摘要：
The chemical structure of sinoacutine is formed by a phenanthrene nucleus and an ethylamine bridge. Because it has a similar parent structure to morphine, it is subdivided into morphinane. At present, all reports have pointed out that the basic skeleton of morphine alkaloids is salutaridine (the isomer of sinoacutine), which is generated by the phenol coupling reaction of (R)-reticuline. This study shows that the biosynthetic precursors of sinoacutine and salutaridine are different. In this paper, the sinoacutine synthetase (SinSyn) gene was cloned from Sinomenium acutum and expressed SinSyn protein. Sinoacutine was produced by SinSyn catalyzed (S)-reticuline, according to the results of enzyme-catalyzed experiments. The optical activity, nuclear magnetic resonance, and mass spectrum of sinoacutine and salutaridine were analyzed. The classification and pharmacological action of isoquinoline alkaloids were discussed. It was suggested that sinoacutine should be separated from morphinane and classified as sinomenine alkaloids.
作者机构：
[Gen-Cai Song] Hunan Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;Authors to whom correspondence should be addressed.;College of Agronomy, Hunan Agricultural University, Changsha 410128, China;These authors contributed equally to this work.;[Jia-Qi Zhou; Jing Zhao] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
通讯机构：
[Feng-Lin Huang] H;[Shuan Meng] A;Authors to whom correspondence should be addressed.<&wdkj&>College of Agronomy, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China<&wdkj&>Authors to whom correspondence should be addressed.
摘要：
Nitrogen and brassinosteroids (BRs) play a vital role in modulating the growth, development, and yield of rice. However, the influences of BRs on nitrogen assimilation and metabolism in rice are not fully understood. In this study, we analyzed the impact of BRs on nitrogen utilization in rice using the indica variety ‘Zhongjiazao 17’ and the japonica variety ‘Nipponbare’ in hydroponic conditions. The results showed that BR treatment could efficiently elevate nitrate and ammonium nitrogen accumulation in both shoots and roots. Furthermore, some genes involved in the uptake of nitrate and ammonium in roots were stimulated by BRs, though we noted subtle variances between the two rice cultivars. Moreover, BRs augmented the activity of nitrate reductase (NR) and glutamine synthetase (GS) in roots, along with NR in shoots. Interestingly, BRs also spiked the total free amino acid content in both the shoots and roots. Gene expression analysis uncovered a robust induction by BRs of NR genes and GS-related genes in the roots of both ‘Nipponbare’ and ‘Zhongjiazao 17’. Collectively, our data suggest that BRs significantly enhance the accumulation of both nitrate and ammonium in rice and trigger a series of reactions related to nitrogen utilization.
作者机构：
[Xiao Li; Duo Zheng; Qiqian Wu; Hua Liu; Yan Li; Xinping Wang; Yanhong Wang] State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China;[Naili Zhang] State Key Laboratory of Efficient Production of Forest Resources and the Key Laboratory of Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing, China;[Lijia Dong] School of Life and Environmental Sciences, Shaoxing University, Shaoxing, China;[Aiping Wu] Ecology Department, College of Environment and Ecology, Hunan Provincial Key Laboratory of Rural Ecosystem Health in Dongting Lake Area, Hunan Agricultural University, Changsha, China;[Mingshui Zhao] Zhejiang Tianmu Mountain National Nature Reserve Administration, Hangzhou, China
通讯机构：
[Xinping Wang; Yanhong Wang] S;State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China<&wdkj&>State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China
摘要：
Salt spray is a natural disturbance in coastal region. Arbuscular mycorrhizal fungi (AMF) are recognized as bio-ameliorators of soil salinity in plants. However, the mechanism through which AMF protects Cinnamomum camphora against aerial salinity remains unclear. To address this knowledge gap, plants were subjected to four fungal regimes, namely sterilized fungal inoculum, Glomus tortuosum, Funneliformis mosseae, or a combination of these two fungi, and exposed to three sprayed-salt regimes (0, 7, or 14 mg NaCl cm−2 d−1) in a greenhouse. Salt spray significantly decreased photosynthetic capabilities, total dry weight, and salinity tolerance of non-mycorrhizal plants. Mycorrhizal inoculation, particularly a combination of G. tortuosum and F. mosseae, evidently mitigated the detrimental effects induced by salt spray. Meanwhile, mycorrhiza-mediated protection depended on the intensity of sprayed salt and the identity of fungal taxa. Furthermore, the enhanced resistance of mycorrhizal C. camphora seedlings to aerial salinity was mainly owing to increased leaf thickness and photosynthetic capabilities. These findings imply that inoculation with combined fungi could be an optimal strategy for cultivating C. camphora plants in coastal regions. The results gained hold the potential to offer both theoretical and practical guidance for the managers of coastal ecosystems in soil restoration and conservation.
通讯机构：
[Xue, S ] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
关键词：
Perennial energy crops;Root traits;Soil organic carbon;Microbial necromass;C 4-derived carbon
摘要：
The sustainability of bioenergy cropping systems hinges on the dynamics of soil organic carbon (SOC). Switchgrass and Miscanthus, as the two leading perennial energy crops, have been extensively cultivated on marginal land for bioenergy production. However, the effects of their cultivation on SOC sequestration and its underlying mechanisms remain unclear. Herein, we quantified the contributions of plant– and microbial–derived C to SOC accumulation by tracing 13C natural abundance and amino sugars on switchgrass- and Miscanthus-planted lands (i.e. belongs to poor acidic red soil) experienced 10 years of C3–C4 vegetation conversion. The results showed Miscanthus cultivation induced an approximately 6.3 times greater improvement in SOC compared to switchgrass. However, the organic C stability in Miscanthus-planted soil was comparatively lower than that of switchgrass. This was consistent with our global meta-analysis, whereby Miscanthus and switchgrass cultivation were observed to increase SOC by 16.0% and 7.1%, respectively. Miscanthus–cultivated soil was more replenished by plant–derived C stored in particulate organic C, owing to the greater biomass and lower root quality (reflected by the high ratio of lignin to nitrogen). In contrast, switchgrass–cultivated soil was enriched with more microbial–derived C, as its greater root quality induced a more efficient C utilization by the microbes. This was preferentially associated with the soil minerals. In conclusion, perennial energy crops cultivation on marginal land substantially enhances SOC sequestration, whereas the stability of SOC is dependent on the root traits.
摘要：
BACKGROUND: Salted hen egg yolks are less oily and less flavorful than salted duck egg yolks. However, hen eggs have a more adequate market supply and have a broader application prospect than duck eggs. In this study, egg yolks, plasma, and granules were dehydrated by adding 1% NaCl to simulate traditional curing process of salted egg yolk. The changes in the pickling process of hen egg yolks (HEY) and duck egg yolks (DEY) plasma, and granules were compared to reveal the gelation mechanism and the underlying causes of quality differences in salted HEY and DEY. Salted HEY can be compared with the changes in DEY during the pickling process to provide a theoretical basis for the quality improvement of salted HEY to salted DEY. RESULTS: The results showed that both plasma and granules were involved in gel formation, but exhibited different aggregation behaviors. Based on the intermolecular forces, the HEY proteins achieved aggregation mainly through hydrophobic interactions and DEY proteins mainly through covalent binding. According to spin-spin relaxation time, HEY gels immobilized a large amount of lipid and interacted strongly with lipids. DEY gels showed much free lipid and had weak interaction with lipid. The microstructure showed that HEY proteins were easily unfolded to form a homogeneous three-dimensional gel network structure after salting, while heterogeneous aggregates were formed to hinder the gel development in DEY. Changes in protein secondary structure content showed that pickling can promote the transformation of the α-helices to β-sheets structure in HEY gels, while more α-helices structure was formed in DEY gels. CONCLUSION: The study demonstrated that different gelation behaviors of hen and duck egg yolk proteins (especially in plasma) through salting treatment led to the difference in the quality of salted HEY and DEY. This article is protected by copyright. All rights reserved.
期刊：
Drug Design, Development and Therapy,2024年18:699-717 ISSN：1177-8881
通讯作者：
Chun Guo
作者机构：
[Yang, Yi; Lei, Shihui] Experiment Center of Medical Innovation, the First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China;The First Clinical College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China;[Li, Jiaqi; He, Ying; Yang, Hua] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, 410128, People’s Republic of China;[Zhang, Mengxue; Zhou, Desheng] Department of Neurology, the First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China;[Zhou, Xuqing; Wang, Xu; Guo, Chun] Experiment Center of Medical Innovation, the First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China<&wdkj&>The First Clinical College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China
通讯机构：
[Chun Guo] E;Experiment Center of Medical Innovation, the First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China<&wdkj&>The First Clinical College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People’s Republic of China
摘要：
BACKGROUND: Annao Pingchong decoction (ANPCD) is a traditional Chinese decoction which has definite effects on treating intracerebral hemorrhage (ICH) validated through clinical and experimental studies. However, the impact of ANPCD on oxidative stress (OS) after ICH remains unclear and is worth further investigating. AIM: To investigate whether the therapeutic effects of ANPCD on ICH are related to alleviating OS damage and seek potential targets for its antioxidant effects. MATERIALS AND METHODS: The therapeutic candidate genes of ANPCD on ICH were identified through a comparison of the target genes of ANPCD, target genes of ICH and differentially expressed genes (DEGs). Protein-protein interaction (PPI) network analysis and functional enrichment analysis were combined with targets-related literature to select suitable antioxidant targets. The affinity between ANPCD and the selected target was verified using macromolecular docking. Subsequently, the effects of ANPCD on OS and the selected target were further investigated through in vivo experiments. RESULTS: Forty-eight candidate genes were screened, in which silent information regulator sirtuin 1 (SIRT1) is one of the core genes that has antioxidant effects and ICH significantly affected its expression. The good affinity between 6 compounds of ANPCD and SIRT1 was also demonstrated by macromolecular docking. The results of in vivo experiments demonstrated that ANPCD significantly decreased modified neurological severity scoring (mNSS) scores and serum MDA and 8-OHdG content in ICH rats, while significantly increasing serum SOD and CAT activity, complicated with the up-regulation of ANPCD on SIRT1, FOXO1, PGC-1α and Nrf2. Furthermore, ANPCD significantly decreased the apoptosis rate and the expression of apoptosis-related proteins (P53, cytochrome c and caspase-3). CONCLUSION: ANPCD alleviates OS damage and apoptosis after ICH in rats. As a potential therapeutic target, SIRT1 can be effectively regulated by ANPCD, as are its downstream proteins.
摘要：
Ulcerative colitis (UC) is a chronic, non-specific inflammatory sickness of the intestinal tract, chiefly implicating the rectum and colon, which is characterized by chronic or subacute diarrhea, mucopurulent stools, and abdominal pain. The pathogeny of UC is still uncertain, and it is thought that multiple factors interact to cause the disease, such as environment, genetics, gut microbes, and immunity. Injuring the intestinal barrier is one of the most significant features of UC and includes mechanical, chemical, immune, and biological barriers. Plenty of research has shown that probiotics, as profitable bacteria in the gut, can play a prominent role in the treatment of UC by improving gut barrier function and modulating gut immunity. Lactobacillus plantarum (L. plantarum), a common probiotic, has made outstanding contributions to food and medicine, and many studies in recent years have shown that L. plantarum has great preventive and therapeutic effects on ulcerative colitis and restores the intestinal barrier. This paper reviews the mechanisms of L. plantarum for improving the intestinal barrier function of UC organisms, mainly including regulating the immune response, inhibiting oxidative stress, raising the expression of tight junction (TJ) proteins, promoting the formation of mucin, improving the composition of gut flora, and raising the levels of short-chain fatty acids (SCFAs), which offers some help for the clinical therapy of UC.
作者机构：
[Xiang, Sha; Ma, Jingjing; Liu, Xianjing; Luo, Yuan; Chen, Liang; Wang, Ying; Li, Zhihuan; Duan, Shuyang] College of Bioscience and Biotechnology, Hunan Agricultural University, China;[Jiang, Lihong] College of Resources, Hunan Agricultural University, China;[Xiao, Yunhua] College of Bioscience and Biotechnology, Hunan Agricultural University, China. Electronic address: xyh2017@hunau.edu.cn
通讯机构：
[Yunhua Xiao] C;College of Bioscience and Biotechnology, Hunan Agricultural University, China
关键词：
Cd uptake and efflux;Gene prediction;Microbial community;Migration and transformation
摘要：
Cadmium (Cd)-contaminated soil poses a severe threat to crop production and human health, while also resulting in a waste of land resources. In this study, two types of organic fertilizer (ZCK: Low-content available iron; Z2: High-content available iron) were applied to Cd-contaminated soil for rice cultivation, and the effects of the fertilizer on rice growth and Cd passivation were investigated in conjunction with soil microbial analysis. Results showed that Z2 could alter the composition, structure, and diversity of microbial communities, as well as enhance the complexity and stability of the microbial network. Both 2% and 5% Z2 significantly increased the fresh weight and dry weight of rice plants while suppressing Cd absorption. The 2% Z2 exhibited the best Cd passivation effect. Gene predictions suggested that Z2 may promote plant growth by regulating microbial production of organic acids that dissolve phosphorus and potassium. Furthermore, it is suggested that Z2 may facilitate the absorption and immobilization of soil cadmium through the regulation of microbial cadmium efflux and uptake systems, as well as via the secretion of extracellular polysaccharides. In summary, Z2 can promote rice growth, suppress Cd absorption by rice, and passivate soil Cd by regulating soil microbial communities.
作者机构：
[Gao, Yu-Hao; Di, Shu-Ke; Ma, Jing-Xian; Shao, Chang-Yan; Bai, Qin-Ru; Bian, Yu-Jing; Wang, Wen-Hui; Lei, Yun-Tao; Huang, Yi-Zhe] Schools of Basic Medicine and Clinical Pharmacy and Traditional Chinese Pharmacy, and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China;[Yang, Hui] Tea Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan, China;[Yang, Xiao-Na] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Cao, Peng] Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China;[Li, Chang-Zhu] State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, Hunan, China
通讯机构：
[Yu, Ye; Sun, Meng-Yang] S;Schools of Basic Medicine and Clinical Pharmacy and Traditional Chinese Pharmacy, and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address:
关键词：
S4-S5 linker domain;TRP-box domain;TRPV1 analgesics;biased allosteric mechanism;core body temperature;hyperthermia;nociception
摘要：
Efforts on developing transient receptor potential vanilloid 1 (TRPV1) drugs for pain management have been hampered by deleterious hypo- or hyperthermia caused by TRPV1 agonists/antagonists. Here, we compared the effects of four antagonists on TRPV1 polymodal gating and core body temperature (CBT) in Trpv1(+/+), Trpv1(-/-), and Trpv1(T634A/T634A). Neither the effect on proton gating nor drug administration route, hair coverage, CBT rhythmic fluctuations, or inflammation had any influence on the differential actions of TRPV1 drugs on CBT. We identified the S4-S5 linker region exposed to the vanilloid pocket of TRPV1 to be critical for hyperthermia associated with certain TRPV1 antagonists. PSFL2874, a TRPV1 antagonist we discovered, is effective against inflammatory pain but devoid of binding to the S4-S5 linker and inducing CBT changes. These findings implicate that biased allosteric mechanisms exist for TRPV1 coupling to nociception and CBT regulation, opening avenues for the development of non-opioid analgesics without affecting CBT.
关键词：
Spent mushroom compost;Nitrogen loss;Microbial community structure;Core microorganisms;Nitrogen metabolism;Chicken manure
摘要：
Due to the rapid development of animal husbandry, the associated environmental problems cannot be ignored, with the management of livestock and poultry manure emerging as the most prominent issue. Composting technology has been widely used in livestock and poultry manure management. A deeper understanding of the nitrogen conversion process during composting offers a theoretical foundation for selecting compost substrates. In this study, the effects of sawdust (CK) and spent mushroom compost (T1) as auxiliary materials on nitrogen as well as microbial structure in the composting process when composted with chicken manure were investigated. At the end of composting, the nitrogen loss of T1 was reduced by 17.18% relative to CK. When used as a compost substrate, spent mushroom compost accelerates the succession of microbial communities within the compost pile and alters the core microbial communities within the microbial community. Bacterial genera capable of cellulose degradation (Fibrobacter, Herbinix) are new core microorganisms that influence the assimilation of nitrate reduction during compost maturation. Using spent mushroom compost as a composting substrate increased the enzyme activity of nitrogen assimilation while decreasing the enzyme activity of the denitrification pathway.
摘要：
Squalene is an important triterpene with a wide range of applications. Given the growing market demand for squalene, the development of microbial cell factories capable of squalene production is considered a sustainable method. This study aimed to investigate the squalene production potential of Yarrowia lipolytica. First, HMG-CoA reductase from Saccharomyces cerevisiae and squalene synthase from Y. lipolytica was co-overexpressed in Y. lipolytica. Second, by enhancing the supply of NADPH in the squalene synthesis pathway, the production of squalene in Y. lipolytica was effectively increased. Furthermore, by constructing an isoprenol utilization pathway and overexpressing YlDGA1, the strain YLSQ9, capable of producing 868.1 mg/L squalene, was obtained. Finally, by optimizing the fermentation conditions, the highest squalene concentration of 1628.2 mg/L (81.0 mg/g DCW) in Y. lipolytica to date was achieved. This study demonstrated the potential for achieving high squalene production using Y. lipolytica.
关键词：
Cd fractionation;Microbial community;Migration and transformation
摘要：
The issue of heavy metal pollution caused by human production and living activities is progressively worsening. This study explored the effect of iron-rich organic fertilizer on the growth, quality, and cadmium (Cd) absorption of Chinese cabbage under Cd stress. The results showed that iron-rich organic fertilizer could increase the soluble protein content and root length of Chinese cabbage. Meanwhile, it could change the form of Cd to inhibit the enrichment of Cd in Chinese cabbage. The alkali hydrolyzed nitrogen (AN), total potassium (TK), organic matter (OM), and moisture content (MC) of the Z3 treatment group (2% ferrous sulfate heptahydrate) were significantly higher than those of other treatment groups. The microbial network of Z3 was more complex than the other three groups. PICRUSt analysis and correlation analysis showed that the genes related to protein synthesis (e.g., glutathione S-transferase, zinc and Cd transporter, outer membrane protein, ArsR family transcriptional regulator, catalase, etc.) can also promote microbial absorption. This study aims to provide theoretical insights into soil Cd pollution immobilization techniques.
摘要：
Osteoporosis (OP) is a skeletal disorder characterized by decreased bone density, alterations in bone microstructure, and increased damage to the bones. As the population ages and life expectancy increases, OP has become a global epidemic, drawing attention from scientists and doctors. Because of polyphenols have favorable antioxidant and anti-allergy effects, which are regarded as potential methods to prevent angiocardipathy and OP. Polyphenols offer a promising approach to preventing and treating OP by affecting bone metabolism, reducing bone resolution, maintaining bone density, and lowering the differentiation level of osteoclasts (OC). There are multiple ways in which polyphenols affect bone metabolism. This article provides an overview of how polyphenols inhibit oxidative stress, exert antibacterial effects, and prevent the occurrence of OP. Furthermore, we will explore the regulatory mechanisms and signaling pathways implicated in this process. (c) 2023 Elsevier Inc. All rights reserved.
摘要：
Phycocyanobilin (PCB) is a blue pigment with antioxidant, anti-inflammatory, and anticancer properties. It is used in the medical and cosmetic industries. In this study, a high-expression plasmid, pET-30a-PCB, was constructed for expression of PCB in Escherichia coli BL21(DE3). The PCB was analyzed using UV-visible absorption spectrum, MALDI-TOF-MS, and fluorescence spectra. The stability and half-life of PCB in different serum were determined. The yield of PCB was optimized through single-factor and orthogonal experiments. The optimal expression conditions were determined as a lactose concentration of 5 mmol/L, an induction time of 8 h, an induction temperature of 27 degrees C, and an induction duration of 22 h. PCB yield of 6.5 mg/L was achieved and subsequently purified using nickel-affinity chromatography. The purified PCB was quantified indirectly using Hist-tag ELISA detection, and the concentration was 11.66 mu g/L. In the range of 0-33 mu g/mL, the total antioxidant capacity and reducing the capacity of PCB were stronger than Vitamin E (Ve), with 1,1-diphenyl-2-picrylhydrazil (DPPH) scavenging reaching up to 87.07%, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) free radical (ABTS) scavenging up to 100%, hydroxyl radicals (<middle dot>OH) scavenging up to 64.19%, hydrogen peroxide (H2O2) scavenging up to 78.75%, This study provides theoretical evidence for PCB as a potent antioxidant.
摘要：
The ADP-ribosylation factor 6 (Arf6), as the only member of the Arf family III protein, has been extensively studied for its diverse biological functions in animals. Previously, the Arf6 protein in Magnaporthe oryzae was found to be crucial for endocytosis and polarity establishment during asexual development. However, its role remains unclear in S. sclerotiorum. Here, we identified and characterized SsArf6 in S. sclerotiorum using a reverse genetic approach. Deletion of SsArf6 impaired hyphal growth and development and produced more branches. Interestingly, knockout of SsArf6 resulted in an augmented tolerance of S. sclerotiorum towards oxidative stress, and increased its sensitivity towards osmotic stress, indicative of the different roles of SsArf6 in various stress responses. Simultaneously, SsArf6 deletion led to an elevation in melanin accumulation. Moreover, the appressorium formation was severely impaired, and fungal virulence to host plants was significantly reduced. Overall, our findings demonstrate the essential role of SsArf6 in hyphal development, stress responses, appressorium formation, and fungal virulence to host plants.
摘要：
Allelopathy has been considered a good explanation for the successful invasion of some invasive plants. However, the real latitudinal and longitudinal allelopathic effects on native species have rarely been documented since many exotics have spread widely. We conducted a Petri dish experiment to determine the latitudinal and longitudinal allelopathic patterns of an invasive alligator weed (Alternanthera philoxeroides) on a common crop (Lactuca sativa) in China, and find what determines the allelopathic intensity. The results showed that the allelopathic effects of A. philoxeroides increased with the latitude while decreased with the longitude. This indicated that A. philoxeroides used its allelopathy to gain competitive advantages more in its recent invaded communities than that in its early invaded ones as A. philoxeroides is expanding from southeast China to northwest China. Furthermore, we found that the allelopathic intensity of A. philoxeroide was negatively correlated to the leaf contents of soluble carbohydrate (SC), carbon (C) and nitrogen (N), but that was positively correlated to the leaf contents of soluble protein (SP), free amino acids (FAA), plant polyphenol (PP), phosphorus (P) and potassium (K). These results suggested that the allelopathic intensity of A. philoxeroide was more determined by the limited P and K nutrients as well as the intermediate allelochemicals (SP, FAA, PP) rather than the unlimited C, N and SC. Thus, we can speculate that the negative or positive effects of plant aqueous extracts are a function of not only the extract concentrations but also the trade-offs between inhibition and promotion of all components in the extracts. Then we could reduce the allelopathic effects of A. philoxeroide by controlling the component contents in the plant tissues, by fertilization or other managements, especially in the plant recent invaded communities.
作者：
Joaquín Castro-Montoya;Yasir Iqbal;Moritz von Cossel*
期刊：
Agronomy,2023年13(11):2818- ISSN：2073-4395
通讯作者：
Moritz von Cossel
作者机构：
Biobased Resources in the Bioeconomy (340b), Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Germany;Author to whom correspondence should be addressed.;[Yasir Iqbal] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Joaquín Castro-Montoya] Institute of Agri-Food and Environmental Sciences, Faculty of Agricultural Sciences, University of El Salvador, San Salvador 01101, El Salvador;[Moritz von Cossel] Biobased Resources in the Bioeconomy (340b), Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Germany<&wdkj&>Author to whom correspondence should be addressed.
通讯机构：
[Moritz von Cossel] B;Biobased Resources in the Bioeconomy (340b), Institute of Crop Science, University of Hohenheim, 70599 Stuttgart, Germany<&wdkj&>Author to whom correspondence should be addressed.
摘要：
Planet Earth is facing numerous imminent challenges, from climate change to ecological dysfunction, which are largely attributed to anthropogenic activities [...]
摘要：
Cordycepin, a nucleoside compound with a variety of biological activities, has been extensively applied in the nutraceutical and pharmaceutical industries. The advancement of microbial cell factories using agro-industrial residues provides a sustainable pathway for cordycepin biosynthesis. Herein, the cordycepin production was enhanced by the modification of glycolysis and pentose phosphate pathway in engineered Yarrowia lipolytica. Then, cordycepin production based on economical and renewable substrates (sugarcane molasses, waste spent yeast, and diammonium hydrogen phosphate) was analyzed. Furthermore, the effects of C/N molar ratio and initial pH on cordycepin production were evaluated. Results indicated that the maximum cordycepin productivity of 656.27mg/L/d (72h) and cordycepin titer was 2286.04mg/L (120h) by engineered Y. lipolytica in the optimized medium, respectively. The cordycepin productivity in the optimized medium was increased by 28.81% compared with the original medium. This research establishes a promising way for efficient cordycepin production from agro-industrial residues.
