Preparation of Magnetic Molecularly Imprinted Polymer (MMIP) Nanoparticles (NPs) for the Selective Extraction of Tetracycline from Milk
作者：
Gao, Wanru;Li, Jiayin;Li, Pao;Huang, Zhao;Cao, Yanan;...
期刊：
Analytical Letters ,2020年53(7):1097-1112 ISSN：0003-2719
通讯作者：
Liu, Xia
作者机构：
[Cao, Yanan; Li, Jiayin; Huang, Zhao; Gao, Wanru; Liu, Xia; Li, Pao] Hunan Agr Univ, Hunan Prov Key Lab Food Sci & Biotechnol, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Magnetic molecularly imprinted polymer;magnetic separation;milk;selective adsorption;tetracycline
摘要：
Core-shell magnetic molecularly imprinted polymer nanoparticles (MMIP NPs) were prepared for the adsorption and the isolation of tetracycline from food matrices. The obtained MMIP NPs were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffractometry, vibrating sample magnetometry, and thermogravimetric analysis. The adsorption properties of the MMIP NPs were systematically characterized by static, kinetic, and selective adsorption as well as reusability measurements. The MMIP NPs were shown to provide a high adsorption capacity, with a maximum value of 40.48 mg/g, satisfactory selectivity with an imprinting factor equal to 2.55, and direct magnetic separation. Moreover, the MMIP NPs were successfully applied to extract tetracycline from milk samples. Satisfactory recoveries from 91.14 to 104.52% were obtained. © 2019, © 2019 Taylor & Francis Group, LLC.
语种：
英文
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An assessment of the interaction for three Chrysanthemum indicum flavonoids and alpha-amylase by surface plasmon resonance
作者：
Li, Pao;Huang, Zhao;She, Yin;Qin, Si;Gao, Wanru;...
期刊：
FOOD SCIENCE & NUTRITION ,2020年8(1):620-628 ISSN：2048-7177
通讯作者：
Liu, Xia
作者机构：
[Cao, Yanan; She, Yin; Qin, Si; Huang, Zhao; Gao, Wanru; Liu, Xia; Li, Pao] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha, Hunan, Peoples R China.;[Qin, Si; Liu, Xia] Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha, Hunan, Peoples R China.;[Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Chrysanthemum indicum;flavonoids;interaction;surface plasmon resonance;alpha-amylase
摘要：
<jats:title>Abstract</jats:title><jats:p>This study evaluated the interaction of <jats:italic>Chrysanthemum indicum</jats:italic> (CI) flavonoids (luteolin, acacetin, and buddleoside) with α‐amylase. Surface plasmon resonance (SPR) assay showed their equilibrium dissociation constants (<jats:italic>K<jats:sub>D</jats:sub></jats:italic>) are 1.9695±0.12, 2.9240±0.20, and 3.2966±0.08mM at pH 6.0, respectively. Furthermore, their binding affinities were influenced by KCl, MgCl<jats:sub>2</jats:sub>, and CaCl<jats:sub>2</jats:sub>. Enzymatic kinetic studies revealed that three flavonoids exhibited noncompetitive α‐amylase inhibitory activity. The inhibitory sequence is luteolin>acacetin>buddleoside, which was in accordance with the results of binding affinity from SPR. 1,1‐diphenyl‐2‐picryl hydrazyl radical assay demonstrated that antioxidant activities of three flavonoids were inhibited significantly with α‐amylase. Meanwhile, the study reveals that hydroxyl on C′‐4, C′‐5, and C‐7 of flavonoids play an important role on the interaction of three flavonoids with α‐amylase. Also, SPR could be used as sensor for rapid screening inhibitors of α‐amylase and provide useful information for the application of <jats:italic>C.indicum</jats:italic> flavonoids in food and pharmaceutical area.</jats:p>
语种：
英文
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一种基于近红外光谱与化学计量学的绿茶快速无损鉴别方法
作者：
Li Pao;Shen Ru-jia;Li Shang-ke;Shan Yang;Ding Sheng-hua;...
期刊：
光谱学与光谱分析 ,2019年39(8):2584-2589 ISSN：1000-0593
通讯作者：
Du Guo-rong
作者机构：
[Shen Ru-jia; Jiang Li-wen; Du Guo-rong; Li Shang-ke; Li Pao; Liu Xia] Hunan Agr Univ, Hunan Prov Key Lab Food Sci & Biotechnol, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shan Yang; Ding Sheng-hua; Li Pao] Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Hunan, Peoples R China.;[Du Guo-rong] Shanghai Tobacco Grp Co Ltd, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China.
通讯机构：
[Du Guo-rong] H;[Du Guo-rong] S;Hunan Agr Univ, Hunan Prov Key Lab Food Sci & Biotechnol, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Shanghai Tobacco Grp Co Ltd, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China.
关键词：
近红外光谱;绿茶;连续小波变换;波长筛选;主成分分析
摘要：
绿茶是我国饮用范围最广、最受欢迎的一类茶叶。不同品种绿茶叶外观上差别较小,非专业人员难以直接用肉眼进行辨别。传统化学方法操作复杂、检测费用较高,对样品具有破坏性,无法实现快速无损分析。近红外光谱技术是一种简便、快速、无损、重现性好、可直接用于在线定性定量分析的新型分析技术。由于种植方式以及土壤、气候等生长环境的差异,不同品种绿茶叶中含氢基团有机物的种类和含量也不相同,因此可以通过扫描样品的近红外光谱,得到不同品种绿茶叶的特征信息,实现对不同品种绿茶叶的快速鉴别。研究提出了一种基于近红外光谱与化学计量学技术对不同品种绿茶的快速无损鉴别方法。使用近红外光谱仪得到了八个品种绿茶样品的光谱图,用主成分分析方法对不同品种绿茶样品数据进行了聚类分析。使用连续小波变换方法消除了光谱信号中的基线干扰,从而提升聚类效果。利用基于标准偏差与相对标准偏差的变量筛选方法进一步提高了聚类结果的准确性。结果表明:主成分分析后样品的第一主成分和第二主成分的方差贡献率之和在90%以上,可以选取前两个主成分进行聚类分析。直接采用原始数据进行聚类分析的准确率较低,难以满足应用需要;连续小波变换可以有效地消除光谱信号中的基线干扰。与直接使用原始光谱聚类结果相比,采用连续小波变换后聚类效果有显著提升,但依旧不能实现所有品种茶叶样品的准确鉴别。为了进一步提高方法的稳健性和分类结果的准确性,选取了标准偏差和相对标准偏差较大的波长数据进行聚类分析。在符合平均值大于1%的波长范围内,剔除标准偏差小于5‰的波长,进一步选择较大相对标准偏差值对应的波长点进行聚类分析。采用这种方式,可以仅使用几十个甚至是几个波长即可实现绿茶样品品种的准确聚类分析。波长筛选方法可以大大提高主成分分析结果的准确性,采用近红外光谱分析技术与化学计量学方法可以实现对不同品种绿茶的快速鉴别。经过对各个光谱吸收区域波长所对应官能团分析后,初步得出多酚、酰胺类以及氨基酸类物质的种类不同或含量差异是形成绿茶品种差异的重要原因。所提出的基于近红外光谱与化学计量学技术的方法具有较强的鉴别能力,为绿茶的快速无损分析提供了一种新手段。
语种：
中文
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苎麻籽总黄酮含量测定方法比较研究
作者：
赵振;刘霞;黄昭;李跑;邢虎成;...
期刊：
食品安全质量检测学报 ,2019年10(18):6195-6200 ISSN：2095-0381
作者机构：
湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,长沙 410128;湖南农业大学苎麻研究所,长沙 410128;湖南省种质资源创新与资源利用重点实验室,长沙 410128;[李跑; 邢虎成; 赵振; 曹亚男; 刘霞; 黄昭] 湖南农业大学
关键词：
苎麻籽;总黄酮;分光光度法
摘要：
目的 比较苎麻籽总黄酮含量的测定方法.方法 以芦丁和槲皮素为对照品, 比较直接测定法、NaNO2-Al3+-NaOH法、AlCl3法3种比色方法下苎麻籽(70%乙醇提取液)的紫外-可见光谱图, 并通过精密度、稳定性、干扰试验、加标回收率试验对所选方法进行评价.结果 芦丁和槲皮素均不适合作为直接测定苎麻籽总黄酮的对照品; NaNO2-Al3+-NaOH法最大吸收波长为500 nm, 但受某些非黄酮类物质干扰, 使得该方法下的总黄酮含量测定值虚高; AlCl3法以芦丁为对照品, 测定波长为401 nm, 在0.004～0.02 mg/mL范围内浓度与吸光度值成良好的线性关系, 相关系数 r2=0.9998, 该法受干扰小, 精密度高, 平均回收率为(99.46±4.16)%,显色后在20～60 min内吸光值稳定.结论 AlCl3法简单可行, 适用于苎麻籽提取物中总黄酮含量的测定.
语种：
中文
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《食品分析》创新型教学方法的改革与探索
作者：
罗凤莲;刘素纯;王燕;刘霞;李跑
期刊：
教育教学论坛 ,2019年(12):146-147 ISSN：1674-9324
作者机构：
湖南农业大学食品科学技术学院,湖南长沙,410128;[李跑; 王燕; 刘素纯; 刘霞; 罗凤莲] 湖南农业大学
关键词：
《食品分析》;创新型教学;教学改革
摘要：
《食品分析》课程是食品类专业的核心和基础课程,针对《食品分析》教学中存在的主要问题,提出了案例式、互动式、研讨式教学相结合的创新性教学方法改革和探索,对于食品相关专业的人才培养具有重要意义。
语种：
中文
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窗口竞争性自适应重加权采样策略的近红外特征变量选择方法
作者：
Li Pao;Zhou Jun;Jiang Li-wen;Liu Xia;Du Guo-rong*
期刊：
光谱学与光谱分析 ,2019年39(5):1428-1432 ISSN：1000-0593
通讯作者：
Du Guo-rong
作者机构：
[Jiang Li-wen; Du Guo-rong; Li Pao; Liu Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Du Guo-rong; Zhou Jun] Shanghai Tobacco Grp Co Ltd, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China.
通讯机构：
[Du Guo-rong] H;[Du Guo-rong] S;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Shanghai Tobacco Grp Co Ltd, Ctr Technol, Beijing Work Stn, Beijing 101121, Peoples R China.
关键词：
近红外光谱仪;化学计量学;窗口竞争性自适应重加权采样
摘要：
通过消除光谱中的冗余信息变量,挑选出代表样品性质的特征变量代替全谱建立定量模型,可以提高近红外分析结果的准确性。基于进化论中适者生存原理的竞争性自适应重加权采样(CARS)算法因具有计算速度快、筛选得到的特征波长少等优点,在近红外特征变量筛选方面得到了广泛的应用。然而该方法在计算过程中容易出现校正集和验证集结果不一致情况。这是因为算法过于强调校正集交叉验证结果,且并未考虑相邻变量之间的协同作用。为了建立更加稳健的变量筛选方法,通过结合“窗口”以及CARS算法的优势,提出了一种基于窗口竞争性自适应重加权采样(WCARS)策略的近红外特征变量筛选方法,并将其应用于复杂植物样品近红外光谱与其化学成分含量之间的建模分析。采用WCARS方法可以实现准确定量分析,且通过与竞争性自适应重加权采样(CARS)方法结果相比较,WCARS方法得到的校正集和预测集结果一致,在一定程度上减少了过拟合问题的出现。该策略能有效增强特征变量选择的稳健性,提高了定量模型的可信度,具有一定的应用价值。
语种：
中文
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A highly sensitive tetracycline sensor based on a combination of magnetic molecularly imprinted polymer nanoparticles and surface plasmon resonance detection
作者：
Gao, Wanru;Li, Pao;Qin, Si;Huang, Zhao;Cao, Yanan;...
期刊：
Microchimica Acta ,2019年186(9):637 ISSN：0026-3672
通讯作者：
Liu, Xia
作者机构：
[Cao, Yanan; Qin, Si; Huang, Zhao; Gao, Wanru; Liu, Xia; Li, Pao] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Surface plasmon resonance;Magnetic molecularly imprinted polymers nanoparticles;Tetracycline;Assay;Specific recognition
摘要：
A method is reported for in-situ detection of the antibiotic tetracycline (TC). It is based on a combination of extraction of TC by magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) and detection by surface plasmon resonance (SPR). The TC-captured MMIPs NPs were flowed over the surface of the SPR chip that was modified with mercaptoethylamine. The SPR signal undergoes a strong increase through the use of MMIPs NPs. It increases linearly in the 5.0–100pg·mL−1 TC concentration range, and the detection limit is as low as 1.0pg·mL−1 (at S/N = 3). This method shows selectivity to TC compared with structurally analogues. In order to demonstrate the power of the method, it was applied to the analysis of milk spiked with TC. These results were validated by comparing them to those of an enzyme-linked immunoassay. The average recovery is in the range of 95.7–104.6%. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.
语种：
英文
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Detection of Edible Plant Oil Adulteration by Triacylglycerol Profiles Using an Atmospheric Pressure Chemical Ionization Source and MS3 Ion Trap Mass Spectrometry
作者：
Wang, Xiupin;Li, Peiwu* ;Liu, Xia;Liu, Youqian;Zhang, Qi;...
期刊：
European Journal of Lipid Science and Technology ,2019年121(12):1900029-1-1900029-8 ISSN：1438-7697
通讯作者：
Li, Peiwu
作者机构：
[Wang, Xiupin; Li, Peiwu; Liu, Youqian; Zhang, Qi; Zhang, Liangxiao] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China;[Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Natl Reference Lab Agr Testing Mycotoxin, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Liu, Youqian; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Hubei, Peoples R China
通讯机构：
[Li, Peiwu] C;[Li, Peiwu] M;[Li, Peiwu] N;Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Hubei, Peoples R China. Natl Reference Lab Agr Testing Mycotoxin, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Hubei, Peoples R China.
关键词：
adulterated edible plant oil;multivariate statistical methods;RPLC-APCI-IT-MS;triacylglycerol profiles
摘要：
An adulteration of high-price oil has been an important concern in agri-food fraud problems. Triacylglycerols are the main components of edible plant oils, which play an important role as target for adulteration detections. The objective is to evaluate the utility of triacylglycerol profiles in the detection of adulteration in high priced oils. An effective method is established to detect adulteration of high priced oils based on triacylglycerol profiles and chemometrics. Triacylglycerol profiles of edible oils are analyzed by an APCI-MS3-IT-MS. All triacylglycerol compounds are quantified by normalization of the chromatographic peak area in selected reaction monitoring (SRM) mode based on MS3 fragment ion pairs, which are used to eliminate interference from the isobaric species of triacylglycerol. The results of the hierarchical cluster analysis (HCA) and random forest (RF) classification algorithm indicated that peanut, soybean, sesame, sunflower seed, and linseed oils are completely classified into five groups based on their triacylglycerol profiles. Finally, a recursive support vector machine (R-SVM) discriminant model is established, which can successfully identify adulteration of high priced oil with cheaper edible oils at a concentration of as low as 4% with an accuracy of 93.7%. Practical Applications: The triacylglycerol profiles of edible plant oils are analyzed by RPLC-APCI-IT-MS. The SRM scan mode based on MS3 fragment ion pairs can eliminate interference of triacylglycerol isomers. The discriminant model for adulterated high-price oils is established by R-SVM. HCA, and RF can classify the five kinds of edible plant oil. The triacylglycerol of five edible plant oils is analyzed by RPLC-APCI-MS3-IT-MS. The triacylglycerol data matrix is submitted using unsupervised (HCA) and supervised (RF) chemometric methods to classify five kinds of edible plant oil. A discriminant model established with the R-SVM can be used to identify adulterated linseed oil samples (>= 4%) with an accuracy of 93.7%.
语种：
英文
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磁性分子印迹聚合物在食品安全检测领域的研究与应用
作者：
高婉茹;黄昭;李跑;覃思;曹亚男;...
期刊：
食品研究与开发 ,2019年40(4):173-182 ISSN：1005-6521
作者机构：
湖南农业大学食品科学技术学院食品科学与生物技术湖南省重点实验室,湖南长沙,410000;[李跑; 覃思; 高婉茹; 刘霞; 曹亚男; 黄昭] 湖南农业大学
关键词：
分子印迹;磁性聚合物;样品前处理;食品安全;检测
摘要：
磁性分子印迹聚合物,是一类以磁性材料为载体,具有特定分子识别位点的聚合物。在外加磁场的作用下,该聚合物可直接选择性的富集分离分析物,故可有效的解决食品样品前处理耗时长、分离难等问题。将其与分析检测仪器联用,可实现食品中农兽药、违禁添加物等物质的快速、准确的检测。该文总结磁性分子印迹聚合物的基本制备方法,并讨论其优缺点;综述近年来国内外磁性分子印迹聚合物在食品安全检测领域的应用研究进展,并对其发展趋势作出展望。
语种：
中文
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近红外光谱结合化学计量法快速无损鉴别燕麦
作者：
李尚科;杜国荣;丁胜华;单杨;蒋立文;...
期刊：
食品与机械 ,2019年35(2):72-76 ISSN：1003-5788
作者机构：
湖南农业大学食品科学与技术学院食品科学与生物技术湖南省重点实验室,湖南 长沙,410128;湖南农业大学食品科学与技术学院食品科学与生物技术湖南省重点实验室,湖南 长沙 410128;上海烟草集团有限责任公司技术中心北京工作站,北京 101121;湖南省农业科学院 湖南省农产品加工研究所,湖南 长沙,410125;湖南省农业科学院 湖南省农产品加工研究所,湖南 长沙 410125
关键词：
近红外光谱技术;燕麦片;主成分分析法;连续小波变换;波长筛选
摘要：
提出了一种基于近红外光谱技术与化学计量学的燕麦无损鉴别方法。通过近红外光谱仪测定了5个品牌与劣质燕麦的光谱曲线,利用连续小波变换方法对光谱进行预处理,然后基于标准偏差与相对标准偏差的变量筛选方法筛选出具有代表的15个波数点,最后结合主成分分析法对不同燕麦样品快速鉴别。结果表明:连续小波变换可以有效地消除光谱中的背景干扰,提取光谱有效信息,波长筛选方法可以大大提高主成分分析结果的鉴别能力。通过结合近红外光谱分析技术与化学计量学方法,可对中国国产品牌、进口品牌和劣质燕麦进行准确鉴别。
语种：
中文
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A lysosome targetable fluorescent probe for palladium species detection base on an ESIPT phthalimide derivative
作者：
Shen, Youming;Zhang, Xiangyang;Wu, Yanyang* ;Zhang, Youyu;Liu, Xuewen;...
期刊：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY ,2018年205:66-71 ISSN：1386-1425
通讯作者：
Wu, Yanyang
作者机构：
[Shen, Youming; Zhong, Yutong; Zhang, Xiangyang; Chen, Yuandao; Liu, Xuewen] Hunan Univ Arts & Sci, Coll Chem & Mat Engn, Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Hunan Prov Cooperat Innovat Ctr Construct & Dev D, Changde 415000, Peoples R China.;[Wu, Yanyang] Hunan Agr Univ, Food Sci & Technol Coll, Changsha 410000, Hunan, Peoples R China.;[Zhang, Youyu; Li, Haitao] Hunan Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Wu, Yanyang] H;Hunan Agr Univ, Food Sci & Technol Coll, Changsha 410000, Hunan, Peoples R China.
关键词：
Fluorescent probe;Palladium detection;ESIPT;Phthalimide;Lysosome
摘要：
A novel lysosome-targetable phthalimide fluorescent probe was designed for detecting palladium based on ESIPT for signal transduction. The fluorescent probe conjugating with allylcarbamate displayed weak fluorescent due to the ESIPT process hinder by allylcarbamate. But with the addition of palladium, the ESIPT emission was recovery though the palladium-catalyzed deallylation reaction and the fluorescence intensity exhibited 40-fold enhancement at 511 nm. In addition, the probe showed excellent selectivity, high sensitivity, fast responds and low limit detection for palladium with a larger Stoke-shift. Moreover, the targetable probe was also successfully applied for detecting palladium in lysosomes of living cells. Hence, the probe though ESIPT modulation is a promising for monitoring palladium in practical samples. (C) 2018 Published by Elsevier B.V.
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英文
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Quantifying the Degree of Aggregation from Fluorescent Dye-Conjugated DNA Probe by Single Molecule Photobleaching Technology for the Ultrasensitive Detection of Adenosine
作者：
Shi, Xingbo* ;He, Yu;Gao, Wenli;Liu, Xiaoying;Ye, Zhongju;...
期刊：
Analytical Chemistry ,2018年90(6):3661-3665 ISSN：0003-2700
通讯作者：
Shi, Xingbo;Xiao, Lehui
作者机构：
[Shi, Xingbo; Gao, Wenli; He, Yu] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Shi, Xingbo] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Liu, Hua; Ye, Zhongju; Xiao, Lehui] Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.;[Liu, Xiaoying] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Shi, Xingbo] H;[Xiao, Lehui] N;Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.
摘要：
In this work, we demonstrated a single molecule photobleaching-based strategy for the ultrasensitive detection of adenosine. A modified split aptamer was designed to specifically recognize individual adenosine molecules in solution. The specific binding of dye-labeled short strand DNA probes onto the elongated aptamer strand in the presence of adenosine resulted in a concentration-dependent self-aggregation process. The degree-of-aggregation (DOA) of the short DNA probes on the elongated aptamer strand could then be accurately determined based on the single molecule photobleaching measurement. Through statistically analyzing the DOA under different target concentrations, a well-defined curvilinear relationship between the DOA and target molecule concentration (e.g., adenosine) was established. The limit-of-detection (LOD) is down to 44.5 pM, which is lower than those recently reported results with fluorescence-based analysis. Owing to the high sensitivity and excellent selectivity, the sensing strategy described herein would find broad applications in biomolecule analysis under complicated surroundings. © 2018 American Chemical Society.
语种：
英文
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近红外光谱无损检测在食用油脂分析中的应用研究进展
作者：
曾子琦;蒋立文;刘霞;李跑
期刊：
中国油脂 ,2018年43(8):137-142 ISSN：1003-7969
作者机构：
[曾子琦; 蒋立文; 刘霞; 李跑] 湖南农业大学食品科学技术学院, 食品科学与生物技术湖南省重点实验室, 长沙, 410128
关键词：
近红外光谱;食用油脂;种类鉴别;理化指标分析;掺伪掺假;含油量无损检测
摘要：
近红外光谱技术作为一种具有样品前处理简便、快速高效、可以实现多组分同时分析等优点的新型无损分析技术,在食用油脂分析领域扮演着重要角色。对近10年来近红外光谱技术在食用油脂无损检测中的应用进行了总结,概述了近红外光谱技术的分析类型,在食用油脂和食用油脂原料分析方面的应用。通过结合化学计量学方法,近红外光谱技术可以实现不同种类食用油脂的快速鉴别分析,食用油脂的各项理化指标（如游离脂肪酸的含量、碘值、酸值、过氧化值、皂化值等）的快速检测,食用油脂无损掺假检测,食用油脂原料（如玉米、棉籽、油菜籽等）含油量的无损分析。此外,针对当前食用成品油快速无损分析过程中的难题对未来该领域的研究方向进行了展望,以期近红外光谱技术在食用油脂无损分析领域得到更广泛的应用。
语种：
中文
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SPR在野菊花黄酮与淀粉相互作用的应用研究
作者：
佘银;李跑;高婉茹;黄昭;刘霞
期刊：
食品研究与开发 ,2018年39(7):23-28 ISSN：1005-6521
作者机构：
湖南农业大学食品科学技术学院食品科学与生物技术湖南省重点实验室,湖南长沙,410000;[李跑; 佘银; 高婉茹; 刘霞; 黄昭] 湖南农业大学
关键词：
野菊花黄酮;淀粉;表面等离子体共振;淀粉消化;抗氧化性
摘要：
This study evaluated the interaction of Chysanthremum indicum flavonoids(luteolin,acacetin and buddleoside)with starch by surface plasmon resonance(SPR).Their equilibrium dissociation constant(KD)were(4.822 4依0.06),(3.441 3依0.06)and(2.367 6依0.25)mmol/L,respectively.On this basis,it was found that three flavonoids had inhibitory effect on the digestion of starch.when the concentration was 100滋mol/L,the inhibition rate was 10.92%,16.02%and 26.71%,respectively.Moreover,the antioxidant activity of Chysanthremum indicum flavonoids was inhibited after the interaction with starch.The results showed that the number and position of hydroxyl groups played an important role in the interaction between the three flavonoids and starch.In addition,SPR can be used as a rapid method to study the interaction of flavonoids and starch,and provide useful information for the application of Chrysanthemum indicum flavonoids in food and pharmaceutical area.
语种：
中文
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乳酸菌粘附特性的研究新进展
作者：
佘银;罗芳;高婉茹;李鑫;刘霞
期刊：
食品研究与开发 ,2018年39(4):218-224 ISSN：1005-6521
作者机构：
湖南农业大学食品科学技术学院,湖南长沙,410000;[罗芳; 佘银; 高婉茹; 刘霞; 李鑫] 湖南农业大学
关键词：
乳酸菌;粘附研究方法;粘附作用;粘附素;粘附受体
摘要：
近年来, 乳酸菌以其益生功能成为研究的热点, 而乳酸菌的粘附特性是评价乳酸菌益生功能的重要指标之一. 目前, 有关研究乳酸菌粘附的新方法不断涌现, 如表面等离子体共振技术、 计算机分子模拟法等. 乳酸菌粘附素与胃肠道粘液受体相互作用的机理是阐明其粘附特性的关键.同时, 乳酸菌的理化性质 以及外部环境也会影响其粘附能力, 如菌体生长阶段、 菌液浓度、 pH 值、 蛋白酶等.对目前国内外有关乳酸菌粘附特性的研究方法、 粘附机理及其影响因素的研究进展进行综述, 并对其研究的发展方向进行了展望.
语种：
中文
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Three Citrus flavonoids retard the digestion of starch and its working mechanisms
作者：
Luo, Fang;Liu, Xia* ;She, Yin;Gao, Wanru
期刊：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ,2018年53(2):365-371 ISSN：0950-5423
通讯作者：
Liu, Xia
作者机构：
[She, Yin; Luo, Fang; Gao, Wanru; Liu, Xia] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Amylases;Antioxidants;Flavonoids;Plasmons;Scanning electron microscopy;Surface plasmon resonance;Alpha amylase;Anti-oxidant activities;Inhibitory kinetics;Naringin;Radical assay;Starch digestions;Surface plasmon resonance biosensor;Working mechanisms;Starch
摘要：
In this work, the effects of three Citrus flavonoids (naringin, neohesperidin and hesperidin) on α-amylase catalysed starch digestion were investigated. An inhibitory kinetics experiment demonstrated that they noncompetitively inhibit the activity of α-amylase with Ki values of, respectively, 25.21μm, 40.26μm, 40.69μm. Surface plasmon resonance assay showed three Citrus flavonoids can bind with starch with KD values of, respectively, 3.98±0.20mm, 4.92±0.15mm and 5.07±0.17mm. Scanning electron microscopy indicated they can delay the retrogradation of starch. The results of 1, 1-diphenyl-2-picryl hydrazyl radical assay indicate that the antioxidant activities of three Citrus flavonoids are significantly decreased by interacting with starch. These results illustrated three Citrus flavonoids that retard the digestion of starch not only through interaction with α-amylase, but also through interaction with starch. © 2017 Institute of Food Science and Technology
语种：
英文
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离子阱质谱测定脂质鉴别深海鱼油
作者：
李鑫;刘霞;李培武;王秀嫔
期刊：
食品科学 ,2018年39(18):199-203 ISSN：1002-6630
作者机构：
湖南农业大学食品科学技术学院, 食品科学与生物技术湖南省重点实验室, 湖南, 长沙, 410128;中国农业科学院油料作物研究所, 农业部油料作物生物学与遗传改良重点实验室;[李鑫; 李培武; 王秀嫔] 农业部油料及制品质量监督检验测试中心;[李鑫; 李培武; 王秀嫔] 农业农村部油料产品质量安全风险评估实验室(武汉);[李鑫; 李培武; 王秀嫔] 农业部生物毒素检测重点实验室, 湖北, 武汉, 430062
关键词：
深海鱼油;快速鉴别;离子阱质谱;脂质;二十二碳六烯酸;二十碳五烯酸
摘要：
建立基于高效液相色谱-离子阱质谱脂质识别技术,用于鉴别合成乙酯型和天然甘油酯型深海鱼油。使用反相液相色谱,C_(18)色谱柱,异丙醇和乙腈梯度洗脱分离脂质。使用离子阱质谱数据依赖型二级扫描获得脂质多级碎裂指纹信息。通过总离子流、一级质谱、二级质谱离子树识别脂质指纹信息。识别获知二十二碳六烯酸(docose hexaenoie acid,DHA)和二十碳五烯酸(eicosapentaenoic acid,EPA)在乙酯型深海鱼油中存在方式为CH_3CH_2ODHA和CH_3CH_2O-EPA,而在天然甘油酯型深海鱼油的存在方式为甘油三酯指纹信息。此方法能准确区分乙酯型深海鱼油和甘油酯型深海鱼油。
语种：
中文
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黄酮类化合物与食品成分相互作用研究进展
作者：
刘霞;罗芳;佘银;李鑫;高婉茹
期刊：
食品与生物技术学报 ,2017年36(10):1009-1015 ISSN：1673-1689
作者机构：
[李鑫; 罗芳; 佘银; 刘霞; 高婉茹] Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China
关键词：
黄酮类化合物;相互作用;蛋白质;脂肪;碳水化合物
摘要：
黄酮类化合物具有多种生物活性,能与食品中的某些成分发生相互作用,影响食品的品质;反之食品成分也会对黄酮类化合物的生物活性产生影响,因此黄酮类化合物和食品成分如蛋白质、脂肪、碳水化合物的相互作用研究,受到国内外的广泛关注。作者综述了黄酮类化合物和食品中蛋白质、脂肪、碳水化合物相互作用的研究现状,对其发展方向进行了展望。
语种：
中文
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Investigation of the interaction for three Citrus flavonoids and α-amylase by surface plasmon resonance
作者：
Liu, Xia* ;Luo, Fang;Li, Pao;She, Yin;Gao, Wanru
期刊：
Food Research International ,2017年97:1-6 ISSN：0963-9969
通讯作者：
Liu, Xia
作者机构：
[She, Yin; Luo, Fang; Gao, Wanru; Liu, Xia; Li, Pao] Hunan Agr Univ, Hunan Prov Key Lab Food Sci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
*Acarbose (PubChem CID: 122172882);*Atrazine (PubChem CID: 2256);*Citrus flavonoids;*DPPH;*Hesperidin (PubChem CID: 10621);*Interaction;*Naringin (PubChem CID: 442428);*Neohesperidin (PubChem CID: 442439);*Surface plasmon resonance biosensor;*alpha-Amylase
摘要：
Flavonoids, a class of natural drugs with broad biological activity, exhibit inhibitory effect on α-amylase. Citrus peel is a good source of flavonoids. The real-time interactions between three Citrus flavonoids (naringin, neohesperidin, hesperidin) and α-amylase were investigated by surface plasmon resonance biosensor, and were compared with the α-amylase inhibitors, acarbose. These results showed the binding affinities of naringin, neohesperidin and hesperidin with α-amylase reach the highest at pH6 with KD values of 2.27±0.18mM, 3.09±0.20mM and 3.51±0.09mM, and can be reinforced with 0.2M NaCl and 0.1M CaCl2, respectively. The results of 1, 1-diphenyl-2-picryl hydrazyl radical assay indicate that the antioxidant activities of naringin, neohesperidin and hesperidin are significantly inhibited by interacting with α-amylase, and the inhibition percentage are 47.61±0.034%, 22.81±0.037% and 21.01±0.051%, respectively. Additionally, it is found that both the number and the position of hydroxyl group play an important role in the interaction of three Citrus flavonoids and α-amylase. These results provide useful information for rapid screening inhibitors of α-amylase from plant-based food. © 2017 Elsevier Ltd
语种：
英文
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A new electrochemical platform for ultrasensitive detection of atrazine based on modified self-ordered Nb2O5 nanotube arrays
作者：
Yang, Min* ;Zhao, Xiaobin;Zheng, Shu;Liu, Xia* ;Jin, Bowen;...
期刊：
Journal of Electroanalytical Chemistry ,2017年791:17-22 ISSN：1572-6657
通讯作者：
Yang, Min;Liu, Xia
作者机构：
[Jin, Bowen; Yang, Min; Gan, Yang; Zhao, Xiaobin; Li, Huang] Harbin Inst Technol, Sch Chem & Chem Engn, MIIT Key Lab Crit Mat Technol New Energy Convers, Harbin 150001, Peoples R China.;[Zheng, Shu; Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yang, Min; Liu, Xia] H;Harbin Inst Technol, Sch Chem & Chem Engn, MIIT Key Lab Crit Mat Technol New Energy Convers, Harbin 150001, Peoples R China.;Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Herbicides;Iron oxides;Magnetite;Monoclonal antibodies;Nanoparticles;Nanotubes;Niobium oxide;Solutions;Yarn;Anodizations;Electrochemical platforms;Electrochemical systems;Electrosensing platform;Fe3O4 nanoparticles;Linear detection ranges;Optimized conditions;Ultrasensitive detection;Electrochemical sensors
摘要：
In this work, one dimensional self-ordered Nb2O5 nanotube arrays were grown from Nb substrate by anodization method and were modified with optimum amounts of chitosan (CS) and carboxyl-Fe3O4 nanoparticles. These nanocomposites were characterized by SEM, XRD, and XPS and used as electrode materials for the direct detection of atrazine traces in aqueous solution. The test signals were obtained from the interaction between anti-atrazine monoclonal antibody and atrazine and recorded using via differential pulse voltammetric (DPV) method in electrochemical system. These modified one-dimensional Nb2O5 nanotube arrays showed the linear detection ranges of 0.04ng/mL–0.6ng/mL and a detection limit of 0.018ng/mL for atrazine under the optimized conditions. These above results indicate that these Nb2O5 nanotube arrays are promising candidates for electrochemical sensors. © 2017 Elsevier B.V.
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英文
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