Effects of reaction conditions on N-epsilon-(1-Carboxymethyl)-L-lysine formation in reducing sugar-lysine mode reaction systems
作者:
Han, Wenfeng;Tan, Xinghe;Ge, Songtao
期刊:
Food Science and Technology Research ,2023年29(3):237-245 ISSN:1344-6606
通讯作者:
Han, WF
作者机构:
[Han, Wenfeng; Tan, Xinghe] Jianhu Coll, Zhejiang Ind Polytech Coll, Shaoxing 312000, Peoples R China.;[Tan, Xinghe] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.
通讯机构:
[Han, WF ] J;Jianhu Coll, Zhejiang Ind Polytech Coll, Shaoxing 312000, Peoples R China.
关键词:
N-epsilon-(1-Carboxymethyl)-L-lysine;formation rule;reducing sugar;fructose;galactose;glucose;lysine;mode reaction system
摘要:
A This study aimed to employ three reducing sugar (fructose, galactose and glucose)-lysine mode reaction systems (FLMRS, GaLMRS and GLMRS) in examining the influences of reaction conditions, such as initial pH value, reaction temperature, and time on the Nε-(1-Carboxymethyl)-L-lysine (CML) formation. Results indicated that the initial pH value were positively correlated with the CML content in the system. However, the influence of reaction temperature and time on the CML content in the system displayed an initial increasing trend followed by a decline. A temperature of 100.58/125.24/123.24 ℃ and a heating time of 30.00/24.66/25.38 min maximized the formation of CML on FLMRS/GaLMRS/GLMRS and resulted in a CML production of 2.11/1.39/1.34 mmol/mol lysine of response surface methodology. The results of this study would provide particular theoretical support for studying the formation mechanism and control methods of CML in food processing. Copyright © 2023, Japanese Society for Food Science and Technology.
语种:
英文
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油炸薯片中羧甲基赖氨酸形成规律及抑制作用的研究
作者:
韩文凤;周迎春;谭兴和
期刊:
中国粮油学报 ,2021年36(7):69-76 ISSN:1003-0174
作者机构:
[韩文凤] 浙江工业职业技术学院鉴湖学院;[周迎春] 中华人民共和国漯河海关;[谭兴和] 湖南农业大学食品科技学院
关键词:
油炸薯片;水果多酚;羧甲基赖氨酸;抑制;感官评价
摘要:
羧甲基赖氨酸(Nε - carboxymethyllysine,CML)是美拉德反应的重要产物,CML通过食品介质进入人体后,能引起多种疾病的发生。为了降低食品中CML对人体的危害,本文研究了油炸薯片中CML的形成规律和水果多酚对油炸薯片中CML含量和感官指标的影响。结果表明,油炸工艺条件对薯片中的CML含量影响显著,CML的含量与感官综合评分呈高度正相关。薯片中分别添加0.01% ~ 2.00%的葡萄籽、苹果皮或山楂皮多酚时,CML抑制率均随水果多酚添加量的增加而增大。在感官指标方面,较低添加量的水果多酚对油炸薯片的色泽几乎没有影响,较高添加量会使色泽评分显著降低;对质地、滋味与气味基本无影响。CML抑制率与油炸薯片的色泽评分高度负相关,而与质地、滋味与气味没有相关性。
语种:
中文
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Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice
作者:
Chen, Jianbing;Zhang, Chengcheng;Xia, Qile;Liu, Daqun;Tan, Xinghe* ;...
期刊:
Molecules ,2020年25(6):1302 ISSN:1420-3049
通讯作者:
Tan, Xinghe
作者机构:
[Chen, Jianbing; Tan, Xinghe] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[Liu, Daqun; Cao, Yan; Zhang, Chengcheng; Chen, Jianbing; Xia, Qile; Li, Yingdi] Zhejiang Acad Agr Sci, Minist Agr & Rural Affairs Food Sci Inst, Key Lab Post Harvest Handling Fruits, Inst Food Sci, Hangzhou 310021, Peoples R China.
通讯机构:
[Tan, Xinghe] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.
关键词:
cyclophosphamide;pectin;water;animal;chemistry;Citrus;drug effect;human;immunology;immunosuppressive treatment;Institute for Cancer Research mouse;intestine flora;mouse;Animals;Citrus;Cyclophosphamide;Gastrointestinal Microbiome;Humans;Immunosuppression;Mice;Mice, Inbred ICR;Pectins;Water
摘要:
Subcritical water can effectively hydrolyze pectin into smaller molecules while still maintaining its functional regions. Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear. Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model. In this research, immunosuppressed ICR mice were administrated with SCP at dosages of 300/600/1200 mg/kg.bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined. The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates. Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin. For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α). The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis. SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus). The results of this study suggest an innovative application of citrus pectin as an immunomodulator. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
语种:
英文
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Mechanisms of breakdown of Haematococcus pluvialis cell wall by ionic liquids, hydrochloric acid and multi-enzyme treatment
作者:
Ye, Zhang;Tan, Xing-He;Liu, Zhi-Wei* ;Aadil, Rana Muhammad;Tan, Yi-Cheng;...
期刊:
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ,2020年55(9):3182-3189 ISSN:0950-5423
通讯作者:
Liu, Zhi-Wei
作者机构:
[Tan, Yi-Cheng; Tan, Xing-He; Liu, Zhi-Wei; Ye, Zhang] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.;[Inam-ur-Raheem, Muhammad; Aadil, Rana Muhammad] Univ Agr Faisalabad, Natl Inst Food Sci & Technol, Faisalabad 38000, Pakistan.
通讯机构:
[Liu, Zhi-Wei] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.
关键词:
Astaxanthin extractability;disruption mechanism;FTIR;Haematococcus pluvialis;structural analysis;X-ray diffraction
摘要:
<jats:title>Summary</jats:title><jats:p>The robust cell wall structure of <jats:italic>Haematococcus pluvialis</jats:italic> (<jats:italic>H.pluvialis</jats:italic>) consists of polysaccharides and tough non‐hydrolysable sporopollenins, which makes it difficult to extract superpotent antioxidant, astaxanthin from these cells. Therefore, breakdown of cell wall is a key step in the overall process of astaxanthin recovery. In this study, the mechanism of three well‐established chemical techniques for cell disruption of <jats:italic>H.pluvialis</jats:italic> cysts [ionic liquids (IL), hydrochloric acid (HCl) and multiple enzymes (multi‐enzyme, ME)] on deconstruction of the cyst cell wall of <jats:italic>H.pluvialis</jats:italic> was explored and characterised by Fourier transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC‐MS) analyses. The results demonstrated that the three cell wall breakdown techniques exhibited high extraction efficacies for the recovery of astaxanthin from <jats:italic>H.pluvialis</jats:italic> [IL (86.71±2.06%), HCl (80.52±2.28%) and ME (71.08±2.49%)]. However, their performances on disrupting the trilayered cell walls of <jats:italic>H.pluvialis</jats:italic> were significantly different, which were confirmed by distinct morphologies of the treated cell walls visualised by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Meanwhile, the results of FTIR confirmed that, to some extent, cellulose, hemicellulose and lignin in the cell walls were hydrolysed by HCl, IL and ME treatments. However, ME exhibited a less hydrolytic effect on lignin than HCl and IL. Moreover, XRD and NMR analyses implied that the amorphous region of cell wall was susceptible to hydrolysis/breakdown by the three techniques.</jats:p>
语种:
英文
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基于焙烤食品的赖氨酸-葡萄糖模式反应体系中羧甲基赖氨酸的形成规律
作者:
韩文凤;华向美;谭兴和
期刊:
中国粮油学报 ,2019年34(12):41-46,58 ISSN:1003-0174
作者机构:
湖南农业大学食品科技学院;漯河职业技术学院食品工程系;[华向美] 漯河出入境检验检疫局;[谭兴和] 食品科学与生物技术湖南省重点实验室;[韩文凤] 湖南农业大学
关键词:
羧甲基赖氨酸;形成规律;赖氨酸;葡萄糖;模式体系;焙烤食品
摘要:
通过建立基于焙烤食品的赖氨酸-葡萄糖模式反应体系并利用超高效液相色谱-串联质谱UPLC-MS/MS检测方法,研究pH、底物浓度,反应温度与反应时间等工艺条件对羧甲基赖氨酸形成的影响及羧甲基赖氨酸含量的动力学变化规律。结果表明,pH值、底物浓度和反应时间与体系中羧甲基赖氨酸含量均呈正相关;而反应温度对体系中羧甲基赖氨酸含量的影响较为复杂,呈先增大后减小的趋势,在pH值为7.0,底物浓度为0.3 mol/L和反应时间为30 min的条件下,反应温度为100℃时羧甲基赖氨酸的含量达到最大值为317.37 mg/mol赖氨酸。动力学研究发现在低温加热的反应体系中,羧甲基赖氨酸的含量随着反应时间的延长而增大;而在高温加热的反应体系中,羧甲基赖氨酸的含量随着反应时间的延长呈先增大后减小的趋势。本实验为研究谷物类焙烤食品加工过程中羧甲基赖氨酸的形成和抑制机理提供一定的参考。
语种:
中文
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微波-热风联用制取笋干工艺条件优化
作者:
姚荷;谭亦成;谭兴和;李清明;王锋;...
期刊:
食品科学 ,2019年40(12):260-266 ISSN:1002-6630
作者机构:
湖南农业大学食品科学技术学院;[王锋; 张春艳; 谭亦成; 姚荷; 李清明; 谭兴和] 食品科学与生物技术湖南省重点实验室;[成才良; 王栏树] 湖南佳宴食品有限公司;[秦长光] 龙山县昶光农业科技发展有限公司
关键词:
竹笋;响应面法;微波;热风;干燥
摘要:
为获得干燥时间短、干燥品质好的笋干,以竹笋为原料,在单因素试验的基础上,根据中心组合试验设计原理,分析微波干燥功率、微波干燥时间和热风干燥温度3 个因素对笋干感官评分、总干燥时间、复水比、色差和硬度指标的影响,以确定微波-热风联用制取笋干最佳工艺条件。结果表明,制取笋干的最佳工艺条件为:微波干燥功率6.3 W/g、微波干燥时间60 s、热风干燥温度65 ℃。在此条件下得到的笋干感官评分85.6、总干燥时间200 min、复水比6.17,干制品色差ΔE* 19.99、复水制品色差ΔE* 13.92、干制品硬度19 511.23 g、复水制品硬度20 010.71 g,该工艺研究结果可为笋干产业化发展提供理论支持。
语种:
中文
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响应面法优化复合酶提取雨生红球藻中虾青素的工艺
作者:
张晔;刘志伟;谭兴和
期刊:
食品工业科技 ,2019年40(22):87-92 ISSN:1002-0306
作者机构:
湖南农业大学食品科技学院,食品科学与生物技术湖南省重点实验室,湖南长沙410000;[张晔; 刘志伟; 谭兴和] 湖南农业大学
关键词:
雨生红球藻;虾青素;复合酶法;提取工艺;工艺优化
摘要:
本文研究了纤维素酶、果胶酶以及复合酶对雨生红球藻破壁效果,并通过响应面优化酶解破壁辅助提取虾青素的工艺.结果 表明,当纤维素酶和果胶酶酶活力配比1∶1 (U/U),加酶量7000 U/mL时,优化的最佳酶解条件为:酶解pH4.9,酶解温度49℃,酶解时间为6h,理论提取率为70.21%,实际提取率为71.08%±0.26%,相对误差为1.2%.综上,复合酶法辅助提取方法简单、条件温和、绿色安全、效率高,可在雨生红球藻虾青素实际提取工艺中得到应用.
语种:
中文
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麦胚蛋白双酶酶解物的制备及其体外醒酒活性的研究
作者:
罗思媛;郭红英;张琳娜;王锋;谭兴和;...
期刊:
中国粮油学报 ,2018年33(5):87-93 ISSN:1003-0174
通讯作者:
Guo, H.
作者机构:
湖南农业大学食品科学与技术学院, 长沙, 410128;食品科学与生物技术湖南省重点实验室, 食品科学与生物技术湖南省重点实验室, 长沙, 410128;[罗思媛; 张琳娜; 何蕾] 湖南农业大学食品科学与技术学院, 长沙, 410128;[郭红英; 王锋; 谭兴和] 湖南农业大学食品科学与技术学院, 长沙, 410128 食品科学与生物技术湖南省重点实验室, 食品科学与生物技术湖南省重点实验室, 长沙, 410128
通讯机构:
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词:
麦胚蛋白;双酶酶解;肽得率;乙醇脱氢酶;醒酒活性
摘要:
为充分利用麦胚资源,制备具有醒酒作用的麦胚肽,本研究以Na2C03预处理过的麦胚为原料,以肽得率(TCA-PSI)、水解度(DH)及乙醇脱氢酶(ADH)激活率为指标,研究了碱性蛋白酶(Alcalase)、中性蛋白酶(Neutral)双酶分步酶解工艺及酶解物的醒酒活性.结果表明,双酶分步酶解的最优工艺条件为麦胚蛋白质量分数为3.5% ,Alcalase添加量4 000 U/g,Neutral添加量1 000 U/g,所得酶解物的TCA -PSI、DH、ADH激活率分别为:75.49%、65.18%、68.37%.表明麦胚蛋白双酶酶解物可以显著提高ADH的活力,具有较好的体外醒酒活性.
语种:
中文
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桂淮系列淮山淀粉结构研究
作者:
赵小梅;李清明;苏小军;郭时印;熊兴耀;...
期刊:
中国粮油学报 ,2018年33(7):58-64 ISSN:1003-0174
通讯作者:
Li, Q.
作者机构:
[赵小梅] 湖南农业大学食品科学与技术学院,长沙410128;[赵小梅] 永州工贸学校,永州425000;湖南农业大学食品科学与技术学院,长沙,410128;中国农业科学院蔬菜花卉研究所,北京,100081;广西农业科学院经济作物研究所,南宁,530007
通讯机构:
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词:
淮山;淀粉;红外光谱;核磁共振;结构特性
摘要:
选育产量高、淀粉含量高、适应性广的淮山新品种对保障我国粮食安全和促进农民增收具有重大意义,而淮山淀粉的结构与性质可以影响淮山的口感以及淀粉加工特性。通过测定桂淮5号、桂淮7号、桂淮8号、紫淮山4个淮山品种的淀粉的成分、淀粉颗粒的粒径与外观,利用偏光十字、红外光谱、核磁共振等分析淀粉颗粒的结晶特性,研究不同品种间淀粉结构与性质。结果表明:桂淮8号较其余3个品种淮山淀粉具有更高的蛋白质和直链淀粉含量;X-射线衍射及~(13)C谱结果分析显示桂淮7号和桂淮8号为B型淀粉。
语种:
中文
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植物乳杆菌发酵萝卜干品质变化分析
作者:
刘宗敏;谭兴和;周红丽;王锋;郭红英;...
期刊:
食品科学 ,2018年39(2):198-202 ISSN:1002-6630
通讯作者:
Tan, Xinghe(xinghetan@163.com)
作者机构:
[郭红英; 姚荷; 刘楚岑; 王锋; 周红丽; 谭兴和; 刘宗敏] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[严钦武; 徐永兵] Hunan Chaqi Vegetables Industry Co. Ltd., Yueyang, 414000, China;[王栏树] Hunan Jiayan Food Co. Ltd., Changsha, 410000, China
关键词:
植物乳杆菌;发酵;萝卜干;亚硝酸盐;品质
摘要:
为加快发酵速率,降低产品亚硝酸盐含量,并提高产品品质,本研究以萝卜干为原料,分别接种植物乳杆菌L4(Lactobacillus plantarum L4)和植物乳杆菌B5(L. plantarum B5),并以自然发酵为对照,萝卜干发酵时间为56 d,研究L4和B5对萝卜干品质的影响。结果表明:L4、B5和自然发酵pH值降低的速率依次为: L4>B5>自然发酵。亚硝酸盐含量随着发酵时间的延长先增加后减小,其中L4发酵在22 d左右出现亚硝酸盐峰,峰值为(3.23±0.17)mg/kg,B5和自然发酵在33 d左右出现亚硝酸盐峰,峰值分别为(2.04±0.12)mg/kg和(3.79±0.25)mg/kg(P<0.05)。挥发酯含量、游离氨基酸含量都随着发酵时间的延长呈上升趋势。L*、b*随着发酵时间的延长呈下降趋势,而a*随着发酵时间的延长呈上升趋势。发酵结束时,L4、B5和自然发酵感官评分别为88.7±2.56、81.8±1.49和74.1±3.88。由此表明,植物乳杆菌L4和B5可以缩短萝卜干的发酵周期,提高萝卜干安全性和品质,其中L4表现比B5好。
语种:
中文
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食品中羧甲基赖氨酸的形成机理和抑制途径研究进展
作者:
韩文凤;谭兴和;林晓丽;邱泼;孟宏昌;...
期刊:
现代食品科技 ,2018年34(1):245-251 ISSN:1673-9078
通讯作者:
Tan, X.-H.
作者机构:
湖南农业大学食品科技学院;漯河职业技术学院食品工程系;[谭兴和] 食品科学与生物技术湖南省重点实验室;[邱泼] 浙江麦吉士食品有限公司;[林晓丽; 孟宏昌; 贾娟] 漯河职业技术学院
通讯机构:
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词:
羧甲基赖氨酸;危害;形成机理;抑制途径
摘要:
本文研究建立一种检测新型瘦肉精多残留胶体金检测卡的方法,能对大量样品进行定量检测。应用胶体金免疫层析技术,采用柠檬酸钠还原法,标记新型瘦肉精多克隆抗体并喷于玻璃纤维上,新型瘦肉精偶联OVA抗原和羊抗鼠Ig G分别结合于硝酸纤维膜上,依次将样品垫、胶体金垫、硝酸纤维素膜和吸水纸组装切割成新型瘦肉精多残留胶体金检测卡。通过灵敏度、假阳性、假阴性试验测试,梯度浓度显色结果表明,该检测卡的灵敏度分别为克伦特罗3 ng/m L、莱克多巴胺5 ng/m L、沙丁胺醇3 ng/m L、西马特罗5 ng/m L、班布特罗5 ng/m L、妥布特罗5 ng/m L、特布他林8 ng/m L、氯丙那林5 ng/m L、马布特罗5 ng/m L、溴布特罗8 ng/m L,检测时间为3 min,检测滴加的最优尿液量为7090μL,批内和批间重复性为100%,假阳性率和假阴性率均为0。联检技术能实现现场快速检测,提高检测速度和准确性、降低检测成本,可作为生产过程监管和大批量样本的筛选。
语种:
中文
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黑花生衣提取物抑制油炸马铃薯体系中丙烯酰胺形成的研究
作者:
欧阳燕林;王锋;谭兴和;郭红英;张春艳;...
期刊:
现代食品科技 ,2017年33(2):123-128 and 93 ISSN:1673-9078
通讯作者:
Tan, X.-H.
作者机构:
湖南农业大学食品科学与技术学院,食品科学与生物技术湖南省重点实验室,湖南长沙410128;[王锋; 张春艳; 张喻; 郭红英; 谭兴和; 欧阳燕林] 湖南农业大学
通讯机构:
Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词:
黑花生衣;花色苷;原花青素;丙烯酰胺
摘要:
本论文研究黑花生衣提取物对油炸马铃薯体系中丙烯酰胺形成的影响。采用黑花生衣中提取出的粗提物(BPSE)、原花青素(BPSP)、花色苷(BPSA)以及BPSP分级得到的M1、M2和M3三种不同平均聚合度的原花青素,分别将6种物质添加到马铃薯粉中,油炸后,用高效液相色谱检测丙烯酰胺含量;选择最佳抑制率最大的物质进行动力学试验。结果表明,BPSE、BPSP、BPSA、M1、M2和M3对丙烯酰胺的最佳抑制浓度分别为0.1μg/mL、1μg/mL、0.1μg/mL、1μg/mL、0.01μg/mL和0.1μg/mL,最佳抑制率分别为39.78%、60.63%、25.18%、53.35%、49.02%和43.75%,原花青素能很好地抑制丙烯酰胺形成。通过比较BPSP试验组、空白对照组和橙皮苷阳性对照组对油炸马铃薯体系中丙烯酰胺的形成动力学过程,得出丙烯酰胺在200 s时,生成量最高,BPSP可以有效抑制丙烯酰胺的形成过程。
语种:
中文
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油菜秸秆~(60)Co–γ辐照降解产物分析
作者:
张春艳;谭兴和;熊兴耀;苏小军;李清明;...
期刊:
湖南农业大学学报(自然科学版) ,2017年43(1):92-97 ISSN:1007-1032
作者机构:
湖南农业大学食品科学技术学院, 湖南, 长沙, 410128;中国农业科学院蔬菜与花卉研究所, 湖南省作物种质创新与资源利用重点实验室, 北京, 100081;湖南农业大学食品科学技术学院, 湖南省作物种质创新与资源利用重点实验室, 湖南, 长沙, 410128;湖南省农业科学院核农学与航天育种研究院, 湖南, 长沙, 410125;[张春艳; 谭兴和; 李清明] 湖南农业大学食品科学技术学院, 湖南, 长沙, 410128
关键词:
油菜秸秆;~(60)Co–γ辐照;降解产物
摘要:
以油菜秸秆为材料,采用~(60)Co–γ辐照,设6个辐照剂量处理,分别为0(CK)、400、600、800、1 000、 1 200 kGy,考察不同剂量辐照处理后油菜秸秆3大主要组分降解率的变化,并采用气质联用法对辐照后全产物进行分析。结果表明:随着剂量的增大,纤维素、半纤维素和木质素降解率都有不同程度的提高;当辐照剂量为1 200 kGy时,纤维素、半纤维素和木质素降解率分别达到69.06%、42.57%和4.99%;未辐照油菜秸秆水提液乙酸乙酯萃取物中分别检测到脂肪酸类物质22种、芳香类物质5种,未检测到呋喃类物质,而从不同剂量辐照后样品中分别检测到脂肪酸类物质25种、芳香类物质17种,呋喃类物质5种,且各类产物的种类与含量变化呈现出不同规律。
语种:
中文
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食品中羧甲基赖氨酸的危害和检测方法的研究进展
作者:
韩文凤;谭兴和;林晓丽;邱泼;孟宏昌
期刊:
现代食品科技 ,2017年33(9):299-305 ISSN:1673-9078
通讯作者:
Tan, X.-H.
作者机构:
湖南农业大学食品科技学院;漯河职业技术学院食品工程系;[谭兴和] 食品科学与生物技术湖南省重点实验室;[邱泼] 浙江麦吉士食品有限公司;[林晓丽; 孟宏昌] 漯河职业技术学院
通讯机构:
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词:
羧甲基赖氨酸;危害;酶标法;气相色谱法;液相色谱法;质谱法
摘要:
食品中的N^ε-(1-羧甲基)-L-赖氨酸(N^ε-(1-Carboxyethyl)-L-Lysine,简称羧甲基赖氨酸或CML)是还原糖的羰基与蛋白质、氨基酸的氨基等通过美拉德反应产生的晚期糖基化终末产物中的一种主要成分。CML具有较高的酸稳定性,可以作为评价食品体系美拉德反应中蛋白质化学修饰、羰基应激和脂质氧化的一个重要指标。CML通过食品介质进入人体后,可积聚于多个不同的组织器官中。达到一定数量后,会直接影响组织器官的功能,导致机体的病理变化。CML与人体许多疾病的发生密切相关,能促进糖尿病、肾病、动脉粥样硬化等疾病的发展和人体器官的快速衰老。CML的定量检测对指导人们减少CML的摄入,进而降低相关疾病的患病风险有重要的意义。本文就CML的危害和检测方法进行综述,以期为寻找具有高灵敏度、高选择性和快速高效的CML含量的检测方法提供依据。
语种:
中文
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Bacterial Diversity during Natural Fermentation of Soybean Paste under Variable Temperature Conditions
作者:
周红丽;李莎;张灵;蒋立文;谭兴和
期刊:
食品科学 ,2017年38(14):120-126 ISSN:1002-6630
通讯作者:
Tan, Xinghe(xinghetan@163.com)
作者机构:
[李莎; 蒋立文; 张灵; 谭兴和; 周红丽] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[蒋立文; 谭兴和; 周红丽] Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha, 410128, China
关键词:
温度模式;豆瓣酱;细菌;多样性
摘要:
通过DNA提取,聚合酶链式反应扩增以及高通量测序技术对不同温度发酵模式下的豆瓣酱中的细菌进行多样性分析。结果表明:先低温后高温发酵模式下样品得到了2 081个操作分类单元(operational taxonomic unit, OTU),而在先高温后低温模式中为1 870个OTU。在门类水平上,2种发酵模式中的相对丰度值最高的均为厚壁菌门,分别为72.24%(先低温后高温)和43.84%(先高温后低温);属类水平上为葡萄球菌属,其相对丰度值分别为44.13%和32.52%。先低温后高温发酵模式下细菌物种的丰富度和多样性普遍高于先高温后低温的发酵模式。
语种:
中文
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不同乳酸菌发酵萝卜干挥发性成分分析
作者:
刘宗敏;谭兴和;周红丽;李清明;王锋;...
期刊:
食品科学 ,2017年38(24):144-149 ISSN:1002-6630
通讯作者:
Tan, Xinghe(xinghetan@163.com)
作者机构:
[郭红英; 姚荷; 李清明; 刘楚岑; 王锋; 周红丽; 谭兴和; 刘宗敏] College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[严钦武; 徐永兵] Hunan Cha Qi Vegetables Industry Co. Ltd., Yueyang, 414000, China;[王栏树] Hunan Jiayan Food Co. Ltd., Changsha, 410000, China
关键词:
乳酸菌;发酵;萝卜干;气相色谱-质谱联用;挥发性成分
摘要:
为了解不同乳酸菌对发酵萝卜干挥发性成分的影响,用顶空固相微萃取法预处理发酵萝卜干样品,结合气相色谱-质谱联用仪,分析肠膜明串珠菌(B1)、玉米乳杆菌(B2)、副干酪乳杆菌(B3)、乳酸乳球菌(B4)、植物乳杆菌(B5)、植物乳杆菌(L4)和自然发酵(对照)萝卜干中挥发性成分。7组发酵萝卜干中共检测出8类77种挥发性成分,其中含有6种相同成分:苯乙醇、辛酸乙酯、癸酸乙酯、壬酸乙酯、庚酸乙酯、己酸异戊酯。不同菌种发酵萝卜干挥发性成分种类及相对含量有较大差异,B1检出挥发性成分6类35种,B2检出6类25种,B3检出5类19种,B4检出7类30种,B5检出7类33种、L4检出6类31种,自然发酵检出7类37种。发酵萝卜干中的挥发性成分主要包括醇类、酸类、酯类、醛酮类和烯烃类等。自然发酵萝卜干挥发性成分种类多,但大多成分相对含量较低;B2和B3发酵萝卜干挥发性成分种类较少且相对含量较低;B1、B4、B5和L4能促进发酵萝卜干特有风味物质的形成,其中B1能促进醇、烯烃的形成,B4能促进醇、酯和烯烃的形成,L4能促进酯、酮醛和烯烃的形成,B5能促进醇、酯、酮醛和烯烃的形成。
语种:
中文
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多种方式加工黄花菜
作者:
谭兴和
期刊:
湖南农业 ,2016年(11):7 ISSN:1005-362X
作者机构:
湖南农业大学食品科技学院 410125;[谭兴和] 湖南农业大学
关键词:
黄花菜;加工;特色产业;农民增收;祁东县;消费习惯;持续发展;产量高
摘要:
<正>祁东县黄花菜面积大,产量高,种植农户多,黄花菜已经成为祁东县的特色产业,种植、加工黄花菜,已经成为祁东县农民增加收入的重要途径。全国各地都有黄花菜的消费习惯,搞好黄花菜保鲜与加工,确保黄花菜产业健康持续发展,对于祁东县农民增收致富意义重大。1.无硫干黄花菜干黄花菜是一种传统加工食品,干制以后,能长期保存,周年销售、食用。但是,采用亚硫酸盐护色——分散干制传统方法制成的干黄花菜,在贮藏期间颜色逐渐发黑,尽管不影响食用价值,但严重影响到外观品质和市场销售。现代加工技术证明,推广使
语种:
中文
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Analysis of degradation products and structural characterization of giant reed and Chinese silvergrass pretreated by 60Co-γ irradiation
作者:
Li, Qing-ming;Li, Xia-jie;Jiang, Yi-le;Xiong, Xing-yao;Hu, Qiu-long;...
期刊:
Industrial Crops and Products ,2016年83:307-315 ISSN:0926-6690
通讯作者:
Su, Xiao-jun
作者机构:
[Li, Qing-ming; Li, Xia-jie; Tan, Xing-he; Jiang, Yi-le] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Li, Qing-ming; Wang, Ke-qin; Su, Xiao-jun; Hu, Qiu-long] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Su, Xiao-jun] Hunan Agr Univ, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Xiong, Xing-yao] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.
通讯机构:
[Su, Xiao-jun] H;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.
关键词:
Ethanol;Gamma irradiation;Inhibitor;Pretreatment
摘要:
Radiation pretreatment of lignocellulosics has been widely applied for the breakdown of the stubborn cellulose structure to enable the cellulose to be more accessible to cellulolytic enzymes. However, the effects of gamma irradiation on the lignocellulosics structure changes and the composition of pretreatment degradation products are not well understood. In this study, the influences of irradiation dose on the microstructure and irradiated-degradation components of cellulose under Co-6 gamma-irradiation (0-1200 kGy) was comprehensively investigated using giant reed and Chinese silvergrass as model substrate. The microstructure of biomass was examined with Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Additionally, composition determination and degradation products were analyzed. FT-IR analysis show that irradiation destroyed the glycosidic bond and inter- and intramolecular hydrogen bond of cellulose. XRD and SEM analyses confirm that irradiation could damage the crystalline microstructure and surface morphology of lignocellulosics. Ion chromatography analysis demonstrates that there exist fermentation sugars such as glucose, xylose, arabinose, galactose and cellobiose. Moreover, some potential inhibitors, such as glucuronic acid, galacturonic acid, methanoic acid, acetic acid, furfuraldehyde and p-coumaric acid, were detected in the irradiation-derived degradation products. Irradiation dose exhibited obvious influences on the production of these products. These investigations would be helpful for further understanding the applications and fundamentals of gamma irradiation pretreatment for bioethanol production from lignocellulosics. (c) 2016 Elsevier B.V. All rights reserved.
语种:
英文
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60Co-γ radiation-induced changes in the physical and chemical properties of rapeseed straw
作者:
Zhang, ChunYan;Su, XiaoJun;Xiong, XingYao;Hu, QiuLong;Amartey, Samuel;...
期刊:
Biomass & Bioenergy ,2016年85:207-214 ISSN:0961-9534
通讯作者:
Tan, XingHe
作者机构:
[Zhang, ChunYan; Su, XiaoJun; Tan, XingHe] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Su, XiaoJun; Xiong, XingYao; Hu, QiuLong] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Su, XiaoJun] Hunan Agr Univ, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Changsha 410128, Hunan, Peoples R China.;[Xiong, XingYao] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.;[Amartey, Samuel] Univ London Imperial Coll Sci Technol & Med, Div Biol, London E8 1PQ, England.
通讯机构:
[Tan, XingHe] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词:
Brassica napus L.;gamma-ray irradiation;Microstructure;Reactivity
摘要:
We investigated changes in the physical and chemical properties of rapeseed straw after treatment with different doses of 60Co γ-irradiation (0 kGy-1200 kGy). Raman spectra, electron spin resonance (ESR), and nuclear magnetic resonance (NMR) analyses of the pretreated samples showed that the irradiation partially destroyed the intra- or intermolecular structure of rapeseed straw. Particle size distribution and specific surface area analyses suggested that irradiation decreased the particle size, narrowed the distribution range, and increased the specific surface area. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) curves showed that increasing the irradiated dose decreased the thermal stability of the treated rapeseed straw and increased the reactivity. Elemental analyses suggested that the oxygen content slightly increased, suggesting that oxygen in the air may be involved in the reaction. These results demonstrate that γ-irradiation can induce a series of changes in the physical and chemical properties of rapeseed straw. © 2015 Elsevier Ltd.
语种:
英文
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EFFECT OF (CO)-C-60-gamma IRRADIATION ON THE MICROSTRUCTURE AND ENZYMATIC HYDROLYSIS OF RAPESEED STRAW
作者:
Zhang, C. Y.;Su, X. J.;Hu, Q. L.;Zhang, T.;Tan, X. H.* ;...
期刊:
Cellulose Chemistry and Technology ,2016年50(9-10):973-981 ISSN:0576-9787
通讯作者:
Tan, X. H.;Xiong, X. Y.
作者机构:
[Tan, X. H.; Su, X. J.; Zhang, C. Y.] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Hu, Q. L.; Xiong, X. Y.; Su, X. J.] Hunan Agr Univ, Key Lab Corp Germplasm Innovat & Resource Utiliza, Changsha 410128, Hunan, Peoples R China.;[Zhang, T.] Hunan Biol & Electromech Polytech, Changsha 410127, Hunan, Peoples R China.;[Xiong, X. Y.] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.
通讯机构:
[Tan, X. H.; Xiong, X. Y.] H;[Xiong, X. Y.] C;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Key Lab Corp Germplasm Innovat & Resource Utiliza, Changsha 410128, Hunan, Peoples R China.;Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.
关键词:
rapeseed straw;gamma-ray irradiation;enzymatic hydrolysis;microstructure;total reducing sugar;sugar compounds
摘要:
In this study, rapeseed straw was pretreated with Co-60-gamma radiation and digested with cellulase to improve sugar production. The compositions of straw were analyzed. Results showed that there were remarkable changes in total reducing sugar and an increase in the degradation of cellulose, hemicellulose and lignin. At a dose of 1200 kGy, 39.3 times more reducing sugar from the irradiated straw was released, as compared to the control (not irradiated) straw (35.34 vs. 0.90 mg/g). Furthermore, both the total content and the number of species of sugar compounds increased. Enzymatic digestion of the irradiated straw resulted in the degradation of 79.21% and 75.59% of cellulose and hemicellulose, respectively. The total reducing sugar increased by a factor of 4.6 to 392.50 mg/g in the irradiated straw from 86.18 mg/g in the control, with greater total content and more species of sugar compounds. Analyses with X-ray diffraction, infrared spectroscopy, and scanning electron microscopy showed that the microstructure of the irradiated straw was destroyed.
语种:
英文
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