通讯机构：
[He, J.; Wu, X.] H;Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, China
关键词：
gut microbiota;placental function;reproductive performance;resveratrol;sow
摘要：
<jats:p>Placental function is vital to the fetal growth of sows, and resveratrol (RES) can protect cells against oxidative stress, which is one of the major factors impairing placental function. This study aimed to investigate the effect of dietary resveratrol (RES) on placental function and reproductive performance during late pregnancy in a sow model from the aspects of oxidative stress, insulin resistance, and gut microbiota. A total of 26 hybrid pregnant sows (Landrace × Yorkshire) with similar parity were randomly allocated into two groups (<jats:italic>n</jats:italic> = 13) and fed with a basal diet or a diet containing 200 mg/kg of resveratrol from day 85 of gestation until parturition. The dietary supplementation of RES increased the litter weight at parturition by 12.53% (<jats:italic>p</jats:italic> = 0.145), with ameliorated insulin resistance (HOMA-IR), increased triglyceride (TG) levels, and decreased interleukin (IL)-1β and IL-6 levels in serum (<jats:italic>p</jats:italic> &lt; 0.05). Moreover, resveratrol increased the placental vascular density (<jats:italic>p</jats:italic> &lt; 0.05) with the enhanced expression of nutrient transporter genes (<jats:italic>SLC2A1</jats:italic> and <jats:italic>SLC2A3</jats:italic>) and antioxidant genes, such as <jats:italic>superoxide dismutase 2</jats:italic> (<jats:italic>SOD2</jats:italic>) and <jats:italic>heme oxygenase-1</jats:italic> (<jats:italic>HO-1</jats:italic>) but declined the expression of inflammatory genes, such as <jats:italic>IL-1</jats:italic>β and <jats:italic>IL-6</jats:italic> (<jats:italic>p</jats:italic> &lt; 0.05). The characterization of the fecal microbiota revealed that resveratrol decreased the relative abundance of the <jats:italic>Christensensllaceae R-7 group</jats:italic> and <jats:italic>Ruminococcaceae UCG-008</jats:italic> (<jats:italic>p</jats:italic> &lt; 0.05), which had a positive linear correlation with the expression of <jats:italic>IL-1</jats:italic>β and <jats:italic>IL-6</jats:italic> (<jats:italic>p</jats:italic> &lt; 0.05), but had a negative linear correlation with the expression of <jats:italic>SOD2, HO-1, SLC2A1</jats:italic>, and <jats:italic>SCL2A3</jats:italic> genes (<jats:italic>p</jats:italic> &lt; 0.05). These data demonstrated that dietary supplementation with resveratrol can improve placental function with ameliorated insulin resistance, oxidative stress, and inflammation potentially by regulating <jats:italic>Ruminococcaceae UCG-008</jats:italic> and the <jats:italic>Christensensllaceae R-7 group</jats:italic> in sows.</jats:p>
摘要：
<jats:title>Abstract</jats:title><jats:p>A total of twenty-four healthy twin-bearing Liuyang black goats were allocated to two trials. In Trial 1, twelve goats received either the control diet (CG, <jats:italic>n</jats:italic> 6, 100 % feed) or restricted diet (RG, <jats:italic>n</jats:italic> 6, 60 % feed of CG) from gestation days 26 to 65 after synchronisation. In Trial 2, the remaining goats were randomly and equally divided into two treatments: CG and RG from days 95 to 125 of gestation. Placental traits, fetal weight, serum parameters, nitric oxide (NO), angiogenesis gene expression and cotyledon proteome were measured at the end of each trial. In early pregnancy, the total and relative weights of placenta, uterine caruncle and cotyledon, as well as fetus, were increased (<jats:italic>P</jats:italic><0·05) in RG. The NO content in maternal serum was also increased (<jats:italic>P</jats:italic><0·05) in RG. In all, fifty differentially expressed proteins were identified in cotyledon. The up-regulated proteins are related to proliferation and fission of trophoblast cell and the placenta angiogenesis. During the late pregnancy trial, placental weight was increased (<jats:italic>P</jats:italic><0·05) in RG, but weight of the fetus was decreased (<jats:italic>P</jats:italic><0·05). The capillary density in the cotyledon was also decreased (<jats:italic>P</jats:italic><0·01). A total of fifty-eight proteins were differentially expressed in cotyledon. The up-regulated proteins in RG are related to placenta formation, blood flow regulation and embryonic development. These results indicated that feed intake restriction during gestation influenced the placental and fetal development in a stage-dependent manner. These findings have important implications for developing novel nutrient management strategies in goat production.</jats:p>
摘要：
Abstract: To investigate the protective effect of resveratrol (RES) on non-alcoholic fatty liver disease (NAFLD) in mice, in the present study, a NAFLD mouse model was established through feeding high-fat diet. Changes in body weight, liver histopathology, lipid composition of liver and serum, and seral cytokines were studied after interventive treatment with RES in the NAFLD mice by us-ing H.E. staining, colorimetric method and Luminex test for multi-factor. The results showed that after feeding high-fat diet, the mouse body weight was higher than that of the normal control group between 4~5 weeks (p ˂ 0.05); histopathological injury in liver tissue was obvious; lipid components in liver and serum including triglyceride (TG), total cholesterol (TC), low density li-poprotein cholesterol (LDL-C) levels were significantly increased, and the high density lipoprotein cholesterol (LDL-C) was significantly decreased; seral transaminases including ALT and AST, seral interleukin (IL)-2, IL-6, IL-1 beta and tumor necrosis factor (TNF-α) were also significantly increased. After intervention with RES, fatty liver lesions induced by high-fat diet were significantly alleviated; and levels of lipid components in liver and serum, seral transferase and cytokines were regulated by RES to some extent. All data indicate that RES has a preventive effect against the NAFLD in mice.#@#@#摘要: 本研究使用高脂日粮饲喂小鼠建立NAFLD动物模型，研究白藜芦醇(Resveratrol, RES)对非酒精性脂肪肝(Non-alcoholic fatty liver disease, NAFLD)的预防作用效果。采用H.E.染色、比色法及Luminex多因子检测等方法检测了RES干预处理后对体重、肝脏组织学变化、肝脂和血脂成分以及血清中细胞因子等的变化。结果显示：小鼠饲喂高脂日粮诱导后，体重在第4~5周时显著高于正常日粮饲喂对照组(p ˂ 0.05)；肝脏组织受到明显的病理学损伤；血脂和肝脂包括甘油三酯(Triglyceride, TG)、总胆固醇(Total cholesterol, TC)、低密度脂蛋白胆固醇(Low-density lipoprotein cholesterol, LDL-C)的水平均显著高于空白对照组，而高密度脂蛋白胆固醇(High-density lipoprotein cholesterol, LDL-C)则降低；血清转氨酶活力包括丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)、血清中细胞因子白介素(Interleukin, IL)-2、IL-6、IL-1β以及肿瘤坏死因子(Tumor necrosis factor alpha, TNF-α)的水平显著升高。用RES干预处理后，高脂所诱导的脂肪肝病变明显减轻，并在不同程度上对血脂和肝脂成分、血清转氨酶及部分细胞因子水平起到相应的调节作用。以上结果揭示，RES对小鼠NAFLD的发生有一定的预防作用。
