通讯机构：
[Xiaogang Li] C;College of Plant Protection, Hunan Agricultural University, Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Changsha 410128, China
关键词：
Community structure;Methane;Network;Thifluzamide
摘要：
Thifluzamide is an effective agent for controlling rice sheath blight and has a long half-life in soil. However, the effects of thifluzamide on the abundance of microbes harboring methane-cycle genes and soil microbial community assembly patterns are not well known. Thus, we conducted a three-month indoor mesocosm experiment to ascertain the effects of thifluzamide (0.05, 0.5, and 5 mg kg(-1) soil; 0.05 mg kg(-1) soil being recommended) on bacterial and archaeal community structure and on the abundance of methanogen genes using two typical paddy soils: sandy soil from Hangzhou (HZ) and loam sandy soil from Jiansanjiang (JSJ). The effects of thifluzamide on soil microorganisms were related to soil type. In JSJ loam sandy soil, thifluzamide significantly increased bacterial α diversity after 7-30 d and archaeal α diversity at 30 and 60 d. In HZ sandy soil, however, α diversity did not change significantly. Network analysis showed that thifluzamide-treated soils possessed more complex networks with more total nodes and links, a higher average degree of connectivity, and more keystone species. Thifluzamide application increased the number of keystone species associated with methane production in both types of paddy soil. A relatively greater number of modules were significantly negatively correlated with mcrA abundance in the HZ T10 network, but more modules were positively correlated with mcrA abundance in the JSJ T100 network. The half-life of thifluzamide varied for the different doses, i.e., from 152.0 to 419.6 d. The results reveal that methane-cycle genes, soil microbiome assembly, and interactions among microbial species all change in response to thifluzamide stress.
通讯机构：
[Hong Yue; Yuting Ma; Yubi Lin] T;The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China<&wdkj&>The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China<&wdkj&>College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China<&wdkj&>The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, China<&wdkj&>Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangdong Geriatrics Institute, Guangdong Cardiovascular Institute, Guangzhou, China
摘要：
BACKGROUND: Brugada syndrome (Brs) and long QT syndrome (LQTs) are the most observed "inherited primary arrhythmia syndromes" and "channelopathies", which lead to sudden cardiac death. METHODS: Detailed clinical information of Brs and LQTs patients was collected. Genomic DNA samples of peripheral blood were conducted for whole-exome sequencing on the Illumina HiSeq 2000 platform. Then, we performed bioinformatics analysis for 200 genes susceptible to arrhythmias and cardiomyopathies. Protein interaction and transcriptomic co-expression were analyzed using the online website and GTEx database. RESULTS: All sixteen cases of Brs and six cases of LQTs were enrolled in the current study. Four Brs carried known pathogenic or likely pathogenic of single-point mutations, including SCN5A p.R661W, SCN5A p.R965C, and KCNH2 p.R692Q. One Brs carried the heterozygous compound mutations of DSG2 p.F531C and SCN5A p.A1374S. Two Brs carried the novel heterozygous truncated mutations (MAF < 0.001) of NEBL (p.R882X) and NPPA (p.R107X), respectively. Except for the indirect interaction between NEBL and SCN5A, NPPA directly interacts with SCN5A. These gene expressions had a specific and significant positive correlation in myocardial tissue, with high degrees of co-expression and synergy. Two Brs carried MYH7 p.E1902Q and MYH6 p.R1820Q, which were predicted as "damaging/possibly damaging" and "damaging/damaging" by Polyphen and SIFT algorithm. Two LQTs elicited the pathogenic single splicing mutation of KCNQ1 (c.922-1G > C). Three LQTs carried a single pathogenic mutation of SCN5A p.R1880H, KCNH2 p.D161N, and KCNQ1 p.R243S, respectively. One patient of LQTs carried a frameshift mutation of KCNH2 p. A188Gfs*143. CONCLUSIONS: The truncated mutations of NEBL (p.R882X) and NPPA (p.R107X) may induce Brugada syndrome by abnormally affecting cardiac sodium channel. SCN5A (p.R661W, p.R965C and p.A1374S) and KCNH2 (p.R692Q) may cause Brugada syndrome, while SCN5A (p.R1880H), KCNQ1 (c.922-1G > C and p.R243S) and KCNH2 (p.D161N and p.A188Gfs*143) may lead to long QT syndrome.
作者机构：
[Qiu, Lin; Li, Youzhi; Zhou, Ailin; Ding, Wenbing; Xue, Jin; He, Hualiang] Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Peoples R China.;[Li, Youzhi; Zhou, Ailin; Ding, Wenbing] Hunan Prov Engn & Technol Res Ctr Biopesticide &, Changsha 410128, Peoples R China.;[Huang, Cong] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr,Shenzhen Branch, Genome Anal Lab,Guangdong Lab Lingnan Modern Agr, Shenzhen 518120, Peoples R China.;[Li, Xinwen; Zhang, Zhengbing; Li, Yi] Agr & Rural Dev Hunan Prov, Plant Protect & Inspect Stn, Changsha 410005, Peoples R China.
通讯机构：
[Li, Youzhi; Qiu, Lin] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Changsha, China
摘要：
A chromosome-level genome assembly for the rice pest, Chlorops oryzae, pinpoints molecular pathways that might contribute toward increased outbreaks for this important crop pest. Chlorops oryzae is a pest of rice that has caused severe damage to crops in major rice-growing areas in recent years. We generated a 447.60 Mb high-quality chromosome-level genome with contig and scaffold N50 values of 1.17 Mb and 117.57 Mb, respectively. Hi-C analysis anchored 93.22% scaffolds to 4 chromosomes. The relatively high expression level of Heat Shock Proteins (HSPs) and antioxidant genes in response to thermal stress suggests these genes may play a role in the environmental adaptability of C. oryzae. The identification of multiple pathways that regulate reproductive development (juvenile hormone, 20-hydroxyecdsone, and insulin signaling pathways) provides evidence that these pathways also play an important role in vitellogenesis and thus insect population maintenance. These findings identify possible reasons for the increased frequency of outbreaks of C. oryzae in recent years. Our chromosome-level genome assembly may provide a basis for further genetic studies of C. oryzae, and promote the development of novel, sustainable strategies to control this pest.
期刊：
Archives of Microbiology,2022年204(8):455 ISSN：0302-8933
通讯作者：
Liang, Z.;Liu, E.
作者机构：
[Liu, Erming; Xiao, Jiling] Hunan Agr Univ, Coll Plant Protect, Changsha 410125, Peoples R China.;[Xiao, Jiling; Yang, Ke; Liang, Zhihuai] Hunan Agr Biotechnol Res Inst, Changsha 410125, Peoples R China.;[Wei, Lin; Tang, Yanying] Hunan Plant Protect Inst, Changsha 410125, Peoples R China.;[Zhang, Yi] Hunan Rice Res Inst, Changsha 410125, Peoples R China.
通讯机构：
[Erming Liu] C;[Zhihuai Liang] H;College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Agricultural Biotechnology Research Institute, Changsha, China
作者机构：
[Cheng, Ju-E] College of Plant Protection, Hunan Agricultural University, Changsha, China, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha, China;[Zheng, Li-Min; Wang, Zhong-Yong; Hamid, Muhammad Rizwan; Dai, Jian-Ping; Du, Xiao-Hua; Chen, Li-Jie; Zhai, Zhong-Ying; Liu, Yong] Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha, China;[Su, Pin] College of Plant Protection, Hunan Agricultural University, Changsha, China;[Zhang, De-Yong] Hunan Hybrid Rice Research Center, Changsha, China;[Kong, Xiao-Ting] Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha, China, Long Ping Branch, Graduate School of Hunan University, Changsha, China
通讯机构：
[De-Yong Zhang] A;Affiliation Hunan Hybrid Rice Research Center, Changsha, China
关键词：
Seedlings;Soybeans;Tomatoes;Crops;Bacteria;Vegetable crops;Cucumber;Plant growth and development
摘要：
Photosynthetic bacteria are beneficial to plants, but knowledge of photosynthetic bacterial community dynamics in field crops during different growth stages is scarce. The factors controlling the changes in the photosynthetic bacterial community during plant growth require further investigation. In this study, 35 microbial community samples were collected from the seedling, flowering, and mature stages of tomato, cucumber, and soybean plants. 35 microbial community samples were assessed using Illumina sequencing of the photosynthetic reaction center subunit M (pufM) gene. The results revealed significant alpha diversity and community structure differences among the three crops at the different growth stages. Proteobacteria was the dominant bacterial phylum, and Methylobacterium, Roseateles, and Thiorhodococcus were the dominant genera at all growth stages. PCoA revealed clear differences in the structure of the microbial populations isolated from leaf samples collected from different crops at different growth stages. In addition, a dissimilarity test revealed significant differences in the photosynthetic bacterial community among crops and growth stages (P<0.05). The photosynthetic bacterial communities changed during crop growth. OTUs assigned to Methylobacterium were present in varying abundances among different sample types, which we speculated was related to the function of different Methylobacterium species in promoting plant growth development and enhancing plant photosynthetic efficiency. In conclusion, the dynamics observed in this study provide new research ideas for the detailed assessments of the relationship between photosynthetic bacteria and different growth stages of plants.
通讯机构：
[Xiaogang Li; Xiaogang Li Xiaogang Li Xiaogang Li] C;College of Plant Protection, Hunan Agricultural University, Southern Regional Collaborative Innovation Center for Grain and Oil Crops, Changsha, China
关键词：
citrus;carbendazim and epoxiconazole;digestion dynamics;risk assessment
通讯机构：
[Youzhi Li; Lin Qiu] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, China
摘要：
Chilo suppressalis is one of the most prevalent and damaging rice pests, causing significant economic losses each year. Chemical control is currently the primary method of controlling C. suppressalis. However, the indiscriminate use of chemical insecticides increases pest resistance, pollutes the environment and poses a significant health threat to humans and livestock, highlighting the need to find safer, more pest-specific and more effective alternatives to pest control. Plant-mediated RNA interference (RNAi) is a promising agricultural pest control method that is highly pest-specific and has less of an impact on the environment. Using multi-sgRNAs/Cas9 technology to delete Fatty acyl-CoA reductase (FAR) of C. suppressalis in the G0 generation, we show that downregulating FAR transcription may significantly increase the mortality rate and darken the epidermis of C. suppressalis compared with the control. Subsequently, we developed dsFAR transgenic rice lines using Agrobacterium-mediated genetic transformation and then screened three strains expressing dsFAR at high levels using transcriptional level analysis. Using transgenic rice stems, a laboratory feeding bioassay indicated that at least one line (L#10) displayed a particularly high level of insect resistance, with an insect mortality rate of more than 80%. In the field trials, dsFAR transgenic rice displayed high levels of resistance to C. suppressalis damage. Collectively, these results suggest the potential of a new environment-friendly, species-specific strategy for rice pest management.
通讯机构：
[Youzhi Li] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Agricultural University, Changsha, China
摘要：
BACKGROUND: The nutritional signaling pathway regulates an insect's size, development, and lifespan, as well as playing a vital role in reproduction. The insulin/insulin-like growth factor signaling (IIS) pathway plays a key role in the nutrition signaling pathway. As an integral component of the IIS pathway, insulin receptor (InR), a receptor tyrosine kinase, plays a role in the insulin pathway by controlling reproduction in many insect species. However, the precise molecular function of InR in non-model insect reproduction is poorly understood. METHODS: In our study, Chilo suppressalis, a well-known rice pest, was used as a molecular system to determine the role of InR in insect reproduction. Sequencing the InR gene of C. suppressalis, comparing the amino acid sequence-specific structure, and constructing a phylogenetic tree revealed that this gene has four main domains: ligand binding L domain, Furin-like region, fibronectin type III domains, and Tyrosine kinase catalytic domain, which were all highly conserved in insects. RESULTS: By characterizing the spatiotemporal expression profile of InR in different developmental stages and tissues, we found that InR gene expression was highest on the 3-day old in female pupae, 6th instar larvae, and fat body on the 6-day old in female pupae. InR gene expression may promote the molting and pupation of larvae and play a role in reproduction in the fat body. Furthermore, the RNA interference knockdown of InR dramatically reduced yolk deposition and blocked oocyte maturation. After suppression of InR, the expression of several other genes fluctuated to varying degrees. CONCLUSION: In conclusion, InR is vital to reproduction and is expected to become a new target for pest management.
期刊：
Pest Management Science,2022年78(11):4859-4870 ISSN：1526-498X
通讯作者：
Gong Chen<&wdkj&>Gong Chen Gong Chen Gong Chen
作者机构：
[Chen, Gong; Yuan, Ge-Ge; Yu, Huan; Du, Yuan-Wen] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.;[Chen, Gong; Yuan, Ge-Ge; Yu, Huan; Du, Yuan-Wen] Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China.;[Zhao, Lin-Chao] Tanghe Cty Agr & Rural Bur, Econ Crops Extens Dept, Nanyang, Peoples R China.;[Shi, Xiao-Bin] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha, Peoples R China.;[Chen, Wen-Chao] Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha, Peoples R China.
通讯机构：
[Gong Chen; Gong Chen Gong Chen Gong Chen] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, P. R. China<&wdkj&>College of Plant Protection, Hunan Agricultural University, Changsha, P. R. China
摘要：
Mycovirus-infected fungi can suffer from poor growth, attenuated pigmentation, and virulence. However, the molecular mechanisms of how mycoviruses confer these symptoms remain poorly understood. Here, we report a mycovirus Stemphylium lycopersici alternavirus 1 (SlAV1) isolated from a necrotrophic plant pathogen Stemphylium lycopersici that causes altered colony pigmentation and hypovirulence by specifically interfering host biosynthesis of Altersolanol A, a polyketide phytotoxin. SlAV1 significantly down-regulates a fungal polyketide synthase (PKS1), the core enzyme of Altersolanol A biosynthesis. PKS1 deletion mutants do not accumulate Altersolanol A and lose pathogenicity to tomato and lettuce. Transgenic expression of SlAV1 open-reading frame 3 (ORF3) in S. lycopersici inhibits fungal PKS1 expression and Altersolanol A accumulation, leading to symptoms like SlAV1-infected fungal strains. Multiple plant species sprayed with mycelial suspension of S. lycopersici or S. vesicarium strains integrating and expressing ORF3 display enhanced resistance against virulent strains, converting the pathogenic fungi into biocontrol agents. Hence, our study not only proves inhibiting a key enzyme of host phytotoxin biosynthesis as a molecular mechanism underlying SlAV1-mediated hypovirulence of Stemphylium spp., but also demonstrates the potential of mycovirus-gene integrated fungi as a potential biocontrol agent to protect plants from fungal diseases.
通讯机构：
[Liangying Dai] C;[Shaohong Qu] I;College of Plant Protection, Hunan Agricultural University, Changsha, China<&wdkj&>Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
摘要：
BACKGROUND: Biotechnologists seeking to develop marker-free transgenic plants have established co-transformation methods. For co-transformation using mixed Agrobacterium strains, the mix ratio of Agrobacterium strains and selection scheme may influence co-transformation frequency. This study used fluorescent GFP and RFP markers to compose different selection schemes for observation of the selective dynamics of transformed rice cells and to investigate the factors affecting co-transformation efficiency. METHODS AND RESULTS: We utilized GFP and RFP markers in co-transformation and tested the combinations of an antibiotic-selectable vector (pGFP-HPT) and a single RFP vector (pRFP) and of two antibiotic-selectable vectors (pGFP-HPT and pRFP-HPT) in rice. The pGFP-HPT/pRFP combination resulted in 70.9% to 81.2% of co-transformation frequencies while lower frequencies (56.6% on average) were obtained with the pGFP-HPT/pRFP-HPT combination. Based on GFP/RFP segregation patterns, 55% of the pGFP-HPT/pRFP co-transformants contained unlinked T-DNAs and segregated single RFP progeny, which simulated the selection process of marker-free transgenic plants that carry an actual gene of interest. Transgene expression levels in the rice lines varied as revealed by RT-PCR, and tandem-linked T-DNAs were detected in co-transformants, suggesting that transgene expression might be affected by duplicated T-DNA structures. CONCLUSION: Co-transformation via mixed Agrobacterium strains is feasible, and approximately 55% of the pGFP-HPT/pRFP co-transformants contained unlinked T-DNAs and segregated single RFP progeny. The pGFP-HPT/pRFP and the pGFP-HPT/pRFP-HPT vector combinations showed distinctive selective dynamics of transformed rice cells, suggesting that co-transformation efficiency depends on both vector system and selection scheme.
通讯机构：
[Siqiao Tan] C;[Wei Zhou] H;College of Information and Intelligence, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Provincial Engineering and Technology Research Center for Agricultural Big Data Analysis and Decision-Making, Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, 410128, China
摘要：
In crops, RNA editing is one of the most important post-transcriptional processes in which specific cytidines (C) in virtually all mitochondrial protein-coding genes are converted to uridines (U). Despite extensive recent research in RNA editing, exploring all of the C-to-U editing events efficiently on the genomic scale remains challengeable. Developing accurate prediction methods for the detection of RNA editing sites would dramatically reduce experimental determination. Therefore, we propose a novel method, iPReditor-CMG (improved predictive RNA editor for crop mitochondrial genomes), to predict crop mitochondrial editing sites using genome sequence and an optimised support vector machine (SVM). We first selected three mitochondrial genomes with known RNA editing sites from Arabidopsis thaliana, Brassica napus and Oryza sativa, released by NCBI, as the training and test sets. The genes and their transcripts from self-sequenced tobacco mitochondrial ATPase were selected as the validation set. The iPReditor-CMG first coded the genome sequences as numerical vectors and then performed an efficient feature selection on the high-dimensional feature space, where the SVM was employed in feature selection and following modelling. The average independent prediction accuracy of intraspecific editing sites across three species was 0.85, and up to 0.91 in A. thaliana, which outperformed the reference models. For the interspecific independent prediction, the prediction accuracy between dicotyledons was 0.78 and the accuracy between dicotyledons and monocotyledons was 0.56, which implies that there might be similarity in the C-to-U editing mechanism in close relatives. Finally, the best model was identified with an independent test accuracy of 0.91 and an AUC of 0.88, which suggested that five unreported feature sequences, i.e. TGACA, ACAAC, GTAGA, CCGTT and TAACA, are closely associated with the editing phenomenon. Multiple tests supported that the iPReditor-CMG could be effectively applied to predict editing sites in crop mitochondria, which may further contribute to understanding the mechanisms of site editing and post-transcriptional events in crop mitochondria.
摘要：
In this study, two pyridine-degrading strains namely Enterobacter cloacae complex sp. BD17 and Enterobacter sp.BD19 were isolated from the aerobic tank of a pesticide wastewater treatment plant. The mixed bacteria H4 composed of BD17 and BD19 at a ratio of 1:1 was immobilized by Solidago canadensis L. stem biochar with a dosage of 2g·L(-1). The highest pyridine removal rate of 91.70% was achieved by the immobilized H4 at an initial pyridine concentration of 200mg·L(-1), pH of 7.0, temperature of 28°C and salinity of 3.0% within 36h. The main intermediates of pyridine degradation by BD17 were pyridine-2-carboxamide, 2-aminopropanediamide, and 2-aminoacetamide, while 2-picolinic acid, isopropyl acetate, isopropyl alcohol, and acetaldehyde were identified with BD19 by adopting GC-MS technique. Interestingly, there was a possibility of totally mineralization of pyridine and the corresponding degradation pathways of BD17 and BD19 were revealed for the first time.
摘要：
A novel virus, Botryosphaeria dothidea bipartite mycovirus 1 (BdBMV1), was isolated from the plant-pathogenic fungus Botryosphaeria dothidea strain HNDT1, and the complete nucleotide sequence of its genome was determined. BdBMV1 consists of two genomic segments. The first segment is 1,976 bp in length and contains a single open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRp) (68.95 kDa). The second segment is 1,786 bp in length and also contains a single ORF encoding a hypothetical protein of 35.19 kDa of unknown function. Based on the sequence of its RdRp, BdBMV1 is phylogenetically related to several other unclassified dsRNA mycoviruses, including Cryphonectria parasitica bipartite mycovirus 1 (CpBV1), and has a distant relationship to members of the family Partitiviridae.
通讯机构：
[Qian Zhou] H;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, People’s Republic of China
摘要：
A novel mycovirus belonging to the proposed family “Fusariviridae” was discovered in Alternaria solani by sequencing a cDNA corresponding to double-stranded RNA extracted from this phytopathogenic fungus. The virus was tentatively named “Alternaria solani fusarivirus 1” (AsFV1). AsFV1 has a single-stranded positive-sense (+ssRNA) genome of 6845 nucleotides containing three open reading frames (ORFs) and a poly(A) tail. The largest ORF, ORF1, encodes a large polypeptide of 1,556 amino acids (aa) with conserved RNA-dependent RNA polymerase and helicase domains. The ORF2 and ORF3 have overlapping regions, encoding a putative protein of 522 amino acids (aa) and a putative protein of 105 amino acids (aa), respectively, both of unknown function. A multiple sequence alignment and phylogenetic analysis revealed that AsFV1 could be a new member of the “Fusariviridae”. This is the first report of the full-length nucleotide sequence of a fusarivirus that infects Alternaria solani.
