Swelling of Serum-Stable DNA Nanoparticles upon Target-Induced Conformational Rearrangement of Sensing Probes for the Signal-On Detection of Cancer-Related Genes
作者：
Xue, Chang;Huang, Hong;Wang, Lei;Liao, Wenqiang;Jiang, Hao;...
期刊：
Analytical Chemistry ,2022年94(6):2749-2756 ISSN：0003-2700
通讯作者：
Wu, ZS
作者机构：
[Xue, Chang; Wu, Zai-Sheng; Liao, Wenqiang; Wang, Lei; Jiang, Hao; Huang, Hong] Fuzhou Univ, Coll Chem,State Key Lab Photocatalysis Energy & E, Canc Metastasis Alert & Prevent Ctr,Pharmaceut Ph, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350108, Peoples R China.;[Wang, Lei] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.
通讯机构：
[Wu, ZS ] F;Fuzhou Univ, Coll Chem,State Key Lab Photocatalysis Energy & E, Canc Metastasis Alert & Prevent Ctr,Pharmaceut Ph, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350108, Peoples R China.
摘要：
Nuclease-resistant assay probes are of significant importance for biochemical analysis and disease diagnosis. In this contribution, a reconfigurable lipidic moiety-attached DNA nanoparticle (LDN) is constructed from a cholesterol-conjugated multifunctional hairpin-type DNA probe (Chol-DP) by hydrophobicity-mediated self-assembly. The LDN holds high serum stability and displays a low false-positive signal even in a complex biological milieu. The hydrophobic cholesterol moiety enables the hydrophobicity-mediated assembly, while hydrophilic DNA sequence serves as a recognition element and a polymerization template. The initiator-activated strand displacement amplification (SDA) reaction can convert the hairpin-shaped probe into rigid double-stranded DNA (dsDNA), causing the conformational rearrangement-based LDN swelling that can be used to reliably and fluorescently signal the cancer-related p53 gene. The size increase and structural reconfiguration are confirmed by dynamic light scattering (DLS) analysis and confocal microscopy imaging, respectively. Target p53 is specifically detected down to 10 pM. The whole assay process involved only several simple mixing steps. Recovery test and blind test further confirm the feasibility of the use of the LDN for the detection of target DNA in a complex biological milieu, indicating a promising nanotool for biomedical applications.
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英文
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Accessing deep-red emission using chemical units cosubstituted LaTiSbO6:Mn4+ phosphor
作者：
Zhang, Xiaojuan;Ma, Yibiao;Gao, Peixin;Su, Liujuan;Zhang, Zengtao;...
期刊：
Ceramics International ,2022年48(20):29547-29553 ISSN：0272-8842
通讯作者：
Lu, Xiangyang(xiangyangcn@163.com);Xia, Mao(xiamao2014@163.com)
作者机构：
[Zhang, Xiaojuan; Lu, Xiangyang] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Xia, Mao; Su, Liujuan; Ma, Yibiao; Zhang, Zengtao; Zhou, Zhi; Gao, Peixin] Hunan Agr Univ, Sch Chem & Mat Sci, Changsha 410128, Peoples R China.;[Xia, Mao; Su, Liujuan; Zhang, Xiaojuan; Lu, Xiangyang; Ma, Yibiao; Zhang, Zengtao; Zhou, Zhi; Gao, Peixin] Hunan Opt Agr Engn Technol Res Ctr, Changsha 410128, Peoples R China.
通讯机构：
[Xiangyang Lu] C;[Mao Xia] S;School of Chemistry and Materials Science, Hunan Agricultural University, Changsha, 410128, PR China<&wdkj&>Hunan Optical Agriculture Engineering Technology Research Center, Changsha, 410128, PR China<&wdkj&>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, PR China<&wdkj&>Hunan Optical Agriculture Engineering Technology Research Center, Changsha, 410128, PR China
关键词：
Chemical unit cosubstitution;LaTiSbO6:Mn4+;Mn4+ ions;Plant growth LED lighting
摘要：
Red light-emitting phosphors are important to the field of plant lighting. Therefore, it is necessary to further optimize phosphor materials. Herein, we synthesized a sequence of phosphor LaTiSbO6:Mn4+ (LTS:Mn4+). Due to the 2E to 4A2 transition, LTS:Mn4+ phosphors can emit red light in the range of 620–780 nm, with an emission peak at 687 nm. Chemical unit cosubstitution (substituting W6+ - Al3+/W6+ - Ga3+ for Ti4+ - Sb5+) was used as a method to enhance the luminescence properties of LTS:Mn4+. When the substitution ratio of W6+ - Ga3+ and W6+ - Al3+ reached 0.1% and 0.75%, respectively, the luminescence intensity increased to 204% and 182%. Using the LTS:Mn4+, W6+, Ga3+ phosphor and a 470 nm blue chip to fabricate a pc-LED device, the electroluminescence (EL) spectrum is well matched with the phytochrome absorption range. Therefore, the LTS:Mn4+ phosphor will be very promising for plant growth. © 2022
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英文
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Tetrahedron-Based Constitutional Dynamic Network for COVID-19 or Other Coronaviruses Diagnostics and Its Logic Gate Applications
作者：
Pan, Jiafeng;He, Ying;Liu, Zhi;Chen, Junhua*
期刊：
Analytical Chemistry ,2022年94(2):714-722 ISSN：0003-2700
通讯作者：
Chen, Junhua
作者机构：
[Chen, Junhua; Pan, Jiafeng] Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Integrated Agroenvironm Pollut, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangzhou 510650, Peoples R China.;[Liu, Zhi; He, Ying] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Chen, JH ] G;Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Integrated Agroenvironm Pollut, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangzhou 510650, Peoples R China.
摘要：
Considering the large-scale outbreak of the coronavirus, it is essential to develop a versatile sensing system for different coronaviruses diagnostics, such as COVID-19, severe acute respiratory syndrome-related coronavirus (SARS-CoV), and bat SARS-like coronavirus (Bat-SL-CoVZC45). In this work, a tetrahedron-based constitutional dynamic network was built as the sensing platform for coronavirus detection. Four different DNA probes were used to construct the tetrahedron structure. DNAzyme and the fluorophore modified substrate strand were used to generate different fluorescence signals, which can be used to distinguish different coronaviruses. The coronavirus biosensor shows a high sensitivity for COVID-19, Bat-SL-CoVZC45, and SARS-CoV detection, with detection limits of 2.5, 3.1, and 2.9 fM, respectively. Also, the platform is robust, and the possible interference from clinical samples was negligible. Using different coronaviruses as inputs, we have fabricated several concatenated logic gates, such as “AND–OR”, “INHIBIT–AND”, “AND–AND–AND”, and “AND–INHIBIT”. Importantly, our logic system can also be used to identify SARS-CoV-2 Delta and Lambda variants in the logic operations. Due to the unique advantages of high sensitivity and selectivity, multiple logic biocomputing capabilities, and multireadout mode, this flexible sensing system provides a versatile sensing strategy for intelligent diagnostics of different coronaviruses with low false-negative rates. © 2021 American Chemical Society
语种：
英文
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Integrated network analysis reveals that exogenous cadmium-tolerant endophytic bacteria inhibit cadmium uptake in rice
作者：
Zheng, Zhongyi;Li, Peng;Xiong, Ziqin;Ma, Tingting;Mathivanan, Krishnamurthy;...
期刊：
Chemosphere ,2022年301:134655 ISSN：0045-6535
通讯作者：
Li, Juan(adalee619@163.com);Rang, Zhongwen(rzwronger@126.com)
作者机构：
[Ao, Hejun; Rang, Zhongwen; Xiong, Ziqin; Zheng, Zhongyi; Li, Peng; Li, Juan; Ma, Tingting; Yi, Zhenxie] Hunan Agr Univ, Coll Agron, Changsha, Peoples R China.;[Praburaman, Loganathan; Meng, Delong; Mathivanan, Krishnamurthy] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha, Peoples R China.;[Wang, Qiming] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
通讯机构：
[Zhongwen Rang; Juan Li] C;College of Agronomy, Hunan Agricultural University, Changsha, China
关键词：
Cadmium-tolerant strains;Keystone species;Microbial interactions;Microbial networks;Rice cadmium content
摘要：
Most previous studies have focused on the diversity and species richness of microbial communities, however, understanding the interactions between species and detecting key functional members of the community can help us better understand how microorganisms perform their functions. In this study, the response of the rice plant microbial community to the inoculation of cadmium-resistant endophytic bacterium R5 (Stenotrophomonas) was investigated for the first time using a microbial phylogenetic molecular ecological network. The results showed that inoculation of R5 changed the topological characteristics of the microbial network in rice plants, with the resulting network displaying stronger complexity and interaction in roots and aboveground parts, indicating that inoculation of R5 provided favorable conditions for microbial interactions. In addition, these interactions may be related to the absorption and transportation of cadmium by rice. Under the exogenous addition of R5, the network interactions of the rice plant microbial community were more inclined to cooperation. Both in the roots and aboveground parts of rice, the plant Cd content showed a decrease as the complexity and connectivity of the network increased, suggesting that complex microbial networks may be more beneficial to rice than simple microbial networks because as they were more adaptive and resistant to unfavorable environments. After inoculation with the R5 strain, the negative interaction with Cd content in rice plants increased significantly, and there might be more synergy between the microbial community and plants to jointly inhibit the absorption and transportation of Cd. © 2022 Elsevier Ltd
语种：
英文
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Exploring the interaction between Cry1Ac protein and Zn2+, Cd2+ metal ions by fluorescence quenching and molecular docking approaches
作者：
Wang, Li;Liang, Yun-Shan;Wu, Zhi-Bin;Liu, Yi-Song;Xiao, Yun-Hua;...
期刊：
Chemosphere ,2022年297:134105 ISSN：0045-6535
通讯作者：
Liang, YS
作者机构：
[Gao, Rong; Liang, Yun-Shan; Wang, Li; Wu, Ai Ping; Hu, Teng; Liang, YS; Liu, Jiao; Wu, Zhi-Bin] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.;[Gao, Rong; Liang, Yun-Shan; Wang, Li; Wu, Ai Ping; Hu, Teng; Liu, Jiao; Wu, Zhi-Bin] Hunan Prov Key Lab Rural Ecosyst Hlth Dongting La, Changsha 410128, Peoples R China.;[Liu, Yi-Song] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.;[Liu, Yi-Song] Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha 410128, Peoples R China.;[Xiao, Yun-Hua; Fang, Jun] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Liang, YS ] H;Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Peoples R China.
关键词：
Binding mechanism;Cry1Ac protein;Fluorescence spectroscopy;Metal ions;Molecular docking
摘要：
Bacillus Thuringiensis (Bt) protein has a strong ability to complex with metal ions, which may increase the transport of metal ions in the soil multi-media system. In this study, the interactions between Cry1Ac protein and metal ions (Zn2+ and Cd2+) were investigated through spectroscopies and molecular docking methods. The spectra results showed that both Zn2+ and Cd2+ quenched the fluorescence intensity of Cry1Ac protein through the static quenching. The binding constants with 4–5 orders of magnitude also indicated the interactions between the ions and the Cry1Ac protein. The thermodynamic analysis showed that hydrogen bonds and van der Waals forces were predominant during the processes. In terms of the Förster non-radiation energy transfer theory, the binding distances between metal ions and Cry1Ac protein were approximately 0.21–0.24 nm, indicating the existence of a non-radiative energy transfer between them. Furthermore, molecular docking revealed that the metal ions participated in ligand binding with the Cry1Ac at the locations Asp569, Thr560, Asn564 and Gln566. The present work provided reasonable models helping us further understand the transport effect of heavy metals in the presence of Cry1Ac. The results could provide mechanistic insights into the nature of metal ions-Cry1Ac interactions and offer important information on the toxicity risk of metal ions-Cry1Ac binding interactions. © 2022 Elsevier Ltd
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英文
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Marginal land conversion to perennial energy crops with biomass removal enhances soil carbon sequestration
作者：
Xu, Yi;Zhou, Jie;Feng, Wenhao;Jia, Rong;Liu, Chunyan;...
期刊：
GCB Bioenergy ,2022年14(10):1117-1127 ISSN：1757-1693
通讯作者：
Zang, Huadong(zanghuadong@cau.edu.cn)
作者机构：
[Yang, Yadong; Zhou, Jie; Zang, Huadong; Zeng, Zhaohai; Xu, Yi; Jia, Rong; Feng, Wenhao; Liu, Chunyan] China Agr Univ, Coll Agron & Biotechnol, Beijing, Peoples R China.;[Xue, Shuai; Yi, Zili; Fu, Tongchen] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.;[Guillaume, Thomas] Field Crop Syst & Plant Nutr, Agroscope, Res Div Plant Prod Syst, Nyon, Switzerland.;[Peixoto, Leanne] Aarhus Univ, Dept Agroecol, Aarhus, Denmark.
通讯机构：
[Huadong Zang] C;College of Agronomy and Biotechnology, China Agricultural University, Beijing, China
关键词：
13C natural abundance;C3–C4 vegetation change;marginal land;Miscanthus;soil C sequestration;switchgrass
摘要：
Marginal land conversion to perennial energy crops increased soil C stocks. Switchgrass had a higher soil C sequestration potential than Miscanthus. New C4‐C input exceeds old C3‐C losses via mineralization leading to C sequestration. Abstract Marginal land conversion to perennial energy crops can provide biomass feedstocks and climate change mitigation. However, the effect of perennial energy crop cultivation on soil organic carbon (SOC) sequestration and its underlying mechanism in marginal land still remains incomplete. Here, SOC turnover, stability, and its potential sequestration were evaluated based on 10 years of land use change from C3 grass‐dominated marginal land to C4 energy crops Miscanthus and switchgrass cultivation. The naturally occurring 13C signature down to 60 cm depth was used to determine the energy crops‐derived C. Compared to reference marginal land, Miscanthus plantation increased the SOC stock at 0–60 cm depth by 17.8% and 64.3% in bulk and root zone, respectively. Similarly, the SOC stock under switchgrass was also 16.5% and 93.0% higher in bulk and root zone than in reference marginal land, respectively. The higher SOC stock in the root zone of switchgrass relative to Miscanthus was supported by the higher contribution of C4‐derived C to SOC (44.5% vs. 32.4%). The mean residence time of old C was higher under switchgrass than Miscanthus in the bulk zone across 0–60 cm (p < 0.05) but remained the same at 0–20 cm in the root zone. Specific SOC mineralization and temperature sensitivity were lower in soils under Miscanthus and switchgrass compared to reference marginal land. The partial least squares path model revealed that perennial energy crop cultivation enhances soil C stock via increased C4‐derived C input and reduced mineralization. In conclusion, marginal land conversion to perennial energy crops is a win–win strategy for C sequestration to mitigate climate change and support the growing bioenergy sector with biomass supply.
语种：
英文
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Elevated CO2 effect on the response of stomatal control and water use efficiency in amaranth and maize plants to progressive drought stress
作者：
Wei, Zhenhua;Abdelhakim, Lamis Osama Anwar;Fang, Liang;Peng, Xiaoying;Liu, Jie;...
期刊：
Agricultural Water Management ,2022年266:107609 ISSN：0378-3774
通讯作者：
Liu, Fulai(fl@plen.ku.dk)
作者机构：
[Wei, Zhenhua; Liu, Jie] Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas of Ministry of Education, Northwest A&F University, Shaanxi, Yangling;712100, China;[Abdelhakim, Lamis Osama Anwar; Fang, Liang; Liu, Fulai] Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegaard Allé 13, Taastrup;DK-2630, Denmark;[Peng, Xiaoying] College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan, Changsha
通讯机构：
[Fulai Liu] D;Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegaard Allé 13, DK-2630 Taastrup, Denmark
关键词：
C4 species;Drought;Elevated CO2;Plant growth;Stomatal control;Water use efficiency
摘要：
Rising CO2 concentration ([CO2]) in the atmosphere may modulate the response of crop plants to drought stress. This study aimed to investigate the response of leaf gas exchange and plant growth of two C4 species representing both dicot (amaranth) and monocot (maize) to progressive drought under two different [CO2] (ambient (a[CO2], 400 ppm) and elevated (e[CO2], 800 ppm)). The soil water status in the pots was expressed as the fraction of transpirable soil water (FTSW). The results showed that as compared to a[CO2], e[CO2] significantly increased net photosynthetic rate (An) at non-stress condition (An max) for both species, while the increase was more pronounced in maize than in amaranth. Stomatal conductance (gs) at non-stress condition was significantly lower at e[CO2] in both species. The FTSW threshold, at which An starts to decrease, was higher in maize grown at e[CO2] than at a[CO2], whereas it was not affected in amaranth. In both species, gs decreased at higher FTSW threshold when grown at e[CO2] than at a[CO2]. e[CO2] decreased stomatal density (SD) in amaranth but increased it in maize; drought increased SD in amaranth but not in maize. Intrinsic water use efficiency (WUEi) was significantly enhanced by e[CO2] and drought stress at FTSW ranged from 0.0 to 0.6, particularly in maize. e[CO2] increased leaf area of well-watered plants in maize and decreased specific leaf area in amaranth. In amaranth, water consumption of well-watered plants was increased and plant WUE was decreased at e[CO2]. The varied responses of leaf gas exchange and WUE to soil water deficits and e[CO2] among the two C4 species imply their different mechanisms in stomatal control over carbon gain versus water loss in dicot and monocot plants, which is essentially important for selecting crop species and developing strategies to optimize crop WUE in a future drier and CO2-enriched climate. The more physiological and biochemical response from soil, stomatal to plant scale related to various environments would be considered in further investigation. © 2022 The Authors
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英文
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Defective polymeric carbon nitride: Fabrications, photocatalytic applications and perspectives
作者：
Yang, Jinjuan;Wang, Hou;Jiang, Longbo;Yu, Hanbo;Zhao, Yanlan;...
期刊：
Chemical Engineering Journal ,2022年427 ISSN：1385-8947
通讯作者：
Jiang, LB
作者机构：
[Jiang, Longbo; Yang, Jinjuan; Zhao, Yanlan; Chen, Haoyun; Yuan, Xingzhong; Wang, Hou; Yu, Hanbo; Liang, Jie] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Peoples R China.;[Yang, Jinjuan; Zhao, Yanlan; Chen, Haoyun; Yuan, Xingzhong; Wang, Hou; Yu, Hanbo; Liang, Jie; Jiang, Longbo] Hunan Univ, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Peoples R China.;[Jiang, Longbo] Hong Kong Polytech Univ, Dept Civil & Environm Engn, Hung Hom, Hong Kong, Peoples R China.;[Li, Hui] Hunan Acad Forestry, Inst Biol & Environm Engn, Changsha 410004, Peoples R China.;[Wu, Zhibin] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Jiang, LB ] H;Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Peoples R China.
关键词：
Active sites;Band structure;Defect engineering;Photocatalytic;Polymeric carbon nitride;Vacancies
摘要：
Polymeric carbon nitride (CN) as a metal-free photocatalyst with suitable band gap has attracted increasing attention. However, pristine CN has a limited visible-light absorption range and also suffers a high recombination rate of photo-excited charge carriers, resulting in low photocatalytic activity. With the development of modern material characterization technology and electronic structure calculation technology, it is found that vacancy defects in CN play a more decisive role in determining the kinetics, energetics and mechanism of photocatalytic reactions. Therefore, much effort has been made in the past years to enhance the visible light activity of CN through defects control. This review provides the recent progresses and technologies on fabricating defective CN, in which the bottom-up and top-down preparation strategies are systematically summarized. In the meantime, the advancement of defective CN photocatalysts toward versatile applications such as water oxidation, hydrogen production, CO2 reduction, nitrogen fixation, H2O2 and organic synthesis, and pollutants removal are fully discussed. Finally, the existing opportunities and challenges of CN photocatalysts designed with vacancies defects are proposed to highlight the development direction of this field. This paper can lay a theoretical basis and guidance for the preparation of defective graphitic carbon nitride. © 2021 Elsevier B.V.
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英文
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Long-term cultivation of Miscanthus and switchgrass accelerates soil organic carbon accumulation by decreasing carbon mineralization in infertile red soil
作者：
Fu, Tongcheng;Xu, Yi;Hou, Wei;Yi, Zili;Xue, Shuai
期刊：
GCB Bioenergy ,2022年14(9):1065-1077 ISSN：1757-1693
通讯作者：
Xue, Shuai(xue_shuai@hunau.edu.cn)
作者机构：
[Xue, Shuai; Hou, Wei; Yi, Zili; Fu, Tongcheng] Hunan Agr Univ, Coll Biosci & Amp Biotechnol, Changsha, Peoples R China.;[Xue, Shuai; Hou, Wei; Yi, Zili; Fu, Tongcheng] Hunan Agr Univ, Hunan Engn Lab Miscanthus Ecol Applicat, Changsha, Peoples R China.;[Xue, Shuai; Hou, Wei; Yi, Zili; Fu, Tongcheng] Hunan Agr Univ, Natl Energy R&D Ctr Nonfood Biomass, Hunan Branch, Changsha, Peoples R China.;[Xu, Yi] China Agr Univ, Coll Agron & Biotechnol, Beijing, Peoples R China.
通讯机构：
[Shuai Xue; Shuai Xue Shuai Xue Shuai Xue] C;College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha, PR China<&wdkj&>Hunan Engineering Laboratory of Miscanthus Ecological Applications, Hunan Agricultural University, Changsha, PR China<&wdkj&>Hunan Branch, National Energy R & D Center for Non-food Biomass, Hunan Agricultural University, Changsha, PR China
关键词：
C4 energy crop;enzyme stoichiometry;marginal land;perennial grass;phytoremediation;soil organic carbon stock
摘要：
Perennial energy crops (PECs) such as Miscanthus spp. and switchgrass (Panicum virgatum L.) may have particular influence on microbial communities and their functions in soil organic carbon (C) utilization and mineralization (Cm). In this study, long-term effects of PECs on Cm and soil hydrolase activities were examined in bulk and rhizosphere soils of Miscanthus and switchgrass grown on a red soil in South China. Long-term cultivation (10 years) of PECs led to increases in soil organic C (SOC) and the absolute Cm of bulk and rhizosphere soils. Total Cm was correlated with dissolved organic C and dissolved organic nitrogen (N) in red soils. The specific Cm in bulk soils of switchgrass and Miscanthus were decreased by 11.73% and 20.67% comparing with the control group, respectively. Cultivation of PECs led to a relatively high C/N ratio. There was a difference in priming effect on activities of β-glucosidase (BG), leucine amin peptidase, N-acetyl-β-glucosaminidase (NAG), and phosphatase between rhizosphere and bulk soils. Compared with the control without PECs, BG activity did not change significantly in bulk soils of PECs, whereas NAG activity decreased significantly. Soil microorganisms were generally limited by soil C and phosphorus (P) in the red soil. The C and P limitations were significantly linearly related with relative Cm (p < 0.05). Phosphorus limitations in microbial communities were lower with PECs than in the control, indicating that cultivation of PECs could provide an optimal nutrient environment for both plants and microorganisms. Thus, long-term cultivation of PECs increased SOC content but decreased specific Cm rates. However, because C and P limitations remain for plants and soil microbial communities, optimum fertilization is also necessary for sustainable growth of PECs on red soils. © 2022 The Authors. GCB Bioenergy published by John Wiley & Sons Ltd.
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英文
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Elucidating the Differences in Metal Toxicity by Quantitative Adverse Outcome Pathways
作者：
Yang, Lanpeng;Zeng, Jing;Gao, Ning;Zhu, Lin;Feng, Jianfeng
期刊：
Environmental Science & Technology ,2022年56(18):13233-13244 ISSN：0013-936X
通讯作者：
Feng, Jianfeng(fengjf@nankai.edu.cn)
作者机构：
[Gao, Ning; Zhu, Lin; Feng, Jianfeng; Yang, Lanpeng] Nankai Univ, Key Lab Pollut Proc & Environm Criteria, Minist Educ, Tianjin 300071, Peoples R China.;[Gao, Ning; Zhu, Lin; Feng, Jianfeng; Yang, Lanpeng] Nankai Univ, Tianjin Key Lab Environm Remediat & Pollut Control, Coll Environm Sci & Engn, Tianjin 300071, Peoples R China.;[Zeng, Jing] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410004, Peoples R China.
通讯机构：
[Jianfeng Feng] K;Key Laboratory of Pollution Process and Environmental Criteria of Ministry of Education and Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, P. R. China
关键词：
quantitative adverse outcome pathway;zebrafish larvae;metals;toxicokinetic;toxicodynamic;toxicity difference
摘要：
Numerous studies have reported that the toxicity differences among metals are widespread; however, little is known about the mechanism of differences in metal toxicity to aquatic organisms due to the lack of quantitative understanding of their adverse outcome pathway. Here, we investigated the effects of Cd and Cu on bioaccumulation, gene expression, physiological responses, and apical effects in zebrafish larvae. RNA sequencing was conducted to provide supplementary mechanistic information for the effects of Cd and Cu exposure. On this basis, we proposed a quantitative adverse outcome pathway (qAOP) suitable for metal risk assessment of aquatic organisms. Our work provides a mechanistic explanation for the differences in metal toxicity where the strong bioaccumulation of Cu enables the newly accumulated Cu to reach the threshold that causes different adverse effects faster than Cd in zebrafish larvae, resulting in a higher toxicity of Cu than that of Cd. Furthermore, we proposed a parameter CIT/BCF (the ratio of internal threshold concentration and bioaccumulation factor) that helps to understand the toxicity differences by combining the information of bioaccumulation and internal threshold of adverse effects. This work demonstrated that qAOP is an effective quantitative tool for understanding the toxicity mechanism and highlight the importance of toxicokinetics and toxicodynamics at different biological levels in determining the metal toxicity. © 2022 American Chemical Society.
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英文
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Arsenic biotransformation genes and As transportation in soil-rice system affected by iron-oxidizing strain (Ochrobactrum sp.)
作者：
Xue, Shengguo;He, Xuan;Jiang, Xingxing;Pan, Weisong;Li, Waichin;...
期刊：
Environmental Pollution ,2022年314:120311 ISSN：0269-7491
通讯作者：
Wu, Chuan(wuchuan@csu.edu.cn)
作者机构：
[Xue, Shengguo; He, Xuan; Wu, Chuan; Jiang, Xingxing] Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.;[Wu, Chuan; Li, Waichin] Educ Univ Hong Kong, Dept Sci & Environm Studies, Hong Kong, Peoples R China.;[Pan, Weisong; Xia, Libing] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Chuan Wu] S;School of Metallurgy and Environment, Central South University, Changsha, 410083, PR China<&wdkj&>Department of Science and Environmental Studies, The Education University of Hong Kong, Administrative Region, Hong Kong, PR China
关键词：
Arsenic biotransformation gene;Ferrous iron oxidation;Iron oxidizing strain;Soil-rice system
摘要：
Arsenic (As) biotransformation in soil affects As biogeochemical cycling and is associated with As accumulation in rice. After inoculation with 1% iron-oxidizing bacteria (FeOB) in paddy soil, As speciation, As biotransformation genes in soil, As/Fe in Fe plaques, and As accumulation in rice were characterized. Compared with the control, the available As concentrations in soils decreased while amorphous and poorly crystalline Fe-Al oxidized As and crystalline Fe-Al oxidized As fractions increased of F (FeOB) and RF (rice and FeOB) treatments. Fe concentrations increased and positively correlated with As concentrations in Fe plaques on the rice root surface (***P<0.001). Compared with R (rice), Monomethyl As (MMA), dimethyl As (DMA), arsenate (As(V)), and arsenite (As(III)) concentrations in rice plants showed a downwards trend of RF treatment. The As concentration in grains was below the National Standard for Food Safety (GB 2762-2017). A total of 16 As biotransformation genes in rhizosphere soils of different treatments (CK, F, R and RF were quantified by high-throughput qPCR (HT-qPCR). Compared with the control, the As(V) reduction and As transport genes abundance in other treatments increased respectively by 54.54%-69.17% and 54.63%-73.71%; the As(III) oxidation and As (de) methylation genes did not change significantly; however, several As(III) oxidation genes (aoxA, aoxB, aoxS, and arsH) increased. These results revealed that FeOB could reduce, transport As, and maybe also oxidize As. In addition, As(III) oxidation gene (aoxC) in rhizosphere soil was more abundant than in non-rhizosphere soil. It indicated that radial oxygen loss (ROL) promoted As(III) oxidation in rhizosphere soils. The results provide evidence for As biotransformation by ROL and FeOB in soil-rice system. ROL affects As oxidation and immobilization, and FeOB affects As reduction, transportation and may also affect As oxidation.
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Bioenergy Production Potential of 16 Energy Crops on Marginal Land in China
作者：
Fu, Tongcheng;Xu, Yi;Li, Meng;Xue, Shuai;Duan, Zengqiang;...
期刊：
BioEnergy Research ,2022年15(2):998-1016 ISSN：1939-1234
通讯作者：
Xie, Guang Hui
作者机构：
[Xue, Shuai; Li, Meng; Fu, Tongcheng; Xu, Yi] Hunan Agr Univ, Coll Biosci Sr Biotechnol, Changsha 410128, Peoples R China.;[Xue, Shuai; Li, Meng; Fu, Tongcheng; Xu, Yi; Xie, Guang Hui] China Agr Univ, Natl Energy R&D Ctr Nonfood Biomass, Beijing 100193, Peoples R China.;[Duan, Zengqiang] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China.;[Xie, Guang Hui] China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China.
通讯机构：
[Xie, Guang Hui] C;China Agr Univ, Natl Energy R&D Ctr Nonfood Biomass, Beijing 100193, Peoples R China.;China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China.
关键词：
Energy crop;Marginal land;Energy demand;Bioelectrogenesis;Biodiesel;Bioethanol;GIS
摘要：
Alternative energy sources, derived from biomass feedstocks, are important for China’s energy security. To evaluate China’s capacity for bioenergy in the future, Geographic Information System (GIS) techniques were adopted to identify potentially productive marginal areas for the growth of 16 energy crops and to comprehensively estimate their biomass and bioenergy production potential under three scenarios. The results showed that a total of 267.23 Mha (i.e., million hectare) of marginal land was available for energy crop production. When assessing the suitability of individual environments for each type of energy crop, the largest total land area of a lignocellulosic energy crop, an oil energy crop, and a lignocellulosic energy tree were 3.64 Mha (Miscanthus spp.), 45.44 Mha (Jatropha curcas L.), and 63.55 Mha [Haloxylon ammodendron (C. A. Mey.) Bunge], respectively. In addition, the proposed production of bioethanol [Sorghum bicolor (L.) Moench], biodiesel [Swida wilsoniana (Wanger.) Sojak], and bio-electricity [H. ammodendron] in the baseline scenario met 31.83%, 26.08%, and 9.2% of ethanol demand for E10, diesel, and electricity in 2016, respectively. Furthermore, if upland and forest land could be used to produce bioenergy, the bioethanol substitution rates of ethanol for E100, diesel, and electric power were calculated as 62.63%, 153.94%, and 60.47%, respectively, for 2040. The results indicated that China’s future energy demands could not be satisfied by simply depending on energy crops in most situations. Future bioenergy should be developed based on reliable supplies of several types of biomass materials and dedicated biochemical or thermochemical conversion processes, among other renewable energy sources. Graphical abstract: [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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Heterotrophic ammonium assimilation: An important driving force for aerobic denitrification of Rhodococcus erythropolis strain Y10
作者：
Ma, Shu;Huang, Shiwei;Tian, Yun;Lu, Xiangyang
期刊：
Chemosphere ,2022年291 ISSN：0045-6535
通讯作者：
Lu, XY
作者机构：
[Tian, Yun; Lu, Xiangyang; Ma, Shu; Huang, Shiwei; Lu, XY] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Lu, XY ] H;Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
关键词：
Aerobic denitrification;Heterotrophic ammonium assimilation;Rhodococcus erythropolis;Wastewater biological nitrogen removal
摘要：
Studies on microbial ammonium removal have focused on the heterotrophic nitrification of microorganisms and have rarely studied the role of ammonium assimilation. In this study, Rhodococcus erythropolis strain Y10 with the capacity of aerobic denitrification was screened from the surface flow constructed wetlands that treat high-strength ammonium swine wastewater. Instead of through nitrification, this strain removed ammonium through heterotrophic ammonium assimilation, with the removal rate of 9.69 mg/L/h. The KEGG nitrogen metabolism pathway analysis combined with nitrogen balance calculation manifested that the removal of nitrate and nitrite by R. erythropolis Y10 was achieved through two pathways: 1) assimilation reduction to biomass nitrogen and 2) aerobic denitrification reduction to gaseous nitrogen. Ammonium addition improved the aerobic denitrification rate of nitrate and nitrite. The maximal reduction rates of nitrate and nitrite increased from 7.82 and 7.23 mg/L/h to 9.09 and 8.09 mg/L/h respectively, when 100 mg/L ammonium was separately added to 150 mg/L nitrate and nitrite. Furthermore, the removal efficiency of total nitrogen increased from 69.80% and 77.65% to 89.19% and 91.88%, respectively. Heterotrophic ammonium assimilation promoted the aerobic denitrification efficiency of Rhodococcus erythropolis strain Y10. © 2021 Elsevier Ltd
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Mycorrhizal Benefits of Salt-Stressed Cinnamomum camphora (L.) Presl. May Be Related to P and Mn2+ Contents in Shoots, Biomass Allocation, and K+/Na+ in Roots and Shoots
作者：
Wang, Yanhong;Li, Tiantian;Wu, Aiping;Li, Yan;Zhang, Naili
期刊：
Forests ,2022年13(11):1882- ISSN：1999-4907
通讯作者：
Wang, Yanhong(wangyanhong@zafu.edu.cn);Zhang, Naili(zhangnaili@bjfu.edu.cn)
作者机构：
[Li, Tiantian; Li, Yan; Wang, Yanhong] Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China.;[Wu, Aiping] Hunan Agr Univ, Coll Resources & Environm, Ecol Dept, Hunan Prov Key Lab Rural Ecosyst Hlth Dongting La, Changsha 410128, Peoples R China.;[Zhang, Naili] Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing 100083, Peoples R China.
通讯机构：
[Yanhong Wang] A;[Naili Zhang] T;Authors to whom correspondence should be addressed.<&wdkj&>State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China<&wdkj&>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China<&wdkj&>Authors to whom correspondence should be addressed.
关键词：
Cinnamomum camphora;Funneliformis mosseae;K+/Na+;P and Mn2+ concentrations;Rhizophagus irregularis;salinity
摘要：
Arbuscular mycorrhizal fungi (AMF) are taken as bioameliorators to alleviate the detrimental effects of salt stress. However, how AMF affect the performance of Cinnamomum camphora, an economically important species, remains unclear. In this study, we evaluated the interactive effects of AMF and salinity on the growth, nutrient acquisition, and ion ratios of C. camphora. A factorial experiment was implemented in a greenhouse with four fungal regimes (inoculation with sterilized AMF or with Funneliformis mosseae or Rhizophagus irregularis either alone or in combination), and three salt regimes (0, 50, and 200 mM NaCl). Results showed that salinity alone significantly reduced the total dry weight, mycorrhizal colonization, K+ concentration, and ionic homeostasis (particularly K+:Na+, Mg2+:Na+, and Ca2+:Na+) of whole plants. Mycorrhizal inoculation, particularly with R. irregularis, strongly mitigated some of the detrimental effects of salinity, enhancing the salt tolerance of C. camphora. Furthermore, the host plants benefited from the presence of AMF, mainly because they enhanced P and Mn2+ concentrations in the shoots, adjusted biomass allocation, and shifted the selective transporting capacity of K+ over Na+ from roots to shoots. Our results suggested that building mycorrhizal association between C. camphora and R. irregularis may be useful for plant cultivation in coastal areas. © 2022 by the authors.
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Fluorescence thermometers: intermediation of fundamental temperature and light
作者：
Feng, Guangfu;Zhang, Huaizu;Zhu, Xiaohua;Zhang, Jiaheng;Fang, Jun
期刊：
BIOMATERIALS SCIENCE ,2022年10(8):1855-1882 ISSN：2047-4830
通讯作者：
Fang, J;Zhang, JH
作者机构：
[Feng, Guangfu; Zhang, Huaizu; Fang, Jun] Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;[Zhu, Xiaohua] Hunan Agr Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China.;[Zhang, Jiaheng] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
通讯机构：
[Fang, J ; Zhang, JH ] H;Hunan Agr Univ, Sch Biosci & Biotechnol, Changsha 410128, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
摘要：
The rapid advancement of thermal materials and fluorescence spectroscopy has extensively promoted the development of micro-scale fluorescence thermometry in recent years. Based on their advantages of fast response, high sensitivity, simple operation, high spatial resolution, and non-destructive detection, fluorescence thermometers have become powerful analysis tools used to sense temperature fluctuations through fluorescent signals, especially to accurately capture living cells via fluorescent signals and local temperature variations in living bodies, thus providing the most direct means for the in-depth understanding of biological processes in cells. Herein, we systematically categorize the currently reported fluorescence thermometers based on the aspects of fluorescence intensity and wavelength, reveal the intrinsic relationship between fluorescence (intensity and wavelength) and temperature response, expound the applications of fluorescence thermometers in the fields of chemical sensing and biomedicine, and analyze the challenges faced by current fluorescence thermometers based on fundamental problems and practical applications simultaneously, thus highlighting the future directions of fluorescence thermometers. © 2022 The Royal Society of Chemistry.
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英文
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Synthesis, characterization and absorption evaluation of bifunctional monomer magnetic molecularly imprinted polymers nanoparticles for the extraction of 6-benzylaminopurine from vegetables
作者：
Xiong, Yingzi;Cao, Yanan;Li, Pao;Li, Maiquan;Wang, Ruozhong;...
期刊：
Food Chemistry ,2022年386:132792 ISSN：0308-8146
通讯作者：
Xiao, Langtao(ltxiao@hunau.edu.cn)
作者机构：
[Cao, Yanan; Li, Maiquan; Xiong, Yingzi; Liu, Xia; Li, Pao] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Peoples R China.;[Wang, Ruozhong; Xiao, Langtao] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.
通讯机构：
[Xiao, L.] H;Hunan Provincial Key Laboratory of Phytohormones and Growth Development, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China
关键词：
6-Benzylaminopurine;Bifunctional monomers;Extraction;Magnetic separation;Molecularly imprinted polymer;Vegetables
摘要：
An adsorbent-magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) were synthesized for the extraction of 6-benzylaminopurine (6-BA) using Fe3O4 as magnetic core. The MIPs were prepared with methacrylic acid and sodium p-styrene sulfonate as bifunctional monomers. The adsorbents were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffractometer, thermogravimetric analysis and vibrating sample magnetometer. The adsorption properties were evaluated by static, kinetic and selective adsorption experiments. The MMIPs NPs exhibit a high adsorption capacity (37.63 mg g−1) and favorable imprinting factor (2.88) toward 6-BA. The chromatogram of 6-BA extraction using the MMIPs NPs as the adsorbent demonstrates that the matrix interference has been minimized. More importantly, MMIPs NPs can be applied to extracting 6-BA from mung bean sprout and cucumber with satisfactory recoveries (91.14–104.52%), and can be reused for at least five times. This work provides a new strategy to efficiently extract 6-BA from vegetables. © 2022 Elsevier Ltd
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Advances in physiological functions and mechanisms of (-)-epicatechin
作者：
Qu, Zhihao;Liu, Ailing;Li, Penghui;Liu, Changwei;Xiao, Wenjun;...
期刊：
Critical Reviews in Food Science and Nutrition ,2021年61(2):211-233 ISSN：1040-8398
通讯作者：
Liu, Zhonghua;Zhang, Sheng
作者机构：
[Liu, Changwei; Liu, Zhonghua; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan; Qu, Zhihao] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.;[Liu, Changwei; Liu, Zhonghua; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan; Qu, Zhihao] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Collaborat Innovat Ctr Utilisat Funct Ingredients, Changsha, Hunan, Peoples R China.;[Liu, Ailing] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, ZH; Zhang, S] H;Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China.
关键词：
(-)-epicatechin;Antidiabetic;anti-inflammatory;anti-oxidant;cardiovascular disease;muscle atrophy;neurodegenerative disease
摘要：
(-)-Epicatechin (EC) is a flavanol easily obtained through the diet and is present in tea, cocoa, vegetables, fruits, and cereals. Recent studies have shown that EC protects human health and exhibits prominent anti-oxidant and anti-inflammatory activities, enhances muscle performance, improves symptoms of cardiovascular and cerebrovascular diseases, prevents diabetes, and protects the nervous system. With the development of modern medical and biotechnology research, the mechanisms of action associated with EC toward various chronic diseases are becoming more apparent, and the pharmacological development and utilization of EC has been increasingly clarified. Currently, there is no comprehensive systematic introduction to the effects of EC and its mechanisms of action. This review presents the latest research progress and the role of EC in the prevention and treatment of various chronic diseases and its protective health effects and provides a theoretical basis for future research on EC.
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英文
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Environmental and spatial drivers for wetland plant communities in a freshwater lake: Reduced coupling of species and functional turnover
作者：
Fu, Hui* ;Zhou, Ye;Yuan, Guixiang;Peng, Hui;Wu, Aiping;...
期刊：
Ecological Engineering ,2021年159:106092 ISSN：0925-8574
通讯作者：
Fu, Hui
作者机构：
[Wu, Aiping; Zhou, Ye; Fu, Hui; Yuan, Guixiang; Peng, Hui] Hunan Agr Univ, Dept Ecol, Coll Resources & Environm, Hunan Prov Key Lab Rural Ecosyst Hlth Dongting La, Changsha 410128, Peoples R China.;[Li, Wei] Nanchang Inst Technol, Res Inst Ecol & Environm Sci, Nanchang 330099, Jiangxi, Peoples R China.;[Jeppesen, Erik] Aarhus Univ, Dept Biosci, Vejlsovej 25, DK-8600 Silkeborg, Denmark.;[Jeppesen, Erik] Univ Chinese Acad Sci, Sino Danish Ctr Educ & Res SDC, Beijing, Peoples R China.;[Jeppesen, Erik] Middle East Tech Univ, Dept Biol Sci, Limnol Lab, Ankara, Turkey.
通讯机构：
[Fu, Hui] H;Hunan Agr Univ, Dept Ecol, Coll Resources & Environm, Hunan Prov Key Lab Rural Ecosyst Hlth Dongting La, Changsha 410128, Peoples R China.
关键词：
Beta diversity;Decoupling of species and functions;Functional redundancy;Poyang Lake wetland;Spatial scale
摘要：
Disentangling the contributions of environmental gradients and spatial distance as species distribution drivers is a crucial topic of community ecology. Here, we sampled wetland plant communities at 779 plots from 52 sites and measured 12 key functional traits of 52 plant species in Poyang Lake, China. Using the generalized dissimilarity model (GDM), we examined which environmental variables and geographic distances best predicted the variations in community turnover (Simpson dissimilarity coefficients) at two spatial scales (i.e., plot and site) and compared the turnover patterns between species and functional approaches. We found that both species and functional turnover were typically higher at plot than site scale and that species turnover were higher than functional turnover across the studied scales. At plot scale, the GDM results showed that the best predictor of species turnover was elevation followed by soil moisture and geographic distance, while geographic distance was the best predictor at site scale. However, functional turnover was weak or showed no remarkable responses to either environmental variables or geographic distance. Notably, the communities showed a very high functional redundancy with slow shifts across the environmental gradients at both scales studied. This strongly suggests that most species were functionally redundant and that species replacement across environmental gradients and the two studied spatial scales is restricted to functionally identical species in the Poyang Lake wetland. Our results demonstrated that the coupling between plant species and functional turnover is reduced, which may allow the wetland plant community to maintain its ecosystem functioning when subjected to water level fluctuations. © 2020 Elsevier B.V.
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英文
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Stimuli-Responsive Autonomous-Motion Molecular Machine for Sensitive Simultaneous Fluorescence Imaging of Intracellular MicroRNAs
作者：
Xue, Chang;Luo, Mengxue;Wang, Lei;Li, Congcong;Hu, Shuyao;...
期刊：
Analytical Chemistry ,2021年93(28):9869-9877 ISSN：0003-2700
通讯作者：
Wu, Zai-Sheng(wuzaisheng@163.com)
作者机构：
[Yu, Xin; Li, Congcong; Xue, Chang; Hu, Shuyao; Luo, Mengxue; Yuan, Pei; Wang, Lei; Wu, Zai-Sheng] Fuzhou Univ, Coll Chem Engn, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Canc Metastasis Alert & Prevent Ctr,Fujian Prov K, Fuzhou 350108, Peoples R China.;[Wang, Lei] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.
通讯机构：
[Zai-Sheng Wu] C;College of Chemical Engineering, Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350108, China
摘要：
DNAzymes with enzymatic activity identified from random DNA pools by in vitro selection have recently attracted considerable attention. In this work, a DNAzyme-based autonomous-motion (AM) molecular machine is demonstrated for sensitive simultaneous imaging of different intracellular microRNAs (miRNAs). The AM molecular machine consists of two basic elements, one of which is a target-analogue-embedded double-stem hairpin substrate (TDHS) and the other is a locking-strand-silenced DNAzyme (LSDz). LSDz can be activated by target miRNA and catalytically cleave TDHS, generating Clv-TDHS and releasing free target analogue capable of triggering the next round of cleavage reaction. As such, the molecular machine can exert sustainable autonomous operation, producing an enhanced signal. Because the active target analogue comes from the machine itself and offers cyclical stimulation in a feedback manner, this target-induced autonomous cleavage circuit is termed a self-feedback circuit (SFC). The SFC-based molecular machine can be used to quantify miRNA-21 down to 10 pM without interference from nontarget miRNAs, indicating a substantial improvement in assay performance compared with its counterpart system without an SFC effect. Moreover, due to the enzyme-free process, the AM molecular machine is suitable for miRNA imaging in living cells, and the quantitative results are consistent with the gold standard PCR assay. More interestingly, the AM molecular machine can be used for the simultaneous fluorescence imaging of several intracellular miRNAs, enabling the accurate discrimination of cancerous cells (e.g., HeLa and MCF-7) from healthy cells. The SFC-based autonomous-motion machine is expected to be a promising tool for the research of molecular biology and early diagnosis of human diseases. © 2021 American Chemical Society.
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英文
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Core-shell Ag@nitrogen-doped carbon quantum dots modified BiVO4 nanosheets with enhanced photocatalytic performance under Vis-NIR light: Synergism of molecular oxygen activation and surface plasmon resonance
作者：
Zhang, Jin;Si, Mengying;Jiang, Longbo* ;Yuan, Xingzhong* ;Yu, Hanbo;...
期刊：
Chemical Engineering Journal ,2021年410:128336 ISSN：1385-8947
通讯作者：
Jiang, Longbo;Yuan, Xingzhong
作者机构：
[Guo, Jiayin; Zhang, Jin; Yuan, Xingzhong; Yu, Hanbo; Li, Yifu; Jiang, Longbo; Yuan, XZ] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.;[Guo, Jiayin; Zhang, Jin; Yuan, Xingzhong; Yu, Hanbo; Li, Yifu; Jiang, Longbo] Hunan Univ, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Peoples R China.;[Zhang, Jin; Si, Mengying] Changsha Univ Sci & Technol, Sch Chem & Food Engn, Changsha 410114, Peoples R China.;[Si, Mengying] Cent South Univ, Sch Met & Environm, Changsha 410083, Peoples R China.;[Wu, Zhibin] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构：
[Jiang, LB; Yuan, XZ] H;Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China.
关键词：
Ag@N-CQDs core–shell structures;BiVO4;Photocatalysis;Vis-NIR light
摘要：
Visible-near infrared (Vis-NIR) light accounts for ~95% of solar light. In recent years, rational design of Vis-NIR responsive photocatalyst with improved photocatalytic performance has attracted growing concern. In this work, a Vis-NIR responsive photocatalyst was developed by integrating core–shell structured Ag@nitrogen-doped carbon quantum dots (N-CQDs) with BiVO4 nanosheets. The efficiencies of tetracycline hydrochloride (TC) removal based on the BiVO4/Ag@N-CQDs composite were 80.37% and 65.67% under visible light irradiation (λ > 420 nm) for 60 min and NIR light irradiation (λ > 760 nm) for 180 min, respectively. This improved photocatalytic performance of BiVO4/Ag@N-CQDs composite stems from the synergistic effect between BiVO4 nanosheets and core–shell structured Ag@N-CQDs nanoparticles. The as-prepared BiVO4/Ag@N-CQDs composite exhibits surface plasmon resonance (SPR) and molecular oxygen activation. Thus, the charge separation, light absorption in Vis-NIR region and oxidation ability are boosted during the photocatalytic activity. This work provides a new insight for designing advanced structure of Vis-NIR responsive photocatalyst. © 2020 Elsevier B.V.
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