作者机构：
[洪忠举; 孙锦; 郭世荣; 魏斌; 王军伟; 王健; 李树海] College of Horticulture, Nanjing Agricultural University, Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology &, Equipment, Nanjing, 210095, China;[王军伟] College &, Landscape of Horticulture, Hunan Agricultural University, Changsha, 410128, China;[洪忠举; 孙锦; 郭世荣; 魏斌; 王军伟; 王健; 李树海] Nanjing Agricultural University (Suqian) Academy of Protected Horticulture, Suqian, 223800, China
通讯机构：
College of Horticulture, Nanjing Agricultural University, Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment, Nanjing, China
摘要：
In order to investigate the physiological functions and biosynthesis regulation of borneol or comphore in Artermisia annua L., which is the major source of the anti-malaria drug artemisinin, the full length cDNA of the gene encoding a borneol dehydrogenase (AaBDH) was cloned from A. annua for the first time by using RACE (rapid amplification of cDNA ends). The completed open read frame of AaBDH was 1415 bp and it encoded a 885-amino acid protein with a predicted molecular mass of 31.04 kDa and a pI of 6.16. AaBDH showed 68-70% of amino acid identity to alcohol dehydrogenases from Solanum lycopersicum, Ppulus trichocarpa, Morus notabili and Ricinus communis. While it shared 51% and 58% of identity with artemisia alcohol dehydrogenase ADH2 from A. annua and borneol dedrogenase LiBDH from Lavandula x intermedia, respectively. The recombinant protein was obtained by heterogeneous expression of AaBDH in a strain of Escherichia coli BL 21 and purified by affinity chromatography. The function of AaBDH was characterized by using of in vitro enzymatic assays, and the results showed that AaBDH had the ability to specifically convert borneol into camphor in the presence of NAD(+) (nicotinamide adenine dinucleotide) or NADP+ (nicotinamide adenine dinucleotide phosphate). The cloning of AaBDH laid a significant foundation for further investigation on physiological functions and biosynthesis regulation of plant monoterpenoids. (C) 2015 Elsevier B.V. All rights reserved.
作者机构：
[刘泽宇] School of Aeronautics, Northwestern Polytechnical University, Xi'an, China;[朱旗] Horticulture and Landscape College, Hunan Agricultural University, Changsha, China;[刘焱] Hunan Province Key Laboratory of Food Science and Biotechnology, Hunan Agricultural University, Changsha, China;[刘焱; 罗灿] College of Food Science and Technology, Hunan Agricultural University, Changsha, China
通讯机构：
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
作者机构：
[卲元元; 熊硕; 邓娣; 肖文军; 廖格] National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Provincial Co-Innovation Center of Ultilization of Botanical Function Ingredients, Hunan Agricultural University, Changsha, China;[廖格] College of Horticulture and Landscape, Hainan University, Haikou, China;[卲元元; 熊硕; 邓娣; 肖文军; 廖格] Botanical Resource Engineering Department of College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China
通讯机构：
National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Provincial Co-Innovation Center of Ultilization of Botanical Function Ingredients, Hunan Agricultural University, Changsha, China
期刊：
World Journal of Microbiology and Biotechnology,2015年31(8):1235-1240 ISSN：0959-3993
通讯作者：
Xiao, Hong-Bo
作者机构：
[Xiao, Hong-Bo; Wang, Ji-Ying] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.;[Sun, Zhi-Liang; Zhang, Da-Sheng] Hunan Agr Univ, Biol Vet Drugs Branch, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Xiao, Hong-Bo] H;Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
关键词：
Angiopoietin-like protein 2;Murine mastitis;Staphylococcus aureus
摘要：
Mastitis is the inflammation of the mammary gland. Recent research has shown that Angiopoietin-like protein 2 (ANGPTL2) is a key inflammatory mediator. In the present study, we tested whether there is a correlation between increased ANGPTL2 expression and inflammation in response to Staphylococcus aureus in murine mastitis and the mechanisms involved. Thirty mice were divided into two groups: blank control group, challenged group. The entire infused mammary glands were removed to observe the changes of histopathology, myeloperoxidase (MPO) activity, production of tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6, and genes expression of ANGPTL2, TNF-alpha and IL-6. In challenged group, the structure of mammary glands was damaged and the large areas of cell fragments were observed. The MPO activity, IL-6 and TNF-alpha concentrations, ANGPTL2, IL-6, and TNF-alpha mRNA levels were significantly elevated in challenged group compared with blank control group. The present findings indicate ANGPTL2 may mediate the inflammation in murine mastitis through the activation of IL-6 and TNF-alpha.
摘要：
Functional porous carbon (PC) derived from bio-friendly shaddock peel has been firstly explored as catalyst for vanadium redox flow battery (VRB). The prepared PC is micro-mesoporous with high BET surface area of 882.7 m(2) g(-1), has some surface oxygen-containing functional groups, and is doped with N and P heteroatoms. These three factors greatly favor the electrochemical reactions of VO2+/VO2+ on the PC modified glass carbon (PC-GC). Compared with the naked GC and graphite modified GC, the PC-GC presents a lower peak separation (66 mV), higher anodic current density (17.1 mA cm(-2)) and cathodic current density (15.0 mA cm(-2)). The VRB using PC modified graphite felt (GF) as positive electrode demonstrates an enhanced voltage efficiency of 82.7% at the current density of 60 mA cm(-2), and a better rate performance than that from the virginal GF. (C) 2015 Elsevier B.V. All rights reserved.
作者机构：
[吴琪; 陆英; 王振; 刘雪辉; 刘林峰] College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China;[陆英] National Research Center of Eng Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha 410128, China;[李佳银] Changsha Sunfull Bio-tech Co., Ltd., Changsha 410126, China
通讯机构：
College of Horticulture and Landscape, Hunan Agricultural University, China
关键词：
Stage extraction;Capsaicinoids;Red pigments;Carotenoids;Fresh red pepper (Capsicum)
摘要：
A method for stage extraction of capsaicinoids and red pigments from fresh red peppers (FRPs) was developed in this work. Firstly, capsaicinoids were extracted from FRPs without any drying process by 40%-50% ethanol. Then red colorants without piquancy were extracted from the residues after removal of capsaicinoids using 95% ethanol as solvent with the conditions of 90 degrees C of temperature of water bath, 4 mL/g of ratio of solvent to material and 120 min of extraction time. The yield of red pigments were 89.8% of total content of red colorants in FRPs, which is about 2.2 times that of dry red peppers (DRPs) dried from equivalent FRPs. Compared with conventional methods, the new processes were simple, but gave a high yield of red pigments without remain of toxic solvent. (C) 2014 Elsevier Ltd. All rights reserved.
通讯机构：
[Lin, Yong] H;Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
关键词：
Mass spectrometry;Membrane proteomics;Sample preparation;Shotgun analysis;Sodium deoxycholate
摘要：
In current shotgun-proteomics-based biological discovery, the identification of membrane proteins is a challenge. This is especially true for integral membrane proteins due to their highly hydrophobic nature and low abundance. Thus, much effort has been directed at sample preparation strategies such as use of detergents, chaotropes, and organic solvents. We previously described a sample preparation method for shotgun membrane proteomics, the sodium deoxycholate assisted method, which cleverly circumvents many of the challenges associated with traditional sample preparation methods. However, the method is associated with significant sample loss due to the slightly weaker extraction/solubilization ability of sodium deoxycholate when it is used at relatively low concentrations such as 1%. Hence, we present an enhanced sodium deoxycholate sample preparation strategy that first uses a high concentration of sodium deoxycholate (5%) to lyse membranes and extract/solubilize hydrophobic membrane proteins, and then dilutes the detergent to 1% for a more efficient digestion. We then applied the improved method to shotgun analysis of proteins from rat liver membrane enriched fraction. Compared with other representative sample preparation strategies including our previous sodium deoxycholate assisted method, the enhanced sodium deoxycholate method exhibited superior sensitivity, coverage, and reliability for the identification of membrane proteins particularly those with high hydrophobicity and/or multiple transmembrane domains.
作者机构：
[陆英; 李觅路; 刘雪辉; 陈惠衡] College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China;[陆英; 谭斌] Natl. Res. Ctr. of Engineering Technology For Utilization of Functional Ingredients From Botanicals, Changsha 410128, China
通讯机构：
College of Horticulture and Landscape, Hunan Agricultural University, China
摘要：
The Fusarium oxysporum species complex consists of fungal pathogens that cause serial vascular wilt disease on more than 100 cultivated species throughout the world. Gene function analysis is rapidly becoming more and more important as the whole-genome sequences of various F. oxysporum strains are being completed. Gene-disruption techniques are a common molecular tool for studying gene function, yet are often a limiting step in gene function identification. In this study we have developed a F. oxysporum high-efficiency gene-disruption strategy based on split-marker homologous recombination cassettes with dual selection and electroporation transformation. The method was efficiently used to delete three RNA-dependent RNA polymerase (RdRP) genes. The gene-disruption cassettes of three genes can be constructed simultaneously within a short time using this technique. The optimal condition for electroporation is 10 mu F capacitance, 300 Omega resistance, 4kV/cm field strength, with 1 mu g of DNA (gene-disruption cassettes). Under these optimal conditions, we were able to obtain 95 transformants per mu g DNA. And after positive-negative selection, the transformants were efficiently screened by PCR, screening efficiency averaged 85%: 90% (RdRP(1)), 85% (RdRP(2)) and 77% (RdRP(3)). This gene-disruption strategy should pave the way for high throughout genetic analysis in F. oxysporum. (C) 2014 Elsevier GmbH. All rights reserved.
期刊：
JOURNAL OF ORGANIC CHEMISTRY,2014年79(22):11264-11269 ISSN：0022-3263
通讯作者：
Liang, Yun
作者机构：
[Huang, Hui; Wu, Lijun; Liang, Yun] Hunan Normal Univ, Natl & Local Joint Engn Lab New Petrochem Mat & F, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Hunan, Peoples R China.;[Cheng, Pi] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liang, Yun] H;Hunan Normal Univ, Natl & Local Joint Engn Lab New Petrochem Mat & F, Key Lab Chem Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Hunan, Peoples R China.
摘要：
A metal-free decarboxylative amination of 4-(ethoxycarbonyl)-2,3-allenols by TsNCO via base-induced aza-Michael addition/elimination has been developed. A variety of substituted N-tosyl 1,3-dien-2-yl amines were obtained in good yields and excellent regio- and stereoselectivity. Moreover, this transformation could be applied in preparation of 2-amino-trienes.
摘要：
This study is an assessment of an improved temperature warming system developed to enhance global warming research-based forest ecosystem and soil ecophysiological experiments. The architecture couples a standard open-top chamber (OTC) with a heating cable. A 16 m wire cable with an 18 W m<sup>-1</sup> and 288 W h<sup>-1</sup> power rating was coiled around a polyvinyl chloride (PVC) pipe 2.5 m in length and 3.5 cm in diameter. The pipe was reshaped into a circle and fixed inside the OTC at a height of 15 cm. PVC pipe distance to plants was 10 to 15 cm while distance to OTC inner walls was 15 cm. The cable was constructed from a heating source with an alloy resistance wire, an aluminum foil and copper wire shielded layer, a crosslinking polyethylene inner insulator, a PVC coating, and a tinned copper grounding wire. After the cable is powered up, air and soil inside the OTC-cable system is heated by conductivity. Temperature is manipulated according to the voltage and resistance of the cable. The OTC-cable system was developed to examine plant reaction to an increase in air and soil temperatures by 2.84 °C and 1.83 °C, respectively. Temperature values are adjustable by changing cable and PVC pipe length. It offers a new, affordable, low energy consumption and low running cost method by which to study climate change effects on forest ecosystems. This method is especially useful for application in forest ecosystems of many developing countries or in many remote areas of developed countries where the feasibility in supplying sufficient power from local power grids is questionable.
