摘要：
Bopu powder (R) and Sangrovit (R) were developed from Macleaya cordata and are widely used in agriculture and animal husbandry, but their impurities have been rarely reported in the literature. Impurity analysis is of great importance to the quality and safety of veterinary drugs. In this study, high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening method was used to screen and characterize the impurities in Bopu powder (R) and Sangrovit (R). A total of 58 impurities were screened from Bopu powder (R) and Sangrovit (R) using the screening strategies, of which 39 were identified by their accurate m/z value, characteristic MS/MS data, and fragmentation pathways of references. This established method was used for impurity analysis for the first time and proved to be a useful and rapid tool to screen and identify the impurities of Bopu powder (R) and Sangrovit (R), especially for those at trace levels in a complex sample. In addition, this study marks the first comprehensive research into impurities in these two products and has great significance for the systematic detection of impurities in other plant-derived drugs.
摘要：
Hemerocallis citrina Borani is an important crop and its flower buds are widely consumed in East Asian areas as a vegetable, as well as in traditional Chinese medicine, due to its health-promoting properties. Metabolites present in plant-derived foods or medicines are in part responsible for their desirable flavor profiles and health benefits. Nevertheless, detailed information about these compounds in H. citrina is scarce. Therefore, this study aimed to investigate the metabolites by high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). In this study, a total of 144 compounds, including 14 amides, 25 polyphenols, 44 flavonols, 35 anthraquinones, 15 naphthols, and 11 other components, were detected by the established screening method and were identified by their precise m/z values, characteristic tandem mass spectrometry (MS/MS) data and fragmentation pathways of references, 111 of which were reported in this plant for the first time. The distribution of identified ingredients in different parts of H. citrina was determined. Interestingly, colchicine, which had been reported as a toxic compound in the fresh flower buds in previous studies and various news reports, was not found. This work marks the first comprehensive study of metabolites from commercial flower buds and different parts of H. citrina. (C) 2020 Elsevier B.V. All rights reserved.
摘要：
Four novel acylglycosides flavones (AGFs) including two quercetin acylglycosides and two kaempferol acylglycosides were isolated from Fuzhuan brick tea (FBT) as follows: quercetin 3-O-[alpha-l-rhamnopyranosyl (1-->3)] [2-O''-(E)-p-coumaroyl] [beta-d-glucopyranosyl (1-->3)-alpha-l-rhamnopyranosyl (1-->6)]-beta-d-galactoside was named as camelliquercetiside E (1), quercetin 3-O-[alpha-l-rhamnopyranosyl (1-->3)] [2-O''-(E)-p-coumaroyl] [alpha-l-rhamnopyranosyl (1-->6)]-beta-d-galactoside was named as camelliquercetiside F (2), kaempferol 3-O-[alpha-l-arabinopyranosyl (1-->3)] [2-O''-(E)-p-coumaroyl] [beta-d-glucopyranosyl (1-->3)-alpha-l-rhamnopyranosyl (1-->6)]-beta-d-glucoside was named as camellikaempferoside D (3), kaempferol 3-O-[alpha-l-arabinopyranosyl (1-->3)] [2-O''-(E)-p-coumaroyl] [alpha-l-rhamnopyranosyl (1-->6)]-beta-d-glucoside was named as camellikaempferoside E (4). Chemical structures of AGFs were identified by time-of-flight mass (TOF-MS) and NMR spectrometers ((1)H NMR, (13)C NMR, (1)H-(1)H COSY, HMBC and HSQC), and the MS(2) fragmentation pathway of AGFs was further investigated. The inhibitory abilities of AGFs and their proposed metabolites on alpha-glucosidase and HMG-CoA reductase were analyzed by molecular docking simulation, and the results suggested that inhibitory activities of AGFs were significantly affected by acyl structure, number of glycosyl and conformation, and part of them had strong inhibitory activities on alpha-glucosidase and HMG-CoA reductase, suggesting that AGFs and their metabolites might be important ingredients that participate in the regulation of hypoglycemic and hypolipidemic effects. The results provided new AGFs and research directions for the practical study of FBT health functions in future.
