通讯机构：
[Li, YC; Deng, QY] H;Hunan Rice Res Inst, 732 Yuanda Rd, Changsha 410125, Hunan, Peoples R China.
关键词：
Cadmium (Cd) stress;high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS);isobaric tag for relative and absolute quantitation (iTRAQ);proteomics;rice
通讯机构：
[Lu, XY; Qin, S] H;[Qin, Si] K;Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Core Res Program 1515, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;Kagoshima Univ, Fac Agr, United Grad Sch Agr Sci, Korimoto 1-21-24, Kagoshima 8900065, Japan.
摘要：
Myricetin has been reported as a promising chemopreventive compound with multiple biofunctions. To evaluate its influence on gene expressions in genome-wide set and further investigate its anti-inflammatory property, the present study performed Gene Ontology and Ingenuity Pathway Analysis (IPA) to describe the basic gene expression characteristics by myricetin treatment in HepG2 cells, confirmed its multi-biofunction by real-time fluorescent quantitative PCR (RT-qPCR), and further verified its anti-inflammatory property by Western blotting and bio-plex-based cytokines assay. The IPA data showed that 337 gene expressions (48% of the top molecules) are disturbed over 2-fold, and the most possible biofunctions of myricetin are the effect on "cardiovascular disease, metabolic disease, and lipid metabolism," via regulation of 28 molecules with statistic score of 46. RT-qPCR data confirmed the accuracy of microarray data, and cytokines assay results indicated that 6 of the total 27 inflammatory cytokine secretions were significantly inhibited by myricetin pretreatment, including TNF-alpha, IFN-gamma, IL-1alpha, IL-1beta, IL-2, and IL-6. The present study is the first time to elucidate the multi-function of myricetin in genome-wide set by IPA analysis and verify its anti-inflammatory property by proteomics of cytokines assay. Therefore, these results enrich the comprehensive bioactivities of myricetin and reveal that myricetin has powerful anti-inflammatory property, which provides encouragement for in vivo studies to verify its possible health benefits.
摘要：
In this paper, a series of novel orange-red emission phosphors Sr8ZnY(PO4)(7):Sm3+ (SZYP:Sm3+) were synthesized by high-temperature solid-state reaction. The photoluminescence and concentration-dependent properties of this phosphor were investigated carefully through diffuse reflection spectra, excitation and emission spectra, Commission Internationale de l'Eclairage (CIE) chromaticity coordinate and decay times. SZYP:Sm3+ could be efficiently excited by n-UV and blue lights which center at 318, 345, 363, 376, 401, 410, 440 and 475 nm, respectively. The characteristic orange-red emission peaking at 563, 601 and 647 nm had been detected, which should be attributed to the characteristic f-f forbidden transition of Sm3+. The internal quantum efficiency (IQE) of ideal phosphor of SZYP:0.09Sm(3+) reached as high as 47%. In addition, The concentration quenching mechanism and thermal stability of SZYP:Sm3+ were further investigated. It was found SZYP:Sm3+ can maintain 93% of initial emission intensity at 150 degrees C and the corresponding activation energy was calculated to be 0.26 eV based on Arrhenius formula. The emission intensity of 1.0Mg(2+) and 0.02Al(3+) incorporated SZYP:0.09Sm(3+) reached up to 134% and 117% of the initial value, respectively. Moreover, (light-emitting diodes) LED devices were fabricated by employing the optimized samples as orange-red component with 365 nm n-UV chips. The desired emission in plant absorption region and extremely high thermal stability imply that SZYP:Sm3+ is suitable for the application in plant growth LED lighting. (C) 2019 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
作者机构：
[Wu, An-Jun; Ma, Qiang; Wu, Xiong-Wei; Peng, Chang; Deng, Q; Lu, Xiang-Yang; Deng, Qi; Zeng, Xian-Xiang; Zhou, Chun-Jiao] Hunan Agr Univ, Coll Sci, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yin, Ya-Xia; Guo, Yu-Guo] Chinese Acad Sci, Inst Chem, CAS Key Lab Mol Nanostruct & Nanotechnol, CAS Res Educ Ctr Excellence Mol Sci,BNLMS, Beijing 100190, Peoples R China.;[Yin, Ya-Xia; Guo, Yu-Guo] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
通讯机构：
[Deng, Q; Lu, XY] H;[Guo, Yu-Guo] C;[Guo, Yu-Guo] U;Hunan Agr Univ, Coll Sci, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Inst Chem, CAS Key Lab Mol Nanostruct & Nanotechnol, CAS Res Educ Ctr Excellence Mol Sci,BNLMS, Beijing 100190, Peoples R China.
关键词：
vanadium redox flow batteries;electrocatalysis;carbon fibers;heteroatoms gradient distribution;long service life
摘要：
The fundamental understanding of electrocatalytic reaction process is anticipated to guide electrode upgradation and acquirement of high-performance vanadium redox flow batteries (VRFBs). Herein, a carbon fiber prototype system with a heteroatom gradient distribution has been developed with enlarged interlayer spacing and a high graphitization that improve the electronic conductivity and accelerate the electrocatalytic reaction, and the mechanism by which gradient-distributed heteroatoms enhance vanadium redox reactions was elucidated with the assistance of density functional theory calculations. All these contributions endow the obtained electrode prominent redox reversibility and durability with only 1.7% decay in energy efficiency over 1000 cycles at 150 mA cm(-2) in the VRFBs. Our work sheds light on the significance of elaborated electrode design and impels the in-depth investigation of VRFBs with long service life.
摘要：
As a new class of promising fluorescent carbon nanomaterials, carbon dots (CDs) have been well developed in recent years for their excellent fluorescent properties, simple synthetic approaches, good biocompatibility and various detection applications, which can be expected to replace the tradition semi-quantum dots. This review aims presents the current progress in the development of CDs with an emphasis on fluorescent properties, synthetic approaches and applications in food safety. First, the fluorescent properties of CDs are briefly introduced. To seek more reasonable synthetic approaches, the characteristics of the diverse methods of CD synthesis are summarized. And then, applications of CDs as fluorescent probes in food safety are discussed, with emphasis on the determination of metal ions/anions, pesticides, veterinary drugs, bacteria, functional components and banned additives. Finally, the challenges, such as how to explain the diversity of fluorescent properties, and how to form a uniform synthesis procedure to improve the quantum yield (QY), for CDs are presented. Although CDs have found many applications in food safety, it is still a challenge to detect a specific target in complex samples. Therefore, combination with other biochemistry technology to exploit novel ligands against the specific target with high binding affinity and selectivity is vitally important for developing sensitive and specific sensing probes in the future.
摘要：
The pollen development of male-sterile lines should be conducted during the high temperature season to ensure the sterility of male-sterile line pollen and the safety of hybrid seed production in the breeding of thermosensitive genic male-sterile (TGMS) lines of early rice. However, the mature hybrid rice seeds are prone to pre-harvest sprouting (PHS) during hot, rainy days, which leads to the decrease of hybrid rice seed quality or a loss of utilization value. In this study, with an aim to bring a dormancy trait from the dormant genotype to the current low-dormant TGMS lines, a common wild rice “Chaling” (Oryza rufipogon), which has strong seed dormancy, a large black stigma, and a high exsertion rate, was hybridized with the early rice TGMS line “Zhu 1S,” which often exhibits PHS. After screening for early maturing and seed dormancy, the early rice “Yezao S” and “Yezhuzao S,” with the characteristic of seed dormancy, were selected. The critical temperature of high temperature sterility for these two male-sterile lines was below 23.5 °C. The stigma of “Yezao S” was black and its exsertion rate was 79.9%, while “Yezhuzao S” had a colorless stigma with an exsertion rate of 76.8%. The expression of the genes OsNCEDs in “Yezao S” and “Yezhuzao S” were significantly higher than that in “Zhu 1S” the 15th day after heading, and the abscisic acid (ABA) content was also higher than that of “Zhu 1S” the 20th day after heading. The results indicated that introducing the dormancy characteristics from wild rice “Chaling” into the early rice TGMS lines was an effective way to prevent PHS.
期刊：
New Phytologist,2018年217(1):332-343 ISSN：0028-646X
通讯作者：
Huang, Rongfeng;Zhang, Haiwen
作者机构：
[Xiao, Guiqing; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Guiqing; Huang, RF; Zhang, Haiwen; Huang, Rongfeng; Zhou, Jiahao] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.
通讯机构：
[Huang, RF; Zhang, HW] C;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.
关键词：
UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1);caspase-like activity;lesion;programmed cell death (PCD);reactive oxygen species (ROS);rice (Oryza sativa);uridine 5'-diphosphoglucoseglucose (UDPG)
摘要：
<jats:title>Summary</jats:title><jats:p>
<jats:list list-type="bullet">
<jats:list-item><jats:p>Lesion mimic mutants are valuable to unravel the mechanisms governing the programmed cell death (PCD) process. Uridine 5′‐diphosphoglucose‐glucose (UDPG) functions as a signaling molecule activating multiple pathways in animals, but little is known about its function in plants.</jats:p></jats:list-item>
<jats:list-item><jats:p>Two novel allelic mutants of <jats:italic>spl29</jats:italic> with typical PCD characters and reduced pollen viability were obtained by ethane methyl sulfonate mutagenesis in rice cv <jats:italic>Kitaake</jats:italic>.</jats:p></jats:list-item>
<jats:list-item><jats:p>The enzymatic analyses showed that UDP‐N‐acetylglucosamine pyrophosphorylase 1 (UAP1) irreversibly catalyzed the decomposition of UDPG. Its activity was severely destroyed and caused excessive UDPG accumulation, with the lesion occurrence associated with the enhanced caspase‐like activities in <jats:italic>spl29‐2</jats:italic>. At the transcriptional level, several key genes involved in endoplasmic reticulum stress and the unfolded protein response were abnormally expressed. Moreover, exogenous UDPG could aggravate lesion initiation and development in <jats:italic>spl29‐2</jats:italic>. Importantly, exogenous UDPG and its derivative UDP‐N‐acetylglucosamine could induce reactive oxygen species (ROS) accumulation and lesion mimics in <jats:italic>Kitaake</jats:italic> seedlings.</jats:p></jats:list-item>
<jats:list-item><jats:p>These results suggest that the excessive accumulation of UDPG, caused by the mutation of <jats:italic>UAP1</jats:italic>, was a key biochemical event resulting in the lesion mimics in <jats:italic>spl29‐2</jats:italic>. Thus, our findings revealed that UDPG might be an important component involved in ROS accumulation, PCD execution and lesion mimicking in rice, which also provided new clues for investigating the connection between sugar metabolism and PCD process.</jats:p></jats:list-item>
</jats:list>
</jats:p>
摘要：
P2X receptors are a family of extracellular ATP-gated trimeric cation channels that is widely distributed in human tissues. Quite some drug candidates targeting P2X receptors have entered into preclinical or main phases of clinical trials, but many of them failed due to low subtype-selectivity or species differences in pharmacological activities between human and experimental animals. Here, we identified the distinct inhibitory efficacies of NF110, a competitive inhibitor, between the rat (rP2X3) and human (hP2X3) P2X3 receptors. We demonstrated that this difference is determined by two amino acids located in the dorsal fin (DF) domain of P2X3 receptors. As revealed by mutagenesis, metadynamics, and covalent modification, NF110-mediated rP2X3 inhibition may be through a filling in the cavity formed by the DF, left flipper (LF) and lower body (LB) to partially, rather than fully, occupy the ATP-binding pocket. Moreover, substitution of residues located in the DF and/or LF domains of the rP2X2 receptor, a NF110-insensitive subtype, with the equivalent amino acids of rP2X3, bestowed the sensitivity of rP2X2 to NF110. The critical roles of the DF and LF domains in channel gating of P2X and low conservativity in residue sequences of those two domains raise the possibility that small molecules differentially interacting with the residues of the DF and LF domains of different P2X receptors may modulate channel's activity in a subtype-selective manner. However, the possible species-specificity of P2X inhibitors/modulators makes it more complex when interpreting the preclinical data into clinical researches. Nevertheless, our data provide new insights into the subtype-selectivity of competitive inhibitors and their distinct potencies in the human and experimental animals, both of which are extremely important in the drug discovery of P2X receptors. (C) 2018 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.
