关键词：
fermented digestion-promoting Chinese herbs;growing pig;growth properties;serum indicators;gut microbiota
摘要：
Simple Summary Developing new antibiotic substitutes to promote pig growth and health, as well as new feed to improve feed utilization and reduce manure production during the breeding process, have become important ways to solve the current dilemma and promote the pig industry. In this study, we have documented that adding a 2% and 3% digestion-promoting fermented Chinese herbal formula to feed effectively promoted the growth of growing pigs, improved serum immune and antioxidant activities, ameliorated the structure of colonic microbiota, and altered the composition of metabolites in colon contents. In particular, adding a 2% digestion-promoting fermented Chinese herbal formula to feed effectively reduced opportunistic pathogens and increased potential probiotics in the colonic microbiota. This study provides experimental data for the application and feed development of digestion-promoting fermented Chinese herbs for pig production and expands the understanding of the impact of fermented Chinese herbs on the host's gut microbiota and metabolism.Abstract (1) Background: the development of new antibiotic substitutes to promote pig growth and health has become an important way to solve the current dilemma and promote the pig industry. (2) Methods: to assess the effects of a fermented Chinese herbal (FCH) formula on the growth and immunity of growing pigs, 100 Duroc x Landrace x Yorshire three-way crossed growing pigs were randomly divided into control and treatment groups that were fed a basal diet, and a basal diet with 1% (group A), 2% (group B), and 3% (group C) FCH formulas, respectively. A sixty-day formal experiment was conducted, and their growth and serum indices, colonic microbiota, and metabolites were analyzed. (3) Results: the daily gain of growing pigs in groups A, B, and C increased by 7.93%, 17.68%, and 19.61%, respectively, and the feed-to-gain ratios decreased by 8.33%, 15.00%, and 14.58%, respectively. Serum immunity and antioxidant activities were significantly increased in all treatment groups. Particularly, adding a 2% FCH formula significantly changed the colon's microbial structure; the Proteobacteria significantly increased and Firmicutes significantly decreased, and the metabolite composition in the colon's contents significantly changed. (4) Conclusions: these results indicate that the FCH formula is a good feed additive for growing pigs, and the recommended addition ratio was 3%.
作者：
Su, Guirong;Li, Shuzhen;He, Zhiguo;Zhong, Hui;Sun, Wei
期刊：
Journal of Environmental Chemical Engineering,2023年11(6):111509 ISSN：2213-3437
通讯作者：
He, ZG;Zhong, H
作者机构：
[Sun, Wei; Li, Shuzhen; He, ZG; Su, Guirong; He, Zhiguo] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Su, Guirong] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[He, Zhiguo] Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;[Zhong, Hui; Zhong, H] Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
通讯机构：
[He, ZG ; Zhong, H ] C;Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
关键词：
Chalcopyrite;Ethyl xanthate;Adhesion;Biofilm formation;Atomic force microscopy
摘要：
Adhesion and biofilm formation play important roles in the bio-dissolution of metal sulfides, which can initiate dissolution and enhance bioleaching. Ethyl xanthate, a commonly used flotation reagent, remained in flotation concentrates of metal sulfides and may have an impact on the bioleaching of metal sulfides. Therefore, it is important to analyze the effect of ethyl xanthate on microbial initial adhesion and biofilm formation. The results showed that the adhesion percentage of Acidianus manzaensis on chalcopyrite decreased with increasing ethyl xanthate concentration after adsorption equilibrium, due to the toxicity of ethyl xanthate to A. manzaensis. However, a low concentration (0.01 mg/L) of ethyl xanthate had little effect on the growth of A. manzaensis. The hydrophobicity of chalcopyrite increased after the addition of ethyl xanthate, leading to an increase in total interaction energy and adhesion force. The presence of ethyl xanthate inhibited the early growth of biofilm and delayed the formation of microcolonies on chalcopyrite. While biofilm coverage was larger with the addition of ethyl xanthate than that of the control when biofilm was mature. Furthermore, in the presence of ethyl xanthate, more and larger microcolonies were observed on the chalcopyrite surface when biofilm was matured, resulting in an increased corrosion degree and more jarosite produced on the chalcopyrite surface. This study suggested that a low concentration (0.01 mg/L) of ethyl xanthate could promote the dissolution of chalcopyrite, which could be significant for bioleaching of flotation reagent-bearing flotation concentrates.
期刊：
Science of The Total Environment,2023年881:163465 ISSN：0048-9697
通讯作者：
Zhi Liu<&wdkj&>Junhua Chen
作者机构：
[Liu, Zhi; Pan, Jiafeng; Deng, Fang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Chen, Manjia; Pan, Jiafeng; Deng, Fang; Chen, Junhua] Guangdong Acad Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut C, Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Peoples R China.;[Liu, Chengshuai; Chen, Manjia] Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China.;[Liu, Chengshuai] Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550081, Peoples R China.
通讯机构：
[Zhi Liu] C;[Junhua Chen] N;National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
摘要：
Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that cause harmful effects on environmental safety and human health. There is an urgent need to develop an intelligent method for PCBs sensing. In this work, we proposed a logic gate biosensing platform for simultaneous detection of multiple PCBs. 2,3',5,5'-tetrachlorobiphenyl (PCB72) and 3,3',4,4'-tetrachlorobiphenyl (PCB77) were used as the two inputs to construct biocomputing logic gates. We used 0 and 1 to encode the inputs and outputs. The aptamer was used to recognize the inputs and release the trigger DNA. A catalytic hairpin assembly (CHA) module is designed to convert and amplify each trigger DNA into multiple programmable DNA duplexes, which initiate the trans-cleavage activity of CRISPR/Cas12a for the signal output. The activated Cas12 cleaves the BHQ-Cy5 modified single-stranded DNA (ssDNA) to yield the fluorescence reporting signals. In the YES logic gate, PCB72 was used as the only input to carry out the logic operation. In the OR, AND, and INHIBIT logic gates, PCB72 and PCB77 were used as the two inputs. The output signals can be visualized by the naked eye under UV light transilluminators or quantified by a microplate reader. Our constructed biosensing platform possesses the merits of multiple combinations of inputs, intuitive digital output, and high flexibility and scalability, which holds great promise for the intelligent detection of different PCBs.
摘要：
The complete mitochondrial genome of Brachyrhynchus triangulus Bergroth, 1889 was sequenced and annotated in the present study. It was a typical circular DNA molecule of 15,170 bp, with 37 genes and a control region. The percentages of A, C, G, and T nucleotides in the genome of B. triangulus were 41.1%, 17.4%, 11.9%, and 29.4%, respectively. Thirteen protein-coding genes (PCGs) start with a ATN codon or a TTG codon and terminate with a TAA codon or a TAG codon or a single T residue. With the exception of tRNA(Ser(AGN)) , each of the 22 tRNA genes had a clover-leaf structure and ranged in length from 62 to 69 bp. The length of lrRNA and srRNA was 1241 bp and 828 bp, respectively. The control region had a length of 708 bp and an A + T content of 74.6%. The sister relationship between B. triangulus and Brachyrhynchus hsiaoi is supported by the phylogenetic tree. Additionally, it proved the sister relationship between Mezirinae and Aneurinae, supporting the classical taxonomy of the Aradidae.
摘要：
Enterococcus faecium (E. faecium) is widely used in foods and is known as a probiotic to treat or prevent diarrhea in pets and livestock. However, the poor resistance of E. faecium to high temperature processing procedures limits its use. Strain domestication is a low-cost and effective method to obtain high-temperature-resistant strains. In this study, heat treatment was performed from 45 & DEG;C to 70 & DEG;C and the temperature was gradually increased by 5 & DEG;C every 3 days. After domestication, the survival rates of the high temperature adaptation strain RS047-wl under 65 & DEG;C water bath for 40 min was 11.5 times higher than WT RS047. Moreover, the saturated fatty acid (SFA) contents in cell membrane and the cell volume significantly increased in the RS047-wl. The combined transcriptomic, metabolomic, and proteomics analysis results showed a significant enhancement of cell wall and membrane synthesis ability in the RS047-wl. In conclusion, one of the main factors contributing to the improved high temperature resistance of RS047-wl was its enhanced ability to synthesize cell wall and membrane, which helped maintain normal cell morphology. Developing a high-temperature-resistant strain and understanding its mechanism enables it to adapt to high temperatures. This lays the groundwork for its future development and application.
摘要：
The rapid development of nanocellulose and hydrogels has contributed to the development of renewable smart material technologies in recent years. Based on the excellent renewable and biodegradable properties of nanocellulose and the advantages of hydrogels with a high water content, porosity, excellent stretchability and comparable quality to living tissue, nanocellulose hydrogels, which combine the benefits of both, have received significant attention as pioneering materials in the green chemistry revolution. Herein, we present a comprehensive and systematic classification of the nanocellulose hydrogels reported to date in terms of their design-application target relationships. By revealing the intrinsic link between the inherent advantages of nanocellulose and hydrogels, we focus on the applications of nanocellulose hydrogels in medicine, electricity, sensor, environmental governance, food and agriculture and highlight their design innovation advantages. In addition, from the perspective of fundamental issues and practical applications, we provide an in-depth analysis of the shortcomings of the current nanocellulose hydrogels based on four major aspects, including their preparation, platform, application and safety and provide corresponding constructive comments to indicate the future direction of their development. By revealing the intrinsic link between the inherent advantages of nanocellulose and hydrogels, we highlight the applications of nanocellulose hydrogels in medical treatment, electricity, sensor, environmental governance, food, and agriculture.
摘要：
Lily Fusarium wilt disease caused by Fusarium spp. spreads rapidly and is highly destructive, leading to a severe reduction in yield. In this study, lily (Lilium brownii var. viridulum) bulbs were irrigated after planting with suspensions of two Bacillus strains that effectively control lily Fusarium wilt disease to assess their effects on the rhizosphere soil properties and microbial community. A high-throughput sequencing of microorganisms in the rhizosphere soil was performed and the soil physicochemical properties were measured. The FunGuild and Tax4Fun tools were used for a functional profile prediction. The results showed that Bacillus amyloliquefaciens BF1 and B. subtilis Y37 controlled lily Fusarium wilt disease with control efficacies of 58.74% and 68.93%, respectively, and effectively colonized the rhizosphere soil. BF1 and Y37 increased the bacterial diversity and richness of the rhizosphere soil and improved the physicochemical properties of the soil, thereby favoring the proliferation of beneficial microbes. The relative abundance of beneficial bacteria was increased and that of pathogenic bacteria was decreased. Bacillus abundance in the rhizosphere was positively correlated with most soil physicochemical properties, whereas Fusarium abundance was negatively correlated with most physicochemical properties. Functional prediction revealed that irrigation with BF1 and Y37 significantly upregulated glycolysis/gluconeogenesis among metabolism and absorption pathways. This study provides insights into the mechanism by which two Bacillus strains with antifungal activity, BF1 and Y37, antagonize plant pathogenic fungi and lays the foundation for their effective application as biocontrol agents.
关键词：
gut microbiota;Lactiplantibacillus plantarum;faecal microbiota transplantation;serum metabolites
摘要：
Gut microbiota may have therapeutic effects on inflammatory bowel disease (IBD). Regulating intestinal microbiota through Lactiplantibacillus plantarum (L. plantarum) and faecal microbiota transplantation (FMT) is a novel approach to treating IBD. This study aimed to explore the effect of L. plantarum and FMT pretreatment in alleviating colitis in mice. Five groups of mice (n = 6 per group) were included: CON group, DSS group (dextran sodium sulphate-induced colitis mice), LP-DSS pretreatment group (colitis mice were given strain L. plantarum and 5% DSS), DSS-FMT group (mice pretreated with faecal microbiota transplantation were given 5% DSS), and LP-FMT pretreatment group (mice pretreated with faecal microbiota transplantation and L. plantarum were given 5% DSS). Serum metabolites and intestinal microbiota were analysed by 16S rRNA sequencing liquid chromatography-mass spectrometry (LC-MS). The results demonstrated that L. plantarum and FMT improved gut microbiota in mice by increasing Firmicutes and decreasing the Bacteroidetes. In the serum metabolomics analysis, there were 11 differential metabolites in the DSS-FMT and LP-FMT pretreatment groups, and these differential metabolites were mainly glycerophospholipids and sphingolipids. It is worth noting that Lachnospira and Lactobacillus were positively associated with 8 differential metabolites. These results suggest that L. plantarum and FMT can regulate intestinal microorganisms and serum metabolomics to alleviate inflammation.
期刊：
Plant Physiology and Biochemistry,2023年196:793-806 ISSN：0981-9428
通讯作者：
Liqun Rao<&wdkj&>Qiming Wang
作者机构：
[Zhang, Jinhao; Chen, Rong; Chen, Yanchao; Peng, Guoping; Du, Lihua; Zhu, Qianfeng; Wang, Qiming; Xu, Nan; Rao, Liqun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Zhang, Jinhao; Chen, Rong; Chen, Yanchao; Peng, Guoping; Du, Lihua; Zhu, Qianfeng; Wang, Qiming; Xu, Nan; Rao, Liqun] Hunan Agr Univ, Hunan Engn Lab Good Agr Practice & Comprehens Util, Changsha 410128, Peoples R China.;[Li, Waichin] Educ Univ Hong Kong, Dept Sci & Environm Studies, Hong Kong, Peoples R China.;[Wu, Chuan] Cent South Univ, Sch Met & Environm, Changsha, Peoples R China.
通讯机构：
[Liqun Rao; Qiming Wang] C;College of Bioscience and Biotechnology Hunan Agricultural University, Changsha, 410128, China<&wdkj&>Hunan Engineering Laboratory for Good Agricultural Practice and Comprehensive Utilization of Famous-Region Medicinal Plants, Hunan Agricultural University, Changsha, 410128, China
摘要：
Light has important effects on plant metabolism. However, the relationship between the chlorogenic acid (CGA) content and light in plants remains unclear. Here, we investigated the effects of shading treatment on gene expression and CGA content in Lonicera macranthoides Hand.-Mazz. (LM), a widely used medicinal plant. A total of 1891 differentially expressed genes (DEGs) were obtained in flower buds and 819 in leaves in response to light in shading treatment compared to the control sample by RNA-Seq. After shading treatment, the content of CGA in LM leaves decreased significantly by 1.78-fold, the carotenoid content increased, and the soluble sugar and starch contents significantly decreased. WGCNA and the expression of related genes verified by qRT-PCR revealed that CGA synthesis pathway enzyme genes form a co-expression network with genes for carbohydrate synthesis, photosynthesis, light signalling elements, and transcription factor genes (TFs) that affect the accu-mulation of CGA. Through a virus-induced gene silencing (VIGS) system and CGA assay in Nicotiana benthamiana (NB), we determined that downregulation of NbHY5 expression decreased the CGA content in NB leaves. In this study, we found that light provides energy and material for the accumulation of CGA in LM, and light affects the expression of CGA accumulation-related genes. Our results show that different light intensities have multiple effects on leaves and flower buds in LM and are able to coregulate LmHY5 expression and CGA synthesis.
摘要：
It has been reported that Arabidopsis chloroplast accD transcripts undergo RNA editing and that loss of accD-C794 RNA editing does not affect plant growth under normal conditions. To date, the exact biological role of accD-C794 editing has remained elusive. Here, we reveal an unexpected role for accD-C794 editing in response to heat stress. Loss of accD-C794 editing results in a yellow and dwarf phenotype with decreased chloroplast gene expression under heat stress, and artificial improvement of C794-edited accD gene expression enhances heat tolerance in Arabidopsis. These data suggest that accD-C794 editing confers heat tolerance in planta. We also found that treatment with the product of acetyl coenzyme A carboxylase (ACCase) could allay mutant phenotypic characteristics and showed that a mutation in theCAC3 gene for the α-subunit of ACCase was associated with dwarfism under heat stress. These observations indicate that defective accD-C794 editing may be intrinsic to reduced ACCase activity, thereby contributing to heat sensitivity. ACCase catalyzes the committed step of de novo fatty acid (FA) biosynthesis. FA content analysis revealed that unsaturated oleic (C18:1) and linoleic acids (C18:2) were low in the accD-C794 editing-defective mutant but high in the C794-edited accD-overexpressing plants compared with the wild type. Supplying exogenous C18:1 and C18:2 could rescue the mutant phenotype, suggesting that these FAs play an essential role in tolerance to heat stress. Transmission electron microscopy observations showed that heat stress seriously affected the membrane architecture in accD editing-defective mutants but not in accD-overexpressing plants. These results provide the first evidence that accD-C794 editing regulates FA biosynthesis for maintenance of membrane structural homeostasis under heat stress.
摘要：
The bacteria pathogen Xanthomonas oryzae pv. oryzae (X-oo) infects rice and causes the severe disease of rice bacteria blight. As the central regulator of the salic acid (SA) signaling pathway, NPR1 is responsible for sensing SA and inducing the expression of pathogen-related (PR) genes in plants. Overexpression of OsNPR1 significantly increases rice resistance to X-oo. Although some downstream rice genes were found to be regulated by OsNPR1, how OsNPR1 affects the interaction of rice-X-oo and alters X-oo gene expression remains unknown. In this study, we challenged the wild-type and OsNPR1-OE rice materials with X-oo and performed dual RNA-seq analyses for the rice and Xoo genomes simultaneously. In X-oo-infected OsNPR1-OE plants, rice genes involved in cell wall biosynthesis and SA signaling pathways, as well as PR genes and nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes, were significantly upregulated compared to rice variety TP309. On the other hand, X-oo genes involved in energy metabolism, oxidative phosphorylation, biosynthesis of primary and secondary metabolism, and transportation were repressed. Many virulence genes of Xoo, including genes encoding components of type III and other secretion systems, were downregulated by OsNPR1 overexpression. Our results suggest that OsNPR1 enhances rice resistance to Xoo by bidirectionally regulating gene expression in rice and X-oo.
通讯机构：
[Wang, Zheng; Kang, Jinhe] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan, China<&wdkj&>CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Hunan, China
摘要：
This study was conducted to determine the response of the reproductive hormones and the mTOR/AKT/PI3K pathway in the ovaries of postpartum dairy cows with dietary rumen-protected glucose (RPG). Twelve Holstein cows were randomly assigned to two groups (n = 6/group): the control group (CT) and the RPG group. Blood samples were collected on d 1, 7, and 14 after calving for the gonadal hormone assay. The expression of the gonadal hormones receptors and PI3K/mTOR/AKT pathways were detected using RT-PCR and Western blot. The RPG addition increased the plasma LH, E2, and P4 concentrations on d 14 after calving and upregulated the mRNA and protein expressions of the ER alpha, ER beta, 17 beta-HSD, FSHR, LHR, and CYP17A1 but downregulated StAR expression. Immunohistochemical analysis identified higher expressions of the FSHR and LHR in the ovaries of RPG-fed cows compared to CT cows. Furthermore, the protein expressions of p-AKT/AKT and p-mTOR/mTOR were significantly increased in the ovaries of RPG-fed cows compared to the CT group, but the addition of RPG did not alter the protein expression of p-PI3K/PI3K. In conclusion, the current results indicated that dietary RPG supplementation regulated gonadotropin secretion and stimulated expression of hormone receptors and the mTOR/AKT pathway in the ovaries of early postpartum dairy cows. RPG may be beneficial for the recovery of ovarian activity in post-calving dairy cows.
通讯机构：
[Hui Fu] E;Ecology Department, College of Resources & Environments, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha, People’s Republic of China
摘要：
Functional traits of individuals and not just species can regulate ecological interactions with biotic and abiotic environments, yet how individual-level functional diversity (FDind) mediates the environmental effects on community properties (e.g., interaction patterns, connectivity and productivity) remains largely unexplored. Here, we assembled 4432 individuals from 30 macrophyte species across 26 lakes in south China and measured six functional traits -shoot height, specific leaf area, lamina thickness, leaf dry mass content, stem diameter and stem dry mass content-for each individual. We estimated FDind for macrophyte community in each plot using trait probability density framework. Path analysis revealed that functional richness promoted negative interactions (the absolute value of negative: positive cohesion as proxy of community interaction patterns) and thus community productivity at lower total phosphorus (TP) and shallower water, while functional divergence/redundancy reduced negative interaction and thus community productivity at shallower water, lower TP and higher altitude. Functional evenness decreased community productivity at higher TP and deeper water. Functional dissimilarity reduced community connectivity (total cohesion) and thus community productivity at deeper water and higher altitude. Our results highlight that FDind could modify the environmental effects (from local to regional) on the interaction patterns, network connectivity and productivity of macrophyte community.
关键词：
Coumarins;Biosynthesis;O-Methyltransferase;Catalytic promiscuity and fidelity;Angelica decursiva
摘要：
Angelica decursiva, an important traditional medicinal plant, possesses a unique pharmacological activity. Its principal active ingredients are coumarins, including scopoletin, bergapten, and imperatorin. However, the enzymes catalyzing the critical step of coumarins biosynthesis pathway remain unidentified. This study initially screened 14 candidate O-methyltransferases (OMTs) through transcriptomics and metabolic determination. Combined with gene expression profile and biochemical assays, two OMTs (AdOMT1 and AdOMT2) were identified to be responsible for the O-methylation of coumarins in A. decursiva. AdOMT1 showed higher catalytic efficiency for bergaptol (Kcat/Km = 3123.70), while AdOMT2 exhibited higher substrate and catalytic pro-miscuity, allowing it to catalyze the methylation of various coumarins, phenylpropanes, and flavonoids. Based on molecular docking and site-specific mutagenesis determined that His126/Asn132, Phe171/Phe177, Trp261/ Trp267, and Asn312/Ile317 were the key catalytic residues of AdOMT1 and AdOMT2 for the O-methylation of bergaptol and xanthotoxol. Further phylogenetic analysis confirmed the reasons for the catalytic functional differentiation of AdOMT1 and AdOMT2. This study provides a basis for exploring the coumarins O-methylation mechanism and plays a critical role in diversifying the structures used in coumarins drug discovery.
摘要：
The Arabidopsis pentatricopeptide repeat (PPR) proteins, required for accD RNA editing 1 (RARE1) and early chloroplast biogenesis 2 (AtECB2), each contain a DYW domain deemed essential for cytosine deamination at the accD-C794 RNA editing site in chloroplasts. Complementation assays using the rare1 mutant investigate the correlation between these PPRs and their respective DYW domain functions in RNA editing of accD-C794. The results demonstrate that the coding sequence of AtECB2 cannot replace that of RARE1. Moreover, rare1 mutants complemented with DYW-deleted RARE1 failed to recover the RNA editing of accD-C794 even in the presence of the highly similar DYW domain of the AtECB2 protein. These findings indicate that RARE1 and AtECB2 possess divergent roles in RNA editing, with specificity for accD-C794 directly attributable to DYW domain within RARE1. Structural modeling data suggest this functioning pertains to a local α-helical motif that residues slightly N-terminal to the consensus glutamate and CXXCH motif in the DYW domain for cytidine deamination during C-to-U editing by RARE1 that is absent within AtECB2.
作者机构：
[Zeng, Chao-Xi; Qin, Si; Chen, Zhong; Shi, Meng; He, Wen-Jiang] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[He, Wen-Jiang] Infinitus China Co Ltd, Guangzhou 510665, Peoples R China.;[Lv, Cheng-Hao; Qin, Si] Hunan Agr Univ, Coll Biol Sci & Technol, Changsha 410128, Peoples R China.;[Hou, De-Xing] Kagoshima Univ, Fac Agr, United Grad Sch Agr Sci, Kagoshima 8900065, Japan.
通讯机构：
[Si Qin] C;College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Author to whom correspondence should be addressed.
