通讯机构：
[Tang, ZH ] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;Hunan Engn Technol Res Ctr Rapeseed Oil Nutr Hlth, Changsha 410128, Peoples R China.
关键词：
deep eutectic solvent;Bletilla striata polysaccharide;response surface methodology;antioxidant activity
摘要：
Taking the extraction yield of Bletilla striata polysaccharide (BSP) as the index and taking the type of deep eutectic solvents (DESs), extraction time, extraction temperature, DES water content, and solid-liquid ratio as the investigation factors, single-factor and Box-Behnken response surface tests were carried out to optimize the extraction process of BSP. Thus, the antioxidant activity of BSP on DPPH radicals, ABTS radicals and ferric reducing antioxidant power were determined. The results showed that the most suitable deep eutectic solvent was DES-2, namely choline chloride-urea. The optimal extraction conditions for BSP were an extraction time of 47 min, extraction temperature of 78 °C, water content of 35%, and solid-liquid ratio of 1:25. Under this optimized condition, the extraction yield of BSP was able to reach (558.90 ± 8.83) mg/g, and recycling studies indicated the good cycle stability of the DES. Antioxidant results showed that BSP had superior antioxidant activity and had a dose-response relationship with drug concentration. Compared with Bletilla striata polysaccharide obtained via conventional hot water extraction (BSP-W), the extraction yield of BSP obtained through this method (BSP-2) increased by 36.77%, the scavenging activity of DPPH radicals increased by 24.99%, the scavenging activity of ABTS radicals increased by 41.16%, and the ferric reducing antioxidant power increased by 49.19%. Therefore, DESs as new green reagents and BSP extracted with DESs not only had a high yield but also had strong antioxidant activity.
摘要：
Yellow glutinous rice wine is a traditional Chinese beverage created by soaking, boiling, and fermenting glutinous rice. The majority of current studies on the flavor of yellow glutinous rice wine are based on instrumental analysis, with sensory analysis being overlooked. In this study, 36 volatile chemicals in the fermentation process of yellow wine were annotated by GC-MS and then an OPLS-DA model was built to screen out 13 distinctive substances (VIP > 1, p < 0.01). The relative odor activity value (ROAV) was calculated using the threshold values of these chemicals and 10 substances, including alcohols, esters, and aldehydes, were found as key contributors to the overall flavor of yellow wine. Following that, consumers quantified the sensory descriptors of yellow wine using rate-all-that-apply (RATA), and correspondence analysis revealed three groups of characteristic flavors and odors. Alcohols and esters were found to be key producers of flowery and fruity scents in yellow wine, according to correlation analysis. We discovered two alcohols that are rarely found in yellow wine: [R,R]-2,3-butanediol and 1-phenylethanol. The former was found to be favorably connected with wine scent and pungent odor, and its specific effect on flavor should be researched further.
摘要：
Pantoea dispersa is a Gram-negative bacterium that exists in a variety of environments and has potential in many commercial and agricultural applications, such as biotechnology, environmental protection, soil bioremediation, and plant growth stimulation. However, P. dispersa is also a harmful pathogen to both humans and plants. This "double-edged sword" phenomenon is not uncommon in nature. To ensure survival, microorganisms respond to both environmental and biological stimuli, which could be beneficial or detrimental to other species. Therefore, to harness the full potential of P. dispersa, while minimizing potential harm, it is imperative to unravel its genetic makeup, understand its ecological interactions and underlying mechanisms. This review aims to provide a comprehensive and up-to-date overview of the genetic and biological characteristics of P. dispersa, in addition to potential impacts on plants and humans, as well as to provide insights into potential applications.
摘要：
Herein, an ingenious Halbach ring-mediated magnetic filter (HRMF) for capture, sterilization, and detection of foodborne pathogenic bacteria is developed. A composite material with magnetic nanoparticles (MNP) as the core and coated with polydopamine (PDA) and polyethylenimine (PEI) is synthesized, namely MNP@PDA@PEI. Under a Halbach ring, MNP@PDA@PEI can form chains to generate HRMF. Due to the positively charged of PEI, the HRMF could capture the negatively charged bacteria from solution via electrostatic adsorption. Within the HRMF, sterilization performance could be achieved due to the photothermal effect of MNP@PDA@PEI after 5 min of near-infrared light irradiation. More importantly, the number of bacteria could be calculated by bioluminescence method for measuring ATP, which are released from bacteria after photothermal sterilization. Listeria monocytogenes is chosen as a typical bacterium to evaluate the function of HRMF. The time of whole process has been greatly reduced to 38 min. The capture efficiency is 98.63%, and sterilization rate can achieve 99.96% in real samples. The limit of detection is 10.04 CFU/mL, representing a 10-fold decrease compared with bioluminescence method alone. The proposed platform might open a new way for on-site real-time detection of food borne pathogens, and hold potential to nondestructive sterilization in the food processing industry.
通讯机构：
[Zeng, MM ] J;Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China.
关键词：
Galanga;Maillard reaction;Free radical -centered path;Quantum chemical computation
摘要：
The simultaneous alleviating effect of Rhizoma kaempferiae and kaempferol on accumulation of acrylamide and methylimidazole in cookies was explored. Electron paramagnetic resonance (EPR) and density functional theory (DFT) were used to investigate the potential mechanism relating to the quenching of free radicals and elimination of carbonyl intermediates. The maximum inhibition rates for acrylamide and methylimidazole were 52% and 70% (15 g/kg RK). EPR experiments showed that the HO center dot and carbon-centered free radicals, critical for the production of acrylamide and methylimidazole, were reduced by RK and kaempferol in a dose-dependent manner. DFT indicated that the 1-OH group of kaempferol was the most important and quenched HO center dot mainly via H-atom Transfer. The active carbonyl intermediates, acrylaldehyde, glyoxal, and methylglyoxal can also be reduced by RK and kaempferol, which may result from the quenching of free radicals. On the basis of these results, we suggested that RK and kaempferol exerts simultaneous alleviating effect for accumulation of acrylamide and methylimidazole by blocking free radicals-centered path and reducing active carbonyl intermediate.
通讯机构：
[Xiaofeng Tan] A;[Si Qin] L;Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory of Non-Wood Forest Products of State Forestry Administration, College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>Laboratory of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.
通讯机构：
[Zhonghua Liu] K;Key Laboratory of Tea Science of Ministry of Education, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture, Hunan Agricultural University, Changsha 410128, China
摘要：
Aging-associated cognitive dysfunction has a great influence on the lifespan and healthspan of the elderly. Theaflavins (TFs), a mixture of ingredients formed from enzymatic oxidation of catechins during the manufacture of tea, have a positive contribution to the qualities and antiaging activities of black tea. However, the role of TFs in mitigating aging-induced cognitive dysfunction and the underlying mechanism remains largely unknown. Here, we find that TFs effectively improve behavioral impairment via the microbiota-gut-brain axis: TFs maintain gut homeostasis by improving antioxidant ability, strengthening the immune response, increasing the expression of tight junction proteins, restructuring the gut microbiota, and altering core microbiota metabolites, i.e., short-chain fatty acids and essential amino acids (SCFAs and AAs), and upregulating brain neurotrophic factors. Removing the gut microbiota with antibiotics partly abolishes the neuroprotective effects of TFs. Besides, correlation analysis indicates that the decrease in gut microbiota, such as Bacteroidetes and Lachnospiraceae, and the increase in microbiota metabolites' levels are positively correlated with behavioral improvements. Taken together, our findings reveal a potential role of TFs in mitigating aging-driven cognitive dysfunction via the microbiota-gut-brain axis. The intake of TFs can be translated into a novel dietary intervention approach against aging-induced cognitive decline.
期刊：
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM,2023年108(6):1415-1424 ISSN：0021-972X
通讯作者：
Minghai Hu<&wdkj&>Dongbo Liu
作者机构：
[Wu, Ruiyu; Liu, Dongbo; Zhou, Jiali; Kang, Xincong; Yang, Xiao] Hunan Agr Univ, Hort Coll, 1 Nongda Rd, Changsha 410128, Peoples R China.;[Liu, Dongbo; Yang, Xiao] Clin Res Ctr, State Key Lab Subhlth Intervent Technol, Changsha 410128, Peoples R China.;[Yang, Xiao] Changsha Commerce & Tourism Coll, Nutr & Food Catering, Changsha 410116, Peoples R China.;[Zhou, Jiali] Dept Shizi Mt Primary Care, Changsha 410007, Peoples R China.;[Huang, Bi; Shao, Huige] Changsha Cent Hosp, Endocrinol Dept, Changsha 410004, Peoples R China.
通讯机构：
[Minghai Hu] D;[Dongbo Liu] H;Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University , Changsha 410013 , China<&wdkj&>Horticulture College, Hunan Agricultural University , Changsha 410128 , China<&wdkj&>Clinical Research Center, State Key Laboratory of Subhealth Intervention Technology , Changsha 410128 , China<&wdkj&>College of Food Science and Technology, Hunan Agricultural University , Changsha 410128 , China
摘要：
CONTEXT: The 2021 consensus report on the definition and interpretation of remission of type 2 diabetes (T2D) has been released. Although intermittent fasting diets (IF) are becoming very popular, no studies have investigated their benefit in diabetes remission. OBJECTIVE: The present study examined the effectiveness of IF in diabetes remission and potential remission durability. METHODS: Participants between ages 38 and 72 years with a duration of T2D of 1 to 11 years, a body mass index (BMI) of 19.1 to 30.4, 66.7% male, and antidiabetic agent use and/or insulin injection were randomly allocated at a ratio of 1:1 to the Chinese Medical Nutrition Therapy (CMNT) or control group. The primary outcome was diabetes remission, defined as a stable glycated hemoglobin A1c (HbA1c) level of less than 48 mmol/mol (< 6.5%) for at least 3 months after discontinuing all antidiabetic medications. The secondary outcomes included HbA1c level, fasting blood glucose level, blood pressure, weight, quality of life, and medication costs. We conducted a 12-month follow-up to assess the continuation of remission. RESULTS: On completing the 3-month intervention plus 3-month follow-up, 47.2% (17/36) of participants achieved diabetes remission in the CMNT group, whereas only 2.8% (1/36) of individuals achieved remission in the control group (odds ratio 31.32; 95% CI, 2.39-121.07; P < 0.0001). The mean body weight of participants in the CMNT group was reduced by 5.93 kg (SD 2.47) compared to 0.27 kg (1.43) in the control group. After the 12-month follow-up, 44.4% (16/36) of the participants achieved sustained remission, with an HbA1c level of 6.33% (SD 0.87). The medication costs of the CMNT group were 77.22% lower than those of the control group (60.4/month vs 265.1/month). CONCLUSION: This study demonstrated the clinical efficacy of CMNT in achieving diabetes remission for at least 1 year.
摘要：
High glucose levels can lead to the apoptosis of islet β cells, while autophagy can provide cytoprotection and promote autophagic cell death. Vitamin B12, a water-soluble B vitamin, has been shown to regulate insulin secretion and increase insulin sensitivity. However, the precise mechanism of action remains unclear. In this study, we investigated the influence of vitamin B12 on high glucose-induced apoptosis and autophagy in RIN-m5F cells to elucidate how vitamin B12 modulates insulin release. Our results demonstrate that exposure to 45 mM glucose led to a significant increase in the apoptosis rate of RIN-m5F cells. The treatment with vitamin B12 reduced the apoptosis rate and increased the number of autophagosomes. Moreover, vitamin B12 increased the ratio of microtubule-associated protein 1 light chain 3 beta to microtubule-associated protein 1 light chain 3 alpha (LC3-II/LC3-I), while decreasing the amount of sequestosome 1 (p62) and inhibiting the phosphorylation of p70 ribosomal protein S6 kinase (p70S6K) under both normal- and high-glucose conditions. The additional experiments revealed that vitamin B12 inhibited high glucose-induced apoptosis. Notably, this protective effect was attenuated when the autophagy inhibitor 3-methyladenine was introduced. Our findings suggest that vitamin B12 protects islet β cells against apoptosis induced by high glucose levels, possibly by inducing autophagy.
期刊：
FRONTIERS IN NUTRITION,2023年9:1111880 ISSN：2296-861X
作者机构：
[Deng, Fangming; Wang, Zengguang; Zhao, Lingyan; Zhang, Yixuan] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Peoples R China.;[Chen, Chengcheng] Hunan Guotai Foods Co Ltd, Yueyang, Hunan, Peoples R China.;[Fan, Shichao] Junjie Food Technol Co Ltd, Shaoyang, Peoples R China.
关键词：
bacteriocin;Lactic acid bacteria;Biological characteristics;Separation and purification;Antimicrobial activity
摘要：
In this study, screening bacteriocin-producing strains from 2,000 plant-derived strains by agar well diffusion method was conducted. The corresponding produced bacteriocin was purified and identified by Sephadex gel chromatography, reversed-phase high-performance liquid chromatography (RP-HPLC), and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Meanwhile, the biological characteristics of bacteriocin were investigated. The targeted strain W3-2 was obtained and identified as Lactobacillus plantarum by morphological observation and 16S rRNA gene sequence analysis. Correspondingly, a novel bacteriocin (named plantaricin W3-2) produced by L. plantarum W3-2 with a molecular weight of 618.26 Da, and an amino acid sequence of AVEEE was separated, purified by Sephadex gel chromatography and RP-HPLC, and identified by LC-MS/MS. Further characteristics analysis displayed that plantaricin W3-2 had good thermal, pH stability, and broad-spectrum antimicrobial ability. In conclusion, plantaricin W3-2 can be used as a new food preservative.
关键词：
hydrocolloids;chemical model system;heterocyclic amines;advanced glycation end products;grilled beef patties
摘要：
Effectively inhibiting the formation of heterocyclic amines (HAs) and advanced glycation end products (AGEs) is crucial to human health. In the present study, chemical model systems were used to evaluate the inhibitory effects of seven hydrocolloids on HA and AGE formation. The results showed that hydrocolloids effectively inhibited the formation of two major AGEs. However, their inhibitory action against HA formation showed unexpected results, wherein alginic acid, carrageenan and konjac glucomannan promoted the formation of 2-Amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP), harmane, norharmane and 2-amino-3,8-dimethyl-imidazo [4,5-f]-quinoline (MeIQx). Only chitosan and pectin showed significant inhibitory effects on HAs, reducing HA levels by 34.5-56.3% and 30.1-56.6%, respectively. In grilled beef patties, the addition of 1.5% chitosan and pectin significantly decreased AGE and HA content by 53.8-67.0% and 46.9-68.1%, respectively. Moreover, it had a limited impact on quality and sensory properties. Further mechanism studies conducted in model systems revealed that chitosan and pectin decreased the formation of key intermediates of AGEs and HAs. These findings suggest that chitosan and pectin are powerful inhibitors against AGE and HA formation with minimal impact on food quality. Therefore, their application in meat preparation and processing could effectively decrease human dietary exposure to HAs and AGEs.
摘要：
Deep eutectic solvent (DES) and hot-water extraction (HWE) methods were utilized to extract polysaccharides from Polygonatum sibiricum, referred to as DPsP and WPsP, respectively. The extracted polysaccharides were purified using the Superdex-200 dextran gel purification system, resulting in three components for each type of polysaccharide. The structures of these components were characterized. The molecular weight analysis revealed that DPsP components had slightly larger molecular weights compared with WPsP, with DPsP-A showing a slightly higher dispersity index and broader molecular weight distribution. The main monosaccharide components of both DPsP and WPsP were mannose and glucose, while DPsP exhibited a slightly greater variety of sugar components compared with WPsP. FTIR analysis demonstrated characteristic polysaccharide absorption peaks in all six PSP components, with a predominance of acidic pyranose sugars. NMR analysis revealed the presence of pyranose sugars, including rhamnose and sugar aldehyde acids, in both DPsP-B and WPsP-A. DPsP-B primarily exhibited β-type glycosidic linkages, while WPsP-A predominantly displayed α-type glycosidic linkages, with a smaller fraction being β-type. These findings indicated differences in monosaccharide composition and structure between PSPs extracted using different methods. Overall, this study provided experimental evidence for future research on the structure-function relationship of PSPs.
通讯机构：
[Han, XX ; Chen, F] H;Hunan Univ, Natl Engn Res Ctr High Efficiency Grinding, Changsha 410082, Peoples R China.;Hunan Univ, State Key Lab Adv Design & Manufacture Vehicle Bod, Changsha 410082, Peoples R China.
摘要：
Light-based 3D bioprinting is now employed widely to fabricate geometrically complex constructs for various biomedical applications. However, the inherent light scattering defect creates significant challenges in patterning dilute hydrogels to form high-fidelity structures with fine-scale features. Herein, we introduce a photoinhibiting approach that can effectively suppress the light scattering effect via a mechanism of simultaneous photoabsorption and free-radical reaction. This biocompatible approach significantly improves the printing resolution (~1.2 - ~2.1 pixels depending on swelling) and shape fidelity (geometric error less than 5%), while minimising the costly trial-and-error procedures. The capability in patterning 3D complex constructs using different hydrogels is demonstrated by manufacturing various scaffolds featuring intricate multi-sized channels and thin-walled networks. Importantly, cellularised gyroid scaffolds (HepG2) are fabricated successfully, exhibiting high cell proliferation and functionality. The strategy established in this study promotes the printability and operability of light-based 3D bioprinting systems, allowing numerous new applications for tissue engineering. ‘Light-based bioprinting is employed in the fabrication of complex constructs but achieving high resolution remains challenging due to light scattering effects. Here, the authors develop a photoinhibiting additive which suppresses light scattering and demonstrate printing of functional scaffolds
通讯机构：
[Xin Zhang; Xiaoxu Sun] S;School of Environment, Key Laboratory of Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, 453007, China<&wdkj&>National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Garo-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, 510650, China<&wdkj&>Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China<&wdkj&>School of Environment, Key Laboratory of Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, 453007, China
通讯机构：
[Qian Zhao; Xingbo Shi] L;Laboratory of Micro & Nano Biosensing Technology in Food Safety, Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要：
Herein, an antioxidant, antibacterial and pH-sensitive multifunctional film is fabricated by incorporating anthocyanins-rich purple sweet potato extract and quercetin-loaded chitosan nanoparticles in agar /sodium alginate polymer matrix for monitoring and maintaining the freshness of shrimp. The film shows distinguishable color changes as pH changes and is highly sensitive to volatile ammonia. Moreover, the digital color information of the film is obtained by smartphone combined with RGB (red/green/blue) analysis. The G/R values are successfully used for the evaluation of shrimp freshness. More importantly, the film exhibits strong UV-blocking, antioxidant, antibacterial properties, and excellent mechanical, water vapor barrier properties, which enables the film to be used as a food packaging film. Notably, shrimp packed with the film can extend shelf life to about 36 h in refrigerated storage. Consequently, the multifunctional film can be applied to intelligent packaging for not only real-time indicating but also high-efficiently preserving the freshness of animal-derived foods.
摘要：
Flavor is an essential element of food quality. Flavor can be improved by adding flavoring substances or via microbial fermentation to impart aroma. Aroma-producing yeasts are a group of microorganisms that can produce aroma compounds, providing a strong aroma to foods and thus playing a great role in the modern fermentation industry. The physiological characteristics of aroma-producing yeast, including alcohol tolerance, acid tolerance, and salt tolerance, are introduced in this article, beginning with their origins and biological properties. The main mechanism of aroma-producing yeast is then analyzed based on its physiological roles in the fermentation process. Functional enzymes such as proteases, lipases, and glycosidase are released by yeast during the fermentation process. Sugars, fats, and proteins in the environment can be degraded by these enzymes via pathways such as glycolysis, methoxylation, the Ehrlich pathway, and esterification, resulting in the production of various aromatic esters (such as ethyl acetate and ethyl caproate), alcohols (such as phenethyl alcohol), and terpenes (such as monoterpenes, sesquiterpenes, and squalene). Furthermore, yeast cells can serve as cell synthesis factories, wherein specific synthesis pathways can be introduced into cells using synthetic biology techniques to achieve high-throughput production. In addition, the applications of aroma yeast in the food, pharmaceutical, and cosmetic industries are summarized, and the future development trends of aroma yeasts are discussed to provide a theoretical basis for their application in the food fermentation industry.
通讯机构：
[Xia Liu] H;Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Long-term use of norfloxacin (NOR) will cause NOR residues in foods and harm human bodies. The determination of NOR residues is important for guaranteeing food safety. In this study, a simple, selective, and label-free colorimetric chemosensor for in situ NOR detection was developed based on Fe3O4 magnetic molecularly imprinted nanoparticles (Fe3O4 MMIP NPs). The Fe3O4 MMIP NPs showed good peroxidase-like catalytic activity to 3,3 ',5,5 '-tetramethylbenzidine (TMB) and selective adsorption ability to NOR. The colorimetric chemosensor was constructed based on the Fe3O4 MMIP NPs-H2O2-TMB reaction system. The absorbance differences were proportional to the concentrations of NOR in the range of 10-300 ng/mL with a limit of detection at 9 ng/mL. The colorimetric chemosensor was successfully applied to detect NOR residue in milk. The recovery range was 78.2-95.81%, with a relative standard deviation of 2.1-9.88%. Together, the proposed colorimetric chemosensor provides a reliable strategy for the detection of NOR residues in foods.
