作者机构：
[Y.M.Ma; P.Wu; B.D.Gao] Hunan University Key Laboratory for Control and Utilization of Plant Diseases,Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Hunan Agricultural University,Furong District;[Y.M.Ma; P.Wu; B.D.Gao] Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences
会议名称：
第十届国际植物病理学大会暨中国植物病理学会2013年学术年会
会议时间：
2013-08-25
会议地点：
中国北京
摘要：
<正>Acidovorax citrullI(AC),the causal agent of watermelon fruit blotch,is a quarantine pest in China,which requires zero tolerance of viable bacterium in the seed disinfection treatments.This paper re
摘要：
ARC-111 has potent topoisomerase I-targeting activity and pronounced antitumor activity. To design ARC-111 analogues with improved efficiency, we performed analyses on the quantitative structure–activity relationship of 22 ARC-111 analogues assessed in P388 tumor cells. First, the support vector regression (SVR) models were constructed and optimized based on literature descriptors (the low-dimensional descriptor space) and the worst descriptor elimination multi-round (WDEM) method. The optimized SVR model had greater generalization ability than multiple linear regression (MLR) and stepwise linear regression (SLR) in the independence test, which indicated that our nonlinear WDEM method could remove redundant descriptors more effectively, and our optimized SVR was a more powerful modeling technique. Second, to identify more accessible and effective descriptors, our modeling descriptors with clear meanings were selected from a large number of descriptors calculated by the software PCLIENT. Through the high-dimensional descriptor selection nonlinear method and the WDEM method, seven independent variable combinations with tens of descriptors were selected out of 2,923 descriptors. The seven corresponding SVR models performed better in the independent test, compared to MLR and SLR. The evaluation measures supported the excellent predictive power of the new models. According to the interpretability analysis of the SVR model, the regression significance of the model and the importance of single indicator were evaluated based on F tests. Our study offers some useful theories for understanding the function mechanism and finds parameters for designing ARC-111 analogues with enhanced antitumor activity.
期刊：
Journal of Food, Agriculture and Environment,2013年11(3):420-422 ISSN：1459-0255
作者机构：
College of Biosafety Science and Technology, Hunan Agricultural University, Changsha 410128, China;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pest, Changsha 410128, China;College of Agronomy, Hunan Agricultural University, Changsha 410128, China
关键词：
Actinomycetes;Identification;MRSA
摘要：
A total of 230 Actinomycetes were obtained from the soil of the Three-river headwater national nature reserve in Qinghai province, China. A strain, SJY056, was isolated after primary screening using a plate confrontation culture method followed by a fermentation filtrate inhibitory assay. The fermentation filtrate significantly inhibited the growth of MRSA after diluted for 5 folds with an inhibition halo of 21-mm in diameter. Results of phenotypic, physiological and biochemical analyses, as well as sequencing analysis of 16S rDNA indicated that SJY056 was a strain of Streptomyces atroolivaceus. The organic phase obtained after liquid-liquid extraction of the metabolites of SJY056 using ethyl acetate inhibited the growth of MRSA with a inhibition halo of 23-mm in diameter, while the inorganic phase had no inhibitory effect on the growth of MRSA. These results indicated that the metabolites of SJY056 contained MRSA antagonist that had potential antibiotic activities.
摘要：
Amaranthus mangostanus is a green vegetable which is also used as sudorific, febrifuge, emollient, lactogogue and a specific treatment for colic. This study was aimed to determine antibacterial and antifungal activities of the methanol, dichloromethane, petroleum ether and ethyl acetate extracts from different parts of A. mangostanus against microbes that cause plant or human diseases and food contamination. At concentrations ranging from 40-100 mg/mL, ethyl acetate extracts from leaves and stems exhibited concentration-dependent inhibition against Pseudomonas solanacearum Smith, Acidovorax avenae subsp. A. Citrulli, Rhizoctonia solani, Colletotrichum capsici (syd.) Butl, Pseudomonas aeruginosa, Bacillus cereus and Escherichia coli, but not Magnaporthe grisea, Alternaria alternata Keissler, Phytophtora parasitica var. nicotianae Tucker and Fusarium graminearum Sehw., with MIC values of 519-713 and 463-697 mg/mL, respectively, whereas the ethyl acetate extract from roots only showed antibacterial activity against P. solanacearum Smith with an MIC of 815 mg/mL. Ethyl acetate extracts from leaves and stems also showed inhibitory effect on the fungus R. solani, with MICs of 686 and 734 mg/mL, respectively. Consequently the information on the antimicrobial functions of the extracts can shed light on the discovery of natural products for management of plant and human infectious diseases.
摘要：
The NCII design (North Carolina mating design II) has been widely applied in studies of combining ability and heterosis. The objective of our research was to estimate how different base populations, sample sizes, testcross numbers and heritability influence QTL analyses of combining ability and heterosis. A series of Monte Carlo simulation experiments with QTL mapping were then conducted for the base population performance, testcross population phenotypic values and the general combining ability (GCA), specific combining ability (SCA) and Hmp (midparental heterosis) datasets. The results indicated that: (i) increasing the number of testers did not necessarily enhance the QTL detection power for GCA, but it was significantly related to the QTL effect. (ii) The QTLs identified in the base population may be different from those from GCA dataset. Similar phenomena can be seen from QTL detected in SCA and Hmp datasets. (iii) The QTL detection power for GCA ranked in the order of DH(RIL) based > F2 based > BC based NCII design, when the heritability was low. The recombinant inbred lines (RILs) (or DHs) allows more recombination and offers higher mapping resolution than other populations. Further, their testcross progeny can be repeatedly generated and phenotyped. Thus, RIL based (or DH based) NCII design was highly recommend for combining ability QTL analysis. Our results expect to facilitate selecting elite parental lines with high combining ability and for geneticists to research the genetic basis of combining ability.
作者机构：
[J.Zhong; X.H.Lei; B.D.Gao] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests,Hunan Agricultural University
会议名称：
第十届国际植物病理学大会暨中国植物病理学会2013年学术年会
会议时间：
2013-08-25
会议地点：
中国北京
会议论文集名称：
Abstracts of ICPP 2013 10th International Congress of Plant Pathology
摘要：
<正>A novel virus contained two segments of dsRNA was detected from HNZJOOl strain of Collectotrichum acutatum,a phytopathogenic fungus causing anthracnose diseases in immature fruit of pepper.The complete nucleotide sequences were determined.Spherical viruslike particles in the size about 40 nm in diameter was observed from the mycelial tissue preparation.Sequence
摘要：
Bemisia tabaci, the whitefly vector of Tomato yellow leaf curl virus (TYLCV), seriously reduces tomato production and quality. Here, we report the first evidence that infection by TYLCV alters the host preferences of invasive B. tabaci B (Middle East-Minor Asia 1) and Q (Mediterranean genetic group), in which TYLCV-free B. tabaci Q preferred to settle on TYLCV-infected tomato plants over healthy ones. TYLCV-free B. tabaci B, however, preferred healthy tomato plants to TYLCV-infected plants. In contrast, TYLCV-infected B. tabaci, either B or Q, did not exhibit a preference between TYLCV-infected and TYLCV-free tomato plants. Based on gas chromatography-mass spectrometry (GCMS)analysis of plant terpene volatiles, significantly more β-myrcene, thymene, β-phellandrene, caryophyllene, (+)-4-carene, and α-humulene were released from the TYLCV-free tomato plants than from the TYLCV-infected ones. The results indicate TYLCV can alter the host preferences of its vector Bemisia tabaci B and Q.
摘要：
Cereal cyst nematodes are the most important plant-parasitic nematodes on cereal crops in wheat producing areas of the world. Heterodera filipjevi was first reported in China in 2010. In this study, species-specific sequence characterized amplified region polymerase chain reaction (SCAR-PCR) assays for detection and identification of H. filipjevi from infected wheat roots and soil were developed. The species-specific primers were designed according to the randomly amplified polymorphic DNA (RAPD) markers amplified with random primer OPK16. A 646-bp specific fragment of sequence was generated, which characterized amplified regions in H. filipjevi. The detection limitation of the PCR assay was as low as 0.125 mu l second-stage juvenile (J2) lysate, 3.9 x 10(-3) mu l adult female lysate, and 10(-3) mu l cyst lysate. The method was able to detect the various stages (J2, J3, J4, and female) of H. filipjevi, and a single of nematode in 0.5 g of soil. H. filipjevi was detected by the method in two of six field samples, and one of those samples contained a mixed population of H. filipjevi and H. avenae. This study is the first to provide a definitive diagnostic assay for H. filipjevi in wheat roots and soil.
作者：
Jin Xue;Jing Li;Hoang-Anh Ta;Heng-Mu Zhang;Jian Yang;...
期刊：
Genome Announcements,2013年1(3) ISSN：2169-8287
通讯作者：
Zhang, H.-M.
作者机构：
[Hoang-Anh Ta] Plant Protection Research Institute, Hanoi, Vietnam;[Jian Yang; Yuan Meng; Pei-Pei Li; Jian-Ping Chen] College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha, China;[Jing Li; Heng-Mu Zhang] State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, and Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;College of Agriculture and Biotechnology (CAB), Zhejiang University, Hangzhou, China;[Jin Xue] College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha, China<&wdkj&>State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, and Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
通讯机构：
[Zhang, H.-M.] S;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Lab of Plant Protection, Biotechnology, Ministry of Agriculture, Inst of Virology, Biotechnology, Zhejiang Provincial Key Lab of Plant Virology, Zhejiang Academy of Agricultural Sci, Hangzhou, China
摘要：
The nucleotide sequences of the ten genomic segments of a Vietnam isolate of southern rice blacked-dwarf virus were determined. This complete genomic sequence will help to further understand the viral etiology (origin of viral pathogen) and phylogenetic relationships among fijiviruses.
