摘要：
Mycoplasma hyopneumoniae (M. hyopneumoniae) is the causative agent of swine enzootic pneumonia, resulting in substantial economic losses in global pig farming. Although vaccination is the primary strategy for controlling M. hyopneumoniae infection, current vaccines fall short in preventing transmission of this pathogen or protecting the body from secondary infection. This study aimed to assess the immunogenicity of six recombinant antigens (P97R1, P46, GAPDH, PdhA, DnaK, and EF-Tu) of M. hyopneumoniae through intramuscular immunization in mice. The results showed that the six antigens elicited high levels of serum IgG. Among them, P97R1, P46, PdhA, and DnaK stimulated robust antigen-specific IgA mucosal immune responses. CCK-8 assays revealed that both P97R1 and DnaK significantly increased the proliferation of mononuclear cells from spleen and lung, and DnaK also promoted the proliferation of blood mononuclear cells. Additionally, PdhA induced Th17-type immune response with a high level of IL-17 level in serum. Flow cytometry analysis indicated that P97R1 and PdhA increased the ratio of CD8+/CD4+ T lymphocyte, favoring cytotoxic T lymphocyte (CTL) immune responses. Notably, P97R1 immunization significantly decreased the percentages of CD4+ T cells while increased the percentages of CD8+ T cells. The present findings demonstrate that the candidate antigens P97R1, PdhA, and DnaK of M. hyopneumoniae induce specific humoral and mucosal immunity; P97R1 and DnaK also stimulated intense cellular immunity, and PdhA induced CTL and Th17-type immune responses. In conclusion, P97R1, PdhA, and DnaK emerge as potential candidate antigens for the future development of a more effective subunit vaccine against M. hyopneumoniae.
作者机构：
[Li, Zhiqiang; Deng, Zhibang; Yan, Kexin; Zheng, Fan; Cheng, Jiahao] Hunan Agr Univ HUNAU, Coll Vet Med, Hunan Prov Key Lab Prot Engn Anim Vaccines, Lab Anim Dis Prevent & Control & Anim Models, Changsha 410128, Hunan, Peoples R China.;[Feng, Simeng] Changsha Luye Biotechnol Co Ltd, Changsha 410100, Peoples R China.;[Yuan, Xiaomin] Hunan Agr Univ, Coll Vet Med, 1 Nongda Rd, Changsha 410128, Peoples R China.
通讯机构：
[Xiaomin Yuan] L;Lab of Animal Disease Prevention & Control and Animal Models, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), Changsha, Hunan 410128 China<&wdkj&>Changsha Luye Biotechnology Co., Ltd, Changsha 410100, China
关键词：
Antiviral infection;Interferon-induced transmembrane protein 3;Porcine coronavirus
摘要：
Interferon-induced transmembrane proteins (IFITMs) play an important role in the innate immune response triggered by viral infection. Transmissible gastroenteritis virus (TGEV) causes severe diarrhea, vomiting and dehydration in piglets, resulting in huge economic losses to the swine industry. In this study, we showed that IFITM3 inhibits the replication of TGEV and interferes with the binding of TGEV to PK15 cells. Moreover, the inhibitory effect of IFITM3 on TGEV circumvents the upregulation of inflammatory cytokines. Subsequently, we found that the M22A mutant loses part of the antiviral effect of IFITM3 on TGEV; in contrast, the K24A mutant enhances the antiviral effect of IFITM3. Notably, our data shows a synergistic effect between IFITM3 and CQ, which further amplifies the antiviral effect against TGEV.
期刊：
Frontiers in Veterinary Science,2022年9:1169 ISSN：2297-1769
作者机构：
[Nan, Wenjin; Deng, Zhibang; Tan, Simin] Hunan Agr Univ HUNAU, Coll Vet Med, Hunan Prov Key Lab Prot Engn Anim Vaccines, Lab Anim Dis Prevent & Control & Anim Model, Changsha, Peoples R China.;[Nan, Wenjin; Wu, Jingbo; Peng, Guoliang; Hu, Honghui] Shaoguan Univ, North Guangdong Collaborat Innovat & Dev Ctr Pig F, Shaoguan, Peoples R China.
关键词：
Porcine circovirus type 2;Epidemiology;complete genome;Genetic characteristics;Guangdong Provine
摘要：
The emergence and widespread of porcine circovirus-associated diseases (PCVADs), mainly caused by porcine circovirus type 2 (PCV2), threatens the Chinese swine industry. In this study, to investigate the recent prevalence of PCV2 in northern Guangdong Province of China, 573 tissue samples from 132 pig farms were collected during 2016-2021 and analyzed via PCR. Overall, 51.38% (297/573, 95%CI 47.74-55.92) samples were tested PCV2 positive. The detection rate of PCV2 was significantly lower in samples collected before 2016-2018 than after the outbreak of African Swine Fever (2019-2021), being 59.85% (158/264, 95%CI 53.94-65.76) and 41.47% (141/340, 95%CI 36.43-46.71), respectively. On the other end, the genetic characteristics of 26 PCV2 strains were further analyzed. These PCV2 strains belonged to three genotypes, including PCV2a, PCV2b, and PCV2d. Specifically, the predominant genotype prevalent during two periods (2016-2018 and 2019-2021) wasPCV2b (81.82%, 9/11) and PCV2d (80.0%, 12/15), respectively. The results above illustrated the high prevalence and the genetic evolution feature of PCV2 in Guangdong Province in recent years.
作者机构：
[Fang, Manxin; Hu, Wei] Yichun Univ, Coll Life Sci & Resource Environm, Yichun 336000, Jiangxi, Peoples R China.;[Deng, Zhibang] Res Ctr Reverse Vaccinol RCRV, Prov Key Lab Prot Engn Anim Vaccines, Changsha 410128, Hunan, Peoples R China.;[Deng, Zhibang] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote LFP, Changsha 410128, Hunan, Peoples R China.;[Deng, Zhibang] Hunan Agr Univ, Res Ctr Reverse Vaccinol RCRV, Prov Key Lab Prot Engn Anim Vaccines, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.;[Deng, Zhibang] Hunan Agr Univ, Coll Vet Med, Lab Funct Prote LFP, 1 Nongda Rd, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhibang Deng *] P;Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center of Reverse Vaccinology (RCRV), and Laboratory of Functional Proteomics (LFP), Hunan Agricultural University College of Veterinary Medicine, Changsha, China
作者：
Yuan, X. M.;Yuan, Q. C.;Feng, S. M.;Deng, Z. B.
期刊：
POLISH JOURNAL OF VETERINARY SCIENCES,2022年25(2):195-205 ISSN：1505-1773
通讯作者：
Deng, Z.B.
作者机构：
[Yuan, X. M.; Deng, Z. B.; Yuan, Q. C.; Feng, S. M.] Hunan Agr Univ, Coll Vet Med, Hunan Prov Key Lab Prot Engn Anim Vaccines, Lab Anim Dis Prevent & Control & Anim Model, 1 Nongda Rd, Changsha 410128, Peoples R China.
通讯机构：
Laboratory of Animal Disease Prevention and Control and Animal Model, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, No. 1 Nongda road, Furong District, Changsha, China
摘要：
Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVADs). The infection of PCV2 is widespread and has serious consequence, thereby causing significant economic losses in the swine industry worldwide. Previously, we found that a strain named YiY-3-2-3 has a naturally occurring point mutation (G710 to A710) in ORF1 region, which leads to a shorten product of the rep gene (945 to 660 base pair). Importantly, the Rep protein is responsible for genome replication of PCV2. To explore the effects of this mutation on the PCV2 replication, in the current study we constructed infectious clone of this IF-YiY-3-2-3, as well as those of its two parental strains of IF-YiY-3-2-1 and IF-YiY-3-2-10. Subsequently, these infectious clones which have 1.1 copy of PCV2 genome of their corresponding strains were transfected into PK15 cells to obtain rescued viruses, respectively. Though all of the three infectious clones could be rescued, the copy number and infectivity of these rescued viruses were significantly different, as analyzed by fluorescence quantitative PCR, Tissue culture infectious dose 50 (TCID50), and indirect immunofluorescence assay (IFA). Notably, whether the PCV2 copy number, viral titer or the infectivity of rescued viruses from infectious clone IF-YiY-3-2-3 was significantly less than those of its parental clones. Meanwhile, the spatial structure of the Rep protein from the IF-YiY-3-2-3 displayed an apparent truncation at the C-terminal. These findings therefore suggest that the Rep protein with truncated C-terminal would reduce virus replication and infectivity, and there might also exist both favorable and unfavorable mutations in the ORF1 of PCV2 in the process of its evolution.
摘要：
T-2 and HT-2 toxins can cause cytotoxicity and oxidative stress in animals, while DL-Selenomethionine plays an important role in preventing oxidative stress and improving cell viability. However, the role of DL-Selenomethionine in T-2/HT-2 toxins-induced cell damage is still unknown. In this study, we investigated whether DL-Selenomethionine plays a protective role against T-2/HT-2-induced cytotoxicity and oxidative stress in primary hepatocytes. Our results demonstrated that T-2/HT-2 toxins-exposed broiler hepatocytes exhibited significantly decreased cell viability and intracellular glutathione (GSH) concentration while increased Lacate dehydrogenase (LDH) leakage, intracellular reactive oxygen species (ROS), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and catalase (CAT) levels, as well as elevated expression levels of genes related to oxidative stress, in a toxin dose-dependent manner (P < 0.05). However, the application of DL-Selenomethionine into T-2/HT-2 treated hepatocytes effectively alleviated the adverse effects of T-2/HT-2, as demonstrated by increased cell viability, decreased LDH leakage, declined intracellular ROS and MDA levels, increased expression of oxidative stress-related genes, as well as accordingly enhanced activities of GSH, GSH-PX, SOD and CAT as compared to the control groups (P < 0.05). Therefore, our in vitro data demonstrate that DL-Selenomethionine can function as an effectively protective agent against T-2/HT-2-induced cytotoxicity and oxidative stress.
摘要：
Porcine circovirus type 3 (PCV3) has recently been isolated from diseased pigs within the USA. The objective was to detect the presence of PCV3 in dogs. Nested polymerase chain reactions (PCR) with PCV3-specific primers for the capsid gene were used to detect PCV3 genomic DNA in serum samples from dogs (n = 44) in China. There was PCV3 DNA detected in 4 of 44 dogs [all were negative for PCV2 and canine circovirus (CanineCV)]. Based on sequence analysis, positive sequences were grouped into PCV3 genotypes. However, these isolates had close evolutionary relationships with FoxCV (KP941114) and CanineCV (JQ821392). Further investigations of the epidemiology, evolutionary biology, and pathobiology of PCV3 to dogs are warranted.
期刊：
POLISH JOURNAL OF VETERINARY SCIENCES,2017年20(1):25-29 ISSN：1505-1773
通讯作者：
Deng, Z. B.
作者机构：
[Pang, P.; Yuan, A. W.; Deng, Z. B.; Gong, Q. L.] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Deng, Z. B.] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Life Sciences;Zoology;Life Sciences, other;Medicine;Veterinary Medicine
摘要：
Porcine circovirus type 2 (PCV2) has been known as a causative agent of reproductive failure in the sow. In the present study mouse model was used to investigate PCV2 infection. In order to investigate whether PCV2 can induce lesions of spermatocytes and oocytes, 6 male and 6 female mice were each inoculated intraperitoneally with PCV2b, and 3 male and 3 female mice mock-infected with cell culture supernatant served as controls. Samples of testes and ovaries from PCV2b-inoculated and mock-infected mice were investigated using PCR, histopathological, ultrastructural and immunofluorescent histochemical methods at 14 and 21 day post infection (dpi). The study revealed that in the virus-inoculated mice leydig cells in testes and granulosa cells in ovaries were degenerated, and a small number of spermatocytes and oocytes showed apoptosis. Positive PCV2b antigen signals were also observed in these apoptotic cells. It can be suggested that PCV2 can cause lesions of spermatocyte and oocyte prior to zygotes formation in its host.
