期刊：
Journal of Functional Foods,2024年113:106049 ISSN：1756-4646
通讯作者：
Huansheng Yang<&wdkj&>Mingzhi Zhu
作者机构：
[Qiye Wang; Xianglin Zeng] Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China;[Bohao Shang; Yilong Li; Fang Zhou; Mingzhi Zhu] Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China;Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;[Zhaobin Wang] Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China<&wdkj&>National Center of Technology Innovation for Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
通讯机构：
[Huansheng Yang] H;[Mingzhi Zhu] K;Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China<&wdkj&>Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
摘要：
Lipid deposition has emerged as a looming challenge worldwide. Previous studies have confirmed the fat-lowering effect of tea polyphenols (TP) in mice, but the underlying mechanism remains elusive. Furthermore, it remains unclear whether TP has the same effect in larger animals. Herein, we simultaneously investigated the regulatory effects of TP on lipid deposition in rats and Ningxiang pigs. The results showed that TP could effectively reduce fat accumulation in rats on a high-fat diet (HFD). As expected, TP supplementation led to a reduction in backfat thickness, fat rate, and subcutaneous fat cell size in Ningxiang pigs. Additionally, TP attenuated gut microbiota dysbiosis by enhancing microbial diversity and increasing the relative abundance of Bacteroides and Akkermansia in both models. Collectively, our results suggest that TP may reduce fat accumulation in rats and Ningxiang pigs by regulating gut microbiota. Therefore, TP could potentially serve as a functional agent for improving fat deposition in humans and Ningxiang pigs.
作者机构：
[Liu, Dongbo; Zhang, Zhixu] State Key Lab Subhlth Intervent Technol, Changsha 410128, Hunan, Peoples R China.;[Liu, Dongbo; Zhang, Zhixu] Hunan Agr Univ, Coll Hort, Changsha 410128, Hunan, Peoples R China.;[Qin, Dan] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Guo, Xin] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;[Lin, Haiyan] Natl Res Ctr Engn Technol Utilizat Ingredients Bot, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Guo, X ] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.
关键词：
Adsorption;Carbon dots;Pb(II);Starch
摘要：
The adsorption removal of lead (Pb) ions has become a crucial area of research due to the potential health hazards associated with Pb contamination. Developing cost-effective adsorbents for the removal of Pb(II) ions is significantly important. Hence, a novel fluorescent starch-based hydrogel (FSH) using starch (ST), cellulose nanofibrils (CN), and carbon dots (CD) was fabricated for simultaneous adsorption and detection of Pb(II). A comprehensive characterization of FSH, including its morphological features, chemical composition, and fluorescence characteristics, was conducted. Notably, FSH exhibited a maximum theoretical adsorption capacity of 265.9mg/g, which was 13.0 times higher than that of pure ST. Moreover, FSH was employed as a fluorescent sensor for Pb(II) determination, achieving a limit of detection (LOD) of 0.06μg/L. An analysis was further performed to investigate the adsorption and detection mechanisms of Pb(II) utilizing FSH. This study provides valuable insights into the production of a novel cost-effective ST-based adsorbent for the removal of Pb(II) ions.
摘要：
Purple tea, renowned for its anthocyanin content and distinctive purple hue, has gained prominence. The anthocyanin content in purple tea can exceed three times that of traditional green-leaf tea. Purple tea harbors various anthocyanins, implicating intricate pathways of biosynthesis and transcriptional regulation. Concurrently, owing to its distinctive chemical composition, the processing of purple tea may be constrained, potentially influencing the sensory attributes and flavor profile of the tea. The richness of anthocyanins in purple tea has yielded potential health benefits, including antioxidative and anti-cancer properties, rendering purple tea a sought-after commodity in the tea market. However, current research on purple tea remains incomplete, including indistinct networks of anthocyanin biosynthesis and regulatory mechanisms, incomplete chemical characterization, and a need for comprehensive investigations into its biological activities. The limited research foundation has greatly reduced the popularity and consumption of purple tea. This paper aims to provide an overview of recent advancements in the biosynthesis and regulation of anthocyanins, as well as the chemical compositions, processing, and health benefits of purple tea. This review will provide the groundwork for future efforts in the selection and innovation of purple tea germplasm, purple tea processing, and the expansion of the market for purple tea consumption.
摘要：
Uncaria rhynchophylla is an evergreen vine plant, belonging to the Rubiaceae family, that is rich in terpenoid indole alkaloids (TIAs) that have therapeutic effects on hypertension and Alzheimer's disease. GATA transcription factors (TF) are a class of transcription regulators that participate in the light response regulation, chlorophyll synthesis, and metabolism, with the capability to bind to GATA cis-acting elements in the promoter region of target genes. Currently the charactertics of GATA TFs in U. rhynchophylla and how different light qualities affect the expression of GATA and key enzyme genes, thereby affecting the changes in U. rhynchophylla alkaloids have not been investigated. In this study, 25 UrGATA genes belonging to four subgroups were identified based on genome-wide analysis. Intraspecific collinearity analysis revealed that only segmental duplications were identified among the UrGATA gene family. Collinearity analysis of GATA genes between U. rhynchophylla and four representative plant species, Arabidopsis thaliana, Oryza sativa, Coffea Canephora, and Catharanthus roseus was also performed. U. rhynchophylla seedlings grown in either red lights or under reduced light intensity had altered TIAs content after 21 days. Gene expression analysis reveal a complex pattern of expression from the 25 UrGATA genes as well as a number of key TIA enzyme genes. UrGATA7 and UrGATA8 were found to have similar expression profiles to key enzyme TIA genes in response to altered light treatments, implying that they may be involved in the regulation TIA content. In this research, we comprehensively analyzed the UrGATA TFs, and offered insight into the involvement of UrGATA TFs from U. rhynchophylla in TIAs biosynthesis.
期刊：
Current Plant Biology,2024年38:100330 ISSN：2214-6628
通讯作者：
Huan Zhao<&wdkj&>Qi Tang
作者机构：
[Yingying Shao; Detian Mu; Zhiguo Lu; Yu Zhou; Ying Lu; Lina Zhu; Yao Zhang; Xinghui Liu; Qi Tang] College of Horticulture, National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Hunan Agricultural University, Changsha 410128, China;[Limei Pan; Ya Qin] Guangxi Key Laboratory of for High-Quality Formation and Utilization of Dao-Di Herbs, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, China;[Huan Zhao] School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China;CSIRO Agriculture and Food, Canberra, ACT 2601, Australia;[Deyou Qiu] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
通讯机构：
[Huan Zhao] S;[Qi Tang] C;College of Horticulture, National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Hunan Agricultural University, Changsha 410128, China<&wdkj&>School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
摘要：
Uncaria rhynchophylla (Gouteng), as an evergreen woody vine belong to Rubiaceae family, is a traditional medicinal herb in China. Its terpenoid indole alkaloids (TIAs), which have good antidepressant and combined therapeutic effects on Alzheimer's disease, have attracted widespread attention. However, the content of TIAs is relatively low in U.rhynchophylla, which is unable to meet the growing market demand. The basic helix loop-helix (bHLH) transcription factor family exists in all three eukaryotic kingdoms and can participate in regulating secondary metabolite pathways. So far, there has been no comprehensive analysis of the bHLH gene in U. rhynchophylla, and their role in TIAs is almost unknown. In this study, a total of 171 UrbHLH genes (UrbHLHs) were unevenly distributed on 22 chromosomes and divided into 23 subfamilies. In addition, the physicochemical properties of UrbHLHs were analyzed. Most UrbHLHs in each subgroup had similar gene structures and conserved motifs. Intraspecific collinearity analysis showed that UrbHLH1 may be related to the biosynthesis of TIAs. Subcellular localization experiments revealed that UrbHLH1 is located in the nucleus; Dual luciferase reporter gene analysis (Dual-LUC) showed that UrbHLH1 could activate the expression of UrG10H and Ur10HGO in the TIAs synthesis pathway of U. rhynchophylla. Finally, using yeast one hybrid (Y1H) it was found that the promoter regions of these two genes both have E-box binding elements, which can be bound by UrbHLH1 and produced strong interactions. Therefore, UrbHLH1 may participate in the synthesis of TIAs pathway. In conclusion, this study provides foundation data on the role of UrbHLH transcription factors in regulating TIAs of U. rhynchophylla.
摘要：
Increased planting densities boost crop yields. A compact plant architecture facilitates dense planting. However, the mechanisms regulating compact plant architecture in cucurbits remain unclear. In this study, we identified a cucumber (Cucumis sativus) compact plant architecture (cpa1) mutant from an ethyl methane sulfonate (EMS)-mutagenized library that exhibited distinctive phenotypic traits, including reduced leaf petiole angle and leaf size. The candidate mutation causes a premature stop codon in CsaV3_1G036420, which shares similarity to Arabidopsis HOOKLESS 1 (HLS1) encoding putative histone N-acetyltransferase (HAT) protein and was named CsHLS1. Consistent with the mutant phenotype, CsHLS1 was predominantly expressed in leaf petiole bases and leaves. Constitutive overexpressing CsHLS1 in cpa1 restored the wild-type plant architecture. Knockout of CsHLS1 resulted in reduces leaf petiole angle and leaf size and as well as decreased acetylation levels. Furthermore, CsHLS1 directly interacted with CsSCL28 and negatively regulated compact plant architecture in cucumber. Importantly, CsHLS1 knockout increased the photosynthesis rate and leaf nitrogen in cucumbers, thereby maintaining cucumber yield at normal density. Overall, our research provides valuable genetic breeding resource and gene target for creating a compact plant architecture for dense cucumber planting.
作者：
Kun Gao;Dejun Li;Ze Luo*;Qiulong Hu;Shuzhen Feng;...
期刊：
Agronomy,2024年14(3):467- ISSN：2073-4395
通讯作者：
Ze Luo
作者机构：
College of Horticulture, Hunan Agricultural University, Changsha 410128, China;Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;[Kun Gao; Dejun Li] Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;These authors contributed equally to this work.;Author to whom correspondence should be addressed.
通讯机构：
[Ze Luo] C;College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Soil microbial activity is generally limited by the availability of carbon (C), nitrogen (N), or phosphorus (P) in agricultural ecosystems. Soil ecoenzymatic activity (EEA), ecoenzymatic stoichiometry (EES), and vector characteristics were examined to assess microbial nutrient limitation. Investigating soil microbial nutrient limitation can provide insight into nutrient cycling in tea plantations with different tea cultivars. However, the dynamics of different tea cultivars on soil microbial nutrient limitations and their effect on tea quality remains poor. To address this issue, soil and plant samples were collected from a tea plantation cultivating five representative tea cultivars in Hunan Province, China. Baojing Huangjincha No. 1 (HJC1) and Huangjincha No. 2 (HJC2) were the extra early-sprouting cultivars, Zhuyeqi (ZYQ) and Zijuan (ZJ) were the middle-sprouting cultivars, and Zhenghedabai (ZHDB) was the late-sprouting cultivar, respectively. The results indicated that differences in EEA and EES were significant among five treatments. Notably, ZYQ and ZJ exhibited markedly lower activities of carbon (C), nitrogen (N), and phosphorus (P) acquiring enzymes compared to HJC1 and HJC2, whereas ZHDB showed significantly higher ecoenzymatic activities. Despite a general limitation in C and P for soil microorganisms across all cultivars (VL ranging from 1.42 to 1.59 and VA ranging from 58.70° to 62.66°), the degree of microbial nutrient limitation varied. Specifically, ZYQ experienced a pronounced P limitation (VA = 62.66°, N:P enzyme = 0.52), as evidenced by increased vector angles and decreased N:P enzyme values. Although C limitation was most pronounced in ZYQ (VL = 1.59), it did not significantly differ among the cultivars. These findings suggest that tea cultivars can influence the P limitation of microbial communities. Further analysis revealed that microbial nutrient limitations might adversely affect tea quality via impeding enzyme secretion. This study highlights the critical role of nutrient cycling within the soil-microorganism-plant ecosystem and emphasizes the influence of soil microbial nutrient limitations on tea quality within tea plantations. It is recommended that in the management of tea plantation fertilization, managers need to consider the influence of cultivars and develop specialized cultivar fertilizers.
作者机构：
[Lu, Ying; Zheng, Yajie; Xiao, Tian; Yang, Li; Nie, Guang] National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;[Xiao, Tian; Yang, Li; Nie, Guang] College of Horticulture, Hunan Agricultural University, Changsha 410128, China;[Zhong, Xiaohong; Yang, Fan] College of Horticulture, Hunan Agricultural University, Changsha 410128, China;[Jin, Xiue] Hubei Institute of Veterinary Drug Control, Wuhan 430000, China;[Peng, Xiaoying] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Lu, Ying; Zheng, Yajie] C;[Wang, Jun] H;Hubei Institute of Veterinary Drug Control, Wuhan 430000, China. Electronic address:;College of Horticulture, Hunan Agricultural University, Changsha 410128, China. Electronic address:
摘要：
The object of this study was to trace TwHf-derived toxins in raw honey and clarify their acute toxic effect related to the addition of honey or sugars. TwHf flowers, raw honey from TwHf planting base and from beekeepers in high-risk area were detected using LC-MS/MS. The results revealed five target toxins were detected in TwHf flowers; only celastrol was detected in one raw honey sample, as a food safety risk factor, celastrol had been traced back to TwHf flowers from raw honey. In a series of acute toxic tests on zebrafish, toxification effects were observed when honey, mimic honey or sugar was mixed with toxins. The degree of toxicity varied among various sugar-based solutions. At the same mass concentration, they follow this order: raw honey/mimic honey > glucose > fructose. The main toxic target organs of triptolide and celastrol with honey were the heart and liver.
通讯作者：
Jian'an Huang<&wdkj&>Zhonghua Liu<&wdkj&>Jian'an Huang Jian'an Huang Jian'an Huang<&wdkj&>Zhonghua Liu Zhonghua Liu Zhonghua Liu
作者机构：
[Hongzhe Zeng; Kuofei Wang; Changwei Liu; Jie Ouyang; Shuai Wen; Fang Zhou; Jingyi Tang; Wenwen Fang; Lin Yue; Jian'an Huang; Zhonghua Liu; Hongzhe Zeng Hongzhe Zeng Hongzhe Zeng; Kuofei Wang Kuofei Wang Kuofei Wang; Changwei Liu Changwei Liu Changwei Liu; Jie Ouyang Jie Ouyang Jie Ouyang; Shuai Wen Shuai Wen Shuai Wen; Fang Zhou Fang Zhou Fang Zhou; Jingyi Tang Jingyi Tang Jingyi Tang; Wenwen Fang Wenwen Fang Wenwen Fang; Lin Yue Lin Yue Lin Yue; Jian'an Huang Jian'an Huang Jian'an Huang; Zhonghua Liu Zhonghua Liu Zhonghua Liu] Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, China
通讯机构：
[Jian'an Huang; Zhonghua Liu; Jian'an Huang Jian'an Huang Jian'an Huang; Zhonghua Liu Zhonghua Liu Zhonghua Liu] K;Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha, China
摘要：
(1) This study may be the first study to evaluate the effects of oolong tea with different storage time in db/db T2DM mice.(2) All three aged oolong tea extracts (2001, 2011, 2020) can effectively alleviate type 2 diabetes mellitus (T2DM), and 2011 aged oolong tea extract exhibited relatively optimal ability to alleviate T2DM.(3) With storage time of oolong tea increasing, oolong tea’s ability to inhibit fat ectopic deposition in liver increases and the ability to activate INSR/IRS/PI3K/AKT/GSK‐3β/GYS‐mediated hepatic glycogen synthesis signaling pathway decreases. Abstract An important concern for tea consumers is whether appropriate storage can effectively improve the ability of oolong tea to alleviate type 2 diabetes mellitus (T2DM). In this study, the antidiabetic effects of three aged oolong tea (2001, 2011, and 2020) extracts (aged TEs) in db/db T2DM mice were investigated and compared for the first time. The results showed that aged oolong tea extracts (aged TEs) alleviated the abnormal fasting blood glucose levels, lipid profiles, insulin resistance, and reduced ectopic fat deposition in liver. Moreover, aged TEs alleviated T2DM‐related tissue damage and activated insulin receptor (INSR)/insulin receptor substrate (IRS)/phosphatidylinositol‐3 kinase (PI3K)/phosphatidylinositol‐3 kinase (AKT)/glycogen synthase kinase 3 beta (GSK‐3β)/glycogen synthase (GYS)‐mediated hepatic glycogen synthesis signaling pathway. In addition, aged TEs altered gut microbiota composition and gut metabolite profiles associated with T2DM. Together, all three aged TEs (400 mg/kg/day in mice, a human equivalent dose of 7 g/60 kg/day), especially 2011, can effectively alleviate T2DM, and proper storage can effectively improve the ability of oolong tea to alleviate T2DM.
摘要：
The species and contents of a-dicarbonyls in commercial black tea were examined, along with the effects of the manufacturing process and drying temperature on the formation of a-dicarbonyls. Ten a-dicarbonyls were quantified in commercial and in -process black tea samples by using UPLC-MS/MS and their derived quinoxalines. The a-dicarbonyls content in commercial black tea decreased significantly (p < 0.05) in the following order: 3deoxyglucosone > glucosone > 3-deoxypentosone = threosone > galactosone >= methylglyoxal = glyoxal >= 3deoxygalactosone = 3-deoxythreosone = diacetyl. Except for 3-deoxyglucosone and 3-deoxygalactosone, a further eight a-dicarbonyls were identified in all manufacturing steps of black tea. Except for the drying step, the rolling and fermenting played important roles in the formation of a-dicarbonyls. The total contents of a-dicarbonyls in black tea infusion ranged from 16.48 to 75.32 mu g/g based on our detected ten a-dicarbonyls.
作者机构：
[Le Huang; Huimin Zhang; Jiayi Tang; Na Li; Alessandra Gentile; Haojie Yin; Suming Dai; Yan Jin; Xiaopeng Lu; Hongbin Liu; Chouyu Miao] National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China;[Alessandra Gentile] Department of Agriculture and Food Science, University of Catania, Catania, 95123, Italy;[Ling Zhang] Agriculture and Rural Bureau of Mayang Miao Autonomous County, Huaihua, China;[Ling Sheng] National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, 410128, China. shengling0629@163.com
通讯机构：
[Ling Sheng] N;National Center for Citrus Improvement Changsha, College of Horticulture, Hunan Agricultural University, Changsha, China
摘要：
Anthocyanins are the most important compounds for nutritional quality and economic values of blood orange. However, there are few reports on the pre-harvest treatment accelerating the accumulation of anthocyanins in postharvest blood orange fruit. Here, we performed a comparative transcriptome and metabolomics analysis to elucidate the underlying mechanism involved in seasonal drought (SD) treatment during the fruit expansion stage on anthocyanin accumulation in postharvest ‘Tarocco’ blood orange fruit. Our results showed that SD treatment slowed down the fruit enlargement and increased the sugar accumulation during the fruit development and maturation period. Obviously, under SD treatment, the accumulation of anthocyanin in blood orange fruit during postharvest storage was significantly accelerated and markedly higher than that in CK. Meanwhile, the total flavonoids and phenols content and antioxidant activity in SD treatment fruits were also sensibly increased during postharvest storage. Based on metabolome analysis, we found that substrates required for anthocyanin biosynthesis, such as amino acids and their derivatives, and phenolic acids, had significantly accumulated and were higher in SD treated mature fruits compared with that of CK. Furthermore, according to the results of the transcriptome data and weighted gene coexpression correlation network analysis (WGCNA) analysis, phenylalanine ammonia-lyase (PAL3) was considered a key structural gene. The qRT-PCR analysis verified that the PAL3 was highly expressed in SD treated postharvest stored fruits, and was significantly positively correlated with the anthocyanin content. Moreover, we found that other structural genes in the anthocyanin biosynthesis pathway were also upregulated under SD treatment, as evidenced by transcriptome data and qRT-PCR analysis. The findings suggest that SD treatment promotes the accumulation of substrates necessary for anthocyanin biosynthesis during the fruit ripening process, and activates the expression of anthocyanin biosynthesis pathway genes during the postharvest storage period. This is especially true for PAL3, which co-contributed to the rapid accumulation of anthocyanin. The present study provides a theoretical basis for the postharvest quality control and water-saving utilization of blood orange fruit.
摘要：
BACKGROUND: Dichlorvos (DDVP), as a highly effective insecticide, is widely used in agricultural production. However, DDVP residue in foodstuffs adversely affects human health. Conventional instrumental analysis can provide highly sensitive and accurate detection of DDVP, while the need of bulky and expensive equipment limits their application in resource-poor areas and on-site detection. Therefore, the development of easily portable sensing platforms for convenient, rapid and sensitive quantification of DDVP is very essential for ensuring food safety. RESULT: A portable colorimetric sensing platform for rapid and sensitive quantification of DDVP is developed based on nanozyme-participated highly efficient chromogenic catalysis. The Fe-Mn bimetallic oxide (FeMnOx) nanozyme possesses excellently oxidase-like activity and can efficiently catalyze oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB) into a blue oxide with a very low Michaelis constant (K(m)) of 0.0522mM. The nanozyme-catalyzed chromogenic reaction can be mediated by DDVP via inhibiting the acetylcholinesterase (AChE) activity. Thus, trace DDVP concentration-dependent color evolution is achieved and DDVP can be sensitively detected by spectrophotometry. Furthermore, a smartphone-integrated 3D-printed miniature lightbox is fabricated as the colorimetric signal acquisition and processing device. Based on the FeMnOx nanozyme and smartphone-integrated lightbox system, the portable colorimetric sensing platform of DDVP is obtained and it has a wide linear range from 1 to 3000ngmL(-1) with a low limit of detection (LOD) of 0.267ngmL(-1) for DDVP quantification. SIGNIFICANCE: This represents a new portable colorimetric sensing platform that can perform detection of DDVP in foodstuffs with simplicity, sensitivity, and low cost. The work not only offers an alternative to rapid and sensitive detection of DDVP, but also provides a new insight for the development of advanced sensors by the combination of nanozyme, 3D-printing and information technologies.
期刊：
Postharvest Biology and Technology,2024年212:112846 ISSN：0925-5214
通讯作者：
Yihe Yu
作者机构：
[Yihan Yue; Xiaochun Zhao; Yadan Sun; Qiaofang Shi; Yihe Yu] College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, Henan 471023, China;[Shengdi Yang] College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China
通讯机构：
[Yihe Yu] C;College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, Henan 471023, China
摘要：
Rachis browning adversely affects the appearance quality and commodity value, thus paring down consumer acceptability. A certain cognition on browning mechanism has been made after years of research. But how to modulate transcription of downstream genes via mechanisms mediated by histone acetylation modification in this regard is not yet clear. In this study, a histone deacetylase, VlSRT1, of grape rachis was found to contribute to the transcriptional modification of histone H3 acetylation in ethylene signal transduction. Physical interaction between VlSRT1 and VlERF95 was confirmed both in vivo and in vitro. In the presence of 1-MCP, increased VlSRT1 brings about a reduction of histone H3ac abundance at the site adjacent to the VlERF95-binding site in the promoter of VlERF95-targeted genes VlACS5 and VlPAO1, and negatively regulates ethylene biosynthesis and Chlorophyll degradation. This indicates that VlSRT1 might be recruited by VlERF95 into the promoters of VlACS5 and VlPAO1 to form a complex, which impairs the transcriptional activation of VlACS5 and VlPAO1, thereby retarding the progression of browning and senescence in rachis. Chromatin immunoprecipitation of acetylated histone H3 on the promoters of VlACS5 and VlPAO1 further validated the effect of ethylene on acetylation levels. Our results enrich new mechanisms of epigenetic regulation in ethylene signal transduction in grape and provide new research ideas for understanding the occurrence of rachis browning and the anti-browning role of 1-MCP in preserving rachis freshness after harvest.
作者机构：
[Yao Zhou; Weisheng Wu; Ying Sun; Yiyu Shen; Lianzhen Mao; Yunhua Dai; Bozhi Yang; Zhoubin Liu] Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China
通讯机构：
[Bozhi Yang; Zhoubin Liu] E;Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China<&wdkj&>Engineering Research Center of Education Ministry for Germplasm Innovation and Breeding New Varieties of Horticultural Crops, Key Laboratory of Vegetable Biology of Hunan Province, College of Horticulture, Hunan Agricultural University, Changsha, China
关键词：
Chili pepper;Continuous photoperiod;Anthocyanin biosynthesis;Blue light
摘要：
Different metabolic compounds give pepper leaves and fruits their diverse colors. Anthocyanin accumulation is the main cause of the purple color of pepper leaves. The light environment is a critical factor affecting anthocyanin biosynthesis. It is essential that we understand how to use light to regulate anthocyanin biosynthesis in plants. Pepper leaves were significantly blue–purple only in continuous blue light or white light (with a blue light component) irradiation treatments, and the anthocyanin content of pepper leaves increased significantly after continuous blue light irradiation. This green-to-purple phenotype change in pepper leaves was due to the expression of different genes. We found that the anthocyanin synthesis precursor-related genes PAL and 4CL, as well as the structural genes F3H, DFR, ANS, BZ1, and F3’5’H in the anthocyanin synthesis pathway, had high expression under continuous blue light irradiation. Similarly, the expression of transcription factors MYB1R1-like, MYB48, MYB4-like isoform X1, bHLH143-like, and bHLH92-like isoform X3, and circadian rhythm-related genes LHY and COP1, were significantly increased after continuous blue light irradiation. A correlation network analysis revealed that these transcription factors and circadian rhythm-related genes were positively correlated with structural genes in the anthocyanin synthesis pathway. Metabolomic analysis showed that delphinidin-3-O-glucoside and delphinidin-3-O-rutinoside were significantly higher under continuous blue light irradiation relative to other light treatments. We selected 12 genes involved in anthocyanin synthesis in pepper leaves for qRT-PCR analysis, and the accuracy of the RNA-seq results was confirmed. In this study, we found that blue light and 24-hour irradiation together induced the expression of key genes and the accumulation of metabolites in the anthocyanin synthesis pathway, thus promoting anthocyanin biosynthesis in pepper leaves. These results provide a basis for future study of the mechanisms of light quality and photoperiod in anthocyanin synthesis and metabolism, and our study may serve as a valuable reference for screening light ratios that regulate anthocyanin biosynthesis in plants.
摘要：
Temperature is an important environmental factor affecting plant color, and different temperatures have distinct effects on plant leaf color changes. In order to explore the effects of different temperatures on plant color, we measured the physiological characteristics, transcriptome, and metabolome of the pepper leaf yellowing mutant yl1 and its wild type 6421 under different temperature conditions. Results showed that high temperature treatment compared with the normal temperature treatment significantly increased the contents of chlorophyll a, chlorophyll b, and carotenoids in yl1. At the same time, a total of 28 carotenoid pathway metabolites were detected under three temperature conditions. Among them, beta-carotene, zeaxanthin, violaxanthin, neoxanthin, lutein, and antheraxanthin were higher in pepper leaves. The expression levels of ZEP, bHLH104, MYB1R1, and MYB1R1-like were significantly increased under high temperature compared with under normal temperature, while the expression level of crtZ-2 and VDE were significantly decreased. Co-expression network analysis showed that VDE was positively correlated with the bHLH71-like and negatively correlated with MYB1R1-like and bHLH104. These results led to significant differences in the amount of zeaxanthin and antheraxanthin accumulated at different temperatures, which may be the reason for the color change of pepper leaves. However, under the normal and low temperature treatment, it is the opposite, resulting in leaf yellowing. This study provides a reference for further research on the mechanism underlying the impact of temperature on carotenoid metabolism in pepper leaves.
作者机构：
College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China;Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China;Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China;Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China;[Qiuyun Wu; Qi Wu; Yuxiao Tian; Chunyan Zhou; Shuxiang Mao; Junwei Wang; Ke Huang] College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China
通讯机构：
[Junwei Wang; Ke Huang] C;College of Horticulture, Hunan Agricultural University, Changsha, Hunan 410128, China<&wdkj&>Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Vegetable Biology of Hunan Province, Changsha, Hunan 410128, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha, Hunan 410128, China
关键词：
Brassica oleracea var. italica;Myrosinase;BoMY;Sulforaphane;Selenite treatment
摘要：
Sulforaphane, a naturally specialized metabolite, plays significant roles in human disease prevention and plant defense. Myrosinase (MY) is a key gene responsible for the catalysis of sulforaphane formation, but the molecular mechanisms through which MY regulates sulforaphane biosynthesis in plants remains largely unknown. Here, we discovered that the change of sulforaphane content in broccoli sprouts caused by exogenous selenite treatments is positively related to BoMY expression. BoMY overexpression in the Arabidopsis thaliana tgg1 mutants could dramatically increase myrosinase activity and sulforaphane content in the rosette leaves of 35S::BoMY/tgg1 and rescue its phenotypes. Moreover, an obvious increase of myrosinase activity and sulforaphane content was displayed in transgenic BoMY-overexpressed broccoli lines. In addition, a 2 033 bp promoter fragment of BoMY was isolated. Yeast one-hybrid (Y1H) library screening experiment uncovered that one bHLH transcription factor, BoFAMA, could directly bind to BoMY promoter to activate its expression, which was further evidenced by Y1H assay and dual-luciferase reporter assay. BoFAMA is a selenite-responsive transcription factor that is highly expressed in broccoli leaves; its protein is solely localized to nucleus. Additionally, genetic evidence suggested that the knockdown of FAMA gene in Arabidopsis thaliana could significantly decrease sulforaphane yield by inhibiting the expression of myrosinase genes. Interestingly, exogenous selenite supply could partially restore the low level of sulforaphane content in transgenic Arabidopsis FAMA-silencing plants. Our findings uncover a novel function of FAMA-MY module in the regulation of selenite-mediated sulforaphane synthesis and provide a new insights into the molecular mechanism by which selenite regulates the accumulation of sulforaphane in plants.
作者机构：
Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China;College of Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China;[Zhou, Li; Song, Rong] Hunan Institute of Agricultural Environment and Ecology, Hunan Academy of Agricultural Sciences , Changsha 410125, Hunan, China;[Zhong, Xiaohong] College of Horticulture, Hunan Agricultural University , Changsha 410128, Hunan, China;National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Agricultural University , Changsha 410128, Hunan, China
通讯机构：
[Peng Huang] C;[Jianguo Zeng] H;Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Agricultural University , Changsha 410128, Hunan, China<&wdkj&>College of Animal Science and Technology, Hunan Agricultural University , Changsha 410128, Hunan, China
关键词：
High protein diet;L-Theanine;Neurotransmitters;Nutritional intervention
摘要：
Excessive protein intake causes liver and brain damage and neurotransmitter disorders, thereby inducing cognitive dysfunction. L-theanine can regulate the neurotransmitter content and show great potential in liver and brain protection. However, it remains unclear whether l-theanine effectively regulates neurotransmitter content under high-protein diet. A 40-day feeding experiment was performed in Sprague Dawley rats to investigate the regulatory effects and mechanisms of l-theanine on neurotransmitters via liver-brain axis in high-protein diets. The results showed that a 30% protein diet increased the liver and brain neurotransmitter content while maintaining the normal structure of liver and the hippocampal CA1 of brain and improving the autonomous behavior of rats. In contrast, 40% and 50% protein diets decreased the content of neurotransmitters, affected autonomous behavior, destroyed the hippocampal CA1 of brain structure, increased hepatic inflammatory infiltration, lipid degeneration, and hepatocyte eosinophilic change in liver, increased liver AST, ALT, MDA, CRP, and blood ammonia level, and decreased liver SOD and CAT level. However, l-theanine improved liver and brain neurotransmitter content, autonomous behavior, liver and hippocampal brain structure, and liver biochemical indicators in 40% and 50% protein diets. To explore how LTA can eliminate the adverse effects of a high-protein diet, we analyzed different metabolites and proteomes and using western blotting for validate quantitatively. We found that l-theanine regulates the activity of PF4 and G protein subunit alpha i2, increases the content of brain-derived neurotrophic factor and dopamine under a 20% protein diet. In addition, l-theanine can activate the adenylate cyclase-protein kinase A pathway through the protein alpha/beta-hydrolase domain protein 12 to regulate the content of neurotransmitters under a 40% protein diet, thereby exerting a neuroprotective effect.
作者机构：
[Wen, Shuai; Liu, Changwei; Wan, Liwei; Wang, Kuofei; Zeng, Hongzhe; Peng, Liyuan; Fang, Wenwen; Chen, Hongyu; Yang, Xiaomei] Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China;[Huang, Jian'an] Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China. Electronic address: Jian7513@hunau.edu.cn;[Liu, Zhonghua] Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China. Electronic address: zhonghua-liu-ms@hunau.edu.cn
通讯机构：
[Liu, Zhonghua; Huang, Jian'an] K;Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultural University, Changsha 410128, China. Electronic address:
摘要：
As one of the most abundant plant polyphenols in the human diet, (-)-epicatechin (EC) can improve insulin sensitivity and regulate glucose homeostasis. However, the primary mechanisms involved in EC anti-T2DM benefits remain unclear. The present study explored the effects of EC on the gut microbiota and liver transcriptome in type 2 diabetes mellitus (T2DM) Goto-Kakizaki rats for the first time. The findings showed that EC protected glucose homeostasis, alleviated systemic oxidative stress, relieved liver damage, and increased serum insulin. Further investigation showed that EC reshaped gut microbiota structure, including inhibiting the proliferation of lipopolysaccharide (LPS)-producing bacteria and reducing serum LPS. In addition, transcriptome analysis revealed that the insulin signaling pathway may be the core pathway of the EC anti-T2DM effect. Therefore, EC may modulate the gut microbiota and liver insulin signaling pathways by the gut-liver axis to alleviate T2DM. As a diet supplement, EC has promising potential in T2DM prevention and treatment.
