作者： Liu, Sanxiong;Li, Yongchao*;Liu, Licheng;Min, Jun;Liu, Wenqiang;...

期刊： Analytical Letters,2020年53(5):807-820 ISSN：0003-2719

通讯作者： Li, Yongchao;Deng, Qiyun

作者机构： [Li, Yongchao; Pan, Xiaowu; Min, Jun; Liu, Sanxiong; Li, Xiaoxiang; Liu, Licheng; Liu, Wenqiang] Hunan Acad Agr Sci, Hunan Rice Res Inst, Changsha, Hunan, Peoples R China.;[Li, Yongchao; Pan, Xiaowu; Min, Jun; Liu, Sanxiong; Li, Xiaoxiang; Liu, Licheng; Liu, Wenqiang] Minist Agr, Key Lab Ind Rice Genet & Breeding Middle & Lower, Changsha, Hunan, Peoples R China.;[Deng, Qiyun; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Deng, Qiyun] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China.;[Li, YC; Deng, QY] Hunan Rice Res Inst, 732 Yuanda Rd, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Li, YC; Deng, QY] H;Hunan Rice Res Inst, 732 Yuanda Rd, Changsha 410125, Hunan, Peoples R China.

关键词： Cadmium (Cd) stress;high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS);isobaric tag for relative and absolute quantitation (iTRAQ);proteomics;rice

摘要： Cadmium (Cd) is a heavy metal used in industrial processes that impedes the growth of plants. Although intensive work has concentrated on the mechanism of rice under Cd stress, few studies compared the proteomic information on the mechanism between different cultivars. Different rice cultivars were screened and two cultivars, the japonica cultivar Bancanggengnuo (BCGN) and indica cultivar Yuzhenxiang (YZX) were selected to be the varieties for proteomic experiments. High-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) with an isobaric tag for relative and absolute quantitation (iTRAQ) was used to analyze the global protein profiles of these two cultivars. There were 109 and 209 differentially expressed proteins (DEPs) in the Cd treatment groups for BCGN and YZX respectively and 61 additional DEPs between cultivars after treatment. Pathway enrichment analysis showed that three pathways were altered in the cultivars, which affected photosynthesis and redox reactions. A total of 37 up- and 27 down-regulated proteins were identified in both cultivars exposed to Cd. The molecular regulatory network revealed that several proteins associated with carbohydrate metabolism, redox reaction and signal transduction were involved in and acted as the intersection point of disturbed metabolism. This is the first report on the comprehensive proteomic characterization of Cd-exposed leaf proteomes in rice. These findings may facilitate the unraveling molecular mechanism underlying Cd stress response in rice, and provide fundamental insights into the development of Cd-resistant rice cultivars. This work has explored the mechanism of Cd resistance at the protein level, which may be a novel target for improving the resistance of rice to this heavy metal. © 2019, © 2019 Taylor & Francis Group, LLC.

语种： 英文

作者： 徐圣尧;李彬;卢向阳;田云

期刊： 生命科学,2019年31(8):812-818 ISSN：1004-0374

作者机构： 湖南农业大学生物科学技术学院,长沙,410128;[田云; 李彬; 卢向阳; 徐圣尧] 湖南农业大学

关键词： P2X3受体;激活剂;抑制剂

摘要： P2X受体是由胞外ATP激活的配体型门控阳离子通道,其亚型P2X3受体在咳嗽、疼痛、高血压等疾病的发生过程中发挥重要作用,以P2X3受体为药物靶标开展研究对疾病的治疗有重要意义。该文就P2X3受体的结构、激活剂及抑制剂的作用机理展开综述。

语种： 中文

作者： Zhou, Nan;Gao, Peixing;Yang, Yimei;Zhong, Yuan*;Xia, Mao;...

期刊： Journal of the Taiwan Institute of Chemical Engineers,2019年104:360-368 ISSN：1876-1070

通讯作者： Zhong, Yuan;Zhou, Zhi

作者机构： [Xia, Mao; Zhong, Yuan; Zhou, Zhi; Zhang, Ye; Yang, Yimei; Zhou, Nan; Gao, Peixing] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Xia, Mao; Zhang, Ye; Yang, Yimei; Zhong, Yuan; Zhou, Nan; Gao, Peixing; Zhou, Zhi] Hunan Prov Engn Technol Res Ctr Opt Agr, Changsha 410128, Hunan, Peoples R China.;[Tian, Yun; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Zhong, Y; Zhou, Z] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.

关键词： Phosphors;Sm3+-activated;Thermal stability;Plant growth lighting

摘要： In this paper, a series of novel orange-red emission phosphors Sr8ZnY(PO4)(7):Sm3+ (SZYP:Sm3+) were synthesized by high-temperature solid-state reaction. The photoluminescence and concentration-dependent properties of this phosphor were investigated carefully through diffuse reflection spectra, excitation and emission spectra, Commission Internationale de l'Eclairage (CIE) chromaticity coordinate and decay times. SZYP:Sm3+ could be efficiently excited by n-UV and blue lights which center at 318, 345, 363, 376, 401, 410, 440 and 475 nm, respectively. The characteristic orange-red emission peaking at 563, 601 and 647 nm had been detected, which should be attributed to the characteristic f-f forbidden transition of Sm3+. The internal quantum efficiency (IQE) of ideal phosphor of SZYP:0.09Sm(3+) reached as high as 47%. In addition, The concentration quenching mechanism and thermal stability of SZYP:Sm3+ were further investigated. It was found SZYP:Sm3+ can maintain 93% of initial emission intensity at 150 degrees C and the corresponding activation energy was calculated to be 0.26 eV based on Arrhenius formula. The emission intensity of 1.0Mg(2+) and 0.02Al(3+) incorporated SZYP:0.09Sm(3+) reached up to 134% and 117% of the initial value, respectively. Moreover, (light-emitting diodes) LED devices were fabricated by employing the optimized samples as orange-red component with 365 nm n-UV chips. The desired emission in plant absorption region and extremely high thermal stability imply that SZYP:Sm3+ is suitable for the application in plant growth LED lighting. (C) 2019 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

语种： 英文

作者： Li, Tao;Zhu, Jihe;Deng, Fangming;Wu, Weiguo;Zheng, Zhibing;...

期刊： BioMed Research International,2019年2019:3746326 ISSN：2314-6133

通讯作者： Lu, Xiangyang;Qin, Si

作者机构： [Lu, XY; Qin, S; Li, Tao; Deng, Fangming; Wu, Weiguo; Zheng, Zhibing; Lu, Xiangyang; Lv, Chenghao; Qin, Si; Li, Yong; Zhu, Jihe] Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Core Res Program 1515, Changsha 410128, Hunan, Peoples R China.;[Xiang, Wei; Lu, XY; Qin, S; Lu, Xiangyang; Qin, Si] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Qin, Si] Kagoshima Univ, Fac Agr, United Grad Sch Agr Sci, Korimoto 1-21-24, Kagoshima 8900065, Japan.

通讯机构： [Lu, XY; Qin, S] H;[Qin, Si] K;Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Core Res Program 1515, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;Kagoshima Univ, Fac Agr, United Grad Sch Agr Sci, Korimoto 1-21-24, Kagoshima 8900065, Japan.

摘要： Myricetin has been reported as a promising chemopreventive compound with multiple biofunctions. To evaluate its influence on gene expressions in genome-wide set and further investigate its anti-inflammatory property, the present study performed Gene Ontology and Ingenuity Pathway Analysis (IPA) to describe the basic gene expression characteristics by myricetin treatment in HepG2 cells, confirmed its multi-biofunction by real-time fluorescent quantitative PCR (RT-qPCR), and further verified its anti-inflammatory property by Western blotting and bio-plex-based cytokines assay. The IPA data showed that 337 gene expressions (48% of the top molecules) are disturbed over 2-fold, and the most possible biofunctions of myricetin are the effect on "cardiovascular disease, metabolic disease, and lipid metabolism," via regulation of 28 molecules with statistic score of 46. RT-qPCR data confirmed the accuracy of microarray data, and cytokines assay results indicated that 6 of the total 27 inflammatory cytokine secretions were significantly inhibited by myricetin pretreatment, including TNF-α, IFN-γ, IL-1α, IL-1β, IL-2, and IL-6. The present study is the first time to elucidate the multi-function of myricetin in genome-wide set by IPA analysis and verify its anti-inflammatory property by proteomics of cytokines assay. Therefore, these results enrich the comprehensive bioactivities of myricetin and reveal that myricetin has powerful anti-inflammatory property, which provides encouragement for in vivo studies to verify its possible health benefits. © 2019 Tao Li et al.

语种： 英文

作者： Wu, Xiong-Wei;Deng, Qi*;Peng, Chang;Zeng, Xian-Xiang;Wu, An-Jun;...

期刊： ACS Applied Materials & Interfaces,2019年11(12):11451-11458 ISSN：1944-8244

通讯作者： Deng, Qi;Lu, Xiang-Yang;Guo, Yu-Guo

作者机构： [Wu, An-Jun; Ma, Qiang; Wu, Xiong-Wei; Peng, Chang; Deng, Q; Lu, Xiang-Yang; Deng, Qi; Zeng, Xian-Xiang; Zhou, Chun-Jiao] Hunan Agr Univ, Coll Sci, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yin, Ya-Xia; Guo, Yu-Guo] Chinese Acad Sci, Inst Chem, CAS Key Lab Mol Nanostruct & Nanotechnol, CAS Res Educ Ctr Excellence Mol Sci,BNLMS, Beijing 100190, Peoples R China.;[Yin, Ya-Xia; Guo, Yu-Guo] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.

通讯机构： [Deng, Q; Lu, XY] H;[Guo, Yu-Guo] C;[Guo, Yu-Guo] U;Hunan Agr Univ, Coll Sci, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Inst Chem, CAS Key Lab Mol Nanostruct & Nanotechnol, CAS Res Educ Ctr Excellence Mol Sci,BNLMS, Beijing 100190, Peoples R China.

关键词： vanadium redox flow batteries;electrocatalysis;carbon fibers;heteroatoms gradient distribution;long service life

摘要： The fundamental understanding of electrocatalytic reaction process is anticipated to guide electrode upgradation and acquirement of high-performance vanadium redox flow batteries (VRFBs). Herein, a carbon fiber prototype system with a heteroatom gradient distribution has been developed with enlarged interlayer spacing and a high graphitization that improve the electronic conductivity and accelerate the electrocatalytic reaction, and the mechanism by which gradient-distributed heteroatoms enhance vanadium redox reactions was elucidated with the assistance of density functional theory calculations. All these contributions endow the obtained electrode prominent redox reversibility and durability with only 1.7% decay in energy efficiency over 1000 cycles at 150 mA cm -2 in the VRFBs. Our work sheds light on the significance of elaborated electrode design and impels the in-depth investigation of VRFBs with long service life. © 2019 American Chemical Society.

语种： 英文

作者： Liu, Hu-Hu;Wang, Chong;Lu, Xiang-Yang;Huang, He;Tian, Yun*;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019年67(35):9851-9857 ISSN：0021-8561

通讯作者： Tian, Yun;Ji, Xiao-Jun

作者机构： [Tian, Yun; Liu, Hu-Hu; Huang, He; Wang, Chong; Lu, Xiang-Yang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Huang, He; Ji, Xiao-Jun] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China.

通讯机构： [Tian, Yun] H;[Ji, Xiao-Jun] N;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, 30 South Puzhu Rd, Nanjing 211816, Jiangsu, Peoples R China.

关键词： arachidonic acid;Yarrowia lipolytica;pathway engineering;protein engineering

摘要： Arachidonic acid (ARA, C20:4) is a typical ω-6 polyunsaturated fatty acid with special functions. Using Yarrowia lipolytica as an unconventional chassis, we previously showed the performance of the Î"-6 pathway in ARA production. However, a significant increase in the Î"-9 pathway has rarely been reported. Herein, the Î"-9 pathway from Isochrysis galbana was constructed via pathway engineering, allowing us to synthesize ARA at 91.5 mg L-1. To further improve the ARA titer, novel enzyme fusions of Î"-9 elongase and Î"-8 desaturase were redesigned in special combinations containing different linkers. Finally, with the integrated pathway engineering and synthetic enzyme fusion, a 29% increase in the ARA titer, up to 118.1 mg/L, was achieved using the reconstructed strain RH-4 that harbors the rigid linker (GGGGS). The results show that the combined pathway and protein engineering can significantly facilitate applications of Y. lipolytica. © 2019 American Chemical Society.

语种： 英文

作者： 曾子毅;丁诗瑶;卢向阳;姚慧;王蓉蓉;...

期刊： 中国酿造,2019年38(1):75-79 ISSN：0254-5071

作者机构： 湖南农业大学食品科学技术学院湖南省食品科学与生物技术重点实验室,湖南长沙410128;[卢向阳] 瑚南农业大学生物科学技术学院,湖南长沙410128;湖南南山牧业有限公司,湖南城步422512;[肖茜; 曾子毅; 周辉; 刘成国; 王蓉蓉; 丁诗瑶] 湖南农业大学;[姚慧] 湖南南山牧业有限公司

关键词： 植物乳杆菌;β-葡萄糖苷酶;特性

摘要： 通过比色法测定酶活,比较5株植物乳杆菌(Lactobacillus plantarum)产β-葡萄糖苷酶的特性,对胞内β-葡萄糖苷酶最佳反应pH和温度进行了研究,考察了氯化钠及乙醇对β-葡萄糖苷酶活性的影响.结果 表明,5株植物乳杆菌都可以产生胞内p-葡萄糖苷酶,其中比酶活最高的为菌株W-4 (14.168 U/g),其次是菌株Y-12(13.015 U/g),之后分别是菌株Y-20 (11.359 U/g)、菌株1.3919 (7.029 U/g)及菌株Y-22 (4.630 U/g);植物乳杆菌W-4和1.3919还可以产生胞外酶;菌株W-4的胞内β-葡萄糖苷酶的最适反应pH为4.0～4.5,菌株Y-12的最适pH为4.5,菌株Y-20和1.3919的最适pH为5.5,菌株Y-22的最适pH为5.0;菌株W-4、Y-20、Y-22及1.3919的最适反应温度均为30℃,而菌株Y-12的最适反应温度为37℃.不同含量的氯化钠和乙醇对5株植物乳杆菌的β-葡萄糖苷酶活性具有一定的抑制作用,且浓度与抑制作用呈正相关.

语种： 中文

作者： 周赓;陈宸;刘晓虹;卢向阳;田云;...

期刊： 植物生理与分子生物学学报,2019年55(7):902-914 ISSN：1671-3877

通讯作者： Tian, Y.

作者机构： 湖南大学研究生院隆平分院,长沙410125;湖南省农业科学院,湖南省蔬菜研究所,长沙410125;湖南农业大学生物科学技术学院,长沙,410125

通讯机构： College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China

关键词： 黄瓜;性别决定;性别决定基因表达;激素调控

摘要： 黄瓜(Cucumis sativus)是进行性别研究的主要模式植物之一。性别决定机制的研究对于提高黄瓜雌花结实的产量具有重要意义。研究表明黄瓜植株的性别主要受黄瓜性别决定基因、环境条件和植物激素的影响。本文综述了近年来黄瓜性别决定的研究进展,并对黄瓜单性花发育机制以及黄瓜性别决定基因的时空表达提出了展望。

语种： 中文

作者： Shi, Xingbo*;Wei, Wei;Fu, Zhaodi;Gao, Wenli;Zhang, Chunyan;...

期刊： Talanta,2019年194:809-821 ISSN：0039-9140

通讯作者： Shi, Xingbo;Deng, Fangming;Lu, Xiangyang

作者机构： [Shi, Xingbo; Gao, Wenli; Zhang, Chunyan; Shi, XB; Dene, FM; Deng, Fangming; Zhao, Qian; Wei, Wei] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Shi, Xingbo; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Fu, Zhaodi] Changsha Res Inst Min & Met CO LTD, Analyt Testing Lab, Changsha 410012, Hunan, Peoples R China.

通讯机构： [Shi, XB; Dene, FM; Lu, Xiangyang] H;Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

关键词： Carbon dots;Fluorescent properties;Food safety;Sensors

摘要： As a new class of promising fluorescent carbon nanomaterials, carbon dots (CDs) have been well developed in recent years for their excellent fluorescent properties, simple synthetic approaches, good biocompatibility and various detection applications, which can be expected to replace the tradition semi-quantum dots. This review aims presents the current progress in the development of CDs with an emphasis on fluorescent properties, synthetic approaches and applications in food safety. First, the fluorescent properties of CDs are briefly introduced. To seek more reasonable synthetic approaches, the characteristics of the diverse methods of CD synthesis are summarized. And then, applications of CDs as fluorescent probes in food safety are discussed, with emphasis on the determination of metal ions/anions, pesticides, veterinary drugs, bacteria, functional components and banned additives. Finally, the challenges, such as how to explain the diversity of fluorescent properties, and how to form a uniform synthesis procedure to improve the quantum yield (QY), for CDs are presented. Although CDs have found many applications in food safety, it is still a challenge to detect a specific target in complex samples. Therefore, combination with other biochemistry technology to exploit novel ligands against the specific target with high binding affinity and selectivity is vitally important for developing sensitive and specific sensing probes in the future.

语种： 英文

作者： 潘虎;刘青海;田云;达娃卓玛;卢向阳;...

期刊： 河南农业科学,2019年48(04):59-64 ISSN：1004-3268

作者机构： [潘虎] 西藏自治区农牧科学院 农业质量标准与检测研究所,西藏 拉萨 850032;[潘虎] 湖南农业大学 生物科学技术学院,湖南 长沙 410128;西藏自治区农牧科学院 农业质量标准与检测研究所,西藏 拉萨,850032;湖南农业大学 生物科学技术学院,湖南 长沙,410128;[田云; 卢向阳] 湖南农业大学

关键词： 拉萨;青稞;农田土壤;真菌区系

摘要： 为明确拉萨青稞种植农田土壤中真菌群落区系组成,采用Illumina高通量测序技术对拉萨9处不同青稞种植农田土壤进行了真菌群落结构解析.结果表明,随着青稞的生长发育,其土壤样品中真菌丰富度和多样性逐渐增高,土壤真菌群落在门的水平上主要为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、壶菌门(Chytridiomycota)、球囊菌门(Glomeromycota)和接合菌门(Zygomyco-ta),在属的水平上主要为被孢霉属(Mortierella)、足孢子菌属(Podospora)、赤霉菌属(Gibberella)、分子孢子菌属(Cladosporium)和毛壳菌属(Chaetomium)等.青稞灌浆期较播种前期、收获后期真菌群落组成差异较小,其土壤真菌群落具有趋同特征.

语种： 中文

作者： Luo, Luyun;Zhang, Zhuo;Wang, Pei;Han, Yongqin;Jin, Decai;...

期刊： AMB EXPRESS,2019年9(1):1-13 ISSN：2191-0855

通讯作者： Lu, Xiangyang;Liu, Yong

作者机构： [Tan, Xinqiu; Luo, Luyun; Zhang, Deyong; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Luo, Luyun; Han, Yongqin; Su, Pin; Zhang, Zhuo; Muhammad-Rizwan, Hamid; Liu, Yong; Wang, Pei] Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Pest Management Hort Crops Hunan Prov, Changsha, Hunan, Peoples R China.;[Jin, Decai] Chinese Acad Sci, Key Lab Environm Biotechnol, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China.

通讯机构： [Lu, Xiangyang; Liu, Yong] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;Hunan Acad Agr Sci, Hunan Plant Protect Inst, Key Lab Pest Management Hort Crops Hunan Prov, Changsha, Hunan, Peoples R China.

关键词： Phyllosphere;Microbial community;Lesion coverage rate;α Diversity;Plant-specific microbe

摘要： The phyllosphere is colonized by a wide variety of microorganisms including epiphytes, plant-pathogenic fungus, bacteria, as well as human or animal pathogens. However, little is known about how microbial community composition changes with the development of angular leaf-spot of cucumber. Here, 18 mixed samples were collected based on the lesion coverage rate (LCR) of angular leaf-spot of cucumber from three disease severity groups (DM1: symptomatic-mild, DM2: symptomatic-moderate, DM3: symptomatic-severe). In our study, the microbial community structure and diversity were examined by Illumina MiSeq sequencing. A significant differences was observed in α diversity and community structure among three disease severity groups. The phyllosphere microbiota was observed to be dominated by bacterial populations from Proteobacteria, Actinobacteria, and Firmicutes, as well as fungal species from Ascomycota and Basidiomycota. In addition, some plant-specific microbe such as Sphingomonas, Methylobacterium, Pseudomonas, and Alternaria showed significant changes in their relative abundance of population. The LCR was correlated negatively with Sphingomonas, Methylobacterium, Quadrisphaera, and Lactobacillus, whereas correlated positively with Pseudomonas and Kineococcus (p < 0.05). The LCR was negatively correlated with Alternaria and Arthrinium of the fungal communities (p < 0.05). Molecular ecological networks of the microbial communities were constructed to show the interactions among the OTUs. Our current results indicated that the competitive relationships among species were broken with the development of angular leaf-spot of cucumber. The microbial community composition changed over the development of angular leaf-spot of cucumber. The result of molecular ecological networks indicated that the overall bacterial community tends toward mutualism from the competition. The development of angular leaf-spot of cucumber affected the ecosystem functioning by disrupting the stability of the microbial community network. This work will help us to understand the host plant-specific microbial community structures and shows how these communities change throughout the development of angular leaf-spot of cucumber. © 2019, The Author(s).

语种： 英文

作者： 杨辉;杨晓娜;王翀;卢向阳;田云

期刊： 湖南农业大学学报(自然科学版),2018年44(3):271-277 ISSN：1007-1032

作者机构： [杨辉; 杨晓娜; 王翀; 卢向阳; 田云] 湖南农业大学生物科学技术学院;[杨辉; 杨晓娜; 王翀; 卢向阳; 田云] 农业生物化学与生物转化湖南省高校重点实验室;[杨辉; 杨晓娜; 王翀; 卢向阳; 田云] 畜禽废弃物资源化利用湖南省工程实验室

关键词： 烟草;转录激活;基因表达

摘要： 以烟草品种NC89为试验材料,通过电子克隆获得1个烟草GRAS家族基因NtGRAS。该基因编码区长为1 311 bp,编码436个氨基酸。生物信息学分析结果显示,NtGRAS蛋白的相对分子质量为47 781,等电点为5.16,亚细胞定位于细胞核。进化树分析发现,NtGRAS蛋白与拟南芥AtSCL23蛋白高度同源。酵母转录激活结果表明,NtGRAS蛋白具有转录激活活性。实时荧光定量PCR分析表明,NtGRAS在烟草的根、茎、叶和花中均有表达,其中在根部的表达量最高;低温(4 ℃)、NaCl (200 μmol/L)和干旱均能诱导NtGRAS基因上调表达。

语种： 中文

作者： Li, Hui-yong;Yao, Deng-hui;Feng, Qiu-ju;Zeng, Hai-bo;Liang, Jian-mei;...

期刊： Desalination and Water Treatment,2018年118:195-204 ISSN：1944-3994

通讯作者： Zhou, Nan;Lu, Xiang-yang

作者机构： [Li, Hui-yong; Zeng, Hai-bo; Liang, Jian-mei; Yao, Deng-hui; Zhou, Zhi; Zhou, Nan] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Feng, Qiu-ju] Jishou Univ, Coll Chem & Chem Engn, Jishou 416000, Peoples R China.;[Tian, Yun; Zhou, Nan; Lu, Xiang-yang; Zhou, N] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Zhou, Nan; Zhou, N; Lu, XY] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

关键词： Biochar;Grape vine shoots;Heavy metals removal;Minerals;Precipitation;Pyrolysis temperature

摘要： Grape vine shoots were utilized as the biomass feedstock and pyrolyzed under temperatures ranging in 300°C–600°C with nitrogen atmosphere to produce biochars. The surface properties of the as-obtained biochars were characterized, followed with the careful investigation of the adsorption of lead and cadmium removal in aqueous environments. All as-prepared biochars had weak alkali characteristics and large amount of mineral species, which can greatly improve the adsorption performance. Outstanding storing capacities of 250.81, 327.31, 377.36 mg g –1 for Cd 2+ and 156.12, 237.25, 258.86 mg g –1 for Pb 2+ were exhibited by biochar pyrolyzed under 300°C, 450°C and 600°C, respectively. The adsorption equilibrium could be reached about 7 h and the suitable solution pH should be above 3. Both Langmuir and Freundlich models fitted the data well and precipitation was found to be the dominant mechanism for the adsorption of both metals. Overall, grape vine shoots are confirmed to be a promising feedstock to produce biochar sorbents of heavy metals in wastewater with excellent performance, economic cost, and easy process. © 2018, Desalination Publications. All rights reserved.

语种： 英文

作者： Xiao, Guiqing;Zhou, Jiahao;Lu, Xiangyang;Huang, Rongfeng*;Zhang, Haiwen*

期刊： New Phytologist,2018年217(1):332-343 ISSN：0028-646X

通讯作者： Huang, Rongfeng;Zhang, Haiwen

作者机构： [Xiao, Guiqing; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Guiqing; Huang, RF; Zhang, Haiwen; Huang, Rongfeng; Zhou, Jiahao] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.

通讯机构： [Huang, RF; Zhang, HW] C;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China.

关键词： UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1);caspase-like activity;lesion;programmed cell death (PCD);reactive oxygen species (ROS);rice (Oryza sativa);uridine 5'-diphosphoglucoseglucose (UDPG)

摘要： Lesion mimic mutants are valuable to unravel the mechanisms governing the programmed cell death (PCD) process. Uridine 5′-diphosphoglucose-glucose (UDPG) functions as a signaling molecule activating multiple pathways in animals, but little is known about its function in plants. Two novel allelic mutants of spl29 with typical PCD characters and reduced pollen viability were obtained by ethane methyl sulfonate mutagenesis in rice cv Kitaake. The enzymatic analyses showed that UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) irreversibly catalyzed the decomposition of UDPG. Its activity was severely destroyed and caused excessive UDPG accumulation, with the lesion occurrence associated with the enhanced caspase-like activities in spl29-2. At the transcriptional level, several key genes involved in endoplasmic reticulum stress and the unfolded protein response were abnormally expressed. Moreover, exogenous UDPG could aggravate lesion initiation and development in spl29-2. Importantly, exogenous UDPG and its derivative UDP-N-acetylglucosamine could induce reactive oxygen species (ROS) accumulation and lesion mimics in Kitaake seedlings. These results suggest that the excessive accumulation of UDPG, caused by the mutation of UAP1, was a key biochemical event resulting in the lesion mimics in spl29-2. Thus, our findings revealed that UDPG might be an important component involved in ROS accumulation, PCD execution and lesion mimicking in rice, which also provided new clues for investigating the connection between sugar metabolism and PCD process. © 2017 The Authors. New Phytologist © 2017 New Phytologist Trust

语种： 英文

作者： 甘玉姿;肖贵;邓启云;吴俊;柏斌;...

期刊： 中国生物防治学报,2018年34(3):488-498 ISSN：2095-039X

作者机构： [甘玉姿] 湖南农业大学生物科学技术学院,长沙410128;[甘玉姿] 湖南杂交水稻研究中心,湖南410125;[甘玉姿] 国际水稻研究所,马尼拉0900;湖南杂交水稻研究中心,湖南,410125;湖南农业大学生物科学技术学院,长沙,410128

关键词： 稻瘟病菌;AVR-Pita 及其同源基因;序列分析;功能分析

摘要： 根据已克隆的AVR-Pita及其同源基因序列设计4对引物,对来自菲律宾的74个稻瘟病单孢菌株DNA进行PCR扩增和序列分析,研究了与水稻抗病基因Pi-ta互作的稻瘟病菌无毒基因AVR-Pita及其同源基因序列多态性与进化关系。结果显示,在42个菌株中扩增出AVR-Pita1基因目的条带,31个菌株中扩增出AVR-Pita3基因目的条带,55个菌株中扩增出AVR-Pita4基因目的条带,但所有供试菌株均未扩增出AVR-Pita2基因目的条带。对PCR产物进行测序分析,发现AVR-Pita1基因在菲律宾的生理小种中存在序列多态性,可划分为5个单倍型,共有10个多态性位点;AVR-Pita3和AVR-Pita4基因序列较保守,序列比对后没有发现多态性。对不同单基因系接种鉴定,发现除第3种AVR-Pita1单倍型外,含有AVR-Pita1单倍型1、2、4及5的生理小种均不能与Pi-ta基因互作,从而使Pi-ta单基因系IRBLta-CT2在接种后表现为感病。以上试验结果说明AVR-Pita1基因变异性较强,且序列改变后会导致基因功能的变化。

语种： 中文

作者： Li, Bin;Wang, Jin;Cheng, Xiaoyang;Liu, Yan;Yang, Yang;...

期刊： 科学通报(英文版),2018年63(24):1616-1625 ISSN：2095-9273

通讯作者： Tian, Yun;Yu, Ye

作者机构： [Tian, Yun; Zhu, Michael X.; Lu, Xiangyang; Yang, Yang; Li, Bin; Yang, Xiaona] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhu, Michael X.] Guangxi Normal Univ, Sch Chem & Pharmaceut Sci, State Key Lab Chem & Mol Engn Med Resources, Guilin 541004, Peoples R China.;[Cheng, Xiaoyang; Liu, Yan; Li, Bin; Wang, Jin; Yu, Ye] Shanghai Jiao Tong Univ, Inst Med Sci, Dept Pharmacol & Chem Biol, Sch Med, Shanghai 200025, Peoples R China.;[Guo, Changrun; Yu, Ye; Yang, Xiaona] China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing 210009, Jiangsu, Peoples R China.;[Niu, Youya] Hunan Univ Med, Dept Cell Biol & Genet, Huaihua 418000, Peoples R China.

通讯机构： [Tian, Yun] H;[Yu, Ye] S;[Yu, Ye] C;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Shanghai Jiao Tong Univ, Inst Med Sci, Dept Pharmacol & Chem Biol, Sch Med, Shanghai 200025, Peoples R China.

关键词： P2X receptors;Competitive inhibitors;NF110;Species-specificity;Subtype-selectivity

摘要： P2X receptors are a family of extracellular ATP-gated trimeric cation channels that is widely distributed in human tissues. Quite some drug candidates targeting P2X receptors have entered into preclinical or main phases of clinical trials, but many of them failed due to low subtype-selectivity or species differences in pharmacological activities between human and experimental animals. Here, we identified the distinct inhibitory efficacies of NF110, a competitive inhibitor, between the rat (rP2X3) and human (hP2X3) P2X3 receptors. We demonstrated that this difference is determined by two amino acids located in the dorsal fin (DF) domain of P2X3 receptors. As revealed by mutagenesis, metadynamics, and covalent modification, NF110-mediated rP2X3 inhibition may be through a filling in the cavity formed by the DF, left flipper (LF) and lower body (LB) to partially, rather than fully, occupy the ATP-binding pocket. Moreover, substitution of residues located in the DF and/or LF domains of the rP2X2 receptor, a NF110-insensitive subtype, with the equivalent amino acids of rP2X3, bestowed the sensitivity of rP2X2 to NF110. The critical roles of the DF and LF domains in channel gating of P2X and low conservativity in residue sequences of those two domains raise the possibility that small molecules differentially interacting with the residues of the DF and LF domains of different P2X receptors may modulate channel's activity in a subtype-selective manner. However, the possible species-specificity of P2X inhibitors/modulators makes it more complex when interpreting the preclinical data into clinical researches. Nevertheless, our data provide new insights into the subtype-selectivity of competitive inhibitors and their distinct potencies in the human and experimental animals, both of which are extremely important in the drug discovery of P2X receptors. © 2018 Science China Press

语种： 英文

作者： Chen, Xiaoyu;Tian, Yun*;Lu, Xiangyang*

期刊： Agronomy,2018年8(9):191- ISSN：2073-4395

通讯作者： Tian, Yun;Lu, Xiangyang

作者机构： [Tian, Yun; Tian, Y; Lu, XY; Lu, Xiangyang; Chen, Xiaoyu] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Tian, Y; Lu, XY] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

关键词： rice (Oryza sativa L.);seed;dormancy;thermosensitive genic male-sterile (TGMS) lines;pre-harvest sprouting (PHS);abscisic acid (ABA)

摘要： The pollen development of male-sterile lines should be conducted during the high temperature season to ensure the sterility of male-sterile line pollen and the safety of hybrid seed production in the breeding of thermosensitive genic male-sterile (TGMS) lines of early rice. However, the mature hybrid rice seeds are prone to pre-harvest sprouting (PHS) during hot, rainy days, which leads to the decrease of hybrid rice seed quality or a loss of utilization value. In this study, with an aim to bring a dormancy trait from the dormant genotype to the current low-dormant TGMS lines, a common wild rice “Chaling” (Oryza rufipogon), which has strong seed dormancy, a large black stigma, and a high exsertion rate, was hybridized with the early rice TGMS line “Zhu 1S,” which often exhibits PHS. After screening for early maturing and seed dormancy, the early rice “Yezao S” and “Yezhuzao S,” with the characteristic of seed dormancy, were selected. The critical temperature of high temperature sterility for these two male-sterile lines was below 23.5 °C. The stigma of “Yezao S” was black and its exsertion rate was 79.9%, while “Yezhuzao S” had a colorless stigma with an exsertion rate of 76.8%. The expression of the genes OsNCEDs in “Yezao S” and “Yezhuzao S” were significantly higher than that in “Zhu 1S” the 15th day after heading, and the abscisic acid (ABA) content was also higher than that of “Zhu 1S” the 20th day after heading. The results indicated that introducing the dormancy characteristics from wild rice “Chaling” into the early rice TGMS lines was an effective way to prevent PHS.

语种： 英文

作者： 朱兆静;潘虎;郭俊;卢向阳;王翀;...

期刊： 江苏农业科学,2018年46(19):12-16 ISSN：1002-1302

作者机构： [潘虎; 王翀; 郭俊; 田云; 卢向阳; 朱兆静] 湖南省农业生物工程研究所;湖南农业大学生物科学技术学院;西藏自治区农牧科学院农业质量标准与检测研究所

关键词： 纤维小体;多酶复合体;组装模式;生物质;人工纤维小体

摘要： 纤维素作为地球上数量最大的可再生资源,因其难以降解,造成了以纤维素为主的生物资源的极大浪费.纤维小体(cellulosome)是厌氧生物产生的胞外多酶复合体,能够高效地降解纤维素,是开发利用纤维素的重要途径之一.目前,对纤维小体的研究主要集中在基因和基因组水平,而对纤维小体多酶复合体的结构和功能方面的研究相对缺乏.本文结合国内外对纤维小体最新的研究状况,综述了纤维小体多酶复合体的组装模式及其功能,并讨论了纤维小体用于生物质降解中存在的主要问题,以期为人工纤维小体的改造和纤维素资源的利用提供更多的理论基础.

语种： 中文

作者： 周智;张永丽;王变变;盖淑杰;王若仲;...

期刊： 植物学报,2018年53(4):502-508 ISSN：1674-3466

作者机构： 湖南农业大学农学院, 长沙, 410128;湖南农业大学理学院, 长沙, 410128;湖南农业大学生物科学技术学院, 长沙, 410128;[周智] 湖南农业大学农学院, 长沙, 410128 湖南农业大学理学院, 长沙, 410128 湖南农业大学生物科学技术学院, 长沙, 410128;[张永丽; 王变变] 湖南农业大学理学院, 长沙, 410128

关键词： 荧光粉激发型LED;生长;模式植物;光谱组成

摘要： 在室内人工光源照射条件下,探究150μmol·m~(–2)·s~(–1)光强下不同比例的红蓝光对拟南芥(Arabidopsis thaliana)生长发育的影响。以哥伦比亚(Columbia-0)野生型拟南芥为研究对象,采用荧光粉激发型LED作为植物生长光源,以SrSiAlN3为红色基底,调节荧光粉添加量获得不同红蓝光谱,考察不同光照条件下拟南芥萌发率、根长、株高、叶绿素含量和相对电导率等参数的变化规律。结果表明,在荧光粉激发型LED光照调节下的拟南芥具有更高的萌发率、根长、株高、叶绿素含量和相对电导率,且在红蓝光质比为2:1时萌发率(95.63%)和叶绿素含量(26.7)最高;在红蓝光质比为4:5时根长(4.19 cm)较长;在红蓝光质比为4:1时株高(15.5 cm)较高;在红蓝光质比为4:5时相对电导率(40.5 S·m~(–1))较大。研究结果表明相对平衡的光质(红蓝光质比为4:1)有利于拟南芥生长发育,且减少蓝光比例对根系生长及叶绿素积累有一定的促进作用。研究表明不同光谱对模式植物拟南芥的生长发育有较明显的影响,改变光谱组成可以对植物的生长发育起不同程度的调控作用。

语种： 中文

作者： 戴林建;盖淑杰;周子迁;邓子恒;刘晓颖;...

期刊： 核农学报,2018年32(7):1442-1447 ISSN：1000-8551

作者机构： [戴林建; 盖淑杰; 邓子恒] 湖南农业大学农学院;[周子迁; 卢向阳; 周智; 卢向阳] 湖南农业大学理学院;[周智; 卢向阳] 湖南农业大学生物科学技术学院

关键词： 荧光粉;LED植物光源;烟草育苗;补光

摘要： 为解决湖南烟区冬季大棚育苗常遇到的低温寡照问题,以云烟87为试验材料,利用自制不同波段的荧光粉组装成不同光谱的LED植物光源器件,开展不同光质LED对苗期的补光试验,研究LED不同光质对烟草育苗及烟苗生长的主要生理指标的影响,同时采用荧光灯进行对照试验。结果表明,采用LED补光,烟苗出苗率、苗高、茎围、最大叶长、最大叶宽、茎高、叶绿素、根系活力较荧光灯(对照组)显著增加。最佳LED补光可以缩短育苗时间至47 d,较对照组提前36 d,显著增强了成苗素质。在荧光粉激发型LED植物光源器件中,以发射峰位于630 nm波段荧光粉的器件补光效果最好,其所育烟苗素质较对照组高,能达到移栽壮苗要求,可以满足烟苗冬季补光育苗的条件。本研究为烤烟育苗人工补光提供了理论依据。

语种： 中文