that microbial interactions and stability in the gut ecosystem increase with fish development. This finding has important implications for microbial management to maintain gut homeostasis and provide better gut ecosystem services for the host. First, future studies should always consider using fish of different age groups to gain a full understanding of gut microbial networks. Second, management of the keystone taxa, even those that are only present at a low abundance, during the adult stage may be a viable pathway to maintain gut ecosystem stability. This study greatly expands our current knowledge regarding gut ecosystem stability in terms of ecological networks affected by fish development, and also highlights potential directions for gut microbial management in humans and other animals.
通讯机构：
[Jia-Shi Peng; Shuan Meng] A;Authors to whom correspondence should be addressed.<&wdkj&>School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Rice Stress Biology, Changsha 410128, China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha 410128, China
关键词：
Cd accumulation;Cd tolerance;S. plumbizincicola;ZIP2
摘要：
Zinc (Zn)-regulated and iron (Fe)-regulated transporter-like proteins (ZIP) are key players involved in the accumulation of cadmium (Cd) and Zn in plants. Sedum plumbizincicola X.H. Guo et S.B. Zhou ex L.H. Wu (S. plumbizincicola) is a Crassulaceae Cd/Zn hyperaccumulator found in China, but the role of ZIPs in S. plumbizincicola remains largely unexplored. Here, we identified 12 members of ZIP family genes by transcriptome analysis in S. plumbizincicola and cloned the SpZIP2 gene with functional analysis. The expression of SpZIP2 in roots was higher than that in the shoots, and Cd stress significantly decreased its expression in the roots but increased its expression in leaves. Protein sequence characteristics and structural analysis showed that the content of alanine and leucine residues in the SpZIP2 sequence was higher than other residues, and several serine, threonine and tyrosine sites can be phosphorylated. Transmembrane domain analysis showed that SpZIP2 has the classic eight transmembrane regions. The evolutionary analysis found that SpZIP2 is closely related to OsZIP2, followed by AtZIP11, OsZIP1 and AtZIP2. Sequence alignment showed that most of the conserved sequences among these members were located in the transmembrane regions. A further metal sensitivity assay using yeast mutant Δyap1 showed that the expression of SpZIP2 increased the sensitivity of the transformants to Cd but failed to change the resistance to Zn. The subsequent ion content determination showed that the expression of SpZIP2 increased the accumulation of Cd in yeast. Subcellular localization showed that SpZIP2 was localized to membrane systems, including the plasma membrane and endoplasmic reticulum. The above results indicate that ZIP member SpZIP2 participates in the uptake and accumulation of Cd into cells and might contribute to Cd hyperaccumulation in S. plumbizincicola.
通讯机构：
[Qingyun Yan; Zhili He] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, China<&wdkj&>Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha, China
关键词：
Phosphorus cycling gene/microorganism;Database;Accuracy;Comprehensiveness;Metagenome sequencing data
摘要：
Phosphorus (P) is one of the most essential macronutrients on the planet, and microorganisms (including bacteria and archaea) play a key role in P cycling in all living things and ecosystems. However, our comprehensive understanding of key P cycling genes (PCGs) and microorganisms (PCMs) as well as their ecological functions remains elusive even with the rapid advancement of metagenome sequencing technologies. One of major challenges is a lack of a comprehensive and accurately annotated P cycling functional gene database. In this study, we constructed a well-curated P cycling database (PCycDB) covering 139 gene families and 10 P metabolic processes, including several previously ignored PCGs such as pafA encoding phosphate-insensitive phosphatase, ptxABCD (phosphite-related genes), and novel aepXVWPS genes for 2-aminoethylphosphonate transporters. We achieved an annotation accuracy, positive predictive value (PPV), sensitivity, specificity, and negative predictive value (NPV) of 99.8%, 96.1%, 99.9%, 99.8%, and 99.9%, respectively, for simulated gene datasets. Compared to other orthology databases, PCycDB is more accurate, more comprehensive, and faster to profile the PCGs. We used PCycDB to analyze P cycling microbial communities from representative natural and engineered environments and showed that PCycDB could apply to different environments. We demonstrate that PCycDB is a powerful tool for advancing our understanding of microbially driven P cycling in the environment with high coverage, high accuracy, and rapid analysis of metagenome sequencing data. The PCycDB is available at https://github.com/ZengJiaxiong/Phosphorus-cycling-database. Video Abstract
通讯作者：
Xuemei Chen<&wdkj&>Nenghui Ye<&wdkj&>Jianhua Zhang<&wdkj&>Guanqun Wang
作者机构：
[Wang, Guanqun; Li, Haoxuan; Zhang, Jianhua] Hong Kong Baptist Univ, Dept Biol, Hong Kong, Peoples R China.;[Wang, Guanqun; Li, Haoxuan; Zhang, Jianhua] Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Peoples R China.;[Wang, Guanqun; Li, Haoxuan; Zhang, Jianhua] Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Peoples R China.;[Li, Haoxuan; Ye, Nenghui] Hunan Agr Univ, Coll Agr, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.;[Chen, Xuemei; You, Chenjiang] Univ Calif Riverside, Dept Bot & Plant Sci, Inst Integrat Genome Biol, Riverside, CA 92521 USA.
通讯机构：
[Xuemei Chen; Jianhua Zhang; Guanqun Wang] D;[Nenghui Ye] S;Department of Biology, Hong Kong Baptist University, Hong Kong, China<&wdkj&>School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China<&wdkj&>Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, USA<&wdkj&>Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Agriculture, Hunan Agricultural University, Changsha, China
摘要：
Male sterility enables hybrid crop breeding to increase yields and has been extensively studied. But thermo-sensitive female sterility, which is an ideal property that may enable full mechanization in hybrid rice breeding, has rarely been investigated due to the absence of such germplasm. Here we identify the spontaneous thermo-sensitive female sterility 1 (tfs1) mutation that confers complete sterility under regular/high temperature and partial fertility under low temperature as a point mutation in ARGONAUTE7 (AGO7). AGO7 associates with miR390 to form an RNA-Induced Silencing Complex (RISC), which triggers the biogenesis of small interfering RNAs (siRNAs) from TRANS-ACTING3 (TAS3) loci by recruiting SUPPRESSOR OF GENE SILENCING (SGS3) and RNA-DEPENDENT RNA POLYMERASE6 (RDR6) to TAS3 transcripts. These siRNAs are known as tasiR-ARFs as they act in trans to repress auxin response factor genes. The mutant TFS1 (mTFS1) protein is compromised in its ability to load the miR390/miR390* duplex and eject miR390* during RISC formation. Furthermore, tasiR-ARF levels are reduced in tfs1 due to the deficiency in RDR6 but not SGS3 recruitment by mTFS1 RISC under regular/high temperature, while low temperature partially restores mTFS1 function in RDR6 recruitment and tasiR-ARF biogenesis. A miR390 mutant also exhibits female sterility, suggesting that female fertility is controlled by the miR390-AGO7 module. Notably, the tfs1 allele introduced into various elite rice cultivars endows thermo-sensitive female sterility. Moreover, field trials confirm the utility of tfs1 as a restorer line in fully mechanized hybrid rice breeding.
通讯机构：
[Wenbang Tang; Xuedan Lu] C;College of Agronomy, Hunan Agricultural University, Changsha 410128, PR China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha 410128, PR China<&wdkj&>State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, 410125, PR China
关键词：
Energy saving;Factory nursery;Pc-LEDs;Tobacco
摘要：
Industrial Seedling Raising (ISR) is increasingly becoming an important part of Modern Agriculture because of its efficient utilization of land, water, and fertilizer as well as its advantages of being not easily affected by the weather. However, the high cost and high energy consumption of light sources for plant growth is limiting the popularization of ISR technology. Phosphor-converted light-emitting diodes (pc-LEDs) make use of relatively affordable red phosphor and blue light chips, providing an adjustable spectrum to optimize plant growth. To identify the energy-saving light quality of pc-LEDs, we investigated the effects of a variety of light qualities on the growth of tobacco seedlings. Y3Al5O12:Ce3+, CaAlSiN3:Eu2+, KAl11O17:Eu2+ phosphors were combined with the blue light chip according to different proportions to produce the following light sources: CK (white light), T1 (blue light), T2 (red light), T3 (red: blue light ratio = 1:4), T4 (red: blue light ratio = 4:1). The tobacco variety Xiangyan7 grown continuously under T1, T2, T3, and T4 significantly increased the leaf area, stem length, biomass, root area and main root length compared with those grown under white light. Among the five kinds of light qualities tested, T4 treatment exerted the best effect on leaf development and biomass increase, while T2 exerted the best effect on stem elongation. The cytological analysis demonstrated that the promotion of the cell size and cell number of leaf epidermal cells by T1-T4 might contribute to the leaf expansion. Further analysis at the molecular level suggested that the light quality affected the RNA levels of the genes involved in cell division and expansion. When tobacco seedlings reached the same biomass, T1-T4 light sources saved 71%, 86%, 80% and 89% of electric energy respectively compared with white light. Therefore, the application of specific pc-LEDs not only reduces the cost of light source production, but also saves energy consumption, offering great potential for ISR technology to cut costs and increase efficiency.
作者机构：
[Deng, HF; Guo Xia-Yu; Deng Hua-Feng] Hunan Agr Univ, Coll Agron, Changsha 410125, Peoples R China.;[Guo Xia-Yu; Ai Zhi-Yong; Ai, ZY] Natl Innovat Ctr Saline Alkali Tolerant Rice Sany, Sanya 572000, Peoples R China.;[Li Jian-Wu; Guo Xia-Yu; Long Ji-Rui; Ai Zhi-Yong; Ai, ZY; Wei Zhong-Wei] Hunan Hybrid Rice Res Ctr, Changsha 410125, Peoples R China.;[Zhang Meng] Hunan Univ, Coll Biol, Hunan Key Lab Plant Funct Genom & Dev Regulat, Changsha 410082, Hunan, Peoples R China.;[Zhou Bin; Zhu Ming-Dong] Hunan Rice Res Inst, Key Lab Ind Rice Genet & Breeding Middle & Lower, Changsha 410125, Peoples R China.
通讯机构：
[Deng, HF ; Zhou, B ] H;[Ai, ZY ] N;Hunan Agr Univ, Coll Agron, Changsha 410125, Peoples R China.;Natl Innovat Ctr Saline Alkali Tolerant Rice Sany, Sanya 572000, Peoples R China.;Hunan Hybrid Rice Res Ctr, Changsha 410125, Peoples R China.
关键词：
Super hybrid rice;CY1000;Salt tolerance;Molecular mechanism;Ubiquitination
摘要：
Soil salinization is a threat to food security. China is rich in saline land resources for potential and current utilization. The cultivation and promotion of salt-tolerant rice varieties can greatly improve the utilization of this saline land. The super hybrid rice Chaoyouqianhao (CY1000) is one of the most salt-tolerant rice varieties and is widely used, but the molecular mechanism underlying its salt tolerance is not clear. In this study, the characteristics of CY1000 and its parents were evaluated in the field and laboratory. The results showed that aboveground parts of CY1000 were barely influenced by salt stress, while the roots were less affected than those of its parents. A comparative transcriptomic strategy was used to analyze the differences in the response to salt stress among the male and female parents of CY1000 at the seedling stage and the model indica rice 93–11. We found that the salt tolerance of CY1000 was mainly inherited from its male parent R900, and its female parent GX24S showed hardly any salt tolerance. To adapt to salt stress, CY1000 and R900 upregulated the expression of genes associated with soluble component synthesis and cell wall synthesis and other related genes and downregulated the expression of most genes related to growth material acquisition and consumption. In CY1000 and R900, the expression of genes encoding some novel key proteins in the ubiquitination pathway was significantly upregulated. After treatment with MG-132, the salt tolerance of CY1000 and R900 was significantly decreased and was almost the same as that of the wild type after salt stress treatment, indicating that ubiquitination played an important role in the salt tolerance mechanism of CY1000. At the same time, we found that some transcription factors were also involved in the salt stress response, with some transcription factors responding only in hybrid CY1000, suggesting that salt tolerance heterosis might be regulated by transcription factors in rice. Our results revealed that the ubiquitination pathway is important for salt tolerance in rice, and several novel candidate genes were identified to reveal a novel salt tolerance regulation network. Additionally, our work will help clarify the mechanism of heterosis in rice. Further exploration of the molecular mechanism underlying the salt tolerance of CY1000 can provide a theoretical basis for breeding new salt-tolerant rice varieties.
