作者机构：
[Ya-Dan LI,; Li-Juan HU,; Gao-Shang XUE,; Hu-Hu LIU,; Hui YANG,; Yun-Hua ZHU,,; Yun TIAN,; Xiang-Yang LU,] College of Bioscience and Biotechnology,Hunan Agricultural University;[Ya-Dan LI,; Li-Juan HU,; Gao-Shang XUE,; Hu-Hu LIU,; Hui YANG,; Yun-Hua ZHU,,; Yun TIAN,; Xiang-Yang LU,] Hunan Agricultural Bioengineering Research Institute;[Ya-Dan LI,; Li-Juan HU,; Gao-Shang XUE,; Hu-Hu LIU,; Hui YANG,; Yun-Hua ZHU,,; Yun TIAN,; Xiang-Yang LU,] College of Pharmacy and Life Science,Nanhua University
会议名称：
第八届中国酶工程学术研讨会
会议时间：
2011-10-10
会议地点：
中国广东广州
会议论文集名称：
第八届中国酶工程学术研讨会论文集
关键词：
cattle;bacterial diversity;Miscanthus sinensis;restriction fragment length polymorphism
摘要：
Given the increasing recognition of the improtance of intestinal bacterial communities,a great deal of researches on the diversity of microbiota in gut of mammal have been preformed in recent years.To
摘要：
The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA. Cloning of the NtTIR1 gene will enable us to further understand the molecular organization of the TIR1 and its possible function in the tobacco.
摘要：
43 strains of cellulose-degradation fungi were isolated from the Rhizophora stylosa rhizospheric soil samples with 0-20 cm depth collected from Hongsha River of Sanya,Hainan.In order to further study
摘要：
This paper focuses on the effect of the venom of the scorpion Buthus martensii on the proliferation of human cervical carcinoma Hela cell line and the related molecular mechanism. MTT test showed that
摘要：
Cucumber (Cucumis sativus L.), which is a vegetable crop, has served as the model system for sex expression in flowering plants, and the inheritance of sex expression in cucumber is well documented. However, the genetics of subgynoecism expression in cucumber had rarely been described. In this study, we investigated the inheritance of subgynoecious traits in cucumber plants with the inbred cucumber lines of subgynoecious (C. sativus L var sativus cv 97-17 and S-2-98) as the materials. Genetic analysis had showed the two subgynoecious inbred lines were controlled by one pair of recessive gene and one pair of incompletely dominant gene, which were designated presently as mod-F2 and Mod-F1, respectively. Furthermore, the mod-F2 and Mod-F1 loci, which enhance the intensity of femaleness, also inherited independently with F and M genes. (C) 2010 Elsevier B.V. All rights reserved.
摘要：
沼气发酵系统是一个复杂的生态系统,其污泥微生物超过99%是不可培养的。为了优化沼气池纤维素的转化效率、沼气的产率和开展污泥微生物多样性研究,本研究采用化学裂解法、溶菌酶裂解法和QIAampDNA Stool Mini Kit提取了沼气池污泥样品中微生物的总DNA,对三种方法的DNA得率、纯度、大片段提取效果以及是否含有PCR反应抑制剂进行了研究,最后对16S rRNA基因V3区的扩增产物进行了PCR变性梯度凝胶电泳（PCR-DGGE）分析。与化学裂解法和QIAamp DNA Stool Mini Kit法相比,溶菌酶裂解法得到的DNA量大、片段长、片段分布广、PCR扩增效率高;同时PCR-DGGE图谱显示,溶菌酶裂解法可更好地展示沼气池污泥中微生物的多样性。该结果为进一步提高沼气池中纤维素的转化效率和沼气生产优势菌种的质和量打下了一定的前期基础。
作者机构：
[Cao L.] Key Laboratory of Chinese Materia Medica Modernization, Ministry of Education, Hunan University of Chinese Medicine, Changsha 410208, China;[卢向阳] School of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;Key Laboratory of New Drug Research and Development of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, China;[李顺祥; 黄丹] Key Laboratory of Chinese Materia Medica Modernization, Ministry of Education, Hunan University of Chinese Medicine, Changsha 410208, China, Key Laboratory of New Drug Research and Development of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha 410013, China;[魏宝阳] Key Laboratory of Chinese Materia Medica Modernization, Ministry of Education, Hunan University of Chinese Medicine, Changsha 410208, China, School of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Li, S.-X.] K;Key Laboratory of Chinese Materia Medica Modernization, Ministry of Education, Hunan University of Chinese Medicine, China
摘要：
沼气发酵系统是一个复杂的生态系统,其污泥微生物超过99%是不可培养的。为了优化沼气池纤维素的转化效率、沼气的产率和开展污泥微生物多样性研究,本研究采用化学裂解法、溶菌酶裂解法和QIAampDNA Stool Mini Kit提取了沼气池污泥样品中微生物的总DNA,对三种方法的DNA得率、纯度、大片段提取效果以及是否含有PCR反应抑制剂进行了研究,最后对16S rRNA基因V3区的扩增产物进行了PCR变性梯度凝胶电泳（PCR-DGGE）分析。与化学裂解法和QIAamp DNA Stool Mini Kit法相比,溶菌酶裂解法得到的DNA量大、片段长、片段分布广、PCR扩增效率高;同时PCR-DGGE图谱显示,溶菌酶裂解法可更好地展示沼气池污泥中微生物的多样性。该结果为进一步提高沼气池中纤维素的转化效率和沼气生产优势菌种的质和量打下了一定的前期基础。
