作者：
Xiao, Hong-Bo*;Lu, Xiang-Yang;Zhang, Heng-Bo;Sun, Zhi-Liang;Fang, Jun
期刊：
Journal of Physiology and Biochemistry,2013年69(4):719-725 ISSN：1138-7548
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Sun, Zhi-Liang] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Bioengn Res Inst, Changsha 410128, Hunan, Peoples R China.;[Zhang, Heng-Bo] Furong Dist Red Cross Hosp, Changsha 410126, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Biol Vet Drugs Branch, Changsha 410128, Hunan, Peoples R China.;[Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Undaria pinnatifida;Soluble fiber;Angiopoietin-like protein 3;Hyperlipidemia;Mice
摘要：
Angiopoietin-like protein 3 (Angptl3)-lipoprotein lipase (LPL) pathway may be a useful pharmacologic target for hyperlipidemia. The present study was conducted to test the effect of soluble fiber extracted from Undaria pinnatifida (UP), on hyperlipidemia in apolipoprotein E-deficient (ApoE(-/-)) mice. Forty mice were divided into four groups (n = 10): control group (C57BL/6J mice), ApoE(-/-) mice group, and two groups of ApoE(-/-) mice treated with UP fiber (5 or 10 % per day). UP soluble fiber treatment significantly decreased plasma and hepatic total cholesterol, triglycerides levels, plasma low-density lipoprotein cholesterol, and malondialdehyde concentrations and increased plasma high-density lipoprotein cholesterol level and downregulated protein expression of Angptl3 concomitantly with upregulated protein expression of LPL. In addition, T0901317 caused elevated expression of hepatic Angptl3 protein, and the effect of T0901317 was also abrogated by UP soluble fiber in C57BL/6J mice. The present results suggest that the UP soluble fiber regulates Angptl3-LPL pathway to lessen hyperlipidemia in mice.
摘要：
Termites are an extremely successful group of wood-degrading organisms and are therefore important both for their roles in carbon turnover in the environment and as potential sources of biochemical catalysts for efforts aimed at converting wood into biofuels. To contribute to the evolutional study of termite digestive symbiosis, a bacterial 16S rRNA gene clone library from the gut microbial community of the fungus-growing termite Macrotermes barneyi was constructed. After screening by restriction fragment length polymorphism (RFLP) analysis, 25 out of 105 clones with unique RFLP patters were sequenced and phylogenetically analyzed. Many of the clones (95%) were derived from three phyla within the domain bacteria: Bacteroidetes, Firmicutes and Proteobacteria. In addition, a few clones derived from Deferribacteres, Actinobacteria and Planctomycetes were also found. No one clone affiliated with the phylum Spirochaetes was identified, in contrast to the case of wood-feeding termites. The phylogenetic analysis revealed that nearly half of the representative clones (11 phylotypes) formed monophyletic clusters with clones obtained from other termite species, especially with the sequences retrieved from fungus-growing termites. These results indicate that the presence of termite-specific bacterial lineages implies a coevolutional relationship of gut microbes and host termites. The remaining 14 clones formed a cluster, and there was very low sequence similarity (30 to 40%) to known 16S rRNA sequences. The 16S rRNA gene sequence data showed that the majority of the intestinal microflora of M. barneyi consisted of new, uncultured species previously unknown to microbiologists.
摘要：
The aim of the present study was to establish an efficient regeneration system for the hybrid E. urophylla ×E. grandis by means of organogenesis. Stem segments from seedlings were used as explants and cultured in a modified Murashige and Skoog medium (MS), supplemented with 13.2 μM N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285 μM indole-3-acetic acid (IAA). PBU was a useful growth regulator. After cultivating for 5 d, 96% explants formed callus. After 30 d, the calli obtained were transferred to MS medium containing different combinations of 6-benzyladenine (BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage (91.3%) of the calli induced by PBU showed adventitious buds formation. Shoot elongation was then stimulated on half-strength MS mineral salts medium supplemented with 6.6 μM PBU and 0.285 μM IAA for 20 d. For rooting, the elongated shoots were cultivated on root induction medium containing 2.46 μM indole-3-butyric acid (IBA). Plantlets were then successfully transplanted to a greenhouse. This procedure represented an efficient way of E. urophylla ×E. grandis plant regeneration.
摘要：
ABA是调控植物体生长发育和响应外界应激的重要植物激素之一。近年来, ABA受体的筛选和鉴定取得了突破性进展, 为植物中ABA信号转导通路的阐明奠定了重要基础。该文主要综述了ABA-binding protein/H subunit of Mg-chelatase （ABAR/CHLH）、G protein-coupled receptor 2 （GCR2）、GPCR-type G protein 1/2 （GTG1/2）和pyrabactin resistant/PYR-like/regulatory component of ABA （PYR/PYL/RCAR）被报道为ABA受体的研究历程, 重点介绍了以ABAR/CHLH和PYR/PYL/RCAR为受体的ABA信号转导通路模型的构建, 旨在为ABA受体及其信号转导通路的相关研究提供参考。
摘要：
Ruminants harbour a complicate ecosystem consisting mostly of microorganisms which have the ability to digest the fibrous cell walls of plant materials from host's food. To discover the novel enzyme from ruminant's digestive tract for exploration of the new biofuel plant-Miscanthus sinensis, we comparatively analyzed the distribution of microbiome in the large intestine of two groups of local cattle fed with either M. sinensis [M. sinensis (+) group] or mixed forage [ M. sinensis (-) group]. Two libraries of 16S rRNA gene from intestinal microbiome of two groups of local cattle were constructed respectively, and subjected to restriction fragment length polymorphism (RFLP) and sequence analysis. After analyzing the sequences of 16S rRNA genes, our results indicated that the intestinal bacteria of M. sinensis (+) group were composed of Firmicutes (48.89%), Bacteroidetes (6.67%), rumen bacteria (10%) and uncultured bacteria (34.44%), and the intestinal bacteria of M. sinensis (-) group were composed of Firmicutes (52.87%), Bacteroidetes (1.27%), rumen bacteria (9.55%), uncultured rumen bacteria (4.46%) and uncultured bacteria (31.21%). As expected, we found that five species of potential cellulolytic becteria can only be detected in the library of M. sinensis (+). Besides, through phylogenetic tree analysis, we found that the ratio of uncultured genus which were lack of recognized sequences in M. sinensis (+) library and M. sinensis (-) library were 55.96 and 51.56%, respectively, indicating that the cattle fed on M. sinensis will produce more novel uncultured genus which probably have specific metabolic effect on decomposition of M. sinensis.
摘要：
L-arginine has been demonstrated to stimulate cell proliferation and prevent LPS-induced death of intestinal cell. To identify genes responsed to arginine treatment in intestinal cell, gene expression profiles were analyzed using high-density oligonucleotide microarray in porcine intestinal epithelial cells (IPEC-1) treated with 100 (control) or 350 μM arginine. Results showed that expression levels of 306 genes were up-regulated and 280 genes down-regulated in IPEC-1 cultured in medium containing 350 μM arginine compared with that of the control. Although the biochemical functions of some genes had not previously been defined, we were able to identify some up-regulated genes that are known to play important roles in transportion (e.g. ATP6V1G2, SLC9A4), metabolism (e.g. AKR1C4, GHRL), transcription and signal transduction (e.g. PTGFR, OCT2), signaling and proliferation (e.g. UPTI, FGFR1), cell cycle and apoptosis (e.g. DRAM1, GADD45A), cytoskeletal and cell adhesion (e.g. DNMBP, ITGB2) and immune response (e.g. OAS1, IL15), and some down-regulated genes that are related to transportion and metabolism (e.g. SLC25A25, COX1), cytoskeletal and cell adhesion (e.g. PROC, COL10A1) and immune response (e.g. SLA-DMA, CD80). The RT-PCR analysis demonstrated that 350 μM arginine treatment increased ITGB (109%), AKRIC4 (367%), ATP6VIG2 (196%), DRAMI (88%), GHRL (243%) and SLC9A2 (138%), which were almost consistent with the data obtained from the microarray analysis. Our findings provided a molecular mechanism to explain cytoprotective effects of arginine and laid the foundation for future research on cellular mechanisms of arginine regulating cell growth and proliferation.
作者：
Ouyang, L. J.;He, W. H.;Huang, Z. C.;Zhao, L. Y.;Peng, S. H.;...
期刊：
JOURNAL OF TROPICAL FOREST SCIENCE,2012年24(2):198-208 ISSN：0128-1283
通讯作者：
Zeng, F. H.
作者机构：
[Huang, Z. C.; Sha, Y. E.; Zeng, F. H.; Ouyang, L. J.; He, W. H.; Zhao, L. Y.; Peng, S. H.] Zhanjiang Normal Univ, Coll Life Sci & Technol, Zhanjiang, Guangdong, Peoples R China.;[Sha, Y. E.; Lu, X. Y.; Ouyang, L. J.; He, W. H.; Zhao, L. Y.; Peng, S. H.] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Zeng, F. H.] Z;Zhanjiang Normal Univ, Coll Life Sci & Technol, Zhanjiang, Guangdong, Peoples R China.
摘要：
We developed an Agrobacterium tumefaciens-mediated transformation system for Eucalyptus urophylla using hypocotyl explants. Antibiotic concentrations, pre-culture times, pH of the inoculation medium and co-culture times were optimised. Pre-cultured hypocotyl explants were co-cultured with A. tumefaciens strain EHA105 harbouring the binary vector pPBR-2 containing the Rs-AFP2 gene, which encodes an antifungal protein, under the control of the prp1-1 promoter, for six days and were then transferred to selective callogenesis-inducing medium containing kanamycin and cefotaxime. Calluses developed shoots and were cultured in an elongation medium and finally multiplied. The integration of T-DNA into the genome of transgenic E. urophylla was confirmed by polymerase chain reaction (PCR). The reverse transcription (RT)-PCR results showed that Rs-AFP2 gene expression could be detected only after the transformed plants were inoculated with Phytophthora capsici 60 hours after inoculation. These results indicated that the prp1-1 promoter was inducible and Rs-AFP2 could enhance the resistance of E. urophylla to P. capsici. This protocol enabled effective transformation and regeneration of E. urophylla. Kami membangunkan sistem pengubahan berantarakan Agrobacterium tumefaciens bagi Eucalyptus urophylla menggunakan eksplan hipokotil. Kepekatan antibiotik, masa prakultur, pH medium inokulasi dan masa pengkulturan bersama dioptimumkan. Eksplan hipokotil dikultur bersama-sama dengan A. tumefaciens jenis EHA105 selama enam hari dan kemudiannya dipindahkan ke medium pengaruh pengkalusan terpilih yang mengandungi kanamisin dan sefotaksim. Baka ini menyimpan vektor perduaan pPBR-2 yang mengandungi gen Rs-AFP2 yang mengekod protein antikulat di bawah kawalan penggalak prp1-1. Kalus menghasilkan pucuk dan dikultur dalam medium pemanjangan dan kemudiannya digandakan. Integrasi T-DNA ke dalam genom E. urophylla yang transgenik disahkan oleh reaksi rantai polimerase (PCR). Keputusan trankripsi (RT)-PCR yang bertentangan menunjukkan yang gen Rs-AFP2 terperi boleh dikesan hanya selepas tumbuhan terubah telah diinokulasi dengan Phytophthora capsici (60 jam selepas inokulasi). Keputusan menunjukkan yang penggalak prp1-1 boleh diaruh dan Rs-AFP2 boleh meningkatkan kerintangan E. urophylla terhadap P. capsici. Protokol ini membolehkan pengubahan dan pertumbuhan semula E. urophylla yang berkesan.
