摘要：
Siraitia grosvenorii pomace (SGP) was processed by solid-state fermentation with Eurotium cristatum. Metabolite differences between non-fermented Siraitia grosvenorii pomace (NFSGP) and E. cristatum fermented Siraitia grosvenorii pomace (FSGP) were investigated by LC-MS/MS-based untargeted metabolomic analysis. The anti-oxidant and anti-aging effect of NFSGP and FSGP was investigated through in vitro and in vivo assays using Caenorhabditis elegans model. Results showed that 263 metabolites had significant changes after fermentation. Fermentation with E. cristatum significantly increase most polyphenols, flavonoids and alkaloids, such as sinapic acid, quercetin and indole. The antioxidant activities of SGP were also significantly enhanced after fermentation by E. cristatum. Furthermore, NFSGP and FSGP could significantly prolong the life-span and enhanced the oxidative and heat stress resistance of C. elegans, and FSGP exhibited greater effects. Our study revealed that fermentation with E. cristatum could improve the nutritional value of SGP.
作者机构：
[谢慧; 刘素纯] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410125, China;[武小芬; 陈亮; 王克勤; 邓明; 齐慧; 王振; 张祺玲; 刘安] Engineering Technology Research Center of Agricultural Biological Irradiation, Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agricultural Sciences, Changsha, 410125, China
通讯机构：
[Liu, S.] C;College of Food Science and Technology, China
摘要：
本试验利用电子束及γ射线两种射线对渐狭蜡蚧菌(Lecanicillium attenuatum)3166进行诱变处理, 研究适于渐狭蜡蚧菌诱变选育的吸收剂量以及诱变菌株生物学特性。结果表明: 电子束及γ射线诱变渐狭蜡蚧菌的最适剂量均为200 Gy, 渐狭蜡蚧菌的电子束辐射敏感性D10值为191 Gy、γ射线D10值为366 Gy; 以产孢速率为指标筛选获得突变菌株8株, 其中5株的菌丝生长及产孢速率较出发菌株显著提高, 均为电子束诱变所得, 产孢速率分别较出发菌株提高了30.45%、31.55%、23.66%、64.83%、68.77%; 诱变菌株的胞外几丁质酶比活在培养5 d时达到峰值, 诱变株Ⅱ111、Ⅱ164、Ⅱ181的几丁质酶比活分别较出发菌株显著提高29.29%、54.45%、19.18%, 其中Ⅱ164酶比活可达1 707.41 U/mg prot; 胞外蛋白酶比活在培养7 d时达到峰值, Ⅱ111、Ⅱ164、Ⅱ181的蛋白酶比活分别较出发菌株显著提高17.98%、13.17%、16.50%, 其中Ⅱ111酶比活达到30.71 U/mg prot, 且渐狭蜡蚧菌诱变株生长速率与胞外酶比活有强相关性。本研究为渐狭蜡蚧菌后续的害虫防治及开发利用提供了理论基础。 In this study, electron beam and γ-ray were used to mutagenize Lecanicillium attenuatum 3166, and the absorbed dose suitable for mutation breeding of Lecanicillium attenuatum and the biological characteristics of the mutagenized strain were studied. The results showed that the optimal dose for electron beam and γ-ray mutagenesis of Lecanicillium attenuatum was 200 Gy, the electron beam D10 value of Lecanicillium attenuatum was 191 Gy, and the γ-ray D10 value was 366 Gy; eight mutant strains were screened and obtained with spore production rate as the index. Among them, the hypha growth and spore production rate of five strains significantly increased compared with the parent strain, which were all obtained by electron beam mutagenesis. The spore production rate increased by 30.45%, 31.55%, 23.66%, 64.83% and 68.77% respectively compared with the parent strain. The extracellular chitinase activity of the mutant strain reached the peak after five days of culture, and the chitinase activities of mutant strains Ⅱ111, Ⅱ164 and Ⅱ181 increased by 29.29%, 54.45% and 19.18% respectively, compared with that of the parent strain. The chitinase activity of mutant strain Ⅱ164 reached 1 707.41 U/mg prot; the extracellular protease activity reached its peak after 7-day culture, and the protease activities of Ⅱ111, Ⅱ164 and Ⅱ181 were significantly increased by 17.98%, 13.17% and 16.50% respectively as compared with those of the parent strain, wherein the enzyme activity of Ⅱ111 could reach 30.71 U/mg prot, and the growth rate of the mutant strain had a strong correlation with the extracellular enzyme activity. This study provides a theoretical basis for the follow-up pest control, development and utilization of Lecanicillium attenuatum.
摘要：
TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR 1 (TCP) transcription factors control multiple aspects of growth and development in various plant species. However, few genes were reported to be directly targeted and regulated by them through their specific binding sites, and then uncover their functions in plants. A consensus DNA-binding site motif of TCP2 was identified by random binding site selection (RBSS). DNA recognized by TCP2 contained the motif G(G/T)GGNCC(A/C), which showed high consistency with motifs bound by other TCP domain proteins. Consequently, this motif was regarded as the specific DNA-binding sites of TCP2. Circadian clock associated 1 (CCA1) and EARLY FLOWERING 3 (ELF3) were subsequently considered as potential target genes owing to the containing of the similar TCP2 binding sites or core binding sites GGNCC and found to be positively regulated by TCP2 via DNA binding. Phenotype analysis results showed that mutation and over-expression of TCP2 resulted in variations in leaf morphogenesis, especially the double or triple mutations of TCP2, 4 and 10. Mutations in TCPs caused late flowering. Finally, TCP2 was shown to influence hypocotyl elongation by mediating the jasmonate signaling pathway. Overall, these results provide a basis for future studies aimed at distinguishing the target genes of TCP2 and elucidating the important roles of TCP2 in plant growth and development.
摘要：
选取茯砖茶中优势微生物冠突散囊菌,进行液体发酵培养,利用水提醇沉淀法提取发酵液中多糖.以苯酚-硫酸法测定总多糖含量,探寻操作过程中苯酚用量、硫酸用量以及显色时间对多糖含量测定的影响,并在单因素试验结果的基础上进行正交试验优化.研究结果表明,3个因素对冠突散囊菌发酵液多糖含量测定的影响程度依次为:浓硫酸用量>显色时间>苯酚用量,当5%苯酚用量为0.6 m L、浓硫酸用量为7.0 m L、显色时间为40 min时,可以获得苯酚-硫酸法对冠突散囊菌发酵液中多糖含量的最佳测定效果.
