摘要：
The MYB proteins belong to a large family of transcription factors in plant genomes and play significant roles in primary and secondary metabolism. Although several CsMYB genes have been identified in Camellia sinensis, few CsMYBs involved in L-theanine biosynthesis have been analyzed. In this study, we screened and identified 20 CsMYBs related to L-theanine biosynthesis. Transcriptomic analysis revealed that the expression profiles of the CsMYBs were positively or negatively related to dynamic changes in the L-theanine content. Validation of selected L-theanine biosynthetic and CsMYB genes was conducted by qRT-PCR. The results illustrated that most of the structural and CsMYB genes were downregulated with a decrease in the L-theanine levels. Protein-protein interaction networks of CsMYB5, CsMYB12 and CsMYB94 proteins demonstrated that they might form complexes with bHLH and WD 40 proteins. Multiple DNA-binding sites of the R2R3-MYB protein were observed in promoter regions of structural genes, indicating CsMYB family proteins might be involved in L-theanine metabolism via the attachment of AC elements. Moreover, CsMYB73 demonstrated binding specificity to the promoter region of CsGDH2 (CsGDH2-pro). These findings provide fundamental understanding of specific members of the CsMYBs related to the L-theanine biosynthesis pathway. (C) 2020 Published by Elsevier B.V.
摘要：
Background: The amount of fluoride accumulation in tea leaves was gradually increase as the matures of tea plants, and the excessive fluoride intake can threaten people's health. Based on years of field investigations, a low level of fluoride variety Xiangbo Lu (XBL) and a high level of fluoride variety Zhenong 139 (ZN139) were selected. Results: In this study, the root, 1st and the 5th leaf of the two-year-old tea trees were used for morphological, physiological and comparative transcriptomics analysis to understand the different features of "XBL" and "ZN139" under fluoride stress conditions. The color of the 1st and 5th leaves of XBL were yellower, the activity of peroxidase, catalase and antioxidant enzyme were lower than ZN139 under the high-fluoride stress. Transcriptomics analysis indicated that core genes involved in photosynthesis rates regulation showed no significantly exchanged expression, the co-downregulation of magnesium ions transportation, while the ROS scavenging, vegetative growth and self-compatibility between the two varieties were different. Crucial genes' expression were also identified by the real-time RT-PCR. Conclusion: The tea tree is one of the few plants that has a high-fluoride content, but the different varieties respond differently to fluoride stress. High-fluoride tea tree varieties, such as ZN139, have stronger ROS scavenging abilities through the use of both their non-enzymatic and enzymatic antioxidant systems which act by increasing the expression levels of inositol-1-monophosphatases and peroxidases, among others. ZN139 can also compensate for the decrease in photosynthetic rate that is associated with the ionic imbalance caused by the reduced consumption of light energy during long-periods of high fluoride stress. Reproductive development was protected in ZN139 by the up-regulated expression of S-locus glycoprotein, Mildew resistance locus o and Phospholipase D under fluoride stress, while the vegetative development of low-fluoride varieties such as XBL was retarded. More starch and cellulose were redistributed to glucose by increasing the expression levels of glycosyl transferases and hydrolases to provide more energy for processes involved in the response and tolerance towards fluoride stress.
摘要：
l-Theanine, a non-proteinaceous amino acid abundantly present in tea (Camellia sinensis), contributes to the umami flavor of tea and has beneficial effects on human health. While key l-theanine biosynthetic genes have been well documented, their transcriptional regulation remains poorly understood. In this study, we determined the l-theanine contents in tea leaves of two cultivars at three developmental stages and investigated the expression patterns of the l-theanine biosynthetic genes CsGS1 and CsGS2. Additionally, we identified an R2R3-MYB transcription factor, CsMYB73, belonging to subgroup 22 of the R2R3-MYB family. CsMYB73 expression negatively correlated with l-theanine accumulation during leaf maturation. We found that CsMYB73, as a nuclear protein, binds to the promoter regions of CsGS1 and CsGS2 via MYB recognition sequences and represses the transcription of CsGS1 and CsGS2 in tobacco leaves. Collectively, our results demonstrate that CsMYB73 is a transcriptional repressor involved in l-theanine biosynthesis in tea plants. Our findings might contribute to future tea plant breeding strategies.
摘要：
Tea catechins, the main bioactive polyphenols in green tea, are well known for their health promoting effects. Previous studies have shown that gallocatechin-3-gallate (GCG), epigallocatechin-3-gallate (EGCG) and epicatechin-3-gallate (ECG) exerted strong inhibitory effects on mushroom tyrosinase activity in vitro, whilst EGCG inhibited melanogenesis in vivo, yet the underlying mechanisms are not entirely clear. In this study, we (i) evaluated and compared the inhibitory effects of the main tea catechins (GCG, EGCG, and ECG) on melanogenesis in B16F10 melanoma cells, and (ii) explain the underlying mechanisms. The results showed that the tea catechins significantly suppressed tyrosinase activity and melanin synthesis in B16F10 cells, where the effects of ECG > EGCG > GCG. Interestingly, the inhibitory effects of the catechins were stronger than those of arbutin (AT), a well-known depigmenting agent. Moreover, GCG, EGCG, and ECG regulated the melanogenesis of B16F10 cells through the cAMP/CREB/MITF pathway. These results revealed catechins could be used as anti-melanogenic agents to protect cells from abnormal melanogenesis.
摘要：
Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.
摘要：
Dark tea is a unique fermented tea produced by solid-state fermentation of tea leaves (Camellia sinensis). It includes ripe Pu-erh tea, Fu brick tea, Liupao tea, and other teas. Microbial fermentation is considered to be the key factor controlling the quality of dark tea. It involves a series of reactions that modify the chemical constituents of tea leaves. These chemical conversions during microbial fermentation of dark tea are associated with a variety of functional core microorganisms. Further, Multi-omics approaches have been used to reveal the microbial impact on the conversion of the chemical components in dark tea. In the present review, we provide an overview of the most recent advances in the knowledge of the microbial bioconversion of the chemical components in dark tea, including the chemical composition of dark tea, microbial community composition and dynamics during the fermentation process, and the role of microorganisms in biotransformation of chemical constituents.
摘要：
Epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3 '' Me) in tea (Camellia sinensis (L.) O. Kuntze) is a major source of O-methylated catechin and renowned for a wide range of health effects. However, the transcriptional regulation mechanisms of EGCG3 '' Me biosynthesis remain unclear. In the present work, the basic Helix-Loop-Helix (bHLH) transcription factor, designated as CsbHLH62, belonging to GBOF group of bHLH families, was isolated and characterized from Camellia sinensis. CsbHLH62 contains an Open Reading Frame of 1662 bp and encodes a polypeptide of 553 amino acids. Subcellular location and transcriptional activity analysis showed it as a nucleus protein and possessed transcriptional inhibition activity. Furthermore, the expression of CsbHLH62 was decreased during EGCG3 '' Me accumulation. More importantly, E-box motifs (5'-CANNTG-3') were found in the promoters of CCoAOMT, CsLAR, and CsDFR, and further transient expression assays showed that CsbHLH62 repressed the transcription of CCoAOMT, CsLAR, and CsDFR. Collectively, these results suggest that CsbHLH62 acts as a transcriptional repressor that might be negatively affecting the accumulation of EGCG3 '' Me. These findings provide novel insights into the regulatory mechanism of EGCG3"Me biosynthesis, which might help to breed high EGCG3 '' Me-content tea plants.
摘要：
Gut microbiota play a key role in the regulation of obesity and associated metabolic disorders. To study the relationship between them, antibiotics have been widely used to generate pseudo-germ-free rodents as control models. However, it is not clear whether antibiotics impact an animal's metabolic phenotype. Therefore, the effect of antibiotics-induced gut microbial perturbations on metabolic phenotypes in high-fat diet (HFD) fed mice was investigated. The results showed that antibiotics perturbed gut microbial composition and structure. Community diversity and richness were reduced, and the phyla Firmicutes/Bacteroidetes (F/B) ratio was decreased by antibiotics. Visualization of Unifrac distance data using principal component analysis (PCA) and unweighted pair-group method with arithmetic mean (UPGAM) demonstrated that fecal samples of HFD-fed mice separated from those of chow diet (CD) fed mice. Fecal samples from antibiotics-treated and non-treated mice were clustered into two different microbial populations. Moreover, antibiotics suppressed HFD-induced metabolic features, including body weight gain (BWG), liver weight (LW), epididymal fat weight (EFW), and serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), fasting blood glucose (FBG), and insulin (INS) significantly (P < 0.05). Lachnospiraceae, Ruminiclostridium and Helicobacter, biomarkers of mouse gut microbiota before treatment by antibiotics, were positively correlated with obesity phenotypes significantly (P < 0.05) and were decreased by (92.95 +/- 5.09) %, (97.73 +/- 2.09) % and (99.48 +/- 0.21) % respectively after 30 days of treatment by antibiotics. However, Bacteroidia were enriched in HFD-fed antibiotics-treated mice and were negatively correlated with obesity phenotypes significantly (P < 0.05). We suggested that the antibiotics-induced depletion of Lachnospiraceae, Ruminiclostridium, and Helicobacter, and the decrease in F/B ratio in gut microbiota played a role in the prevention of HFD-induced obesity in mice.
作者机构：
[张向娜; 刘冬敏; 罗勇; 温贝贝] Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, 410128, China;Natl. Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, 410128, China;Hunan Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha, 410128, China;[王英姿; 黄建安; 王坤波; 李娟] Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha, 410128, China, Natl. Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha, 410128, China, Hunan Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha, 410128, China
通讯机构：
[Huang, J.] K;Key Laboratory of Ministry of Education for Tea Science, China
摘要：
<jats:p>Ascorbate peroxidase (APX) acts indispensably in synthesizing L-ascorbate (AsA) which is pivotal to plant stress tolerance by detoxifying reactive oxygen species (ROS). Enhanced activity of APX has been shown to be a key step for genetic engineering of improving plant tolerance. However it needs a deeper understanding on the maintenance of cellular ROS homeostasis in response to stress. In this study, we identified and characterized an<jats:italic>APX</jats:italic>(<jats:italic>CaAPX</jats:italic>) gene from<jats:italic>Camellia azalea</jats:italic>. Quantitative real-time PCR (qRT-PCR) analysis showed that<jats:italic>CaAPX</jats:italic>was expressed in all tissues and peaked in immature green fruits; the expression levels were significantly upregulated upon cold and hot stresses. Transgenic plants displayed marked enhancements of tolerance under both cold and heat treatments, and plant growth was correlated with<jats:italic>CaAPX</jats:italic>expression levels. Furthermore, we monitored the activities of several ROS-scavenging enzymes including<jats:italic>Cu/Zn-SOD</jats:italic>,<jats:italic>CAT</jats:italic>,<jats:italic>DHAR</jats:italic>, and<jats:italic>MDHAR</jats:italic>, and we showed that stress tolerance was synchronized with elevated activities of ROS-scavenging. Moreover, gene expression analysis of ROS-scavenging enzymes revealed a role of<jats:italic>CaAPX</jats:italic>to orchestrate ROS signaling in response to temperature stresses. Overall, this study presents a comprehensive characterization of cellular response related to<jats:italic>CaAPX</jats:italic>expression and provides insights to breed crops with high temperature tolerances.</jats:p>
