摘要：
The ADP-ribosylation factor 6 (Arf6), as the only member of the Arf family III protein, has been extensively studied for its diverse biological functions in animals. Previously, the Arf6 protein in Magnaporthe oryzae was found to be crucial for endocytosis and polarity establishment during asexual development. However, its role remains unclear in S. sclerotiorum. Here, we identified and characterized SsArf6 in S. sclerotiorum using a reverse genetic approach. Deletion of SsArf6 impaired hyphal growth and development and produced more branches. Interestingly, knockout of SsArf6 resulted in an augmented tolerance of S. sclerotiorum towards oxidative stress, and increased its sensitivity towards osmotic stress, indicative of the different roles of SsArf6 in various stress responses. Simultaneously, SsArf6 deletion led to an elevation in melanin accumulation. Moreover, the appressorium formation was severely impaired, and fungal virulence to host plants was significantly reduced. Overall, our findings demonstrate the essential role of SsArf6 in hyphal development, stress responses, appressorium formation, and fungal virulence to host plants.
期刊：
Drug Design, Development and Therapy,2024年18:699-717 ISSN：1177-8881
通讯作者：
Guo, C
作者机构：
[Zhou, Xuqing; Guo, C; Wang, Xu; Guo, Chun; Lei, Shihui; Yang, Yi] Hunan Univ Chinese Med, Hosp 1, Expt Ctr Med Innovat, Changsha 410007, Hunan, Peoples R China.;[Zhou, Xuqing; Guo, C; Wang, Xu; Guo, Chun] Hunan Univ Chinese Med, Clin Coll Tradit Chinese Med 1, Changsha 410007, Hunan, Peoples R China.;[Li, Jiaqi; He, Ying; Yang, Hua] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Zhang, Mengxue; Zhou, Desheng] Hunan Univ Chinese Med, Hosp 1, Dept Neurol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Guo, C ] H;Hunan Univ Chinese Med, Hosp 1, Expt Ctr Med Innovat, Changsha 410007, Hunan, Peoples R China.;Hunan Univ Chinese Med, Clin Coll Tradit Chinese Med 1, Changsha 410007, Hunan, Peoples R China.
摘要：
BACKGROUND: Annao Pingchong decoction (ANPCD) is a traditional Chinese decoction which has definite effects on treating intracerebral hemorrhage (ICH) validated through clinical and experimental studies. However, the impact of ANPCD on oxidative stress (OS) after ICH remains unclear and is worth further investigating. AIM: To investigate whether the therapeutic effects of ANPCD on ICH are related to alleviating OS damage and seek potential targets for its antioxidant effects. MATERIALS AND METHODS: The therapeutic candidate genes of ANPCD on ICH were identified through a comparison of the target genes of ANPCD, target genes of ICH and differentially expressed genes (DEGs). Protein-protein interaction (PPI) network analysis and functional enrichment analysis were combined with targets-related literature to select suitable antioxidant targets. The affinity between ANPCD and the selected target was verified using macromolecular docking. Subsequently, the effects of ANPCD on OS and the selected target were further investigated through in vivo experiments. RESULTS: Forty-eight candidate genes were screened, in which silent information regulator sirtuin 1 (SIRT1) is one of the core genes that has antioxidant effects and ICH significantly affected its expression. The good affinity between 6 compounds of ANPCD and SIRT1 was also demonstrated by macromolecular docking. The results of in vivo experiments demonstrated that ANPCD significantly decreased modified neurological severity scoring (mNSS) scores and serum MDA and 8-OHdG content in ICH rats, while significantly increasing serum SOD and CAT activity, complicated with the up-regulation of ANPCD on SIRT1, FOXO1, PGC-1α and Nrf2. Furthermore, ANPCD significantly decreased the apoptosis rate and the expression of apoptosis-related proteins (P53, cytochrome c and caspase-3). CONCLUSION: ANPCD alleviates OS damage and apoptosis after ICH in rats. As a potential therapeutic target, SIRT1 can be effectively regulated by ANPCD, as are its downstream proteins.
摘要：
Squalene is an important triterpene with a wide range of applications. Given the growing market demand for squalene, the development of microbial cell factories capable of squalene production is considered a sustainable method. This study aimed to investigate the squalene production potential of Yarrowia lipolytica. First, HMG-CoA reductase from Saccharomyces cerevisiae and squalene synthase from Y. lipolytica was co-overexpressed in Y. lipolytica. Second, by enhancing the supply of NADPH in the squalene synthesis pathway, the production of squalene in Y. lipolytica was effectively increased. Furthermore, by constructing an isoprenol utilization pathway and overexpressing YlDGA1, the strain YLSQ9, capable of producing 868.1 mg/L squalene, was obtained. Finally, by optimizing the fermentation conditions, the highest squalene concentration of 1628.2 mg/L (81.0 mg/g DCW) in Y. lipolytica to date was achieved. This study demonstrated the potential for achieving high squalene production using Y. lipolytica.
摘要：
Honey is well-known as a food product that is rich in active ingredients and is very popular among consumers. Free amino acids (FAAs) are one of the important nutritional components of honey, which can be used not only as a nutritional indicator of honey but also as an indicator of plant source identification. In this study, the contents of 20 FAAs in seven types of honey from 11 provinces in China were examined for the first time. The 20 FAAs were analyzed by ultra-performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS). By analyzing 93 honey samples from seven types of honey, the FAAs were found to range from 394.4 mg/kg (linden honey) to 1771.7 mg/kg (chaste honey). Proline ranged from 274.55 to 572.48 mg/kg, and methionine was only present in some of the linden honey, chaste honey, acacia honey, and rape honey. Evaluated by amino acid principal component analysis, multifloral grassland honey had the highest overall evaluation score, acacia and jujube honey were the most similar, while chaste honey was the least similar to the other types of honey. In addition, DNA was extracted from 174 Xinjiang grassland honey samples and different plant leaves for PCR and sequencing to identify the species of nectar plants. As a result, 12 families and 25 species of honey plants were identified. The results confirmed the diversity of FAAs in dissimilar types and sources of honey. This study provides a reference for expanding honey quality standards and verifying the authenticity of honey.
摘要：
Ulcerative colitis (UC) is a chronic, non-specific inflammatory sickness of the intestinal tract, chiefly implicating the rectum and colon, which is characterized by chronic or subacute diarrhea, mucopurulent stools, and abdominal pain. The pathogeny of UC is still uncertain, and it is thought that multiple factors interact to cause the disease, such as environment, genetics, gut microbes, and immunity. Injuring the intestinal barrier is one of the most significant features of UC and includes mechanical, chemical, immune, and biological barriers. Plenty of research has shown that probiotics, as profitable bacteria in the gut, can play a prominent role in the treatment of UC by improving gut barrier function and modulating gut immunity. Lactobacillus plantarum (L. plantarum), a common probiotic, has made outstanding contributions to food and medicine, and many studies in recent years have shown that L. plantarum has great preventive and therapeutic effects on ulcerative colitis and restores the intestinal barrier. This paper reviews the mechanisms of L. plantarum for improving the intestinal barrier function of UC organisms, mainly including regulating the immune response, inhibiting oxidative stress, raising the expression of tight junction (TJ) proteins, promoting the formation of mucin, improving the composition of gut flora, and raising the levels of short-chain fatty acids (SCFAs), which offers some help for the clinical therapy of UC.
期刊：
PLANT CELL AND ENVIRONMENT,2024年 ISSN：0140-7791
通讯作者：
Zhang, HW;Xiao, GQ
作者机构：
[Zhang, Zhijin; Chen, Yating; Chen, Ying; Zhang, Haiwen; Jiang, Xiaodan; Zhai, Mingjuan; Zhou, Jiahao] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China.;[Xiao, Guiqing; Chen, Yating] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
通讯机构：
[Xiao, GQ ] H;[Zhang, HW ] C;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
关键词：
Oryza sativa L;ethylene signal;low temperature response;transcriptional regulation
摘要：
Low temperature severely affects rice development and yield. Ethylene signal is essential for plant development and stress response. Here, we reported that the OsEIN2-OsEIL1/2 pathway reduced OsICE1-dependent chilling tolerance in rice. The overexpressing plants of OsEIN2, OsEIL1 and OsEIL2 exhibited severe stress symptoms with excessive reactive oxygen species (ROS) accumulation under chilling, while the mutants (osein2 and oseil1) and OsEIL2-RNA interference plants (OsEIL2-Ri) showed the enhanced chilling tolerance. We validated that OsEIL1 and OsEIL2 could form a heterxodimer and synergistically repressed OsICE1 expression by binding to its promoter. The expression of OsICE1 target genes, ROS scavenging- and photosynthesis-related genes were downregulated by OsEIN2 and OsEIL1/2, which were activated by OsICE1, suggesting that OsEIN2-OsEIL1/2 pathway might mediate ROS accumulation and photosynthetic capacity under chilling by attenuating OsICE1 function. Moreover, the association analysis of the seedling chilling tolerance with the haplotype showed that the lower expression of OsEIL1 and OsEIL2 caused by natural variation might confer chilling tolerance on rice seedlings. Finally, we generated OsEIL2-edited rice with an enhanced chilling tolerance. Taken together, our findings reveal a possible mechanism integrating OsEIN2-OsEIL1/2 pathway with OsICE1-dependent cascade in regulating chilling tolerance, providing a practical strategy for breeding chilling-tolerant rice.
关键词：
Cd uptake and efflux;Gene prediction;Microbial community;Migration and transformation
摘要：
Cadmium (Cd)-contaminated soil poses a severe threat to crop production and human health, while also resulting in a waste of land resources. In this study, two types of organic fertilizer (ZCK: Low-content available iron; Z2: High-content available iron) were applied to Cd-contaminated soil for rice cultivation, and the effects of the fertilizer on rice growth and Cd passivation were investigated in conjunction with soil microbial analysis. Results showed that Z2 could alter the composition, structure, and diversity of microbial communities, as well as enhance the complexity and stability of the microbial network. Both 2% and 5% Z2 significantly increased the fresh weight and dry weight of rice plants while suppressing Cd absorption. The 2% Z2 exhibited the best Cd passivation effect. Gene predictions suggested that Z2 may promote plant growth by regulating microbial production of organic acids that dissolve phosphorus and potassium. Furthermore, it is suggested that Z2 may facilitate the absorption and immobilization of soil cadmium through the regulation of microbial cadmium efflux and uptake systems, as well as via the secretion of extracellular polysaccharides. In summary, Z2 can promote rice growth, suppress Cd absorption by rice, and passivate soil Cd by regulating soil microbial communities.
关键词：
Spent mushroom compost;Nitrogen loss;Microbial community structure;Core microorganisms;Nitrogen metabolism;Chicken manure
摘要：
Due to the rapid development of animal husbandry, the associated environmental problems cannot be ignored, with the management of livestock and poultry manure emerging as the most prominent issue. Composting technology has been widely used in livestock and poultry manure management. A deeper understanding of the nitrogen conversion process during composting offers a theoretical foundation for selecting compost substrates. In this study, the effects of sawdust (CK) and spent mushroom compost (T1) as auxiliary materials on nitrogen as well as microbial structure in the composting process when composted with chicken manure were investigated. At the end of composting, the nitrogen loss of T1 was reduced by 17.18% relative to CK. When used as a compost substrate, spent mushroom compost accelerates the succession of microbial communities within the compost pile and alters the core microbial communities within the microbial community. Bacterial genera capable of cellulose degradation (Fibrobacter, Herbinix) are new core microorganisms that influence the assimilation of nitrate reduction during compost maturation. Using spent mushroom compost as a composting substrate increased the enzyme activity of nitrogen assimilation while decreasing the enzyme activity of the denitrification pathway.
摘要：
The components of a protein complex involved in recognizing the histone modification H3K27me3 and transcriptional repression function in an epigenetic module that negatively regulates the N‐hydroxypipecolic acid biosynthesis gene FLAVIN‐DEPENDENT MONOOXYGENASE 1. ABSTRACT N‐Hydroxypipecolic acid (NHP) is a signaling molecule crucial for systemic acquired resistance (SAR), a systemic immune response in plants that provides long‐lasting and broad‐spectrum protection against secondary pathogen infections. To identify negative regulators of NHP biosynthesis, we performed a forward genetic screen to search for mutants with elevated expression of the NHP biosynthesis gene FLAVIN‐DEPENDENT MONOOXYGENASE 1 (FMO1). Analysis of two constitutive expression of FMO1 (cef) and one induced expression of FMO1 (ief) mutants revealed that the AIPP3–PHD2–CPL2 protein complex, which is involved in the recognition of the histone modification H3K27me3 and transcriptional repression, contributes to the negative regulation of FMO1 expression and NHP biosynthesis. Our study suggests that epigenetic regulation plays a crucial role in controlling FMO1 expression and NHP levels in plants.
摘要：
HYPOTHESIS: Construction of dual gatekeepers-functionalized mesoporous organic silica nanoparticles (MONs) with both physical and chemical mechanisms for modulated drug delivery properties provides one solution to the extracellular stability vs. intracellular high therapeutic efficiency of MONs that hold great potential for clinical translations. EXPERIMENTS: We reported herein facile construction of diselenium-bridged MONs decorated with dual gatekeepers, i.e., azobenzene (Azo)/polydopamine (PDA) for both physical and chemical modulated drug delivery properties. Specifically, Azo can act as a physical barrier to block DOX in the mesoporous structure of MONs for extracellular safe encapsulation. The PDA outer corona serves not only as a chemical barrier with acidic pH-modulated permeability for double insurance of minimized DOX leakage in the extracellular blood circulation but also for inducing a PTT effect for synergistic PTT and chemotherapy of breast cancer. FINDINGS: An optimized formulation, DOX@(MONs-Azo3)@PDA resulted in approximately 1.5 and 2.4 fold lower IC50 values than DOX@(MONs-Azo3) and (MONs-Azo3)@PDA controls in MCF-7 cells, respectively, and further mediated complete tumor eradication in 4T1 tumor-bearing BALB/c mice with insignificant systematic toxicity due to the synergistic PTT and chemotherapy with enhanced therapeutic efficiency.
摘要：
Cordycepin, a nucleoside compound with a variety of biological activities, has been extensively applied in the nutraceutical and pharmaceutical industries. The advancement of microbial cell factories using agro-industrial residues provides a sustainable pathway for cordycepin biosynthesis. Herein, the cordycepin production was enhanced by the modification of glycolysis and pentose phosphate pathway in engineered Yarrowia lipolytica. Then, cordycepin production based on economical and renewable substrates (sugarcane molasses, waste spent yeast, and diammonium hydrogen phosphate) was analyzed. Furthermore, the effects of C/N molar ratio and initial pH on cordycepin production were evaluated. Results indicated that the maximum cordycepin productivity of 656.27mg/L/d (72h) and cordycepin titer was 2286.04mg/L (120h) by engineered Y. lipolytica in the optimized medium, respectively. The cordycepin productivity in the optimized medium was increased by 28.81% compared with the original medium. This research establishes a promising way for efficient cordycepin production from agro-industrial residues.
通讯机构：
[Tongcheng Fu] C;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China<&wdkj&>Hunan Engineering Laboratory of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, China<&wdkj&>National Energy R&D Center for Non-Food Biomass, Hunan Agricultural University, Changsha, China
关键词：
marginal land;soil aggregate;soil carbon sequestration;soil ecology;soil iron and aluminum oxide;soil microbe
摘要：
Long‐term cultivation of Miscanthus and Saccharum arundinaceum increased soil organic carbon stock in infertile red soils. Soil pH is the main factor affecting soil carbon stock in red soils. Soil copiotrophic and oligotrophic microbes significantly shaped by the cultivation of perennial grasses. Abstract Determining the effect of perennial energy crop (PEC) cultivation on soil organic carbon (SOC) in marginal land soil is vital for carbon neutrality and bioeconomy development. However, a comprehensive and systematic evaluation of the response of SOC content to different PECs and its underlying drivers is still lacking. We used soil data collected from infertile red topsoil (0–20 cm) after 10 years of cultivation with Miscanthus (MS), Panicum virgatum (SG), and Saccharum arundinaceum (SA) to explore the changes in SOC stock induced by PEC. The roles of physical, chemical, and microbiological factors driving the increase in the SOC stock were investigated. Results revealed that SA and MS enhanced SOC stock by 87.97% and 27.52% relative to the uncultivated control. Conversely, PEC increased the percentage of soil mega‐aggregates, geometric mean diameters, soil chelate iron (Fe), and aluminum (Al) oxides, and reduced soil acidity for the infertile red soils. In addition, fungal richness and diversity for PEC soils were enhanced compared to the unplanted soil. It is possible that PEC cultivation reduced the relative abundance of copiotrophic fungi but increased the relative abundance of oligotrophic fungi. Furthermore, variance partitioning analysis revealed that chemical and microbiological factors accounted for 80.54% of the total variation for the SOC stock. The partial least squares path model showed that PEC cultivation enhanced soil carbon (C) stock via soil deacidification and increased soil bacterial function. In conclusion, this study confirms the SOC sequestration potential of PEC cultivation in marginal land and the underlying mechanism driving SOC stock. The main positive factors controlling soil C sequestration included “pH,” while the negative factors were “bacterial community,” “fungal community,” and “bacterial function.” Our research may help encourage and support decision‐makers of wasted marginal land conversion to PEC cultivation.
摘要：
From the aerobic pond of the farm, the Pseudomonas sp. G16 was screened and isolated, which was confirmed to exhibit heterotrophic nitrification and aerobic denitrification. The removal rates of Ammonia (100mg/L), nitrate (120mg/L), and nitrite (100mg/L) by the strain were 94.13%, 92.62%, and 85.67%, and the nitrogen metabolism pathway of strain G16 was analyzed by whole genome sequencing combined with its nitrification-denitrification intermediate products, it was found that the strain had independent nitrification-denitrification ability and no nitrite accumulation. Under the conditions of carbon source of sodium succinate hexahydrate, C/N ratio of 15, pH of 7.5, temperature of 15°C, and DO of 210rpm, strain G16 showed excellent denitrification performance. Strain G16 was prepared into biochar-based immobilized bacterial particles, which successfully improved its nitrogen removal efficiency and stability. Therefore, the application of strain G16 in the field of real wastewater treatment has very necessary research value.
摘要：
Chalkiness is a key determinant that directly affects the appearance and cooking quality of rice grains. Previously, Floury endosperm 2 (FLO2) was reported to be involved in the formation of rice chalkiness; however, its regulation mechanism is still unclear. Here, FLO2 interaction factor 3 (OsFIF3), a bHLH transcription factor, was identified and analyzed in Oryza sativa. A significant increase in chalkiness was observed in OsFIF3-overexpressed grains, coupled with a round, hollow filling of starch granules and reduced grain weight. OsFIF3 is evolutionarily conserved in monocotyledons, but variable in dicotyledons. Subcellular localization revealed the predominant localization of OsFIF3 in the nucleus. The DAP-seq (DNA affinity purification sequencing) results showed that OsFIF3 could affect the transcriptional accumulation of β-amylase 1, α-amylase isozyme 2A-like, pectinesterase 11, β-glucosidase 28 like, pectinesterase, sucrose transport protein 1 (SUT1), and FLO2 through the binding of the CACGTG motif on their promoters. Moreover, FLO2 and SUT1 with abundant OsFIF3 binding signals showed significant expression reduction in OsFIF3 overexpression lines, further confirming OsFIF3's role in starch metabolism regulation and energy material allocation. Taken together, these findings show that the overexpression of OsFIF3 inhibits the expression of FLO2 and SUT1, thereby increasing grain chalkiness and affecting grain weight.
作者机构：
[Chen, Zhiqun; Yan, Li; Feng, Guifang] Linyi Univ, Coll Life Sci, Linyi 276000, Peoples R China.;[Feng, Guifang] Philippine Christian Univ, Ctr Int Educ, Manila 1004, Philippines.;[Hao, Xiaodong; Zhu, Ping] Linyi Univ, Coll Resources & Environm, Linyi 276000, Peoples R China.;[Xiao, Yunhua] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Yunhua Xiao; Xiaodong Hao] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Resources and Environment, Linyi University, Linyi 276000, China
关键词：
Cd-contaminated soils;mixotrophic acidophiles;free and attached communities;microbial diversity;Cd removal
摘要：
In this study, cadmium (Cd)-contaminated soils were bioremediated using the mixotrophic acidophiles in different scale-up cultivation stages. The free and attached bacterial and fungal diversities and community structures, and their effects on soil Cd removal were investigated. Percentages of dominant bacteria (Acidithiobacillus, Sulfobacillus, and Pseudomonas) and fungi (Acidomyces and Coniochaeta) between the free and attached communities exhibited the pronounced differences. The alpha-diversity of attached bacteria was higher than the free bacteria, while the distinct variations in attached and free fungi were not observed. However, the beta-diversity between the free and attached bacteria and fungi was significantly changed. Solution pH and reduced glucose level affected the abundance patterns of Acidithiobacillus in free bacteria and Acidomyces in attached fungi, which had great contributions to the soil Cd removal efficiency.
作者机构：
[Sun, Wei; Gao, Shuai; Li, Shuzhen; Su, Guirong; He, Zhiguo] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Su, Guirong] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[He, ZG; He, Zhiguo] Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;[Zhong, Hui; Zhong, H] Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
通讯机构：
[He, ZG ] A;[Zhong, H ] C;Aerosp Kaitian Environm Technol Co Ltd, Changsha 410100, Peoples R China.;Cent South Univ, Sch Life Sci, Changsha 410083, Peoples R China.
摘要：
Pine oil, commonly used as a flotation frother, could affect the bioleaching process. The attachment and biofilm development of acidophiles on metal sulfides were essential for bioleaching. This study focused on the role of pine oil in the early adhesion and biofilm development by Acidianus manzaensis YN-25 on the chalcopyrite surface. It was revealed that pine oil had a negative effect on the adhesion amount of A. manzaensis on the chalcopyrite surface. After having reached adhesion equilibrium, the percentage of attached cells decreased as the concentration of pine oil increased. The addition of pine oil was found to decrease the negative charge of chalcopyrite and cells, resulting in a higher attraction of acid-base interaction energy and total interaction force. This ultimately led to a stronger adhesion force between chalcopyrite and A. manzaensis. While the addition of pine oil initially delayed the formation of cell microcolonies during the first stage of biofilm formation, which ultimately resulted in larger biofilm coverage compared with the control group. The microcolonies on the mineral surface were also larger and the corrosion pits were bigger with the addition of pine oil. Furthermore, the addition of pine oil weakened the passivation effect and promoted the biological dissolution of chalcopyrite. This study offers a better understanding of the effect of pine oil on the bioleaching of chalcopyrite.
摘要：
Climate change has caused high salinity in many fields, particularly in the mud flats in coastal regions. The resulting salinity has become one of the most significant abiotic stresses affecting the world's rice crop productivity. Developing elite cultivars with novel salinity-tolerance traits is regarded as the most cost-effective and environmentally friendly approach for utilizing saline-alkali land. To develop a highly efficient green strategy and create novel rice germplasms for salt-tolerant rice breeding, this study aimed to improve rice salinity tolerance by combining targeted CRISPR/Cas9-mediated editing of the OsRR22 gene with heterosis utilization. The novel alleles of the genic male-sterility (GMS) and elite restorer line (733S(rr22)-T1447-1 and HZ(rr22)-T1349-3) produced 110 and 1 bp deletions at the third exon of OsRR22 and conferred a high level of salinity tolerance. Homozygous transgene-free progeny were identified via segregation in the T2 generation, with osrr22 showing similar agronomic performance to wild-type (733S and HZ). Furthermore, these two osrr22 lines were used to develop a new promising third-generation hybrid rice line with novel salinity tolerance. Overall, the results demonstrate that combining CRISPR/Cas9 targeted gene editing with the "third-generation hybrid rice system" approach allows for the efficient development of novel hybrid rice varieties that exhibit a high level of salinity tolerance, thereby ensuring improved cultivar stability and enhanced rice productivity.
通讯机构：
[Zhi Liu] C;[Junhua Chen] N;National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, 510650, China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, China
摘要：
We successfully constructed several cascaded molecular logic gates (2INHIBIT-2AND, 2AND-2OR, and 2OR-2INHIBIT) using three different antibiotics as the inputs. In the presence of kanamycin (KAN), chloramphenicol (CHL), or oxytetracycline (OXY), the aptamer-antibiotic recognition will release the trigger DNA to active the hairpin DNA hybridization. Exonuclease III (Exo III)-mediated catalysis reaction was introduced in the logic system to generate Mg2+-dependent DNAzyme, which was used to cleave the fluorescence signal reporter probe. For input, the presence and absence of the antibiotic was defined as 1 and 0, respectively. For output, the fluorescence intensity higher or lower than the threshold value was defined as 1 and 0, respectively. In the 2INHIBIT-2AND logic circuit, the 101 input combination generates an output of 1 and other input combinations generate an output of 0. In the 2AND-2OR logic circuit, the input combinations of 001, 011, 110, 101, and 111 generate an output of 1 and other input combinations generate an output of 0. In the 2OR-2INHIBIT logic circuit, the input combinations of 010, 100, and 110 generate an output of 1 and other input combinations generate an output of 0. Our constructed logic system exhibits high selectivity and can work even in complex water samples. With the advantages of multiple biocomputation capabilities, high flexibility, and easy scalability, this logic gate system provides a new analytical method for the intelligent detection of different antibiotics.
摘要：
Allelopathy has been considered a good explanation for the successful invasion of some invasive plants. However, the real latitudinal and longitudinal allelopathic effects on native species have rarely been documented since many exotics have spread widely. We conducted a Petri dish experiment to determine the latitudinal and longitudinal allelopathic patterns of an invasive alligator weed (Alternanthera philoxeroides) on a common crop (Lactuca sativa) in China, and find what determines the allelopathic intensity. The results showed that the allelopathic effects of A. philoxeroides increased with the latitude while decreased with the longitude. This indicated that A. philoxeroides used its allelopathy to gain competitive advantages more in its recent invaded communities than that in its early invaded ones as A. philoxeroides is expanding from southeast China to northwest China. Furthermore, we found that the allelopathic intensity of A. philoxeroide was negatively correlated to the leaf contents of soluble carbohydrate (SC), carbon (C) and nitrogen (N), but that was positively correlated to the leaf contents of soluble protein (SP), free amino acids (FAA), plant polyphenol (PP), phosphorus (P) and potassium (K). These results suggested that the allelopathic intensity of A. philoxeroide was more determined by the limited P and K nutrients as well as the intermediate allelochemicals (SP, FAA, PP) rather than the unlimited C, N and SC. Thus, we can speculate that the negative or positive effects of plant aqueous extracts are a function of not only the extract concentrations but also the trade-offs between inhibition and promotion of all components in the extracts. Then we could reduce the allelopathic effects of A. philoxeroide by controlling the component contents in the plant tissues, by fertilization or other managements, especially in the plant recent invaded communities.
