期刊：
Small Ruminant Research,2007年72(1):1-10 ISSN：0921-4488
通讯作者：
Zhang, B
通讯机构：
[Zhang, B ] ;Human Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.
关键词：
small peptide;free amino acids;nitrogen equilibrium;digestibility;goat
摘要：
To study the effects of soybean small peptides (SSP) on nitrogen balance, nutrient digestibility and concentrations of glucose, ammonia and amino acids (AA) in portal veinous plasma (PVP) of goats, eight Xiangdong black goats were used in a 4 x 4 Latin square design. Goats were divided at random into four groups (two goats per group). Each group received the following four treatments in a different order over four treatment periods: SSP in the basic diet by feeding (SSPF), free AA (FAA) in the basic diet by feeding (FAAF), SSP by infusing through the duodenum fistula (SSPI), or FAA by infusing through the duodenum fistula (FAAI). Results showed that nitrogen retention (N-R) in SSPI and SSPF goats was greater than FAAF (P < 0.0 1) and FAAI (P < 0.05) goats, and NR in FAAl goats was greater than FAAF goats (P < 0.05). Apparent protein digestibility (aD(N)) in SSPI goats was significantly greater than FAAF (P < 0.01) and FAAI (P < 0.05) goats, and aD(N) in SSPF and FAAI goats was greater (P < 0.05) than FAAF goats, respectively. Moreover, apparent biological value of protein (aBV) in SSPI goats was greater by 48.71% (P < 0.0 1), 32.71 % (P <0.05) and 20.98% (P < 0.05) than FAAF, FAAl and SSPF goats, respectively, and aBV in SSPF goats was greater (P < 0.05) than FAAF goats. Regarding the digestibility of dietary nutrients, there were no significant differences in crude fat digestibility among the different treatments. However, ME digestibility in SSPI goats was greater by 14.59% (P < 0.05), 10.10% (P > 0.05) and 3.40% (P > 0.05) than FAAF, FAAl and SSPF goats, respectively. But, ME digestibility in FAAF goats was less (P > 0.05) than SSPF goats. Total AA digestibilities in SSPI, FAAI, SSPF and FAAF goats were 78.74%, 69.55%, 73.88% and 64.69%, respectively. By contrast, dietary crude fiber digestibilities in SSPF and FAAF goats were greater than SSPI or FAAI goats. Glucose levels in PVP of SSPI and FAAl goats were greater (P < 0.05) than SSPF and FAAF goats. Ammonia levels in PVP of SSPF and FAAF goats were greater (P < 0.05) than SSPI and FAAl goats. AA level in PVP of SSPI goats was significantly greater than FAAF (P < 0.0 1), FAAl (P < 0.05) and SSPF (P < 0.05) goats, and the levels in SSPF and FAAI goats were greater (P < 0.05) than FAAF goats. Our results suggested that SSP is more effective than FAA in promoting nitrogen balance and protein biological value, and increasing digestibility of dietary nutrients and levels of glucose and amino acids in PVP of goats. Supplying SSP or FAA by infusing through the duodenum fistula was more advantageous than that by feeding. (c) 2006 Elsevier B.V. All rights reserved.
摘要：
<正>变性梯度凝胶电泳（Denaturing Gradient Gel Electrophoresis,DGGE）是由Fisher和Lerman发明用于检测DNA突变的技术[1],1993年Muyzer等[2]首次将其用于分析土壤的微生物区系,成为检测微生物多样性,的一种有效方法。近年来开始运用于动物胃肠道微生物的研究,通过DGGE研究微生物数量及多样性,了解肠道微生物的结构和组成。本文就DGGE技术的原理、优缺点及其在畜牧业中的应用作一综述。
作者机构：
[李丽立; 印遇龙] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;[张彬] College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;[方热军] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Fang, R.] I;Institute of Subtropical Agriculture, Chinese Academy of Sciences, China
