作者： Xie, Chun-yan;Wang, Qinhua;Li, Guanya;Fan, Zhiyong;Wang, Hong;...

期刊： Journal of the Science of Food and Agriculture,2019年99(13):6108-6113 ISSN：0022-5142

通讯作者： Wu, Xin

作者机构： [Xie, Chun-yan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Li, Guanya; Wang, Qinhua; Wu, Xin] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Li, Guanya; Wang, Qinhua; Wu, Xin] Hunan Prov Engn Res Ctr Hlth Live Stock & Poultry, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha, Hunan, Peoples R China.;[Li, Guanya; Wang, Qinhua; Fan, Zhiyong] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha, Hunan, Peoples R China.;[Wang, Hong] Meiya Haian Pharmaceut Co Ltd, Haian, Peoples R China.

通讯机构： [Wu, Xin] C;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.

关键词： intestinal development;uridine;uridine monophosphate;weaned piglets

摘要： BACKGROUND: Nucleotides are key constituents of milk, where they are utilized in cell replication, although there are limited studies for weaned piglets. This study evaluated the effects of uridine monophosphate (UMP) with uridine (UR) feed supplementation on the intestinal development and nucleotide transport in weaned piglets. RESULTS: Supplementation with UMP significantly increased (P < 0.05) plasma glucose, and UR supplementation significantly reduced (0.05 < P < 0.10) the plasma total cholesterol (TC) of piglets when compared with that of the control group, although non-significant difference (P > 0.05) in growth performance was observed among three groups. Piglets fed supplementary UR exhibited greater (P < 0.05) crypt depth in the duodenum and ileum when compared with those in the supplementary UMP and control groups. Real-time quantitative polymerase chain reaction (RT-qPCR) results revealed that UR supplementation increased (P < 0.05) the relative mRNA levels of genes encoding the transmembrane proteins ZO-1 and occludin in the duodenum mucosa, and ZO-1 in the jejunum mucosa (P < 0.05). Similarly, UR supplementation increased (P < 0.05) expression of solute carriers SLC28A1 and SLC29A1 in the duodenum mucosa. Conversely, claudin-1 expression in the duodenum mucosa was inhibited (P < 0.05) by dietary supplementation with UMP or UR. CONCLUSION: Collectively, our data indicated that dietary supplementation with UMP or UR was conducive to stimulating intestinal development and promoting nucleotide transport in weaned piglets. © 2019 Society of Chemical Industry. © 2019 Society of Chemical Industry

语种： 英文

作者： Gu, Xue L.;Song, Ze H.*;Li, Hao;Wu, Sarah;Wu, Shu S.;...

期刊： Journal of the Science of Food and Agriculture,2019年99(13):5646-5653 ISSN：0022-5142

通讯作者： Song, Ze H.;Fan, Zhi Y.

作者机构： [He, Xi; Gu, Xue L.; Wu, Shu S.; Li, Hao; Ding, Ya N.; Song, Ze H.; Fan, Zhi Y.] Hunan Agr Univ, Inst Anim Nutr, Minist Educ, Engn Res Ctr Feed Safety & Efficient Utilizat, Changsha, Hunan, Peoples R China.;[Yin, Yu L.; He, Xi; Gu, Xue L.; Wu, Shu S.; Li, Hao; Ding, Ya N.; Song, Ze H.; Fan, Zhi Y.] Hunan Agr Univ, Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Wu, Sarah] Univ Idaho, Biol Engn, Moscow, ID 83843 USA.;[Yin, Yu L.] Chinese Acad Sci, Inst Subtrop Agr, Hunan Prov Engn Res Ctr Hlth Livestock, Key Lab Agroecol Proc Subtrop Reg, Beijing, Peoples R China.;[Song, ZH; Fan, Zhi Y.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Song, ZH; Fan, ZY] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： Bacillus spp.;blood metabolites;isomaltooligosaccharide;lactational performance;milk composition;sows

摘要： Background In this study, we evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp. in perinatal diets on lactational performance, blood metabolites, and milk composition of sows. Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group). Results The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups. In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05). Furthermore, the highest proportion of fat in colostrum (0.05 P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05). The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05). Conclusion IMO and Bacillus spp. supplementation during the perinatal period improved the sows' lactational performance by affecting their metabolism and milk quality. Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance. (c) 2019 Society of Chemical Industry

语种： 英文

作者： Wang, Hongquan;Ding, Chunhua;Wang, Jing'an;Zhao, Xin;Jin, Shengzhen;...

期刊： Fish & Shellfish Immunology,2019年86:794-804 ISSN：1050-4648

通讯作者： Li, Yaoguo;Xiao, Tiaoyi

作者机构： [Wang, Hongquan; Luo, Hong; Li, Dongfang; Xiao, Tiaoyi; Li, YG; Xiao, TY; Ding, Chunhua; Zhao, Xin; Li, Rui; Jin, Shengzhen; Liang, Jian; Wang, Jing'an; Li, Yaoguo] Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.;[Wang, Hongquan; Xiao, Tiaoyi; Li, Yaoguo] Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Hunan, Peoples R China.

通讯机构： [Li, YG; Xiao, TY] H;Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.

关键词： Antioxidant factor;Ctenopharyngodon idella;Serum immune factor;Viral infection;cDNA cloning

摘要： The grass carp reovirus (GCRV) has been shown to cause lethal infections in the grass carp Ctenopharyngodon idella (C. idella). In order to investigate the immune response to GCRV infection, the full-length cDNA sequences of coagulation factor VIII (CiFVIII) and plasminogen (CiPLG) from C. idella were cloned and their involvement in the immune response was studied. The immunity factor levels in C. idella with different GCRV resistances were also analyzed. The full-length 2478 bp cDNA of CiFVIII contained an open reading frame of 1965 bp and encoded a putative polypeptide of 654 amino acid residues. The full-length 2907 bp cDNA of CiPLG contained an open reading frame of 2133 bp and encoded a putative polypeptide of 710 amino acid residues. CiFVIII was closely clustered with that of Clupea harengus. CiPLG was first clustered with those of Cyprinus carpio and Danio rerio. CiFVIII transcripts were most abundant in the liver and least in the skin. The highest expression level of CiPLG was observed in liver and the lowest in muscle. Expression levels of CiFVIII in gill, head kidney and spleen, and expression levels of CiPLG in gill, intestine and liver all reached the maximum at 72h post GCRV infection. In spleen, expression levels of CiFVIII and CiPLG were significantly positively correlated. The activities of T-AOC, LSZ and IgM in Rmale symbol were significantly higher than those in Omale symbol. Likewise, T-AOC and LSZ activities in F1 were significantly higher than f1 individuals (P<0.01). These results indicated that CiFVIII and CiPLG may play important roles in the immune response to GCRV infection. In addition, antioxidant ability and serum immune factor activity may confer a different viral resistance to C. idella.

语种： 英文

作者： Wu, X-L*;Xu, J.;Li, H.;Ferretti, R.;He, J.;...

期刊： ANIMAL GENETICS,2019年50(4):367-371 ISSN：0268-9146

通讯作者： Wu, X-L

作者机构： [Ferretti, R.; Tait, R. G., Jr.; Michell, T.; Wu, X-L; Xu, J.; Bauck, S.; Li, H.; Simpson, B.] Neogen GeneSeek, Bioinformat & Biostat, Lincoln, NE 68504 USA.;[Wu, X-L; Li, H.] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA.;[Xu, J.; Kachman, S. D.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA.;[He, J.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Q.; Qiu, J.] Neogen GeneSeek, Qual Assurance, Lincoln, NE 68504 USA.

通讯机构： [Wu, X-L] N;Neogen GeneSeek, Bioinformat & Biostat, Lincoln, NE 68504 USA.

关键词： bovine SNP chips;genotyping errors;mendelian errors;quality assurance

摘要： SNP arrays are widely used in genetic research and agricultural genomics applications, and the quality of SNP genotyping data is of paramount importance. In the present study, SNP genotyping concordance and discordance were evaluated for commercial bovine SNP arrays based on two types of quality assurance (QA) samples provided by Neogen GeneSeek. The genotyping discordance rates (GDRs) between chips were on average between 0.06% and 0.37% based on the QA type I data and between 0.05% and 0.15% based on the QA type II data. The average genotyping error rate (GER) pertaining to single SNP chips, based on the QA type II data, varied between 0.02% and 0.08% per SNP and between 0.01% and 0.06% per sample. These results indicate that genotyping concordance rate was high (i.e. from 99.63% to 99.99%). Nevertheless, mitochondrial and Y chromosome SNPs had considerably elevated GDRs and GERs compared to the SNPs on the 29 autosomes and X chromosome. The majority of genotyping errors resulted from single allotyping errors, which also included the opposite instances for allele ‘dropout’ (i.e. from AB to AA or BB). Simultaneous allotyping errors on both alleles (e.g. mistaking AA for BB or vice versa) were relatively rare. Finally, a list of SNPs with a GER greater than 1% is provided. Interpretation of association effects of these SNPs, for example in genome-wide association studies, needs to be taken with caution. The genotyping concordance information needs to be considered in the optimal design of future bovine SNP arrays. © 2019 Stichting International Foundation for Animal Genetics

语种： 英文

作者： Wang, Jianhua;Cheng, Jia;Li, Yulong;Yan, Huiling;Wu, Ping;...

期刊： Journal of Fish Biology,2019年94(5):714-724 ISSN：0022-1112

通讯作者： Zhang, Jianshe

作者机构： [Wang, Jianhua; Li, Yulong; Zhang, Jianshe; Chen, Lin; Wu, Ping; Chu, Wuying; Cheng, Jia] Changsha Univ, Dept Biosci & Environm Engn, Changsha 410003, Hunan, Peoples R China.;[Wang, Jianhua] Guangxi Normal Univ, Coll Life Sci, Guilin, Peoples R China.;[Liu, Li; Zhu, Xin; Yan, Huiling] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, Jianshe] C;Changsha Univ, Dept Biosci & Environm Engn, Changsha 410003, Hunan, Peoples R China.

关键词： follistatin;molecular cloning;muscle growth;myogenic regulatory factor;satellite cell activation

摘要： A full-length complementary (c)DNA sequence encoding follistatin-related protein 3 (fsrp-3) was determined from skeletal muscle in Chinese mandarin fish Siniperca chuatsi, its molecular structure was characterised and its function suggested. The putative structure of S. chuatsi Fsrp-3 contains an N-terminal domain and two follistatin domains. Quantitative reverse-transcription (qRT)-PCR assays revealed that fsrp-3 messenger (m)RNA was differentially expressed among assayed tissues and was highly expressed in heart and intestine. fsrp-3 mRNA exhibited increasing expression from the larval to the juvenile stage (500 g). To investigate the potential function of S. chuatsi fsrp-3 in muscle growth, we constructed a Fsrp-3 prokaryotic expression system and injected the purified Fsrp-3 fusion protein into the dorsal muscle. Fsrp-3 administration significantly influenced cross-section area, satellite cell activation frequency and nuclear density of S. chuatsi muscle fibres. Following Fsrp-3 treatment, the expression of myogenic regulatory factors was up-regulated and decline in the expression of myostatin was observed. The study revealed that Fsrp-3 may affect muscle growth by regulating myogenic regulatory factor expression and antagonizing myostatin function to initiate satellite cell activation and differentiation in S. chuatsi.

语种： 英文

作者： Li, Rui;Hou, Gaifeng;Song, Zehe;Wu, Cong;Zhao, Jianfei;...

期刊： Animal Science Journal,2019年90(8):977-989 ISSN：1344-3941

通讯作者： He, Xi;Hou, De-xing

作者机构： [Song, Zehe; Wu, Cong; He, Xi; Xiang, Xuxiang; Hou, Gaifeng; Zhao, Jianfei; Li, Rui; Fan, Zhiyong; Hou, De-xing; Sun, Xingdong] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Song, Zehe; Wu, Cong; He, Xi; Xiang, Xuxiang; Hou, Gaifeng; Zhao, Jianfei; Li, Rui; Fan, Zhiyong; Sun, Xingdong] Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Hou, De-xing] Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima 8900065, Japan.

通讯机构： [He, Xi] H;[Hou, De-xing] K;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima 8900065, Japan.

关键词： digestive physiology;growth performance;nursery pigs;nutrients digestibility;protein sources

摘要： The study compared the effects of selected proteins replacing fish meal in low-protein diets on piglets' growth performance, intestinal digestive physiology, and nitrogen digestion and metabolism. Five reduced CP, amino acid (AA)-supplemented diets containing 4% of either S50, HP300, concentrated degossypolized cottonseed protein (CDCP), P50, or fish meal were assigned to six pens with 11 pigs for a 28-day study period. Compared with fish meal, dietary proteins did not affect growth performance, apparent total tract digestibility (ATTD) of nutrients, serum hormone levels and biochemical parameters, apparent ileal digestibility (AID) of CP and most AA, colonic short-chain fatty acid (SCFA) contents, duodenal and ileal morphology, digestive enzyme activity, and pH in small intestine of piglets. However, HP300, CDCP, and P50 decreased (p < 0.05) fecal N excretion per weight gain. AID of Ile in S50 and HP300 and Glu in P50 were improved (p < 0.05), and AID of Gly in other proteins was reduced (p < 0.05). S50 and P50 lowered (p < 0.05) the contents of colonic isobutyric and isovaleric. S50 and HP300 reduced (p < 0.05) jejunal villus height. CDCP increased (p < 0.05) the pepsin activity in stomach. S50, HP300, and CDCP decreased (p < 0.05) pH in the proximal colon. Overall, the selected proteins could completely replace fish meal in low-protein diet without impairing piglets' growth via maintaining intestinal digestive physiology, and nitrogen digestion and metabolism.

语种： 英文

作者： He, Shaoping;Li, Si;Arowolo, Muhammed Adebayo;Yu, Qifang;Chen, Fu;...

期刊： Animal Science Journal,2019年90(3):401-411 ISSN：1344-3941

通讯作者： He, Jianhua

作者机构： [Arowolo, Muhammed Adebayo; Chen, Fu; Yu, Qifang; He, Shaoping; Li, Si; He, Jianhua; Hu, Ruizhi] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

通讯机构： [He, Jianhua] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

关键词： heat stress;performance;resveratrol;serum parameter;yellow-feather broiler

摘要： This study investigated the effect of dietary resveratrol supplementation on growth performance, rectal temperature, and serum parameters of yellow-feather broilers under heat stress. A total of 480 yellow-feather broilers (28-day-old) were randomly allotted to five groups with six replicates. A thermoneutral group (TN) (24 ± 2°C) received a basal diet and another four heat-stressed groups (37 ± 2°C for 8 hr/day and 24 ± 2°C for the remaining time) were fed the basal diet or basal diet with 200, 350, and 500 mg/kg resveratrol for 14 consecutive days. The results revealed that resveratrol supplementation improved average daily gain (p = 0.001), and decreased (p < 0.05) rectal temperature from d 3 when compared with heat-stressed group without resveratrol. In addition, supplementation with resveratrol at 350 or 500 mg/kg lowered (p < 0.05) the contents of corticosterone, adrenocorticotropic hormone, cholesterol, triglycerides, uric acid, malonaldehyde, and activities of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase, increased (p < 0.05) the levels of triiodothyronine, the ratio of triiodothyronine to thyroxine, total protein, glutathione, and activities of alkaline phosphatase, total superoxide dismutase, catalase, and glutathione peroxidase, though with few fluctuation. In conclusion, supplementation with resveratrol can improve the growth performance by positively regulating serum metabolic parameters and alleviating tissue oxidant damage of broilers under heat stress. © 2019 Japanese Society of Animal Science

语种： 英文

作者： Du, Fan;Wang, Lin*;Yang, Zhaoguang;Liu, Peng;Li, Deliang*

期刊： Environmental Science and Pollution Research,2019年26(10):10148-10158 ISSN：0944-1344

通讯作者： Wang, Lin;Li, Deliang

作者机构： [Du, Fan; Liu, Peng; Wang, Lin; Yang, Zhaoguang] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Yang, Zhaoguang] Cent S Univ, Ctr Environm & Water Resources, Changsha 410083, Hunan, Peoples R China.;[Li, Deliang] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Wang, Lin] C;[Li, Deliang] H;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.

关键词： Arsenobetaine;Bioindicator;Biotransformation;Heatmap;Heavy metal;Ionomics

摘要： Freshwater aquatic environment close to cities and industrial areas is more sensitive than marine environment. The freshwater shellfish Semisulcospira cancellate was introduced as a bioindicator to monitor the heavy metal contamination in the river through ionomic profiles and arsenic speciation. The shellfish samples were collected near four cities along the Xiang River in China. The concentrations of elements including Mg, Al, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Sr, Cd, Sn, Sb, Ba, and Pb were determined using ICP-MS. Multivariate statistical analyses such as Pearson’s correlation analysis and principle component analysis (PCA) were employed to identify the possible sources of the elements in the shellfish samples. Three principle components were extracted from the ionomic matrix and were associated with natural existence, biological pathways, and mining and smelting activities, respectively. The ionomic profiles of the shellfish samples were evaluated through hierarchical cluster analysis (HCA) which was exhibited in the form of heatmap. The shellfish samples were categorized according to the sampling sites with different contamination levels. Six As species including arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB), and arsenocholine (AsC) were separated and quantified using HPLC-ICP-MS. The concentrations of As(III) and As(V) were linearly increased with total As concentration increasing. However, the proportion of AsB was decreased with total As while the AsB concentration was irrelevant to total As. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Yano, Satoshi;Wu, Shusong;Sakao, Kozue;Hou, De-Xing*

期刊： Bioscience, Biotechnology, and Biochemistry,2019年83(5):960-969 ISSN：0916-8451

通讯作者： Hou, De-Xing

作者机构： [Sakao, Kozue; Yano, Satoshi; Hou, De-Xing] Kagoshima Univ, United Grad Sch Agr Sci, Course Biol Sci & Technol, Kagoshima, Japan.;[Wu, Shusong] Hunan Agr Univ, Coll Anim Sci & Technol, Dept Anim Nutr & Feed Sci, Changsha, Hunan, Peoples R China.;[Sakao, Kozue; Hou, De-Xing] Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima, Japan.

通讯机构： [Hou, De-Xing] K;Kagoshima Univ, United Grad Sch Agr Sci, Course Biol Sci & Technol, Kagoshima, Japan.;Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima, Japan.

关键词： Wasabi;apoptosis;ERK1/2;ELK1/CHOP/DR5;colorectal cancer cells

摘要： 6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a major bioactive compound in Wasabi. Although 6-MSITC is reported to have cancer chemopreventive activities in rat model, the molecular mechanism is unclear. In this study, we investigated the anticancer mechanisms using two types of human colorectal cancer cells (HCT116 p53+/+ and p53−/−). 6-MSITC caused cell cycle arrest in G2/M phase and induced apoptosis in both types of cells in the same fashion. Signaling data revealed that the activation of ERK1/2, rather than p53, is recruited for 6-MSITC-induced apoptosis. 6-MSITC stimulated ERK1/2 phosphorylation, and then activated ERK1/2 signaling including ELK1 phosphorylation, and upregulation of C/EBP homologous protein (CHOP) and death receptor 5 (DR5). The MEK1/2 inhibitor U0126 blocked all of these molecular events induced by 6-MSITC, and enhanced the cell viability in both types of cells in the same manner. These results indicated that ERK1/2-mediated ELK1/CHOP/DR5 pathway is involved in 6-MSITC-induced apoptosis in colorectal cancer cells. © 2019 Japan Society for Bioscience, Biotechnology, and Agrochemistry

语种： 英文

作者： Wang, Rong;Wang, Min*;Zhang, Xiu Min;Wen, Jiang Nan;Ma, Zhi Yuan;...

期刊： Journal of Dairy Science,2019年102(3):2275-2282 ISSN：0022-0302

通讯作者： Deng, Jin Ping;Wang, Min

作者机构： [Wen, Jiang Nan; Long, Dong Lei; Deng, Jin Ping; Wang, Rong] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Wen, Jiang Nan; Tan, Zhi Liang; Long, Dong Lei; Wang, Rong; Zhang, Xiu Min; Ma, Zhi Yuan; Wang, Min] Chinese Acad Sci, Inst Subtrop Agr, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.;[Tan, Zhi Liang; Wang, Min] Hunan Co Innovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.;[Deng, Jin Ping] South China Agr Univ, Coll Anim Sci, Subtrop Inst Anim Nutr & Feed, Guangdong Prov Key Lab Anim Nutr Control, Guangzhou 510642, Guangdong, Peoples R China.

通讯机构： [Deng, Jin Ping; Wang, Min] H;[Wang, Min] C;[Deng, Jin Ping] S;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Sci, Inst Subtrop Agr, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.

关键词： dissolved hydrogen;dissolved methane;methanogenesis;rumen fermentation

摘要： Rumen cannulation is a widely employed technique in ruminant nutrition research. However, the gap between skin and rumen cannula can cause leakage of fermentation gases and influx of atmospheric air, which may adversely affect the anaerobic environment in the rumen. The present study was designed to investigate the effects of rumen cannulation on headspace gases, dissolved gases, fermentation end products, and methanogen community in the rumen of dairy cows. Eight Holstein cows were used in the experiment. Four cows were surgically fitted with rumen cannulas, whereas the other 4 intact cows were used as control. Rumen cannulation decreased gaseous hydrogen and methane concentrations, dissolved carbon dioxide concentration, and relative abundances of Methanosphaera, and increased the saturation factor of dissolved hydrogen and dissolved methane, dissolved methane concentration, volatile fatty acid concentration, 16S ribosomal RNA gene copies of methanogens, and Simpson index of methanogen community. In summary, rumen cannulation causes a reduction in headspace gaseous hydrogen and gaseous methane, which may not decrease dissolved gas concentrations due to an increase in saturation factors. Furthermore, rumen cannulation alters methanogen community with increased methanogen population and decreased relative abundances of Methanosphaera.

语种： 英文

作者： Liu, Xu;Peng, Canyang;Qu, Xiangyong*;Guo, Songchang*;Chen, Ji Fa;...

期刊： JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION,2019年103(1):182-190 ISSN：0931-2439

通讯作者： Qu, Xiangyong;Guo, Songchang

作者机构： [Liu, Xu; Guo, Songchang; He, Changqing; Chen, Ji Fa; Qu, Xiangyong; Guo, SC; Peng, Canyang] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Liu, Xu; Guo, Songchang; He, Changqing; Chen, Ji Fa; Qu, Xiangyong; Peng, Canyang] Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Zhou, Xuebin] Shanghai Naseco Prod Co, Shanghai, Peoples R China.;[Zhu, Shiwei] Hunan Songyun Commercial Fowl Co, Huaihua, Peoples R China.

通讯机构： [Qu, XY; Guo, SC] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

关键词： antioxidant capacity;Bacillus subtilis;egg quality;immune response;laying breeders;performance

摘要： To investigate the supplemental effects of Bacillus subtilis C-3102 on the production, hatching performance, egg quality, serum antioxidant capacity and immune response of laying breeders, a total of 480 Xuefeng black-bone (25-week-old) hens were randomly assigned into four treatment groups: Hens fed the basal diets with 0 (CON), 3.0 × 10 5 (BS-1), 6.0 × 10 5 cfu/g (BS-2) and 9.0 × 10 5 (BS-3) cfu/g of B. subtilis C-3102. As the B. subtilis C-3102 level increased, egg weight (linear, p < 0.01; quadratic, p = 0.003), fertility (linear, p = 0.021; quadratic, p = 0.059), hatchability (linear, p = 0.038; quadratic, p = 0.119) and yolk colour (linear, p = 0.006; quadratic, p = 0.021) increased in a linear or quadratic manner. Yolk index increased quadratically (linear, p = 0.054; quadratic, p = 0.017), and eggshell thickness (linear, p = 0.036; quadratic, p = 0.128), the activity of GSH-Px (linear, p = 0.024; quadratic, p = 0.078), the concentration of IgM (linear, p = 0.016; quadratic, p = 0.056) and the level of AIV-Ab (linear, p = 0.034; quadratic, p = 0.103) in the serum increased linearly as dietary supplementation of B. subtilis C-3102 increased. The results showed that dietary treatments did not affect egg production, feed conversion ratio, egg mass, hatchability of fertile eggs, eggshell-breaking strength, egg-shape index, yolk percentage, Haugh unit, T-SOD, T-AOC, MDA, IgA and IgG concentrations and the level of NDV-Ab in the serum. In conclusion, dietary supplementation of 9.0 × 10 5 cfu/g B. subtilis C-3102 in laying breeders diets may be a feasible means of effectively increasing egg weight, fertility and hatchability, and improving egg quality such as eggshell thickness, yolk index and yolk colour. Besides, B. subtilis C-3102 can enhance the activity of GSH-Px, the concentration of IgM and the level of AIV-Ab in the serum. © 2018 Blackwell Verlag GmbH

语种： 英文

作者： Zhu, Dan;Ma, Yong;Ding, Sujuan;Jiang, Hongmei;Fang, Jun*

期刊： BioMed Research International,2018年2018:2607679 ISSN：2314-6133

通讯作者： Fang, Jun

作者机构： [Zhu, Dan; Ding, Sujuan; Ma, Yong; Jiang, Hongmei; Fang, Jun] Hunan Agr Univ, Coll Anim Sci & Technol, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Fang, Jun] H;Hunan Agr Univ, Coll Anim Sci & Technol, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

摘要： This study investigated the antioxidant capacity and intestinal bacteria community in a mouse model of DSS-induced colitis. Twenty mice were randomly assigned to two treatments: Mice with colitis induced by 5% DSS (DSS group) and mice with colitis induced by 5% DSS that also received melatonin treatment (MEL group). The DSS group showed significantly less antioxidant capability than the MEL group, but the two groups did not differ significantly in terms of diversity index (Shannon and Simpson), bacterial culture abundance (Chao1 and ACE), and coverage (Good's coverage estimator). Bacteroidetes were the most abundant phylum in the DSS group (58.93%), followed by Firmicutes with 31.46% and Proteobacteria with 7.97%. In contrast, Firmicutes were the most abundant in the MEL group (49.48%), followed by Bacteroidetes with 41.63% and Proteobacteria with 7.50%. The results support the use of melatonin for prevention of intestinal bowel disease due to its modulatory effect on antioxidant capability and microbiota in mice with colitis. Melatonin was demonstrated to improve the oxidative stress resistance of mice with colitis and regulate the intestinal microbial flora, thus improving intestinal health. © 2018 Dan Zhu et al.

语种： 英文

作者： Gao, Lu-min;Xie, Chun-yan*;Zhang, Tian-yong;Wu, Xin*;Yin, Yu-long

期刊： Biochemical and Biophysical Research Communications,2018年505(2):624-630 ISSN：0006-291X

通讯作者： Xie, Chun-yan;Wu, Xin

作者机构： [Gao, Lu-min; Wu, Xin; Xie, Chun-yan; Yin, Yu-long] Hunan Agr Univ, Coll Anim Sci & Technol, Coll Biosci & Biotechnol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.;[Gao, Lu-min; Wu, Xin; Yin, Yu-long] Chinese Acad Sci, Minist Agr,Sci Observing & Expt Stn Anim Nutr & F, Key Lab Agroecol Proc Subtrop Reg,Hunan Prov Engn, Inst Subtrop Agr,Natl Engn Lab Pollut Control & W, Changsha 410125, Hunan, Peoples R China.;[Zhang, Tian-yong] Henan Guangan Biol Technol Co Ltd, Zhengzhou 450001, Henan, Peoples R China.

通讯机构： [Xie, CY; Wu, X] H;Hunan Agr Univ, Coll Anim Sci & Technol, Coll Biosci & Biotechnol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.

关键词： *Calcium;*Circadian clock;*Feeding time;*Lipid metabolism;*Sows;*Transport

摘要： To investigate effects of Ca level varying with feeding time daily in sows during late pregnancy on placental lipid metabolism and transport in pigs, sixty pregnant sows were assigned to 3 groups: the CON group was fed low-Ca diet with 11.25 g CaCO3 at 0600 h and 1500 h, H-L group was fed low-Ca diet with 22.5 g CaCO3 at 0600 h and low-Ca diet at 1500 h, and L-H group was fed low-Ca diet at 0600 h and low-Ca diet with 22.5 g CaCO3 at 1500 h, respectively. Serum from sows and umbilical cord and placenta were collected during delivery. Results showed that, compared with the CON group, H-L feeding significantly increased maternal serum total triglyceride (TG) and umbilical serum high-density lipoprotein (HDL) (P < 0.05). The results showed that long chain fatty acid (FA) contents in placenta were significantly increased in H-L and L-H groups (P < 0.05). Experiments on genes involved in glycolipid metabolism showed that H-L or L-H feeding inhibited mRNA expression of GLUT3, GLUT4, FAS, FABP1, FABPpm, FAT/CD36, while activated the mRNA expression of FASD1, FASD2 and SCD in placenta (P < 0.05). In addition, experiments on genes involved in biological clock showed that L-H feeding sequence activated the mRNA expression of per1 and clock, while H-L and L-H feeding sequence inhibited mRNA expression of per2 in placenta (P < 0.05). It is concluded that maternal supplementation with Ca varying with feeding time daily during late pregnancy affects placental lipid metabolism and transport in pigs by regulating the mRNA expression related to lipid metabolism and the circadian clock. © 2018 Elsevier Inc.

语种： 英文

作者： Chen, Jiashun;Kang, Baoju;Zhao, Yurong;Yao, Kang;Fu, Chenxing*

期刊： JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION,2018年102(6):1666-1674 ISSN：0931-2439

通讯作者： Fu, Chenxing

作者机构： [Chen, Jiashun; Yao, Kang; Fu, Chenxing; Kang, Baoju; Zhao, Yurong] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Chen, Jiashun; Yao, Kang] Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock,Inst Subtr, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Key Lab Agroecol Proc Subtrop Reg,Minist Agr, Changsha, Hunan, Peoples R China.;[Chen, Jiashun; Fu, Chenxing] Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha, Hunan, Peoples R China.;[Chen, Jiashun; Fu, Chenxing] Hunan Collaborat Innovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.

通讯机构： [Fu, Chenxing] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： growth performance;intestinal morphology;microflora;sanguinarine;weaned piglets

摘要： This study was conducted to investigate the effects of dietary supplementation with Sangrovit® (SAG; minimum of 1.5% sanguinarine, a quaternary benzo[c]phenanthridine alkaloid extracted from Macleaya cordata) on growth performance, intestinal morphology, intestinal microflora and its metabolites of early-weaned piglets. A total of 20 healthy weaned piglets (Duroc× [Large White×Landrace]), weaned at 21 days of age with an average body weight (BW) of 6.52 ± 0.23 kg, were randomly assigned to receive either a corn-soybean meal basal diet (CTR) or a basal diet supplemented with 50 mg/kg SAG (SAG). During the 21-days trial, we collected and analysed intestinal tissues and the luminal digesta for their morphology and populations of gut microbiota, as well as for measuring the concentrations of short-chain fatty acids (SCFAs) and ammonia. Compared with the CTR group, supplementation with SAG improved average daily gains (p = 0.011) and average daily feed intake (p = 0.037). Piglets fed the SAG diet had an average lower value for crypt depth of the jejunum (p = 0.011) and greater values for villus height in the ileum (p = 0.015) and ratios of villus height to crypt depth in the jejunum (p < 0.01) and in the ileum (p = 0.027) than did animals receiving the CTR diet. The addition of SAG increased the amounts of Lactobacillus in the ileum (p = 0.033) and caecum (p < 0.01), and tended to increase the amounts of Bifidobacterium (p = 0.058) in the caecum, while decreasing the amounts of Escherichia coli (p = 0.046) and Salmonella spp. (p = 0.035) in the ileum, as well as Salmonella spp. (p = 0.029) in the caecum. Dietary supplementation with SAG enhanced (p < 0.05) the concentrations of acetate, propionate, butyrate and total SCFAs, and also tended to increase the level of valerate (p = 0.055 and p = 0.052) in the ileal and caecal contents when compared with the CTR group. Concentrations of ammonia also declined in the caecal (p = 0.037) and ileal (p = 0.046) digesta in response to SAG. These results indicate that feeding early-weaned piglets a SAG-supplemented diet can potentially improve their growth performance and intestinal morphology, and can modify the intestinal luminal environment in a beneficial manner. © 2018 Blackwell Verlag GmbH

语种： 英文

作者： Chen, Jingshu;Zhon, Lei;Wu, Jing;Ke, Sui;Morpurgo, Benjamin;...

期刊： JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2018年2018(136) ISSN：1940-087X

通讯作者： Tian, Yanan

作者机构： [Ke, Sui; Guo, Shaodong; Chen, Jingshu; Tian, Yanan; Sun, Yuxiang] Texas A&M Univ, College Stn, TX 77843 USA.;[Wu, Jing; Zhon, Lei; Tian, Yanan] Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha, Hunan, Peoples R China.;[Morpurgo, Benjamin; Golovko, Andrei] Texas A&M Inst Genom Med, College Stn, TX 77845 USA.;[Ouyang, Nengtai] Sun Yat Sen Mem Hosp, Guangzhou, Guangdong, Peoples R China.

通讯机构： [Tian, Yanan] H;[Tian, Yanan] T;Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha, Hunan, Peoples R China.;Texas A&M Univ, College Stn, TX 77843 USA.

关键词： Diabetogenic chemicals;Immunology and Infection;Issue 136;Pancreatic islets;ROS;Reactive oxygen species;T2DM;Xenobiotics

摘要： Exposure to certain environmental chemicals in human and animals has been found to cause cellular damage of the pancreatic β cells which will lead to the development of type 2 diabetes mellitus (T2DM). Although the mechanisms for the chemical-induced β cell damage were unclear and likely to be complex, one recurring finding is that these chemicals induce oxidative stress leading to the generation of excessive reactive oxygen species (ROS) which induce damage to the β cell. To identify potential diabetogenic environmental chemicals, we isolated pancreatic islet cells from C57BL/6 mice and cultured islet cells in 96-well cell culture plates; then, the islet cells were dosed with chemicals and the ROS generation was detected by 2',7'-dichlorofluorescein (DCFH-DA) fluorescent dye. Using this method, we found that bisphenol A (BPA), Benzo[a]pyrene (BaP), and polychlorinated biphenyls (PCBs), could induce high levels of ROS, suggesting that they may potentially induce damage in islet cells. This method should be useful for screening diabetogenic xenobiotics. In addition, the cultured islet cells may also be adapted for in vitro analysis of chemical-induced toxicity in pancreatic cells. © 2018, Journal of Visualized Experiments. All rights reserved.

语种： 英文

作者： Luo, Wei;Deng, Zhen-han;Li, Rui;Cheng, Guo;Kotian, Ronak Naveenchandra;...

期刊： BIOSCIENCE REPORTS,2018年38(1):BSR20171554. ISSN：0144-8463

通讯作者： Li, Yu-sheng;Li, Wen-ping

作者机构： [Li, Yu-sheng; Luo, Wei; Deng, Zhen-han] Cent S Univ, Dept Orthopaed, Xiangya Hosp, Changsha, Hunan, Peoples R China.;[Li, Rui; Li, Wen-ping; Cheng, Guo] Hunan Agr Univ, Dept Anim Sci, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Kotian, Ronak Naveenchandra] Victoria Hosp, Bangalore Med Coll & Res Inst, Dept Orthopaed Surg, Bangalore, Karnataka, India.

通讯机构： [Li, Yu-sheng] C;[Li, Wen-ping] H;Cent S Univ, Dept Orthopaed, Xiangya Hosp, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Dept Anim Sci, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

关键词： *5-HT;*NE;*analgesic activity;*earthworm extraction

摘要： Pain represents a major clinical problem and one which has exercised generations of healthcare professionals. Earthworms are used as a traditional Chinese medicine, and have been applied pharmacologically and clinically since a long time in China. However, the analgesic effects of earthworm extract (EE) are seldom studied. Hence, we evaluated the analgesic effects of EE inmice. The obtained data showed that EE increased pain threshold and exhibited peripheral but not central analgesic effects in mice; evidenced by increased inhibition ratio in acetic acid writhing test and formalin test, whereas only slight increase in inhibition ratio in hot plate test and tail immersion test. In addition, EE decreased serum norepinephrine (NE), 5-hydroxytryptamine (5-HT), and nitric oxide (NO) synthase (NOS) concentration, similar to other analgesic drugs like morphine and aspirin. In a nutshell, the obtained data have demonstrated that EE has peripheral analgesic properties and could be used as a promising analgesic drug.-c 2018 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).

语种： 英文

作者： Zhou, Xihong;Zhang, Yumei;Wu, Xin*;Wan, Dan;Yin, Yulong*

期刊： Cellular Physiology and Biochemistry,2018年48(3):993-1002 ISSN：1015-8987

通讯作者： Wu, Xin;Yin, Yulong

作者机构： [Zhou, Xihong; Wu, Xin; Wan, Dan; Yin, Yulong; Zhang, Yumei] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha, Hunan, Peoples R China.;[Zhou, Xihong; Wu, Xin; Wan, Dan; Yin, Yulong; Zhang, Yumei] Minist Agr, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Changsha, Hunan, Peoples R China.;[Wu, Xin; Yin, Yulong; Zhang, Yumei] Hunan Agr Univ, Coll Anim Sci & Technol, CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Zhou, Xihong] China Agr Univ, State Key Lab Anim Nutr, Beijing, Peoples R China.;[Wu, Xin; Yin, YL] Chinese Acad Sci, Inst Subtrop Agr, CAS 644 Yuanda 2nd Rd, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Wu, X; Yin, YL] C;Chinese Acad Sci, Inst Subtrop Agr, CAS 644 Yuanda 2nd Rd, Changsha 410125, Hunan, Peoples R China.

关键词： Inflammation;Intestine integrity;Oxidative stress;Serine;Weaning

摘要： Background/Aims: Early weaning often causes gut dysfunction. Since serine alleviates oxidative stress and inflammatory response which are accompany with early weaning, we conducted the study to explore whether serine improves intestinal function in early-weaned piglets. Methods: Twenty-eight weaned piglets (aged 21 d) were fed either a basal diet or a basal diet plus 0.2% serine. We determined the effects of dietary serine supplementation on intestinal morphology by hematoxylin and eosin staining, expression of tight junction proteins (TJPs) by immunoblotting and immunofluorescence, expression of inflammatory cytokines and apoptosis markers by RT-qPCR and the level of antioxidant enzymes with ELISA kits in early-weaned piglets. Results: Serine supplementation increased daily body weight gain while decreasing diarrhea incidence. Both the jejunum and ileum of serine-supplemented piglets showed regularly arranged villi and microvilli. Moreover, dietary serine increased TJP expression, and alleviated apoptosis, inflammation, and oxidative stress in the intestine of early-weaned piglets. Conclusion: Our findings suggest that serine has the potential for use as a feed additive to prevent gut dysfunction caused by weaning. © 2018 The Author(s). Published by S. Karger AG, Basel.

语种： 英文

作者： Ye, Hua*;Xiao, Shijun;Wang, Xiaoqing;Wang, Zhiyong;Zhang, Zhengshi;...

期刊： Marine Biotechnology,2018年20(2):246-256 ISSN：1436-2228

通讯作者： Ye, Hua;Luo, Hui

作者机构： [Ye, H; Luo, Hui; Zheng, Shuming; Zhang, Zhengshi; Hu, Bingjie; Zhu, Chengke; Ye, Hua] Southwest Univ, Coll Anim Sci, Chongqing 402460, Peoples R China.;[Ye, H; Luo, Hui; Zheng, Shuming; Zhu, Chengke; Ye, Hua] Minist Educ, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing, Chongqing 400175, Peoples R China.;[Luo, Hui; Wang, Xiaoqing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Wang, Xiaoqing] Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Peoples R China.;[Xiao, Shijun; Lv, Changhuan; Wang, Zhiyong] Jimei Univ, Coll Fisheries, Key Lab Hlth Mariculture East China Sea Agr, Minist Agr, Xiamen 361021, Peoples R China.

通讯机构： [Ye, H; Luo, H] S;[Ye, H; Luo, H] M;[Luo, Hui] H;Southwest Univ, Coll Anim Sci, Chongqing 402460, Peoples R China.;Minist Educ, Key Lab Freshwater Fish Reprod & Dev, Key Lab Aquat Sci Chongqing, Chongqing 400175, Peoples R China.

关键词： Aeromonas hydrophila;Differentially expressed genes;Immune-related gene;Schizothorax prenanti;Transcriptome

摘要： Schizothorax prenanti (S. prenanti) is an indigenous fish species and is popularly cultured in southwestern China. In recent years, intensive farming of S. prenanti and water quality deterioration has increased the susceptibility of this fish to various pathogens, including Aeromonas hydrophila (A. hydrophila), which has caused severe damage to S. prenanti production. However, the understanding of molecular immune response of S. prenanti to A. hydrophila infection is still lacking. In order to better comprehend the S. prenanti time series immune response process against A. hydrophila, we conducted the first transcriptomic comparison in S. prenanti spleen at 4, 24, and 48 h after the infection challenge of A. hydrophila against their control counterparts. In total, 628 million clean reads were obtained from 18 libraries and assembled into 262,745 transcripts. After eliminating sequence redundancy, 69,373 unigenes with an average length of 1476 bp were obtained. Comparative analysis revealed 1890 unigenes with significantly differential expression, including 172, 455, 589 upregulated and 27, 676, 551 unigenes downregulated genes for 4, 24, and 48 h post-infection, respectively. Differentially expressed genes (DEGs) were validated using qPCR for 15 randomly selected genes. Enrichment and pathway analysis of DEGs was carried out to understand the functions of the immune-related genes. Our results revealed that many important functional genes relating to complement and coagulation cascades, chemokine signaling pathway, toll-like receptor signaling pathway, NOD-like receptor signaling pathway and leukocyte transendothelial migration were regulated during the infection of A. hydrophila, and the expression of those genes reflected the transcriptome profiles during the challenging stages.

语种： 英文

作者： Jin, Shengzhen;Zhao, Xin;Wang, Hongquan;Su, Jianming;Wang, Jing'an;...

期刊： Fish & Shellfish Immunology,2018年83(1):292-307 ISSN：1050-4648

通讯作者： Li, Yaoguo;Xiao, Tiaoyi

作者机构： [Wang, Hongquan; Xiao, Tiaoyi; Li, Yaoguo; Xiao, TY; Zhao, Xin; Ding, Chunhua; Jin, Shengzhen; Wang, Jing'an; Su, Jianming] Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.;[Wang, Hongquan; Xiao, Tiaoyi; Li, Yaoguo] Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Hunan, Peoples R China.;[Su, Jianming] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Li, YG; Xiao, TY] H;Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.

关键词： Squaliobarbus curriculus;cDNA cloning;Structure comparison;Expression change;Correlation analysis

摘要： The barbel chub (Squaliobarbus curriculus) is a kind of small size commercial fish species that is widely spread in Asia and has shown significant resistance to disease. In this study, the full-length cDNA sequences of Toll-like receptor (TLR) 7 and 8 from S. curriculus, designated as ScTLR7 and ScTLR8, were cloned, and their differences in the structure and the responses to the grass carp (GCRV) infection and lipopolysaccharide stimulation were investigated. The full-length 3715 base pair (bp) cDNA of ScTLR7 contained a complete open reading frame of 3162 bp and encoded a putative polypeptide of 1053 amino acid residues. The full-length 4624 base pair (bp) cDNA of ScTLR8 contained a complete open reading frame of 3072 bp and encoded a putative polypeptide of 1023 amino acid residues. ScTLR7 and ScTLR8 consisted of N-terminal signal peptide, leucine-rich repeats (LRRs), and Toll/IL-1 Receptors domain. LRR motifs of ScTLR7 and ScTLR8 bend into horseshoe-like solenoid structure, while the number of LRRs between the two genes is different. Phylogenetic analysis showed that both the ScTLR7 and ScTLR8 were closely clustered with Ctenopharyngodon idellus and Megalobrama amblycephala. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScTLR7 in S. curriculus were most abundant in the brain followed by the spleen and heart, and the lowest in the intestine. The highest expression level of ScTLR8 was observed in spleen and the lowest in the liver. After LPS stimulation, the relative expression levels of both ScTLR7 and ScTLR8 exhibited an overall trend of up-regulation. The expression levels of type I-IFN showed an overall trend of down-regulation at time points of 12, 24, 72 and 168 h compared to that of 6 h after LPS stimulation. Compared to 6 h post GCRV infection, the transcription level of ScTLR7 was up-regulated from 12 to 168 h, and transcription levels of ScTLR8, MyD88, and type I-IFN were firstly up-regulated from 12 to 72 h, and then down-regulated from 72 to 168 h. Correlation analysis showed that expression level of ScTLR7 in the spleen was significantly positively correlated with that of MyD88 (Pearson correlation coefficient: 0.909, P: 0.033), and a significantly positive correlation was also observed between expression levels of MyD88 and type I IFN (Pearson correlation coefficient: 0.962, P: 0.009), after GCRV stimulation. These results indicate that ScTLR7 and ScTLR8 may play important roles in the responses to the grass carp (GCRV) infection and lipopolysaccharide stimulation and trigger different downstream immune signal pathways. © 2018 Elsevier Ltd

语种： 英文

作者： Liu, Lei;Yao, Li;Peng, Tao;Wen, Lixin;Cai, Wenjie;...

期刊： Journal of Dairy Research,2018年85(2):163-166 ISSN：0022-0299

通讯作者： Jia, Xinglin;He, Jianhua

作者机构： [Jia, Xinglin; Peng, Tao; Wen, Lixin; Yao, Li; Liu, Lei] Hunan Agr Univ, Coll Vet Med, Hunan Collaborat Innovat Ctr Safety Prod Livestoc, Changsha 410128, Hunan, Peoples R China.;[Liu, Lei; He, Jianhua] Hunan Agr Univ, Coll Anim Sci, Changsha 410128, Hunan, Peoples R China.;[Cai, Wenjie] Hunan Inst Anim & Vet Sci, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Jia, Xinglin; He, Jianhua] H;Hunan Agr Univ, Coll Vet Med, Hunan Collaborat Innovat Ctr Safety Prod Livestoc, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Anim Sci, Changsha 410128, Hunan, Peoples R China.

关键词： Dairy;liver;oxidative stress;perinatal period;metabolic diseases

摘要： The experiments reported in this research communication aimed to plot the expression pattern of Sirt3, a master regulator of energy metabolism and antioxidation defence, in the liver of dairy goats during perinatal period. Ten healthy dairy goats in late pregnancy were chosen, and needle biopsy was applied to collect liver samples at 1-week intervals. Protein levels of hepatic Sirt3 were analysed by western-blotting. Serum enzyme activities of manganese superoxide dismutase (Mn-SOD) and non-esterified fatty acids (NEFA) levels were measured, and their correlation with Sirt3 mRNA levels was also estimated. Compared with >3-week before parturition (BP), Sirt3 proteins were significantly reduced at 1-week after parturition (AP) and 2-week AP (P < 0·05), but increased on the day of parturition (P < 0·01). Correlation analysis revealed a positive association between hepatic Sirt3 mRNA levels and serum enzyme activity of Mn-SOD (r = 0·46), but a negative association between that and serum NEFA levels (r = -0·41). These data indicate that the decreased hepatic expression of Sirt3 might be one of the reasons that dairy goats undergo oxidative stress after parturition. Copyright © Hannah Dairy Research Foundation 2018.

语种： 英文