作者机构：
[刘川; 黄嘉欣; 夏菠; 易有金; 罗程印] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[李高阳] Hunan Agricultural Product Processing Institute, Changsha, 410128, China;[柏连阳] Hunan Academy of Agricultural Sciences, Changsha, 410128, China
摘要：
Benzo[a]pyrene (B[a]P) exposure has been associated with the alteration in epigenetic marks that are involved in cancer development. Biotinidase (BTD) and holocarboxylase synthetase (HCS) are 2 major enzymes involved in maintaining the homeostasis of biotinylation, and the deregulation of this pathway has been associated with a number of cancers. However, the link between B[a]P exposure and the dysregulation of BTD/HCS in B[a]P-associated tumorigenesis is unknown. Here we showed that the expression of both BTD and HCS was significantly decreased upon B[a]P treatment in human bronchial epithelial (16HBE) cells. Benzo[a]pyrene exposure led to the global loss of DNA methylation by immunofluorescence, which coincided with the reduction in acetylation levels on histones H3 and H4 in 16HBE cells. Consistent with decreased histone acetylation, histone deacetylases (HDACs) HDAC2 and HDAC3 were significantly upregulated in a dosage-dependent manner. When DNA methylation or HDAC activity was inhibited, we found that the reduction in BTD and HCS was separately regulated through distinct epigenetic mechanisms. Together, our results suggested the potential link between B[a]P toxicity and deregulation of biotin homeostasis pathway in B[a]P-associated cancer development.
通讯机构：
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词：
内生细菌;发酵;菌种鉴定;保鲜;辣椒
摘要：
从辣椒果实中分离、筛选出了一株对辣椒炭疽病菌具有拮抗作用的内生细菌P5,通过菌体、菌落形态观察、生理生化测定及16S r DNA序列分析,鉴定P5为枯草芽孢杆菌。以辣椒炭疽病作为指示菌,测定其发酵滤液理化性质。用发酵滤液处理辣椒进行采后保鲜研究,测定品质指标:质量损失率、腐烂率、可滴定酸含量、Vc含量、总糖含量、叶绿素含量等品质指标。结果表明,发酵滤液热稳定性良好,在p H 8.0以下,40100℃范围内,抑菌活性稳定无变化,121℃处理30 min活性有所下降,为对照组的85%;对p H稳定,在室温下,p H 2.012范围内,抑菌活性不发生变化;对紫外线稳定,经紫外光照射12 h,其活性仍为对照的89.87%。发酵滤液处理过的辣椒保鲜效果明显优于无菌水对照组,显著降低了辣椒失重率和腐烂率,并有效抑制了果实中可滴定酸、Vc、总糖、叶绿素等含量的减少,对辣椒有显著的保鲜效果。
作者机构：
[程远渡; 易有金; 夏菠; 周红丽; 曹熙; 罗程印] Hunan Provincial Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China
摘要：
目的:探讨植物甾醇酯和葛根素联合使用对肥胖小鼠的减肥作用。方法:饲喂高脂饲料建立小鼠肥胖模型,将肥胖小鼠按体质量分为5组(肥胖模型组、植物甾醇酯组、葛根素组、植物甾醇酯和葛根素联合组、奥利司他组),每组10只,连续灌胃6周。观察药物干预后小鼠体质量、摄食量、肥胖指数(Lee’s指数)、脂肪系数,血清总胆固醇(total cholestetol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)水平,脂肪细胞数量和大小、肝脏病变的情况。结果:与肥胖模型组相比,植物甾醇酯组、葛根素组、植物甾醇酯和葛根素联合组小鼠体质量分别降低5.41%、6.98%、12.50%;Lee’s指数分别降低3.41%、3.26%、6.25%;脂肪系数分别降低19.88%、27.41%、30.42%。3组血清TC、TG、LDL-C水平均低于肥胖模型组,其中植物甾醇酯和葛根素联合组降幅最大,分别为21.40%、37.50%、30.43%。植物甾醇酯和葛根素联合组小鼠的脂肪细胞直径变小、数量增多、肝脏脂肪性病变得到缓解。结论:植物甾醇酯和葛根素都能抑制小鼠肥胖,其中两者联合使用时减肥效果最好。
作者机构：
[周金伟; 夏菠; 周红丽; 隆丽林; 易有金; 程远渡] Hunan Provincial Key Laboratory of Food Science and Biotechnology/College of Food Science and Technology, Hunan Agricultural University, Changsha, China;[李高阳] Institute of Agro-product Processing Science and Technology, Hunan Provincial Academy of Agricultural Science, Changsha, China;[柏连阳] Hunan Agriculture Academy of Science, Changsha, China
通讯机构：
Hunan Provincial Key Laboratory of Food Science and Biotechnology/College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词：
Apoptosis;Death receptor pathway;Ganoderma lucidum polysaccharides;Human colon cancer
摘要：
Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE-cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP-mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases-3, -8 and -9 was involved in GLP-stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase-3 proteins, whilst reducing the expression of cleaved poly(ADP-ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.
