作者机构：
[Xiao, Hong-Bo; Sui, Guo-Guang] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Agr Univ, Hunan Prov Univ Key Lab Agr Biochem & Biotransfor, Changsha, Hunan, Peoples R China.;[Lu, Xiang-Yang] Hunan Coinnovat Ctr Ultilizat Bot Funct Ingredien, Changsha, Hunan, Peoples R China.;[Sun, Zhi-Liang] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China.
关键词：
Kaempferol;Angiopoietin-like protein 2;Murine mastitis
摘要：
Background: Mastitis is inflammation of a breast (or udder). Angiopoietin-like protein 2 (ANGPTL2) has been found as a key inflammatory mediator in mastitis. Purpose of this research was to investigate the mechanisms about repressing effect of kaempferol on mastitis. Methods: Forty mice were randomly divided into 4 groups (n = 10): C57BL/6J control mice, untreated murine mastitis, 10 mg/kg kaempferol treated murine mastitis (ip), and 30 mg/kg kaempferol treated murine mastitis (ip). Primary cultured mouse mammary epithelial cells (MMEC) were indiscriminately divided into seven groups including control group, 10 mu mol/L vehicle of kaempferol group, 10 mu mol/L kaempferol treated group, 20 mu g/mL LPS treated group, 1 mmol/L kaempferol plus LPS treated group, 3 mu mol/L kaempferol plus LPS treated group, and 10 mu mol/L kaempferol plus LPS treated group. Results: In murine mastitis, kaempferol (10 or 30 mg/kg) treatment prevented mastitis development, decreased myeloperoxidase (MPO) production, interleukin (IL)-6 level, tumour necrosis factor-alpha (TNF-alpha) concentration, and ANGPTL2 expression. In MMEC, kaempferol (1, 3 or 10 mu M) reduced MPO production, TNF-alpha concentration, IL-6 level, and ANGPTL2 expression. Conclusions: The results in present study show that kaempferol modulates the expression of ANGPTL2 to lessen the mastitis in mice. (c) 2018 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.
摘要：
A newly emerging porcine circovirus, designated PCV3, has been reported in various countries (USA, Poland, South Korea and China) since 2017. Its presence may be associated with porcine dermatitis and nephropathy syndrome (PDNS), reproductive failure, and multisystem inflammation. In this study, we report identification of PCV3 in cases of reproductive failure in various regions in Hunan, China. From January 2015 to December 2016, sera were collected from 190 sows from seven farms with reproductive problems. Specifically, 85 samples were from sows with a history of reproductive failure, whereas the remaining 105 were from healthy sows. The PCV3-positive rate was significantly higher in sows with reproductive failure (45.9%) than in healthy sows (21.9%), based on quantitative PCR (qPCR) assays. Although phylogenetic analysis based on the cap gene suggested that these PCV3 isolates belonged to the clade PCV3a, amino acid sequence variations in the Cap protein still occurred among these isolates, and these might have contributed to antigenic alterations of the Cap protein, based on the Jameson-Wolf antigenic index. Finally, we concluded that PCV3 was circulating in sows in Hunan province, China. However, the association of PCV3 with reproductive failure in sows and its potential for vertical transmission need to be studied further.
摘要：
Nanotechnology holds the potential to revolutionize many important scientific fields, ranging from biology to medicine. The combination of nanotechology and molecular biology opens the door to many exciting possibilities for the detection and manipulation of molecules using nanodevices, holding high promise for both medical and biological research, as well as providing practical applications at the cellular level. NanoBiotechology is a new peer–reviewed scientific journal, to be published quarterly commencing in 2005, that provides a multidisciplinary forum for state–of–the–art nanobiotechnological methods, instrumentation, and research. AudienceScientists, Engineers, Academics, Teachers, and Students Aims and ScopeNanoBiotechnology is an international peer-reviewed journal that publishes research papers covering all aspects of the emerging and rapidly growing research area at the intersection of nanotechnology, molecular biology and biomedical sciences. NanoBiotechnology is intended to serve as an authoritative forum that presents in a timely manner the state–of–the–art, multidisciplinary research and technological advances in theory, instrumentation and methods as well as applications in various areas of nanotechnology related to biology and medicine. The convergence of nanotechnology and biomedical sciences opens the possibility for a wide variety of biological research topics and medical uses at the molecular and cellular level. The fundamental features of biological systems such as self–assembly, self–replication, and highly specific recognition open new routes to extend solid–state top–down fabrication nanotechnologies. The application of nanotechnology in biomedical sciences will provide information with unprecedented precision and sensitivity, which will not only provide much deeper understandingof biosystems but also lead to the development of new revolutionary modalities of biomolecular manufacturing, early diagnostics, medical treatment, and disease prevention beyond the cellular level to that of DNA and individual proteins, the building blocks of the life process. The long–term goal of NanoBiotechnology is to contribute to the information transfer and explosive growth of this important research area. Coverage in the Journals@Ovid database begins with the Vol. 1 No. 1 2005 issue.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
*Angiopoietin-like protein 4;*Forsythia suspense;*Obese C57BL/6J mice;*Peroxisome proliferator-activated receptor beta/delta;*Phillyrin
摘要：
Objective: Previous investigations have shown that the peroxisome proliferator activated receptor beta/delta (PPAR beta/delta)-angiopoietin-like protein 4 (ANGPTL4) pathways may be a new pharmacologic target for treatment of obesity. The present study was conducted to test the effect of phillyrin, a glucoside, on obesity in mice.& para;& para;Method: Fifty mice were randomly divided into 5 groups (n =10): control group (C57BL/6J mice), obese mice group, two groups of obese mice treated with phillyrin (15 or 45 mg/kg/day), one group of obese mice treated with PPAR beta/delta agonist GW0742 (3 mg/kg/day). Twelve weeks after treatment, body weight, liver weight, fat weight, lipid levels in the liver, serum levels of tumour necrosis factor-alpha (INF-alpha), leptin, and insulin, expression of PPAR beta/delta, ANGPTL4, and AMP-activated protein kinase (AMPK) were determined.& para;& para;Results: Treatment with phillyrin (15 or 45 mg/kg) significantly decreased body weight, liver weight, fat weight, hepatic total cholesterol, free fatty acid, and triglyceride concentrations, serum levels of TNF-alpha, leptin, and insulin concomitantly with up-regulated expression of PPAR beta/delta, ANGPTL4, and p-AMPK-alpha. In addition, GW0742 has similar effect of phillyrin.& para;& para;Conclusions: The present results suggest that phillyrin could regulate the PPAR beta/delta -ANGPTL 4 pathway to lower body weight in obese C57BL/6J mice. (C) 2017 Asia Oceania Association for the Study of Obesity. Published by Elsevier Ltd. All rights reserved.
作者机构：
[Liu, Guo-Hua; Zhu, Xing-Quan] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Zhang, Long-Xian] Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China.;[Zou, Feng-Cai] Yunnan Agr Univ, Coll Vet Med, Kunming 650201, Yunnan, Peoples R China.;[Yuan, Zi-Guo] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.;[Zhao, Guang-Hui] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China.
通讯机构：
[Zhu, Xing-Quan; Hu, Min] H;[Suo, Xun; Zhu, Xing-Quan] C;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China.;China Agr Univ, Natl Anim Protozoa Lab, Beijing 100193, Peoples R China.
关键词：
China;Teaching;Veterinary parasitology;Veterinary science and medicine
摘要：
China has made significant achievements in social-economic development in the last three decades, and the numbers of livestock and companion animals are rapidly increasing. Some advances have been made in the control and prevention of animal parasitic diseases, but there are still some significant challenges, particularly in relation to foodborne parasitic zoonoses and vector-borne diseases. In addition, new molecular (e.g., genomic and transcriptomic) technologies have been developed and are gradually being introduced into the veterinary parasitology field. Therefore, teaching of veterinary parasitology in Chinese universities has undergone significant changes over the years, in terms of topics, depth and breadth, and also in the ways in which courses are delivered. In this article, we describe the current status of veterinary parasitology teaching at the undergraduate and postgraduate levels in Chinese universities, summarise changes and improvement in veterinary parasitology teaching, and discuss the challenges and opportunities for veterinary parasitology teaching in the 21st century, including the use of new teaching technologies and the integration of the “One Health” concept into veterinary parasitology courses.
摘要：
Marshallagia marshalli (Nematoda: Trichostrongylidae) infection can lead to serious parasitic gastroenteritis in sheep, goat, and wild ruminant, causing significant socioeconomic losses worldwide. Up to now, the study concerning the molecular biology of M. marshalli is limited. Herein, we sequenced the complete mitochondrial (mt) genome of M. marshalli and examined its phylogenetic relationship with selected members of the superfamily Trichostrongyloidea using Bayesian inference (BI) based on concatenated mt amino acid sequence datasets. The complete mt genome sequence of M. marshalli is 13,891 bp, including 12 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes. All protein-coding genes are transcribed in the same direction. Phylogenetic analyses based on concatenated amino acid sequences of the 12 protein-coding genes supported the monophylies of the families Haemonchidae, Molineidae, and Dictyocaulidae with strong statistical support, but rejected the monophyly of the family Trichostrongylidae. The determination of the complete mt genome sequence of M. marshalli provides novel genetic markers for studying the systematics, population genetics, and molecular epidemiology of M. marshalli and its congeners.
摘要：
In the genus Macleaya, Macleaya cordata and Macleaya microcarpa have been recognized as traditional herbs that are primarily distributed in China, North America, and Europe and have a long history of medicinal usage. These herbs have been long valued and studied for detumescence, detoxification, and insecticidal effect. This review aims to provide comprehensive information on botanical, phytochemical, pharmacological, and toxicological studies on plants in the genus Macleaya. Plants from the genus of Macleaya provide a source of bioactive compounds, primarily alkaloids, with remarkable diversity and complex architectures, thereby having attracted attention from researchers. To date, 291 constituents have been identified and/or isolated from this group. These purified compounds and/or crude extract possess antitumor, anti-inflammatory, insecticidal, and antibacterial activities in addition to certain potential toxicities. Macleaya species hold potential for medicinal applications. However, despite the pharmacological studies on these plants, the mechanisms underlying the biological activities of active ingredients derived from Macleaya have not been thoroughly elucidated to date. Additionally, there is a need for research focusing on in vivo medical effects of Macleaya compounds and, eventually, for clinical trials.
摘要：
The sheep ked Melophagus ovinus is mainly found in Europe, Northwestern Africa, and Asia. Although M. ovinus is an important ectoparasite of sheep in many countries, the population genetics, molecular biology, and systematics of this ectoparasite remain poorly understood. Herein, we determined the mitochondrial (mt) genome of M. ovinus from Gansu Province, China (MOG) and compared with that of M. ovinus Xinjiang Uygur Autonomous Region, China (MOX). The mt genome sequence (15,044 bp) of M. ovinus MOG was significantly shorter (529 bp) than M. ovinus MOX. Nucleotide sequence difference in the whole mt genome except for non-coding region was 0.37% between M. ovinus MOG and MOX. For the 13 protein-coding genes, comparison revealed sequence divergences at both the nucleotide (0–1.1%) and amino acid (0–0.59%) levels between M. ovinus MOG and MOX, respectively. Interestingly, the cox1 gene of M. ovinus MOX is predicted to employ unusual mt start codons AAA, which has not been predicted previously for any parasite genome. Phylogenetic analyses showed that M. ovinus (Hippoboscoidea) is related to the superfamilies Oestroidea + Muscoidea. Our results have also indicated the paraphylies of the four families (Anthomyiidae, Calliphoridae, Muscidae, and Oestridae) and two superfamilies (Oestroidea and Muscoidea). This mt genome of M. ovinus provides useful molecular markers for studies into the population genetics, molecular biology, and systematics of this ectoparasite.
摘要：
Ticks are obligate blood-sucking ectoparasites that infect a wide range of animals and humans, causing a variety of both human and animal diseases around the world. Ixodes nipponensis is the most commonly reported tick in Korea and Japan, but it is very rare in China. In this study, six I. nipponensis samples were collected from three black goats in Hunan province, China. Ticks identified morphologically as I. nipponensis were then examined by PCR with two different molecular markers: mitochondrial cox1 and the second internal transcribed spacer of ribosomal DNA genes. Sequence comparison and phylogenetic analysis of the cox1 sequences confirmed that all of the examined hard Ixodes ticks represented I. nipponensis. This finding indicates a potential risk of zoonotic I. nipponensis infection in humans and animals in China. To our knowledge, this is the first report documenting the occurrence of I. nipponensis infection in goats in China.
