Structural Characterization and Immunostimulatory Activity of Heteropolysaccharides from Fuzhuan Brick Tea
作者:
Chen, Guijie;Bai, Yixun;Zeng, Ziqi;Peng, Yujia;Zhou, Wangting;...
期刊:
Journal of Agricultural and Food Chemistry ,2021年69(4):1368-1378 ISSN:0021-8561
通讯作者:
Zeng, Xiaoxiong;Liu, Zhonghua
作者机构:
[Chen, Guijie; Zeng, Ziqi; Peng, Yujia; Zeng, Xiaoxiong; Bai, Yixun; Zhou, Wangting] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;[Shen, Wenbiao] Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Key Lab Minist Educ Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Zeng, Xiaoxiong; Liu, Zhonghua] N;[Liu, Zhonghua] H;Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;Hunan Agr Univ, Key Lab Minist Educ Tea Sci, Changsha 410128, Hunan, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.
关键词:
Fuzhuan brick tea;polysaccharides;structural characterization;immunostimulatory activity
摘要:
Fuzhuan brick tea (FBT), one of the unique dark teas, has various health-promoting functions. In the present study, one polysaccharide fraction, namely FBTPS-2-1, was extracted and purified from FBT, and its structure and potential immunostimulatory activity were investigated. The results showed that FBTPS-2-1,one of typical heteropolysaccharides, was mainly composed of Gal, Ara, and Glc with little molar content of Man, Rha, GalA, and GlcA in molar ratio of 46.59:22.13:13.57:8.20:6.02:2.12:1.38 and molecular weight of 748 kDa. The backbone of FBTPS-2-1 contained -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Galp-(1 ->, -> 4)-beta-D-Galp-(1 -> 4)-alpha-D-Glcp-(1 ->, -> 4)-alpha-D-Glcp-(1 -> 4)-alpha-D-Glcp- (1 ->, -> 4)-alpha-D-Glcp-(1 -> 4)-beta-D-Galp-(1 ->, -> 3)-beta-D-Galp-(1 -> 4)-beta-D-Galp-(1 ->, -> 3,6)-beta-D-Galp- (1 -> 3)-beta-D-Galp- (1 -> and -> 3,6)-beta-D-Galp-(1 -> 3,6)-beta-D-Galp-(1 ->. The linkages of branches in FBTPS-2-1 were mainly composed of alpha-L-Araf-(1 -> 3,6)-beta-D-Galp-(1 ->, -> 5)-alpha-L-Araf-(1 -> 3,6)-beta-D-Galp-(1 ->, -> 6)-beta-D-Galp41 -> 3,6)-beta-D-Galp-(1 ->, alpha-L-Araf-(1 -> 3,S)-alpha-L-Araf-(1 ->, -> 3,5)-alpha-L-Araf- (1 -> 5)-alpha-L-Araf-(1 ->, alpha-D-Galp-(1->3,5)-alpha-L-Araf-(1 -> and -> 5)-alpha-L-Araf-(1 -> 6)-beta-D-Galp-(1 ->. Furthermore, FBTPS-2-1 could increase the phagocytosis of macrophages and promote the secretion of NO and a variety of inflammatory cytokines, including TNF-alpha, IL-1 beta, and IL-6, indicating noticeable immune enhancement activity. Thus, FBTPS-2-1 could serve as a potentially functional food to improve human health by modulating the host immunoreaction.
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英文
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Cold Stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis
作者:
Zhao, Mingyue;Cai, Binbin;Jin, Jieyang;Zhang, Na;Jing, Tingting;...
期刊:
园艺学报(英文版) ,2020年6(6):439-449 ISSN:2095-9885
通讯作者:
Song, Chuankui
作者机构:
[Jing, Tingting; Wang, Jingming; Zhao, Yifan; Li, Yating; Jin, Jieyang; Cai, Binbin; Zhang, Na; Zhao, Mingyue; Song, Chuankui; Pan, Yuting; Zhang, Mengting; Feng, Yingying; Yu, Feng; Zhou, Zixiang] Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Int Joint Lab Tea Chem & Hlth Effects, Hefei 230036, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Key Lab Tea Sci,Minist Educ, Changsha 410128, Peoples R China.
通讯机构:
[Song, Chuankui] A;Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Int Joint Lab Tea Chem & Hlth Effects, Hefei 230036, Peoples R China.
关键词:
Camellia sinensis;Eugenol;Glucosyltransferase;Cold stress
摘要:
Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants. Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality. However, the details of their biosynthesis and metabolism in tea plants are still unknown. Here, eugenol glucoside was unambiguously identified as a native metabolite in the tea plant, and its biosynthesis was shown to be induced by low temperature treatment. Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78 A15 was identified in tea, and its encoded protein was shown to catalyze the glucosylation of eugenol. Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78 A15. Sugar donor preference analysis showed that CsUGT78 A15 had a higher selectivity for glucose, followed by galactose and glucuronic acid. The expression of CsUGT78 A15 was correlated with the accumulation of eugenol glucoside in different tissues and genotypes of tea. Down-regulation of CsUGT78 A15 led to a decreased eugenol glucoside content under cold stress, indicating that CsUGT78 A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress. The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside.
语种:
英文
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茶叶的调节免疫作用
作者:
蔡淑娴;万娟;刘仲华
期刊:
中国茶叶 ,2020年42(4):1-12 ISSN:1000-3150
作者机构:
[万娟; 刘仲华; 蔡淑娴] 国家植物功能成分利用工程技术研究中心
关键词:
茶叶;功能成分;调节免疫;免疫力
摘要:
免疫力是人体抵抗致病微生物,清除自身病变细胞、衰老细胞和损伤细胞等能力.免疫功能是否正常,是人们能否抵抗疾病和保持健康状态的重要基石.免疫学研究证明,人类90%以上的疾病都与免疫系统的功能异常有关.随着人们食物结构和饮食习惯的改变、工作与生活压力的增大、环境污染的加重,以及老龄化社会的来临,导致免疫力变差,使免疫相关的疾病增加.国内外大量研究表明,茶叶及其功能成分(茶多酚/儿茶素、茶氨酸、茶多糖、茶色素等)具有显著的免疫调节作用.茶叶可以通过调节炎症性免疫、代谢性免疫、肠道免疫、癌症免疫以及抑制病原微生物感染,减低人体发生免疫介导性疾病的风险.本文综合国内外的研究成果,从免疫系统与免疫调节、茶叶的免疫调节机制、茶叶活性成分的免疫调节作用和茶叶调节免疫的临床试验效果4个方面阐述了茶叶的免疫调节作用,旨在为健康饮茶增强人体免疫力提供科学依据.
语种:
中文
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Comparing characteristic aroma components of bead-shaped green teas from different regions using headspace solid-phase microextraction and gas chromatography–mass spectrometry/olfactometry combined with chemometrics
作者:
Liu, Panpan;Zheng, Pengcheng;Gong, Ziming* ;Feng, Lin;Gao, Shiwei;...
期刊:
European Food Research and Technology ,2020年246(9):1703-1714 ISSN:1438-2377
通讯作者:
Gong, Ziming;Liu, Zhonghua
作者机构:
[Teng, Jing; Wang, Xueping; Gao, Shiwei; Gong, Ziming; Liu, Panpan; Zheng, Pengcheng; Feng, Lin; Zheng, Lin] Hubei Acad Agr Sci, Inst Fruit & Tea, 10 South Lake Ave, Wuhan, Hubei, Peoples R China.;[Liu, Zhonghua; Liu, Panpan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, 1 Nongda Rd, Changsha, Hunan, Peoples R China.
通讯机构:
[Gong, Ziming; Liu, Zhonghua] H;Hubei Acad Agr Sci, Inst Fruit & Tea, 10 South Lake Ave, Wuhan, Hubei, Peoples R China.;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, 1 Nongda Rd, Changsha, Hunan, Peoples R China.
关键词:
Bead-shaped green tea;Chemometrics;Gas chromatography–mass spectrometry/olfactometry;Key odorants;Volatile constituents
摘要:
In this study, the aroma components of bead-shaped green teas were compared through headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry/olfactometry (GC–MS/O) coupled with chemometrics. A total of 62 volatile compounds were identified in 16 green tea samples. Notably, comparison of GC–MS/O and odour activity values (OAV) results revealed that nonanal, decanal, (E)-2-nonenal, β-ionone, and 1-octen-3-one were the most powerful aroma-active compounds that contributed to the aroma profile of the bead-shaped green teas. Furthermore, multivariate statistical techniques (such as principal component analysis, partial least squares-discriminant analysis, and hierarchical cluster analysis) were used to characterise the bead-shaped green teas according to their geographical origin and the flavour characteristics of their volatile compounds. Additionally, the variable importance in the projection method was used to identify 20 volatile markers that could successfully distinguish bead-shaped green teas on the basis of three flavour characteristics. The results of the analysis revealed that HS-SPME and GC–MS/O coupled with chemometrics can provide an effective method for characterising and classifying bead-shaped green teas. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
语种:
英文
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Multi-omics research in albino tea plants: Past, present, and future
作者:
Zhang, Chenyu;Wang, Minghan;Gao, Xizhi;Zhou, Fang;Shen, Chengwen* ;...
期刊:
Scientia Horticulturae ,2020年261:108943- ISSN:0304-4238
通讯作者:
Shen, Chengwen;Liu, Zhonghua
作者机构:
[Shen, Chengwen; Gao, Xizhi; Wang, Minghan; Liu, Zhonghua; Zhang, Chenyu; Zhou, Fang] Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha, Hunan, Peoples R China.;[Shen, Chengwen; Gao, Xizhi; Wang, Minghan; Liu, Zhonghua; Zhang, Chenyu; Zhou, Fang] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha, Hunan, Peoples R China.;[Shen, Chengwen; Liu, Zhonghua] Hunan Agr Univ, Changsha, Hunan, Peoples R China.
通讯机构:
[Shen, CW; Liu, ZH] H;Hunan Agr Univ, Changsha, Hunan, Peoples R China.
关键词:
Albino tea plants;Camellia sinensis;Omics technology;Catechins;L-theanine
摘要:
The distinct historical background and tea flavor, including high umami taste and low astringency, make the albino tea plants (Atps) stick out from the tea plant (Camellia sinesnis). The decipherment of genes, enzymes, and metabolites involved in these processes is still significantly lagging, which lays an obstacle for molecular plant breeding and commercialized production. With the popularity of high-throughput omics technology, 'single level' or 'multi-level'-based research theory have emerged as powerful tools for revealing the mechanism of characteristic metabolite accumulation and pigment transformation. Thus, it is pivotal to summarize and introduce these advances in facilitating gene identification of critical pathways in the Atps. In this review, we systematically arranged the history and classification of Atps, detailed their strategies for stress response and the metabolic pattern of L-theanine and catechins. Further, reference the successful examples of model plants, we concentrating on the recent advances in omics technology applied to the Atps. Finally, we prospect the promising development areas of the Atps.
语种:
英文
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Catechins enhance skeletal muscle performance
作者:
Li, Penghui;Liu, Ailing;Xiong, Wei;Lin, Haiyan;Xiao, Wenjun;...
期刊:
Critical Reviews in Food Science and Nutrition ,2020年60(3):515-528 ISSN:1040-8398
通讯作者:
Zhang, Sheng
作者机构:
[Liu, Zhonghua; Lin, Haiyan; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Lin, Haiyan; Xiao, Wenjun; Zhang, Sheng; Li, Penghui; Huang, Jianan] Hunan Agr Univ, Collaborat Innovat Ctr Utilisat Funct Ingredients, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha, Hunan, Peoples R China.;[Liu, Ailing] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Chinese Minist Hlth, Key Lab Carcinogenesis, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Chinese Minist Hlth, Canc Res Inst, Key Lab Carcinogenesis & Canc Invas, Changsha, Hunan, Peoples R China.
通讯机构:
[Zhang, Sheng] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.
关键词:
Catechin;metabolism;mitochondrial;muscle atrophy;skeletal muscle
摘要:
Muscle-related disorders, such as sarcopenia and cachexia, caused by aging and chronic diseases can lead to the loss of muscle mass and strength to different degrees, severely affecting human health. Globally, tea is one of the three most popular beverages, and its major active ingredient catechins have been reported to delay muscular atrophy and enhance movement. However, currently, there is no systematic review to elaborate its roles and the associated mechanisms. This article reviews the (1) functions and mechanisms of catechins in the differentiation of myogenic stem cells, biogenesis of mitochondria, synthesis and degradation of proteins, regulation of glucose level, and metabolism of lipids in muscle cells; and (2) effect of catechins on the blood vessels, bones, and nerves that are closely related to the skeletal muscles. Catechins could prevent, mitigate, delay, and even treat muscle-related disorders caused by aging and diseases. © 2019, © 2019 Taylor & Francis Group, LLC.
语种:
英文
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Preparation of theasinensin A and theasinensin B and exploration of their inhibitory mechanism on α-glucosidase
作者:
Tao, Sainan;Chen, Guijie;Xu, Weiqi;Peng, Yujia;Wan, Peng;...
期刊:
Food & Function ,2020年11(4):3527-3538 ISSN:2042-6496
通讯作者:
Zeng, Xiaoxiong;Liu, Zhonghua
作者机构:
[Chen, Guijie; Xu, Weiqi; Tao, Sainan; Peng, Yujia; Wan, Peng; Zeng, Xiaoxiong; Sun, Yi] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.
通讯机构:
[Zeng, Xiaoxiong; Liu, Zhonghua] N;[Liu, Zhonghua] H;Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.
摘要:
Theasinensin A (TSA) and theasinensin B (TSB), dimers of tea catechins produced during the processing of oolong tea and black tea, had superior inhibitory effects on α-glucosidase. However, the potential inhibitory mechanisms on α-glucosidase are still unclear. In the present study, TSA and TSB were chemically synthesized and purified, and their inhibitory effects on α-glucosidase and potential mechanisms were investigated. The results showed that TSA and TSB could inhibit the activity of α-glucosidase in a reversible and noncompetitive manner with IC50 values of 6.342 and 24.464 μg mL-1, respectively, which were much lower than that of acarbose. The fluorescence and circular dichroism spectra revealed that TSA and TSB could alter the microenvironment and the secondary structure of α-glucosidase, thereby decreasing the α-glucosidase activity. Molecular docking results indicated that both TSA and TSB had a strong binding affinity to α-glucosidase by hydrophobic interactions and hydrogen bonds. Moreover, the stronger inhibition of TSA on α-glucosidase might be related to the closer binding site to the active site pocket of α-glucosidase. © The Royal Society of Chemistry.
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英文
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Modulation of gut homeostasis by exopolysaccharides from: Aspergillus cristatus (MK346334), a strain of fungus isolated from Fuzhuan brick tea, contributes to immunomodulatory activity in cyclophosphamide-treated mice
作者:
Xie, Zhiyong;Bai, Yixuan;Chen, Guijie;Rui, Ying;Chen, Dan;...
期刊:
Food & Function ,2020年11(12):10397-10412 ISSN:2042-6496
通讯作者:
Zeng, Xiaoxiong;Liu, Zhonghua
作者机构:
[Chen, Guijie; Rui, Ying; Chen, Dan; Xie, Zhiyong; Zeng, Xiaoxiong; Bai, Yixuan; Sun, Yi] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.
通讯机构:
[Zeng, Xiaoxiong; Liu, Zhonghua] N;[Liu, Zhonghua] H;Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Peoples R China.
摘要:
In this study, the crude exopolysaccharides (CEPSs) from fungus Aspergillus cristatus (MK346334, NCBI) isolated from Fuzhuan brick tea and its main purified fraction (EPSs-2) were investigated. Using the RAW264.7 cell model, EPSs-2 exhibited an excellent immunomodulatory effect in vitro. Then, the regulating effects of EPSs on immune function and gut microbiota were evaluated using a cyclophosphamide (Cy)-induced mice model. It was found that both CEPSs and EPSs-2 improved the body weight loss, immune organ indexes as well as the levels of TNF-α, IL-1β, IFN-γ and IgA, exhibiting potent immunoregulatory activity. Moreover, CEPSs and EPSs-2 not only attenuated the intestinal tissue damage, but also promoted the production of short-chain fatty acids and modulated the microbial composition by increasing the growth of Muribaculaceae, Prevotellaceae_UCG-001, Bacteroides, Parabacteroides and Tidjanibacter, while decreasing the relative abundances of Helicobacter, Bilophila, Mucispirillum, Lachnospiraceae, Ruminococcaceae and Clostridiales. These results indicated that the EPSs, especially EPSs-2, exhibited immunomodulatory activity associated with the modulation of gut microbiota to maintain gut homeostasis, which provided evidence for the development of novel potential prebiotics and immunomodulators. © The Royal Society of Chemistry.
语种:
英文
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The Quality Control of Tea by Near-Infrared Reflectance (NIR) Spectroscopy and Chemometrics
作者:
Zhu, Ming-Zhi;Wen, Beibei;Wu, Hao;Li, Juan;Lin, Haiyan;...
期刊:
Journal of Spectroscopy ,2019年2019 ISSN:2314-4920
通讯作者:
Huang, Jianan;Liu, Zhonghua
作者机构:
[Wen, Beibei; Zhu, Ming-Zhi; Li, Juan; Huang, Jianan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Wen, Beibei; Zhu, Ming-Zhi; Li, Yinhua; Huang, Jianan; Liu, Zhonghua; Lin, Haiyan] Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Wen, Beibei; Zhu, Ming-Zhi; Huang, Jianan; Liu, Zhonghua; Li, Qin] Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.;[Wen, Beibei; Zhu, Ming-Zhi; Liu, Zhonghua] Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.;[Wu, Hao] Xinyang Normal Univ, Coll Life Sci, Key Lab Tea Plant Biol Henan Prov, Xinyang 464000, Peoples R China.
通讯机构:
[Huang, Jianan; Huang, JA; Liu, ZH; Liu, Zhonghua] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Collaborat Innovat Ctr Utilizat Funct Ingredients, Changsha 410128, Hunan, Peoples R China.
摘要:
Tea is known to be one of the most popular beverages enjoyed by two-thirds of the world's population. Concern of variability in tea quality is increasing among consumers. It is of great significance to control quality for commercialized tea products. As a rapid, noninvasive, and nondestructive instrumental technique with simplicity in sample preparation, near-infrared reflectance (NIR) spectroscopy has been proved to be one of the most advanced and efficient tools for the control quality of tea products in recent years. In this article, we review the most recent advances and applications of NIR spectroscopy and chemometrics for the quality control of tea, including the measurement of chemical compositions, the evaluation of sensory attributes, the identification of categories and varieties, and the discrimination of geographical origins. Besides, challenges and future trends of tea quality control by NIR spectroscopy are also presented. © 2019 Ming-Zhi Zhu et al.
语种:
英文
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白叶1号白化过程中叶绿体蛋白质组差异分析
作者:
李勤;程晓梅;李永迪;杨培迪;黄建安;...
期刊:
茶叶科学 ,2019年39(3):325-334 ISSN:1000-369X
作者机构:
湖南农业大学/茶学教育部重点实验室;国家植物功能成分利用工程技术研究中心;湖南省植物功能成分利用协同创新中心;[杨培迪] 湖南省农业科学院茶叶研究所;[李勤; 刘仲华; 李永迪; 黄建安; 程晓梅] 湖南农业大学
关键词:
白叶1号;阶段性白化;叶绿体蛋白质组;双向电泳
摘要:
白叶1号是一种温度敏感型白化茶树品种,叶绿体的变化是其产生阶段性白化现象的关键因素。本研究以白叶1号鲜叶叶绿体为研究对象,采用双向电泳、质谱鉴定结合生物信息学分析,研究阶段性白化过程中叶绿体蛋白的表达差异,探讨白叶1号阶段性白化现象的分子机制。结果表明,在白叶1号白化前期、白化期和复绿期叶绿体中分别识别726、748、718个蛋白质,其中差异表达的蛋白59个,质谱成功鉴定22个差异表达蛋白。生物信息学分析表明,差异表达蛋白直接或间接参与了光合作用、应激响应、核酸代谢、物质代谢和未知功能等,其中与光合作用相关的差异表达蛋白最多,占31.82%,表明阶段性白化现象可能与这些生理功能相关。通过荧光定量PCR分析发现,差异蛋白的基因表达与蛋白表达存在一定差异,这可能是由于蛋白质翻译后加工及修饰造成的。上述研究为进一步揭示白叶1号阶段性白化现象产生的分子机制奠定了理论基础。
语种:
中文
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茶叶的抗衰老作用
作者:
龚雨顺;戴申;黄建安;熊立瑰;刘仲华
期刊:
中国茶叶 ,2019年41(8):6-11 ISSN:1000-3150
作者机构:
国家植物功能成分利用工程技术研究中心 湖南农业大学茶学教育部重点实验室,410128;[戴申; 龚雨顺; 刘仲华; 黄建安; 熊立瑰] 国家植物功能成分利用工程技术研究中心
关键词:
茶;抗衰老作用;老年疾病
摘要:
随着全球老龄化的加剧和生命科学的发展,茶叶抗衰老研究取得了一系列新的进展.在秀丽线虫、果蝇与老鼠等多种动物模型中,茶叶及茶叶主要活性成分茶多酚(儿茶素类、黄酮类以及酚酸类化合物)、咖啡碱与茶氨酸均表现出一定的抗衰老活性.国内外流行病学调研也发现,日常饮茶可以延缓人类衰老,预防或者延缓心血管疾病、Ⅱ型糖尿病与神经退行性疾病等老年疾病的发生.良好的饮茶习惯是促进人类健康的生活方式之一,茶叶抗衰老活性与个体遗传因素、摄入量以及性别相关.
语种:
中文
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Analysis of Young Shoots of "Anji Baicha' (Camellia sinensis) at Three Developmental Stages Using Nontargeted LC-MS-Based Metabolomics
作者:
Zeng, Chaozhen;Lin, Haiyan;Liu, Zhixiang;Liu, Zhonghua*
期刊:
Journal of Food Science ,2019年84(7):1746-1757 ISSN:0022-1147
通讯作者:
Liu, Zhonghua
作者机构:
[Zeng, Chaozhen; Liu, Zhixiang] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Zeng, Chaozhen; Liu, Zhixiang] Hunan Prov Base Sci & Technol Innovat Cooperat, Changsha 410004, Hunan, Peoples R China.;[Zeng, Chaozhen; Liu, Zhonghua] Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;[Zeng, Chaozhen; Liu, Zhonghua; Lin, Haiyan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Zhonghua] H;[Liu, Zhonghua] N;Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.
关键词:
LC-MS;chemical components;developmental stages;metabolomics;'Anji Baicha' (Camellia sinensis)
摘要:
Abstract: ‘Anji Baicha’ (Camellia sinensis) is a low-temperature-sensitive tea variety. During the development of young shoots, the leaves of ‘Anji Baicha’ exhibit periodic albinism. The quality of ‘Anji Baicha’ is closely related to the color of the fresh leaves, with whiter leaves affording a higher amino acid content and superior sensory quality after processing. However, the metabolic mechanism of its quality formation is still unclear. In this study, we analyzed the metabolomic changes of young shoots of ‘Anji Baicha’ and screened for metabolic markers that may be involved in the periodic albinism. Positive- and negative-mode UPLC-QTOF-MS was applied to the metabolomic analysis of young leaves of ‘Anji Baicha’ during three developmental stages (i.e., the pre-albescent, albescent, and regreening stages). The results revealed significant differences in the metabolic profiles of the young leaves at the three stages. The differential metabolites were mainly related to the pathways of flavonoid, phenylpropanoid, and amino acid biosynthesis. The concentrations of several amino acids (primarily l-theanine, l-glutamate, N2-acetyl-l-ornithine, l-aspartic acid, d-proline, l-glutamine, l-leucine, and pyroglutamic acid) and 12-OPDA were significantly higher in the albescent stage. In contrast, during the albescent stages, the concentrations of several carbohydrates (d-fructose, β-d-galactopyranose, 3-O-fucopyranosyl-2-acetamido-2-deoxyglucopyranose, galactose-β-1, 4-xylose acetyl-maltose, and 2-fucosyllactose) were significantly lower. Moreover, catechins (mainly epigallocatechin and catechin derivatives), dimeric catechins (primarily proanthocyanidins), and flavonol and flavonol/flavone glycosides (mainly kaempferol, myricetin, quercetin, cyanidin, and delphinidin glycosides) were detected at the highest levels in the regreening or pre-albescent stages. The obtained results enhance the current understanding of the metabolic mechanisms of periodic albinism and quality development formation in ‘Anji Baicha’. Practical Application: The obtained results not only provide information regarding differential metabolites but also advance the understanding of the mechanism of periodic albinism in ‘Anji Baicha’ at the metabolite level and open up new possibilities for the genetic improvement of tea cultivars. © 2019 Institute of Food Technologists®
语种:
英文
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紫甘薯α-淀粉酶酶解液中紫色素的分离与糖液发酵生产乙醇的研究
作者:
张杨波;兰时乐;董新荣;黄建安;刘仲华
期刊:
食品工业科技 ,2019年40(9):184-188 ISSN:1002-0306
作者机构:
湖南农业大学园艺园林学院,湖南农业大学茶学教育部重点实验室,湖南长沙410128;湖南农业大学生物科学技术学院,湖南长沙,410128;湖南农业大学理学院,湖南长沙,410128;湖南农业大学园艺园林学院,国家植物功能成分利用工程技术研究中心,湖南长沙410128
关键词:
紫甘薯;α-淀粉酶;紫色素;发酵;乙醇
摘要:
本文综合利用紫甘薯资源,同时获得紫色素和糖液,糖液发酵制备乙醇。以大孔树脂分离紫甘薯α-淀粉酶酶解液后获得紫色素与糖液,考察氮源种类与添加量、酵母接种量、发酵时间对糖液发酵产乙醇含量的影响,通过正交实验优化糖液发酵生产乙醇工艺。实验结果表明,紫甘薯酶解液经HPD400型大孔树脂分离,紫色素的色价(E1 cm1%)达到86.3(波长为530 nm),糖液中总糖含量达到55.5 mg/L;(NH4)2SO4作为发酵氮源,(NH4)2SO4添加量为3.0 g/100 mL、酵母接种量为1.5 mL、发酵时间为7 d,可得到13.0%vol乙醇。此方法获得了紫甘薯色素,副产物糖液发酵可生产乙醇,为紫甘薯的综合利用提供了思路。
语种:
中文
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基于教学科研互动的茶学创新人才培养模式探讨
作者:
李银花;李娟;刘仲华;李适;肖文军
期刊:
茶叶通讯 ,2019年46(2):253-256 ISSN:1009-525X
作者机构:
湖南农业大学 园艺园林学院 茶学系,茶学教育部重点实验室,湖南 长沙 410128;[李银花; 刘仲华; 李娟; 李适; 肖文军] 湖南农业大学
关键词:
教学;科研;互动;创新人才;培养模式
摘要:
本科教育是现代高等教育的重要基础。茶学是中国的特色专业,随着茶产业的快速发展,社会迫切需要大量茶学创新型技术人才。本文以湖南农业大学茶学本科专业为例,分析了茶学专业创新人才培养的现状和存在的问题,探索基于教学科研互动的茶学创新人才培养模式,旨在提高茶学专业人才培养质量,为进一步深化茶学专业创新教育改革提供依据。
语种:
中文
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蛋白质羰基化及茶多酚的预防作用研究进展
作者:
左高隆;黄霞;林勇;林海燕;陈美艳;...
期刊:
茶叶通讯 ,2019年46(3):257-262 ISSN:1009-525X
作者机构:
湖南农业大学 茶学教育部重点实验室,国家植物功能成分利用工程技术研究中心,湖南 长沙 410128;湖南农业大学 资源环境学院,湖南 长沙,410128;[林勇; 刘仲华; 黄霞; 左高隆; 陈美艳; 林海燕] 湖南农业大学
关键词:
茶多酚;蛋白质羰基化;氧化应激;进展
摘要:
羰基化蛋白质是机体发生氧化损伤的副产物,其介导了多种疾病的发生与发展,茶多酚作为一种优异的抗氧化剂可以很好的抑制羰基化蛋白质的产生。本文综述了羰基化蛋白质形成的原因及其与疾病发病之间的相关性以及茶多酚的抗氧化能力与其对蛋白质羰基化过程的抑制作用的机制,为防治蛋白质羰基化相关疾病及茶叶高质化利用提供参考。
语种:
中文
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茶叶的抗过敏功效与机理
作者:
林勇;黄建安;王坤波;陈美艳;刘仲华
期刊:
中国茶叶 ,2019年41(3):1-6 ISSN:1000-3150
作者机构:
国家植物功能成分利用工程技术研究中心 湖南农业大学茶学教育部重点实验室,410128;[林勇; 刘仲华; 王坤波; 黄建安; 陈美艳] 国家植物功能成分利用工程技术研究中心
关键词:
过敏反应;茶多酚;儿茶素;甲基化儿茶素;茶黄素;茶皂素
摘要:
从过敏反应的概念、过敏症的群体分布、不同茶类及不同茶叶功能成分的抗过敏功效和抗过敏茶制品开发等方面进行了论述。重点介绍了茶多酚、儿茶素、甲基化儿茶素、茶皂素、茶黄素等茶叶功能成分对过敏反应尤其是速发型过敏具有显著预防与治疗效果,其中茶叶中儿茶素的甲基化产物展现了更为显著的抗过敏功效。一些发酵茶抗过敏能力优于绿茶,这可能与茶叶加工过程中生成除儿茶素外的其他抗过敏活性成分有关,如茶黄素等。茶叶功能成分主要通过以下途径起到抗过敏作用:抑制化学物质诱导的过敏反应;抑制组胺及过敏介质的释放;抑制活性因子如抗体、肾上腺素、酶等引起的过敏反应;促进肾上腺、垂体的活动而有消炎作用。
语种:
中文
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茶叶减肥及对人体代谢综合征的预防功效
作者:
李勤;黄建安;傅冬和;李永迪;刘仲华
期刊:
中国茶叶 ,2019年(5):7-13 ISSN:1000-3150
作者机构:
国家植物功能成分利用工程技术研究中心 湖南农业大学茶学教育部重点实验室,410128;[傅冬和; 李勤; 刘仲华; 李永迪; 黄建安] 国家植物功能成分利用工程技术研究中心
关键词:
茶叶;活性成分;降脂减肥;代谢综合征
摘要:
从肥胖症、高血脂症等代谢综合征的概念、不同茶叶功能成分及不同茶类降脂减肥功效、相关降脂减肥茶制品的开发等方面进行了论述。重点介绍了茶多酚/儿茶素、茶黄素和咖啡碱等茶叶功能成分的降脂减肥功效及其降脂减肥机理,以及不同茶类降脂减肥功效的差异。现有研究结果表明,茶叶主要通过抑制脂肪合成相关酶的表达、促进脂肪酸氧化、抑制食欲、抑制营养物质的吸收等作用达到降脂减肥的功效。
语种:
中文
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Real-time monitoring of optimum timing for harvesting fresh tea leaves based on machine vision
作者:
Zhang, Liang;Zhang, Hongduo;Chen, Yedong;Dai, Sihui;Li, Xumeng* ;...
期刊:
国际农业与生物工程学报(英文) ,2019年12(1):6-9 ISSN:1934-6344
通讯作者:
Li, Ming;Li, Xumeng
作者机构:
[Li, Ming; Zhang, Liang; Chen, Yedong; Zhang, Hongduo] Hunan Agr Univ, Coll Engn, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua; Dai, Sihui] Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China.;[Li, Xumeng] Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.;[Imou, Kenji] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biol & Environm Engn, Tokyo 1130033, Japan.;[Li, Ming] Shandong Univ Technol, Sch Agr Engn & Food Sci, Zibo 255000, Peoples R China.
通讯机构:
[Li, Ming; Li, Xumeng] H;Hunan Agr Univ, Coll Engn, Changsha 410128, Hunan, Peoples R China.;Hunan Agr Univ, Coll Agr, Changsha 410128, Hunan, Peoples R China.
关键词:
Agricultural machinery;Fresh tea leaves;Intelligent recognition;Machine vision;Real-time monitoring
摘要:
The harvesting time of fresh tea leaves has a significant impact on product yield and quality. The aim of this study was to propose a method for real-time monitoring based on machine vision to determine the optimum harvesting time for picking fresh tea leaves. Firstly, the shapes of fresh tea leaves were distinguished from RGB images of the tea-tree canopy after graying with the improved B-G algorithm, filtering with a median filter algorithm, binary processing with the Otsu algorithm, and noise reduction and edge smoothing using open and close operations. Then the leaf characteristics, such as leaf area index, average length, and leaf identification index, were calculated. Based on these, the Bayesian discriminant principle and method were used to construct a discriminant model for fresh tea-leaf collection status. When this method was applied to a RGB tea-tree canopy image acquired at 45°shooting angle, the fresh tea-leaf recognition rate was 90.3%, and the accuracy for fresh tea-leaf harvesting status was 98% by cross validation. Hence, this method provides the basic conditions for future tea-plantation operation and management using information technology, automation, and intelligent systems. © 2018, ABE Publishing.
语种:
英文
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Analysis of bacterial and fungal communities by Illumina MiSeq platforms and characterization of Aspergillus cristatus in Fuzhuan brick tea
作者:
Rui, Ying;Wan, Peng;Chen, Guijie;Xie, Minhao;Sun, Yi;...
期刊:
LWT ,2019年110:168-174 ISSN:0023-6438
通讯作者:
Zeng, Xiaoxiong;Liu, Zhonghua
作者机构:
[Chen, Guijie; Xie, Minhao; Rui, Ying; Wan, Peng; Zeng, Xiaoxiong; Sun, Yi] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;[Liu, Zhonghua] Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhonghua] Natl Res Ctr Engn Technol Utilizat Bot Funct Ingr, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Zeng, Xiaoxiong] N;[Liu, Zhonghua] H;Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China.;Hunan Agr Univ, Minist Educ Tea Sci, Key Lab, Changsha 410128, Hunan, Peoples R China.
关键词:
Fuzhuan brick tea;Illumine MiSeq sequencing;Microbial community;Fungi;Aspergillus
摘要:
Fuzhuan brick tea (FBT) is an indigenous dark tea fermented by fungal and bacterial communities, and the special favor of FBT attributes to the actions of microbiota, while the correlation between bacteria and fungi attracts little attention. In the present study, therefore, the compositions of microbial communities in 10 samples of FBT from four tea processing factories were evaluated by Illumina MiSeq sequencing. Bacterial taxonomy analysis revealed that the phylum Firmicutes and Proteobacteria were the predominant bacteria in all samples. Fungal taxonomy analysis revealed that the phylum Ascomycota, family Trichocomaceae, genus Aspergillus was the dominant fungus in all samples. Moreover, Aspergillus (OTU1, OTU2, OTU16, OTU30, OTU31 and OTU37), Wallemia (OTU3), Penicillium (OTU32), Candida (OTU33, OTU35, OTU40 and OTU43), Debaryomyces (OTU48) and Rhodotorula (OTU68) showed higher correlation with bacteria in FBT. Furthermore, a strain Aspergillus cristatus was isolated from FBT, and its morphology and molecular taxonomy were observed and analyzed by scanning electron microscopy and sequencing. Hence, this is the first report to reveal the correlation between bacterial and fungal communities in FBT, and A. cristatus may have potential application to improve the quality of FBT.
语种:
英文
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Characterization of the transcriptional regulator CsbHLH62 that negatively regulates EGCG3"Me biosynthesis in Camellia sinensis
作者:
Luo, Yong;Yu, Shuang-shuang;Li, Juan;Li, Qin;Wang, Kun-Bo* ;...
期刊:
Gene ,2019年699:8-15 ISSN:0378-1119
通讯作者:
Wang, Kun-Bo;Huang, Jian-an;Liu, Zhong-hua
作者机构:
[Huang, Jian-an; Yu, Shuang-shuang; Liu, Zhong-hua; Wang, Kun-Bo; Wang, KB; Huang, JA; Li, Qin; Luo, Yong; Li, Juan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.;[Huang, Jian-an; Liu, Zhong-hua; Wang, Kun-Bo; Li, Qin; Li, Juan] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.;[Huang, Jian-an; Liu, Zhong-hua; Wang, Kun-Bo; Li, Qin; Li, Juan] Hunan Agr Univ, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wang, KB; Huang, JA; Liu, ZH] H;Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.
关键词:
CCoAOMT;Camellia sinensis;CsDFR;CsLAR;EGCG3"Me biosynthesis;bHLH
摘要:
Epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3”Me) in tea (Camellia sinensis (L.) O. Kuntze) is a major source of O-methylated catechin and renowned for a wide range of health effects. However, the transcriptional regulation mechanisms of EGCG3”Me biosynthesis remain unclear. In the present work, the basic Helix-Loop-Helix (bHLH) transcription factor, designated as CsbHLH62, belonging to GBOF group of bHLH families, was isolated and characterized from Camellia sinensis. CsbHLH62 contains an Open Reading Frame of 1662 bp and encodes a polypeptide of 553 amino acids. Subcellular location and transcriptional activity analysis showed it as a nucleus protein and possessed transcriptional inhibition activity. Furthermore, the expression of CsbHLH62 was decreased during EGCG3”Me accumulation. More importantly, E-box motifs (5′-CANNTG-3′) were found in the promoters of CCoAOMT, CsLAR, and CsDFR, and further transient expression assays showed that CsbHLH62 repressed the transcription of CCoAOMT, CsLAR, and CsDFR. Collectively, these results suggest that CsbHLH62 acts as a transcriptional repressor that might be negatively affecting the accumulation of EGCG3”Me. These findings provide novel insights into the regulatory mechanism of EGCG3”Me biosynthesis, which might help to breed high EGCG3”Me-content tea plants. © 2019 Elsevier B.V.
语种:
英文
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