In this work, we engineered the alpha/beta fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the alpha/beta fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high activity and stability at higher temperature and broader pH. This quartet variant was characterized by an almost threefold increased activity and a sevenfold increased stability compared to native mannanase Man23. Our results suggest that such work is safe to increase our target protein stability with no loss of activity.
English teachers;paternalistic leadership;emotional creativity;professional identity;teaching efficacy
Using social exchange theory and the social constructivist theory of emotion, I examined the relationship between supervisors' paternalistic leadership and college English teachers' teaching efficacy in China, as well as the roles emotional creativity and professional identity played in this relationship. Participants were 674 teachers of English at 30 colleges in China. Results of factor and correlation analyses, structural equation modeling, and regression analysis revealed that supervisors' paternalistic leadership had significantly positive effects on teachers' teaching efficacy, and that teachers' professional identity had a meditating effect in the relationship between paternalistic leadership and teaching efficacy. In addition, teachers' emotional creativity positively moderated the relationship between supervisors' paternalistic leadership and teachers' teaching efficacy, and emotional creativity acted as a mediated moderator of the link between these two variables. My findings contribute to comprehension of the effect mechanism of supervisors' paternalistic leadership on teachers' teaching efficacy.
Advance Journal of Food Science and Technology,2013年5(2):99-105 ISSN：2042-4868
[Fan, Zhi-Yong; Zhou, Ping] Department of Foreign Language, Hunan Agricultural University, Changsha, 410128, China;[Fan, Zhi-Yong; Liu, Guo-Hua] Key Laboratory of Feed Biotechnology, Ministry of Agriculture of the People's Republic of China, Beijing, 100081, China;[Fan, Zhi-Yong; Liu, Zhong-Hua] Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, 410128, China;[Fan, Zhi-Yong; Wu, Xiao-Song] Engineering Research Center for Feed Safety and Efficient Utilization, Ministry of Education, Hunan Agricultural University, Changsha, 410128, China;[Liang, Zhe; Zhou, Ding-Gang] Department of Animal Science and Technology, Sicau Agricultural University, Yaan, 625014, China
Applied Biochemistry and Biotechnology,2011年163(1):186-194 ISSN：0273-2289
[Zhou, Hai-Yan] Center of Analysis and Measurement, Hunan Agricultural University, Changsha 410128, China;[Rao, Li-Qun; Wu, Yong-Yao] College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;[Pan, Hong-Ya] College of Foreign Language, Hunan Agricultural University, Changsha 410128, China
[Zhou Lijuan; Yue Xiaobo] College of Computer and Communication Engineering, Changsha University of Science and Technology, Changsha 410114, China;[Zhou Lijuan] College of Information Science and Technology, Hunan Agricultural University, Changsha 410128, China;[Zhou Lijun] College of Foreign Languages, Hunan Agricultural University, Changsha 410128, China
3rd International Conference on Digital Image Processing (ICDIP 2011)