作者： Li, Deliang;Pi, Jie;Zhang, Ting*;Tan, Xiang;Fraser, Dylan J.

期刊： Environmental Science and Pollution Research,2018年25(21):21127-21137 ISSN：0944-1344

通讯作者： Zhang, Ting

作者机构： [Pi, Jie; Li, Deliang] Hunan Agr Univ, Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Zhang, Ting] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Tan, Xiang] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Hubei, Peoples R China.;[Fraser, Dylan J.] Concordia Univ, Dept Biol, Montreal, PQ, Canada.

通讯机构： [Zhang, Ting] C;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.

关键词： Bellamya aeruginosa;Bioaccumulation;Risk assessment;Sediment;Trace metals

摘要： Effective remediation of heavy metal pollution in aquatic systems is desired in many regions, but it requires integrative assessments of sediments, water, and biota that can serve as robust biomonitors. We assessed the effects of a 5-year metal contamination remediation along the Xiangjiang River, China, by comparing concentrations of trace metals in water and surface sediments between 2010–2011 and 2016. We also explored the trace metal biomonitoring potential of a freshwater gastropod (Bellamya aeruginosa). Metal concentrations in water (means and ranges) dropped over time to within permissible limits of drinking water guidelines set by China, USEPA, and WHO in 2016. Although sediment means and ranges of Cd, Pb, Zn, and Mn also diminished with remediation, those for Cr and Cu slightly increased, and all six metals retained concentrations higher than standards set by China. All metals in sediments could also be associated with anthropogenic inputs using a hierarchical clustering analysis, and they generate high potential ecological risks based on several indices, especially for Cd and As. The bio-sediment accumulation factors of all measured trace metals in gastropod soft tissues and shells were lower than 1.0, except for Ca. Trace metal contents in gastropods were positively correlated with those in water and surface sediments for As (soft tissues) and Cr (shells). Collectively, our results do not yet highlight strong beneficial effects of 5-year remediation and clearly illustrate the heavy metal pollution remaining in Xiangjiang River sediment. Additional physical, chemical, and biological measurements should be implemented to improve sediment quality. We further conclude that gastropod soft tissues and shells can be suitable biomonitors of spatial differences in some heavy metals found within river sediments (e.g., As, Cr). © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Yin, Jie;Ren, Wenkai;Huang, Xingguo;Li, Tiejun*;Yin, Yulong*

期刊： BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER,2018年1869(2):256-262 ISSN：0304-419X

通讯作者： Li, Tiejun;Yin, Yulong

作者机构： [Yin, Jie; Li, Tiejun; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.;[Yin, Jie; Li, Tiejun; Yin, Yulong] Minist Agr, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Changsha, Hunan, Peoples R China.;[Yin, Jie; Li, Tiejun; Yin, Yulong] Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Changsha, Hunan, Peoples R China.;[Yin, Jie] Univ Chinese Acad Sci, Beijing, Peoples R China.;[Ren, Wenkai; Yin, Yulong] South China Agr Univ, Inst Subtrop Anim Nutr & Feed, Coll Anim Sci, Guangdong Prov Key Lab Anim Nutr Control, Guangzhou, Guangdong, Peoples R China.

通讯机构： [Li, TJ; Yin, YL] C;Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China.

关键词： *Amino acid;*Autophagy;*Cancer;*FGF21;*IGF-1;*Protein restriction;*mTOR

摘要： Protein restriction without malnutrition is currently an effective nutritional intervention known to prevent diseases and promote health span from yeast to human. Recently, low protein diets are reported to be associated with lowered cancer incidence and mortality risk of cancers in human. In murine models, protein restriction inhibits tumor growth via mTOR signaling pathway. IGF-1, amino acid metabolic programing, FGF21, and autophagy may also serve as potential mechanisms of protein restriction mediated cancer prevention. Together, dietary intervention aimed at reducing protein intake can be beneficial and has the potential to be widely adopted and effective in preventing and treating cancers.

语种： 英文

作者： Ran, Maoliang;Weng, Bo;Cao, Rong;Li, Zhi;Peng, Fuzhi;...

期刊： Reproduction in Domestic Animals,2018年53(6):1375-1385 ISSN：0936-6768

通讯作者： Chen, Bin

作者机构： [Cao, Rong; Gao, Hu; Ran, Maoliang; Weng, Bo; Li, Zhi; Peng, Fuzhi; Luo, Hui; Chen, Bin] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Gao, Hu; Ran, Maoliang; Weng, Bo; Li, Zhi; Peng, Fuzhi; Luo, Hui; Chen, Bin] Hunan Prov Key Lab Genet Improvement Domest Anim, Changsha, Hunan, Peoples R China.

通讯机构： [Chen, Bin] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： apoptosis;miR-26a;PAK2;proliferation;Sertoli cells

摘要： Accumulating reports have demonstrated that microRNAs (miRNAs) participate in regulating the complex processes of animal testis development and spermatogenesis; yet, the mechanisms by which miRNAs regulate spermatogenesis are poorly understood. miR-26a was identified as a miRNA that is differentially expressed among different pig testicular tissue developmental stages in our previous study. In this study, p21 activated kinase 2 (PAK2) gene was determined as one target gene of miR-26a by luciferase reporter assay, and miR-26a repressed the PAK2 mRNA abundance in porcine Sertoli cells. The Cell Counting Kit-8 (CCK8) assay, 5-Ethynyl-2′-deoxyuridine (EdU) assay and annexin V-FITC/PI staining assay results showed that miR-26a overexpression inhibited proliferation and promoted apoptosis in porcine Sertoli cells. These phenomena were similar to the siRNA-mediated knockdown of the PAK2 gene. Taken together, our results demonstrate that miR-26a inhibits proliferation and promotes apoptosis in porcine Sertoli cells by targeting the PAK2 gene, which may be a regulator of porcine spermatogenesis. © 2018 Blackwell Verlag GmbH

语种： 英文

作者： Yan, Qiongxian;Xu, Juanzhi;Wu, Xiaosong*;Su, Dingding;Tan, Zhiliang*

期刊： British Journal of Nutrition,2018年119(10):1119-1132 ISSN：0007-1145

通讯作者： Tan, Zhiliang;Wu, Xiaosong

作者机构： [Yan, Qiongxian; Tan, Zhiliang; Xu, Juanzhi] Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg,Sci Observi, Inst Subtrop Agr,Natl Engn Lab Pollut Control & W, Minist Agr,Hunan Prov Engn Res Ctr Hlth Livestock, Changsha 410125, Hunan, Peoples R China.;[Yan, Qiongxian] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Wu, Xiaosong; Xu, Juanzhi; Su, Dingding] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Wu, XS; Tan, Zhiliang; Wu, Xiaosong] Hunan Coinnovat Ctr Anim Prod Safety CICAPS, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Tan, Zhiliang] C;[Wu, Xiaosong; Wu, XS; Tan, ZL] H;Chinese Acad Sci, CAS Key Lab Agroecol Proc Subtrop Reg,Sci Observi, Inst Subtrop Agr,Natl Engn Lab Pollut Control & W, Minist Agr,Hunan Prov Engn Res Ctr Hlth Livestock, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Hunan Coinnovat Ctr Anim Prod Safety CICAPS, Changsha 410128, Hunan, Peoples R China.

关键词： Placenta;Feed intake restriction;Capillary;Proteomics;Pregnant goats

摘要： A total of twenty-four healthy twin-bearing Liuyang black goats were allocated to two trials. In Trial 1, twelve goats received either the control diet (CG, n 6, 100 % feed) or restricted diet (RG, n 6, 60 % feed of CG) from gestation days 26 to 65 after synchronisation. In Trial 2, the remaining goats were randomly and equally divided into two treatments: CG and RG from days 95 to 125 of gestation. Placental traits, fetal weight, serum parameters, nitric oxide (NO), angiogenesis gene expression and cotyledon proteome were measured at the end of each trial. In early pregnancy, the total and relative weights of placenta, uterine caruncle and cotyledon, as well as fetus, were increased (P<0·05) in RG. The NO content in maternal serum was also increased (P<0·05) in RG. In all, fifty differentially expressed proteins were identified in cotyledon. The up-regulated proteins are related to proliferation and fission of trophoblast cell and the placenta angiogenesis. During the late pregnancy trial, placental weight was increased (P<0·05) in RG, but weight of the fetus was decreased (P<0·05). The capillary density in the cotyledon was also decreased (P<0·01). A total of fifty-eight proteins were differentially expressed in cotyledon. The up-regulated proteins in RG are related to placenta formation, blood flow regulation and embryonic development. These results indicated that feed intake restriction during gestation influenced the placental and fetal development in a stage-dependent manner. These findings have important implications for developing novel nutrient management strategies in goat production. © Copyright The Authors 2018.

语种： 英文

作者： Liu, Zhaojin;Yuan, Yong;Yin, Jie;Li, Tiejun*;Zhang, Hongfu;...

期刊： PROTEIN AND PEPTIDE LETTERS,2018年25(4):405-416 ISSN：0929-8665

通讯作者： Li, Tiejun;He, Jianhua

作者机构： [Liu, Zhaojin; Li, Tiejun; He, Jianhua; Yuan, Yong; Zhang, Hongfu] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Yin, Jie; Li, Tiejun] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.;[Zhang, Hongfu] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing, Peoples R China.;[Liu, Zhaojin] Guangdong Haid Grp Co LTD, Guangzhou, Guangdong, Peoples R China.;[Liu, Zhaojin] Haid Cent Res Inst, Guangzhou, Guangdong, Peoples R China.

通讯机构： [Li, TJ; He, JH] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

关键词： Lysine;proteomics;biochemical parameters;meat quality;transporter;pig.

摘要： Background: Our previous study suggested that short-term lysine restriction improved feed intake and gut microbiota in piglets. Thus, in this study, we further used proteomics technology to investigate the potential mechanism associated with long-term lysine restriction in pigs. In addition, blood biochemical parameters, meat quality, and muscle nutrient transporters were also evaluated in lysine restricted pigs. Results: The results showed that 131 proteins in the liver were markedly altered in response to dietary lysine restriction, with 54 being downregulated and 77 being upregulated, which mainly involved in neurodegenerative diseases, oxidative phosphorylation, and metabolic pathways. In addition, lysine restriction markedly reduced serum BUN, CHOL, and HDL abundances. Dietary lysine restriction enhanced meat color lightness and upregulated SLC7A2 expression. Conclusion: Lysine restriction affected meat quality, blood biochemical parameters, and liver global protein expressions. This study is the first attempt to evaluate the liver protein profile of lysine restricted pigs, and the current results may provide valuable information with respect to better understanding the host response to lysine restriction in pigs.

语种： 英文

作者： Yin, Jie;Li, Yuying;Han, Hui;Chen, Shuai;Gao, Jing;...

期刊： Journal of Pineal Research,2018年65(4):e12524- ISSN：0742-3098

通讯作者： Wu, Xin;Li, Tiejun;Ren, Wenkai

作者机构： [Liu, Gang; Chen, Shuai; Wu, X; Li, Tiejun; Wu, Xin; Yin, Yulong; Han, Hui; Yin, Jie; Li, Yuying; Gao, Jing] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.;[Liu, Gang; Chen, Shuai; Wu, Xin; Li, Tiejun; Yin, Yulong; Han, Hui; Yin, Jie; Li, Yuying; Gao, Jing] Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Changsha, Hunan, Peoples R China.;[Deng, Jinping; Yin, Yulong; Yin, Jie; Ren, Wenkai] South China Agr Univ, Coll Anim Sci, Inst Subtrop Anim Nutr & Feed, Guangdong Prov Key Lab Anim Nutr Control, Guangzhou, Guangdong, Peoples R China.;[Chen, Shuai; Han, Hui; Yin, Jie; Li, Yuying; Gao, Jing] Univ Chinese Acad Sci, Beijing, Peoples R China.;[Yu, Qifang; Huang, Xingguo; Fang, Rejun] Hunan Agr Univ, Dept Anim Sci, Changsha, Hunan, Peoples R China.

通讯机构： [Wu, X; Li, TJ] C;[Ren, Wenkai] S;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.;South China Agr Univ, Coll Anim Sci, Inst Subtrop Anim Nutr & Feed, Guangdong Prov Key Lab Anim Nutr Control, Guangzhou, Guangdong, Peoples R China.

关键词： acetic acid;gut microbiota;lipid metabolism;melatonin;microbiota transplantation

摘要： Melatonin has been shown to improve lipid metabolism and gut microbiota communities in animals and humans; however, it remains to know whether melatonin prevents obesity through gut microbiota. Here, we found that high-fat diet promoted the lipid accumulation and intestinal microbiota dysbiosis in mice, while oral melatonin supplementation alleviated the lipid accumulation and reversed gut microbiota dysbiosis, including the diversity of intestinal microbiota, relative abundances of Bacteroides and Alistipes, and functional profiling of microbial communities, such as energy metabolism, lipid metabolism, and carbohydrate metabolism. Interestingly, melatonin failed to alleviate the high-fat-induced lipid accumulation in antibiotic-treated mice; however, microbiota transplantation from melatonin-treated mice alleviated high-fat diet-induced lipid metabolic disorders. Notably, short-chain fatty acids were decreased in high-fat diet-fed mice, while melatonin treatment improved the production of acetic acid. Correlation analysis found a marked correlation between production of acetic acid and relative abundances of Bacteroides and Alistipes. Importantly, sodium acetate treatment also alleviated high-fat diet-induced lipid metabolic disorders. Taken together, our results suggest that melatonin improves lipid metabolism in high-fat diet-fed mice, and the potential mechanisms may be associated with reprogramming gut microbiota, especially, Bacteroides and Alistipes-mediated acetic acid production. Future studies are needed for patients with metabolic syndrome to fully understand melatonin’s effects on body weight and lipid profiles and the potential mechanism of gut microbiota. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

语种： 英文

作者： Wu, Xin;Gao, Lumin;Zhou, Kai;Li, Xue;Lin, Xue;...

期刊： Journal of Trace Elements in Medicine and Biology,2018年50:566-571 ISSN：0946-672X

通讯作者： Yin, Yulong

作者机构： [Liu, Gang; Gao, Lumin; Zhou, Kai; Li, Xue; Wu, Xin; Wan, Dan; Xiong, Xia; Lin, Xue; Yin, Yulong] Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Minist Agr,Natl Engn Lab Pollut Control & Waste U, Changsha 410125, Hunan, Peoples R China.;[Gao, Lumin; Zhou, Kai; Li, Xue; Wu, Xin; Lin, Xue; Yin, Yulong] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Yin, Yulong] H;Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.

会议名称： Joint 16th International Symposium on Trace Elements in Man and Animals (TEMA) / 12th Conference of the International-Society-for-Trace-Element-Research-in-Humans (ISTERH) / 13th Conference of the Nordic-Trace-Element-Society (NTES)

会议时间： JUN 26-29, 2017

会议地点： St Petersburg, RUSSIA

会议主办单位： [Wu, Xin;Gao, Lumin;Zhou, Kai;Li, Xue;Lin, Xue;Wan, Dan;Xiong, Xia;Liu, Gang;Yin, Yulong] Chinese Acad Sci, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Minist Agr,Natl Engn Lab Pollut Control & Waste U, Changsha 410125, Hunan, Peoples R China.^[Wu, Xin;Gao, Lumin;Zhou, Kai;Li, Xue;Lin, Xue;Yin, Yulong] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.

关键词： Stocking density;Serum;Liver;Trace minerals;Fattening pigs

摘要： Trace minerals are important for balanced nutrition in pigs and to maintain pig growth under high stocking densities. To study the effects of stocking density on serum and liver trace mineral deposition in fattening pigs, 288 conventional pigs (Duroc × Landrace × Large) were selected and assigned to one of three groups: low, medium or high density (8, 16, or 24 pigs, respectively, per 5.2 m × 3.8 m pen). On d 30, one pig per pen was chosen, blood samples were taken, and the pigs were sacrificed; liver and intestinal mucosa samples were obtained from these pigs for trace mineral determination and RT-PCR. The results showed that compared with those of the low-density group, serum Fe, Zn, and Mn concentrations significantly decreased (P < 0.05), while liver Fe and Mn significantly increased in both the medium- and high-density groups (P < 0.05). mRNA expression of ATP7A, ATP7B, FRRS1, and SLC30A3 transporters was significantly upregulated in the liver of the medium-density group, and FRRS1 and SLC1A2 expression in the liver, MT-2b in the jejunal mucosa, and SLC11A2 (DMT1) and FRRS1 in the ileal mucosa were upregulated in the high-density group (P < 0.05). Alternatively, ATX1 expression in the jejunal mucosa of the medium-density group, SLC30A9 in the duodenal and jejunal mucosa, ATX1 in the jejunal mucosa, and MT-2b in the ileal mucosa of the high-density group were downregulated (P < 0.05). These results demonstrated that stocking density affected serum Fe, Zn, and Mn, as well as liver Fe and Mn. Stocking density also affected mRNA expression of trace mineral transporters in both the liver and intestinal mucosa of fattening pigs under the studied conditions. © 2018

语种： 英文

作者： Zhou, Xihong;He, Liuqin;Zuo, Shengnan;Zhang, Yumei;Wan, Dan;...

期刊： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2018年1864(2):488-498 ISSN：0925-4439

通讯作者： Wu, Xin;Liu, Gang;Wu, Canrong

作者机构： [Zhou, Xihong; Huang, Pan; Liu, Gang; Long, Ciming; Wu, X; Liu, G; Wan, Dan; Wu, Xin; Yin, Yulong; He, Liuqin] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Beijing, Peoples R China.;[Zuo, Shengnan; Wu, Canrong] Hunan Univ TCM, Dept Pathogen & Immunol, Changsha 410007, Hunan, Peoples R China.;[Zuo, Shengnan] Hunan Guangxiu Hosp, Clin Lab Dept, Changsha 410000, Hunan, Peoples R China.;[Zhang, Yumei] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Anim Prod Safety, CICAPS, Changsha 410128, Hunan, Peoples R China.;[Zhou, Xihong] China Agr Univ, State Key Lab Anim Nutr, Beijing 100193, Peoples R China.

通讯机构： [Wu, X; Liu, G] C;[Wu, Canrong] H;Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Beijing, Peoples R China.;Hunan Univ TCM, Dept Pathogen & Immunol, Changsha 410007, Hunan, Peoples R China.

关键词： AMP-activated protein kinase;Glutathione;Hypermethylation;Oxidative stress;Serine;Steatosis

摘要： Serine deficiency has been observed in patients with nonalcoholic fatty liver disease (NAFLD). Whether serine supplementation has any beneficial effects on the prevention of NAFLD remains unknown. The present study was conducted to investigate the effects of serine supplementation on hepatic oxidative stress and steatosis and its related mechanisms. Forty male C57BL/6J mice (9 week-old) were randomly assigned into four groups (n = 10) and fed: i) a low-fat diet; ii) a low-fat diet supplemented with 1% (wt:vol) serine; iii) a high-fat (HF) diet; and iv) a HF diet supplemented with 1% serine, respectively. Palmitic acid (PA)-treated primary hepatocytes separated from adult mice were also used to study the effects of serine on oxidative stress. The results showed that serine supplementation increased glucose tolerance and insulin sensitivity, and protected mice from hepatic lipid accumulation, but did not significantly decreased HF diet-induced weight gain. In addition, serine supplementation protected glutathione (GSH) antioxidant system and prevented hypermethylation in the promoters of glutathione synthesis-related genes, while decreasing reactive oxygen species (ROS) in mice fed a HF diet. Moreover, we found that serine supplementation increased phosphorylation and S-glutathionylation of AMP-activated protein kinase α subunit (AMPKα), and decreased ROS, malondialdehyde and triglyceride contents in PA-treated primary hepatocytes. However, while AMPK activity or GSH synthesis was inhibited, the abovementioned effects of serine on PA-treated primary hepatocytes were not observed. Our results suggest that serine supplementation could prevent HF diet-induced oxidative stress and steatosis by epigenetically modulating the expression of glutathione synthesis-related genes and through AMPK activation. © 2017 Elsevier B.V.

语种： 英文

作者： Lin, Xue;Liu, Yilin;Meng, Tiantian;Xie, Chunyan;Wu, Xin*;...

期刊： Journal of Trace Elements in Medicine and Biology,2018年50:518-526 ISSN：0946-672X

通讯作者： Wu, Xin

作者机构： [Meng, Tiantian; Liu, Yilin; Wu, Xin; Lin, Xue; Yin, Yulong; Xie, Chunyan] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Changsha 410125, Hunan, Peoples R China.;[Meng, Tiantian; Wu, Xin; Lin, Xue; Yin, Yulong] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.;[Wu, Xin] Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Wu, Xin] I;Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.

会议名称： Joint 16th International Symposium on Trace Elements in Man and Animals (TEMA) / 12th Conference of the International-Society-for-Trace-Element-Research-in-Humans (ISTERH) / 13th Conference of the Nordic-Trace-Element-Society (NTES)

会议时间： JUN 26-29, 2017

会议地点： St Petersburg, RUSSIA

会议主办单位： [Lin, Xue;Liu, Yilin;Meng, Tiantian;Xie, Chunyan;Wu, Xin;Yin, Yulong] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Hunan Prov Engn Res Ctr Hlth Livestock & Poultry, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Changsha 410125, Hunan, Peoples R China.^[Lin, Xue;Meng, Tiantian;Wu, Xin;Yin, Yulong] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Safety Anim Prod, Changsha 410128, Hunan, Peoples R China.

关键词： Dynamic feeding;Calcium;Zinc;Circadian clock;Oxidative;Laying hens

摘要： The study was conducted to investigate the effects of different circadian calcium feeding regimes on parameters of Zn status and gene expression of circadian clock, calcium transporters and oxidative status in laying hens. In total, 180 of 41-weeks Brown Hy-line laying hens were assigned randomly into three groups, 1-CON group (Control Ca, diets contained 3.4% Ca at both 0730 and 1530 h), 2-HL group (High-low Ca, diets contained 3.6%–3.2% Ca respectively) and 3-LH group (Low-high Ca, diets contained 3.2%–3.6% Ca respectively), which were fed a certain amount of control diet at 0730 h and 1530 h. Blood, tibia, jejunum and kidney samples were collected at 4 h intervals with initial starting at 0800 h after 10 weeks of experiment. Compared with the CON group: 1) the serum zinc in HL group increased at 2000 h, but lower at 1600 h in LH group (P < 0.05). 2) in jejunum, circadian clock genes including CLOCK and BMAL1 expression of HL group were down-regulated at 0000 h and 1600 h, as well as CLOCK, BMAL1, Cry2, Per3 and calcium transporter gene NCX1 in LH group at 2000 h (P < 0.05). 3) in kidney, CLOCK, Cry1, Cry2 and Per3 of LH group were up-regulated at 0400 h, CLOCK at 0000 h as well, while CLOCK at 2000 h were down-regulated (P < 0.05). 4) in kidney, the calcium transporters including PMCA, CaBP and CA of LH group were up-regulated at 0000 h, but PMCA and CaBP of LH group were down-regulated at 0800 h, 1200 h and 1600 h, as well as CA at 1600 h and PMCA at 2000 h of LH group (P < 0.05), and the oxidative gene SOD of the LH group was up-regulated at 0400 h, as well as CAT at 0400 h, SOD and GPX1 at 1200 h in HL group, but SOD at 1600 h and 2000 h, and GPX1 at 1600 h were down-regulated in LH group (P < 0.05). These results demonstrated that the dynamic circadian calcium feeding regime affected circadian rhythms of serum zinc concentration as well as the expression of certain genes related to the circadian clock, calcium transport and antioxidative capacity, and circadian calcium feeding regimes may therefore be considered with regard to improving the calcium usability. © 2018 Elsevier GmbH

语种： 英文

作者： Zhong, Jin-Feng;Wu, Wei-Gao;Tastan, Aseyinjan;Zhang, Xiao-Qing;Wang, Bin;...

期刊： Animal Science Journal,2018年89(4):703-712 ISSN：1344-3941

通讯作者： Fang, Re-Jun

作者机构： [Wu, Wei-Gao; Zhong, Jin-Feng] Hunan Polytech Environm & Biol, Hengyang, Peoples R China.;[Tang, Xiao-Peng; Zhong, Jin-Feng; Fang, Re-Jun; Wang, Bin] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Tang, Xiao-Peng; Zhong, Jin-Feng; Fang, Re-Jun; Wang, Bin] Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Tastan, Aseyinjan] Yili Vocat & Tech Coll, Yining, Peoples R China.;[Zhang, Xiao-Qing] Chinese Acad Agr Sci, Grassland Res Inst, Hohhot, Peoples R China.

通讯机构： [Fang, Re-Jun] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： fattening pig;growth performance;meat quality;Mycobacterium phlei

摘要： Inactivated mycobacterium phlei (M. phlei) is well known for its immune-stimulatory functions in humans and livestock, but less information is available about the influence on meat quality of pigs when used as a feed additive. This study was designed to evaluate the effects of inactivated M. phlei on growth performance as well as meat quality of fattening pigs. A total of 240 cross-bred pigs ([Landrace × Yorkshire] × Duroc) with initial body weight of 80.14 ± 0.29 kg were randomly allocated to five treatments, each of which consisted of eight replicates with 6six pigs per replicate. The basal diet supplemented with five levels of inactivated M. phlei preparations (0, 3.5 × 109 [0.1% w/w], 7 × 109 [0.2%], 1.4 × 1010 [0.4%] or 2.1 × 1010 [0.6%] colony-forming units/kg) was respectively fed to the control group and four treatment groups for 30 days. Adding 0.4% of inactivated M. phlei to diet increased the average daily feed intake and average daily gain of pigs. Importantly, intramuscular fat percentage in the Longissimus dorsi (LD) was increased by feeding diet containing 0.2%, 0.4% and 0.6% of inactivated M. phlei, despite the pH value, drip loss, cooking loss and filter paper fluid uptake not being influenced. Analysis of the fatty acid components showed that some saturated fatty acids were decreased in LD after feeding inactivated M. phlei, but some monounsaturated fat acids (MUFAs) and polyunsaturated fatty acids were increased (PUFAs), which induced the total contents of MUFAs and PUFAs were improved. RT-PCR assay revealed that feeding inactivated M. phlei up-regulated genes implicated in fat metabolism in muscle, including ELOVL6, FASN, SCD1 and H-FABP. This study revealed that feeding inactivated M. phlei not only increased growth performance of fattening pigs, but also improved the meat quality by increasing intramuscular fat content, thus inactivated M. phlei probably has high utilization value in modern pig production. © 2018 Japanese Society of Animal Science

语种： 英文

作者： Wan, Liang;Dong, Linsong;Xiao, Shijun;Han, Zhaofang;Wang, Xiaoqing;...

期刊： JOURNAL OF GENETICS,2018年97(4):887-895 ISSN：0022-1333

通讯作者： Wang, Zhiyong

作者机构： [Wan, Liang; Wang, Xiaoqing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Wan, Liang; Wang, Zhiyong] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266325, Peoples R China.;[Xiao, Shijun; Dong, Linsong; Han, Zhaofang; Wan, Liang; Wang, Zhiyong] Jimei Univ, Minist Agr, Fisheries Coll, Key Lab Hlth Mariculture East China Sea, Xiamen 361021, Peoples R China.

通讯机构： [Wang, Zhiyong] Q;[Wang, Zhiyong] J;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266325, Peoples R China.;Jimei Univ, Minist Agr, Fisheries Coll, Key Lab Hlth Mariculture East China Sea, Xiamen 361021, Peoples R China.

关键词： genomewide association study;extreme phenotypes;large yellow croaker;economic trait;Larimichthys crocea

摘要： A traditional genomewide association study (GWAS) detects genotype–phenotype associations by the vast number of genotyped individuals. This method requires large-scale samples and considerable sequencing costs. Extreme phenotypic sampling proposes make GWAS more cost-efficient and are applied more widely. With extreme phenotypic sampling, we performed a GWAS for n-3 highly unsaturated fatty acids (HUFA) and eviscerated weight (EW) traits in the large yellow croaker population. Of the 32,249 and 29,748 detected SNPs for the two traits, three candidate regions were found in each trait. Three candidate regions associated with HUFA were known near genes on chromosomes 4 and 11, and three candidate regions were on chromosome 6, and 15 for the EW trait. By combing through our GWAS results and the biological functional analysis of the genes, we suggest that the FABP, DGAT, ATP8B1, FAF2 and CERS2 genes, as well as the IGF2, BORA, CYP1A1, GRTP1 and HOX genes are promising candidate genes for n-3 HUFA and EW, respectively, in the large yellow croaker. Moreover, compared with the different numbers of the extreme phenotypic sampling, we conclude that 60% of the extreme phenotypic subsample can obtain a similar result as GWAS with whole phenotypes. Thus, extreme phenotypic sampling could save 40% of the cost for genotyping and DNA extraction without loss of the candidate regions and functional genes. Our study may provide a basis for further genomic breeding and a reference for others who want to perform GWAS with extreme phenotypes. © 2018, Indian Academy of Sciences.

语种： 英文

作者： Jiang, Qian;Liu, Gang;Chen, Jiashun;Yao, Kang*;Yin, Yulong

期刊： Cellular Physiology and Biochemistry,2018年48(6):2441-2455 ISSN：1015-8987

通讯作者： Yao, Kang

作者机构： [Liu, Gang; Chen, Jiashun; Yao, Kang; Yin, Yulong; Jiang, Qian] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha, Hunan, Peoples R China.;[Jiang, Qian] Univ Chinese Acad Sci, Beijing, Peoples R China.;[Liu, Gang; Yao, Kang; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Soil Mol Ecol Sect, Taoyuan Agroecosyst Res Stn, Changsha, Hunan, Peoples R China.;[Liu, Gang; Chen, Jiashun; Yao, Kang; Yin, Yulong] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.;[Yao, Kang] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.

通讯机构： [Yao, Kang] C;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.

关键词： Endoplasmic reticulum stress;IPEC-J2;GRP78;P53;Inflammation;LPS

摘要： Background/Aims: Lipopolysaccharides (LPSs) act as virulence factors that trigger intestinal inflammation and thereby compromise the production of pigs worldwide. Intestinal diseases and dysfunction have been attributed to endoplasmic reticulum stress (ERS) and the subsequent apoptosis of intestinal epithelial cells. Therefore It is important to explore whether LPSs aggravate ERS-mediated apoptosis of intestinal epithelial cells. Methods: ERS and inflammation models were established in porcine cell line J2 (IPEC-J2) and the cells were treated with tunicamycin or LPS at specific times. The expression of marker proteins was determined by western blot and immunofluorescence. Possible crosstalk between proteins was analyzed by co-immunoprecipitation. Small interfering RNA transfection was employed to verify the mechanisms. Results: We found that Escherichia coli-derived LPS aggravated ERS and ERS-mediated apoptosis in ERS-responsive IPEC-J2 cells. The crosstalk between nuclear glucose-regulated protein 78 (GRP78) and tumor protein 53 (p53) was verified to trigger this LPS-aggravated apoptosis of ERS-responsive intestinal cells. Conclusion: This novel finding implies that intestinal malfunctions might solely originate from the effects of Gram-negative bacteria on ERS-responsive intestinal cells. The regulation of ERS signaling (especially the crosstalk between nuclear GRP78 and p53) in ERS-responsive/rapidly growing intestines may help intestinal cells survive from Gram-negative bacterial infections. © 2018 The Author(s).

语种： 英文

作者： Ji, Shengyue;Liu, Ming;Zhang, Yuping;Zhang, Hongfu*

期刊： BioMed Research International,2018年2018:7809512 ISSN：2314-6133

通讯作者： Zhang, Hongfu

作者机构： [Liu, Ming; Zhang, Yuping; Ji, Shengyue; Zhang, Hongfu] Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Nutr, Beijing 100193, Peoples R China.;[Liu, Ming; Zhang, Hongfu] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Zhang, Yuping] Univ Liege, Dept AgroBioChem, Precis Livestock & Nutr Unit, Passage Deportes 2, B-5030 Gembloux, Belgium.

通讯机构： [Zhang, Hongfu] C;[Zhang, Hongfu] H;Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Nutr, Beijing 100193, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

摘要： LEF-1/wnt10b is one of the most important signaling pathways regulating mammary gland growth and development and is also a potential target for molecular breeding. In this work, 1-deoxynojirimycin (DNJ), a natural alkaloid extracted from plant mulberry or microorganism, was found to have a positive activity in primary breast epithelial cell growth of dairy goats. The findings showed that, compared to the control, 6 μM DNJ in the DMEM/F12 medium in vitro greatly improved the density of dairy goat breast epithelial cell and significantly increased the LEF-1 mRNA level (P<0.01) and thus enhanced cell growth. In addition, DNJ displayed a similar function in alleviating the growth suppression of epithelial cell and the decrease of LEF-1 mRNA level resulting from lentiviral-mediated LEF-1 knockdown. Simultaneously, no significant change of the mRNA levels of IGF-1 and Fgf10, the other two key regulators in mammary gland growth and development, could be detected. Furthermore, the mammary duct of DNJ-fed mouse illustrated a better development accompanied with a higher LET-1 mRNA level than that of the control. In conclusion, DNJ could improve breast epithelial cell growth through upregulating LEF-1 expression, which supplied a new means in studying mammary gland growth and development. © 2018 Shengyue Ji et al.

语种： 英文

作者： Xu, Kang;Liu, Gang*;Fu, Chenxing

期刊： Oxidative Medicine and Cellular Longevity,2018年2018:1054797 ISSN：1942-0900

通讯作者： Liu, Gang

作者机构： [Liu, Gang; Xu, Kang] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Agroecol Proc Subtrop Reg, Lab Anim Nutr Physiol & Metab Proc,Inst Subtrop A, Changsha 410125, Hunan, Peoples R China.;[Fu, Chenxing] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Fu, Chenxing] Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410128, Hunan, Peoples R China.;[Fu, Chenxing] Hunan Collaborat Innovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Liu, Gang] C;Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Agroecol Proc Subtrop Reg, Lab Anim Nutr Physiol & Metab Proc,Inst Subtrop A, Changsha 410125, Hunan, Peoples R China.

摘要： The placenta plays a vital role in fetal development during pregnancy. Dysfunction of the placenta can be caused by oxidative stress and can lead to abnormal fetal development. Preventing oxidative stress of the placenta is thus an important measure to ensure positive birth outcomes. Research shows that tryptophan and its metabolites can efficiently clean free radicals (including the reactive oxygen species and activated chlorine). Consequently, tryptophan and its metabolites are suggested to act as potent antioxidants in the placenta. However, the mechanism of these antioxidant properties in the placenta is still unknown. In this review, we summarize research on the antioxidant properties of tryptophan, tryptophan metabolites, and metabolic enzymes. Two predicted mechanisms of tryptophan's antioxidant properties are discussed. (1) Tryptophan could activate the phosphorylation of p62 after the activation of mTORC1; phosphorylated p62 then uncouples the interaction between Nrf2 and Keap1, and activated Nrf2 enters the nucleus to induce expressions of antioxidant proteins, thus improving cellular antioxidation. (2) 3-Hydroxyanthranilic acid, a tryptophan kynurenine pathway metabolite, changes conformation of Keap1, inducing the dissociation of Nrf2 and Keap1, activating Nrf2 to enter the nucleus and induce expressions of antioxidant proteins (such as HO-1), thereby enhancing cellular antioxidant capacity. These mechanisms may enrich the theory of how to apply tryptophan as an antioxidant during pregnancy, providing technical support for its use in regulating the pregnancy's redox status and enriching our understanding of amino acids' nutritional value. Copyright © 2018 Kang Xu et al.

语种： 英文

作者： Azad, Md. Abul Kalam;Bin, Peng;Liu, Gang*;Fang, Jun;Li, Tiejun;...

期刊： FOOD & FUNCTION,2018年9(11):5843-5854 ISSN：2042-6496

通讯作者： Liu, Gang

作者机构： [Liu, Gang; Bin, Peng; Li, Tiejun; Yin, Yulong; Azad, Md. Abul Kalam] Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.;[Azad, Md. Abul Kalam] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Liu, Gang; Fang, Jun] Hunan Agr Univ, Coll Anim Sci & Technol, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Yin, Yulong] Academician Workstn Hunan Baodong Farming Co Ltd, Changsha 422001, Hunan, Peoples R China.

通讯机构： [Liu, Gang] C;[Liu, Gang] H;Chinese Acad Sci, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Key Lab Agroecol Proc Subtrop Reg,Inst Subtrop Ag, Changsha 410125, Hunan, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

摘要： Maternal dietary supplementation during gestation and lactation improves the health of piglets. The purpose of this study was to determine the effect of different levels of methionine (Met) supplementation in the sows' diet during late gestation and lactation on piglets. Thirty sows were randomly divided into three groups and fed the following diets from day 90 of gestation to day 21 of lactation: (a) control group (a basal diet containing 0.36% Met), (b) 0.48% Met group (a basal diet with additional 0.12% Met), and (c) 0.60% Met group (a basal diet with additional 0.24% Met). On day 21 after farrowing, piglets of average body weight (n = 10 per group) were selected for sample collection. The results showed that the 0.48% Met and 0.60% Met diets significantly lowered the malondialdehyde content in the piglets' serum (P < 0.05). In addition, the glutathione peroxidase content was significantly increased in the 0.48% Met group (P < 0.05) and the total glutathione content was significantly reduced in the 0.60% Met group (P < 0.05) compared to the control group. Furthermore, Met supplementation of the sows' diet was associated with alterations in 37 plasma metabolites in the piglets. In the piglets' intestinal microbiota, the relative abundances of Phascolarctobacterium and Bacteroidetes in the 0.48% Met group were higher than those in the other two groups (P < 0.05). Our results suggest that a diet including 0.48% Met during late gestation and lactation can maintain the health of piglets by increasing the antioxidant capacity and changing the intestinal microbiota composition, but a higher level of Met supplementation may increase the potential risk to piglets. © 2018 The Royal Society of Chemistry.

语种： 英文

作者： Yin, Jie;Li, Yuying;Han, Hui;Liu, Zhaojin;Zeng, Xiangfang;...

期刊： FOOD & FUNCTION,2018年9(8):4153-4163 ISSN：2042-6496

通讯作者： Li, Tiejun;Yin, Yulong

作者机构： [Han, Hui; Yin, Jie; Li, Yuying; Li, Tiejun; Yin, YL; Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr Physiol & Metab Proc, Changsha 410125, Hunan, Peoples R China.;[Han, Hui; Yin, Jie; Li, Yuying] Univ Chinese Acad Sci, Beijing 100039, Peoples R China.;[Liu, Zhaojin] Hunan Agr Univ, Dept Anim Sci, Changsha 410125, Hunan, Peoples R China.;[Zeng, Xiangfang] Chinese Agr Univ, Coll Anim Sci & Technol, Beijing, Peoples R China.;[Li, Tiejun; Yin, YL; Yin, Yulong] Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.

通讯机构： [Li, TJ; Yin, YL] C;[Li, TJ; Yin, YL] N;[Li, TJ; Yin, YL] H;Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr Physiol & Metab Proc, Changsha 410125, Hunan, Peoples R China.;Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha 410125, Hunan, Peoples R China.

摘要： Lysine is a common limiting amino acid in human and animal diets and plays an important role in cell proliferation and metabolism. Our previous study suggested that short-term lysine restriction improved feed intake. Herein, we further investigated the long-term effects of lysine restriction on intestinal microbial communities and metabolic profiles in pigs by 16S rDNA sequencing and metabolomic analysis. The results showed that 30% lysine limitation increased the feed intake in piglets but not in growing and finishing pigs. Microbiota sequencing was conducted, and we identified 12 differentiated microbes at the genus level in response to dietary lysine restriction. Metagenomic predictions by PICRUSt suggested that the altered microbiota was mainly involved in the host metabolism, especially in amino acid metabolism. Intestinal metabolomic study identified 20 differentiated metabolites in finishing pigs, which were mapped into 5 metabolic pathways including glutamine and glutamate metabolism, taurine metabolism, alanine, aspartate and glutamate metabolism, vitamin B6 metabolism, and pentose and glucoronate metabolism. Determination of serum amino acids and intestinal amino acid transporters further validated the results obtained from PICRUSt and metabolomic analyses, which showed that long-term lysine restriction influenced amino acid metabolism. Phosphorylation of mTOR in the intestine was not altered in lysine-restricted pigs, whereas lysine limitation markedly inhibited the AMPK signaling. In conclusion, long-term lysine restriction from piglets to finishing pigs affected the amino acid metabolism, which might be associated with gut microbiota and AMPK signaling. © The Royal Society of Chemistry.

语种： 英文

作者： Guan, Guiping;Lan, Shile*

期刊： BioMed Research International,2018年2018:1290179 ISSN：2314-6133

通讯作者： Lan, Shile

作者机构： [Lan, Shile] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Lan, Shile] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

摘要： The global incidence of inflammatory bowel disease (IBD), a group of chronic gastrointestinal disorders, has been rising. The preponderance of evidence demonstrates that oxidative stress (OS) performs a critical function in the onset of IBD and the manner of its development. The purpose of this review is to outline the generation of reactive oxygen species and antioxidant defense mechanisms in the gastrointestinal tract and the role played by OS in marking the onset and development of IBD. Furthermore, the review demonstrates the various ways through which OS is related to genetic susceptibility and the mucosal immune response. The experimental results suggest that certain therapeutic regimens for IBD could have a favorable impact by scavenging free radicals, reducing cytokine and prooxidative enzyme concentrations, and improving the antioxidative capabilities of cells. However, antioxidative activity characterized by a high level of specificity may be fundamental for the development of clinical therapies and for relapsing IBD patients. Therefore, additional research is required to clarify the ways through which OS is related to the pathogenesis and progression of IBD. © 2018 Guiping Guan and Shile Lan.

语种： 英文

作者： Yin, Jie;Han, Hui;Li, Yuying;Liu, Zhaojin;Zhao, Yurong;...

期刊： Cellular Physiology and Biochemistry,2018年44(5):1749-1761 ISSN：1015-8987

通讯作者： Li, Tiejun;Yin, Yulong

作者机构： [Liu, Gang; Li, Chunyong; Wu, Xin; Li, Tiejun; Yin, Yulong; Han, Hui; Yin, Jie; Li, Yuying; Ren, Wenkai; Wu, Fei] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Inst Subtrop Agr,Minist Agr,Hunan Prov Engn Res C, Changsha, Hunan, Peoples R China.;[Han, Hui; Yin, Jie; Li, Yuying; Ren, Wenkai; Wu, Fei] Univ Chinese Acad Sci, Beijing, Peoples R China.;[Zheng, Jie; Huang, Xingguo; Liu, Zhaojin; Fang, Rejun; Zhao, Yurong] Hunan Agr Univ, Dept Anim Sci, Changsha, Hunan, Peoples R China.;[Liu, Gang; Wu, Xin; Huang, Xingguo; Li, Tiejun; Yin, Yulong; Fang, Rejun; Zhao, Yurong] Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Sun, Liping; Wang, Kai] Chinese Acad Agr Sci, Inst Apicultural Res, Beijing, Peoples R China.

通讯机构： [Li, TJ; Yin, YL] C;Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China.

关键词： Feed intake;Lysine restriction;Microbiome;Piglet

摘要： Background/Aims: Our previous reports suggested that dietary supplementation with lysine influenced intestinal absorption and metabolism of amino acids. In this study, we further investigated the effect of lysine restriction (30%) on feed intake and we also tested the hypothesis that gut microbiome contributed to the potential mechanism of lysine restriction-mediated feeding behavior. Here, we profiled gut microbial communities by sequencing 16S ribosomal ribonucleic acid (rRNA) genes from gut samples as well as growth performance, serum hormones, and intestinal lysine transport in a piglet model. Results: Piglets preferred to the lysine restricted diet when giving three diets and the feed intake was markedly higher in the lysine-restricted group than that in the control group. Altered hormones (leptin, CCK, and ghrelin) might contribute to the feeding behavior caused by lysine restriction. Meanwhile, lysine transporting ability (SLC7A1 and SLC7A2 expression, intestinal electrophysiological changes, and amino acid pool in mesenteric vein) was decreased in response to lysine restriction. Through deep sequencing of bacterial rRNA markers, we observed that bacterial diversity was enhanced in the lysine-restricted group (Shannon H, PD, and Chao1). At the phylum level, lysine restriction enhanced gut Actinobacteria, Saccharibacteria, and Synergistetes abundances. At the family level, Moraxellaceae, Halomonadaceae, Shewanellaceae, Corynebacteriaceae, Bacillaceae, Comamonadaceae, Microbacteriaceae, Caulobacteraceae, and Synergistaceae abundances were increased in response to lysine restriction. Predictive functional profiling of microbial communities by PICRUSt also confirmed that dietary lysine restriction affected gut microbiome, which might further mediate amino acid metabolism, membrane transport, and endocrine system. Conclusion: Our results indicated that lysine restriction inhibited intestinal lysine transport and promoted feed intake, which might be associated with gut microbiome. © 2017 The Author(s). Published by S. Karger AG, Basel.

语种： 英文

作者： Tang, Xiaopeng;Liu, Bo;Wang, Xiangrong;Yu, Qifang;Fang, Rejun*

期刊： International Journal of Molecular Sciences,2018年19(3) ISSN：1422-0067

通讯作者： Fang, Rejun

作者机构： [Liu, Bo; Fang, Rejun; Yu, Qifang; Tang, Xiaopeng; Wang, Xiangrong] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Fang, Rejun; Wang, Xiangrong] Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Fang, Rejun] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.

关键词： antioxidant;apoptosis;epidermal growth factor;lipopolysaccharides (LPS);oxidative stress

摘要： The epidermal growth factor (EGF) has been widely used for protection of stress-induced intestinal mucosa dysfunction. However, whether EGF would alleviate oxidative injury and reduce apoptosis in porcine intestine is not yet known. Therefore, the aim of this study was to investigate the effect of EGF on lipopolysaccharides (LPS)-induced induction of oxidative stress and ensuing apoptosis in the porcine intestinal epithelial cell line, IPEC-J2. The present study showed that EGF significantly increased cell viability and decreased the LPS-induced induction of apoptosis, dehydrogenase (LDH) release and malonaldehyde (MDA) production. EGF also (i) decreased expression of the pro-apoptotic genes Fas, Bax, Cascase-3, Cascase-8, Cascase-9, and proteins such as P53, Fas, Bax, Caspase3; (ii) increased antiapoptotic protein B-cell lymphoma 2 (Bcl2) expression; (iii) increased mRNA levels of the nuclear factor erythroid 2-related factor 2 (Nrf2) related genes Nrf2, manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GSH-Px), heme oxygenase (HO-1) and quinone oxidoreductase (NQO1); (iv) protein level of Nrf2-realeted proteins Nrf2, HO-1, NQO1; and (v) total antioxidant capacity (T-AOC), CAT, SOD, GSH-Px concentrations. Collectively, our results indicated that EGF enhanced Nrf2 protein expression, and upregulated the expression of phase II metabolizing enzymes (such as HO-1 and NQO1) and antioxidative enzymes (SOD, CAT and GSH-Px) to alleviate oxidative injury, and then protect IPEC-J2 cells from apoptosis induced by LPS.

语种： 英文

作者： Wang, Ronghua;Li, Yaoguo;Zhou, Zhiyu;Liu, Qiaolin;Zeng, Lingbing;...

期刊： Gene,2018年648:5-11 ISSN：0378-1119

通讯作者： Xiao, Tiaoyi

作者机构： [Xiao, Tiaoyi; Zeng, Lingbing; Zhou, Zhiyu; Liu, Qiaolin; Wang, Ronghua; Li, Yaoguo] Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.;[Wang, Ronghua] Hunan Univ Arts & Sci, Coll Life & Environm Sci, Changde 415000, Hunan, Peoples R China.;[Xiao, Tiaoyi; Liu, Qiaolin; Li, Yaoguo] Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Hunan, Peoples R China.;[Zeng, Lingbing] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China.

通讯机构： [Xiao, Tiaoyi] H;Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Hunan, Peoples R China.

关键词： Squaliobarbus curriculus;Tissue;Ctenopharyngodon idellus kidney cell;Type I IFN;Viral infection

摘要： The barbel chub Squaliobarbus curriculus is an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) from Squaliobarbus curriculus, designated as ScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA of ScIRF3 contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. The ScIRF3 protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain. Phylogenetic analysis showed that ScIRF3 was closely clustered with IRF3s from Carassius auratus and Ctenopharyngodon idellus. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScIRF3 in Squaliobarbus curriculus were the highest in the spleen and lowest in the muscle. After GCRV infection, expression levels of both ScIRF3 and type I interferon (IFN) were initially up-regulated and subsequently down-regulated in the spleen and intestine. Correlation analysis showed that the expression level of type I IFN is significantly positively correlated with that of ScIRF3 (Pearson correlation coefficient: 0.883, P: 0.004) in the intestine. The expression level of type I IFN was also significantly up-regulated and the GCRV titer was significantly decreased (P <.05) in GCRV-infected ScIRF3-overexpressing Ctenopharyngodon idellus kidney cells. These results indicate that ScIRF3 may play a role in the type I IFN immune response against GCRV in Squaliobarbus curriculus and can also inhibit GCRV replication in Ctenopharyngodon idellus kidney cells. © 2018 Elsevier B.V.

语种： 英文