摘要：
A simple, selective, highly sensitive, and label-free electrochemical immunosensor for atrazine detection was developed by immobilizing gold nanoparticles (GNPs) on the gold electrode surface. The modified gold electrode exhibited good electrochemical activity. The high sensitive detection was achieved by increased the surface area of work electrode resulting in capturing more anti-atrazine monoclonal antibodies. The whole immunosensor fabrication process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with the use of ferricyanide as an electrochemical redox indicator, respectively. The signal was triggered based on the interaction between anti-atrazine monoclonal antibody and atrazine via differential pulse voltammetry (DPV). Under the optimal conditions, a limit of detection for atrazine as low as 0.016 ng/mL was obtained in buffer, and the linear working range was between 0.05 ng/mL and 0.5 ng/mL. The recovery of 95.5-119.8% was obtained in maize. The proposed immunosensor has acceptable stability and reproducibility, thus could be applied to other pesticides analysis of crop samples in practice. (C) 2013 Elsevier B.V. All rights reserved.
作者机构：
[Li, Ying; Liu, Xia; Lin, Zhao] College of Food Science and Technology, Hunan Agricultural University, Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha 410128, China
关键词：
Fuzhuan brick-tea;Fungal community;Denaturing gradient gel electrophoresis;Eurotium cristatum;Debaryomyces hansenii;Anthraquinone
摘要：
Chinese Fuzhuan brick-tea is a unique microbial fermented tea characterized by a period of fungal growth during its manufacturing process. The aim of the present study was to characterize, both physicochemically and microbiologically, traditional industrial production processes of Fuzhuan brick-tea. Fermenting tea samples were collected from the largest manufacturer. Physicochemical analyses showed that the low water content in the tea substrates provided optimal growth conditions for xerophilic fungi. The fungal communities existing in tea materials, fermenting tea, and stored teas were monitored by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) targeting the D1 region of the 265 rRNA genes, followed by sequencing of the amplicons. Results revealed that the microorganisms were from, or closely related to, the genera Eurotium, Debaryomyces, Aspergillus, Verticillium, Pichia, Pestalotiopsis, Rhizomucor and Beauveria. This is the first report of Debaryomyces participating in the processing of Fuzhuan brick-tea. We concluded that the dominant genera Eurotium, Debaryomyces and Aspergillus are beneficial fungi associated with the fermentation of Fuzhuan brick-tea. The genus Beauveria was present in the stored Fuzhuan brick-tea, which may help protect tea products from insect spoilage. The remaining four genera were of minor importance in the manufacturing of Fuzhuan brick-tea. The predominant Eurotium species, a strain named Eurotium sp. FZ, was phenotypically and genotypically identified as Eurotium cristatum. High performance thin layer chromatography analysis of anthraquinones showed that emodin existed in all the dark tea samples, but physcion was only detectable in the tea fermented by E. cristatum. The PCR-DGGE approach was an effective and convenient means for profiling the fungal communities in Fuzhuan brick-tea. These results may help promote the use of microbial consortia as starter cultures to stabilize and improve the quality of Fuzhuan brick-tea products. (C) 2011 Elsevier B.V. All rights reserved.
作者：
Zhenhua Qiu;Pengjun Shi;Huiying Luo;Yingguo Bai;Tiezheng Yuan;Peilong Yang;Suchun Liu;Bin Yao
期刊：
Enzyme and Microbial Technology,2010年46(6):506-512 ISSN：0141-0229
通讯作者：
Yao, B.(yaobin@caas-bio.net.cn)
作者机构：
[Huiying Luo; Peilong Yang; Yingguo Bai; Zhenhua Qiu; Tiezheng Yuan; Pengjun Shi] Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, PR China;[Bin Yao] Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, PR China. Electronic address: yaobin@caas-bio.net.cn;[Zhenhua Qiu; Suchun Liu] Department of Nutrition and Food Hygiene, Hunan Agricultural University, Changsha, Hunan 410128, PR China
通讯机构：
[Yao, Bin] Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, PR China. Electronic address:
作者机构：
[Qin, Si; Hou, De-Xing] Core Research Program 1515, Key Laboratory for Food Science and Biotechnology of Hunan Province, College of Food Science and Technology, Hunan Agricultural University, Changsha, China;[Qin, Si; Hou, De-Xing] Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, China;[Hou, De-Xing] The United Graduate School of Agricultural Sciences, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan
通讯机构：
[Hou, De-Xing] Hunan Agr Univ, Coll Food Sci & Technol, Core Res Program 1515, Key Lab Food Sci & Biotechnol Hunan Prov, Changsha, Hunan, Peoples R China.;[Hou, De-Xing] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha, Hunan, Peoples R China.;[Hou, De-Xing] Kagoshima Univ, United Grad Sch Agr Sci, Fac Agr, Kagoshima, Japan.
摘要：
Keap1/Nrf2 system plays a critical role on cellular protection by regulating many antioxidant and detoxification enzyme genes through the antioxidant response element (ARE). Thus, it must work constantly to prevent the accumulation of reactive oxygen species (ROS) because excess ROS are associated with many diseases such as cancer, cardiovascular complications, inflammation, and neurodegeneration. Dietary phytochemicals widely distributing in fruits and vegetables have been considered to possess cancer chemopreventive potential through the induction of Keap1/Nrf2 system‐mediated antioxidant and detoxification enzymes in a variety of manners. The data are extensive and are not well classified on the molecular mechanisms. In this review, we first briefly introduce the current knowledge on Keap1/Nrf2 system regulation including Keap1‐dependent and Keap1‐independent cascades, and epigenetic pathway. Then, we summarize the molecular targets of Keap1/Nrf2 system by dietary phytochemicals, and finally review the crosstalk between Keap1/Nrf2 system and other cellular signaling pathways to regulate diverse chronic diseases by dietary phytochemicals. These comprehensive data will help us to understand the potential effects of dietary phytochemicals on the prevention of chronic diseases and maintenance of human health.
期刊：
MITOCHONDRIAL DNA PART B-RESOURCES,2019年4(1):139-140 ISSN：2380-2359
通讯作者：
Jiang, Gui-Tao
作者机构：
[Peng, Si-Min] College of Food Science and Technology, Hunan Agricultural University, Changsha, China;[Lin, Qian; Dai, Qiu-Zhong; Jiang, Gui-Tao] Research Department of Animal Nutrition and Poultry, Hunan Institute of Animal and Veterinary Science, Changsha, China;[Lin, Qian; Dai, Qiu-Zhong] ;[Lin, Qian; Dai, Qiu-Zhong] Institute of Bast Fiber Crops Chinese Academy of Agricultural Sciences, Changsha, China;[He, Xi] College of Animal Science and Technology, Hunan Agricultural University, Changsha, China
通讯机构：
[Jiang, Gui-Tao] Hunan Inst Anim & Vet Sci, Res Dept Anim Nutr & Poultry, Changsha 410131, Hunan, Peoples R China.;Research Department of Animal Nutrition and Poultry, Hunan Institute of Animal and Veterinary Science, Changsha, China
摘要：
Abstract Zhijiang duck (ZJ) is one of the famous indigenous breed of Hunan province in China. The complete mitochondrial genome sequence plays an important role in the accurate determination of phylogenetic relationships among metazoans. It is the first time that the complete mitochondrial genome sequence of the ZJ was reported. The total length of the mtDNA is 16,604 bp. It contains the typical structure, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and 1 non-coding control region (D-loop region). The overall composition of the mtDNA was estimated to be 29.22% for A, 22.20% for T, 32.82% for C, and 15.77% for G. Phylogenetic analyses using N-J computational algorithms showed that the analyzed 20 Anseriform species are divided into four major clades: Anatinae, Anserinae, Dendrocygninae, and Anseranatidae. In addition, our work confirmed that ZJ and Anas poecilorhyncha have a close genetic relationship with fellow tribal members Linwu duck and Youxian duck. Meanwhile, we also found that ZJ and A. poecilorhyncha have highly similar genetic relationship. This work will provides an important data set for the study in the genetic mechanism of duck in Hunan province.
摘要：
Catechin exhibits antimicrobial activity against various microorganisms, such as EHEC O157:H7. This study reports the bactericidal effect of catechin on EHEC O157:H7 in simulated human gastrointestinal environment and the underlying antibacterial mechanism. Bacteriostasis test results showed that the minimum bactericidal concentration of catechin for EHEC O157:H7 was 5g/L. The bactericidal effect of catechin in the gastrointestinal juice became more significant with increased culture time, and catechin exhibited a synergistic effect with bile salt in inhibiting EHEC O157:H7. Changes in the profile of protein expression in EHEC O157:H7 in response to catechin intervention were investigated. Two-dimensional electrophoresis identified 34 proteins with significantly altered expression. A total of 2 and 12 proteins were upregulated and downregulated, respectively. However, 20 proteins disappeared. No new protein was expressed compared with the control. Hence, catechin intervention resulted in diverse changes in the expression of proteins associated with cell structure and genetic information processing. Catechin could cause the disappearance of certain proteins or the destruction of certain peptides. These processes lead to the inhibition of EHEC O157: H7 cells.
摘要：
A colorimetric assay is described for acrylamide (AA). It is based on color changes induced by an increase in the distance between gold nanoparticles (AuNPs) that is caused by AA copolymerization. First, AuNPs were modified with a thiolated propylene amide poly(ethylene glycol) that also contains the AA functionality. The carbon-carbon double bonds on the modified AuNPs can be polymerized under the catalysis of a photoinitiator and under UV irradiation. This results in the aggregation of the AuNPs and in a color change from red to gray. In the presence of AA, the distance between the AuNPs increases due to copolymerization with AA, and the solution of AuNPs preserves its original red color. Under optimized conditions, the absorption ratio (A525/A740) of the solution increases linearly in the 1 nM to 10 μM free AA concentration range, with a 0.2 nM limit of detection. Hence, the method meets the need for rapid monitoring of trace AA in food. The method has a relative error (RSD) that is lower compared to the accepted HPLC method. Schematic of a novel colorimetric strategy for acrylamide (AA) detection based on the increase of distance between gold nanoparticles (AuNPs) caused by AA participated polymerization.
作者机构：
[Tata, Anuradha; Neto, Catherine] C. Neto, Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, Dartmouth, MA, USA;[Wu, Xian; Xiao, Hang; Wang, Qi; Song, Mingyue; Cai, Xiaokun; Han, Yanhui] X. Wu, M. Song, X. Cai, Y. Han, Q. Wang, H. Xiao, Department of Food Science, University of Massachusetts, Amherst, MA, USA;[Song, Mingyue] M. Song, College of Food Science, South China Agricultural University, Guangzhou, P. R. China;[Tang, Zhonghai] Z. Tang, College of Food Science and Technology, Hunan Agricultural University, Changsha, P. R. China
通讯机构：
[Xiao, Hang] Univ Massachusetts, Dept Food Sci, Amherst, MA 01003 USA.
摘要：
SCOPE: There are growing interests in using whole food-based approach to prevent chronic diseases due to potential synergistic interactions among different bioactive components within the whole foods. North American cranberry (Vaccinium macrocarpon), a polyphenol-rich fruit, has been shown to exert multiple beneficial health effects. METHODS AND RESULTS: For the first time, we determined the protective effects of whole cranberry powder (WCP) against colitis-associated mouse colon tumorigenesis induced by azoxymethane (AOM) and dextran sulfate sodium (DSS). The results showed that dietary administration of WCP (1.5%, w/w in the diet) significantly suppressed colon tumorigenesis as indicated by the reduced tumor incidence, multiplicity, burden and average tumor size in WCP-fed mice compared to the positive control mice. Both gene and protein expression levels of pro-inflammatory cytokines IL-1beta, IL-6 and TNF-alpha were markedly attenuated by WCP treatment in the colon of AOM/DSS-treated mice. Moreover, WCP profoundly modulated multiple signaling pathways/proteins related to inflammation, cell proliferation, apoptosis, angiogenesis and metastasis in the colon, which was closely associated with the inhibitory effects of WCP on colon tumorigenesis. CONCLUSION: Overall, our results demonstrated chemopreventive effects of WCP on colon tumorigenesis in mice, providing a scientific basis for using the whole cranberry as a functional food to promote colon health in humans. This article is protected by copyright. All rights reserved.
作者机构：
[Zhang, Ke; Tong, Zhongyi; Huang, Feifei; Tan, Hong; Li, Daiqiang; Guo, Tianyao] Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China;[Wu, Yanyang] Food Science and Technology College, Hunan Agricultural University, Changsha, Hunan 410128, P.R. China
通讯机构：
[Tong, Zhongyi] Cent S Univ, Xiangya Hosp 2, Dept Pathol, 139 Renmin Rd, Changsha 410011, Hunan, Peoples R China.
关键词：
phosphorylation of nucleolin;nucleolin;doxorubicin;proliferation;migration;non-small cell lung cancer
摘要：
Non-small cell lung cancer (NSCLC) is one of the mostly deadly malignancies in the world. Nucleolin is a multifunctional protein that mainly regulates ribosome biogenesis but also has other functions including modulating the transcription of mRNAs and repressing RNA polymerase II. Nucleolin is overexpressed in various cancer cells, including NSCLC cells. It can confer resistance to apoptosis and promote cell migration and blood vessel formation by directly taking part in various tumor signal transduction pathways. The activities of nucleolin are regulated mainly by intracellular localization and post-translational modifications, including phosphorylation, glycosylation, methylation, and ADP-ribosylation. Phosphorylation of nucleolin (P-nucleolin) in NSCLC cells is still not well characterized. In the present study, the levels of nucleolin and P-nucleolin were examined in lung tissue and cells and it was demonstrated that levels of the two forms of nucleolin were significantly increased in NSCLC compared with non-cancerous tissues and cells. In addition, it was demonstrated that high expression levels of nucleolin and P-nucleolin were significantly associated with poor overall survival of NSCLC patients. Doxorubicin (DOX) is a type of anthracycline that has been used in the treatment of various types of cancer, including NSCLC. Upregulation of nucleolin through exogenous expression of nucleolin promoted A549 cell proliferation and migration, while downregulation of nucleolin through expression of small interfering RNA-nucleolin attenuated A549 cell proliferation and migration. Following stimulation with DOX, A549 cell proliferation and migration decreased and the expression of P-nucleolin also decreased. In order to investigate whether P-nucleolin is indispensable to the proliferation and migration of NSCLC cells, a plasmid encoding mutant nucleolin, in which the phosphorylation site at threonine-76 was mutated to alanine, was constructed. Compared with the A549 cells transfected with wild-type nucleolin, P-nucleolin expression and cell proliferation and migration were significantly decreased in A549 cells transfected with mutant nucleolin. These results indicate that targeting P-nucleolin may be a promising strategy for treating NSCLC patients.
作者机构：
[Wu, Xian; Rakariyatham, Kanyasiri; Wang, Qi; Han, Yanhui] Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA. hangxiao@foodsci.umass.edu;[Xiao, Hang] College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China and Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA. hangxiao@foodsci.umass.edu;[Tang, Zhonghai] College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Xiao, Hang] College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China and Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.;[Rakariyatham, Kanyasiri] Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA.
摘要：
Luteolin and sulforaphane are well-known food bioactives with anti-inflammatory properties. Herein, we determined their combinational effects in inhibiting inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both luteolin and sulforaphane showed dose-dependent inhibition on LPS-induced production of nitric oxide (NO) in the macrophages. The combined treatments led to a stronger inhibition on NO production compared to the singular treatments. Isobologram analysis confirmed that the combined treatments produced a synergy. Western blotting and ELISA showed that the combined treatment reduced the expression levels of pro-inflammatory proteins involving NF-kappaB pathway, and STAT3 activation, which regulated expression of other inflammatory proteins such as iNOS, COX-2, IL-6, and IL-1beta. Moreover, the combination treatments reduced reactive oxygen species in cells and increased the expression of Nrf2 and HO-1, which are cellular antioxidant proteins. In conclusion, our findings support the notion that certain bioactive food components may act synergistically to produce enhanced health effects such as anti-inflammation.
关键词：
Nisin-resistance;Cell wall;Membrane phospholipid;Two-component system
摘要：
Nisin is the most prominent lantibiotic and is used as a food preservative due to its high potency against certain Gram-positive bacteria. However, the effectiveness of nisin is often affected by environmental factors such as pH, temperature, food composition, structure, as well as food microbiota. The development of nisin resistance has been seen among various Gram-positive bacteria. The mechanisms under the acquisition of nisin resistance are complicated and may differ among strains. This paper presents a brief review of possible mechanisms of the development of resistance to nisin among Gram-positive bacteria.
通讯机构：
[Yin, Huaqun] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
摘要：
Response of biological communities to environmental stresses is a critical issue in ecology, but how microbial communities shift across heavy metal gradients remain unclear. To explore the microbial response to heavy metal contamination (e.g., Cr, Mn, Zn), the composition, structure and functional potential of sedimentary microbial community were investigated by sequencing of 16S rRNA gene amplicons and a functional gene microarray. Analysis of 16S rRNA sequences revealed that the composition and structure of sedimentary microbial communities changed significantly across a gradient of heavy metal contamination, and the relative abundances were higher for Firmicutes, Chloroflexi and Crenarchaeota, but lower for Proteobacteria and Actinobacteria in highly contaminated samples. Also, molecular ecological network analysis of sequencing data indicated that their possible interactions might be enhanced in highly contaminated communities. Correspondently, key functional genes involved in metal homeostasis (e.g., chrR, metC, merB), carbon metabolism, and organic remediation showed a higher abundance in highly contaminated samples, indicating that bacterial communities in contaminated areas may modulate their energy consumption and organic remediation ability. This study indicated that the sedimentary indigenous microbial community may shift the composition and structure as well as function priority and interaction network to increase their adaptability and/or resistance to environmental contamination.
关键词：
Fish gelatin;Acid pretreatment;Protein yield;Molecular weight distribution;Viscosity;Film
摘要：
Tilapia production is growing worldwide and to better utilize wastes from the processing industry, one important application is production of high quality fish gelatin to meet the needs of markets that are not amenable to beef or porcine gelatin. The extraction process from tilapia skin gelatin was optimized through the use of a combination of alkali (0.3 M NaOH) with different types and concentrations of acids before thermal hydrolysis. The effects of acid pretreatments on the protein yields and the physicochemical properties of tilapia gelatin were investigated. Acid concentrations (0.01-0.20 M) influenced gelatin protein recovery: 10.52%-22.40% for citric acid, 1.92%-21.55% for acetic acid, and 4.47%-24.35% for HCl. It was possible to increase gelatin yield for each of the tested acids by adjusting the acid concentration. Gelatin viscosity and the molecular weight distribution of gelatin proteins were related to the acid concentration used. Gelatin prepared using too low a concentration (e.g. 0.01 M acetic acid or HCl) or too high a concentration (e.g. >0.05 M HCl or citric acid) yielded an extract with a smaller ratio of large molecule components, such as beta-chains, and exhibited lower viscosity. The film forming properties of gelatins extracted from three acid-optimized pretreatments showed no significant difference in transparency, tensile strength and elongation at break; though the gelatin film made from 0.03 M citric acid pretreated gelatin had somewhat better water barrier property than those made with HCl or acetic acid. (C) 2013 Elsevier Ltd. All rights reserved.
期刊：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2015年16(7):14511-14525 ISSN：1422-0067
通讯作者：
Jiang, BM
作者机构：
[Xiao, Xianzhong] Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410000, Hunan, China. xiaoxianzhong@csu.edu.cn;[Li, Yuanbin; Liu, Yanjuan] Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410000, Hunan, China. LYB820812@126.com;[Tong, Zhongyi] Department of Pathology, the Second Xiangya Hospital, Central South University, Changsha 410000, Hunan, China. tongzhongyi2008@163.com;[Tong, Zhongyi] Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410000, Hunan, China. tongzhongyi2008@163.com;[Lv, Qinglan] Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410000, Hunan, China. lvqinglan2014@163.com
通讯机构：
[Jiang, Bimei] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410000, Hunan, Peoples R China.
摘要：
Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of antineoplastic activities. However, it causes cardiac cytotoxicity, and this limits its clinical applications. MicroRNA-21 (miR-21) plays a vital role in regulating cell proliferation and apoptosis. While miR-21 is preferentially expressed in adult cardiomyocytes and involved in cardiac development and heart disease, little is known regarding its biological functions in responding to DOX-induced cardiac cytotoxicity. In this study, the effects of DOX on mouse cardiac function and the expression of miR-21 were examined in both mouse heart tissues and rat H9C2 cardiomyocytes. The results showed that the cardiac functions were more aggravated in chronic DOX injury mice compared with acute DOX-injury mice; DOX treatment significantly increased miR-21 expression in both mouse heart tissue and H9C2 cells. Over-expression of miR-21 attenuated DOX-induced apoptosis in cardiamyocytes whereas knocking down its expression increased DOX-induced apoptosis. These gain- and loss- of function experiments showed that B cell translocation gene 2 (BTG2) was a target of miR-21. The expression of BTG2 was significantly decreased both in myocardium and H9C2 cells treated with DOX. The present study has revealed that miR-21 protects mouse myocardium and H9C2 cells against DOX-induced cardiotoxicity probably by targeting BTG2.
作者：
Hua-xi Xiao 肖华西;Qin-lu Lin 林亲录;Xin-jian Xia 夏新剑;Li-hui Li 李丽辉;Li-zhong Lin 林利忠;Wei-guo Wu 吴卫国
期刊：
Journal of Central South University of Technology,2008年15(S1):500-505 ISSN：1005-9784
通讯作者：
Lin, Q.-L.(lql0403@yahoo.com.cn)
作者机构：
[Hua-xi Xiao 肖华西; Qin-lu Lin 林亲录; Li-hui Li 李丽辉; Li-zhong Lin 林利忠] Faculty of Food Science and Engineering, Center South University of Forestry and Technology, Changsha 410004, China;[Qin-lu Lin 林亲录; Xin-jian Xia 夏新剑; Wei-guo Wu 吴卫国] School of Food and Science, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Lin Qin-lu] Ctr S Univ Forestry & Technol, Fac Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
摘要：
Based on the sweet potato starch, cationic starch, acetic starch and cationic-acetic compoundedly modified starch were made through chemical denaturalization. The above three kinds of static rheological parameter and dynamic rheological parameter were measured, respectively. The experimental result reveals that the thermal stability of starchy viscosity increases after chemical denaturalization. Under the condition of identical shearing rate, the shear stress of cationic-acetic ester compoundedly modified sweet potato starch paste is the largest among these kinds of sweet potato starch. This attributes to a phenomenon of shearing thinning. Furthermore, raw sweet potato starch has a larger gel intensity than that of modified starch.
摘要：
This work compares the polyphenolics composition, antioxidant activity and contents of selected nutrients in the leaves from seven cultivars of Moringa oleifera ('Tumu', 'Sunyaw', 'Kumasi', 'Techiman', 'China', 'Pakistan Black', and 'Pakistan White') representing the variety in Pakistan. Both the non-enzymatic (DPPH free radical and linoleic acid peroxidation inhibition assays) and enzymatic (superoxide dismutase, peroxidase, and catalase activities) antioxidant capacity was appraised. Regarding,the nutritional value, in terms of protein and minerals contents, minor differences were recorded among cultivars. HPLC-PDA-ESI-MSn analysis of the hydro-methanolic extracts of Moringa leaves revealed a wide range of phenolics, highlighting their content in kaempferol derivatives, caffeoylquinic acid, and feruloylquinic acid. The antioxidant capacity of the tested leaves was correlated with the individual phenolics composition in order to identify which compounds were responsible for this beneficial power. The varying (p < 0.05) concentration of phenolics together with the antioxidant capacity of the tested leaves established 'Pakistan Black' and 'Techiman' as the most nutritive cultivar among others to be grown in the local area. Moreover, these data support the relevance of genetic variability of Moringa for determining the aptitude as a source of beneficial phenolics and nutrients, allowing to identify the optima cultivars to be grown in southern Asia. (c) 2015 Elsevier B.V. All rights reserved.
期刊：
Biosensors and Bioelectronics,2014年59:328-334 ISSN：0956-5663
通讯作者：
Liu, X.(liuxiaspr@gmail.com)
作者机构：
[Li, Lei; Liu, You-Qian; Yang, Yang; Li, Wen-Jin; Liu, Xia; Mao, Lu-Gang; Shi, Xing-Bo] College of Food Science and Technology, Hunan Agricultural University, Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha 410128, China;[Liu, Xia] State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China
