摘要：
Over the past few years, the antitumor activity exhibited by 5-caffeylquinic acid (5-CQA), especially its inhibitory effect on hepatocellular carcinoma (HCC) proliferation and metastasis, has been recognized as a new research hotspot. However, impacted by the weak antitumor toxicity of 5-CQA, its clinical application has been limited. In this study, the antitumor effect arising from 5-CQA on HCC cells was evaluated through cell viability assay. In addition, proteomics, flow cytometry, qRT-PCR and western blotting were adopted to investigate the drug resistance mechanism of HCC cells to 5-CQA. As indicated by the results, 5-CQA significantly inhibited the proliferation of HCC cell lines MHCC97H and HCCLM3 with IC50(48h) of 546.8μM and 452μM, respectively. According to the in-depth studies, the abnormal activation of HIF-1α/glucose transporters/glycolysis pathway of 5-CQA could be a key molecular mechanism leading to drug resistance of HCC cells. Thus, this study found that glucose starvation, glucose analogue 2-DG, hexokinase inhibitor bromopyruvic acid and PKM2 inhibitor compound 3k inhibited HCC cell proliferation in synergy with 5-CQA. Furthermore, though the 5-CQA derivatives methyl chlorogenate (MCGA) and 3,5-dicaffeoylquinic acid (3,5-diCQA) exhibited more potent antiproliferation activity in HCC cells than 5-CQA, they also up-regulated the expression of GLUT1/3, whereas they had no effect on hepatocytes. To be specific, under low-glucose culture conditions, the order of sensitivity of HCC cells to CQAs was 3,5-diCQA>MCGA > 5-CQA. In brief, the above results revealed that intervention in glucose metabolism can facilitate the effects of 5-CQA and its derivatives for treating HCC.
期刊：
Archives of Microbiology,2022年204(6):345 ISSN：0302-8933
通讯作者：
Tian, Y.;Yu, Y.-B.
作者机构：
[Zhou, Jie; Tian, Yun; Liu, Hu-Hu; Pan, Hu; He, Guo-Wei; Wang, Chong; Yang, Hui] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Pan, Hu; Yu, Yao-Bin] Tibet Acad Agr & Anim Husb Sci, Inst Agr Prod Qual Stand & Testing Res, Lhasa 850032, Peoples R China.;[Zhou, Zi-Qiong] Tibet Inst Agr & Anim Husb, Sch Food Sci, Nyingchi 860000, Peoples R China.;[Jin, Kai] Agr Technol Extens Serv Ctr Tibet, Lhasa 850032, Peoples R China.
通讯机构：
[Yao-Bin Yu] I;[Yun Tian] C;Institute of Agricultural Product Quality Standard and Testing Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
作者：
Du, Yuanchun;Ke, Zhenyi;Zhang, Jiaheng;Feng, Guangfu
期刊：
Biosensors and Bioelectronics,2022年216:114656 ISSN：0956-5663
通讯作者：
Jiaheng Zhang<&wdkj&>Guangfu Feng
作者机构：
[Zhang, Jiaheng; Du, Yuanchun] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.;[Feng, Guangfu; Ke, Zhenyi] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Zhang, Jiaheng] Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China.
通讯机构：
[Jiaheng Zhang; Guangfu Feng] S;State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China<&wdkj&>College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China<&wdkj&>School of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China
关键词：
CPNs;Cholesterol;Colorimetric-temperature dual-signal;Point-of-care;Test paper
摘要：
Paper-based point-of-care (POC) devices exhibit the advantages of simplicity, rapidity, trim sizes, and low cost, which are of particular importance for food safety, biological analysis, and medical diagnosis. However, the materials utilized to make paper-based POC rarely produce multiple signals, hampering further applications in diverse situations. Herein, we present an appealing approach, namely Colorimetric-Temperature Dual-Signal Output Sensor (CTDSS), and construct a CTDSS based on coordinative self-assembly biomimetic nanozymes Fe-GMP-L-His CPNs as a proof of concept. These CPNs mimic the structure of horseradish peroxidase (HRP), in which Fe (II) is the center, nucleotide GMP and histidine are chosen as ligands to simulate metal coordination of the pyrrole ring and protein function in HRP, respectively. This strategy allows CPNs to show an excellent peroxidase-like activity, efficiently converting H(2)O(2) into •OH and oxidizing TMB to generate colorimetric-temperature dual-signal. As a proof-of-concept application, we exploited cholesterol as the target and successfully applied this CTDSS to detect cholesterol, displaying extraordinary features of rapidity, dramatic specificity, and high sensitivity. By utilizing the colorimetric test strip and temperature discoloration sticker, the paper-based POC tools were constructed to visualize the target. Meanwhile, two proposed test strip POC devices generating different signal outputs exhibited remarkable feasibility and were further employed to detect cholesterol in human serum. We anticipate that this CTDSS platform will inspire innovative concepts for future portable detection tools.
通讯机构：
[Suhong Yu; Zai-Sheng Wu] C;Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350002, China
通讯机构：
[Jia-Shi Peng; Shuan Meng] A;Authors to whom correspondence should be addressed.<&wdkj&>School of Life and Health Sciences, Hunan University of Science and Technology, Xiangtan 411201, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Rice Stress Biology, Changsha 410128, China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha 410128, China
关键词：
Cd accumulation;Cd tolerance;S. plumbizincicola;ZIP2
摘要：
Zinc (Zn)-regulated and iron (Fe)-regulated transporter-like proteins (ZIP) are key players involved in the accumulation of cadmium (Cd) and Zn in plants. Sedum plumbizincicola X.H. Guo et S.B. Zhou ex L.H. Wu (S. plumbizincicola) is a Crassulaceae Cd/Zn hyperaccumulator found in China, but the role of ZIPs in S. plumbizincicola remains largely unexplored. Here, we identified 12 members of ZIP family genes by transcriptome analysis in S. plumbizincicola and cloned the SpZIP2 gene with functional analysis. The expression of SpZIP2 in roots was higher than that in the shoots, and Cd stress significantly decreased its expression in the roots but increased its expression in leaves. Protein sequence characteristics and structural analysis showed that the content of alanine and leucine residues in the SpZIP2 sequence was higher than other residues, and several serine, threonine and tyrosine sites can be phosphorylated. Transmembrane domain analysis showed that SpZIP2 has the classic eight transmembrane regions. The evolutionary analysis found that SpZIP2 is closely related to OsZIP2, followed by AtZIP11, OsZIP1 and AtZIP2. Sequence alignment showed that most of the conserved sequences among these members were located in the transmembrane regions. A further metal sensitivity assay using yeast mutant Δyap1 showed that the expression of SpZIP2 increased the sensitivity of the transformants to Cd but failed to change the resistance to Zn. The subsequent ion content determination showed that the expression of SpZIP2 increased the accumulation of Cd in yeast. Subcellular localization showed that SpZIP2 was localized to membrane systems, including the plasma membrane and endoplasmic reticulum. The above results indicate that ZIP member SpZIP2 participates in the uptake and accumulation of Cd into cells and might contribute to Cd hyperaccumulation in S. plumbizincicola.
摘要：
Iron-oxidizing strain (FeOB) and iron modified biochars have been shown arsenic (As) remediation ability in the environment. However, due to the complicated soil environment, few field experiment has been conducted. The study was conducted to investigate the potential of iron modified biochar (BC-FeOS) and biomineralization by a new found FeOB to remediate As-contaminated paddy field. Compared with the control, the As contents of G(B)(BC-FeOS), G(F) (FeOB), G FN (FeOB and nitrogen fertilizer), G(BF) (BC-FeOS and FeOB) and G(BFN) (BC-FeOS, FeOB and nitrogen fertilizer) treatments in pore water decreased by 36.53%-80.03% and the microbial richness of iron-oxidizing bacteria in these treatments increased in soils at the rice maturation stage. The concentrations of available As of G(B), G(F), G(FN), G(BF) and G(BFN) at the tillering stage were significantly decreased by 10.78%-55.48%. The concentrations of nonspecifically absorbed and specifically absorbed As fractions of G(B), G(F), G(FN), G(BF) and G(BFN) in soils were decreased and the amorphous and poorly crystalline hydrated Fe and Al oxidebound fraction was increased. Moreover, the As contents of G(B), G(F), G(FN), G(BF) and G(BFN) in rice grains were significantly decreased (*P < 0.05) and the total As contents of G(FN), G(BF) and G(BFN) were lower than the standard limit of the National Standard for Food Safety (GB 2762-2017). Compared with the other treatments, G(BFN) showed the greatest potential for the effective remediation of As-contaminated paddy fields. (C) 2021 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
通讯机构：
[Wenbang Tang; Xuedan Lu] C;College of Agronomy, Hunan Agricultural University, Changsha 410128, PR China<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha 410128, PR China<&wdkj&>State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, 410125, PR China
关键词：
Energy saving;Factory nursery;Pc-LEDs;Tobacco
摘要：
Industrial Seedling Raising (ISR) is increasingly becoming an important part of Modern Agriculture because of its efficient utilization of land, water, and fertilizer as well as its advantages of being not easily affected by the weather. However, the high cost and high energy consumption of light sources for plant growth is limiting the popularization of ISR technology. Phosphor-converted light-emitting diodes (pc-LEDs) make use of relatively affordable red phosphor and blue light chips, providing an adjustable spectrum to optimize plant growth. To identify the energy-saving light quality of pc-LEDs, we investigated the effects of a variety of light qualities on the growth of tobacco seedlings. Y3Al5O12:Ce3+, CaAlSiN3:Eu2+, KAl11O17:Eu2+ phosphors were combined with the blue light chip according to different proportions to produce the following light sources: CK (white light), T1 (blue light), T2 (red light), T3 (red: blue light ratio = 1:4), T4 (red: blue light ratio = 4:1). The tobacco variety Xiangyan7 grown continuously under T1, T2, T3, and T4 significantly increased the leaf area, stem length, biomass, root area and main root length compared with those grown under white light. Among the five kinds of light qualities tested, T4 treatment exerted the best effect on leaf development and biomass increase, while T2 exerted the best effect on stem elongation. The cytological analysis demonstrated that the promotion of the cell size and cell number of leaf epidermal cells by T1-T4 might contribute to the leaf expansion. Further analysis at the molecular level suggested that the light quality affected the RNA levels of the genes involved in cell division and expansion. When tobacco seedlings reached the same biomass, T1-T4 light sources saved 71%, 86%, 80% and 89% of electric energy respectively compared with white light. Therefore, the application of specific pc-LEDs not only reduces the cost of light source production, but also saves energy consumption, offering great potential for ISR technology to cut costs and increase efficiency.
作者机构：
[Liu, Yannan; Deng, Xing Wang; Yin, Xiaochang; Shu, Jia; Zhu, Danmeng; Sun, Linhua; Qian, Weiqiang; Cao, Yuxin] Peking Univ, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China.;[Wu, Songlin; Wang, Jiayin; Ye, Keqiong] Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China.;[Wu, Songlin; Wang, Jiayin; Ye, Keqiong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Dai, Xing] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.;[Deng, Xing Wang; Sun, Linhua; Qian, Weiqiang] Peking Univ, Inst Adv Agr Sci, Weifang 261325, Shandong, Peoples R China.
通讯机构：
[Weiqiang Qian] S;State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences, Peking University , Beijing 100871, China<&wdkj&>Peking University Institute of Advanced Agricultural Sciences , Weifang, Shandong 261325, China
摘要：
Small nucleolar RNAs (snoRNAs) are noncoding RNAs (ncRNAs) that guide chemical modifications of structural RNAs, which are essential for ribosome assembly and function in eukaryotes. Although numerous snoRNAs have been identified in plants by high-throughput sequencing, the biological functions of most of these snoRNAs remain unclear. Here, we identified box C/D SnoR28.1s as important regulators of plant growth and development by screening a CRISPR/Cas9-generated ncRNA deletion mutant library in Arabidopsis thaliana. Deletion of the SnoR28.1 locus, which contains a cluster of three genes producing SnoR28.1s, resulted in defects in root and shoot growth. SnoR28.1s guide 2′-O-ribose methylation of 25S rRNA at G2396. SnoR28.1s facilitate proper and efficient pre-rRNA processing, as the SnoR28.1 deletion mutants also showed impaired ribosome assembly and function, which may account for the growth defects. SnoR28 contains a 7-bp antisense box, which is required for 2′-O-ribose methylation of 25S rRNA at G2396, and an 8-bp extra box that is complementary to a nearby rRNA methylation site and is partially responsible for methylation of G2396. Both of these motifs are required for proper and efficient pre-rRNA processing. Finally, we show that SnoR28.1s genetically interact with HIDDEN TREASURE2 and NUCLEOLIN1. Our results advance our understanding of the roles of snoRNAs in Arabidopsis.
摘要：
Watermelon (Citrullus lanatus) is one of the most popular fruit crops. However, Fusarium wilt (FW) is a serious soil-borne disease caused by Fusarium oxysporum f. sp. niveum (FON) that severely limits the development of the watermelon industry. Trichoderma spp. is an important plant anti-pathogen biocontrol agent. The results of our previous study indicated that Trichoderma asperellum M45a (T. asperellum M45a) could control FW by enhancing the relative abundance of plant growth-promoting rhizobacteria (PGPR) in the rhizosphere of watermelon. However, there are few studies on its mechanism in the pathogen resistance of watermelon. Therefore, transcriptome sequencing of T. asperellum M45a-treated watermelon roots combined with metabolome sequencing of the rhizosphere soil was performed with greenhouse pot experiments. The results demonstrated that T. asperellum M45a could stably colonize roots and significantly increase the resistance-related enzymatic activities (e.g., lignin, cinnamic acid, peroxidase and peroxidase) of watermelon. Moreover, the expression of defense-related genes such as MYB and PAL in watermelon roots significantly improved with the inoculation of T. asperellum M45a. In addition, KEGG pathway analysis showed that a large number of differentially expressed genes were significantly enriched in phenylpropane metabolic pathways, which may be related to lignin and cinnamic acid synthesis, thus further inducing the immune response to resist FON. Furthermore, metabolic analysis indicated that four differential metabolic pathways were enriched in M45a-treated soil, including six upregulated compounds and one down-regulated compound. Among them, galactinol and urea were significantly positively correlated with Trichoderma. Hence, this study provides insight into the biocontrol mechanism of T. asperellum M45a to resist soil-borne diseases, which can guide its industrial application.
通讯机构：
[Zhi Liu] C;[Junhua Chen] N;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China<&wdkj&>National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management, Institute of Eco-Environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, China
作者机构：
[Tang, Shengsong; Tian, Xiaoyan; Yu, Jianqiang] Ningxia Med Univ, Dept Pharmacol, Yinchuan 750004, Peoples R China.;[Tang, Shengsong; Tian, Xiaoyan; Ning, Qian] Hunan Univ Med, Sch Pharmaceut Sci, Hunan Prov Key Lab Antibody Based Drug & Intellige, Huaihua 418000, Peoples R China.;[Tang, Shengsong; Ning, Qian] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Tang, Shengsong] Ningxia Med Univ, Sch Pharmacol, Yinchuan 750004, Peoples R China.
通讯机构：
[Jianqiang Yu] D;Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China
关键词：
Cancer immunotherapy;Immune checkpoint;Regulatory T cells;TIGIT
摘要：
T-cell immunoglobulin and ITIM domain (TIGIT) is a novel type of immune checkpoint. Importantly, immune checkpoint molecules promote cancer progression by various antitumor suppressive mechanisms. TIGIT is an inhibitory receptor expressed on T cells, natural killer cells, and regulatory T cells that was recently attracted attention as a major emerging target for cancer immunotherapy. Regulatory T cells (Tregs) play crucial roles in immune homeostasis. Specifically, tumor-infiltrating Tregs promote cancer progression by restricting antitumor immunity and supporting tumor immune escape. In this review, we summarized the current understanding on TIGIT and tumor-infiltrating Tregs. Here, we reviewed the latest advances in the understanding of mechanisms causing tumor-infiltrating Tregs abundance to optimize Tregs targeted therapy. Collectively, anti-TIGIT targeting Tregs hold great promise for potent cancer target therapy.
通讯机构：
[Weiping Wang; Ruozhong Wang] A;Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
关键词：
leaf senescence;ROS;cell death;LS1;rice
摘要：
Premature senescence of leaves causes a reduced yield and quality of rice by affecting plant growth and development. The regulatory mechanisms underlying early leaf senescence are still unclear. The Leaf senescence 1 (LS1) gene encodes a C2H2-type zinc finger protein that is localized to both the nucleus and cytoplasm. In this study, we constructed a rice mutant named leaf senescence 1 (ls1) with a premature leaf senescence phenotype using CRISPR/Cas9-mediated editing of the LS1 gene. The ls1 mutants exhibited premature leaf senescence and reduced chlorophyll content. The expression levels of LS1 were higher in mature or senescent leaves than that in young leaves. The contents of reactive oxygen species (ROS), malondialdehyde (MDA), and superoxide dismutase (SOD) were significantly increased and catalase (CAT) activity was remarkably reduced in the ls1 plants. Furthermore, a faster decrease in pigment content was detected in mutants than that in WT upon induction of complete darkness. TUNEL and staining experiments indicated severe DNA degradation and programmed cell death in the ls1 mutants, which suggested that excessive ROS may lead to leaf senescence and cell death in ls1 plants. Additionally, an RT-qPCR analysis revealed that most senescence-associated and ROS-scavenging genes were upregulated in the ls1 mutants compared with the WT. Collectively, our findings revealed that LS1 might regulate leaf development and function, and that disruption of LS1 function promotes ROS accumulation and accelerates leaf senescence and cell death in rice.
通讯机构：
[Sheng Zhang; Jianan Huang; Zhonghua Liu] K;Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China<&wdkj&>National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals and Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, Hunan, China<&wdkj&>Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Hunan Agricultrual University, Changsha 410128, China<&wdkj&>Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, Hunan, China<&wdkj&>National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals and Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, Hunan, China
摘要：
Persistent inflammatory infiltration of skeletal muscle is a principal trigger for the loss of muscle mass and strength. Theaflavins, the main functional components of black tea, have effects on muscle health, but their biological effects on skeletal muscle inflammation are unclear. We constructed in vitro and in vivo models of muscle inflammation and found that theaflavins reduced the expression of inflammatory factors (IL-1β, IL-6, and TNF-α) by regulating the TLR4/MyD88/NF-κB signaling pathway to alleviate muscle inflammation. In addition, TF1 can regulate the metabolic function of skeletal muscle under inflammatory conditions, reduce the content of proinflammatory substances, improve the mechanical properties (stiffness and roughness) of the surface of inflammatory myotubes, and promote the recovery of muscle after an inflammatory injury. In conclusion, theaflavins may serve as a diet-derived anti-inflammatory factor with potential modulatory effects on skeletal muscle metabolism and mechanical properties in an inflammatory environment.
