通讯机构:
[Chunyun Guan; Mei Guan] C;College of Agriculture, Hunan Agricultural University, Hunan Branch of National Oilseed Crops Improvement Center, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Changsha 410128, China
关键词:
high-oleic-acid rapeseed;lncRNA-mRNA;lipid metabolism;seed development
摘要:
Abstract: A high oleic acid content is considered an essential characteristic in the breeding of high-quality rapeseed in China. Long-chain non-coding RNA (lncRNA) molecules play an important role in the plant’s growth and its response to stress. To better understand the role of lncRNAs in regulating plant reproductive development, we analyzed whole-transcriptome and physiological data to characterize the dynamic changes in lncRNA expression during the four representative times of seed development of high- and low-oleic-acid rapeseed in three regions. We identified 21 and 14 lncRNA and mRNA modules, respectively. These modules were divided into three types related to region, development stages, and material. Next, we analyzed the key modules related to the oil content and the oleic acid, linoleic acid, and linolenic acid contents with physiological data and constructed the key functional network analysis on this basis. Genes related to lipid metabolism, such as 3-ketoacyl-CoA synthase 16 (KCS16) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), were present in the co-expression network, suggesting that the effect of these genes on lipid metabolism might be embodied by the expression of these lncRNAs. Our results provide a fresh insight into region-, development-stage-, and material-biased changes in lncRNA expression in the seeds of Brassica napus. Some of these lncRNAs may participate in the regulatory network of lipid accumulation and metabolism, together with regulated genes. These results may help elucidate the regulatory system of lncRNAs in the lipid metabolism of high-oleic-acid rapeseed seeds. Keywords: high-oleic-acid rapeseed; lncRNA–mRNA; lipid metabolism; seed development
摘要:
Rice plants are highly sensitive to high-temperature stress, posing challenges to grain yield and quality. However, the impact of high temperatures on the quality of high-quality hybrid rice during the booting stage, as well as the differing effects of the booting and grain-filling stages on grain quality, are currently not well-known. Therefore, four high-quality hybrid rice were subjected to control (CK) and high-temperature stress during the booting (HT1) and grain-filling stages (HT2). Compared to the control, HT1 significantly reduced the spikelets panicle(-1) (16.1%), seed setting rate (67.5%), and grain weight (7.4%), while HT2 significantly reduced the seed setting rate (6.0%) and grain weight (7.4%). In terms of quality, both HT1 and HT2 significantly increased chalkiness, chalky grain rate, gelatinization temperature, peak viscosity (PV), trough viscosity (TV), final viscosity (FV), and protein content in most varieties, and significantly decreased grain length, grain width, total starch content, and amylose content. However, a comparison between HT1 and HT2 revealed that the increase in chalkiness, chalky grain rate, PV, TV, and FV was greater under HT2. HT1 resulted in a greater decrease in grain length, grain width, total starch content, and amylose content, as well as an increase in protein content. Additionally, HT1 led to a significant decrease in amylopectin content, which was not observed under HT2. Therefore, future efforts in breeding and cultivating high-quality hybrid rice should carefully account for the effects of high temperatures at different stages on both yield and quality.
作者机构:
[Zhou, Yanbiao; Yang, Yuanzhu] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.;[Zhao, Xinhui; Tang, Qianying; Zhang, Zhihui; Fu, Jun; Liu, Lan; Zhou, Yanbiao; Yang, Runqiu; Yang, Yuanzhu; Tang, Xiaodan] Yuan Longping High Tech Agr Co Ltd, Key Lab Southern Rice Innovat & Improvement, Minist Agr & Rural Affairs, Changsha 410001, Hunan, Peoples R China.;[Zhou, Yanbiao] South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China.;[Zhang, Zhihui; Yang, Yuanzhu] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Xinhui; Yang, Yuanzhu] Hunan Agr Univ, Coll Agron, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Yang, Yuanzhu; Zhou, Yanbiao] S;State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125, Hunan, China.;Key Laboratory of Southern Rice Innovation and Improvement, Ministry of Agriculture and Rural Affairs, Yuan Longping High-Tech Agriculture Co., Ltd., Changsha, 410001, Hunan, China.;College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.;College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China.
关键词:
STK;Salt stress;Rice;ROS scavenging;ABA
摘要:
BACKGROUND: Soil salinization is a major abiotic environmental stress factor threatening crop production throughout the world. Salt stress drastically affects the growth, development, and grain yield of rice (Oryza sativa L.), and the improvement of rice tolerance to salt stress is a desirable approach for meeting increasing food demand. Receptor-like cytoplasmic kinases (RLCKs) play essential roles in plant growth, development and responses to environmental stresses. However, little is known about their functions in salt stress. Previous reports have demonstrated that overexpression of an RLCK gene SALT TOLERANCE KINASE (STK) enhances salt tolerance in rice, and that STK may regulate the expression of GST (Glutathione S-transferase) genes. RESULTS: The expression of STK was rapidly induced by ABA. STK was highest expressed in the stem at the heading stage. STK was localized at the plasma membrane. Overexpression of STK in rice increased tolerance to salt stress and oxidative stress by increasing ROS scavenging ability and ABA sensitivity. In contrast, CRISPR/Cas9-mediated knockout of STK increased the sensitivity of rice to salt stress and oxidative stress. Transcriptome sequencing analysis suggested that STK increased the expression of GST genes (LOC_Os03g17480, LOC_Os10g38140 and LOC_Os10g38710) under salt stress. Reverse transcription quantitative PCR (RT-qPCR) suggested that four stress-related genes may be regulated by STK including OsABAR1, Os3BGlu6, OSBZ8 and OsSIK1. CONCLUSIONS: These findings suggest that STK plays a positive regulatory role in salt stress tolerance by inducing antioxidant defense and associated with the ABA signaling pathway in rice.
通讯机构:
[Cui, GX ; Liu, XY ] H;Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China.
关键词:
Cannabidiol;Electrochemical sensing;Gold nanoparticles;Carbon black
摘要:
Cannabidiol (CBD), a significant secondary metabolite of Cannabis sativa L., has pharmacological effects for the treatment of a variety of health conditions, including nerve protection, epilepsy, anti-inflammatory, anti-anxiety, and cancer, which have garnered increasing interest in recent years. Herein, an electrochemical sensing platform was constructed for ultrasensitive determination of CBD, on the base of a glassy carbon electrode (GCE) chemically modified with carbon black (CB) and gold nanoparticles (AuNPs) (CB/AuNPs/GCE). The morphology, structure and electrochemical performance of prepared CB/AuNPs/GCE were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), Ultraviolet-visible spectroscopy (UV-vis), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and electrochemical technologies. The effects of pH, scanning rate, enrichment time, enrichment potential and the speculated mechanism of cannabidiol reaction at modified electrode were studied in detail. Under the optimized conditions, the fabricated sensing platform exhibited a great linear response of 0.42 mu A mu M-1 in the concentration range of 0.25 similar to 50.0 mu M. The prepared CB/AuNPs/GCE showed long-term stability, good repeatability and high practicability in real samples of industrial hemp. The proposed sensor can be further enhanced for portable and rapid detection of plant secondary metabolites, providing a basis for food detection, agricultural development, and drug detection.
摘要:
Abstract: Gibberellin regulates plant growth, development, and metabolic processes. However, the underlying mechanism of the substantial effect of gibberellin on stem height and secondary metabolites in forage ramie is unclear. Therefore, this study combined transcriptomic and metabolomics analyses to identify the mechanisms regulating growth and secondary metabolite contents in forage ramie following exogenous gibberellin application. Exogenous gibberellin application significantly reduced the lignin content in the leaves but not in the stems. At the same time, gibberellin significantly increased the total flavonoid and chlorogenic acid contents in both the stems and leaves. In addition, 293 differentially expressed genes (DEGs) and 68 differentially expressed metabolites (DEMs) were identified in the leaves. In the stems, 128 DEGs and 41 DEMs were identified. The DEGs PER42, FLS, CYP75A, and PNC1 were up-regulated in the leaves, affecting phenylpropane metabolism. The joint analysis of the DEMs and DEGs revealed that the changes in the DEGs and DEMs in the leaves and stems improved the substrate efficiency in the phenol propane pathway and inhibited lignin synthesis in plants, thus shifting to flavonoid pathway synthesis. In conclusion, gibberellin treatment effectively reduces the lignin content in forage ramie while increasing the flavonoid and chlorogenic acid contents. These findings provide empirical and practical guidance for breeding for forage quality in ramie and the improvement and cultivation control of forage ramie. Keywords: gibberellin; transcriptome; metabolome; lignin; flavonoids
摘要:
Abstract: Heat stress (HS) has become one of the major abiotic stresses that severely constrain rice growth. Abscisic acid (ABA) plays an important role in plant development and stress response. However, the effect of different concentrations of exogenous ABA on HS tolerance in rice still needs to be further elucidated. Here, we found that high concentrations of exogenous ABA increased HS damage in seedlings, whereas 10−12 M ABA treatment increased fresh and dry weight under HS relative to mock seedlings. Our further data showed that, in response to HS, 10−5 M, ABA-treated seedlings exhibited a lower chlorophyll content, as well as transcript levels of chlorophyll biosynthesis and antioxidant genes, and increased the accumulation of reactive oxygen species (ROS). In addition, the transcript abundance of some heat-, defense-, and ABA-related genes was downregulated on 10−5 M ABA-treated seedlings under HS. In conclusion, high concentrations of exogenous ABA reduced the HS tolerance of rice seedlings, and this negative effect could be achieved by regulating the accumulation of ROS, chlorophyll biosynthesis, and the transcription levels of key genes in seedlings under HS. Keywords: rice (Oryza sativa L.); seedling; ABA; heat stress; reactive oxygen species
作者机构:
[Wu, Ya; Zeng, Zaohai; Xia, Rui; Liu, Yuanlong; He, Yehua; Chen, Chengjie; Xu, Jing; Chen, CJ] South China Agr Univ, Coll Hort, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510640, Guangdong, Peoples R China.;[Zeng, Zaohai; Xia, Rui; Liu, Yuanlong; Chen, Chengjie; Xu, Jing; Chen, CJ] South China Agr Univ, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr & Rural Affair, Guangzhou 510640, Guangdong, Peoples R China.;[Zeng, Zaohai; Xia, Rui; Liu, Yuanlong; Chen, Chengjie; Xu, Jing; Chen, CJ] South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510640, Guangdong, Peoples R China.;[Li, Jiawei] Jinan Univ, Guangdong Hongkong Macau Inst CNS Regenerat, Guangdong Key Lab Nonhuman Primate Res, Guangzhou 510632, Guangdong, Peoples R China.;[Wang, Xiao] Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Henan Joint Int Lab Crop MultiOm Res, Kaifeng 475004, Peoples R China.
通讯机构:
[Chen, CJ; Xia, R ] S;South China Agr Univ, Coll Hort, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510640, Guangdong, Peoples R China.;South China Agr Univ, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr & Rural Affair, Guangzhou 510640, Guangdong, Peoples R China.;South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou 510640, Guangdong, Peoples R China.
关键词:
TBtools-II;plugin;biological big data;BSA-seq
摘要:
Since the official release of the stand-alone bioinformatics toolkit TBtools in 2020, its superior functionality in data analysis has been demonstrated by its widespread adoption by many thousands of users and references in more than 5000 academic articles. Now, TBtools is a commonly used tool in biological laboratories. Over the past 3 years, thanks to invaluable feedback and suggestions from numerous users, we have optimized and expanded the functionality of the toolkit, leading to the development of an upgraded version-TBtools-II. In this upgrade, we have incorporated over 100 new features, such as those for comparative genomics analysis, phylogenetic analysis, and data visualization. Meanwhile, to better meet the increasing needs of personalized data analysis, we have launched the plugin mode, which enables users to develop their own plugins and manage their selection, installation, and removal according to individual needs. To date, the plugin store has amassed over 50 plugins, with more than half of them being independently developed and contributed by TBtools users. These plugins offer a range of data analysis options including co-expression network analysis, single-cell data analysis, and bulked segregant analysis sequencing data analysis. Overall, TBtools is now transforming from a stand-alone software to a comprehensive bioinformatics platform of a vibrant and cooperative community in which users are also developers and contributors. By promoting the theme"one for all, all for one", we believe that TBtools-II will greatly benefit more biological researchers in this big-data era.
期刊:
International Journal of Molecular Sciences,2023年24(3) ISSN:1422-0067
通讯作者:
Jianhua Zhang<&wdkj&>Nenghui Ye
作者机构:
[Chen, Yinke; Teng, Zhenning; Duan, Meijuan; Meng, Shuan; Ye, Nenghui] Hunan Agr Univ, Coll Agr, Changsha 410128, Peoples R China.;[Teng, Zhenning] Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China.;[Duan, Meijuan; Ye, Nenghui] Hunan Agr Univ, Hunan Prov Key Lab Rice Stress Biol, Changsha 410128, Peoples R China.;[Zhang, Jianhua] Hong Kong Baptist Univ, Dept Biol, Hong Kong 999077, Peoples R China.
通讯机构:
[Jianhua Zhang] D;[Nenghui Ye] C;College of Agriculture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Provincial Key Laboratory of Rice Stress Biology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Department of Biology, Hong Kong Baptist University, Hong Kong 999077, China
摘要:
Abstract: Light, temperature, water, and fertilizer are arguably the most important environmental factors regulating crop growth and productivity. Environmental stimuli, including low light, extreme temperatures, and water stresses caused by climate change, affect crop growth and production and pose a growing threat to sustainable agriculture. Furthermore, soil salinity is another major environmental constraint affecting crop growth and threatening global food security. The grain filling stage is the final stage of growth and is also the most important stage in cereals, directly determining the grain weight and final yield. However, the grain filling process is extremely vulnerable to different environmental stimuli, especially for inferior spikelets. Given the importance of grain filling in cereals and the deterioration of environmental problems, understanding environmental stimuli and their effects on grain filling constitutes a major focus of crop research. In recent years, significant advances made in this field have led to a good description of the intricate mechanisms by which different environmental stimuli regulate grain filling, as well as approaches to adapt cereals to changing climate conditions and to give them better grain filling. In this review, the current environmental stimuli, their dose–response effect on grain filling, and the physiological and molecular mechanisms involved are discussed. Furthermore, what we can do to help cereal crops adapt to environmental stimuli is elaborated. Overall, we call for future research to delve deeper into the gene function-related research and the commercialization of gene-edited crops. Meanwhile, smart agriculture is the development trend of the future agriculture under environmental stimuli. Keywords: light; temperature; water; fertilizer; saline–alkali stress; cereal crops; grain filling