Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles
作者：
Yao, Runxian;Zhao, Mingming;Zhong, Ling;Li, Yi;Li, Dazhi;...
期刊：
Pest Management Science ,2021年77(3):1214-1225 ISSN：1526-498X
通讯作者：
Deng, Ziniu;Ma, Xianfeng
作者机构：
[Deng, Ziniu; Ma, Xianfeng; Li, Dazhi; Yao, Runxian; Deng, ZN; Ma, XF; Zhao, Mingming] Hunan Agr Univ, Natl Ctr Citrus Improvement Changsha, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Deng, Ziniu; Ma, Xianfeng; Li, Dazhi; Yao, Runxian; Zhao, Mingming; Zhong, Ling] Hunan Agr Univ, Coll Hort, Changsha, Peoples R China.;[Li, Yi] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT USA.
通讯机构：
[Deng, ZN; Ma, XF] H;Hunan Agr Univ, Natl Ctr Citrus Improvement Changsha, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
关键词：
citrus;Bactrocera minax;odorant-binding protein;volatile organic compounds
摘要：
BACKGROUND: Bactrocera minax, one of the most important citrus pests, oviposits exclusively on citrus fruit. In the insect olfactory system, odorant-binding proteins (OBPs) facilitate the initial recognition role of host odor molecules. The aim of this study was to characterize the functional OBPs of B. minax and identify specific volatile organic compounds in the Citrus genus as OBP targets. RESULTS: BminOBP9 (BminGOBP99a), a closely related homolog of BdorGOBP99a, which reduces the egg-laying behavior of Bactrocera dorsalis through silencing technology, was cloned, expressed, and purified. The binding ability of BminOBP9 to 11 citrus volatiles was then examined using fluorescence competition binding assays (FCBA). The results demonstrated that BminOBP9 could bind to all tested citrus volatiles, as could BdorGOBP99a, ZcucGOBP99a, and ZtauGOBP99a. Interestingly, the binding ability of BminOBP9 was the strongest among the four, suggesting that BminOBP9 may have a function in the specific recognition of citrus volatiles. Furthermore, we aligned the above four proteins and found nine distinctive amino acid sites in BminOBP9. To identify the unique binding sites of BminOBP9, we produced the nine mutants using site-directed mutagenesis. Further FCBA showed that the binding ability of the nine mutants to citrus volatiles significantly reduced, and six of them (substitutes S24P, L36F, E53K, N68D, D112A, and S118R) had the weakest binding ability. CONCLUSION: The results demonstrated that BminOBP9 was the specific protein involved in the perception of citrus host volatiles by B. minax. Moreover, BminOBP9 could prove efficient in screening the candidate odors for pest management. © 2020 Society of Chemical Industry. © 2020 Society of Chemical Industry
语种：
英文
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枸橼种质对柑橘溃疡病的抗性鉴定
作者：
朱志媚;谭李梅;徐静;符红艳;胡哲;...
期刊：
中国果树 ,2021年(01):43-49+110-111 ISSN：1000-8047
作者机构：
湖南农业大学园艺园林学院,长沙410128;[胡哲] 国家柑橘改良中心长沙分中心;[谭李梅] 郴州市农业科学研究所;[马先锋; 邓子牛; 徐静; 龚蕾; 符红艳; 朱志媚; 王萍; 杨贵兵] 湖南农业大学园艺园林学院<&wdkj&>国家柑橘改良中心长沙分中心
关键词：
柑橘;枸橼;溃疡病;叶形态观察;活体接种;抗性鉴定
摘要：
柑橘溃疡病（Xanthomonas citri subsp. citri,Xcc）是严重阻碍柑橘产业发展的世界性检疫病害,防控困难,选育抗病品种为防治溃疡病的唯一有效办法。枸橼C-05是柑橘属独特的抗溃疡病种质,为抗性机理和抗病育种提供了种质基础。为了进一步评价枸橼C-05的抗性,另外收集了13份枸橼种质,对其进行溃疡病的离体和活体抗性评价。叶片形态学观察表明,14份枸橼种质存在较大的形态学差异。采用离体与活体注射接种不同浓度的柑橘溃疡病菌,根据叶片的感病症状、病斑周围黄色晕圈大小等典型溃疡病症状,综合评价枸橼类种质的抗病性。结果表明:枸橼种质之间对溃疡病的抗性存在极大差异,溃疡病的抗性评价综合病情指数显示枸橼C-05、园香橼、矮果香橼、美国枸橼、普通枸橼和印度大果综合病情指数较小,表现为极抗病或抗病,其他枸橼种质表现不同程度的感病性;枸橼种质的抗病或感病性与其叶片形态特征无关。枸橼C-05和5个抗病枸橼种质对柑橘溃疡病具有针对性的抗性,这对进一步研究抗病机理和抗病育种具有重要意义。
语种：
中文
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Decay control and quality of individually film-wrapped lemons treated with sodium carbonate
作者：
D'Aquino, Salvatore* ;Continella, Alberto;Gentile, Alessandra;Dai, Suming;Deng, Ziniu;...
期刊：
Food Control ,2020年108:106878 ISSN：0956-7135
通讯作者：
D'Aquino, Salvatore
作者机构：
[D'Aquino, Salvatore; Palma, Amedeo] CNR, Inst Sci Food Prod, Traversa Crucca 3, Sassari, Italy.;[Continella, Alberto; Gentile, Alessandra] Univ Catania, Dept Agr Food & Environm, Catania, Italy.;[Dai, Suming; Deng, Ziniu; Gentile, Alessandra] Hunan Agr Univ, Natl Ctr Citrus Improvement Changsha, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[D'Aquino, Salvatore] C;CNR, Inst Sci Food Prod, Traversa Crucca 3, Sassari, Italy.
关键词：
Decay;Film-wrapping;Lemons;Sodium carbonate;Storage
摘要：
The efficacy of 3-min-dip treatments with 3% sodium carbonate solutions at 20 or 50 degrees C was tested on lemons individually, not hermetically, sealed within low density polyethylene (LDPE) bags. Before sealing, treated lemons were rinsed (SCR) or not rinsed (SC) with fresh water. SC treatment almost completely inhibited green mold (Penicillium digitatum) development in wound-artificially inoculated lemons both wrapped and not wrapped after 15 d at 5 degrees C followed by 7 d at 20 degrees C or in those stored for 10 d at 20 degrees C. In SCR lemons, decay control was inferior, especially in wrapped lemons treated at 20 degrees C. Similar results were achieved with sound fruit inoculated 24 h before the treatments and stored for 30 d at 20 degrees C. SC and, at a less extent, SCR treatments increased dramatically mass loss of un-wrapped fruit compared to control fruit, whereas in wrapped fruit overall mass loss was almost completely inhibited. Wrapping the fruit in LDPE bags allowed to maintained fruit freshness and firmness and chemical quality along the whole storage time, regardless the treatments.
语种：
英文
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Substantial Equivalence of a Transgenic Lemon Fruit Showing Postharvest Fungal Pathogens Resistance
作者：
Muccilli, Vera;Vitale, Alessandro;Sheng, Ling;Gentile, Alessandra* ;Cardullo, Nunzio;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2020年68(12):3806-3816 ISSN：0021-8561
通讯作者：
Gentile, Alessandra
作者机构：
[Cardullo, Nunzio; Tringali, Corrado; Muccilli, Vera] Univ Catania, Dept Chem Sci, I-95125 Catania, Italy.;[Oliveri, Cinzia; La Rosa, Rosa; Distefano, Gaetano; Gentile, Alessandra; Di Guardo, Mario; La Malfa, Stefano; Vitale, Alessandro] Univ Catania, Dept Agr Food & Environm, I-95123 Catania, Italy.;[Deng, Ziniu; Sheng, Ling; Gentile, Alessandra] Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Peoples R China.
通讯机构：
[Gentile, Alessandra] U;[Gentile, Alessandra] H;Univ Catania, Dept Agr Food & Environm, I-95123 Catania, Italy.;Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Peoples R China.
关键词：
Agronomy;Citrus fruits;Cloning;Fungi;Genes;Metabolites;Botrytis cinerea;Chitinase genes;Citrus;NMR analysis;Penicillium italicum;Trichoderma sp;Nuclear magnetic resonance;Botrytis;Citrus;disease resistance;fruit;genetics;isolation and purification;microbiology;Penicillium;plant disease;transgenic plant;Botrytis;Citrus;Disease Resistance;Fruit;Penicillium;Plant Diseases;Plants, Genetically Modified
摘要：
The development of genetically modified (GM) crops speeds up the obtainment of novel varieties with improved agronomic characteristics. However, the risk evaluation of the use of GMs is mandatory before their release in the market. In this paper, an untargeted and comprehensive nuclear magnetic resonance-based metabolomic study was carried out on the peel and flesh of a transgenic lemon clone (E23) expressing the chit42 gene and exhibiting an increased tolerance to some pathogenic fungi and on its wild type. Results highlighted a substantial equivalence of the metabolomics profile of the transgenic clone compared to the wild type. In addition, an enhanced response of the E23 clone toward fungal pathogens affecting the postharvest management in lemon was evidenced. These results confirm the potential of genetic engineering for the punctual modification of specific agronomic traits without altering the whole pattern of metabolites and open new perspectives for a more sustainable and effective management of specific postharvest diseases in citrus. Copyright © 2020 American Chemical Society.
语种：
英文
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湖南产区甜橙果实品质分析
作者：
李大志;周嘉;刘曙东;蒋青岚;胡双双;...
期刊：
中国南方果树 ,2019年48(4):10-13 ISSN：1007-1431
作者机构：
湖南农业大学园艺园林学院/国家柑桔改良中心长沙分中心,长沙,410128;[邓子牛; 周嘉; 蒋青岚; 刘曙东; 李大志; 胡双双] 湖南农业大学
关键词：
甜橙;果实品质;湖南
摘要：
从湖南柑桔主产县(市)代表性柑桔生产基地果园采集12份普通甜橙(以冰糖橙为主)果实样品,17份脐橙(以纽荷尔脐橙为主)果实样品,包括13个甜橙品种,对单果质量、果实横纵径、种子数、出汁率、可食率、可溶性固形物含量、可滴定酸含量、固酸比和维生素C含量等品质指标进行测定和比较分析.结果 表明,冰糖橙、纽荷尔脐橙两个主栽品种在湖南各个产区果实品质性状稳定,湘南地区甜橙果实品质整体较好;卡拉卡拉脐橙、锦秀冰糖橙和橘湘元糖橙等品种的果实品质表现优异;早红脐橙、崀丰脐橙和园丰脐橙可以延长湖南脐橙的采收期.
语种：
中文
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柑橘新品种‘阿香2号’的选育
作者：
周嘉;夏绪平;胡双双;夏茜;唐帅;...
期刊：
果树学报 ,2019年36(4):529-532 ISSN：1009-9980
作者机构：
湖南农业大学园艺园林学院,长沙410128;国家柑橘改良中心长沙分中心,长沙410128;湖南阿香茶果食品有限公司,湖南益阳,413500;安化县移民开发局,湖南益阳,413500;[唐帅] 国家柑橘改良中心长沙分中心,长沙,410128
关键词：
柑橘;新品种;‘阿香2号’;芽变选种
摘要：
‘阿香2号’是从‘南丰蜜橘’芽变中选育的柑橘新品种。该品种树势强,适应性好,早结果,丰产,稳产。果实为扁圆形,果面橙黄色或橙色,风味浓郁,单果质量25~45 g,无核或少核。可溶性固形物含量(w,后同)12.8%,可滴定酸含量0.51%,维生素C质量浓度37.04 mg·100 mL~(-1),化渣性好,综合性状优良。在湖南安化11月上旬至中旬果实成熟。采用SSR分子标记对‘阿香2号’的遗传鉴定表明,‘阿香2号’与‘南丰蜜橘’在DNA水平上存在一定的差异,具备成为新品种的遗传基础,是一个稳定变异。‘阿香2号’适合在湖南省宽皮柑橘产区栽培,盛果期产量可达37 500 kg·hm~(-2)。
语种：
中文
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糖橙新品种‘橘湘元'的选育
作者：
Long G.;Huang H.;Zhang F.;Alessandra G.;Deng Z.;...
期刊：
果树学报 ,2019年36(3):388-391 ISSN：1009-9980
作者机构：
[Long G.; Huang H.; Zhang F.; Alessandra G.; Deng Z.; Li N.] Horticulture & Landscape Colege, Hunan Agricultural University, National Citrus Improvement Center Changsha Sub-Center, Hunan, Changsha, 410128, China
关键词：
柑橘;新品种;‘橘湘元’;芽变;无核;低酸
摘要：
‘橘湘元'是从埃及糖橙芽变中选育的无核糖橙新品种。果实圆球形,果形指数为0.95。平均单果质量173 g,大小较均匀,果面较光滑,有光泽,果肉橙黄色,平均每果实含0.4粒种子。可食率达77.09%,果实出汁率54.6%,可溶性固形物13.63%,可滴定酸0.14%,维生素C含量60.57 mg·100 mL~(-1)。‘橘湘元'在树形树势、叶片大小、花瓣宽度等方面与埃及糖橙均存在明显差异。果实生育期260 d,在湖南省永兴县11月中下旬成熟。SSR结果分析表明‘橘湘元'在300 bp处较‘埃及糖橙'多1条带,具有遗传特异性。‘橘湘元'可在山地和旱地种植,表现生长快,抗性较强。
语种：
中文
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Influence of the genetic background on the performance of molecular markers linked to seedlessness in table grapes
作者：
Bennici, Stefania;Di Guardo, Mario;Distefano, Gaetano* ;La Malfa, Stefano;Puglisi, Damiano;...
期刊：
Scientia Horticulturae ,2019年252:316-323 ISSN：0304-4238
通讯作者：
Distefano, Gaetano
作者机构：
[Puglisi, Damiano; Distefano, Gaetano; Bennici, Stefania; Arcidiacono, Fabio; Nicolosi, Elisabetta; Gentile, Alessandra; Di Guardo, Mario; La Malfa, Stefano] Univ Catania, Dipartimento Agr Alimentaz & Ambiente, I-95123 Catania, Italy.;[Ferlito, Filippo] Consiglio Ric Agr & Anal Econ Agr, Ctr Ric Olivicoltura Frutticoltura & Agrumicoltur, Corso Savoia 190, I-95024 Acireale, CT, Italy.;[Deng, Ziniu; Gentile, Alessandra] Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Distefano, Gaetano] U;Univ Catania, Dipartimento Agr Alimentaz & Ambiente, I-95123 Catania, Italy.
关键词：
Grape breeding;SCAR;SSR;Vitis vinifera (L.)
摘要：
One of the leading goals in novel breeding programs on table grapevine is the set-up of novel seedless varieties. Seedlessness has a strong impact on consumers’ choices representing an important added economic value compared to traditional cultivars. Breeding in tree crops greatly benefits from the use of molecular markers associated to a trait of interest. Nevertheless, one of the main limitations hampering marker assisted breeding is the lack of marker validation on multiple genetic backgrounds. To this extent, we evaluated the efficiency of four markers linked to seedlessness on 97 F 1 genotypes derived from 16 cross combinations obtained through in vitro embryo rescue. Our results confirmed that the four markers were associated to seedlessness even though all, to a different extent, showed marker-trait inconsistencies. Marker efficiency was then tested on the different genetic backgrounds composing the entire germplasm providing a more acurate overview of the marker performances when the genetic background changes. Overall, the most efficient markers were VMC7f2 and p3_VvAGL11 even though their effectiveness changed remarkably from one genetic background to another. This work reports a marker validation assay on a germplasm created to encompass the actual allelic variability employed by breeders. The results could represent a valuable tool to guide the choice of the best markers to assist selection, a choice that should be based, more than on the absolute efficiency of the marker, on a careful evaluation of its performance on the genetic backgrounds in analysis. © 2019 Elsevier B.V.
语种：
英文
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基于16S rDNA与多拷贝基因hyvI/hyvII的柑橘黄龙病菌PCR检测体系比较
作者：
陈岳文;李娜;李芳;肖翠;姚润贤;...
期刊：
Journal of Plant Protection ,2019年46(1):251-252 ISSN：0577-7518
作者机构：
湖南农业大学园艺园林学院,长沙,410128;[肖翠; 李娜; 姚润贤; 邓子牛; 李芳; 陈岳文] 湖南农业大学
关键词：
基于16S rDNA;多拷贝基因hyvI/hyvII;柑橘黄龙病菌
摘要：
柑橘黄龙病是由α变形菌纲细菌黄龙病菌耐热性亚洲种(Candidatus Liberibacter asiaticus,CLas)引起的柑橘毁灭性病害,世界柑橘产量居前三的中国、巴西、美国近年受灾严重.目前对CLas的分子检测主要靶点基因序列有16S rDNA、β-操纵子和外膜蛋白基因(Boyé,2015),但检出率及灵敏度相对较低,而利用多拷贝基因序列检测黄龙病的研究较少.为进一步提高检出率和灵敏度,本研究拟比较基于CLas的16S rDNA序列和多拷贝基因hyvⅠ/hyvⅡ所设计引物的灵敏度,建立柑橘黄龙病的高效检测体系,以期在实际应用中快速、灵敏、可靠地检测柑橘黄龙病.
语种：
中文
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柑橘品种鉴定的SSR标记开发和指纹图谱库构建
作者：
李益;马先锋;唐浩;李娜;江东;...
期刊：
中国农业科学 ,2018年51(15):2969-2979 ISSN：0578-1752
作者机构：
湖南农业大学园艺园林学院, 长沙, 410128;农业部科技发展中心, 北京, 100122;中国农业科学院柑桔研究所, 重庆, 400712;广西特色作物研究院, 广西, 桂林, 541004;[李益; 马先锋; 李娜; 龙桂友; 李大志; 邓子牛] 湖南农业大学园艺园林学院, 长沙, 410128
关键词：
柑橘;毛细管电泳;品种鉴定;DNA指纹图谱库
摘要：
【目的】筛选出一套SSR核心引物,用于构建柑橘品种的DNA指纹图谱库,为柑橘种苗认证、品种登记和植物新品种保护提供技术支撑。【方法】以柑橘属8个主要栽培种类为试材进行PCR扩增,扩增产物经琼脂糖凝胶电泳检测,筛选出多态性丰富、扩增条带稳定的引物对部分参试品种进行PCR扩增,扩增产物通过变性聚丙烯酰胺凝胶电泳进行进一步的引物筛选;筛选得到的引物进行荧光标记,并选用部分柑橘品种进行PCR扩增,扩增产物利用基因分析仪检测,分别计算各引物的PIC值、等位基因数目,选取一套多态性丰富的适合进行荧光毛细管电泳检测的SSR引物组合,进而对所有参试品种进行分析,构建柑橘品种的DNA指纹图谱库,同时利用筛选的SSR标记对参试品种进行鉴定。【结果】初期以柑橘属8个种的代表品种(太田椪柑、沙田柚、沅江酸橙、大红甜橙、邓肯葡萄柚、枸橼、费米耐劳柠檬和墨西哥来檬)为材料,用362对SSR引物进行PCR扩增,扩增产物经4%琼脂糖凝胶电泳检测,初步筛选出80对种间多态性高、扩增稳定的SSR引物;进一步从8个种类里选择不同类型的64个柑橘品种,用上述筛选出的80对SSR引物进行PCR扩增,扩增产物经6%变性聚丙烯酰胺凝胶电泳检测,再筛选出60对品种类型间多态性高、扩增稳定、条带清晰的SSR引物,并分别进行荧光标记;另以168份柑橘品种为材料对上述60对SSR引物进行PCR扩增,经荧光毛细管电泳检测,依据各引物的PIC值、等位基因数目、峰图读取难易程度、扩增稳定性及染色体位置等,最终筛选出21对SSR引物作为指纹图谱库构建的核心引物。为消除不同仪器、不同试验批次等引起的误差,为21对SSR引物各主要等位变异选择相应的参照品种。根据各引物标记的荧光颜色及扩增片段大小进行合理组合,21对SSR引物可分成5组进行多重电泳,共采集500份柑橘品种的DNA指纹。利用SSR标记,有111份品种仅用1对引物即可鉴别,86份品种用2对引物组合即可鉴别,24份品种用3对引物组合即可鉴别,另外279份品种虽无法单独一一鉴别,但可分为87个小组,组内品种无法区分,组间品种易鉴别。【结论】利用筛选出的21对SSR核心引物,构建了包含500份柑橘品种的DNA指纹图谱库,筛选出部分品种的特异标记,可以鉴别221份柑橘品种和87个品种组合,构建了基于毛细管电泳平台的柑橘SSR分子标记品种鉴定体系。
语种：
中文
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湖南临武柚产业发展现状与对策
作者：
***书;周嘉;郭永芳;邱彬;夏丽华;...
期刊：
全文版:农业科学 ,2018年(1):162-164
作者机构：
湖南省临武县农业综合服务中心;湖南农业大学园艺园林学院;国家柑橘改良中心长沙分中心;湖南省临武县气象局
关键词：
临武柚;现状;对策
摘要：
在分析临武的土地资源区位优势及临武柚产业发展现状及存在问题的基础上,就如何提高临武柚特色产业竞争力提出如下对策:制定临武柚中长期发展规划,优化临武柚产业规模生产;以绿色果园为核心,建立生态果园;健全技术服务网络,普及绿色高效栽培技术;布局市场营销网络,搭建销售平台,提高品牌的知名度;培植龙头企业和特色产业协会,促进供销一体化;发挥临武柚地理标志的作用,实现产品精包装。
语种：
中文
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冰糖橙果实品质综合评价量化模型的建立及应用
作者：
唐帅;易自力;李娜;谢一顾;邓子牛;...
期刊：
果树学报 ,2018年35(7):889-897 ISSN：1009-9980
作者机构：
湖南农业大学生物科学技术学院, 长沙, 410128;湖南农业大学园艺园林学院, 长沙, 410128;湖南农业大学, 国家柑橘改良中心长沙分中心, 长沙, 410128;[唐帅; 易自力] 湖南农业大学生物科学技术学院, 长沙, 410128;[李娜; 邓子牛; 李大志] 湖南农业大学园艺园林学院, 长沙, 410128 湖南农业大学, 国家柑橘改良中心长沙分中心, 长沙, 410128
关键词：
冰糖橙;量化模型;标准化值加权法;综合评价
摘要：
【目的】通过对湖南省柑橘各主产区冰糖橙及其优系连续3 a(年)的果实品质跟踪,建立果实品质综合评价的量化模型——标准化值加权法。【方法】该量化模型包括4个步骤:1、品质指标的趋向性调整;2、调整后的指标进行标准化值处理;3、根据各指标在专业上的重要性赋予权重;4、各指标标准化值与权重相乘后累积加分,得到果实品质的总分,进行排名。【结果】建立了标准化值加权法的量化模型。该模型能够客观准确对来自不同产地的30个冰糖橙品系进行品质排名,而非模糊判断;从产地来看,永兴县冰糖橙的品质整体为优;区域试验证明,冰糖橙优系‘农大2号'品质较‘农大1号'为优;麻阳纯甜系冰糖橙是可以大力推广的冰糖橙优良品系。【结论】该量化模型能够对冰糖橙果实品质进行准确的综合评价。从专业角度微调后,该量化模型亦适用于其他果树品种果实品质的综合评判,可以辅助育种。
语种：
中文
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A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants
作者：
Chen, Longzheng;Li, Wei;Katin-Grazzini, Lorenzo;Ding, Jing;Gu, Xianbin;...
期刊：
园艺研究(英文) ,2018年5(1):1-12 ISSN：2662-6810
通讯作者：
Li, Yi
作者机构：
[Chen, Longzheng; Wang, Ren; Gu, Xianbin; Li, Wei; Li, Yanjun; Li, Yi; McAvoy, Richard J.; Katin-Grazzini, Lorenzo; Lin, Xinchun] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA.;[Chen, Longzheng] Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing, Jiangsu, Peoples R China.;[Gu, Tingting; Li, Yi; Ding, Jing] Nanjing Agr Univ, Coll Hort, Nanjing, Jiangsu, Peoples R China.;[Gu, Tingting; Li, Yi; Ding, Jing] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China.;[Lin, Xinchun] Zhejiang Agr & Forestry Univ, State Key Lab Subtrop Silviculture, Hangzhou, Zhejiang, Peoples R China.
通讯机构：
[Li, Yi] U;[Li, Yi] N;Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA.;Nanjing Agr Univ, Coll Hort, Nanjing, Jiangsu, Peoples R China.;Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China.
关键词：
Genetic engineering;High-throughput screening;Molecular engineering in plants;Mutation;Next-generation sequencing;Gene editing
摘要：
Developing CRISPR/Cas9-mediated non-transgenic mutants in asexually propagated perennial crop plants is challenging but highly desirable. Here, we report a highly useful method using an Agrobacterium-mediated transient CRISPR/Cas9 gene expression system to create non-transgenic mutant plants without the need for sexual segregation. We have also developed a rapid, cost-effective, and high-throughput mutant screening protocol based on Illumina sequencing followed by high-resolution melting (HRM) analysis. Using tetraploid tobacco as a model species and the phytoene desaturase (PDS) gene as a target, we successfully created and expediently identified mutant plants, which were verified as tetra-allelic mutants. We produced pds mutant shoots at a rate of 47.5% from tobacco leaf explants, without the use of antibiotic selection. Among these pds plants, 17.2% were confirmed to be non-transgenic, for an overall non-transgenic mutation rate of 8.2%. Our method is reliable and effective in creating non-transgenic mutant plants without the need to segregate out transgenes through sexual reproduction. This method should be applicable to many economically important, heterozygous, perennial crop species that are more difficult to regenerate.
语种：
英文
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‘沙田柚’与枸橼杂交后代叶形态学性状遗传分析
作者：
胡哲;龙桂友;谭李梅;李大志;符红艳;...
期刊：
果树学报 ,2018年35(5):531-538 ISSN：1009-9980
作者机构：
湖南农业大学园艺园林学院, 长沙, 410128;国家柑橘改良中心长沙分中心, 长沙, 410128;广西特色作物研究院, 广西, 桂林, 541004;[胡哲; 龙桂友; 谭李梅; 李大志; 符红艳; 盛玲; 马先锋; 邓子牛] 湖南农业大学园艺园林学院, 长沙, 410128 国家柑橘改良中心长沙分中心, 长沙, 410128;[刘冰浩] 广西特色作物研究院, 广西, 桂林, 541004
关键词：
‘沙田柚’;枸橼;杂交育种;形态学性状;遗传变异
摘要：
【目的】探究‘沙田柚’与枸橼杂交后代叶形态学性状的遗传趋向性与相关性。【方法】以单胚、易感溃疡病品种‘沙田柚’为母本,与单胚、抗溃疡病种质枸橼C-05进行杂交,将得到的848株杂种后代作为研究对象,对其叶片形态学性状进行遗传趋向分析与偏相关分析。【结果】F_1代叶尖指数、叶形指数、翼叶宽度性状受母本的影响较大,叶柄长度性状、新叶颜色性状受父本的影响较大,叶尖指数、叶形指数表现为趋小变异,翼叶宽度表现为趋大变异,叶柄长度表现为趋中变异;有翼叶对无翼叶表现为显性,紫色新叶对绿色新叶表现为显性;新叶颜色与翼叶宽度、叶柄长度呈极显著负相关,与叶尖指数呈显著负相关,而与叶形指数呈极显著正相关,叶形指数与翼叶宽度呈极显著负相关,而翼叶宽度与叶柄长度呈极显著正相关。【结论】杂种F_1代叶尖指数、叶柄长度、叶形指数、翼叶宽度、新叶颜色等性状均有一定的遗传趋向性与相关性,可能为多基因控制的数量性状;新叶颜色性状可以作为类似杂种鉴定的形态学标记。本研究对于多年生木本植物杂交育种的性状选择、变异趋势研究有一定的指导作用。
语种：
中文
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铺层结构对三维中空夹层复合材料压缩性能的影响
作者：
周正亮;张守玉;陈同海;戚洪强;赵大娟
期刊：
工程塑料应用 ,2017年25(6):1243-1249 ISSN：1001-3539
作者机构：
[周正亮; 张守玉; 陈同海; 戚洪强; 赵大娟] 中材科技股份有限公司
关键词：
三维中空夹层复合材料;树脂含量;铺层结构;织物厚度;压缩强度
摘要：
采用手糊成型制备了双夹层三维中空夹层复合材料,重点研究了厚度为5 mm和8 mm的三维中空织物ZK5和ZK8铺层顺序对复合材料压缩强度的影响,并从树脂含量、织物厚度匹配性角度进行分析。结果表明,随树脂含量增加,三维中空夹层复合材料的压缩强度明显提高;双夹层三维中空夹层复合材料中,下层的树脂含量明显高于上层;ZK5和ZK8织物铺层顺序对双夹层中空复合材料的压缩强度影响较大,其中将ZK8置于ZK5下层时复合材料的压缩强度为ZK8置于ZK5上层时的1.7倍,即在三维中空夹层复合材料总厚度不变的情况下,将高厚度中空织物置于下层的结构明显优于将其置于上层的结构。通过这一概念可以在保证三维中空夹层复合材料整体厚度尺寸不变、质量不增加的条件下通过铺层结构的匹配设计,最大限度地提高复合材料的力学性能。
语种：
中文
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Corrigendum to A sequential treatment with sodium hypochlorite and a reduced dose of imazalil heated at 50° C effectively control decay of individually film-wrapped lemons stored at 20° C Postharvest Biology and Technology, 124 (2017) 75–84
作者：
D'Aquino, Salvatore* ;Dai, Suming;Deng, Ziniu;Gentile, Alessandra;Angioni, Alberto;...
期刊：
Postharvest Biology and Technology ,2017年133:121 ISSN：0925-5214
通讯作者：
D'Aquino, Salvatore
作者机构：
[D'Aquino, Salvatore; Palma, Amedeo] CNR, Inst Sci Food Prod, Traversa La Crucca 3, I-07100 Sassari, Italy.;[Dai, Suming; Deng, Ziniu; Gentile, Alessandra] Hunan Agr Univ, Natl Ctr Citrus Improvement Changsha, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Gentile, Alessandra] Univ Catania, Dept Agr Food & Environm, I-95123 Catania, Italy.;[Angioni, Alberto] Univ Cagliari, Dept Life Sci & Environm, Cagliari, Italy.;[De Pau, Luciano] Agr Res Agcy Sardinia, Agris Sardegna, Dept Wood & Fruit Tree, Via Mameli 126-D, I-09123 Cagliari, Italy.
通讯机构：
[D'Aquino, Salvatore] C;CNR, Inst Sci Food Prod, Traversa La Crucca 3, I-07100 Sassari, Italy.
摘要：
The authors regret to state that differently than reported in the original article a) Salvatore D'Aquino and Alessandra Gentile are the corresponding authors;b) Salvatore D'Aquino and Suming Dai contributed equally to the article.c) This work was supported by the National research Council of Italy, the science and technology project (2014WK2011), the “Overseas distinguished expert” project (2015021) of Hunan province, China and the S&T Project “Research and development of essential technologies for postharvest and conservation of citrus fruits”, 2014WK2011, of Hunan Province, China. Authors thank Mr. Domenico Mura for his technical support.The authors would like to apologise for any inconvenience caused. DOI of original article: http://dx.doi.org/10.1016/j.postharvbio.2016.10.006 © 2017
语种：
英文
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Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus
作者：
Li Fang;Dai Su-ming* ;Deng Zi-niu;Li Da-zhi;Long Gui-you;...
期刊：
农业科学学报(英文) ,2017年16(3):572-579 ISSN：2095-3119
通讯作者：
Dai Su-ming
作者机构：
[Deng Zi-niu; Li Da-zhi; Li Na; Long Gui-you; Li Yi; Li Fang; Dai Su-ming; Gentile, Alexandra] Hunan Agr Univ, Natl Ctr Citrus Improvement Changsha, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Li Yi] Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA.;[Gentile, Alexandra] Univ Catania, Dept Agr & Food Sci, I-95123 Catania, Italy.
通讯机构：
[Dai Su-ming] H;Hunan Agr Univ, Natl Ctr Citrus Improvement Changsha, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.
关键词：
citrus;Agrobacterium;transient expression efficiency;yellow fluorescent protein (YFP)
摘要：
Agrobacterium-mediated transient expression assays are a convenient alternative to stable expression because they are simple, easy to perform, and achieve gene expression rapidly. This study investigated the factors affecting transient gene expression efficiency in citrus by observing the cryo-sectioning of leaf samples under a laser confocal microscope. These factors included the composition of the infiltration buffer, the Agrobacterium cell density, the leaf development stage, the incubation temperature, and plant genotype. The highest transient expression level of yellow fluorescent protein (YFP) was detected in Mexican lime (Citrus aurantifolia) on the third day after the intermediate-aged leaves were infiltrated with the improved infiltration buffer 1 (15 mmol L−1 2-(N-morpholino) ethanesulfonic acid, 10 mmol L−1 MgCI2, and 200 pmol L−1 acetosyringone), which had an optical density of 0.8 and was incubated at 22°C. Additionally, this transient expression assay was applied to other citrus genotypes. Of note, trifoliate orange (Poncirus trifoliata) and kumquat (Fortunella obovate) had higher expression efficiency than other six genotypes of the Citrus genus. Our study provides research basis for the selection of optimization strategies in transient gene expression and improves the method for available genome investigation in citrus. © 2017 Chinese Academy of Agricultural Sciences
语种：
英文
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A sequential treatment with sodium hypochlorite and a reduced dose of imazalil heated at 50°C effectively control decay of individually film-wrapped lemons stored at 20°C
作者：
D'Aquino, Salvatore* ;Dai, Suming;Deng, Ziniu;Gentile, Alessandra;Angioni, Alberto;...
期刊：
Postharvest Biology and Technology ,2017年124:75-84 ISSN：0925-5214
通讯作者：
D'Aquino, Salvatore
作者机构：
[D'Aquino, Salvatore; Palma, Amedeo] CNR, Inst Sci Food Prod, Traversa La Crucca 3, I-07100 Sassari, Italy.;[Dai, Suming; Deng, Ziniu; Gentile, Alessandra] Hunan Agr Univ, Hort & Landscape Coll, Natl Ctr Citrus Improvement Changsha, Changsha 410128, Hunan, Peoples R China.;[Gentile, Alessandra] Univ Catania, Dept Agr Food & Environm, I-95123 Catania, Italy.;[Angioni, Alberto] Univ Cagliari, Dept Life Sci & Environm, Cagliari, Italy.;[De Pau, Luciano] Agris Sardegna, Agr Res Agcy Sardinia, Dept Wood & Fruit Tree, Via Mameli 126-D, I-09123 Cagliari, Italy.
通讯机构：
[D'Aquino, Salvatore] C;CNR, Inst Sci Food Prod, Traversa La Crucca 3, I-07100 Sassari, Italy.
关键词：
Decay;Imazalil;Individual-film-wrapping;Lemons;Sodium hypochlorite;Storage
摘要：
Storage of individual seal-packaged citrus fruit at room temperature in China is a very common practice that requires a pre-storage treatment with a high concentration of an effective fungicide as imazalil (IMZ) to prevent decay. In this study, lemons were washed with NaOCl (200 mg L-1) or not, treated with IMZ (50 or 1000 mg L-1) at 20 or 50 degrees C and individually wrapped with a 16-mu m thick extensible polyvinylchloride film (Film A) or two heat shrinkable polyolefinic films, thick 15 (Film B) or 19-mm (Film C). The sequential treatment with NaOCl and IMZ at 50 mg L-1 at 50 degrees C, was as effective as IMZ at 1000 mg L-1 at 20 degrees C in controlling Penicillium decay. Losses for decay in fruit wrapped with the two more permeable films (Film A and Film B) never exceeded 10%, while in those wrapped with Film C (the least permeable) peaked to 41%. All films reduced weight losses, which at the end of storage were 11% in fruit wrapped with Film A and below 4% in those wrapped with the other two films, while were 41% in unwrapped ones. After one week of storage, only 50% of unwrapped fruit were marketable whereas all wrapped fruit were still marketable after 8 weeks. Respiration as well as juice acetaldehyde and ethanol were slightly affected by the two more permeable films, while an abnormal production of CO2, acetaldehyde and ethanol occurred in those wrapped with film C. Changes in chemical parameters were relevant in fruit sealed with Film C and minor in those with Film A and Film B. Decay control and quality preservation of lemons stored at room temperature can be achieved for several weeks by a sequential treatment with NaOCl and a heated water emulsion of IMZ at 50 mg L (1), when fruit are wrapped with plastic films highly permeable to gases. (C) 2016 Elsevier B.V. All rights reserved.
语种：
英文
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柠檬自交后代抗柑橘溃疡病的离体鉴定
作者：
谭李梅;刘慧;朱志媚;周东;汤甜;...
期刊：
湖南农业科学 ,2017年(3):58-62 ISSN：1006-060X
作者机构：
[谭李梅; 邓子牛; 朱志媚] 湖南农业大学园艺园林学院,湖南 长沙 410128;[谭李梅; 邓子牛; 朱志媚] 国家柑橘改良中心长沙分中心,湖南 长沙 410128;湖南农业大学园艺园林学院,湖南 长沙,410128;[汤甜; 周东; 刘慧] 湖南农业大学
关键词：
溃疡病;柠檬自交后代;离体接种;抗性评价
摘要：
为鉴定柠檬自交后代对柑橘溃疡病的抗性,以柠檬鲜叶为材料,通过注射法和针刺法接种不同浓度溃疡病菌菌液,对114株柠檬自交后代群体进行离体鉴定,并以感病材料冰糖橙、抗病材料枸橼C-05作为对照。结果表明:柠檬自交后代对溃疡病抗性存在个体间差异;其中,注射接种法中表现为抗104和105 CFU/m L病原菌的植株有24株,针刺接种法中表现为抗108 CFU/m L病原菌的植株有11株;其中,有6株柠檬自交后代在2种鉴定方法中均被认定为抗病株。综合2种鉴定方法,114株柠檬自交后代中有29株对溃疡病表现出抗104 CFU/m L病原菌。
语种：
中文
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Seed, sugar and acid characteristics of ‘Zaomi’ Ponkan (Citrus reticulata Blanco), a spontaneous mutant of ‘Xinnu’
作者：
Li, Fei-fei;Gong, Lei;Deng, Zi-niu;Gentile, Alessandra;Long, Gui-you;...
期刊：
Scientia Horticulturae ,2017年225:707-715 ISSN：0304-4238
通讯作者：
Lu, Xiao-peng
作者机构：
[Gong, Lei; Deng, Zi-niu; Lu, Xiao-peng; Gentile, Alessandra; Long, Gui-you; Li, Da-zhi; Li, Fei-fei] Hunan Agr Univ, Coll Hort & Landscape, Changsha 4101289, Hunan, Peoples R China.;[Li, Fei-fei] Hunan Acad Agr Sci, Inst Hort, Changsha 410125, Hunan, Peoples R China.;[Gentile, Alessandra] Univ Catania, Dipartamento Sci Prod Agr Alimentari, I-95123 Catania, Italy.;[Peng, Ji-miao] Inst Citrus Sci, Jishou 416000, Xiangxi Autonom, Peoples R China.;[Li, De-jin] Inst Citrus Sci, Luxi 416100, Luxi County, Peoples R China.
通讯机构：
[Lu, Xiao-peng] H;Hunan Agr Univ, Coll Hort & Landscape, Changsha 4101289, Hunan, Peoples R China.
关键词：
'Zaomi' Ponkan;Seed;Male sterility;Self-incompatibility;Sugar;Acid
摘要：
Ponkan is one of the two easy-peeling mandarins grown in China, and ‘Xinnu’ Ponkan (Citrus reticulata Blanco) is a major cultivar in the industry. We selected a spontaneous mutant of ‘Xinnu’ named ‘Zaomi’ Ponkan. Compared to the wild type, ‘Zaomi’ Ponkan exhibited alternating agronomic traits comprising earlier colouring, thinner peel, fewer seeds and higher sugar content. Pollen analysis showed that ‘Zaomi’ produced a greater number of deformed, nonviable pollen grains. Experiments in self- and cross-pollination suggested that self-incompatibility of ‘Zaomi’ contributes to the seedlessness of ‘Zaomi’ × ‘Zaomi’. Fruit quality analysis demonstrated that ‘Zaomi’ was a high-sugar mutant that was not affected by pollinating combination. This study on the fruit characteristics of ‘Zaomi’ indicates that partial male sterility and self-incompatibility contributes to fewer or no seeds; natural pollination promotes the overall fruit quality of ‘Zaomi’ Ponkan. These results provide an explanation of the seed formation of ‘Zaomi’ Ponkan and supply information relevant to its commercial planting. © 2017
语种：
英文
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