Profiling the chlorogenic acids of aster by HPLC-MSn
作者：
Clifford, Michael N.* ;Zheng, Wang;Kuhnert, Nikolai
期刊：
Phytochemical Analysis ,2006年17(6):384-393 ISSN：0958-0344
通讯作者：
Clifford, Michael N.
作者机构：
[Clifford, Michael N.] Univ Surrey, Sch Biomed & Mol Sci, Ctr Nutr & Food Safety, Surrey GU2 7XH, England.;Hunan Agr Univ, Coll Biosci & Bioengn, Changsha 410128, Hunan, Peoples R China.;Univ Surrey, Sch Biomed & Mol Sci, Surrey GU2 7XH, England.
通讯机构：
[Clifford, Michael N.] U;Univ Surrey, Sch Biomed & Mol Sci, Ctr Nutr & Food Safety, Surrey GU2 7XH, England.
关键词：
Aster;Asteraceae;caffeoylquinic acids;caffeoyl-feruloylquinic acids;chlorogenic acids;p-coumaroyl-caffeoylquinic acids;p-coumaroyl-feruloylquinic acids;p-coumaroylquinic acids;dicaffeoylquinic acids;diferuloylquinic acids;feruloylquinic acids;HPLC-MSn
摘要：
Using HPLC-MSn, 33 chlorogenic acids were identified in an aqueous-alcoholic extract of Aster ageratoides Turcz. flower buds. These were three isomers each of p-coumaroylquinic acid, caffeoylquinic acid, feruloylquinic acid, dicaffeoylquinic acid and diferuloylquinic acid, and six isomers each of p-coumaroyl-caffeoylquinic acid, p-coumaroyl-feruloylquinic acid and caffeoyl-feruloylquinic acid. Only the caffeoylquinic acids and dicaffeoylquinic acids have been reported previously in Asteraceae. Three of the six p-coumaroyl-feruloylquinic acids (3-feruloyl-4-p-coumaroylquinic acid, ′3-feruloyl-5-p-coumaroylquinic acid and 4- feruloyl-5-p-coumaroylquinic acid) have not been observed previously in nature. Cis-5-p-coumaroylquinic acid was identified at a concentration ca 25% that of the more common trans isomer. The feruloylquinic acids and diferuloylquinic acids dominated the mono- and di-acyl chlorogenic acid fractions, respectively, making this plant material a useful source of these commercially non-available substances. These 33 chlorogenic acids were not detected in the leaves or stem of A. ageratoides Turcz., or in the flower buds of A. ageratoides Turcz. var. Gerla or A. kalimeris indica (L) Sch. Bip. Only the feruloylquinic acids were detected in the root of A. ageratoides Turcz. It was not possible to detect any 1-acyl chlorogenic acids, any chlorogenic acids with a succinic acid substituent, or any chlorogenic acids based on muco-quinic acid. Copyright © 2006 John Wiley & Sons, Ltd.
语种：
英文
展开
Response of weeping willows to linear alkylbenzene sulfonate
作者：
Yu, Xiaozhang* ;Trapp, Stefan;Zhou, Puhua;Peng, Xiaoying;Cao, Xi
期刊：
Chemosphere ,2006年64(1):43-48 ISSN：0045-6535
通讯作者：
Yu, Xiaozhang
作者机构：
[Yu, Xiaozhang] Hunan Agr Univ, Dept Environm Sci, Changsha 410128, Hunan, Peoples R China.;Tech Univ Denmark, Inst Environm & Resources DTU, DK-2800 Lyngby, Denmark.;Hunan Agr Univ, Dept Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Yu, Xiaozhang] H;Hunan Agr Univ, Dept Environm Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
LAS;linear alkylbenzene sulfonate;toxicity;transpiration;willows;enzyme activity;SLUDGE-AMENDED SOILS;RISK-ASSESSMENT;AGRICULTURAL SOIL;TERRESTRIAL ENVIRONMENT;SUPEROXIDE DISMUTASES;PLANTS
摘要：
Linear alkylbenzene sulfonate (LAS) is the most commonly used anionic surfactant in laundry detergents and cleaning agents. LAS compounds are found in surface waters and soils. The short-term acute toxicity of LAS to weeping willows (Salix babylonica L.) was investigated. Willow cuttings were grown in hydroponic solution spiked with LAS at 24.0 ± 1 °C for 192 h. The normalized relative transpiration of plants was used to determine toxicity. Severe reduction of the transpiration was only found for high doses of LAS ({greater than or slanted equal to}240 mg l-1). Chlorophyll contents in leaves of treated plants varied with the dose of LAS, but there was no significant linear correlation. The activities of the enzymes superoxide dismutases (SOD), catalase (CAT), and peroxidase (POD) were quantified at the end of experiments. At higher concentrations of LAS ({greater than or slanted equal to}240 mg l-1), the activities of SOD and CAT were decreased. The correlation between the dose of LAS and the POD activity in leaf cells was the highest of all enzyme assays (R2 = 0.5). EC50 values for a 50% inhibition of the transpiration of the trees were estimated to 374 mg l-1 (72 h) and 166 mg l-1 (192 h). Results from this experiment indicated that phytotoxic effects of LAS on willow trees are not expected for normal environmental conditions. © 2005 Elsevier Ltd. All rights reserved.
语种：
英文
展开
覆盖与间作对亚热带丘陵茶园地温时空变化的影响
作者：
彭晚霞;宋同清;肖润林;杨知建;王久荣;...
期刊：
应用生态学报 ,2006年17(5):778-782 ISSN：1001-9332
通讯作者：
Peng, W.
作者机构：
[肖润林; 王久荣; 宋同清] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;[杨知建] College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China;[李盛华] Xiangfeng Tea Factory of Changsha County, Changsha 410158, China;[夏艳珺; 彭晚霞] Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China, College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Peng, W.] I;Institute of Subtropical Agriculture, , Changsha 410125, China
关键词：
茶园地温;稻草覆盖;白三叶草间作;时空变化
摘要：
研究了亚热带丘陵茶园连续4年进行稻草覆盖与白三叶草间作对茶园地温时空动态变化的影响．结果表明，茶园地温具有明显的时空动态变化特征和位相滞后现象，与茶树的年生长发育周期各阶段的最适温度非常吻合．稻草覆盖与白三叶草间作改变了土壤热量交换层（地表层）的性质，具有升温时降温和降温时增温、保温的双向动态调控作用，降低了日较差，增强了同一土层温度的稳定性，其调控效果为随着土壤深度增加而降低，13：00〉19：00〉7：00，降温大于增温和保温，降温幅度随气温的升高而增大，增温和保温随气温的下降而加强，间作白三叶草的降温效果大于稻草覆盖，保温效果则相反．茶园地温时空变化转换点得到调节，显著地降低了有害高温的出现次数，明显地降低了持续高温期的土壤温度，有效地缩短了极限高温时间．
语种：
中文
展开
符合经济生态效益的农田化肥施用量
作者：
向平安;周燕;郑华;燕惠民;黄璜;...
期刊：
应用生态学报 ,2006年17(11):2059-2063 ISSN：1001-9332
通讯作者：
Xiang, P.
作者机构：
[向平安; 黄璜; 黄清云] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[周燕] Technical Center of Changsha Cigarette Factory, Changsha 410014, China;[郑华] Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;[燕惠民] Agro-Environment Protection and Administration Station of Hunan Province, Changsha 410011, China
通讯机构：
[Xiang, P.] C;College of Bioscience and Biotechnology, , Changsha 410128, China
关键词：
化肥;粮食生产;经济;生态;洞庭湖区
摘要：
在洞庭湖区研究了农田化肥的外部成本和经济生态施用量.采用环境影响经济评价方法,估算了化肥的外部成本;运用外部不经济性原理和生产函数模型研究了兼顾农户经济效益、生态效益的农田适宜施肥量.结果表明,2002年洞庭湖区粮食生产中因化肥施用不当而带来的外部成本约为1.35*10~8元,每千克纯氮的外部成本约为0.3元.在洞庭湖区目前生产条件下,兼顾生产、经济、生态三效益的适宜施肥量是208.26～210.65 kg·hm~(-2),相应的粮食产量为5528～5539 kg·hm~(-2).2002年洞庭湖区粮食生产实际施肥量已超过农户经济最优施肥量和生态适宜施肥量,建议政府在一些化肥高投入、高污染的地区征收环境污染税.
语种：
中文
展开
铅胁迫对黄瓜幼苗抗氧化酶活性及同工酶的影响
作者：
刘素纯;萧浪涛;廖柏寒;鲁旭东;匡逢春;...
期刊：
应用生态学报 ,2006年17(2):300-304 ISSN：1001-9332
通讯作者：
Liu, S.
作者机构：
[刘素纯; 童建华; 廖柏寒; 匡逢春; 萧浪涛; 赵文魁] Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China;College of Life Sciences, Xiaogan University, Xiaogan 432000, China;[鲁旭东] Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China, College of Life Sciences, Xiaogan University, Xiaogan 432000, China
通讯机构：
[Liu, S.] H;Hunan Provincial Key Laboratory of Phytohormones and Growth Development, , Changsha 410128, China
关键词：
铅胁迫;黄瓜幼苗;同工酶;过氧化物酶;超氧化物歧化酶;过氧化氢酶
摘要：
采用水培法和聚丙烯酰胺凝胶电泳法，研究铅胁迫对黄瓜幼苗过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性及同工酶的影响．结果表明，铅胁迫下黄瓜幼苗地上部POD活性除第5天外均随铅浓度的增加而逐渐降低，POD同工酶谱带和表达量减少．在0～500mg·L^-1铅浓度范围内，SOD活性随铅浓度的增加而增加，第7天达到最大值后急剧下降，低于同期对照值。900mg·L^-1铅处理SOD活性随时间的延长逐步降低，SOD酶谱带和表达量与铅浓度呈负相关．CAT酶谱带无明显变化，而表达量存在差异．
语种：
中文
展开
日本血吸虫SjIR3 DNA疫苗诱导的抗攻击感染保护力
作者：
郝知友;黄复深;袁鑫;陈尔曼;邱元;...
期刊：
中国寄生虫学与寄生虫病杂志 ,2006年24(6):445-448 ISSN：1000-7423
通讯作者：
Hao, Z.Y.
作者机构：
[郝知友; 黄复深; 袁鑫; 陈尔曼; 邱元; 刘毅] 湖南农业大学动物医学院;湖南农业大学细胞工程重点实验室 长沙410128
关键词：
日本血吸虫;核酸疫苗;小鼠;免疫保护力
摘要：
目的 探讨日本血吸虫SjIR3核酸疫苗诱导小鼠抗日本血吸虫攻击感染的保护效果.方法 用SjIR3的特异引物构建疫苗SjIR3/pC.54只昆明小鼠随机分为3组:A组(对照组)、B组(空质粒组)和C组(实验组)分别肌注生理盐水(100 μl)、pcDNA3.0和SjIR3/pC(各50 μg),共免疫3次,每次间隔2周.于每次免疫前和感染前尾静脉采血,ELISA检测血清IgG抗体水平.末次免疫后2周,各组剖杀其中6只小鼠取脾细胞,分别用ConA或rSjIR3重组蛋白诱导,用四甲基偶氮唑盐微量酶反应比色法(MTT)检测脾淋巴细胞增殖情况.各组余下小鼠分别经腹部感染日本血吸虫尾蚴40±1条,45 d后宰杀,观察实验组小鼠的减虫率和肝减卵率.结果 ELISA结果显示,A组与B组IgG抗体水平变化不明显(P＞0.05),C组于末次免疫后2周(攻击感染前)IgG抗体水平最高为0.78±0.05,且与A、B两组比较差异有统计学意义(P＜0.01);末次免疫后,实验组小鼠脾淋巴细胞分别在ConA或rSjIR3重组蛋白诱导下可增殖,分别为0.57±0.02、0.68±0.01,与A、B组相比差异有统计学意义(P＜0.01).经SjIR3/pC免疫的实验组小鼠攻击感染后可产生29.4%的减虫率和36.6%的肝减卵率.结论 SjIR3/pC核酸疫苗具有较好的免疫原性,可诱导小鼠产生部分免疫保护力.
语种：
中文
展开
Mutation of the RESURRECTION1 Locus of Arabidopsis Reveals an Association of Cuticular Wax with Embryo Development
作者：
Chen, XB;Goodwin, SM;Liu, XL;Chen, XL;Bressan, RA;...
期刊：
PLANT PHYSIOLOGY ,2005年139(2):909-919 ISSN：0032-0889
通讯作者：
Jenks, MA
作者机构：
[Jenks, MA] Hunan Agr Univ, Crop Gene Engn Key Lab Human Province, Changsha 410128, Peoples R China.;Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA.
通讯机构：
[Jenks, MA] H;Hunan Agr Univ, Crop Gene Engn Key Lab Human Province, Changsha 410128, Peoples R China.
摘要：
Insertional mutagenesis of Arabidopsis (Arabidopsis thaliana) was used to identify a novel recessive mutant, designated resurrection1 (rst1), which possesses a dramatic alteration in its cuticular waxes and produces shrunken nonviable seeds due to arrested embryo development. The RST1 gene sequence associated with these phenotypes was verified by three independent, allelic, insertion mutants, designated rst1-1, rst1-2, and rst1-3, with inserts in the first exon, 12th intron, and fourth exon, respectively. These three rst1 allelic mutants have nearly identical alterations in their wax profiles and embryo development Compared to wild type, the wax on rst1 inflorescence stems is reduced nearly 60% in total amount, has a proportional reduction in aldehydes and aldehyde metabolites, and has a proportional increase in acids, primary alcohols, and esters. Compared to wild type, the C29 alkanes on rst1 are nearly 6-fold lower, and the C30 primary alcohols are 4-fold higher. These results indicate that rst1 causes shunting of most wax precursors away from alkane synthesis and into the primary-alcohol-producing branch of the pathway. In contrast to stems, the wax on rst1 mutant leaves increased roughly 43% in amount relative to the wild type, with the major increase occurring in the C31 and C33 alkanes. Unique among known wax mutants, approximately 70% of rst1 seeds are shrunken and nonviable, with these being randomly distributed within both inflorescence and silique. Viable seeds of rst1 are slightly larger than those of wild type, and although the viable rst1 seeds contain more total triacylglycerol-derived fatty acids, the proportions of these fatty acids are not significantly different from wild type. Shrunken seeds contain 34% of the fatty acids of wild-type seeds, with proportionally more palmitic, stearic, and oleic acids, and less of the longer and more desaturated homologs. Histological analysis of aborted rst1 seeds revealed that embryo development terminates at the approximate heart-shaped stage, whereas viable rst1 and wild-type embryos develop similarly. The RST1 gene encodes a predicted 1,841-amino acid novel protein with a molecular mass of 203.6 kD and a theoretical pI of 6.21. The RST1 transcript was found in all tissues examined including leaves, flowers, roots, stems, and siliques, but accumulation levels were not correlated with the degree to which different organs appeared affected by the mutation. The new RST1 gene reveals a novel genetic connection between lipid synthesis and embryo development; however, RST1's exact role is still quite unknown. The degree to which RST1 is associated with lipid signaling in development is an important focus of ongoing studies. © 2005 American Society of Plant Biologists.
语种：
英文
展开
Immunosensor for rapid detection of gibberellin acid in the rice grain
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2005年53(5):1348-1353 ISSN：0021-8561
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Dept Environm Sci & Engn, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Prov, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Dept Environm Sci & Engn, Chem & Chem Engn Coll, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
immunosensor;gibberellin acid;competitive immunoreaction;copper ion labeled
摘要：
A rapid, selective, sensitive, accurate, and inexpensive immunosensor for gibberellin acid detection was designed by coupling immunoassay with the square wave anodic stripping voltammetry (SWASV) technique involving copper ion labeled antigen in the competitive immunoreaction. The response signal expressed as the percentage of current reduction CR % (y) is linearly related to the concentration of GA (x) in the 1 μg/mL to ∼150 μg/mL range with a regression equation of the form y = 0.44x + 15.59 and a correlation coefficient of 0.99. The results of the immunosensor assay were compared with those obtained by HPLC and ELISA, which show a satisfactory agreement. The immunosensor was used to determine the GA content in the hybrid rice grain samples taken in the growing period. © 2005 American Chemical Society.
语种：
英文
展开
伏令夏橙愈伤组织体细胞胚发生中多胺水平的变化
作者：
刘华英;萧浪涛;鲁旭东;胡家金;吴顺;...
期刊：
植物生理与分子生物学学报 ,2005年31(3):275-280 ISSN：1671-3877
作者机构：
[刘华英] Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China
关键词：
多胺;体细胞胚发生;伏令夏橙
摘要：
以继代培养8年的伏令夏橙愈伤组织为材料,研究了不同类型愈伤组织体细胞胚发生能力的差异和多胺水平的变化及两者之间的关系.结果表明,胚性愈伤组织的多胺含量高于非胚性愈伤组织,体细胞胚发生能力与多胺水平呈正相关.体细胞胚发生早期Put含量的增加有利于体细胞胚发生.球形胚大量形成时,Spd达到最高值;球形胚发育后期并有少量心形胚形成时Spm达到峰值.随着倍性的增加,伏令夏橙体细胞胚发生能力降低.精氨酸脱羧酶的活性变化与Put水平呈正相关,表明它是调节伏令夏橙体细胞胚发生中多胺水平的重要因子.
语种：
中文
展开
Optimal sampling of a population to determine QTL location, variance, and allelic number
作者：
Wu, XL;Jannink, JL*
期刊：
Theoretical and Applied Genetics ,2004年108(7):1434-1442 ISSN：0040-5752
通讯作者：
Jannink, JL
作者机构：
[Jannink, JL] Iowa State Univ Sci & Technol, Dept Agron, Ames, IA 50011 USA.;Hunan Agr Univ, Ctr Life Sci Res, Hunan 410128, Peoples R China.
通讯机构：
[Jannink, JL] I;Iowa State Univ Sci & Technol, Dept Agron, Ames, IA 50011 USA.
摘要：
In a population intended for breeding and selection, questions of interest relative to a specific segregating QTL are the variance it generates in the population, and the number and effects of its alleles. One approach to address these questions is to extract several inbreds from the population and use them to generate multiple mapping families. Given random sampling of parents, sampling strategy may be an important factor determining the power of the analysis and its accuracy in estimating QTL variance and allelic number. We describe appropriate multiple-family QTL mapping methodology and apply it to simulated data sets to determine optimal sampling strategies in terms of family number versus family size. Genomes were simulated with seven chromosomes, on which 107 markers and six QTL were distributed. The total heritability was 0.60. Two to ten alleles were segregating at each QTL. Sampling strategies ranged from sampling two inbreds and generating a single family of 600 progeny to sampling 40 inbreds and generating 40 families of 15 progeny each. Strategies involving only one to five families were subject to variation due to the sampling of inbred parents. For QTL where more than two alleles were segregating, these strategies did not sample QTL alleles representative of the original population. Conversely, strategies involving 30 or more parents were subject to variation due to sampling of QTL genotypes within the small families obtained. Given these constraints, greatest QTL detection power was obtained for strategies involving five to ten mapping families. The most accurate estimation of the variance generated by the QTL, however, was obtained with strategies involving 20 or more families. Finally, strategies with an intermediate number of families best estimated the number of QTL alleles. We conclude that no overall optimal sampling strategy exists but that the strategy adopted must depend on the objective.
语种：
英文
展开
长期酒精摄入对大鼠糖酵解途径关键酶活性的影响
作者：
庞红;张军;毛丽梅;杨年红;应年红;...
期刊：
卫生研究 ,2004年33(2):195-197 ISSN：1000-8020
通讯作者：
Pang, H.
作者机构：
[彭国平; 范适; 饶力群] 湖南农业大学生物科学技术学院;[陈平] 湖南农业大学东方科技学院;[向华] 湖南省医药学校
关键词：
酒精;糖酵解途径;葡萄糖激酶;磷酸果糖激酶;己糖激酶
摘要：
目的 研究长期酒精摄入对大鼠糖酵解途径及其关键酶活性的影响。方法 70只Wistar大鼠 (雌雄各半 )随机分为 5组 ,分别给予蒸馏水或不同浓度的酒精溶液 (10 %、30 %、5 0 % ,V V)灌胃。 9周后 ,取肝脏测定糖酵解途径关键酶 -葡萄糖激酶 (GK)、磷酸果糖激酶 (PFK)和己糖激酶 (HK)的活性。结果 (1) 30 %、5 0 %酒精组大鼠GK和PEK活性与对照组相比降低 (P <0 0 5 ) ;(2 )各组大鼠HK活性差异均没有显著性 ;(3)GK、PFK活性与酒精剂量显著相关 (r =- 0 99～ - 0 97,P <0 0 5 )。结论 长期酒精摄入可使GK和PFK)活性降低 ,从而抑制糖酵解过程。这也可能是酒精升高血糖 ,影响血糖调节机制进而导致糖尿病的机制之一
语种：
中文
展开
气象因子和矿质元素对虎杖根茎白藜芦醇含量的影响
作者：
曹庸;张敏;于华忠;李国章;杜亚填;...
期刊：
应用生态学报 ,2004年15(7):1143-1147 ISSN：1001-9332
通讯作者：
Cao, Y.
作者机构：
[萧浪涛] Hunan Prov. Key Lab. of Phytohorms, Hunan Agricultural University, Changsha 410128, China;[张敏; 于华忠; 李国章; 杜亚填] Key Lab.Hunan.For.Prod./Chem.Indust., Jishou University, Zhangjiajie 427000, China;[曹庸] Hunan Prov. Key Lab. of Phytohorms, Hunan Agricultural University, Changsha 410128, China, Key Lab.Hunan.For.Prod./Chem.Indust., Jishou University, Zhangjiajie 427000, China
通讯机构：
[Cao, Y.] H;Hunan Prov. Key Lab. of Phytohorms, , Changsha 410128, China
关键词：
虎杖;白藜芦醇;矿质营养;气象因子;主成分分析
摘要：
对全年各月虎杖材料中白藜芦醇含量,及其Ca、Cu、Fe、K、Mg、Na、Zn 8种矿质元素积累进行检测,结合气象因子动态变化,采用主成分分析法,研究了不同季节虎杖白藜芦醇含量动态变化与其矿质元素、气象因子动态变化的关系.结果表明,Cu与Fe,Mg与K、Zn,Ca与月日照时数,K与Zn相关系数分别为0.812、0.871、0.793、0.602、0.729,呈极显著正相关;Cu、Mn与月平均气温,Fe与K相关系数分别为-0.738、-0.712、-0.766,呈极显著负相关;Cu与白藜芦醇含量呈显著负相关.找出了影响虎杖白藜芦醇含量动态变化的5个主成分,其中"酶促反应促进性矿质营养因子","高温、强照射气象因子"的贡献率分别为35.539%、33.358%,合计为68.897%,二者对不同季节虎杖白藜芦醇的变异贡献率最大.同时进行白藜芦醇含量的动态变化与主成分的多元相关、回归分析,建立了回归方程.
语种：
中文
展开
Amperometric immunosensor based on polypyrrole/poly(m-pheylenediamine) multilayer on glassy carbon electrode for cytokinin N6-(Δ2-isopentenyl) adenosine assay
作者：
Li, J;Xiao, LT;Zeng, GM;Huang, GH;Shen, GL* ;...
期刊：
Analytical Biochemistry ,2003年321(1):89-95 ISSN：0003-2697
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Province, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chem Biosensing & Chemometr, Changsha 410082, Peoples R China.
关键词：
immunosensor;N-6-(Delta(2)-isopentenyl) adenosine (iPA);bienzyme catalysis;competitive immunoreaction
摘要：
A novel immunosensor based on a multilayer-coated glassy carbon electrode was designed to determine isopentenyl adenosine (iPA) in plants. The multilayer consists of polypyrrole and poly(m-phenylenediamine) with K 4Fe(CN)6 and horseradish peroxidase (HRP) entrapped during electropolymerization. The ferrocyanide doped in polypyrrole functions as the mediator. The glucose oxidase bound on the immunosensor by the competitive immunoreaction involving iPA catalyzed the oxidation of the added glucose with the formation of H2O2, which is in turn reduced in the presence of HRP entrapped in poly(m-phenylenediamine). The current of the oxidized production of ferrocyanide reduced at -50mV is inversely proportional to the concentration of iPA in the competitive immunoreaction. This immunosensor is able to be used about 40 times; after that its surface can be regenerated for a new immunosensor assembly by washing with 0.1M citrate-phosphate buffer (pH 4.6). The percentage of current response reduction (CR%) (y) is linearly related to the logarithm of the concentration of iPA (x) in the 5-300μg/ml range, with a regression equation of the form y=42.13x-27.79 and a correlation coefficient of 0.9861. Five hybrid rice grain samples were analyzed with results in satisfactory agreement to those obtained by high-performance liquid chromatography. © 2003 Elsevier Inc. All rights reserved.
语种：
英文
展开
Estimating allelic number and identity in state of QTLs in interconnected families
作者：
Jannink, JL* ;Wu, XL
期刊：
GENETICS RESEARCH ,2003年81(2):133-144 ISSN：0016-6723
通讯作者：
Jannink, JL
作者机构：
[Jannink, JL] Iowa State Univ, Dept Agron, Ames, IA 50011 USA.;Hunan Agr Univ, Ctr Life Sci Res, Changsha 410218, Hunan, Peoples R China.
通讯机构：
[Jannink, JL] I;Iowa State Univ, Dept Agron, Ames, IA 50011 USA.
摘要：
When multiple related families derived from inbred lines are jointly analysed to detect quantitative trait loci (QTLs), the analysis should estimate allelic effects as accurately as possible and estimate the probability that different parents carry alleles that are identical in state. Analyses exist that assume that all parents carry unique alleles or that all parents but one carry the same allele. In practice, many configurations are possible that group different parents according to their identity-in-state condition at a putative QTL allele. Here, we propose a variable model Bayesian analysis that selects among possible identity-in-state configurations and jointly estimates the allelic effects of identical-in-state parents. We contrast this analysis with a fixed model analysis that estimates unique allelic effects for all parents. We analyse two simulated mating designs: an experimental design in which three inbred parents were crossed to generate two families of 150 doubled haploid lines; and a breeding design in which 20 inbred parents were crossed to generate 60 families of 20 doubled haploid lines, with each parent contributing to six families. In all cases where some parents were simulated to carry alleles of identical effect (that is, they were identical in state), the variable analysis estimated allelic effects with lower mean-squared error than the fixed analysis. The variable analysis showed that, unless each family contains many individuals (more than 100), there is insufficient information in DNA-marker and phenotypic data to determine with high probability the QTL allelic number.
语种：
英文
展开
Amperometric biosensor with HRP immobilized on a sandwiched nano-Au / polymerized m-phenylenediamine film and ferrocene mediator
作者：
Li, J;Xiao, LT;Liu, XM;Zeng, GM;Huang, GH;...
期刊：
Analytical and Bioanalytical Chemistry ,2003年376(6):902-907 ISSN：1618-2642
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Chem & Chem Engn Coll, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones Hunan Prov, Changsha 410028, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Chem & Chem Engn Coll, Changsha 410082, Peoples R China.
关键词：
Enzyme biosensor;H2O2;m-Phenylenediamine polymer;Nano-Au particle
摘要：
An amperometric biosensor has been developed for the determination of H2O2 in plant samples. Horseradish peroxidase (HRP) is immobilized on a sandwiched nano-Au particle / m-phenylenediamine polymer film by glutaraldehyde cross-linking. The film is formulated on the carbon paste electrode (CPE) blended with ferrocene as an electron transfer mediator. On the low concentration range, the current response is related to the H 2O2 concentration linearly from 0 to 8×10 -6 M with a detection limit of 1.3×10-7 M. On a wider concentration range of 8×10-6 to 1.4×10 -4 M, the reciprocal of current response is linearly related to the reciprocal of H2O2 concentration. The apparent Michaelis-Menten constant (Km app) was calculated to be 0.0334 mM. The sensor has been tested by determining H2O2 concentration in plant leaf samples. © Springer-Verlag 2003.
语种：
英文
展开
A novel piezoelectric biosensor for the detection of phytohormone β-indole acetic acid
作者：
Li, J;Wu, ZY;Xiao, LT;Zeng, GM;Huang, GH;...
期刊：
Analytical Sciences ,2002年18(4):403-407 ISSN：0910-6340
通讯作者：
Shen, GL
作者机构：
[Shen, GL] Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.;Hunan Agr Univ, Key Lab Phytohormones, Changsha 410082, Peoples R China.
通讯机构：
[Shen, GL] H;Hunan Univ, Chem & Chem Engn Coll, Dept Environm Sci & Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China.
摘要：
A novel piezoelectric immunosensor has been developed for the determination of β-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 μg/ml with a regression equation of the form y = - 23x + 151 (r = 0.9937).
语种：
英文
展开
虎纹捕鸟蛛毒素-I单残基突变体R20 A-HWTX-I的化学合成与性质分析
作者：
王贤纯;梁宋平;罗泽民
期刊：
生物工程学报 ,2000年16(4):490-494 ISSN：1000-3061
通讯作者：
Wang, X.C.
作者机构：
[王贤纯; 梁宋平] 湖南师范大学生命科学学院;[罗泽民] 湖南农业大学生物技术系
关键词：
虎纹捕鸟蛛毒素-Ⅰ;突变体;固相多肽合成;蛋白质工程;蜘蛛
摘要：
虎纹捕鸟蛛毒素 Ⅰ （Huwentoxin Ⅰ ,HWTX Ⅰ ）是从虎纹捕鸟蛛 （Selenocosmiahuwena）的粗毒中分离出的一种多肽类神经毒素。为了探明该毒素分子中唯一的Arg残基与其生物学活性的关系 ,运用固相多肽合成技术和Fmoc化学直接构建了Ala取代HWTX Ⅰ第 2 0位Arg（R2 0 ）的突变体R2 0A HWTX Ⅰ ;将合成的突变体置于含谷胱甘肽的缓冲体系中氧化复性后用反相和特殊设计的离子交换HPLC纯化 ,并对之进行氨基酸组成、Edman降解与质谱分析。活性测定结果表明 ,HWTX Ⅰ分子中的R2 0被A取代后 ,活性下降了 92 % ,提示R2 0是与活性密切相关的重要残基。
语种：
中文
展开
