通讯机构：
[Wang, RR ; Ding, SH ] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;Hunan Univ, Coll Biol, Changsha 410125, Peoples R China.
关键词：
Starch film;Janus structure;Rapid self-assembly;Fresh-cut fruits and vegetables preservation
摘要：
Starch-based films are promising biodegradable materials for food packaging but are limited by hydrophilicity and poor functional properties. Herein, this study proposed a citral nanoemulsion (NEs)-mediated interfacial engineering strategy to construct a self-assembled Janus starch film (NE-S/M) with dual hydrophobic-active functionality for fresh-cut fruits and vegetables preservation. Specifically, starch extracted from waste rice was composited with citral NEs to form bioactive films (NE-S), which were further rapidly self-assembled with myristic acid (MA) to create a Janus hydrophobic structure. Structural characterization and molecular dynamics simulation revealed that citral NEs acted as molecular bridges to enhance the MA adsorption energy barrier, enabling MA to self-assemble into a dense hydrophobic layer via free deposition. Especially, the 10 %NE-S/M Janus film exhibited exceptional hydrophobicity (water contact angle: 118.22°), UV-blocking (0 % transmittance at 200–400 nm), mechanical robustness (tensile strength enhanced by 180.75 %), and barrier properties (water vapor permeability reduced by 62.39 %). Furthermore, the Janus film synergized the outstanding antioxidant and antimicrobial properties of citral. Compared to starch film, the 10 %NE-S/M Janus film exhibited a three-fold increase in free radical scavenging capacity and notable inhibition on Staphylococcus aureus , Escherichia coli , and Botrytis cinerea with relative inhibition rates of 98.63 %, 91.56 %, and 42.53 %, respectively. Preservation tests demonstrated that 10 %NE-S/M film significantly reduced weight loss, suppressed microbial proliferation, and delayed quality deterioration in fresh-cut kiwifruit and chili peppers throughout storage. Crucially, this rapid self-assembly strategy successfully achieved hydrophobicity and bioactive properties in starch-based film and offered a sustainable solution for active food packaging with multifunctional performance.
Starch-based films are promising biodegradable materials for food packaging but are limited by hydrophilicity and poor functional properties. Herein, this study proposed a citral nanoemulsion (NEs)-mediated interfacial engineering strategy to construct a self-assembled Janus starch film (NE-S/M) with dual hydrophobic-active functionality for fresh-cut fruits and vegetables preservation. Specifically, starch extracted from waste rice was composited with citral NEs to form bioactive films (NE-S), which were further rapidly self-assembled with myristic acid (MA) to create a Janus hydrophobic structure. Structural characterization and molecular dynamics simulation revealed that citral NEs acted as molecular bridges to enhance the MA adsorption energy barrier, enabling MA to self-assemble into a dense hydrophobic layer via free deposition. Especially, the 10 %NE-S/M Janus film exhibited exceptional hydrophobicity (water contact angle: 118.22°), UV-blocking (0 % transmittance at 200–400 nm), mechanical robustness (tensile strength enhanced by 180.75 %), and barrier properties (water vapor permeability reduced by 62.39 %). Furthermore, the Janus film synergized the outstanding antioxidant and antimicrobial properties of citral. Compared to starch film, the 10 %NE-S/M Janus film exhibited a three-fold increase in free radical scavenging capacity and notable inhibition on Staphylococcus aureus , Escherichia coli , and Botrytis cinerea with relative inhibition rates of 98.63 %, 91.56 %, and 42.53 %, respectively. Preservation tests demonstrated that 10 %NE-S/M film significantly reduced weight loss, suppressed microbial proliferation, and delayed quality deterioration in fresh-cut kiwifruit and chili peppers throughout storage. Crucially, this rapid self-assembly strategy successfully achieved hydrophobicity and bioactive properties in starch-based film and offered a sustainable solution for active food packaging with multifunctional performance.
期刊：
Journal of Colloid and Interface Science,2026年702(Pt 1):138837 ISSN：0021-9797
通讯作者：
Gao, Zhongfeng
作者机构：
[Li, Xinyuan; Sui, Xin; Liu, Fanglan; Li, Yingqi; Li, Na; Mai, Xi; Wang, Cheng; Long, Qinghong] School of Pharmacy, Jiangxi Medical College, Nanchang University, Jiangxi Province Key Laboratory of New Drug Evaluation and Transformation, Nanchang 330031, PR China;[Li, Yanlei] Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China;[Liu, Shigang] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, PR China;[Gao, Zhongfeng] Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.. Electronic address: chm_gaozf@ujn.edu.cn;[Xia, Fan] Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, PR China
通讯机构：
[Gao, Zhongfeng] K;Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.. Electronic address:
摘要：
Employing antioxidant nanozymes to eliminate reactive oxygen species (ROS) is a promising strategy for alleviating oxidative stress. However, most current nanozymes struggle to balance catalytic efficacy with biosafety, limiting their clinical applicability. In this study, we introduce a novel platform: DNA nanoribbon-templated copper nanoclusters (DNR/Cu NCs), which harness dual antioxidative mechanisms (direct ROS scavenging and activation of nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway) to synergistically mitigate oxidative stress. Unlike conventional nanozymes that rely on a single mechanism, DNR/Cu NCs exhibit combined superoxide dismutase (SOD)/catalase (CAT)/glutathione peroxidase (GPx), thereby enhancing overall ROS elimination. The biocompatible DNR scaffold facilitates the formation of ultrasmall Cu NCs with high catalytic activity and promotes NRF2 nuclear translocation to transcriptionally upregulate HO-1, amplifying endogenous antioxidant defenses. In both hepatocyte and zebrafish models of oxidative injury, the DNR/Cu NCs effectively suppressed ROS accumulation, suppressed apoptosis, and restored redox balance while mitigating tissue damage in vivo. This study highlights a paradigm-shifting approach in nanozyme design, offering a promising therapeutic avenue for oxidative stress-related diseases.
Employing antioxidant nanozymes to eliminate reactive oxygen species (ROS) is a promising strategy for alleviating oxidative stress. However, most current nanozymes struggle to balance catalytic efficacy with biosafety, limiting their clinical applicability. In this study, we introduce a novel platform: DNA nanoribbon-templated copper nanoclusters (DNR/Cu NCs), which harness dual antioxidative mechanisms (direct ROS scavenging and activation of nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway) to synergistically mitigate oxidative stress. Unlike conventional nanozymes that rely on a single mechanism, DNR/Cu NCs exhibit combined superoxide dismutase (SOD)/catalase (CAT)/glutathione peroxidase (GPx), thereby enhancing overall ROS elimination. The biocompatible DNR scaffold facilitates the formation of ultrasmall Cu NCs with high catalytic activity and promotes NRF2 nuclear translocation to transcriptionally upregulate HO-1, amplifying endogenous antioxidant defenses. In both hepatocyte and zebrafish models of oxidative injury, the DNR/Cu NCs effectively suppressed ROS accumulation, suppressed apoptosis, and restored redox balance while mitigating tissue damage in vivo. This study highlights a paradigm-shifting approach in nanozyme design, offering a promising therapeutic avenue for oxidative stress-related diseases.
期刊：
Sensors and Actuators B-Chemical,2026年448:138928 ISSN：0925-4005
通讯作者：
Zhihui Wu<&wdkj&>Shi Gang Liu
作者机构：
[Zhihui Wu; Xiangzhi Li; Yucong Ma; Fangze Qin; Shi Gang Liu] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China;Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China;[Haoyang He; Hongbin Pu] School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China<&wdkj&>Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, China<&wdkj&>Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China
通讯机构：
[Zhihui Wu; Shi Gang Liu] H;Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
摘要：
Aflatoxin B1 (AFB1) contamination in foods threatens human health worldwide, and accurate and rapid detection of AFB1 is essential. Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-mode aptasensor is constructed for sensitive detection of AFB1 based on ZnFe 2 O 4 magnetic nanoparticle@gold nanoparticles-silver shell nanostructures (MNP@AuNPs-Ag) and fluorescent N-doped carbon dots (N-CDs). Specifically, Raman beacon molecule cyanine 3-modified aptamer (Apt-Cy3) is coupled on the MNP@AuNPs-Ag surface, and complementary DNA (cDNA) is modified on the N-CDs surface (N-CDs-cDNA). In the absence of AFB1, thanks to the DNA hybridization, low fluorescence for the supernatant and low SERS for the precipitate appear after magnetic separation. However, in the presence of AFB1, most of N-CDs-cDNA are free as the aptamer preferentially binds to AFB1, resulting in a strong fluorescent signal in the supernatant, while the change in the secondary structure of the aptamer shortens the spatial distance between Cy3 and MNP@AuNPs-Ag, producing a strong SERS signal. Based on the principle, the dual-mode aptasensor is developed and quantifies AFB1 using SERS and fluorescence signals with the low detection limits of 0.88 and 9.74 pg/mL, respectively. What's more, the dual-mode aptasensor is applied to the detection of AFB1 in real food samples successfully. This work exploits magnetic plasmonic core-shell nanostructures and fluorescent N-CDs to obtain fluorescence and SERS signals from the supernatant and the precipitate respectively after magnetic separation. The strategy not only provides a robust method for detection of AFB1 but also offers new insights for the design of dual-mode aptasensor.
Aflatoxin B1 (AFB1) contamination in foods threatens human health worldwide, and accurate and rapid detection of AFB1 is essential. Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-mode aptasensor is constructed for sensitive detection of AFB1 based on ZnFe 2 O 4 magnetic nanoparticle@gold nanoparticles-silver shell nanostructures (MNP@AuNPs-Ag) and fluorescent N-doped carbon dots (N-CDs). Specifically, Raman beacon molecule cyanine 3-modified aptamer (Apt-Cy3) is coupled on the MNP@AuNPs-Ag surface, and complementary DNA (cDNA) is modified on the N-CDs surface (N-CDs-cDNA). In the absence of AFB1, thanks to the DNA hybridization, low fluorescence for the supernatant and low SERS for the precipitate appear after magnetic separation. However, in the presence of AFB1, most of N-CDs-cDNA are free as the aptamer preferentially binds to AFB1, resulting in a strong fluorescent signal in the supernatant, while the change in the secondary structure of the aptamer shortens the spatial distance between Cy3 and MNP@AuNPs-Ag, producing a strong SERS signal. Based on the principle, the dual-mode aptasensor is developed and quantifies AFB1 using SERS and fluorescence signals with the low detection limits of 0.88 and 9.74 pg/mL, respectively. What's more, the dual-mode aptasensor is applied to the detection of AFB1 in real food samples successfully. This work exploits magnetic plasmonic core-shell nanostructures and fluorescent N-CDs to obtain fluorescence and SERS signals from the supernatant and the precipitate respectively after magnetic separation. The strategy not only provides a robust method for detection of AFB1 but also offers new insights for the design of dual-mode aptasensor.
作者：
Ding, Bonan;Liu, Jiaqi;Kasay, Igor Lumbeto Tenda;Konsue, Nattaya;Lin, Xuan;...
期刊：
Journal of Ethnopharmacology,2026年354:120519 ISSN：0378-8741
通讯作者：
Lv, Chenghao;Xia, Hongjuan;Qin, Si
作者机构：
[Kasay, Igor Lumbeto Tenda; Liu, Jiaqi; Qin, Si; Yan, Fangfang; Ding, Bonan] Lab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[Konsue, Nattaya] Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai, 57100, Thailand;[Lin, Xuan] Department of Endocrinology, CR & WISCO General Hospital, Wuhan University of Science and Technology, Wuhan, 430080, China;[Wang, Dongsheng] Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China;[Lv, Chenghao] Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address: lvchenghao@xiangya.com.cn
通讯机构：
[Xia, Hongjuan] L;[Lv, Chenghao; Qin, Si] I;Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address:;Lab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China. Electronic address:;Institute of Integrative Medicine, Hunan Provincial Key Laboratory of Liver Visceral Manifestation in Traditional Chinese Medicine, Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address:
摘要：
Ethnopharmacological relevance Corus officinalis Siebold & Zucc belongs to the genus Cornus in the Cornaceae family, and was first recorded in the “Shennong Herbal Classic”, now has been included in “according to the tradition of both food and Chinese herbal medicines”, consist of kidney and liver tonifying, antioxidant substances including cycloid glycosides, flavonoids, polyphenols, organic acids, etc.
Corus officinalis Siebold & Zucc belongs to the genus Cornus in the Cornaceae family, and was first recorded in the “Shennong Herbal Classic”, now has been included in “according to the tradition of both food and Chinese herbal medicines”, consist of kidney and liver tonifying, antioxidant substances including cycloid glycosides, flavonoids, polyphenols, organic acids, etc.
Aim of the study This study was aimed at discovering the mechanism underlying the anti-hyperemia effect of Cor in rats, particularly its protective effect against liver and kidney dysfunction caused by HUA.
This study was aimed at discovering the mechanism underlying the anti-hyperemia effect of Cor in rats, particularly its protective effect against liver and kidney dysfunction caused by HUA.
Materials and methods In this study, the effect of Cor extract against HUA was verified in rats, subsequently, network pharmacology combined with non-targeted metabolomic were performed to investigate its composition characteristics, and further multi-omics studies and molecular validation were performed to reveal molecular mechanism both in vivo and in vitro .
In this study, the effect of Cor extract against HUA was verified in rats, subsequently, network pharmacology combined with non-targeted metabolomic were performed to investigate its composition characteristics, and further multi-omics studies and molecular validation were performed to reveal molecular mechanism both in vivo and in vitro .
Results The results demonstrated that cornuside, hydroxygenkwanin and tetrahydroalstonine were the main bioactive compounds in Cor extract, which protected intestinal metabolism disorder by increasing relative abundances of Bacteroides, Lactobacillus Roseburia and Akkermansia , alleviated liver oxidative damage by activation of the Nrf2/HO-1(NQO1) antioxidant pathway, and reduced liver UA synthesis by inhibiting the expression of UA synthesis protein XOD in rat model. In addition, tetrahydroalstonine alleviated inflammation via inhibiting PI3K/Akt/NF-κB signaling pathway, with cornuside and hydroxygenkwanin enhanced renal tubule UA transport capacity by regulating the translations of XDH and HRP genes, all of which protected HUA-rat kidney from inflammatory infiltration damage, and reduced serum urea nitrogen (BUN), creatinine (CRE) and UA levels.
The results demonstrated that cornuside, hydroxygenkwanin and tetrahydroalstonine were the main bioactive compounds in Cor extract, which protected intestinal metabolism disorder by increasing relative abundances of Bacteroides, Lactobacillus Roseburia and Akkermansia , alleviated liver oxidative damage by activation of the Nrf2/HO-1(NQO1) antioxidant pathway, and reduced liver UA synthesis by inhibiting the expression of UA synthesis protein XOD in rat model. In addition, tetrahydroalstonine alleviated inflammation via inhibiting PI3K/Akt/NF-κB signaling pathway, with cornuside and hydroxygenkwanin enhanced renal tubule UA transport capacity by regulating the translations of XDH and HRP genes, all of which protected HUA-rat kidney from inflammatory infiltration damage, and reduced serum urea nitrogen (BUN), creatinine (CRE) and UA levels.
Conclusions These findings indicates that Cor can alleviate HUA by enhancing liver-renal-intestine UA metabolism, inhibiting inflammatory responses of liver-renal-intestine as well as providing hepatorenal protection.
These findings indicates that Cor can alleviate HUA by enhancing liver-renal-intestine UA metabolism, inhibiting inflammatory responses of liver-renal-intestine as well as providing hepatorenal protection.
作者机构：
[Yi Wang; Pengjie Wang; Peipei Wu] Department of Nutrition and Health, China Agricultural University, Beijing, 100083, China;[Jinghua Yu] College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, 300457, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China;Mengniu Hi-Tech Dairy Products (Beijing) Co., Ltd, Beijing, 101100, China;[Menghui Wang; Jinhui Yang] Inner Mongolia Mengniu Dairy (Group) Co., Ltd, Hohhot, 011500, China
通讯机构：
[Jinghua Yu] C;College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, 300457, China
摘要：
Creaming is an issue in UHT milk due to extended storage periods and higher storage temperatures. Therefore, this study aims to unravel the key interfacial molecules and core structure behind the stability of UHT milk fat globules . Raw milk from Holstein, Jersey, and goat breeds, which differ in interfacial components, was selected. The fat globules were standardized for particle size using the UHT system, and stability analysis was performed using Turbiscan to establish high, medium, and low stability fat globule models. Omics analysis revealed that the abundance of 3 interfacial proteins (butyrophilin, adipophilin, and xanthine oxidoreductase) and 2 interfacial phospholipids (phosphatidylserine and sphingomyelin) significantly increased as the fat globule stability increased. Support vector machine was used to identify key interfacial molecules. The results showed that the high abundance of butyrophilin, adipophilin, phosphatidylserine , and sphingomyelin contributed to fat globule stability. Molecular docking and CHARMM-GUI further determined that butyrophilin interacted with adipophilin mainly through hydrogen bonds (ΔG = −47.8 kcal/mol and interaction area = 3088.4 Å 2 ). The abundance of this complex in high-stability fat globules was 2.2 times greater than that in medium-stability fat globules and 4.9 times greater than that in low-stability fat globules, respectively. We hypothesize that the butyrophilin-adipophilin complex may anchor both the inner monolayer and the outer bilayer, forming the backbone of the entire interface. Therefore, this study first reveals that the key molecules contributing to the stability of UHT milk fat globules are butyrophilin, adipophilin, phosphatidylserine, and sphingomyelin, with the butyrophilin-adipophilin complex serving as the core structure.
Creaming is an issue in UHT milk due to extended storage periods and higher storage temperatures. Therefore, this study aims to unravel the key interfacial molecules and core structure behind the stability of UHT milk fat globules . Raw milk from Holstein, Jersey, and goat breeds, which differ in interfacial components, was selected. The fat globules were standardized for particle size using the UHT system, and stability analysis was performed using Turbiscan to establish high, medium, and low stability fat globule models. Omics analysis revealed that the abundance of 3 interfacial proteins (butyrophilin, adipophilin, and xanthine oxidoreductase) and 2 interfacial phospholipids (phosphatidylserine and sphingomyelin) significantly increased as the fat globule stability increased. Support vector machine was used to identify key interfacial molecules. The results showed that the high abundance of butyrophilin, adipophilin, phosphatidylserine , and sphingomyelin contributed to fat globule stability. Molecular docking and CHARMM-GUI further determined that butyrophilin interacted with adipophilin mainly through hydrogen bonds (ΔG = −47.8 kcal/mol and interaction area = 3088.4 Å 2 ). The abundance of this complex in high-stability fat globules was 2.2 times greater than that in medium-stability fat globules and 4.9 times greater than that in low-stability fat globules, respectively. We hypothesize that the butyrophilin-adipophilin complex may anchor both the inner monolayer and the outer bilayer, forming the backbone of the entire interface. Therefore, this study first reveals that the key molecules contributing to the stability of UHT milk fat globules are butyrophilin, adipophilin, phosphatidylserine, and sphingomyelin, with the butyrophilin-adipophilin complex serving as the core structure.
摘要：
This study investigated the release of taste-active peptides from soybean isolate catalyzed by proteases from Aspergillus flavus strains. The frequent presence of Leu at the C-terminus and the hydrophilic amino acids at the N-terminus related to bitterness and umami taste of hydrolysates, respectively. Detailly, H40650 demonstrating high umami, low bitterness, and a high peptide yield. During the hydrolysis of H40650, umami taste changes were associated with the content of peptides <1 kDa and 28 umami peptides, while bitterness was linked to the content of peptides <3 kDa and 30 bitter peptides. Additionally, most taste-active peptides were derived from the 7S globulin α subunit and 11S globulin G2. The protease produced by CGMCC 40650 has marked specificity for the region of 281–333 amino acids in protein P0D15, which contained 24.53 % umami amino acids. These findings could offer new insights into the preparation of taste-active peptides.
This study investigated the release of taste-active peptides from soybean isolate catalyzed by proteases from Aspergillus flavus strains. The frequent presence of Leu at the C-terminus and the hydrophilic amino acids at the N-terminus related to bitterness and umami taste of hydrolysates, respectively. Detailly, H40650 demonstrating high umami, low bitterness, and a high peptide yield. During the hydrolysis of H40650, umami taste changes were associated with the content of peptides <1 kDa and 28 umami peptides, while bitterness was linked to the content of peptides <3 kDa and 30 bitter peptides. Additionally, most taste-active peptides were derived from the 7S globulin α subunit and 11S globulin G2. The protease produced by CGMCC 40650 has marked specificity for the region of 281–333 amino acids in protein P0D15, which contained 24.53 % umami amino acids. These findings could offer new insights into the preparation of taste-active peptides.
摘要：
The primary active compound in vine tea is dihydromyricetin (DMY), which has a longstanding history as a dietary supplement and traditional ethnic medicine. However, the precise molecular mechanism by which vine tea dihydromyricetin extract (VDMY) regulates glucolipid metabolic disorder remains unclear. In this study, we first assessed the effect of VDMY on various physiological parameters in db/db mice, followed by RNA sequencing (RNA-seq) to identify key signaling pathways affected by VDMY in liver tissues. We also examined the impact of VDMY on the liver's TLR4/MyD88/NF-κB and FOXO1 pathways using Western blotting. Our results showed that VDMY significantly reduced fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TGs), and low-density lipoprotein cholesterol (LDL-C), while increasing high-density lipoprotein cholesterol (HDL-C) levels. Additionally, VDMY enhanced the liver's antioxidant capacity by upregulating superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), while lowering malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), thus alleviating liver damage. RNA-seq analysis further revealed that VDMY influenced multiple biological processes, including transcription, glycolysis, gluconeogenesis, and redox reactions, suggesting that its effects may be mediated through the TLR4/MyD88/NF-κB and FOXO1 pathways. Additionally, Western blot analysis revealed that VDMY effectively downregulated the expressions of TLR4, MyD88, NF-κB, and FOXO1 proteins in the liver of db/db mice, indicating that VDMY could target these pathways to intervene glucolipid metabolism dysfunction.
摘要：
Limited sources of consortia/pure cultures that degrade chloramphenicol (CAP) and the incomplete biodegradation profiles of CAP hinder the remediation of CAP pollution. In this study, two CAP-degrading consortia (designated as CM and PM) were obtained after long-term acclimation, and Alcaligenaceae and Enterobacteriaceae enriched in CM and PM, respectively. Notably, Bordetella sp. C3, a new isolate belonging to the family Alcaligenaceae , was isolated from CM and capable of degrading 85.7 % 10 mg/L CAP at 30 ℃ and pH 7 in 10 d. The biotransformation of CAP by Bordetella sp. C3 was proposed as a detoxification process, including a novel initial degradation pathway: dechlorination of CAP into AP. Strain C3 can also function as a plant growth-promoting bacterium that solubilizes inorganic phosphate and produces siderophores and indole-3-acetic acid (IAA). This study expands our knowledge of the migration and transformation pathways of CAP and microbial community profiles during acclimatization.
Limited sources of consortia/pure cultures that degrade chloramphenicol (CAP) and the incomplete biodegradation profiles of CAP hinder the remediation of CAP pollution. In this study, two CAP-degrading consortia (designated as CM and PM) were obtained after long-term acclimation, and Alcaligenaceae and Enterobacteriaceae enriched in CM and PM, respectively. Notably, Bordetella sp. C3, a new isolate belonging to the family Alcaligenaceae , was isolated from CM and capable of degrading 85.7 % 10 mg/L CAP at 30 ℃ and pH 7 in 10 d. The biotransformation of CAP by Bordetella sp. C3 was proposed as a detoxification process, including a novel initial degradation pathway: dechlorination of CAP into AP. Strain C3 can also function as a plant growth-promoting bacterium that solubilizes inorganic phosphate and produces siderophores and indole-3-acetic acid (IAA). This study expands our knowledge of the migration and transformation pathways of CAP and microbial community profiles during acclimatization.
期刊：
Separation and Purification Technology,2025年:135441 ISSN：1383-5866
通讯作者：
Zhijian Tan
作者机构：
[Changyong Cai] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China;[Zhijian Tan] Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Hunan 410205, China;[Yunlan Chen; Zhonghai Tang] College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Zhijian Tan] I;Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Hunan 410205, China
摘要：
Rosa roxburghii Tratt ( R. roxburghii ) polysaccharides have important application value in the fields of food and medicine due to their significant antioxidant and hypoglycemic activities. However, conventional extraction methods have low efficiency and poor environmental friendliness. In this study, a sustainable and highly efficient approach for R. roxburghii Tratt extraction was developed using thermo-responsive ionic liquids (TRILs). The choline chloride/lithium bis(trifluoromethylsulfonyl)imide system exhibited the optimal performance, enabling facile polysaccharide recovery via temperature regulation. It was found that the response temperature of TRIL was between 20 and 80 °C. The optimized extraction process achieved a maximum polysaccharide yield of 212.0 mg/g. Changing the temperature can change the hydrophilicity and hydrophobicity of TRIL, leading to the enrichment of polysaccharides in the aqueous phase and the recovery of polysaccharides. Additionally, this TRIL displayed excellent reusability, retaining an extraction capacity of 195.0 mg/g (retention rate 91.9 %) after five cycles. Subsequent purification yielded three polysaccharide fractions (RTFP-T1, RTFP-T2, and RTFP-T3). RTFP-T3 displayed superior antioxidant activity, scavenging 97.13 % of ABTS and 79.79 % of DPPH radicals. This study not only provides innovative solutions for the sustainable extraction of Rosa roxburghii Tratt polysaccharides, but also lays the technical foundation for the industrial application of natural active polysaccharides.
Rosa roxburghii Tratt ( R. roxburghii ) polysaccharides have important application value in the fields of food and medicine due to their significant antioxidant and hypoglycemic activities. However, conventional extraction methods have low efficiency and poor environmental friendliness. In this study, a sustainable and highly efficient approach for R. roxburghii Tratt extraction was developed using thermo-responsive ionic liquids (TRILs). The choline chloride/lithium bis(trifluoromethylsulfonyl)imide system exhibited the optimal performance, enabling facile polysaccharide recovery via temperature regulation. It was found that the response temperature of TRIL was between 20 and 80 °C. The optimized extraction process achieved a maximum polysaccharide yield of 212.0 mg/g. Changing the temperature can change the hydrophilicity and hydrophobicity of TRIL, leading to the enrichment of polysaccharides in the aqueous phase and the recovery of polysaccharides. Additionally, this TRIL displayed excellent reusability, retaining an extraction capacity of 195.0 mg/g (retention rate 91.9 %) after five cycles. Subsequent purification yielded three polysaccharide fractions (RTFP-T1, RTFP-T2, and RTFP-T3). RTFP-T3 displayed superior antioxidant activity, scavenging 97.13 % of ABTS and 79.79 % of DPPH radicals. This study not only provides innovative solutions for the sustainable extraction of Rosa roxburghii Tratt polysaccharides, but also lays the technical foundation for the industrial application of natural active polysaccharides.
摘要：
This study investigated the efficacy of exosome-like nanoparticles derived from Camellia sinensis (CSENs), isolated using immunoaffinity (IM), ultracentrifugation (UC), and polyethylene glycol precipitation (PEG) methods, for suppressing heterocyclic amines (HAs) formation and enhancing sensory attributes in roasted chicken breast (marinated with 0.05–0.50 % CSENs). Results demonstrated that PEG-isolated CSENs at 0.50 % concentration achieved inhibition up to 100 % of key HAs including MeIQ, MeIQx, PhIP, Norharman, and Harman in both free and bound forms. Mechanistic studies revealed that CSENs attenuated HA generation through dual pathways including scavenging free radicals and suppressing reactive carbonyl intermediates. Notably, electronic tongue analysis confirmed that 0.50 % PEG-CSENs significantly enhanced umami, richness, and saltiness perception while reducing bitterness and astringency, with no adverse effects on textural properties. These findings establish Camellia sinensis exosomes as bifunctional agents for simultaneously improving chemical safety and organoleptic quality in thermally processed meats.
This study investigated the efficacy of exosome-like nanoparticles derived from Camellia sinensis (CSENs), isolated using immunoaffinity (IM), ultracentrifugation (UC), and polyethylene glycol precipitation (PEG) methods, for suppressing heterocyclic amines (HAs) formation and enhancing sensory attributes in roasted chicken breast (marinated with 0.05–0.50 % CSENs). Results demonstrated that PEG-isolated CSENs at 0.50 % concentration achieved inhibition up to 100 % of key HAs including MeIQ, MeIQx, PhIP, Norharman, and Harman in both free and bound forms. Mechanistic studies revealed that CSENs attenuated HA generation through dual pathways including scavenging free radicals and suppressing reactive carbonyl intermediates. Notably, electronic tongue analysis confirmed that 0.50 % PEG-CSENs significantly enhanced umami, richness, and saltiness perception while reducing bitterness and astringency, with no adverse effects on textural properties. These findings establish Camellia sinensis exosomes as bifunctional agents for simultaneously improving chemical safety and organoleptic quality in thermally processed meats.
摘要：
In response to microbial infestation during fruit storage, we developed a microbial self-destruction nanoplatform-cinnamaldehyde (CIN) nanoemulsions (NEs) based on zein-gum arabic (GA) nanoparticles inspired by the natural defense mechanisms of citrus oil gland, which enables sustained and precise release of CIN for long-term fruit preservation. Zein-2 % GA nanoparticles (ZGNP) are bound through hydrogen bonding and electrostatic interactions, demonstrating excellent emulsification stability. The prepared 5 % CIN-loaded NEs (5 %C-ZGNP NEs) exhibited high encapsulation efficiency (91.64 %) and outstanding sustained release properties (maintaining 38.52 % at 4 °C and 28.51 % at 25 °C after 30 d of storage), thereby enabling continuous and effective broad-spectrum antimicrobial activity. Additionally, co-incubation experiments with NEs and microorganisms validated the precise release of CIN and the microbial self-destruction. In the rapid growth phase of the microorganisms, enzymatically triggered hydrolysis of ZGNP by microbial secretions led to a precise and intelligent release of CIN, effectively triggering the microbial self-destruction process (self-destruction rate > 70 %), which successfully mimicked the natural defense mechanism of citrus oil gland. In vivo experiments on citrus and cherry tomatoes further validated the precise antimicrobial properties of NEs. Finally, the 5 %C-ZGNP NEs was prepared as a coating for fruit preservation, extending the shelf life of citrus and cherry tomatoes by 2-fold. The preservation effect was overall superior to the commercially available preservative, prochloraz. The biomimetic intelligent nanoplatform designed in this study enables the sustained and precise release of essential oils, effectively inhibiting microbial infestation and thereby extending the shelf life of fruits. This provides a new strategy for the development of “intelligent” responsive fruit preservation coating materials.
In response to microbial infestation during fruit storage, we developed a microbial self-destruction nanoplatform-cinnamaldehyde (CIN) nanoemulsions (NEs) based on zein-gum arabic (GA) nanoparticles inspired by the natural defense mechanisms of citrus oil gland, which enables sustained and precise release of CIN for long-term fruit preservation. Zein-2 % GA nanoparticles (ZGNP) are bound through hydrogen bonding and electrostatic interactions, demonstrating excellent emulsification stability. The prepared 5 % CIN-loaded NEs (5 %C-ZGNP NEs) exhibited high encapsulation efficiency (91.64 %) and outstanding sustained release properties (maintaining 38.52 % at 4 °C and 28.51 % at 25 °C after 30 d of storage), thereby enabling continuous and effective broad-spectrum antimicrobial activity. Additionally, co-incubation experiments with NEs and microorganisms validated the precise release of CIN and the microbial self-destruction. In the rapid growth phase of the microorganisms, enzymatically triggered hydrolysis of ZGNP by microbial secretions led to a precise and intelligent release of CIN, effectively triggering the microbial self-destruction process (self-destruction rate > 70 %), which successfully mimicked the natural defense mechanism of citrus oil gland. In vivo experiments on citrus and cherry tomatoes further validated the precise antimicrobial properties of NEs. Finally, the 5 %C-ZGNP NEs was prepared as a coating for fruit preservation, extending the shelf life of citrus and cherry tomatoes by 2-fold. The preservation effect was overall superior to the commercially available preservative, prochloraz. The biomimetic intelligent nanoplatform designed in this study enables the sustained and precise release of essential oils, effectively inhibiting microbial infestation and thereby extending the shelf life of fruits. This provides a new strategy for the development of “intelligent” responsive fruit preservation coating materials.
摘要：
The potential of cold plasma (CP) treatment to promote the formation of amyloid fibrils (AFs) of ovalbumin (OVA) was evaluated relative to acidic heat fibrillation condition (pH = 2, 85 °C). Results indicated CP exhibited significant potential for promoting the formation of AFs of OVA, as evidenced by higher Thioflavin T (ThT) fluorescence intensity of AFs for CP-treated OVA (POVA) during the fibrillation process compared to AFs for native OVA under acidic heating conditions (NOVA). Long, curved and worm-like fibrils of CP-treated OVA with 2 min (POVA-2), which were thicker (3 nm in height) and longer (majority length ranging from 300 nm to 400 nm), were observed after 8 h of fibrillation, while irregular, short, worm-like fibrils of NOVA were detected. Unlike NOVA, which was only hydrolyzed into small peptides, the backbone of POVA-2 was cleaved into small peptide fragments, accompanied by the generation of dityrosine cross-linked aggregation/oligomer during fibrillation process, as evidenced by SDS-PAGE and dityrosine analysis. The result of hydrophobic, sulfhydryl and disulfide bonds, and dityrosine analysis showed hydrophobic interaction and dityrosine cross-links could be the main force driving the assembly and stacking of cross-β structures, leading to the formation of organized fibrillar structures for POVA-2, while only hydrophobic interaction was involved for NOVA. Additionally, analyses of emulsifying ability (EAI) and stability (ESI) of NOVA and POVA-2 displayed that both EAI and ESI of them were significantly improved, with POVA-2 exhibiting superior EAI compared to NOVA. Therefore, this study demonstrated CP is a promising technique to promote the generation of protein amyloid fibrils in a more efficient manner.
The potential of cold plasma (CP) treatment to promote the formation of amyloid fibrils (AFs) of ovalbumin (OVA) was evaluated relative to acidic heat fibrillation condition (pH = 2, 85 °C). Results indicated CP exhibited significant potential for promoting the formation of AFs of OVA, as evidenced by higher Thioflavin T (ThT) fluorescence intensity of AFs for CP-treated OVA (POVA) during the fibrillation process compared to AFs for native OVA under acidic heating conditions (NOVA). Long, curved and worm-like fibrils of CP-treated OVA with 2 min (POVA-2), which were thicker (3 nm in height) and longer (majority length ranging from 300 nm to 400 nm), were observed after 8 h of fibrillation, while irregular, short, worm-like fibrils of NOVA were detected. Unlike NOVA, which was only hydrolyzed into small peptides, the backbone of POVA-2 was cleaved into small peptide fragments, accompanied by the generation of dityrosine cross-linked aggregation/oligomer during fibrillation process, as evidenced by SDS-PAGE and dityrosine analysis. The result of hydrophobic, sulfhydryl and disulfide bonds, and dityrosine analysis showed hydrophobic interaction and dityrosine cross-links could be the main force driving the assembly and stacking of cross-β structures, leading to the formation of organized fibrillar structures for POVA-2, while only hydrophobic interaction was involved for NOVA. Additionally, analyses of emulsifying ability (EAI) and stability (ESI) of NOVA and POVA-2 displayed that both EAI and ESI of them were significantly improved, with POVA-2 exhibiting superior EAI compared to NOVA. Therefore, this study demonstrated CP is a promising technique to promote the generation of protein amyloid fibrils in a more efficient manner.
摘要：
This study explored a facile method for converting macadamia nutshells into bio-based nanomaterials, including cellulose nanofibers (CNFs) and lignin nanoparticles (LNPs), through deep eutectic solvent (DES) pretreatment coupled with a nanofabrication strategy. Comparisons of the physicochemical, morphological, and structural properties of the CNF and LNPs produced through acidic choline chloride/oxalic acid dihydrate (ACDES) and alkaline K(2)CO(3)/glycerol DES (ALDES) pretreatments were conducted using SEM, TEM, FTIR, XRD, TGA, GPC and 2D NMR. The CNFs obtained from ACDES pretreatment (ACCNFs) exhibited uniform and long filament-like structures with shorter whisker-like nanocrystals. Conversely, the CNFs produced with the ALDES pretreatment (ALCNFs) displayed irregular aggregates and nanofibril bundles. Additionally, the ACCNFs demonstrated higher crystallinity and contained small amounts of the oxalate half-ester compare to ALCNFs. During ACDES pretreatment, a large proportion of β-O-4, β-5, and β-β linkages in lignin disrupted and re-condensed to form lignin substructures, resulting in the assembly of cluster-like lignin nanoparticles pretreated with ACDES (ACLNP) aggregates. In contrast, lignin nanoparticles pretreated with ALDES (ALLNP) exhibited uniform nanospherical shapes because of the preservation of β linkages in lignin during the ALDES pretreatment. This work not only broadens the fabrication strategies for CNF and LNPs but also offered a promising approach for the valorization of lignocellulosic agricultural wastes.
作者机构：
[Jun Lv; Xiu-Bin Liu] College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan 528225, China;[Rana Muhammad Aadil] National Institute of Food Science and Technology, University of Agriculture, Faisalabad 38000, Pakistan;Changsha Innovation Institute for Food, Changsha 410128, China
通讯机构：
[Zhi-Wei Liu] C;College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan 528225, China<&wdkj&>Changsha Innovation Institute for Food, Changsha 410128, China
摘要：
The promotion of covalent grafting of seven structurally distinct polyphenols to β-Lactoglobulin (β-LG) via cold plasma (CP) treatment, and their influence on functionalities and antigenicity of β-LG were systematically investigated. Results indicated that covalent β-LG-polyphenol conjugates were generated after undergoing 45 s of CP treatment, with the highest (135.49 ± 1.87 μmol/g) and lowest (2.56 ± 1.43 μmol/g) polyphenol content in β-LG-(−)-epigallocatechin gallate (β-LG-EGCG) and β-LG-syringic acid (β-LG-SA) conjugates, respectively. This result clearly demonstrated that the binding capacity is directly related to the number of phenolic hydroxyl (-OH) groups in polyphenols. Multispectral analysis revealed that the β-LG's spherical conformation unfolded upon covalent grafting with polyphenols. Further analysis of functionalities and antigenicity revealed that improvement in functionalities and reduction in antigenicity of β-LG was positively correlated with the grafted polyphenol content. The β-LG conjugates modified with EGCG displayed a maximum decrease in antigenicity (58.40 ± 1.03 %), a nearly seven-fold increase in DPPH, and a five-fold increase in ABTS free radical-scavenging capabilities when compared to native β-LG. According to LC-MS/MS and immunoblot analysis, the primary cause of the decrease in antigenicity was the disruption of the linear IgE epitope in β-LG as a result of conjugated EGCG's masking and steric hindrance impact. These findings demonstrate that CP is a reliable method for promoting covalent conjugation between polyphenols and proteins. Moreover, the binding affinity was found to be positively correlated with the number of -OH groups present in the polyphenol, which is a key structural determinant for enhancing functional properties and for reducing the antigenicity of the resulting protein-polyphenol complexes.
The promotion of covalent grafting of seven structurally distinct polyphenols to β-Lactoglobulin (β-LG) via cold plasma (CP) treatment, and their influence on functionalities and antigenicity of β-LG were systematically investigated. Results indicated that covalent β-LG-polyphenol conjugates were generated after undergoing 45 s of CP treatment, with the highest (135.49 ± 1.87 μmol/g) and lowest (2.56 ± 1.43 μmol/g) polyphenol content in β-LG-(−)-epigallocatechin gallate (β-LG-EGCG) and β-LG-syringic acid (β-LG-SA) conjugates, respectively. This result clearly demonstrated that the binding capacity is directly related to the number of phenolic hydroxyl (-OH) groups in polyphenols. Multispectral analysis revealed that the β-LG's spherical conformation unfolded upon covalent grafting with polyphenols. Further analysis of functionalities and antigenicity revealed that improvement in functionalities and reduction in antigenicity of β-LG was positively correlated with the grafted polyphenol content. The β-LG conjugates modified with EGCG displayed a maximum decrease in antigenicity (58.40 ± 1.03 %), a nearly seven-fold increase in DPPH, and a five-fold increase in ABTS free radical-scavenging capabilities when compared to native β-LG. According to LC-MS/MS and immunoblot analysis, the primary cause of the decrease in antigenicity was the disruption of the linear IgE epitope in β-LG as a result of conjugated EGCG's masking and steric hindrance impact. These findings demonstrate that CP is a reliable method for promoting covalent conjugation between polyphenols and proteins. Moreover, the binding affinity was found to be positively correlated with the number of -OH groups present in the polyphenol, which is a key structural determinant for enhancing functional properties and for reducing the antigenicity of the resulting protein-polyphenol complexes.
期刊：
Food Research International,2025年208:116272 ISSN：0963-9969
通讯作者：
Yi Wang<&wdkj&>Ran Wang<&wdkj&>Jie Luo
作者机构：
["Li, Zhixi; Fan, Xiankang; Cao, Yu] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410114, China;[Zhou, Hui] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410114, China. Electronic address: zhouhui@hunau.edu.cn;[Wang, Pengjie] Department of Nutrition and Health, China Agricultural University, Beijing 100083, China. Electronic address: wpj1019@cau.edu.cn;[Zhang, Hao] Department of Nutrition and Health, China Agricultural University, Beijing 100083, China. Electronic address: zhanghaocau@cau.edu.cn;[Wang, Yi] Department of Nutrition and Health, China Agricultural University, Beijing 100083, China. Electronic address: wangyi922217@126.com
通讯机构：
[Yi Wang; Ran Wang] D;[Jie Luo] C;Department of Nutrition and Health, China Agricultural University, Beijing 100083, China<&wdkj&>College of Food Science and Technology, Hunan Agricultural University, Changsha, 410114, China
关键词：
In vitro gastrointestinal digestion;Interfacial layers;Liposome;Milk membrane protein;Milk polar lipid;Proteoliposome
摘要：
The interfacial properties of lipid droplets are crucial for infant digestion, yet few studies have explored the impact of the layers of lipid droplets on infant digestion. In this study, four emulsions were prepared: one with milk polar lipids (MPL) serving as the monolayer interface of the lipid droplet (ME-PL), one with MPL and membrane-specific protein (MSP) as the co-monolayer interface of the lipid droplet (ME-Co), one with liposome featuring MPL as the bilayer interface of the lipid droplet (BE-PL), and one with proteoliposome containing MPL and MSP as the co-bilayer interface of the lipid droplet (BE-Co). Cryo-TEM was used to determine the number of lipid droplet interface layers, while confocal laser scanning microscopy confirmed the interfacial distribution. Compared to monolayer emulsions, bilayer emulsions exhibited greater stability. Furthermore, in vitro digestion experiments revealed that BE-Co released free fatty acids the fastest and in the largest amount. During gastric digestion, emulsions with co-components interfaces released fewer saturated fatty acids (SFA) compared to those containing only MPL in the interface, whereas bilayer emulsions released more SFA and polyunsaturated fatty acids (PUFA) than monolayer emulsions. During intestinal digestion, bilayer and co-interfacial emulsions released more SFA and less unsaturated fatty acids (USFA), with BE-Co releasing the highest percentage of SFA (87.11 ± 0.10 %) and the lowest percentages of USFA, specifically 2.95 ± 0.00 % PUFA. This study introduces a novel preparation method that uses a bilayer interface to simulate the human milk globule interface and explores the effect of different interface layers on lipid droplet characteristics, providing valuable insights for the development of infant food.
The interfacial properties of lipid droplets are crucial for infant digestion, yet few studies have explored the impact of the layers of lipid droplets on infant digestion. In this study, four emulsions were prepared: one with milk polar lipids (MPL) serving as the monolayer interface of the lipid droplet (ME-PL), one with MPL and membrane-specific protein (MSP) as the co-monolayer interface of the lipid droplet (ME-Co), one with liposome featuring MPL as the bilayer interface of the lipid droplet (BE-PL), and one with proteoliposome containing MPL and MSP as the co-bilayer interface of the lipid droplet (BE-Co). Cryo-TEM was used to determine the number of lipid droplet interface layers, while confocal laser scanning microscopy confirmed the interfacial distribution. Compared to monolayer emulsions, bilayer emulsions exhibited greater stability. Furthermore, in vitro digestion experiments revealed that BE-Co released free fatty acids the fastest and in the largest amount. During gastric digestion, emulsions with co-components interfaces released fewer saturated fatty acids (SFA) compared to those containing only MPL in the interface, whereas bilayer emulsions released more SFA and polyunsaturated fatty acids (PUFA) than monolayer emulsions. During intestinal digestion, bilayer and co-interfacial emulsions released more SFA and less unsaturated fatty acids (USFA), with BE-Co releasing the highest percentage of SFA (87.11 ± 0.10 %) and the lowest percentages of USFA, specifically 2.95 ± 0.00 % PUFA. This study introduces a novel preparation method that uses a bilayer interface to simulate the human milk globule interface and explores the effect of different interface layers on lipid droplet characteristics, providing valuable insights for the development of infant food.
通讯作者：
Abderrahmane Aït-Kaddour<&wdkj&>Rana Muhammad Aadil<&wdkj&>Zhi-Wei Liu
作者机构：
[Jing Wang] Hunan Food and Drug Vocational College Changsha China;[Jun-Xiang Liu; Zhi-Wei Liu; Xiu-Bin Liu] College of Food Science and Technology Hunan Agricultural University Changsha China;[Najla AlMasoud] Department of Chemistry, College of Science Princess Nourah Bint Abdulrahman University Riyadh Saudi Arabia;[Abderrahmane Aït-Kaddour] Université Clermont Auvergne, INRAE, VetAgro Sup UMRF Aurillac France;[Abderrahmane Aït-Kaddour] Department of Food Technology, Faculty of Agroindustrial Technology University of Padjadjaran Sumedang Jawa Barat Indonesia
通讯机构：
[Abderrahmane Aït-Kaddour] U;[Rana Muhammad Aadil] N;[Zhi-Wei Liu] C;National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan<&wdkj&>Université Clermont Auvergne, INRAE, VetAgro Sup, UMRF, Aurillac, France<&wdkj&>Department of Food Technology, Faculty of Agroindustrial Technology, University of Padjadjaran, Sumedang, Jawa Barat, Indonesia<&wdkj&>College of Food Science and Technology, Hunan Agricultural University, Changsha, China<&wdkj&>Guangdong Key Laboratory of Food Intelligent Manufacturing, Foshan University, Foshan, China<&wdkj&>Changsha Innovation Institute for Food, Changsha, China
关键词：
amyloid fibrils;cold plasma;high internal phase emulsions;pea protein isolates
摘要：
The potential of forming pea protein isolate (PPI) amyloid fibrils promoted by cold plasma (CP) treatment (40 kV; 0, 3, 5, and 7 min), heat treatment (85°C for 12 h), and its ability to stabilize high internal phase emulsions (HIPEs) was investigated. Results indicated that rod-like amyloid fibrils of PPI were successfully formed and promoted by CP treatment, with a 12 nm diameter and 132-261 nm average length, as confirmed by Thioflavin T (ThT) fluorescence spectroscopy, SEM, and TEM. The globular structure unfolding, cleavage of the backbone of PPI by CP treatment, release of the building block "β-strands" structure, and assembly of the building blocks into fibrils during the fibrillation process were confirmed by SDS-PAGE, FTIR, fluorescence spectroscopy, and hydrophobic analysis. Compared with native PPI, fibrous PPI exhibited a strong capacity for stabilizing HIPEs, and the ability to stabilize HIPEs was PPI fibrils length dependent. Compared with HIPEs stabilized by long fibrils (CP5PF-8 and CP5PF-12), the HIPEs prepared by short fibrils (CP5PF-2 and CP5PF-4) displayed better thermal stability. Moreover, CP5PF-stabilized HIPEs exhibited excellent ionic and storage stability, while PPI amyloid fibrils with shorter lengths (CP5PF-2 and CP5PF-4) showed superior performance compared with longer fibrils lengths (CP5PF-8 and CP5PF-12).
摘要：
Tea residues are rich in dietary fiber, most of which are insoluble dietary fiber (IDF). However, soluble dietary fiber (SDF) is reported to show a better health-promoting effect. In this paper, the Eurotium cristatum (E. cristatum) fermentation method was employed to prepare SDF from tea residues. The results showed that the yield of SDF in fermented SDF (FSDF) was higher than that in unfermented SDF (USDF). Meanwhile, an increased proportion of galactose and a looser microstructure were observed in FSDF. In addition, FSDF has more advantages than USDF in relieving colitis symptoms. FSDF is more effective in reversing weight loss, colon shortening, and tissue damage. Meanwhile, it has a better regulatory effect on the level of inflammatory factors (IL-6, IL-1β, TNF-α, and IL-10) and oxidative stress (CAT, T-SOD, and MDA). FSDF treatment more effectively restored gut microbiota composition toward normal parameters compared to USDF by upregulating Akkermanisa and Lachospiracae_NK4A136_group and downregulating Helicobacter and Alisitipes. In conclusion, fermentation treatment with E. cristatum contributed to the preparation efficiency and bioactive effect of SDF from tea residues. This study will provide a theoretical basis for the development and utilization of tea residues.
通讯机构：
[Li, QM; Xia, HP ] H;Hunan Agr Univ, Coll Food Sci & Technol, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Rapeseed Oil Nutr Hlth & Deep Dev Engn Techn, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词：
Acidic deep eutectic solvents;Phase transition;Waxy maize starch
摘要：
The effects of molar ratio and carboxylic acids' structure of deep eutectic solvents (DESs) on the treatment of waxy maize starch (WMS) were systematically investigated. FT-IR results of DESs discovered that shorter carbon chain acids exhibited stronger hydrogen bonds. Subsequently, DESs were utilized to treat WMS. Microscopy, DSC, and viscosity measurements indicated that the dissolution and gelatinization phase transitions of WMS occurred simultaneously in acid-DESs. Specifically, shorter carbon chains led to lower viscosity and more obvious dissolution. Notably, relatively high solubilities of 57.02 wt% (choline chloride/formic acid, CF) and 53.39 wt% (choline chloride/oxalic acid dihydrate, CO) were obtained for 6 h at 60 °C. Further characterization of regenerated WMS using SEM, XRD, FT-IR, and GPC showed that the granular and crystalline structures of WMS were disrupted, with a significant decrease in molecular weight. The extent of starch disruption/degradation increased as the acids' carbon chain decreased. Therefore, the treatment of WMS in acid-DESs was predominantly influenced by the length of the acids' carbon chain. Additionally, esterification reactions between WMS and carboxylic acids were detected in the CF and CO systems. These findings could provide a promising and efficient approach for starch treatment and structural analysis.
The effects of molar ratio and carboxylic acids' structure of deep eutectic solvents (DESs) on the treatment of waxy maize starch (WMS) were systematically investigated. FT-IR results of DESs discovered that shorter carbon chain acids exhibited stronger hydrogen bonds. Subsequently, DESs were utilized to treat WMS. Microscopy, DSC, and viscosity measurements indicated that the dissolution and gelatinization phase transitions of WMS occurred simultaneously in acid-DESs. Specifically, shorter carbon chains led to lower viscosity and more obvious dissolution. Notably, relatively high solubilities of 57.02 wt% (choline chloride/formic acid, CF) and 53.39 wt% (choline chloride/oxalic acid dihydrate, CO) were obtained for 6 h at 60 °C. Further characterization of regenerated WMS using SEM, XRD, FT-IR, and GPC showed that the granular and crystalline structures of WMS were disrupted, with a significant decrease in molecular weight. The extent of starch disruption/degradation increased as the acids' carbon chain decreased. Therefore, the treatment of WMS in acid-DESs was predominantly influenced by the length of the acids' carbon chain. Additionally, esterification reactions between WMS and carboxylic acids were detected in the CF and CO systems. These findings could provide a promising and efficient approach for starch treatment and structural analysis.
摘要：
Moso bamboo ( Phyllostachys edulis L.) shoots (MOBSs) are a highly valued food resource known for their distinctive flavor and nutritional benefits. However, their rapid growth leads to significant variations in quality across developmental stages. This study presents a novel multi-platform approach combining headspace-gas chromatography–ion mobility spectrometry (HS-GC-IMS), headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPM E -GC–MS), electronic nose (E-nose), and molecular docking to comprehensively characterize the dynamic changes in volatile organic compounds (VOCs) of MOBS at three growth stages (low, medium, and high). A total of 64 and 39 VOCs were identified by HS-GC-IMS and HS-SPME-GC–MS, respectively, among which 13 key compounds—1-hexanol, 1-pentanol, 3-methyl-1-butanol, ( E )-2-heptenal, 1-heptanol, ( E , E )-2,4-nonadienal, propyl isovalerate, benzaldehyde, 1-octen-3-ol, methyl salicylate, methyl hexanoate, decanal, and ( E )-2-octenol—were identified as potential biomarkers for discriminating growth stages. These compounds effectively reflected the dynamic evolution of MOBS flavor profiles and were primarily derived from amino acid catabolism and lipid oxidation pathways. Additionally, eight organic acids and 17 free amino acids (FAAs) were quantified, showing significant dynamic changes during growth. Correlation network analysis revealed strong interrelationships among VOCs, organic acids, and FAAs, suggesting coordinated metabolic regulation during growth. Molecular docking demonstrated strong binding affinities between four signature VOCs—( E )-2-heptenal, benzaldehyde, 1-octen-3-ol, and methyl salicylate—and human olfactory receptors, providing a molecular basis for the characteristic aroma of MOBS. This work establishes the first growth-stage-resolved VOC profile of MOBS and highlights the value of integrated analytical techniques in plant volatilomics. The findings provide a scientific basis for precision harvesting and targeted processing of bamboo shoots to enhance sensory quality and promote value-added applications in the food industry.
Moso bamboo ( Phyllostachys edulis L.) shoots (MOBSs) are a highly valued food resource known for their distinctive flavor and nutritional benefits. However, their rapid growth leads to significant variations in quality across developmental stages. This study presents a novel multi-platform approach combining headspace-gas chromatography–ion mobility spectrometry (HS-GC-IMS), headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPM E -GC–MS), electronic nose (E-nose), and molecular docking to comprehensively characterize the dynamic changes in volatile organic compounds (VOCs) of MOBS at three growth stages (low, medium, and high). A total of 64 and 39 VOCs were identified by HS-GC-IMS and HS-SPME-GC–MS, respectively, among which 13 key compounds—1-hexanol, 1-pentanol, 3-methyl-1-butanol, ( E )-2-heptenal, 1-heptanol, ( E , E )-2,4-nonadienal, propyl isovalerate, benzaldehyde, 1-octen-3-ol, methyl salicylate, methyl hexanoate, decanal, and ( E )-2-octenol—were identified as potential biomarkers for discriminating growth stages. These compounds effectively reflected the dynamic evolution of MOBS flavor profiles and were primarily derived from amino acid catabolism and lipid oxidation pathways. Additionally, eight organic acids and 17 free amino acids (FAAs) were quantified, showing significant dynamic changes during growth. Correlation network analysis revealed strong interrelationships among VOCs, organic acids, and FAAs, suggesting coordinated metabolic regulation during growth. Molecular docking demonstrated strong binding affinities between four signature VOCs—( E )-2-heptenal, benzaldehyde, 1-octen-3-ol, and methyl salicylate—and human olfactory receptors, providing a molecular basis for the characteristic aroma of MOBS. This work establishes the first growth-stage-resolved VOC profile of MOBS and highlights the value of integrated analytical techniques in plant volatilomics. The findings provide a scientific basis for precision harvesting and targeted processing of bamboo shoots to enhance sensory quality and promote value-added applications in the food industry.
