不同菌株接入盐渍辣椒汁和干辣椒混合体系发酵风味物质的比较
作者：
方俊;陈怡;蒋立文;刘洋;李跑;...
期刊：
中国酿造 ,2021年40(01):111-117 ISSN：0254-5071
作者机构：
[蒋立文; 邓放明; 刘洋; 李跑] 湖南农业大学食品科学技术学院;食品科学与生物技术湖南省重点实验室;[覃业优] 湖南坛坛香食品科技有限公司;[陈怡; 方俊] 湖南农业大学食品科学技术学院<&wdkj&>食品科学与生物技术湖南省重点实验室
关键词：
乳酸菌;盐渍辣椒汁;干辣椒;发酵;风味物质
摘要：
选取2株植物乳杆菌（Lactobacillus plantarum）（1.3919、1.555）、2株产香鲁氏结合酵母菌（Zygosaccharomyces rouxii）（2.1913、2.371）和1株凝结芽孢杆菌（Bacillus coagulans）1.3220分别接入以盐渍辣椒汁与干辣椒混合体系（干辣椒∶盐渍辣椒汁（盐度10%）=1∶3（g∶g））中进行发酵30 d后,通过气质谱联用（GC-MS）法测定其挥发性风味物质的组成,采用Up Set软件及主成分分析（PCA）研究其风味物质的共性及差异。结果表明,6个样品中共检测出挥发性物质100种,其中醇类（19种）、酯类（41种）、酸类（10种）、酚类（8种）、酮类（3种）、其他（19种）。接菌发酵有助于产品的风味的形成,6个样品中共有的挥发性物质14种,以酯类物质为主。样品PZ-2.1913与PL-1.3919、样品PL-1.555与PZ-2.371中挥发性物质种类和含量相似。结果表明菌株1.3919更适合接入盐渍辣椒汁与发酵干辣椒混合体系发酵。
语种：
中文
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淡豆豉的生理活性和生产工艺研究进展
作者：
陈怡;刘洋;覃业优;蒋立文;李跑;...
期刊：
食品安全质量检测学报 ,2020年11(17):5948-5954 ISSN：2095-0381
作者机构：
湖南农业大学食品科学技术学院;[陈怡; 刘洋; 蒋立文] 食品科学与生物技术湖南省重点实验室;[覃业优] 湖南坛坛香食品科技有限公司;[李跑; 廖卢艳] 湖南农业大学
关键词：
淡豆豉;生理活性;炮制工艺
摘要：
淡豆豉是一种药食同源的食品原料,具有"解表除烦"、抗氧化、抗菌、降血糖、溶血栓等多种药理活性。本文对目前淡豆豉的生理活性研究现状及其物质基础进行了总结;并且通过结合淡豆豉发酵过程中微生物菌群及活性成分的变化规律,对主要有效成分如大豆异黄酮、豆豉纤溶酶、γ-氨基丁酸等的合成调控进行初步分析,并进一步总结了淡豆豉的工艺优化现状和不足之处,对淡豆豉的产业化生产和高值化利用提出合理建议与展望,以期为淡豆豉的产业化提供提供科学的指导。
语种：
中文
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浏阳豆豉发酵中高产酶活菌株的分离鉴定及酶活性分析
作者：
陈怡;刘洋;蒋立文;李跑;伟明;...
期刊：
中国酿造 ,2020年39(8):37-41 ISSN：0254-5071
作者机构：
湖南农业大学食品科学技术学院;[陈怡; 刘洋; 蒋立文] 食品科学与生物技术湖南省重点实验室;[伟明] 溢品鲜调味食品厂;[覃业优] 湖南坛坛香食品科技有限公司;[李跑] 湖南农业大学
关键词：
溜曲霉;蛋白酶活力;脂肪酶活力;纤维素酶活力
摘要：
从浏阳豆豉发酵过程中分离产高酶活菌株,通过形态观察结合分子生物学技术进行鉴定,并对其产蛋白酶、脂肪酶及纤维素酶的活性进行分析。结果表明,分离得到3株菌（编号为000、5132、621）均被鉴定为溜曲霉菌（Aspergillus tamarri）。3株菌的蛋白酶、脂肪酶及纤维素酶的活性测定结果表明,菌株621蛋白酶活性最强,为（207.98±3.20）U/m L;菌株5132的纤维素酶活性最强,为（3.40±1.40）U/m L;菌株000的脂肪酶活性最高,为（90.7±0.64）U/m L。
语种：
中文
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不同类型豆豉抗氧化活性差异与影响因素分析
作者：
陈怡;陆敏捷;刘洋;蒋立文;李跑;...
期刊：
食品与发酵工业 ,2020年46(13):36-41 ISSN：0253-990X
作者机构：
湖南农业大学 食品科学技术学院,湖南 长沙,410128;食品科学与生物技术湖南省重点实验室(湖南农业大学),湖南 长沙,410128;[李跑; 陈怡; 廖卢艳; 刘洋; 陆敏捷; 蒋立文] 湖南农业大学
关键词：
抗氧化活性;大豆异黄酮;豆豉;多酚
摘要：
为明确不同类型豆豉抗氧化活性的差异以及影响因素,测定了细菌型、曲霉型和毛霉型豆豉的羟基自由基( ·OH)清除率、总抗氧化能力以评价其抗氧化能力;通过测定pH、蛋白含量、水解度、多酚含量及大豆异黄酮含量,分析了不同类型豆豉抗氧化活性差异的原因。结果表明,霉型黄姚豆豉的总抗氧化能力最强,为( 0.6 ± 0.01) mmol /L,曲霉型稻香元豆豉的·OH清除率最强,为( 65.3 ± 0.62) %,而细菌型滨梨纳豆的抗氧化能力最弱,分别为( 0.1 ± 0.04) mmol /L、( 5.4 ± 0.04) %。同时霉型豆豉总多酚含量及苷元型异黄酮含量明显高于细菌型,其中分别以毛霉型杨晋记豆豉( 71.9 ± 0.91) g /100 g和黄姚豆豉( 2 925.4 ± 12.32) mg /kg最高。通过相关性分析发现,多酚和苷元型异黄酮含量分别与·OH清除率及总抗氧化能力显著相关( P < 0.05) 。
语种：
中文
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基于高通量测序分析不同浏阳豆豉中真菌的多样性
作者：
陈怡;刘洋;蒋立文;李跑;廖卢艳
期刊：
中国酿造 ,2020年39(11):66-70 ISSN：0254-5071
作者机构：
湖南农业大学食品科学技术学院;[陈怡; 蒋立文] 食品科学与生物技术湖南省重点实验室;[李跑; 廖卢艳; 刘洋] 湖南农业大学
关键词：
浏阳豆豉;ITS高通量测序;真菌
摘要：
为比较不同品牌的浏阳豆豉中真菌分布情况,采用ITS高通量测序对4种品牌的浏阳豆豉(LJ,TPQ,TMS,YPX)中真菌组成进行检测,并通过韦恩图、热图和主坐标分析(PCoA)对其结果进行分析.结果表明,4种豆豉存在共有的146个操作分类单元(OTUs),TPQ豆豉样品的真菌相对丰度最大,LJ豆豉样品最低.4种浏阳豆豉均以子囊菌门(Ascomycota)、毛霉亚门(Mucoromycota)为主要菌群门类,LJ和YPX豆豉样品中均以曲霉属(Aspergillus)为主要菌属,其中LJ豆豉中曲霉属相对丰度最高(91.4％),TPQ豆豉样品中小囊菌属(Microascus)相对丰度最高(49.1％).热图聚类结果反映出各品牌浏阳豆豉中具有其特有的菌属,横梗霉属(Lichtheimia)主要存在于TMS与LJ豆豉样品中,嗜热菌属(Thermomyces)在TPQ豆豉样品中含量最高,曲霉属(Aspergillus)、单端孢属(Trrichothecium)和根霉属(Rizopus)均在LJ中含量较高,木霉属(Trichoderma)、单端孢属(Trrichothecium)、青霉属(Penicillium)、毛壳菌属(Chaetomium)在YPX含量最高.主坐标分析(PCoA)结果说明YPX豆豉样品菌群与LJ豆豉样品相似,而与TMS和TPQ豆豉样品相差较大.
语种：
中文
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浏阳豆豉发酵过程中抗氧化成分及活性变化研究
作者：
陈怡;刘洋;蒋立文;李跑;廖卢艳
期刊：
食品工业科技 ,2020年41(19):273-278 ISSN：1002-0306
作者机构：
湖南农业大学食品科学技术学院;[陈怡] 食品科学与生物技术湖南省重点实验室;[李跑; 廖卢艳; 刘洋; 蒋立文] 湖南农业大学
关键词：
浏阳豆豉;大豆异黄酮;总黄酮;总多酚;抗氧化活性
摘要：
通过测定大豆异黄酮、总黄酮、总多酚含量的变化,结合发酵过程中浏阳豆豉的抗氧化活性的变化,来探讨浏阳豆豉抗氧化活性变化的机理。结果表明:虽然总黄酮与总大豆异黄酮的含量逐步减少,但发酵初期原料中大豆异黄酮主要为糖苷型(占85.75%),而发酵末期则主要为更具活性的苷元型大豆异黄酮(占81.41%),且总多酚经发酵从2.78 mg/g提升至5.76 mg/g,发酵最后一天(L20)豆豉的总抗氧化能力、DPPH·、·OH、O~-_2·清除率分别增加至1088.91 U/g、93.67%、90.24%、88.38%,此外相关性结果表明苷元型大豆异黄酮要比总多酚对豆豉抗氧化活性的影响更大。实验证明发酵有助于大豆中总多酚的增加以及糖苷型大豆异黄酮向苷元型大豆异黄酮转换,这是提高豆豉抗氧化能力及功能性的关键因素之一。
语种：
中文
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以腌渍辣椒水作为发酵剂发酵干辣椒的微生物筛选
作者：
陈怡;方俊;覃业优;蒋立文;刘洋;...
期刊：
中国酿造 ,2020年39(10):109-113 ISSN：0254-5071
作者机构：
湖南农业大学食品科学技术学院;[陈怡; 方俊] 食品科学与生物技术湖南省重点实验室;[覃业优] 湖南坛坛香食品科技有限公司;[李跑; 廖卢艳; 周辉; 刘洋; 王蓉蓉; 蒋立文] 湖南农业大学
关键词：
干辣椒;植物乳杆菌;鲁氏结合酵母菌;凝结芽孢杆菌;盐渍辣椒汁
摘要：
该研究选择盐渍辣椒汁配以一定比例干辣椒混合发酵,探讨盐渍辣椒汁的利用新途径.选取2株植物乳杆菌(Lactobacillus plantarum) 1.3919、1.555、2株产香鲁氏接合酵母菌(Zygosaccharomyces rouxii) 2.1913、2.371和1株凝结芽孢杆菌(Bacillus coagulans) 1.3220作为原始菌株,通过对其生长曲线、产酸能力、耐盐能力的测试最终筛选出植物乳杆菌1.3919和鲁氏接合酵母菌2.371为最适发酵菌,其正常条件下最大OD850nm值分别为5.41、5.40;而产酸能力则分别为1.98％、0.65％;上述两株菌在盐度为10％条件下有较强耐受力,其最大OD850nm值均达到5.4.将其分别接入以盐渍辣椒汁∶干辣椒(3∶1)体系中进行发酵,各组产酸能力均在15 d时达到最大,菌株1.3919、2.371的酸度分别为1.23％、1.03％.
语种：
中文
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Non-muscle Myosin II: Role in Microbial Infection and Its Potential as a Therapeutic Target
作者：
Tan, Lei;Yuan, Xiaomin;Liu, Yisong;Cai, Xiong;Guo, Shiyin;...
期刊：
Frontiers in Microbiology ,2019年10(MAR):401 ISSN：1664-302X
通讯作者：
Wang, AB;Guo, SY
作者机构：
[Liu, Yisong; Wang, Aibing; Wang, AB; Tan, Lei; Yuan, Xiaomin] Hunan Agr Univ, Coll Vet Med, Res & Dev Ctr Anim Reverse Vaccinol Hunan Prov, Hunan Prov Key Lab Prot Engn Anim Vaccines, Changsha, Hunan, Peoples R China.;[Cai, Xiong] Hunan Univ Chinese Med, Inst Innovat & Appl Res Chinese Med, Changsha, Hunan, Peoples R China.;[Guo, Shiyin] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Wang, AB ; Guo, SY ] H;Hunan Agr Univ, Coll Vet Med, Res & Dev Ctr Anim Reverse Vaccinol Hunan Prov, Hunan Prov Key Lab Prot Engn Anim Vaccines, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
关键词：
co-factors/receptors;mammalian cells;microbial-triggered discords;non-muscle myosin II;regulators;regulatory pathways
摘要：
Currently, the major measures of preventing and controlling microbial infection are vaccinations and drugs. However, the appearance of drug resistance microbial mounts is main obstacle in current anti-microbial therapy. One of the most ubiquitous actin-binding proteins, non-muscle myosin II (NM II) plays a crucial role in a wide range of cellular physiological activities in mammals, including cell adhesion, migration, and division. Nowadays, growing evidence indicates that aberrant expression or activity of NM II can be detected in many diseases caused by microbes, including viruses and bacteria. Furthermore, an important role for NM II in the infection of some microbes is verified. Importantly, modulating the expression of NM II with small hairpin RNA (shRNA) or the activity of it by inhibitors can affect microbial-triggered phenotypes. Therefore, NM II holds the promise to be a potential target for inhibiting the infection of microbes and even treating microbial-triggered discords. In spite of these, a comprehensive view on the functions of NM II in microbial infection and the regulators which have an impact on the roles of NM II in this context, is still lacking. In this review, we summarize our current knowledge on the roles of NM II in microbial-triggered discords and provide broad insights into its regulators. In addition, the existing challenge of investigating the multiple roles of NM II in microbial infection and developing NM II inhibitors for treating these microbial-triggered discords, are also discussed.
语种：
英文
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Acetylome profiling reveals extensive involvement of lysine acetylation in the conversion of muscle to meat
作者：
Jiang, Shengwang;Liu, Yisong;Shen, Zhenglei;Zhou, Bing;Shen, Qingwu W.*
期刊：
Journal of Proteomics ,2019年205:103412 ISSN：1874-3919
通讯作者：
Shen, Qingwu W.
作者机构：
[Jiang, Shengwang; Shen, Qingwu W.; Liu, Yisong; Zhou, Bing] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shen, Zhenglei] Nanya Middle Sch, Changsha 410129, Hunan, Peoples R China.
通讯机构：
[Shen, Qingwu W.] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
calcium;calcium binding protein;cytoplasm protein;enolase;glucose;glyceraldehyde 3 phosphate dehydrogenase;lysine;mitochondrial protein;muscle protein;myosin heavy chain;phenylalanine;pyruvate kinase;tyrosine;acyltransferase;lysine;muscle protein;proteome;acetylation;animal tissue;apoptosis;Article;calcium signaling;cell stress;cellular distribution;deacetylation;endoplasmic reticulum;food quality;glucose metabolism;glycogenolysis;glycolysis;immunoprecipitation;meat;muscle;muscle contraction;nonhuman;pH;pig;postmortem change;priority journal;protein degradation;protein protein interaction;rigor mortis;Western blotting;acetylation;animal;metabolism;pathology;physiology;procedures;protein processing;proteomics;veterinary medicine;Acetylation;Acetyltransferases;Animals;Lysine;Muscle Contraction;Muscle Proteins;Muscles;Pork Meat;Postmortem Changes;Protein Processing, Post-Translational;Proteome;Proteomics;Rigor Mortis;Swine
摘要：
Protein lysine acetylation is an post-translational modification that regulates gene expression, metabolism, cell signaling, and diseases, but its implication in the postmortem (PM) meat quality development is basically unclear. In the present study, a quantitative proteomic analysis was conducted to profile acetylome in porcine muscle within 24 h PM. In total 595 acetylation sites assigned to 163 proteins were identified in porcine muscle, of which 460 sites distributing to 110 proteins significantly changed in acetylation levels in the conversion of muscle to meat. The dynamic acetylation/deacetylaion of muscle proteins was closely associated with critical chemical-biophysical changes in PM muscle. Bioinformatic analysis revealed that protein lysine acetylation likely regulated postmortem meat quality development by regulating glycolysis and muscle pH, cell stress reponse and apoptosis, muscle contraction and rigor mortis, calcium signaling and proteolysis, IMP synthesis and meat flavor development, and even the stability of pigment proteins and meat color. This study provided the first overview of protein lysine acetylation in PM muscle and revealed its significance in the conversion of muscle to meat. Future exploration of the exact role of protein lysine acetylation at specific sites will further our understanding regarding the underlying mechanisms and be helpful for meat quality control. Significance: This is the first analysis of acetylome in farm animal and postmortem muscle. Our data showed that the dynamic acetylation/deacetylation of muscle proteins was closely related to the postmortem changes of muscle that affect the final quality of raw meat. Proteins related to glucose metabolism and muscle contraction were the two largest clusters of acetylproteins identified in postmortem porcine muscle. Networks of acetylproteins involved in apoptosis, calcium signaling and IMP synthesis were identified in postmortem porcine muscle at the same time. Our results revealed that protein lysine acetylation regulated the conversion of muscle to meat. It likely regulated meat quality development by regulating postmortem glycolysis, mitochondrion initiated cell apoptosis, calcium signaling, rigor mortis, meat flavor compound sysnthesis and meat tenderization. Our study broadened our understanding of the biochemistry regulating the postmortem conversion of muscle to meat and final meat quality development, which may be helpful for future meat quality control. © 2019
语种：
英文
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Phosphorylation of nucleolin is indispensable to upregulate miR-21 and inhibit apoptosis in cardiomyocytes
作者：
Tong, Zhongyi;Tang, Yuting;Jiang, Bimei* ;Wu, Yanyang;Liu, Yanjuan;...
期刊：
JOURNAL OF CELLULAR PHYSIOLOGY ,2019年234(4):4044-4053 ISSN：0021-9541
通讯作者：
Jiang, Bimei
作者机构：
[Xiao, Xianzhong; Li, Yuanbin; Tong, Zhongyi; Liu, Yanjuan; Jiang, Bimei; Tang, Yuting] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha, Hunan, Peoples R China.;[Tong, Zhongyi] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha, Hunan, Peoples R China.;[Wu, Yanyang] Hunan Agr Univ, Food Sci & Technol Coll, Dept Microbiol, Changsha, Hunan, Peoples R China.
通讯机构：
[Jiang, Bimei] C;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha, Hunan, Peoples R China.
关键词：
alanine;hydrogen peroxide;microRNA 21;nucleolin;threonine;Ago2 protein, rat;argonaute protein;hydrogen peroxide;microRNA;MIRN21 microRNA, mouse;mirn21 microRNA, rat;nucleolin;phosphoprotein;RNA binding protein;animal cell culture;animal experiment;animal model;apoptosis;Article;cardiac muscle cell;cardiac myoblast;cell protection;H9c2(2-1) cell line;in vitro study;in vivo study;male;myocardial ischemia reperfusion injury;nonhuman;oxidative stress;priority journal;protein expression;protein glycosylation;protein phosphorylation;protein processing;rat;site directed mutagenesis;animal;Bagg albino mouse;cardiac muscle cell;cell line;disease model;drug effect;genetics;metabolism;mutation;pathology;phosphorylation;signal transduction;upregulation;Animals;Apoptosis;Argonaute Proteins;Cell Line;Disease Models, Animal;Hydrogen Peroxide;Male;Mice, Inbred BALB C;MicroRNAs;Mutation;Myocardial Reperfusion Injury;Myocytes, Cardiac;Oxidative Stress;Phosphoproteins;Phosphorylation;Rats;RNA-Binding Proteins;Signal Transduction;Up-Regulation
摘要：
Nucleolin is a multifunctional phosphoprotein and is involved in protecting from myocardial ischemia/reperfusion (I/R) injury. The function of nucleolin is regulated by posttranslational modifications, including phosphorylation and glycosylation. To study whether phosphorylation of nucleolin (P-nucleolin) was involved in the protection from myocardial I/R injury. We investigated the expression pattern of P-nucleolin (Thr-76 and 84) in hearts subjected to I/R injury, or rat cardiac myoblast cells (H9C2) subjected to hydrogen peroxide (H 2 O 2 ). The results showed that the expression of P-nucleolin and the ratio of P-nucleolin/nucleolin were significantly increased both in vivo and in vitro. Mutant nucleolin was obtained by site directed mutagenesis in vitro: threonine at 76 and 84 was replaced by alanine, and we found that the protective effect of nucleolin on apoptosis induced by oxidative stress was dependent on its phosphorylation at 76 and 84 in H9C2 cells. Furthermore, the cardio-protective roles of P-nucleolin (Thr-76 and 84) in H9C2 cardiomyocytes, were attributable to the upregulation of microRNA (miR)-21. Further analysis found that P-nucleolin (Thr-76 and 84) could bind to miR-21, and P-nucleolin colocalized with argonaute 2 (Ago2) in cytoplasm and could interact with Ago2 in a RNA-independent manner under cell oxidative stress. The current study revealed that P-nucleolin (Thr-76 and 84) increased in I/R injury myocardium, P-nucleolin was indispensable to upregulate miR-21 and inhibited apoptosis induced by H 2 O 2 in H9C2 cardiomyocytes. These findings provided new insight into the molecular mechanisms of nucleolin in myocardial I/R injury and oxidative stress cells. © 2018 Wiley Periodicals, Inc.
语种：
英文
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Proteomic analysis reveals that lysine acetylation mediates the effect of antemortem stress on postmortem meat quality development
作者：
Zhou, Bing;Shen, Zhenglei;Liu, Yisong;Wang, Chengtao* ;Shen, Qingwu W.*
期刊：
Food Chemistry ,2019年293:396-407 ISSN：0308-8146
通讯作者：
Wang, Chengtao;Shen, Qingwu W.
作者机构：
[Shen, Qingwu W.; Zhou, Bing; Wang, Chengtao] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 11 Fucheng Rd, Beijing 100048, Peoples R China.;[Shen, Qingwu W.; Liu, Yisong; Zhou, Bing] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shen, Zhenglei] Nanya Middle Sch, Changsha 410129, Hunan, Peoples R China.
通讯机构：
[Wang, Chengtao] B;[Shen, Qingwu W.] H;Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 11 Fucheng Rd, Beijing 100048, Peoples R China.;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Antemortem stress;Glycolysis;Meat quality;Protein lysine acetylation;Proteomics
摘要：
To explore the involvement of protein lysine acetylation in the conversion of muscle to meat, a quantitative analysis of the acetylome in postmortem porcine muscle with or without antemortem stress was conducted. In total, 771 acetylpeptides containing 681 lysine acetylation sites mapping to 176 acetylproteins were identified. Acetylproteins were enriched in muscle contraction, carbohydrate metabolism, cell apoptosis and calcium signaling. Bioinformatic analysis suggests that preslaughter handling may be associated with glycolysis in postmortem muscle and the overall meat quality, via acetylation of multiple enzymes of glycogenolysis/glycolysis, regulate rigor mortis via acetylation of contractile, ATP production and calcium signaling-related proteins, and regulate stress response, cell apoptosis and meat tenderization via regulating the functions of heat shock proteins and permeability transition pore complex. This study provides the first overview of the acetylome in postmortem muscle as affected by preslaughter handling and broadens knowledge of the biochemistry regulating meat quality development.
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Detection of Edible Plant Oil Adulteration by Triacylglycerol Profiles Using an Atmospheric Pressure Chemical Ionization Source and MS3 Ion Trap Mass Spectrometry
作者：
Wang, Xiupin;Li, Peiwu* ;Liu, Xia;Liu, Youqian;Zhang, Qi;...
期刊：
European Journal of Lipid Science and Technology ,2019年121(12):1900029-1-1900029-8 ISSN：1438-7697
通讯作者：
Li, Peiwu
作者机构：
[Wang, Xiupin; Li, Peiwu; Liu, Youqian; Zhang, Qi; Zhang, Liangxiao] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China;[Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Natl Reference Lab Agr Testing Mycotoxin, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Liu, Youqian; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Hubei, Peoples R China
通讯机构：
[Li, Peiwu] C;[Li, Peiwu] M;[Li, Peiwu] N;Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Hubei, Peoples R China. Natl Reference Lab Agr Testing Mycotoxin, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Hubei, Peoples R China.
关键词：
adulterated edible plant oil;multivariate statistical methods;RPLC-APCI-IT-MS;triacylglycerol profiles
摘要：
An adulteration of high-price oil has been an important concern in agri-food fraud problems. Triacylglycerols are the main components of edible plant oils, which play an important role as target for adulteration detections. The objective is to evaluate the utility of triacylglycerol profiles in the detection of adulteration in high priced oils. An effective method is established to detect adulteration of high priced oils based on triacylglycerol profiles and chemometrics. Triacylglycerol profiles of edible oils are analyzed by an APCI-MS3-IT-MS. All triacylglycerol compounds are quantified by normalization of the chromatographic peak area in selected reaction monitoring (SRM) mode based on MS3 fragment ion pairs, which are used to eliminate interference from the isobaric species of triacylglycerol. The results of the hierarchical cluster analysis (HCA) and random forest (RF) classification algorithm indicated that peanut, soybean, sesame, sunflower seed, and linseed oils are completely classified into five groups based on their triacylglycerol profiles. Finally, a recursive support vector machine (R-SVM) discriminant model is established, which can successfully identify adulteration of high priced oil with cheaper edible oils at a concentration of as low as 4% with an accuracy of 93.7%. Practical Applications: The triacylglycerol profiles of edible plant oils are analyzed by RPLC-APCI-IT-MS. The SRM scan mode based on MS3 fragment ion pairs can eliminate interference of triacylglycerol isomers. The discriminant model for adulterated high-price oils is established by R-SVM. HCA, and RF can classify the five kinds of edible plant oil. The triacylglycerol of five edible plant oils is analyzed by RPLC-APCI-MS3-IT-MS. The triacylglycerol data matrix is submitted using unsupervised (HCA) and supervised (RF) chemometric methods to classify five kinds of edible plant oil. A discriminant model established with the R-SVM can be used to identify adulterated linseed oil samples (>= 4%) with an accuracy of 93.7%.
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Surface plasmon resonance immunosensor for fast, highly sensitive, and in situ detection of the magnetic nanoparticles-enriched Salmonella enteritidis
作者：
Liu, Xia* ;Hu, Yuxin;Zheng, Shu;Liu, Yan;He, Zao;...
期刊：
Sensors and Actuators B-Chemical ,2016年230:191-198 ISSN：0925-4005
通讯作者：
Liu, Xia
作者机构：
[Liu, Yan; He, Zao; Hu, Yuxin; Luo, Fang; Liu, Xia; Zheng, Shu] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Surface plasmon resonance immunosensor;Salmonella enteritidis detection;Immune magnetic nanoparticles separation;Sandwich assay
摘要：
A highly sensitive surface plasmon resonance (SPR) immunosensor combining antibody-functionalized Fe<inf>3</inf>O<inf>4</inf>magnetic nanoparticles (immunoMNPs) separation is reported for rapid detection of Salmonella enteritidis (S. enteritidis) based on the sandwich immunoassay. Here, immunoMNPs simultaneously served not only as "vehicles" for rapid delivery of analyte from a sample to the sensor surface, but also as labels increasing the measured refractive index changes associated with the binding of analyte. S. enteritidis can be detected at the concentration as low as 14 cfu/mL with a good linear signal range at 1.4 ×10<sup>1</sup>-1.4 ×10<sup>9</sup>cfu/mL. Moreover, the use of immunoMNPs gives 4 orders of magnitude improvement in the sensitivity toward S. enteritidis compared with regular SPR immunosensor with direct detection format. The selectivity of proposed SPR approach was examined with Escherichia coli K12 ER2738 and Lactobacillus LJ-3. The recovery of 92.76-113.25% was obtained in eggshell. The developed SPR immunosensor has potential to provide a simple, low-cost and sensitive method for in situ detection of foodborne pathogens. ©2016 Published by Elsevier B.V.
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Different actions of salt and pyrophosphate on protein extraction from myofibrils reveal the mechanism controlling myosin dissociation
作者：
Shen, Qingwu W.;Swartz, Darl R.;Wang, Zhenyu;Liu, Yue;Gao, Yuan;...
期刊：
Journal of the Science of Food and Agriculture ,2016年96(6):2033-2039 ISSN：0022-5142
通讯作者：
Zhang, Dequan
作者机构：
[Shen, Qingwu W.] Chinese Acad Agr Sci, Key Lab Agroprod Proc, Inst Food Sci & Technol, Minist Agr, Beijing 100193, Peoples R China.;[Shen, Qingwu W.; Gao, Yuan; Zhang, Dequan; Liu, Yue; Wang, Zhenyu] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Swartz, Darl R.] Delaware Valley Coll, Doylestown, PA 18901 USA.;[Zhang, Dequan] Chinese Acad Agr Sci, Inst Food Sci & Technol, 1 NongDa South Rd, Beijing 100193, Peoples R China.
通讯机构：
[Zhang, Dequan] C;Chinese Acad Agr Sci, Inst Food Sci & Technol, 1 NongDa South Rd, Beijing 100193, Peoples R China.
关键词：
muscle protein;myosin;pyrophosphoric acid derivative;sodium chloride;animal;bovine;chemistry;muscle fibril;Animals;Cattle;Diphosphates;Muscle Proteins;Myofibrils;Myosins;Sodium Chloride
摘要：
Background: Myosin is the major functional protein in muscle foods for water retention, protein binding/gelation and fat holding/emulsification. To maximize its functionality, myosin needs to be released from thick filaments. Understanding of the mechanism controlling myosin extraction will help improve quality traits of meat products. Results: The data obtained show that actomyosin binding is the rate-limiting constraint for myosin release in rigor condition. Magnesium pyrophosphate (MgPPi) increased myosin extraction by weakening actomyosin interaction and maximized myosin extraction at 0.4 mol L-1 NaCl, which was not attained at 1.0 mol L-1 NaCl in the absence of PPi. Interaction between myosin rod domains is another critical constraint for myosin extraction, which is, rather than PPi, salt dependent. Further, our data suggest that MyBP-C (myosin binding protein C) and M-line might not be of significance in the process of NaCl-induced myosin extraction, though further study was needed. Conclusion: Our study provides new insight into the mechanism that controls myosin extraction from intact sarcomere, which could be applied to maximize myosin function and to improve meat quality in practice. © 2016 Society of Chemical Industry.
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英文
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Role of the ubiquitin-proteasome pathway on proteolytic activity in postmortem proteolysis and tenderisation of sheep skeletal muscle
作者：
Liu, Yue;Du, Manting;Li, Xin;Chen, Li;Shen, Qingwu;...
期刊：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ,2016年51(11):2353-2359 ISSN：0950-5423
通讯作者：
Zhang, Dequan
作者机构：
[Du, Manting; Zhang, Dequan; Liu, Yue; Chen, Li; Li, Xin] Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, Inst Food Sci & Technol, Beijing 100193, Peoples R China.;[Tian, Jianwen; Liu, Yue] Ningxia Univ, Sch Agr, Ningxia 750021, Peoples R China.;[Shen, Qingwu] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhang, Dequan] C;Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, Inst Food Sci & Technol, Beijing 100193, Peoples R China.
关键词：
Proteasome;sheep;skeletal muscle;tenderisation;ubiquitin
摘要：
The objective of this study was to investigate the effects of ubiquitin-proteasome pathway on meat tenderisation. The sheep muscle longissimus lumborum was injected with or without PYR-41 (inhibitor of ubiquitination) or MG-132 (inhibitor of proteasome). Muscle samples were collected at 6, 15, 24 and 48h after injection. Myofibrillar protein degradation, muscle ultrastructure and sarcomere length were determined. Results showed that inhibition of proteasome or ubiquitination affected sarcomere length at 48h after treatments. Destruction of muscle ultrastructure in both treatments was reduced when compared to control. Inhibition of proteasome produced different fragments of myofibrillar proteins in comparison with control at 48h. In conclusion, ubiquitin-proteasome plays a role in postmortem proteolysis and might contribute to meat tenderisation. © 2016 Institute of Food Science and Technology
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Electrochemical Sensor based on Imprinted Sol-Gel Polymer on Au NPs-MWCNTs-CS Modified Electrode for the Determination of Acrylamide
作者：
Liu, Xia* ;Mao, Lu-Gang;Wang, Yuan-Liang;Shi, Xing-Bo;Liu, Yan;...
期刊：
Food Analytical Methods ,2016年9(1):114-121 ISSN：1936-9751
通讯作者：
Liu, Xia
作者机构：
[Shi, Xing-Bo; Liu, Yan; Wang, Yuan-Liang; He, Zao; Mao, Lu-Gang; Yang, Yang; Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Electrochemical sensor;Acrylamide;Gold nanoparticles;Chitosan;Multiwalled carbon nanotubes;Sol-gel molecularly imprinted
摘要：
A sensitive molecularly imprinted electrochemical sensor was successfully constructed for the detection of acrylamide (AM). It is based on a glassy carbon electrode modified with a composites prepared from gold nanoparticles, multiwalled carbon nanotubes, and chitosan along with a sol-gel-based molecularly imprinted polymer (MIP) film. The latter was prepared using AM as the template molecule, 3-aminopropyltrimethoxysilane as the functional monomer, and tetraethoxysilane as the cross-linker. The MIP sensor showed a linear current response to the target AM concentration in the range from 0.05 to 5μgmL−1 at a working voltage of 0–0.4V with a lower detection limit of 0.028μgmL−1 (S/N = 3). It was successfully applied to the detection of AM in potato chips. HPLC analysis was also conducted to detect AM in the same samples to demonstrate the applicability of the electrochemical MIP sensor. © 2015, Springer Science+Business Media New York.
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英文
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MiR-21 protected cardiomyocytes against doxorubicin-induced apoptosis by targeting BTG2
作者：
Tong, Zhongyi;Jiang, Bimei* ;Wu, Yanyang;Liu, Yanjuan;Li, Yuanbin;...
期刊：
International Journal of Molecular Sciences ,2015年16(7):14511-14525 ISSN：1661-6596
通讯作者：
Jiang, Bimei
作者机构：
[Tong, Zhongyi] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha 410000, Hunan, Peoples R China.;[Xiao, Xianzhong; Li, Yuanbin; Tong, Zhongyi; Liu, Yanjuan; Lv, Qinglan; Jiang, Bimei; Gao, Min; Jiang, Yu] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410000, Hunan, Peoples R China.;[Wu, Yanyang] Hunan Agr Univ, Food Sci & Technol Coll, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Jiang, Bimei] C;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410000, Hunan, Peoples R China.
关键词：
doxorubicin;lactate dehydrogenase;microRNA 21;antineoplastic agent;BTG2 protein, rat;doxorubicin;immediate early protein;microRNA;mirn21 microRNA, rat;tumor suppressor protein;animal cell;animal experiment;animal model;Article;cardiotoxicity;cell proliferation;cell viability;computer model;controlled study;flow cytometry;heart function;heart left ventricle enddiastolic pressure;heart muscle cell;male;mouse;nonhuman;polyacrylamide gel electrophoresis;protein expression;real time polymerase chain reaction;Western blotting;animal;apoptosis;cardiac muscle cell;cell culture;cell line;drug effects;genetics;metabolism;rat;Mus;Rattus;Animals;Antineoplastic Agents;Apoptosis;Cell Line;Cells, Cultured;Doxorubicin;Immediate-Early Proteins;Mice;MicroRNAs;Myocytes, Cardiac;Rats;Tumor Suppressor Proteins
摘要：
Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of antineoplastic activities. However, it causes cardiac cytotoxicity, and this limits its clinical applications. MicroRNA-21 (miR-21) plays a vital role in regulating cell proliferation and apoptosis. While miR-21 is preferentially expressed in adult cardiomyocytes and involved in cardiac development and heart disease, little is known regarding its biological functions in responding to DOX-induced cardiac cytotoxicity. In this study, the effects of DOX on mouse cardiac function and the expression of miR-21 were examined in both mouse heart tissues and rat H9C2 cardiomyocytes. The results showed that the cardiac functions were more aggravated in chronic DOX injury mice compared with acute DOX-injury mice; DOX treatment significantly increased miR-21 expression in both mouse heart tissue and H9C2 cells. Over-expression of miR-21 attenuated DOX-induced apoptosis in cardiamyocytes whereas knocking down its expression increased DOX-induced apoptosis. These gain- and loss- of function experiments showed that B cell translocation gene 2 (BTG2) was a target of miR-21. The expression of BTG2 was significantly decreased both in myocardium and H9C2 cells treated with DOX. The present study has revealed that miR-21 protects mouse myocardium and H9C2 cells against DOX-induced cardiotoxicity probably by targeting BTG2. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
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Functional role of tlyC1 encoding a hemolysin-like protein from Bifidobacterium longum BBMN68 in bile tolerance
作者：
Liu, Ying;An, Haoran;Zhang, Jingsheng;Zhou, Hui;Ren, Fazheng;...
期刊：
FEMS Microbiology Letters ,2014年360(2):167-173 ISSN：0378-1097
通讯作者：
Hao, Yanling
作者机构：
[Zhang, Jingsheng; Liu, Ying; Hao, Yanling; An, Haoran; Ren, Fazheng] China Agr Univ, Coll Food Sci & Nutr Engn, Key Lab Funct Dairy, Beijing 100083, Peoples R China.;[Zhou, Hui] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Hao, Yanling] China Agr Univ, Coll Food Sci & Nutr Engn, 17 Qing Hua East Rd, Beijing 100083, Peoples R China.
通讯机构：
[Hao, Yanling] C;China Agr Univ, Coll Food Sci & Nutr Engn, 17 Qing Hua East Rd, Beijing 100083, Peoples R China.
关键词：
Bifidobacterium longum BBMN68;hemolysin-like protein;function analysis;bile salt tolerance
摘要：
Bifidobacteria are normal inhabitants of the human gut, and members of which are generally considered to be probiotic. Before exerting their beneficial properties, they must survive and persist in the physiological concentrations (0.05–2%) of bile in the gut. In this work, the functional role of tlyC1 encoding a hemolysin-like protein from Bifidobacterium longum BBMN68 in bile tolerance was tested. Analysis using the program TMHMM and homologous alignment indicated that TlyC1 is a nontransporter membrane protein and is conserved in many bifidobacteria. Heterologous expression of tlyC1 in Lactococcus lactisNZ9000 was shown to confer 45-fold higher tolerance to 0.15% ox-bile. Notably, the recombinant strains showed threefold higher survival when exposed to sublethal concentration of TCA and TDCA, while no significant change was observed when exposed to GCA and GDCA. Furthermore, real-time quantitative PCR demonstrated that the transcription of tlyC1 was up-regulated c. 2.5- and 2.7-fold in B. longumBBMN68 exposed to sublethal concentration of TCA and TDCA, while no significant change was observed with GCA and GDCA challenges. This study indicated that tlyC1 was specifically induced by tauroconjugates, which provided enhanced resistance to sodium taurocholate and sodium taurodeoxycholate.
This work represents the first study on the non-transporter membrane protein involving in a new likely bile protection system in the genus Bifidobacterium.
This work represents the first study on the non-transporter membrane protein involving in a new likely bile protection system in the genus Bifidobacterium.
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