Acetylome profiling reveals extensive involvement of lysine acetylation in the conversion of muscle to meat
作者：
Jiang, Shengwang;Liu, Yisong;Shen, Zhenglei;Zhou, Bing;Shen, Qingwu W.*
期刊：
Journal of Proteomics ,2019年205:103412 ISSN：1874-3919
通讯作者：
Shen, Qingwu W.
作者机构：
[Jiang, Shengwang; Shen, Qingwu W.; Liu, Yisong; Zhou, Bing] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shen, Zhenglei] Nanya Middle Sch, Changsha 410129, Hunan, Peoples R China.
通讯机构：
[Shen, Qingwu W.] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Acetylome;Meat quality;Postmortem;Proteomics
摘要：
Protein lysine acetylation is an post-translational modification that regulates gene expression, metabolism, cell signaling, and diseases, but its implication in the postmortem (PM) meat quality development is basically unclear. In the present study, a quantitative proteomic analysis was conducted to profile acetylome in porcine muscle within 24 h PM. In total 595 acetylation sites assigned to 163 proteins were identified in porcine muscle, of which 460 sites distributing to 110 proteins significantly changed in acetylation levels in the conversion of muscle to meat. The dynamic acetylation/deacetylaion of muscle proteins was closely associated with critical chemical-biophysical changes in PM muscle. Bioinformatic analysis revealed that protein lysine acetylation likely regulated postmortem meat quality development by regulating glycolysis and muscle pH, cell stress reponse and apoptosis, muscle contraction and rigor mortis, calcium signaling and proteolysis, IMP synthesis and meat flavor development, and even the stability of pigment proteins and meat color. This study provided the first overview of protein lysine acetylation in PM muscle and revealed its significance in the conversion of muscle to meat. Future exploration of the exact role of protein lysine acetylation at specific sites will further our understanding regarding the underlying mechanisms and be helpful for meat quality control. Significance: This is the first analysis of acetylome in farm animal and postmortem muscle. Our data showed that the dynamic acetylation/deacetylation of muscle proteins was closely related to the postmortem changes of muscle that affect the final quality of raw meat. Proteins related to glucose metabolism and muscle contraction were the two largest clusters of acetylproteins identified in postmortem porcine muscle. Networks of acetylproteins involved in apoptosis, calcium signaling and IMP synthesis were identified in postmortem porcine muscle at the same time. Our results revealed that protein lysine acetylation regulated the conversion of muscle to meat. It likely regulated meat quality development by regulating postmortem glycolysis, mitochondrion initiated cell apoptosis, calcium signaling, rigor mortis, meat flavor compound sysnthesis and meat tenderization. Our study broadened our understanding of the biochemistry regulating the postmortem conversion of muscle to meat and final meat quality development, which may be helpful for future meat quality control. © 2019
语种：
英文
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Non-muscle Myosin II: Role in microbial infection and its potential as a therapeutic target
作者：
Tan, Lei;Yuan, Xiaomin;Liu, Yisong;Cai, Xiong;Guo, Shiyin* ;...
期刊：
Frontiers in Microbiology ,2019年10(MAR):401 ISSN：1664-302X
通讯作者：
Wang, Aibing;Guo, Shiyin
作者机构：
[Liu, Yisong; Wang, Aibing; Tan, Lei; Yuan, Xiaomin] Hunan Agr Univ, Coll Vet Med, Res & Dev Ctr Anim Reverse Vaccinol Hunan Prov, Hunan Prov Key Lab Prot Engn Anim Vaccines, Changsha, Hunan, Peoples R China.;[Cai, Xiong] Hunan Univ Chinese Med, Inst Innovat & Appl Res Chinese Med, Changsha, Hunan, Peoples R China.;[Guo, Shiyin] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Wang, Aibing; Guo, Shiyin] H;Hunan Agr Univ, Coll Vet Med, Res & Dev Ctr Anim Reverse Vaccinol Hunan Prov, Hunan Prov Key Lab Prot Engn Anim Vaccines, Changsha, Hunan, Peoples R China.;Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.
关键词：
co-factors/receptors;mammalian cells;microbial-triggered discords;non-muscle myosin II;regulators;regulatory pathways
摘要：
Currently, the major measures of preventing and controlling microbial infection are vaccinations and drugs. However, the appearance of drug resistance microbial mounts is main obstacle in current anti-microbial therapy. One of the most ubiquitous actin-binding proteins, non-muscle myosin II (NM II) plays a crucial role in a wide range of cellular physiological activities in mammals, including cell adhesion, migration, and division. Nowadays, growing evidence indicates that aberrant expression or activity of NM II can be detected in many diseases caused by microbes, including viruses and bacteria. Furthermore, an important role for NM II in the infection of some microbes is verified. Importantly, modulating the expression of NM II with small hairpin RNA (shRNA) or the activity of it by inhibitors can affect microbial-triggered phenotypes. Therefore, NM II holds the promise to be a potential target for inhibiting the infection of microbes and even treating microbial-triggered discords. In spite of these, a comprehensive view on the functions of NM II in microbial infection and the regulators which have an impact on the roles of NM II in this context, is still lacking. In this review, we summarize our current knowledge on the roles of NM II in microbial-triggered discords and provide broad insights into its regulators. In addition, the existing challenge of investigating the multiple roles of NM II in microbial infection and developing NM II inhibitors for treating these microbial-triggered discords, are also discussed. Copyright © 2019 Tan, Yuan, Liu, Cai, Guo and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
语种：
英文
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Detection of Edible Plant Oil Adulteration by Triacylglycerol Profiles Using an Atmospheric Pressure Chemical Ionization Source and MS3 Ion Trap Mass Spectrometry
作者：
Wang, Xiupin;Li, Peiwu* ;Liu, Xia;Liu, Youqian;Zhang, Qi;...
期刊：
European Journal of Lipid Science and Technology ,2019年121(12):1900029-1-1900029-8 ISSN：1438-7697
通讯作者：
Li, Peiwu
作者机构：
[Wang, Xiupin; Li, Peiwu; Liu, Youqian; Zhang, Qi; Zhang, Liangxiao] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China;[Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Zhang, Qi; Zhang, Liangxiao] Natl Reference Lab Agr Testing Mycotoxin, Wuhan 430062, Hubei, Peoples R China;[Wang, Xiupin; Li, Peiwu; Liu, Youqian; Zhang, Qi; Zhang, Liangxiao] Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Hubei, Peoples R China
通讯机构：
[Li, Peiwu] C;[Li, Peiwu] M;[Li, Peiwu] N;Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Hubei, Peoples R China. Natl Reference Lab Agr Testing Mycotoxin, Wuhan 430062, Hubei, Peoples R China. Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Hubei, Peoples R China.
关键词：
adulterated edible plant oil;multivariate statistical methods;RPLC-APCI-IT-MS;triacylglycerol profiles
摘要：
An adulteration of high-price oil has been an important concern in agri-food fraud problems. Triacylglycerols are the main components of edible plant oils, which play an important role as target for adulteration detections. The objective is to evaluate the utility of triacylglycerol profiles in the detection of adulteration in high priced oils. An effective method is established to detect adulteration of high priced oils based on triacylglycerol profiles and chemometrics. Triacylglycerol profiles of edible oils are analyzed by an APCI-MS3-IT-MS. All triacylglycerol compounds are quantified by normalization of the chromatographic peak area in selected reaction monitoring (SRM) mode based on MS3 fragment ion pairs, which are used to eliminate interference from the isobaric species of triacylglycerol. The results of the hierarchical cluster analysis (HCA) and random forest (RF) classification algorithm indicated that peanut, soybean, sesame, sunflower seed, and linseed oils are completely classified into five groups based on their triacylglycerol profiles. Finally, a recursive support vector machine (R-SVM) discriminant model is established, which can successfully identify adulteration of high priced oil with cheaper edible oils at a concentration of as low as 4% with an accuracy of 93.7%. Practical Applications: The triacylglycerol profiles of edible plant oils are analyzed by RPLC-APCI-IT-MS. The SRM scan mode based on MS3 fragment ion pairs can eliminate interference of triacylglycerol isomers. The discriminant model for adulterated high-price oils is established by R-SVM. HCA, and RF can classify the five kinds of edible plant oil. The triacylglycerol of five edible plant oils is analyzed by RPLC-APCI-MS3-IT-MS. The triacylglycerol data matrix is submitted using unsupervised (HCA) and supervised (RF) chemometric methods to classify five kinds of edible plant oil. A discriminant model established with the R-SVM can be used to identify adulterated linseed oil samples (≥4%) with an accuracy of 93.7%. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
语种：
英文
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Proteomic analysis reveals that lysine acetylation mediates the effect of antemortem stress on postmortem meat quality development
作者：
Zhou, Bing;Shen, Zhenglei;Liu, Yisong;Wang, Chengtao* ;Shen, Qingwu W.*
期刊：
Food Chemistry ,2019年293:396-407 ISSN：0308-8146
通讯作者：
Wang, Chengtao;Shen, Qingwu W.
作者机构：
[Shen, Qingwu W.; Zhou, Bing; Wang, Chengtao] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 11 Fucheng Rd, Beijing 100048, Peoples R China.;[Shen, Qingwu W.; Liu, Yisong; Zhou, Bing] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Shen, Zhenglei] Nanya Middle Sch, Changsha 410129, Hunan, Peoples R China.
通讯机构：
[Wang, Chengtao] B;[Shen, Qingwu W.] H;Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 11 Fucheng Rd, Beijing 100048, Peoples R China.;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Antemortem stress;Glycolysis;Meat quality;Protein lysine acetylation;Proteomics
摘要：
To explore the involvement of protein lysine acetylation in the conversion of muscle to meat, a quantitative analysis of the acetylome in postmortem porcine muscle with or without antemortem stress was conducted. In total, 771 acetylpeptides containing 681 lysine acetylation sites mapping to 176 acetylproteins were identified. Acetylproteins were enriched in muscle contraction, carbohydrate metabolism, cell apoptosis and calcium signaling. Bioinformatic analysis suggests that preslaughter handling may be associated with glycolysis in postmortem muscle and the overall meat quality, via acetylation of multiple enzymes of glycogenolysis/glycolysis, regulate rigor mortis via acetylation of contractile, ATP production and calcium signaling-related proteins, and regulate stress response, cell apoptosis and meat tenderization via regulating the functions of heat shock proteins and permeability transition pore complex. This study provides the first overview of the acetylome in postmortem muscle as affected by preslaughter handling and broadens knowledge of the biochemistry regulating meat quality development. © 2019
语种：
英文
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Phosphorylation of nucleolin is indispensable to upregulate miR-21 and inhibit apoptosis in cardiomyocytes
作者：
Tong, Zhongyi;Tang, Yuting;Jiang, Bimei* ;Wu, Yanyang;Liu, Yanjuan;...
期刊：
JOURNAL OF CELLULAR PHYSIOLOGY ,2019年234(4):4044-4053 ISSN：0021-9541
通讯作者：
Jiang, Bimei
作者机构：
[Xiao, Xianzhong; Li, Yuanbin; Tong, Zhongyi; Liu, Yanjuan; Jiang, Bimei; Tang, Yuting] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha, Hunan, Peoples R China.;[Tong, Zhongyi] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha, Hunan, Peoples R China.;[Wu, Yanyang] Hunan Agr Univ, Food Sci & Technol Coll, Dept Microbiol, Changsha, Hunan, Peoples R China.
通讯机构：
[Jiang, Bimei] C;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha, Hunan, Peoples R China.
关键词：
ago2;miR-21;myocardial ischemia reperfusion injury;nucleolin;phosphorylation
摘要：
Nucleolin is a multifunctional phosphoprotein and is involved in protecting from myocardial ischemia/reperfusion (I/R) injury. The function of nucleolin is regulated by posttranslational modifications, including phosphorylation and glycosylation. To study whether phosphorylation of nucleolin (P-nucleolin) was involved in the protection from myocardial I/R injury. We investigated the expression pattern of P-nucleolin (Thr-76 and 84) in hearts subjected to I/R injury, or rat cardiac myoblast cells (H9C2) subjected to hydrogen peroxide (H 2 O 2 ). The results showed that the expression of P-nucleolin and the ratio of P-nucleolin/nucleolin were significantly increased both in vivo and in vitro. Mutant nucleolin was obtained by site directed mutagenesis in vitro: threonine at 76 and 84 was replaced by alanine, and we found that the protective effect of nucleolin on apoptosis induced by oxidative stress was dependent on its phosphorylation at 76 and 84 in H9C2 cells. Furthermore, the cardio-protective roles of P-nucleolin (Thr-76 and 84) in H9C2 cardiomyocytes, were attributable to the upregulation of microRNA (miR)-21. Further analysis found that P-nucleolin (Thr-76 and 84) could bind to miR-21, and P-nucleolin colocalized with argonaute 2 (Ago2) in cytoplasm and could interact with Ago2 in a RNA-independent manner under cell oxidative stress. The current study revealed that P-nucleolin (Thr-76 and 84) increased in I/R injury myocardium, P-nucleolin was indispensable to upregulate miR-21 and inhibited apoptosis induced by H 2 O 2 in H9C2 cardiomyocytes. These findings provided new insight into the molecular mechanisms of nucleolin in myocardial I/R injury and oxidative stress cells.
语种：
英文
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菠菜鸡蛋干加工工艺的优化
作者：
代昕冉;刘焱;赵晨
期刊：
食品工业 ,2018年39(12):64-69 ISSN：1004-471X
作者机构：
湖南农业大学食品科技学院 长沙410128;湖南农业大学,湖南省发酵食品工程技术中心 长沙410128;湖南津之源食品科技股份有限公司 长沙410128
关键词：
鸡蛋干;菠菜汁;加工工艺;配方
摘要：
为开拓蛋品加工新途径,提高蛋品价值,需对蛋制品加工进行创新。鸡蛋干是一种新型深加工蛋品,蔬菜鸡蛋干则是进一步的优化创新,此产品以鸡蛋全蛋液为主原料,添加蔬菜制作而成。采用单因素试验和响应面试验对产品的配方及工艺进行优化,得到最佳配方为:0.4%食盐、0.5%味精、0.5%鸡精、0.848%白砂糖、0.3%卡拉胶、0.6%焦磷酸钠、0.2%三聚磷酸钠、0.4%六偏磷酸钠、20%菠菜汁和0.1%CMC-Na。此条件下,将蛋蔬混合液蒸10min,焖5 min,在110℃下杀菌20 min,所得产品的口感最佳、弹性及硬度最好。
语种：
中文
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液相色谱-串联质谱法同时测定中草药保健食品中15种有机磷和氨基甲酸酯类农药残留
作者：
姜成君;殷帅;孟庆玉;张会亮;刘焱
期刊：
食品安全质量检测学报 ,2017年8(7):2495-2501 ISSN：2095-0381
作者机构：
湖南农业大学食品科学技术学院,长沙410001;湖南省药品检验研究院,长沙410001;湖南省药品检验研究院,长沙,410001;中国食品药品检定研究院,北京,100050;湖南农业大学食品科学技术学院,长沙,410001
关键词：
液相色谱-串联质谱法;中草药保健食品;有机磷;氨基甲酸酯
摘要：
目的 建立高效液相色谱-串联质谱法同时测定中草药保健食品中15种有机磷和氨基甲酸酯类农药残留.方法 样品经乙腈提取后,高速离心,经分散型固相萃取小柱净化.采用Atlantis C18(2.1 mm× 100 mm,3 μm)柱,以0.1％甲酸-乙腈为流动相,梯度洗脱分离.质谱采用正离子模式(electrospray ionization,ESI+),多反应监测模式(multiple reaction monitoring,MRM)进行检测,基质曲线外标法定量.结果 该方法对15种农药残留的线性范围为0.001～0.1 μg/mL,线性相关系数r≥0.993,加标回收率为82.7％～113.3％,相对标准偏差为2.7％～10.6％,检出限为0.8～24.6 μg/kg.结论 本方法操作简单、快捷,适用于中草药保健食品中15种有机磷和氨基甲酸酯类农药残留的同时检测,为保健食品质量控制提供依据.
语种：
中文
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Surface plasmon resonance immunosensor for fast, highly sensitive, and in situ detection of the magnetic nanoparticles-enriched Salmonella enteritidis
作者：
Liu, Xia* ;Hu, Yuxin;Zheng, Shu;Liu, Yan;He, Zao;...
期刊：
Sensors and Actuators B-Chemical ,2016年230:191-198 ISSN：0925-4005
通讯作者：
Liu, Xia
作者机构：
[Liu, Yan; He, Zao; Hu, Yuxin; Luo, Fang; Liu, Xia; Zheng, Shu] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.;[Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
关键词：
Surface plasmon resonance immunosensor;Salmonella enteritidis detection;Immune magnetic nanoparticles separation;Sandwich assay
摘要：
A highly sensitive surface plasmon resonance (SPR) immunosensor combining antibody-functionalized Fe<inf>3</inf>O<inf>4</inf>magnetic nanoparticles (immunoMNPs) separation is reported for rapid detection of Salmonella enteritidis (S. enteritidis) based on the sandwich immunoassay. Here, immunoMNPs simultaneously served not only as "vehicles" for rapid delivery of analyte from a sample to the sensor surface, but also as labels increasing the measured refractive index changes associated with the binding of analyte. S. enteritidis can be detected at the concentration as low as 14 cfu/mL with a good linear signal range at 1.4 ×10<sup>1</sup>-1.4 ×10<sup>9</sup>cfu/mL. Moreover, the use of immunoMNPs gives 4 orders of magnitude improvement in the sensitivity toward S. enteritidis compared with regular SPR immunosensor with direct detection format. The selectivity of proposed SPR approach was examined with Escherichia coli K12 ER2738 and Lactobacillus LJ-3. The recovery of 92.76-113.25% was obtained in eggshell. The developed SPR immunosensor has potential to provide a simple, low-cost and sensitive method for in situ detection of foodborne pathogens. ©2016 Published by Elsevier B.V.
语种：
英文
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Different actions of salt and pyrophosphate on protein extraction from myofibrils reveal the mechanism controlling myosin dissociation
作者：
Shen, Qingwu W.;Swartz, Darl R.;Wang, Zhenyu;Liu, Yue;Gao, Yuan;...
期刊：
Journal of the Science of Food and Agriculture ,2016年96(6):2033-2039 ISSN：0022-5142
通讯作者：
Zhang, Dequan
作者机构：
[Shen, Qingwu W.] Chinese Acad Agr Sci, Key Lab Agroprod Proc, Inst Food Sci & Technol, Minist Agr, Beijing 100193, Peoples R China.;[Shen, Qingwu W.; Gao, Yuan; Zhang, Dequan; Liu, Yue; Wang, Zhenyu] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Swartz, Darl R.] Delaware Valley Coll, Doylestown, PA 18901 USA.;[Zhang, Dequan] Chinese Acad Agr Sci, Inst Food Sci & Technol, 1 NongDa South Rd, Beijing 100193, Peoples R China.
通讯机构：
[Zhang, Dequan] C;Chinese Acad Agr Sci, Inst Food Sci & Technol, 1 NongDa South Rd, Beijing 100193, Peoples R China.
关键词：
myosin;myosin extraction;salt;MgPPi;turbidity assay;meat
摘要：
Background: Myosin is the major functional protein in muscle foods for water retention, protein binding/gelation and fat holding/emulsification. To maximize its functionality, myosin needs to be released from thick filaments. Understanding of the mechanism controlling myosin extraction will help improve quality traits of meat products. Results: The data obtained show that actomyosin binding is the rate-limiting constraint for myosin release in rigor condition. Magnesium pyrophosphate (MgPPi) increased myosin extraction by weakening actomyosin interaction and maximized myosin extraction at 0.4 mol L-1 NaCl, which was not attained at 1.0 mol L-1 NaCl in the absence of PPi. Interaction between myosin rod domains is another critical constraint for myosin extraction, which is, rather than PPi, salt dependent. Further, our data suggest that MyBP-C (myosin binding protein C) and M-line might not be of significance in the process of NaCl-induced myosin extraction, though further study was needed. Conclusion: Our study provides new insight into the mechanism that controls myosin extraction from intact sarcomere, which could be applied to maximize myosin function and to improve meat quality in practice. © 2016 Society of Chemical Industry.
语种：
英文
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Role of the ubiquitin-proteasome pathway on proteolytic activity in postmortem proteolysis and tenderisation of sheep skeletal muscle
作者：
Liu, Yue;Du, Manting;Li, Xin;Chen, Li;Shen, Qingwu;...
期刊：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ,2016年51(11):2353-2359 ISSN：0950-5423
通讯作者：
Zhang, Dequan
作者机构：
[Du, Manting; Zhang, Dequan; Liu, Yue; Chen, Li; Li, Xin] Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, Inst Food Sci & Technol, Beijing 100193, Peoples R China.;[Tian, Jianwen; Liu, Yue] Ningxia Univ, Sch Agr, Ningxia 750021, Peoples R China.;[Shen, Qingwu] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Zhang, Dequan] C;Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Proc, Inst Food Sci & Technol, Beijing 100193, Peoples R China.
关键词：
Proteasome;sheep;skeletal muscle;tenderisation;ubiquitin
摘要：
The objective of this study was to investigate the effects of ubiquitin-proteasome pathway on meat tenderisation. The sheep muscle longissimus lumborum was injected with or without PYR-41 (inhibitor of ubiquitination) or MG-132 (inhibitor of proteasome). Muscle samples were collected at 6, 15, 24 and 48h after injection. Myofibrillar protein degradation, muscle ultrastructure and sarcomere length were determined. Results showed that inhibition of proteasome or ubiquitination affected sarcomere length at 48h after treatments. Destruction of muscle ultrastructure in both treatments was reduced when compared to control. Inhibition of proteasome produced different fragments of myofibrillar proteins in comparison with control at 48h. In conclusion, ubiquitin-proteasome plays a role in postmortem proteolysis and might contribute to meat tenderisation.
语种：
英文
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Electrochemical Sensor based on Imprinted Sol-Gel Polymer on Au NPs-MWCNTs-CS Modified Electrode for the Determination of Acrylamide
作者：
Liu, Xia* ;Mao, Lu-Gang;Wang, Yuan-Liang;Shi, Xing-Bo;Liu, Yan;...
期刊：
Food Analytical Methods ,2016年9(1):114-121 ISSN：1936-9751
通讯作者：
Liu, Xia
作者机构：
[Shi, Xing-Bo; Liu, Yan; Wang, Yuan-Liang; He, Zao; Mao, Lu-Gang; Yang, Yang; Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Liu, Xia] H;Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Changsha 410128, Hunan, Peoples R China.
关键词：
Electrochemical sensor;Acrylamide;Gold nanoparticles;Chitosan;Multiwalled carbon nanotubes;Sol-gel molecularly imprinted
摘要：
A sensitive molecularly imprinted electrochemical sensor was successfully constructed for the detection of acrylamide (AM). It is based on a glassy carbon electrode modified with a composites prepared from gold nanoparticles, multiwalled carbon nanotubes, and chitosan along with a sol-gel-based molecularly imprinted polymer (MIP) film. The latter was prepared using AM as the template molecule, 3-aminopropyltrimethoxysilane as the functional monomer, and tetraethoxysilane as the cross-linker. The MIP sensor showed a linear current response to the target AM concentration in the range from 0.05 to 5μgmL−1 at a working voltage of 0–0.4V with a lower detection limit of 0.028μgmL−1 (S/N = 3). It was successfully applied to the detection of AM in potato chips. HPLC analysis was also conducted to detect AM in the same samples to demonstrate the applicability of the electrochemical MIP sensor. © 2015, Springer Science+Business Media New York.
语种：
英文
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Effect of metal salts on the immersion method for the preparation of preserved eggs
作者：
刘焱;刘伦伦;周赞;罗灿
期刊：
现代食品科技 ,2015年31(2):196-204 and 231 ISSN：1673-9078
作者机构：
[刘焱; 周赞; 罗灿; 刘伦伦] College of Food Science and Technology, Hunan Agricultural University, Changsha, China;[刘焱] Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha, China
关键词：
清料法;皮蛋;安全性能;感官品质;金属盐
摘要：
以鸭蛋为原料,通过比较不同金属盐清料法加工的皮蛋的安全性能和感官性质,研究了金属盐的种类和添加量对皮蛋品质的影响。试验结果表明：分别添加了Cu SO4、Cu SO4：Zn SO4=1：1和Zn SO4：Fe SO4=1：1腌制的皮蛋的感官品质都较好,评分分别为90.60±2.40、89.80±3.20、88.20±2.70;添加了Zn SO4溶液次之,感官评分为85.80±3.80;单独添加Fe SO4、Mg SO4盐腌制的皮蛋感官品质比较低;添加Cu SO4：Zn SO4=1：1的溶液腌制的皮蛋,锌铜的残留量（铜离子4.12±0.11 mg/kg,锌离子8.32±0.19 mg/kg）比显著低于分别添加锌盐（12.96±0.22 mg/kg）和铜盐（铜离子8.55±0.28 mg/kg）的低皮蛋（P〈0.05）;不同锌盐对皮蛋感官品质的影响有区别,用氧化锌生产的皮蛋锌的残留量（9.27±0.09 mg/kg）和感官评分显著低于其他锌盐腌制的皮蛋,综合考虑,Zn SO4是腌制皮蛋较适合的锌盐;添加Cu SO4或Cu SO4：Zn SO4=1：1生产的皮蛋,蛋黄的溏心指数较高（0.66、0.57）,皮蛋的弹性、硬度、咀嚼度较好,因此,从感官品质和金属的残留量两方面考虑,用Cu SO4和Zn SO4混合清料法腌制皮蛋比较好,而单独添加Fe SO4生产皮蛋明显不适合。
语种：
中文
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Aerobic plate count and bacterial diversity on the surface of eggshells
作者：
陈力力;杨伊磊;青文哲;刘焱;娄爱华
期刊：
现代食品科技 ,2015年31(10):74-79 ISSN：1673-9078
作者机构：
[刘焱; 陈力力; 杨伊磊; 娄爱华] College of Food Science and Technology Hunan Agricultural University, Changsha, China;[刘焱; 陈力力; 青文哲; 娄爱华] Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha, China
关键词：
鸡蛋壳;菌落总数;细菌多样性
摘要：
采用GB 4789.2-2010菌落总数计数及Miseq测序分析物种多样性及群落结构的方法,对蛋鸡场鸡蛋壳表面细菌数量及种群结构进行研究。结果检测8组样品的菌落总数为3.79±0.67~5.06±0.75 Log CFU/eggshell±SD;获得的163214条优化序列分属于变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、梭杆菌门(Fusobacteria)及蓝菌门(Cyanobacteria)。8组样品普遍存在假单胞菌属(Pseudomonas)、埃希氏-志贺氏菌属(Escherichia-Shigella)、泛菌属(Pantoea)、芽孢杆菌属(Bacillus)、肠球菌属(Enterococcus)细菌;而各样品分别以假单胞菌属(Pseudomonas)、埃希氏-志贺氏菌属(Escherichia-Shigella)、不动杆菌属(Acinetobacter)、芽孢杆菌属(Bacillus)、寡养单胞菌属(Stenotrophomonas)以及肠球菌属(Enterococcus)的相对丰度最大,依次为67.89%、36.15%、68.08%、40.12%、21.59%和26.98%。试验结果表明鸡蛋壳表面细菌数量大、种群多,不同样品间存在相似性差异。鸡蛋腐败变质可能与鸡蛋壳表面细菌种群有关,有待进一步研究。
语种：
中文
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Effect of tea polyphenols on grass carp rheological characteristics
作者：
刘泽宇;刘焱;罗灿;朱旗
期刊：
现代食品科技 ,2015年31(6):50-58 ISSN：1673-9078
通讯作者：
Liu, Yan
作者机构：
[刘泽宇] School of Aeronautics, Northwestern Polytechnical University, Xi'an, China;[朱旗] Horticulture and Landscape College, Hunan Agricultural University, Changsha, China;[刘焱] Hunan Province Key Laboratory of Food Science and Biotechnology, Hunan Agricultural University, Changsha, China;[刘焱; 罗灿] College of Food Science and Technology, Hunan Agricultural University, Changsha, China
通讯机构：
College of Food Science and Technology, Hunan Agricultural University, Changsha, China
关键词：
草鱼;茶多酚;乳化性;凝胶强度;流变性
摘要：
采用不同浓度的茶多酚(Tea Polyphenol,TP)处理新鲜草鱼鱼肉,利用分光光度计、质构仪、流变仪及扫描电镜对4℃贮藏的草鱼鱼肉蛋白质的乳化性、凝胶性、流变性及超微结构随贮藏时间的变化进行研究.结果表明,添加0.03％以上TP能延缓草鱼鱼肉蛋白质的乳化性能的降低,提高乳化稳定性;添加0.05％以上TP能增加鱼糜的凝胶强度,降低鱼肉制品的储藏模量和损耗模量,清除由于脂类氧化而产生的自由基,延缓鱼肉蛋白质的降解速度.在试验范围内,TP添加浓度越高,对鱼肉蛋白的流变学特性的保护作用效果越好,但不具备剂量效应,0.05％ TP试验组与0.07％ TP试验组作用效果差异不显著,考虑到生产成本,草鱼冷藏期间建议添加0.05％的TP.
语种：
中文
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茶多酚对氧化脂肪-蛋白质体系的作用研究
作者：
刘焱;刘伦伦;赵晨;瞿朝霞
期刊：
激光生物学报 ,2015年24(5):490-494 ISSN：1007-7146
作者机构：
湖南农业大学食品科学技术学院,湖南 长沙,410128;[赵晨; 瞿朝霞; 刘焱; 刘伦伦] 湖南农业大学
关键词：
草鱼;茶多酚;氧化脂肪;蛋白质
摘要：
目的：研究茶多酚对氧化脂肪-蛋白质体系的作用机理。方法：利用分光光度计和红外光谱检测法对冻藏条件下氧化脂肪-蛋白质模拟体系中肌原纤维蛋白巯基含量、表面疏水性和二级结构进行研究。结果：在冻藏过程中,添加氧化6 h鱼油的肌原纤维蛋白液,巯基含量的减少和表面疏水性的增加最显著,其减少值和增加值分别为28.62 %和53.72 %;同时β-折叠、α-螺旋的减少和β-转角、无规卷曲的增加也最明显。添加0.05%茶多酚的三个组合与其相应的对照组相比,巯基含量的减少、表面疏水性的增加和二级结构的改变都较为缓慢,其中茶多酚抑制巯基含量减少的效果十分显著。结论：氧化鱼油能加速蛋白质的变性,且氧化程度越大,加速效果越明显;而茶多酚能够通过抑制巯基含量的减少来抑制鱼油氧化而导致的蛋白质变性。
语种：
中文
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龙虾副产品酶解调配虾酱工艺条件研究
作者：
娄爱华;刘焱
期刊：
山东化工 ,2015年44(2):27-31+35 ISSN：1008-021X
作者机构：
[娄爱华; 刘焱] 食品科学与生物技术湖南省重点实验室;湖南农业大学食品科学技术学院
关键词：
虾副产品;中性蛋白酶;酶解;调味酱
摘要：
本实验以虾头和虾壳为主要原料,采用酶解预处理制备虾调味酱.在单因素基础上,通过正交实验优化了酶解预处理的工艺条件和虾酱配方.结果表明,酶解预处理的最佳条件为:酶解时间7h,酶添加量3％,酶解温度60℃.在此条件下,氨基氮达34.68ng,/g.虾酱配方比为:酶解液添加量55％,纤维素钠的添加量2.0％,黄酱添加量10％,淀粉添加量7％.在酶解预处理工艺优化下和最佳配方条件下,所制得的虾酱风味鲜美适口,海鲜风味明显,色泽呈棕褐色,稠度较好、无颗粒,不分层.
语种：
中文
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MiR-21 Protected Cardiomyocytes against Doxorubicin-Induced Apoptosis by Targeting BTG2
作者：
Tong, Zhongyi;Jiang, Bimei* ;Wu, Yanyang;Liu, Yanjuan;Li, Yuanbin;...
期刊：
International Journal of Molecular Sciences ,2015年16(7):14511-14525 ISSN：1422-0067
通讯作者：
Jiang, Bimei
作者机构：
[Tong, Zhongyi] Cent S Univ, Xiangya Hosp 2, Dept Pathol, Changsha 410000, Hunan, Peoples R China.;[Xiao, Xianzhong; Li, Yuanbin; Tong, Zhongyi; Liu, Yanjuan; Lv, Qinglan; Jiang, Bimei; Gao, Min; Jiang, Yu] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410000, Hunan, Peoples R China.;[Wu, Yanyang] Hunan Agr Univ, Food Sci & Technol Coll, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Jiang, Bimei] C;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410000, Hunan, Peoples R China.
关键词：
B cell translocation gene 2 (BTG-2);cardiotoxicity;doxorubicin (DOX);microRNA-21 (miR-21)
摘要：
Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of antineoplastic activities. However, it causes cardiac cytotoxicity, and this limits its clinical applications. MicroRNA-21 (miR-21) plays a vital role in regulating cell proliferation and apoptosis. While miR-21 is preferentially expressed in adult cardiomyocytes and involved in cardiac development and heart disease, little is known regarding its biological functions in responding to DOX-induced cardiac cytotoxicity. In this study, the effects of DOX on mouse cardiac function and the expression of miR-21 were examined in both mouse heart tissues and rat H9C2 cardiomyocytes. The results showed that the cardiac functions were more aggravated in chronic DOX injury mice compared with acute DOX-injury mice; DOX treatment significantly increased miR-21 expression in both mouse heart tissue and H9C2 cells. Over-expression of miR-21 attenuated DOX-induced apoptosis in cardiamyocytes whereas knocking down its expression increased DOX-induced apoptosis. These gain- and loss- of function experiments showed that B cell translocation gene 2 (BTG2) was a target of miR-21. The expression of BTG2 was significantly decreased both in myocardium and H9C2 cells treated with DOX. The present study has revealed that miR-21 protects mouse myocardium and H9C2 cells against DOX-induced cardiotoxicity probably by targeting BTG2.
语种：
英文
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不同方法提取的草鱼鱼皮胶原蛋白性质比较
作者：
刘焱;刘伦伦;罗灿;瞿朝霞
期刊：
食品研究与开发 ,2015年(8):34-39 ISSN：1005-6521
作者机构：
湖南农业大学食品科学技术学院，湖南长沙410128;食品科学与生物技术湖南省重点实验室，湖南长沙410128;湖南农业大学食品科学技术学院,湖南长沙,410128
关键词：
鱼皮胶原蛋白;酸法;提取率;电泳
摘要：
采用酸法、碱法、酶法、水法从鱼皮中提取胶原蛋白，对其性质进行分析比较。结果表明，提取率，酸法＞酶法＞碱法＞水法；热变性温度，水法＞酶法＞碱法＞酸法；水法和碱法提取的胶原蛋白SDS-PAGE电泳图谱条带中出现β二聚体和γ三聚体，酶法和酸法没有出现，但是酶法和酸法的α链位置明显色带浓于水法和碱法；紫外吸收光谱中，酸法和碱法的吸收峰强度大于水法和酶法，碱法和酶法左侧出现了杂峰；红外光谱显示，碱法提取的鱼皮胶原蛋白不具备酰胺带特征吸收峰，其二级结构已破坏，酸法、酶法和水法提取的胶原蛋白均存在酰胺带特征吸收峰，而且出现位置相似，符合胶原蛋白特征吸收峰。
语种：
中文
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草鱼鱼鳞、鱼皮和鱼骨酸溶性胶原蛋白特性对比研究
作者：
瞿朝霞;刘焱;罗灿;刘伦伦
期刊：
中国酿造 ,2014年33(5):116-119 ISSN：0254-5071
作者机构：
湖南农业大学食品科学技术学院,湖南长沙,410128;湖南农业大学食品科学技术学院,湖南长沙410128;湖南农业大学食品科学与生物技术湖南省重点实验室,湖南长沙410128
关键词：
草鱼;酸溶性胶原蛋白;电泳;红外光谱
摘要：
从草鱼鱼鳞、鱼皮和鱼骨中提取酸溶性胶原蛋白(ASC),对其特性进行对比分析.紫外光谱和电泳图谱结果显示,3种ASC紫外特征吸收峰出现位置与Ⅰ型胶原蛋白紫外特征吸收峰出现的位置基本一致,分子结构都至少含有两条α链;傅里叶红外光谱显示,草鱼鱼鳞、鱼皮和鱼骨ASC在酰胺A带的N-H伸缩均以氢键形成缔合体,酰胺Ⅰ带和酰胺Ⅲ带的特征吸收峰表明鱼鳞和鱼骨ASC的α-螺旋结构保存较完整,鱼皮ASC的α-螺旋结构已经被破坏;3种ASC在热稳定性方面存在一定差异,热变性温度鱼骨ASC(34.5℃)＞鱼皮ASC (32℃)＞鱼鳞ASC (31℃),可能与胶原存在的组织和所处的环境有关.
语种：
中文
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草鱼冷藏期间ATP关联物含量及新鲜度变化
作者：
刘焱;陈桂平;张继红;卢君
期刊：
食品科学 ,2014年35(12):233-237 ISSN：1002-6630
作者机构：
湖南农业大学食品科学技术学院,湖南长沙 410128;食品科学与生物技术湖南省重点实验室,湖南长沙 410128;湖南农业大学食品科学技术学院,湖南长沙,410128;湖南省食品质量监督检测所,湖南长沙,410002
关键词：
草鱼肉;腺嘌呤核苷三磷酸关联物;核苷酸;鲜度
摘要：
目的:分析草鱼冷藏期间腺嘌呤核苷三磷酸关联物含量的变化,研究草鱼肉中各核苷酸的含量与其新鲜度的相关性。方法:利用强碱将不稳定的核苷酸关联物反应生成其钠盐形式,通过高效液相色谱法检测核苷酸含量,并通过总挥发性盐基氮含量和感官性状的变化进行鲜度评价。结果:生成钠盐形式的腺嘌呤核苷三磷酸关联物能通过色谱很好地分离。新鲜鱼肉中含有高含量的肌苷酸,达504.34 mg/kg。冷藏2 d内草鱼腺嘌呤核苷三磷酸、肌苷酸含量快速下降,次黄嘌呤含量则迅速增加。冷藏后腺嘌呤核苷三磷酸未检出,肌苷酸下降幅度达92.77%,次黄嘌呤增加幅度达89.82%。冷藏期间挥发性盐基氮含量一直上升,冷藏6 d后超出最大值限度,冷藏草鱼的贮藏期限为6 d。
语种：
中文
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