Biotransformation and tissue distribution of protopine and allocryptopine and effects of Plume Poppy Total Alkaloid on liver drug-metabolizing enzymes
作者：
Huang, Ya-Jun;Cheng, Pi;Zhang, Zhuo-Yi;Tian, Shi-Jie;Sun, Zhi-Liang;...
期刊：
Scientific Reports ,2018年8(1):537 ISSN：2045-2322
通讯作者：
Liu, Zhao-Ying
作者机构：
[Tian, Shi-Jie; Sun, Zhi-Liang; Zeng, Jian-Guo; Liu, Zhao-Ying; Cheng, Pi; Huang, Ya-Jun] Hunan Agr Univ, Vet Herbal Med Resources & Initiat, Natl & Local Union Engn Res Ctr, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying; Zhang, Zhuo-Yi; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr ofVeterinary Drug, Changsha 410128, Hunan, Peoples R China.;[Zeng, Jian-Guo; Liu, Zhao-Ying] Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Vet Herbal Med Resources & Initiat, Natl & Local Union Engn Res Ctr, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr ofVeterinary Drug, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.
摘要：
In this study, the biotransformation in the plasma, urine and feces of rats following oral administration of protopine (PRO) and allocryptopine (ALL)were explored using HPLC-QqTOF MS. An HPLC-MS/MS method for the determination of tissues was developed and applied to the tissue distribution study in rats following intragastric administration of Plume Poppy Total Alkaloid for 3 weeks. A total of ten PRO metabolites and ten ALL metabolites were characterized in rats in vivo. Among these metabolites, six PRO metabolites and five ALL metabolites were reported for the first time. The predicated metabolic pathways including ring cleavage, demethylation following ring cleavage, and glucuronidation were proposed. The low-concentration residue of PRO and ALL in various tissues was detected at 24 h and 48 h after dosing, which indicated that both compounds could be widely distributed in tissues and exist as low levels of residue. The activities of erythromycin N-demethylase, aminopyrine N-demethylase and NAD (P)H quinone oxidoreductase in female rats can be induced post-dose, but these activities were inhibited in male rats. The proposed biotransformation and residues of PRO and ALL and their effects on enzymes may provide a basis for clarifying the metabolism and interpreting pharmacokinetics.
语种：
英文
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Characterization of N-methylcanadine and N-methylstylopine metabolites in rat liver S9 by high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry
作者：
Zuo, Meng-Ting;Liu, Sha-Sha;Lin, Li;Wang, Zi-Yuan;Bai, Xia;...
期刊：
Rapid Communications in Mass Spectrometry ,2018年32(23):2047-2054 ISSN：0951-4198
通讯作者：
Liu, Zhao-Ying
作者机构：
[Bai, Xia; Liu, Sha-Sha; Wang, Zi-Yuan; Sun, Zhi-Liang; Liu, Zhao-Ying; Zuo, Meng-Ting] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Bai, Xia; Liu, Sha-Sha; Sun, Zhi-Liang; Liu, Zhao-Ying; Lin, Li; Zuo, Meng-Ting] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要：
Rationale: N-Methylcanadine and N-methylstylopine are two types of isoquinoline alkaloids which are considered to be the main medicinally active constituents of the genus Papaveraceae. However, to date, no metabolism studies of N-methylcanadine and N-methylstylopine have been reported. Therefore, the purpose of the present study was to investigate the in vitro metabolism of these two alkaloids in rat liver S9. Methods: N-Methylcanadine or N-methylstylopine was incubated with rat liver S9 for 1 h, and then the incubation mixture was processed with 15% trichloroacetic acid. High-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (HPLC/QqTOF-MS) as a reliable analytical method was used. The structural characterization of these metabolites was performed by the combination of the accurate MS/MS spectra and the known elemental composition. Results: As a result, a total of four metabolites of N-methylcanadine and five metabolites of N-methylstylopine in rat liver S9 were tentatively identified. The cleavage of the methylenedioxy group of the drugs was the main metabolic pathway of N-methylcanadine and N-methylstylopine. Conclusions: The present study is the first in vitro metabolic investigation of N-methylcanadine and N-methylstylopine in rat liver S9 using a reliable HPLC/QqTOF-MS method. The metabolic pathways of N-methylcanadine and N-methylstylopine are tentatively proposed. This work lays the foundation for the in vivo metabolism of the two compounds in animals. © 2018 John Wiley & Sons, Ltd.
语种：
英文
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Paeoniflorin regulates GALNT2-ANGPTL3-LPL pathway to attenuate dyslipidemia in mice
作者：
Xiao, Hong-Bo* ;Liang, Lin;Luo, Zhi-Feng;Sun, Zhi-Liang
期刊：
European Journal of Pharmacology ,2018年836:122-128 ISSN：0014-2999
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Liang, Lin] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Luo, Zhi-Feng] Xiangnan Univ, Dept Basic Med, Chenzhou 423000, Peoples R China.;[Sun, Zhi-Liang] Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
摘要：
N-acetylgalactosaminyltransferase 2-Angiopoietin-like protein 3-lipoprotein lipase (GALNT2-ANGPTL3-LPL) pathway may be a useful pharmacologic objective for dyslipidemia. The present study was conducted to test the effect of paeoniflorin, a monoterpene Glycoside, on dyslipidemia in mice. Fifty mice were randomly divided into five groups (n = 10): three groups of apolipoprotein E-null (ApoE-/-) mice treated with paeoniflorin (10 or 20 or 30 mg/kg/day), untreated ApoE-/- mice group, and C57BL/6J control group. Six weeks after treatment, expression of hepatic ANGPTL3, hepatic GALNT2 and adipose tissue LPL, lipid levels in the liver and blood were quantified. Treatment with paeoniflorin (10 or 20 or 30 mg/kg) obviously down-regulated expression of ANGPTL3 and up-regulated expressions of GALNT2 and LPL concomitantly with elevated plasma high-density lipoprotein cholesterol level, reduced plasma concentrations of low-density lipoprotein cholesterol, total cholesterol, triglyceride, malonaldehyde, and 8-isoprostane. The present results suggest that paeoniflorin regulates GALNT2-ANGPTL3-LPL pathway to attenuate dyslipidemia in mice. © 2018 Elsevier B.V.
语种：
英文
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Kaempferol modulates Angiopoietin-like protein 2 expression to lessen the mastitis in mice
作者：
Xiao, Hong-Bo* ;Sui, Guo-Guang;Lu, Xiang-Yang;Sun, Zhi-Liang
期刊：
Pharmacological Reports ,2018年70(3):439-445 ISSN：1734-1140
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Sui, Guo-Guang] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Univ, Hunan Prov Univ Key Lab Agr Biochem & Biotransfor, Changsha, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Coinnovat Ctr Ultilizat Bot Funct Ingredien, Changsha, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
关键词：
Kaempferol;Angiopoietin-like protein 2;Murine mastitis
摘要：
Background: Mastitis is inflammation of a breast (or udder). Angiopoietin-like protein 2 (ANGPTL2) has been found as a key inflammatory mediator in mastitis. Purpose of this research was to investigate the mechanisms about repressing effect of kaempferol on mastitis. Methods: Forty mice were randomly divided into 4 groups (n = 10): C57BL/6J control mice, untreated murine mastitis, 10 mg/kg kaempferol treated murine mastitis (ip), and 30 mg/kg kaempferol treated murine mastitis (ip). Primary cultured mouse mammary epithelial cells (MMEC) were indiscriminately divided into seven groups including control group, 10 mu mol/L vehicle of kaempferol group, 10 mu mol/L kaempferol treated group, 20 mu g/mL LPS treated group, 1 mmol/L kaempferol plus LPS treated group, 3 mu mol/L kaempferol plus LPS treated group, and 10 mu mol/L kaempferol plus LPS treated group. Results: In murine mastitis, kaempferol (10 or 30 mg/kg) treatment prevented mastitis development, decreased myeloperoxidase (MPO) production, interleukin (IL)-6 level, tumour necrosis factor-alpha (TNF-alpha) concentration, and ANGPTL2 expression. In MMEC, kaempferol (1, 3 or 10 mu M) reduced MPO production, TNF-alpha concentration, IL-6 level, and ANGPTL2 expression. Conclusions: The results in present study show that kaempferol modulates the expression of ANGPTL2 to lessen the mastitis in mice. (c) 2018 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.
语种：
英文
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Development and in-house validation of a sensitive LC–MS/MS method for simultaneous quantification of gelsemine, koumine and humantenmine in porcine plasma
作者：
Yang, Kun;Long, Xue-Ming;Liu, Yan-Chun;Chen, Fu-Hua;Liu, Xiao-Feng;...
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2018年1076:54-60 ISSN：1570-0232
通讯作者：
Sun, Zhi-Liang;Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Liu, Yan-Chun; Liu, Xiao-Feng] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Chen, Fu-Hua; Long, Xue-Ming] Inst Hunan Prov Vet Drugs & Feed Supervis, Changsha 410006, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Sun, ZL; Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Sun, ZL; Liu, ZY] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
Gelsemine;Gelsemium elegans;Humantenmine;Indole alkaloid;Koumine;Plasma
摘要：
Three monomers of G. elegans indole alkaloids (gelsemine, koumine and humantenmine) were simultaneously detected in porcine plasma for the first time with the development and validation of a sensitive and reliable LC-ESI-MS/MS method. Using a gradient mobile phase at a constant flow rate of 0.2 mL/min via electrospray ionization (positive ion mode) in a multiple reaction monitoring (MRM) scan, gelsemine, koumine and humantenmine were eluted, separated and detected at an appropriate retention time. The porcine plasma was prepared using protein precipitation with 1% formic acid-acetonitrile: methanol (2:1, v/v). Using matrix-matched calibration curves and weighted least squares linear regression, a good linearity (r<sup>2</sup>>0.99) was achieved with a concentration range of 0.1–200 μg/L for gelsemine, koumine and humantenmine;estimated LOD and LOQ values were 0.10 μg/L and 0.2 μg/L, respectively. The mean of the recoveries was in the range of 82.68–100.35% of porcine plasma at four different levels, and the intra-day and inter-day precision (CV) were lower than 15% with a range of 2.46–8.76% and 2.73–10.83%, respectively. The proposed method has proved to be suitable for accurate, quantitative determination of gelsemine, koumine and humantenmine in porcine plasma.<br/> ©2018
语种：
英文
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钩吻药效学的研究进展
作者：
张志阳;伍勇;孙志良
期刊：
中国兽医杂志 ,2018年54(4):57-60 ISSN：0529-6005
作者机构：
湖南农业大学动物医学院,湖南长沙,410128;湖南农业大学动物医学院,湖南长沙410128;湖南省兽药工程技术研究中心,湖南长沙410128;[张志阳; 孙志良; 伍勇] 湖南农业大学
关键词：
钩吻碱;实体瘤;钩吻素子;钩吻生物碱
摘要：
钩吻,又名断肠草、大茶药等,是马钱科藤本植物[1].《神农本草》谓其“破积,脚膝痹痛,四肢拘挛、恶疮疥虫,鸟兽”;《本草纲目》记载“钩吻主金疮、乳痈、中恶风、咳逆上气,鬼痊蛊毒”.有报道称,钩吻对细胞周期有一定的影响,它能够阻止LoVo细胞由G1期向S期的转化,从而诱导细胞凋亡[2].最近的研究显示,钩吻及其生物碱的镇痛作用,主要通过脊髓的α3-甘氨酸受体/四氢孕酮途径来实现的[3].钩吻能够通过减少丙二醛(MDA)和一氧化氮(NO)的产生,提高过氧化氢酶(CAT),超氧化物歧化酶(SOD)和谷胱甘肽(GSH)等酶活性,从而降低高脂引起的氧化应激反应[5],钩吻素子能够通过调节NF-κB和MAPK信号通路抑制LPS诱导的RAW264.7细胞炎症因子的过度释放,说明其具有较好的体外抗炎作用[6-7].过去民间因钩吻毒性大,主要以外用为主,能祛风,消肿拔毒,杀虫止痒;目前研究发现有抑制癌症、保护心血管系统、造血、保护炎症损伤、扩张瞳孔、缓解疼痛及畜禽复壮等药理作用,现在对钩吻化学成分的研究虽有报道,但不够广泛,因其开发具有广阔的市场前景,为了促进对钩吻的应用,现就其化学成分、药理作用和毒理作用综述如下.
语种：
中文
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动物专用抗生素泰地罗新
作者：
杨昆;孙志良;隆雪明
期刊：
动物医学进展 ,2018年39(3):110-114 ISSN：1007-5038
作者机构：
湖南农业大学动物医学院;[隆雪明] 湖南省兽药饲料监察所;湖南省兽药工程技术研究中心;[杨昆; 孙志良] 湖南农业大学
关键词：
泰地罗新;合成;药理学;耐药性
摘要：
泰地罗新是新型动物专用的半合成大环内酯类抗生素,用于治疗由副猪嗜血杆菌、胸膜肺炎放线杆菌等细菌引起的呼吸道疾病,比泰乐菌素在抑制猪、牛呼吸道致病菌展现出更好的效果。该药吸收迅速,半衰期长,生物利用度高,性质稳定,具有较好的开发价值。然而,从2012年研发问世至今,该药在我国依旧未被批准应用于兽医临床。通过对泰地罗新合成、抗菌作用及机制、药物代谢动力学、检测方法和残留以及耐药性等方面的特点及研究概况进行总结,以期为泰地罗新药理和制剂学研究提供参考,同时为泰地罗新在兽医临床上应用提供基本依据。
语种：
中文
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钩吻素子对LPS诱导RAW264.7细胞炎症的作用
作者：
袁志航;袁慧;邬静;陈小军;刘兆颖;...
期刊：
中国兽医学报 ,2017年37(8):1553-1557 ISSN：1005-4545
作者机构：
湖南农业大学动物医学院, 湖南兽药工程技术研究中心, 湖南, 长沙, 410128;湖南农业大学动物医学院, 湖南, 长沙, 410128;[袁志航; 邬静; 陈小军; 刘兆颖; 孙志良] 湖南农业大学动物医学院, 湖南兽药工程技术研究中心, 湖南, 长沙, 410128;[袁慧] 湖南农业大学动物医学院, 湖南, 长沙, 410128
关键词：
钩吻素子;脂多糖;炎症;巨噬细胞
摘要：
为揭示钩吻素子的体外抗炎作用机理,利用硝酸还原酶法和ELISA法研究了不同质量浓度(100,200,400mg/L)的钩吻素子对一氧化氮(NO)和细胞因子白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)分泌的影响,运用了QPCR的方法检测了钩吻素子对诱导型一氧化氮合成酶(iNOS)、IL-1β、IL-6和TNF-αmRNA表达水平的影响,并运用Western blot法检测了钩吻素子对iNOS蛋白表达的影响。结果显示,100,200,400mg/L质量浓度的钩吻素子能够极显著的抑制RAW264.7细胞NO的产生以及IL-1β、IL-6和TNF-α的分泌(P<0.01),同时能够剂量依赖性的降低iNOS、IL-1β、IL-6和TNF-α的mRNA水平,抑制脂多糖(LPS)引起的iNOS蛋白表达的升高(P < 0.01)。由此推论,钩吻素子可能通过抑制炎症因子的分泌,减少NO的释放,下调iNOS、IL-1β、IL-6和TNF-α的mRNA水平,抑制iNOS蛋白过度表达达到其抗炎作用。
语种：
中文
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血根碱亚慢性毒性试验组织病理学观察
作者：
伍勇;肖倩文;邢东梅;宋璐琳;张孜婕;...
期刊：
中兽医医药杂志 ,2017年36(4):44-46 ISSN：1000-6354
作者机构：
[张孜婕; 伍勇] 湖南农业大学东方科技学院;湖南农业大学动物医学院;湖南省兽药工程技术研究中心;[宋璐琳; 肖倩文; 孙卓; 邢东梅; 孙志良] 湖南农业大学
关键词：
血根碱;小鼠;脏器指数;组织病理学
摘要：
观察血根碱亚慢性毒性试验对小鼠脏器的组织病理学变化,为将血根碱应用于兽医临床提供实验依据.将血根碱分别按高剂量[21 mg/(kg· d)]、低剂量[2.1 mg/(kg· d)]以及蒸馏水给小鼠连续灌胃30 d后,计算脏器指数,并观察各脏器的组织病理学变化.结果显示,与空白组相比,21 mg/(kg·d)剂量组小鼠脾脏与肾脏出现组织病理学变化,2.1 mg/(kg·d)剂量组各脏器未见病理变化,且小鼠脾脏指数降低,其他脏器指数无变化.本研究对血根碱的亚慢性毒性进行了初步评价,为血根碱的临床应用提供理论基础.
语种：
中文
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Structural elucidation of koumine metabolites by accurate mass measurements using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry
作者：
Xiao, Sa;Huang, Ya-Jun;Sun, Zhi-Liang;Liu, Zhao-Ying*
期刊：
Rapid Communications in Mass Spectrometry ,2017年31(3):309-314 ISSN：0951-4198
通讯作者：
Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Liu, Zhao-Ying; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Xiao, Sa; Huang, Ya-Jun] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
摘要：
Rationale: Koumine is one of the major components of total alkaloids from Gelsemium. Koumine possesses a variety of interesting pharmacological effects, including anti-tumor, anti-inflammatory, and anxiolytic activities. It might be a promising lead drug because of its pharmacological activities and mild toxicity. However, little information is available on the metabolism of koumine. Methods: A rapid and accurate high-performance liquid chromatography/quadrupole-time-of-flight (HPLC/QqTOF) mass spectrometry method was applied to characterize koumine metabolites. Multiple scans of koumine metabolites, which were formed in rat liver S9, were automatically performed simultaneously through auto MS/MS mode acquisition in only a 30-min analysis. The structural elucidation of these metabolites was performed by comparing their changes in accurate molecular masses and product ions with those of the parent drug or metabolites. Results: As a result, a total of eleven metabolites of koumine were identified, of which nine new metabolites were found. The present results showed that the N-demethylenation, hydrogenation and the oxidation were the three main metabolic pathways of koumine. Conclusions: This was the first investigation of in vitro metabolism of koumine in rat liver S9 using a sensitive and specific HPLC/QqTOF-MS method. The possible metabolic pathways of koumine were tentatively proposed based on the structural elucidations of these metabolites. This work may be useful in the in vivo metabolism of koumine in animals and humans. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.
语种：
英文
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钩吻化学成分初步研究
作者：
张志阳;阳洁;王其龙;左香溢;伍勇;...
期刊：
中兽医医药杂志 ,2017年36(6):20-23 ISSN：1000-6354
作者机构：
湖南农业大学动物医学院,湖南长沙,410128;湖南农业大学动物医学院,湖南长沙410128;湖南省兽药工程技术研究中心,湖南长沙410128;[左香溢; 张志阳; 王其龙; 袁志航; 阳洁; 孙志良; 伍勇] 湖南农业大学
关键词：
钩吻;化学成分;试管法;圆形滤纸层析法
摘要：
目的：对钩吻的化学成分进行初步研究。方法：采用试管法和圆形滤纸层析法对钩吻的提取液可能含有的化学成分进行初步确定。结果：钩吻可能含有氨基酸、皂苷、有机酸、生物碱、黄酮及其苷类、鞣质、强心苷、植物甾醇、三萜、油脂、糖等化学成分。结论：初步确定了钩吻可能含有的化学成分,为钩吻的深度利用和开发提供依据。
语种：
中文
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火参果化学成分初步研究
作者：
张志阳;唐模欢;彭璟;夏雨;伍勇;...
期刊：
中兽医医药杂志 ,2017年36(5):11-14 ISSN：1000-6354
作者机构：
湖南农业大学动物医学院,湖南长沙,410128;湖南农业大学动物医学院,湖南长沙410128;湖南省兽药工程技术研究中心,湖南长沙410128;[张志阳; 唐模欢; 袁志航; 彭璟; 夏雨; 孙志良; 伍勇] 湖南农业大学
关键词：
火参果;化学成分;试管法;圆形滤纸层析法
摘要：
目的:对火参果的化学成分进行初步研究。方法:采用试管法和圆形滤纸层析法对火参果提取液进行研究,对其可能含有的化学成分进行初步确定。结果:火参果中含有氨基酸、有机酸、生物碱、黄酮及苷类、鞣质、植物甾醇、三萜、油脂、糖、氰苷成分。结论:初步确定了火参果可能含有的化学成分,为火参果的深度利用和开发提供依据。
语种：
中文
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钩吻的历史溯源及植物学特征
作者：
张卓亿;代佳娣;杨昆;孙志良;刘兆颖
期刊：
中兽医医药杂志 ,2017年36(6):78-82 ISSN：1000-6354
作者机构：
湖南省植物功能协同创新中心湖南农业大学动物医学院,湖南长沙,410128;[代佳娣; 刘兆颖; 张卓亿; 杨昆; 孙志良] 湖南农业大学
关键词：
钩吻;植物学特征;传统应用
摘要：
钩吻具有广泛的药理活性,在中兽药领域的应用前景广阔。自古以来,国内外对钩吻的记载纷繁多样,说法不一。本文就钩吻的历史溯源、植物学特征及传统应用方面进行梳理,为钩吻的安全使用及开发提供理论依据。
语种：
中文
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Comprehensive identification and structural characterization of target components from Gelsemium elegans by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry based on accurate mass databases combined with MS/MS spectra
作者：
Liu, Yan-Chun;Xiao, Sa;Yang, Kun;Ling, Li;Sun, Zhi-Liang;...
期刊：
JOURNAL OF MASS SPECTROMETRY ,2017年52(6):378-396 ISSN：1076-5174
通讯作者：
Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Yang, Kun; Liu, Zhao-Ying; Liu, Yan-Chun; Ling, Li; Xiao, Sa] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Coll Vet Med, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
accurate mass databases;Gelsemium;quadrupole time-of-flight mass spectrometry;traditional Chinese medicine;alkaloid
摘要：
This study reports an applicable analytical strategy of comprehensive identification and structure characterization of target components from Gelsemium elegans by using high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QqTOF MS) based on the use of accurate mass databases combined with MS/MS spectra. The databases created included accurate masses and elemental compositions of 204 components from Gelsemium and their structural data. The accurate MS and MS/MS spectra were acquired through data-dependent auto MS/MS mode followed by an extraction of the potential compounds from the LC-QqTOF MS raw data of the sample. The same was matched using the databases to search for targeted components in the sample. The structures for detected components were tentatively characterized by manually interpreting the accurate MS/MS spectra for the first time. A total of 57 components have been successfully detected and structurally characterized from the crude extracts of G. elegans, but has failed to differentiate some isomers. This analytical strategy is generic and efficient, avoids isolation and purification procedures, enables a comprehensive structure characterization of target components of Gelsemium and would be widely applicable for complicated mixtures that are derived from Gelsemium preparations. Copyright ©2017 John Wiley &Sons, Ltd. Copyright ©2017 John Wiley & Sons, Ltd.
语种：
英文
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四种猪可食性组织中巴氯芬定量/确证方法研究
作者：
杨昆;周宇俊;易远胜;刘艳纯;孙志良;...
作者机构：
[杨昆; 周宇俊; 易远胜; 刘艳纯; 孙志良; 刘兆颖] 湖南农业大学动物医学院
会议名称：
中国毒理学会兽医毒理学委员会与中国畜牧兽医学会兽医食品卫生学分会联合学术研讨会暨中国毒理学会兽医毒理学委员会第5次全国会员代表大会
会议时间：
2017-09-22
会议地点：
中国北京
会议论文集名称：
中国毒理学会兽医毒理学委员会与中国畜牧兽医学会兽医食品卫生学分会联合学术研讨会暨中国毒理学会兽医毒理学委员会第5次全国会员代表大会会议论文集
关键词：
巴氯芬;定量;可食性组织;液质联用
摘要：
【目的】本试验采用三重四级杆串联质谱,对具有镇静、催眠、促生长作用而未获得批准应用于兽药临床的巴氯芬进行定量/确证方法研究,以期能应用于猪可食性组织中对巴氯芬残留监控。【方法】采用酸性乙酸铵将巴氯芬水解游离后,沉淀可溶性蛋白后,用异丙醇-乙酸乙酯将游离型巴氯芬液液萃取,进一步用阳离子SPE固相萃取净化巴氯芬,以m/z 214.1>179.0*和m/z214·1>151·0为反应监测离子对,采用多反
语种：
中文
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EGCG抑制饮食性高脂血症大鼠Angptl3-LPL信号通路的研究
作者：
蔡杰;王宇;伍勇;孙志良;李元义;...
期刊：
中国兽医学报 ,2017年37(3):523-529 ISSN：1005-4545
作者机构：
湖南农业大学动物医学院, 湖南, 长沙, 410128;湖南农业大学动物医学院, 国家植物功能成分利用工程技术研究中心, 湖南, 长沙, 410128;湖南农业大学动物医学院, 国家植物功能成分利用工程技术研究中心;[孙志良; 陈小军] 湖南省兽药工程中心, 湖南, 长沙, 410128;[蔡杰; 王宇; 李元义] 湖南农业大学动物医学院, 湖南, 长沙, 410128
关键词：
高脂血症;Angptl3-LPL信号通路
摘要：
本研究旨在探讨表没食子儿茶素没食子酸酯(EGCG)的降脂作用与Angptl3-LPL信号通路之间的关系。通过高脂饲料喂养建立高脂血症大鼠动物模型,将正常大鼠按体质量和体脂水平随机分为正常对照组、高脂模型组、 EGCG高剂量组(EGCG-H,30 mg·kg~(-1)·d~(-1))、EGCG低剂量组(EGCG-L、15 mg·kg~(-1)·d~(-1))、T0901317组、 EGCG-T0901317组(EGCG,30 mg·kg~(-1)·d~(-1))。连续灌胃8周后,用生化法检测血清中TG、TC、LDL-C含量, qPCR法检测脂肪组织中脂蛋白脂肪酶(LPL)mRNA和肝组织中Angptl3 mRNA相对表达量,Western blot检测蛋白表达水平。给大鼠口服选择性的LXRs激动剂T0901317后测定肝脏Angptl3 mRNA和蛋白表达水平,评价EGCG对T0901317抑制剂的作用。结果表明,与高脂模型组相比,EGCG(30、15 mg·kg~(-1)·d~(-1))能有效降低大鼠的血脂水平,明显抑制肝脏Angptl3 mRNA和蛋白表达、上调脂肪组织LPL mRNA和蛋白表达水平(P<0.05或P<0.01),且EGCG(30 mg·kg~(-1)·d~(-1))可有效抑制由T0901317引起的Angptl3 mRNA和蛋白的表达。本研究从一个新的角度揭示了EGCG通过抑制Angptl3-LPL信号通路来防治与高脂相关性疾病的可能机制,所以以Angptl3- LPL信号通路为调节靶标,可能成为治疗血脂紊乱的一条新途径。
语种：
中文
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Determination of Baclofen Residue in Muscle, Liver, Kidney and Fat of Swine by Liquid Chromatography-Tandem Mass Spectrometry
作者：
Yang, Kun;Zhou, Yu-Jun;Chen, Fu-Hua;Long, Xue-Ming;Kuang, Guang-Wei;...
期刊：
Food Analytical Methods ,2017年10(12):3866-3873 ISSN：1936-9751
通讯作者：
Sun, Zhi-Liang;Liu, Zhao-Ying
作者机构：
[Sun, Zhi-Liang; Zhou, Yu-Jun; Yang, Kun; Liu, Zhao-Ying; Sun, ZL] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.;[Kuang, Guang-Wei; Chen, Fu-Hua; Long, Xue-Ming] Inst Hunan Prov Vet Drugs & Feed Supervis, Changsha 410006, Hunan, Peoples R China.
通讯机构：
[Sun, ZL; Liu, ZY] H;Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha 410128, Hunan, Peoples R China.
关键词：
Baclofen;Residue;LC-MS/MS;Validation method;Edible animal tissues
摘要：
Baclofen was illegally used in veterinary clinical medicine as a growth-promoting agent. To date, few methods have been developed for the monitoring of baclofen in animal tissues. In this study, a sensitive and efficient liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to identify and quantify baclofen in the muscle, liver, kidney, and fat of swine was developed and validated. Baclofen was extracted from tissues with ammonium acetate buffer (pH 5.2) and isolated with isopropyl-ethyl acetate (4:6, v/v). Then, a solid phase extraction using MCX cartridge was used to clean up the extracts. The elution was evaporated to dryness and reconstituted with water/methanol (90:10 v/v). All samples were determined by LC-MS/MS system through positive ionization in a multiple reaction monitoring (MRM) mode. The proposed method was validated by evaluation of specificity, linearity, recovery, accuracy, precision, LOD, and LOQ values according to Commission Decision 2002/657/EC. Estimated limit of quantification for baclofen in the muscle, liver, kidney, and fat of this method was 1.00 μg/kg, respectively. The mean intra- and inter-day assay accuracies fell within a range 88.5–93.9% and 86.2–93.2%, respectively. The mean intra- and inter-day precisions were 1.78 and 4.95% (RSD < 15%), respectively. The proposed method has proved to be suitable for accurate quantitative determination of baclofen for residue analysis.<br/> ©2017, Springer Science+Business Media, LLC.
语种：
英文
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Fingerprint analysis of Gelsemium elegans by HPLC followed by the targeted identification of chemical constituents using HPLC coupled with quadrupole-time-of-flight mass spectrometry
作者：
Liu, Yan-Chun;Lin, Li;Cheng, Pi;Sun, Zhi-Liang;Wu, Yong;...
期刊：
Fitoterapia ,2017年121:94-105 ISSN：1971-551X
通讯作者：
Liu, Zhao-Ying
作者机构：
[Wu, Yong; Sun, Zhi-Liang; Liu, Zhao-Ying; Liu, Yan-Chun; Lin, Li] Hunan Agr Univ, Hunan Engn Res Ctr Vet Drug, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying; Lin, Li; Cheng, Pi] Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Hunan, Peoples R China.;[Wu, Yong; Sun, Zhi-Liang; Liu, Zhao-Ying] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhao-Ying] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Zhao-Ying] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.
关键词：
14-Acetoxy-15-hydroxygelsenicine (PubChem CID:102185548);14-Acetoxygelsenicine (PubChem CID:102185547);14-Hydroxygelsenicine (PubChem CID:14217347);14-hydroxygelsemicine (PubChem CID:22217388);19R-Hydroxydihydrogelsemine (PubChem CID:101601182);7-Deoxygelsemide or 9-Deoxygelsemide (PubChem CID:101413879 or 46176622);Alkaloids, fingerprint;Chemical constituents;Dehydrokoumidine (PubChem CID:119077162);Ferulic acid (PubChem CID:445858);GS-2 (PubChem CID:12070888);GSIR-1 (PubChem CID:11105986);Gallic acid (PubChem CID:370);Gelsemicine (PubChem CID: 54624 28);Gelsemine (PubChem CID:5390854);Gelsemiol (PubChem CID:10375526);Gelsemiol-3-glucoside (PubChem CID:101098709);Gelsemium elegans;Gelsenicine (PubChem CID: 21123652);Gelseoxazolidinine (PubChem CID:102297300);Gelsevirine (PubChem CID:14217344);HPLC;Humantenine (PubChem CID:44593672);11-hydroxyhumantenine (PubChem CID:5318224);Koumidine (PubChem CID:44584550);Koumine (PubChem CID:91895267);Mass spectrometry;Pyrogallol (PubChem CID:105);Semperoside (PubChem CID:11439812);Sempervirine (PubChem CID:168919)
摘要：
Gelsemium elegans, which is a genus of the family Loganiaease, is commonly used as a traditional medicine for promoting animal growth and treating rheumatoid arthritis pain and neuropathic pain, among others. In this study, we first established a valid high-performance liquid chromatography (HPLC) method for the fingerprint analysis of Gelsemium elegans samples. Then, the comprehensive detection of chemical constituents from the samples was performed using HPLC coupled with quadrupole-time-of-flight mass spectrometry. The similarity evaluation results showed that location and area differences influenced the quality of the samples. An efficient strategy for the rapid targeted identification of chemical components was matching with a developed Gelsemium database. As a result, the accurate elemental compositions and known structures of compounds are found as hits. This process facilitated the structural identification of compounds combined with the accurate mass measurement of product ions and fragmentation behaviors. Consequently, 41 components including six alkaloids and non-alkaloids were systematically identified from Gelsemium elegans. The results showed that at least seven relatively major components existing in Gelsemium elegans may be useful for its quality control. The present analytical method combined with the developed Gelsemium database was shown to be a useful tool for investigating the chemical components of Gelsemium products. © 2017 Elsevier B.V.
语种：
英文
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三种钩吻粗提物对HepG2细胞毒性作用研究
作者：
代佳娣;李玉娟;张卓亿;孙志良;刘兆颖
作者机构：
[代佳娣; 李玉娟; 张卓亿; 孙志良; 刘兆颖] 湖南农业大学动物医学院
会议名称：
中国毒理学会兽医毒理学委员会与中国畜牧兽医学会兽医食品卫生学分会联合学术研讨会暨中国毒理学会兽医毒理学委员会第5次全国会员代表大会
会议时间：
2017-09-22
会议地点：
中国北京
关键词：
钩吻;人肝癌细胞;代谢;细胞凋亡
摘要：
【目的】本试验通过比较三种不同的钩吻粗提物(钩吻醇提物、水提物以及酸水提取物)对体外培养的人肝癌细胞(HepG2)的毒性作用,并通过加入肝S9与NADPH和细胞的共孵育来进一步探讨三种粗提物对细胞毒性的影响,以期研究三种钩吻提取物对体外培养的细胞毒性作用的差异,以及其是否存在代谢活化或者代谢失活。为今后更深入地研究钩吻的毒性与代谢奠定基础。【方法】用不同浓度的三种钩吻粗提物孵育HepG2细胞24h
语种：
中文
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ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice
作者：
Xiao, Hong-Bo* ;Wang, Ji-Ying;Sun, Zhi-Liang
期刊：
Experimental and Molecular Pathology ,2017年103(3):242-248 ISSN：0014-4800
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Wang, Ji-Ying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Wang, Ji-Ying] Huai Hua Vocat & Tech Coll, Dept Anim Sci & Technol, Huaihua 418000, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
*Angiopoietin-like protein 3;*Hyperlipidemia;*Lipoprotein lipase;*Mastitis;*Mice
摘要：
Previous investigations have shown that inflammation induces changes in lipid and lipoprotein metabolism, and increased expression of angiopoietin-like protein 3 (ANGPTL3) contributes to the development of dyslipidemia. Here we investigated whether there is a correlation between increased ANGPTL3 expression and dyslipidemia in mastitis mice. Thirty mice were divided into two groups: control group and Staphylococcus aureus (S. aureus)–induced mastitis mice group. Changes in the levels of blood lipids [total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C)]; activity of myeloperoxidase (MPO); concentrations of plasma inflammation biomarkers [interferon-γ (IFNγ), tumor necrosis factor α (TNFα), and interleukin-1α (IL-1α)]; concentration of plasma ANGPTL3 protein; lipoprotein lipase (LPL) activities in postheparin plasma; expressions of hepatic N-acetylgalactosaminyltransferase 2 (GALNT2), hepatic ANGPTL3 and adipose LPL were determined. The major results indicated specific pathological mammary tissue changes, elevated MPO activity, reduced GALNT2 mRNA expression, elevated ANGPTL3 mRNA and protein expression and reduced LPL mRNA and protein expression. In plasma samples the S.aureus infused mice displayed elevated ANGPTL3 protein concentration, TG, TC and LDL-C levels, and reduced postheparin LPL activities and HDL-C level. The data suggests that ANGPTL3 is part of the machinery causing dyslipidemia majorily via LPL inhibition in mastitis mice. © 2017 Elsevier Inc.
语种：
英文
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