期刊：
Journal of Hazardous Materials,2021年411:125094 ISSN：0304-3894
通讯作者：
Qingyun Yan
作者机构：
[Yan, Xizhe; Yan, Qingyun; Weng, Wanlin; Peng, Tao; Shu, Longfei; Xu, Kui; He, Zhili; Yu, Huang; Zheng, Xiafei; Xiao, Fanshu; Liu, Xingyu; Wang, Cheng; Chen, Pubo; Zhong, Qiuping] Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Sch Environm Sci & Engn, Environm Microbi Res Ctr, Guangzhou 510006, Peoples R China.;[Yang, Tony] Agr & Agri Food Canada, Swift Current Res & Dev Ctr, Swift Current, SK S9H 3X2, Canada.;[He, Zhili] Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
通讯机构：
[Qingyun Yan] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou 510006, China
摘要：
The impacts of metal(loids) on soil microbial communities are research focuses to understand nutrient cycling in heavy metal-contaminated environments. However, how antimony (Sb) and arsenic (As) contaminations synergistically affect microbially-driven ecological processes in the rhizosphere of plants is poorly understood. Here we examined the synergistic effects of Sb and As contaminations on bacterial, archaeal and fungal communities in the rhizosphere of a pioneer plant (Miscanthus sinensis) by focusing on soil carbon and nitrogen cycle. High contamination (HC) soils showed significantly lower levels of soil enzymatic activities, carbon mineralization and nitrification potential than low contamination (LC) environments. Multivariate analysis indicated that Sb and As fractions, pH and available phosphorus (AP) were the main factors affecting the structure and assembly of microbial communities, while Sb and As contaminations reduced the microbial alpha-diversity and interspecific interactions. Random forest analysis showed that microbial keystone taxa provided better predictions for soil carbon mineralization and nitrification under Sb and As contaminations. Partial least squares path modeling indicated that Sb and As contaminations could reduce the carbon mineralization and nitrification by influencing the microbial biomass, alpha-diversity and soil enzyme activities. This study enhances our understanding of microbial carbon and nitrogen cycling affected by Sb and As contaminations.
通讯机构：
[Qingyun Yan] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou 510006, China
摘要：
Small ponds have become a hotspot of greenhouse gas emissions, but our understanding of methane (CH4) cycling and its biological regulation in small polluted ponds remains limited. To assess how pollution affects CH4 content, we investigated dissolved CH4 concentrations, water and sediments properties, methanogenic and methanotrophic communities in two types of small polluted ponds. Compared with low pollution (LP) ponds, high pollution (HP) ponds showed significantly (P < 0.05) higher dissolved CH4 in water. Sequencing of methyl coenzyme M reductase (mcrA) and particulate methane monooxygenase (pmoA) genes showed that HP led to significant (P < 0.05) shifts of CH4-cycling microbial communities, with increased Shannon index of sediment methanogenic communities and water methanotrophic communities. There were also strong negative associations (P < 0.05) between dissolved CH4 concentrations and interdomain methanogen-methanotroph network connectivity in water and sediments, respectively. The partial least squares path modeling indicated that dissolved oxygen, total organic carbon, ammonium nitrogen and nitrate nitrogen of water, and total nitrogen and total carbon of sediment, and CH4-cycling microbes could regulate the CH4 content. This study clarified the effects of environmental deterioration on CH4 cycling in small ponds, highlighting the use of methanogen-methanotroph network connectivity to assess the CH4 production. (C) 2021 Elsevier B.V. All rights reserved.
通讯机构：
[Fanshu Xiao; Qingyun Yan] E;Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Guangzhou, 510006, China
期刊：
Science of The Total Environment,2021年791:147890 ISSN：0048-9697
通讯作者：
Cao Cougui
作者机构：
[Ling Lin; Maimaitizunong, Ayitula] Huazhong Agr Univ, Coll Plant Sci & Technol, Macro Res Agr Inst, Wuhan 430070, Peoples R China.;[Wang Jinping; Shuai Yanju; Li Chengfang; Ling Lin; Zhan Ming; Cao Cougui; Zhang Haoran; Jiang Yang; Maimaitizunong, Ayitula; Sun Zichuan] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China.;[Xu Ying] Hunan Agr Univ, Coll Agr, Changsha 410128, Peoples R China.;[Zhang Zhisheng] Hubei Acad Agr Sci, Hubei Key Lab Food Crop Germplasm & Genet Improve, Food Crops Inst, Wuhan 430064, Peoples R China.;[Wang Bin] Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China.
通讯机构：
[Cao Cougui] C;College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
关键词：
Carbon footprint;Economic benefit;GHG mitigation practices;Integrated farming system;Rice production system;Rotation
摘要：
In recent years, many rotational and integrated rice production systems coupled with several greenhouse gas (GHG) emissions mitigation practices have been developed and adopted for demand of low carbon production. However, there have been only few studies about comparisons on the balance between high production and mit-igation of GHG emissions in different rice production systems. We therefore aimed to evaluate economic output and carbon footprint of different rice production systems, based on several long-term experiments conducted by our lab. CH4 and N2O emission were measured by the same static chamber/gas chromatogram measurement pro-cedure in different rice production systems, including rice-fallow, rice-rapeseed, rice-wheat, double rice, and in-tegrated rice-crayfish production system. Then, we applied the DeNitrification DeComposition model to simulate CH4 and N2O emission over different years under the same condition for comparison. Carbon footprint was cal-culated following the process-based life cycle assessment (PLCA) methodology. The economic benefit of rice pro-duction systems was assessed by cost-benefit analysis. According to the analysis, the double-rice production system exhibited the highest intensity of carbon footprint (ICF = 4.14 kg CO2-eq yuan(-1)), rain-fed treatment in the rice-rapeseed system had the lowest (ICF = 0.68 kg CO2-eq yuan(-1)). The intensity of carbon footprint in different treatments in the integrated rice-crayfish production system was around 0.8 kg CO2-eq yuan(-1). Over-all, the results of this case study suggest: (1) the proposed practices in different rice production systems are no straw returning (rice-fallow), no-tillage without straw returning (rice-wheat), rain-fed farming (rice-rapeseed), no insect and no inoculation (double rice), and feeding with straw returning (rice-crayfish); (2) rotational and integrated systems can achieve high net output with low carbon emission; (3) reducing the amount of nitrogenous fertilizer application is the most important and effective GHG mitigation practice for rotational systems. (C) 2021 Published by Elsevier B.V.
作者机构：
[丛韫起; 郑铖; 闫凌月; 段美娟; 张豪健; 樊帆; 郑雨晴; 刘次桃] Hunan Provincial Key Laboratory of Rice Stress Biology, College of Agriculture, Hunan Agricultural University, Changsha 410128, China;[袁定阳; 袁贵龙] State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China;[潘根] Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
通讯机构：
[Yanhong Lou; Yuping Zhuge] N;National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, Shandong Agricultural University, Tai’an City, Shandong 271018, China
摘要：
With the increasing production and wide application of carbon nanotubes (CNTs), they are inevitably released into the natural environment and ecosystems, where plants are the main primary producers. Hence, it is imperative to understand the toxic effects of CNTs on plants. The molecular mechanisms underlying the toxic effects of CNTs on plants are still unclear. Therefore, in the present study, we investigated the effects of high concentrations of multi-walled CNTs (MWCNTs) on Arabidopsis. Root elongation and leaf development were severely inhibited after MWCNT exposure. Excess production of H2O2, O-2(-), and malondialdehyde was observed, indicating that MWCNTs induced oxidative stress. The antioxidant system was activated to counter MWCNTs-induced oxidative stress. Combinatorial transcriptome and m6A methylome analysis revealed that MWCNTs suppressed auxin signaling and photosynthesis. Reactive oxygen species metabolism, toxin metabolism, and plant responses to pathogens were enhanced to cope with the phytotoxicity of MWCNTs. Our results provide new insights into the molecular mechanisms of CNT phytotoxicity and plant defense responses to CNTs. (C) 2021 Elsevier B.V. All rights reserved.
作者机构：
[Luo, Qiuhong; Lei, Dongyang; Yao, Dongping] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;[Wu, Jun; Deng, Qiyun; Zhuang, Wen; Xiao, Gui; Bai, Bin; Yao, Dongping; Li, Jianwu] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.;[Deng, Qiyun] Biorice Hunan Co Ltd, Changsha 410323, Hunan, Peoples R China.
通讯机构：
[Lei, Dongyang; Bai, Bin] H;Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.;Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.
关键词：
Grain filling stage;High natural field temperature;Morphological structure;Physicochemical properties;Rice starch
摘要：
This study investigated the effects of natural high temperature in the field during grain filling stage on the morphological structure and physicochemical properties of rice starch. High natural field temperature during rice grain filling stage resulted in poor processing and appearance quality, higher gelatinization properties including gelatinization temperature, gelatinization enthalpy, swelling power, and water solubility due to the reduction of amylose content. High temperature decreased the setback and trough viscosities, and increased breakdown, implying that the pasting properties were slightly better. High temperature did not change the starch crystalline type, while it significantly affected relative crystallinity, as well as pitting and unevenness on the surface of the starch granules with lower granule size. The above results imply that high temperature can degrade cooking and eating quality, and increase pasting properties of starch slightly.
摘要：
Winter fallow is important for renewing and improving soil fertility under double-cropping rice systems, such as those in southern China. Using a regenerative farming technology of integrating grass-chicken farming in a winter fallow field, we investigated soil nitrogen conversion and assessed the agricultural economic benefits of the whole farmland ecosystem. To test the effects of chicken grazing on the fallow system, we established field treatments involving adding chickens to a field planted with the cover crops, including cover milk vetch (Astragalus sinicus) with chicken grazing treatment (MC) and cover ryegrass (Lolium spp.) with chicken grazing (RC); cover crops only, including cover milk vetch (Astragalus sinicus) treatment (M) and cover ryegrass (Lolium spp.) (R); and a bare fallow field treatment (CK). We found that both cover crops (M and R) and cover crops with chicken grazing (MC and RC) increased nitrate, ammonium, dissolved organic nitrogen, and total nitrogen contents, and the increase was higher in MC and RC treatments. We also observed increased straw biomass and grain yield in the all four treatments, with more increases with chicken treatments as compared with CK. On the economic profits, MC increased by 101.72% and RC increased by 104.12% as compared with CK, while R increased by 5.19% and M reduced by 1.86% as compared with CK. The nitrogen transfer rate (the output/input ratio) of MC, RC, M, and R increased by 66.71%, 71.50%, 65.97%, and 59.97%, respectively, while the nitrogen accumulation rate (input-output) of MC, RC, M, and R increased by 480.56%, 612.98%, 356.74%, and 267.65%, respectively. Our study demonstrates that retaining nitrogen and gaining economic profit by integrating cover crops with chicken grazing is potentially more sustainable than adding cover crops alone. We further suggest that using the integrated grass-livestock farming technology can reduce environmental damage caused by commercial fertilizers. (C) 2019 Elsevier B.V. All rights reserved.
关键词：
Cd distribution;Cd-safe tubers;Phytoremediation;Potato (Solanum tuberosum L.)
摘要：
Cadmium (Cd) is a toxic element that can accumulate in plants and poses a threat to human health through biomagnification. There are differences in Cd levels among different plants tissues. Hence, an optimal crop that possesses low Cd levels in the edible parts but high levels in the inedible parts is urgently needed to simultaneously lower soil-Cd levels in contaminated fields and to produce Cd-safe crops. In this study, we investigated the Cd levels in tubers and other tissues of potato (Solanum tuberosum L.) using different experimental approaches, and identified variations in Cd accumulation in different potato cultivars and characterized the Cd-distribution pattern in potato. Our results showed that Cd accumulation in tubers of the tested cultivars varied greatly, and that tuber-Cd levels were much lower than in the stems or leaves. Two-way ANOVA revealed that the tuber-Cd levels in potato are determined by both genotypic differences and the soil-Cd levels of the farmlands. Among the cultivars tested, one cultivar, 'Eshu10', was found to have the lowest tuber-Cd levels but had much higher Cd levels in leaf and stem tissues. Our study shows that the Cd-distribution pattern within potato plants makes it an ideal candidate for the safe production of a staple food that also has the potential to contribute to the remediation of contaminated soils. (C) 2019 Elsevier B.V. All rights reserved.
摘要：
Anaerobic digestion is widely used in starch wastewater pre-treatment and can remove the COD effectively, however, the effluents are nutritious and often need supplemental aerobic treatments to remove nutrients prior to discharge. The objective of this study was to investigate the feasibility of using the liquid digestate of starch wastewater (LDSW) as a fertilizer. This pot experiment was conducted with Ipomoea aquatica Forsk in a greenhouse with six treatment groups. The crop growth was significantly promoted, while the accumulation of soil nitrate was not influenced after LDSW addition, compared to the control. In addition, at the same nitrogen input, the yield of high-LDSW treatment was 65.2%, 92.3% and 69.2% higher than those of chemical fertilizer treatment during the three growth periods. Furthermore, average N2O emission with high-LDSW addition was 15.8 g N/(ha.d), accounting for 15.0% of which under high chemical fertilizer treatment, due to the significantly enhanced denitrification genes (nirK, nirS and nosZ) abundance. Besides, the changes of soil N2O-reducing bacteria were performed by high-throughput sequencing of nosZ. Our findings suggested that LDSW had many opportunities for sustainable agriculture to guarantee high yields while reducing negative environmental impacts. (C) 2020 Elsevier B.V. All rights reserved.
摘要：
There have been no studies demonstrating the correlation between soil nutrient heterogeneity and cadmium (Cd) absorption of Bermudagrass. In this study, a pot experiment was carried out to study the correlation between them. The purpose is to find soil nutrient factors which are conducive to improving the Cd absorption and translocation. The eighth group had the largest total number of surviving plants, the highest Fv/Fo value (3.24) and the best growth characteristics. The fifth group had the lowest total number of surviving plants, Fv/Fo (2.47) and the worst growth. The Cd content of the fifth group (36.11 mg kg(-1)) was close to the eighth group (35.72 mg kg-1), but the two groups had significant differences in plant height, stem node length and stem node number (P &lt; 0.05). The eighth group showed the highest contents of nitrate nitrogen (NO3--N), available potassium and urease activity. The fifth group showed the lowest NO3--N content, but the highest ammonium nitrogen (NH4+-N) and available phosphorus content. There was significant difference of the Cd bioconcentration factors (BCF) and trans location factor (TCF) between the fifth and the eighth group although they had the similar total soil Cd content (P &lt; 0.05). The fifth group had the highest BCF and TCF. RDA analysis indicated the BCF and TCF were positively correlated with soil NH4+-N and available phosphorus and negatively correlated with soil NO3--N. The results demonstrated that soil NHI-N and available phosphorus were important soil ecological factors to enhance Cd absorption and translocation of bermudagrass. (C) 2019 Elsevier Ltd. All rights reserved.
期刊：
International Journal of Molecular Sciences,2019年20(6):ARTN 1427 ISSN：1661-6596
通讯作者：
Zhang, Jian;Ao, Hejun
作者机构：
[Liu, Xixi; Li, Zhiyong; Hou, Yuxuan; Wang, Yifeng; Wang, Huimei; Tong, Xiaohong; Zhang, Jian] China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 311400, Zhejiang, Peoples R China;[Ao, Hejun] Hunan Agr Univ, Coll Agr Sci, Changsha 410128, Hunan, Peoples R China
通讯机构：
[Zhang, Jian] C;[Ao, Hejun] H;China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 311400, Zhejiang, Peoples R China. Hunan Agr Univ, Coll Agr Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
ABA;SAPK;SnRK2s;bZIP;flowering time;rice (Oryza sativa L.)
摘要：
As core components of ABA signaling pathway, SnRK2s (Sucrose nonfermenting1(-)Related protein Kinase 2) bind to and phosphorylate AREB/ABF (ABA responsive element binding protein/ABRE-binding factor) transcriptional factors, particularly bZIPs (basic region-leucine zipper), to participate in various biological processes, including flowering. Rice contains 10 SnRK2 members denoted as SAPK1-10 (Stress-Activated Protein Kinase) and dozens of bZIPs. However, which of the SAPKs and bZIPs pair and involve in ABA signaling remains largely unknown. In this study, we carried out a systematical protein-protein interactomic analysis of 10 SAPKs and 9 ABA-inducible bZIPs using yeast-two-hybrid technique, and identified 14 positive interactions. The reliability of Y2H work was verified by in vitro pull-down assay of the key flowering regulator bZIP77 with SAPK9 and SAPK10, respectively. Moreover, SAPK10 could phosphorylate bZIP77 in vitro. Over-expression of SAPK10 resulted in earlier flowering time, at least partially through regulating the FAC-MADS15 pathway. Conclusively, our results provided an overall view of the SAPK-bZIP interactions, and shed novel lights on the mechanisms of ABA-regulated rice flowering.
摘要：
The ecological implications of livestock production intensification have received sustained attention across the globe. Anaerobic digestion is the main process for livestock waste treatment. However, the ecological consequences of dissolved organic matter originating from anaerobic digestion (AD-DOM) in eutrophic water bodies remain elusive. In this study, the physiological responses of a bloom-forming cyanobacterium, Microcystis aeruginosa, to AD-DOM were investigated. Moreover, the composition of AD-DOM was identified by using thermochemolysis followed by gas chromatography-mass spectrometry (GC-MS) analysis. The growth of M. aeruginosa FACHB905 was not sensitive to low levels (0.625-1.25%, V/V) of AD-DOM but was inhibited by high levels (2.5-5%, V/V) of AD-DOM, resulting from photoinhibition damage to photosystem II (PSII). The main target of AD-DOM in PSII was the electron accepting side (psi(0)) or the electron donor side (phi P-0), depending on time variables. The reactive oxygen species (ROS) level showed a positive correlation with AD-DOM addition; however, it was higher than that of the control for 3.75-5% AD-DOM on the 6th day. The intracellular microcystin contents (including MC-LR and Dha(7)-MC-LR) decreased in response to AD-DOM addition, but extracellular microcystin increased after 6 days of exposure. In addition, GC-MS detection showed that AD-DOM is mainly composed of lignin-derived aromatic compounds, alkanes/alkene, nitrogencontaining compounds, and sterols. The results presented in this study suggested that AD-DOM released from the livestock industry may play a subtle role in affecting harmful algal blooms through level-dependent variables. In addition, the ecological consequences of microcystin released by toxin-producing species under AD-DOM stress are still worth considering. (C) 2019 Elsevier B.V. All rights reserved.
通讯机构：
[Yin, Huaqun] C;[Yin, Huaqun] M;Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;Minist Educ, Key Lab Biomet, Changsha 410083, Peoples R China.
关键词：
Cd solubility;Redox potential;Microbial community;Molecular ecology networks (MENs);Anaerolineaceae
摘要：
Understanding the role of microbes in the solubility of cadmium (Cd) is of fundamental importance for remediation of Cd toxicity. The present study aimed to identify the microbes that involved in regulating Cd solubility and to reveal possible mechanisms. Therefore, microbial communities were investigated through high-throughput sequencing approach, the molecular ecological network was constructed and metagenomes were predicted. Our results indicated that redox conditions affected both the solubility of soil Cd and the microbial communities. Anaerobic microbes, such as Anaerolineaceae, did not only play important roles in shaping the microbial community in soils, but might also be involved in regulating the Cd solubility. Two possible mechanisms that how Anaerolineaceae involved in Cd solubility are (1) Anaerolineaceae are important organic matter degraders under anoxic conditions and (2) Anaerolineaceae can co-exist with methane metabolism microbes, while methane metabolism promotes the precipitation of soluble Cd. Thus, application of Anaerolineaceae in bioremediation of soil Cadmium contamination is a potential approach. The study provided a novel insight into the role of microbial community in the regulation of Cd solubility under different redox conditions, and suggested a potential approach for the remediation of soil Cd contamination. (c) 2018 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
作者机构：
[傅志强; 龙攀; 苏姗; 刘莉; 肖志祥; 李超; 祝志娟; 黄亚男] College of Agronomy, Hunan Agricultural University, Changsha 410128, China
关键词：
双季稻;冬季;种植模式;土壤有机碳;活性有机碳;碳库管理指数
摘要：
为探讨冬季覆盖作物还田对稻田土壤碳库的影响,通过冬季种植油菜、紫云英、黑麦草、马铃薯,并以冬闲为对照进行大田试验,测定了不同冬季作物模式下早稻和晚稻的土壤有机碳、活性有机碳含量,并计算了稳态碳、碳库活度、活度指数、碳库指数和土壤碳库管理指数.结果表明:冬季作物还田增加了土壤有机碳含量,早稻和晚稻后的土壤有机碳含量比对照分别提高了1%～8%和3%～18%;油菜、黑麦草和紫云英还田均促进了土壤活性有机碳含量的增加,早稻后增加16.2%～84.2%,晚稻后增加24.4%～28.1%;冬季作物还田增加了土壤碳库管理指数,增加幅度为1.4%～41.8%.综上所述,冬种作物还田有利于提高土壤的固碳效应,并提升土壤质量,以种植黑麦草、紫云英的综合效果较佳.To explore the effects of winter crops recycling into paddy field on soil carbon pool, we cultivated rape, Chinese milk vetch, ryegrass and potato in winter season after harvesting double-season rice, with fallow as control (CK). The contents of soil organic carbon (SOC) and labile organic carbon (LOC) in the harvest of early and late rice were measured, and the stable carbon, activity of carbon pool, carbon pool index, carbon pool activity index and carbon pool management index (CMI) were calculated. Results showed that winter cover crops returning increased SOC content by 1%-8% and 3%-18% after harvest of early rice and late rice, respectively in comparison with CK. Cultivating rape, Chinese milk vetch and ryegrass all increased the LOC content, with enhancement of 16.2%-84.2% and 24.4%-28.1% after harvest of early rice and late rice, respectively. The CMI of all winter crop treatments presented a growth ratio of 1.4%-41.8% compared to CK. In conclusion, the cultivation of winter crop and recycling to field promoted soil carbon fixation and soil quality, among which the ryegrass and Chinese milk vetch showed better comprehensive effect.
