Chemical pre-reduction and electro-reduction guided preparation of a porous graphene bionanocomposite for indole-3-acetic acid detection.
作者：
Su, Zhaohong;Xu, Xiaolin;Cheng, Yongbing;Tan, Yueming;Xiao, Langtao;...
期刊：
Nanoscale ,2019年11(3):962-967 ISSN：2040-3364
通讯作者：
Qin, Xiaoli;Wang, Huixian
作者机构：
[Qin, Xiaoli; Wang, Huixian; Su, Zhaohong; Xu, Xiaolin; Cheng, Yongbing; Tang, Daili; Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Tan, Yueming] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.;[Xiao, Langtao] Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Qin, XL; Wang, HX] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
Acetic acid;Antibodies;Chemical detection;Chemical modification;Cyclic voltammetry;Energy dispersive spectroscopy;Fourier transform infrared spectroscopy;Glass membrane electrodes;Gold nanoparticles;Graphene;High performance liquid chromatography;High resolution transmission electron microscopy;Immunosensors;Nanocomposite films;pH;Polycyclic aromatic hydrocarbons;Reduction;Scanning electron microscopy;Transmission electron microscopy;Ultraviolet visible spectroscopy;Bio-nanocomposite;Chemical impedance spectroscopy;Differential pulse voltammetry;Electrochemical immunosensors;Electrostatic adsorption;Energy dispersive spectroscopies (EDS);Glassy carbon electrodes;Indole-3-acetic acid;Film preparation;gold;graphite;immobilized antibody;indoleacetic acid;indoleacetic acid derivative;metal nanoparticle;nanocomposite;chemistry;electrochemical analysis;electrode;genetic procedures;immunology;limit of detection;oxidation reduction reaction;porosity;static electricity;Antibodies, Immobilized;Biosensing Techniques;Electrochemical Techniques;Electrodes;Gold;Graphite;Indoleacetic Acids;Limit of Detection;Metal Nanoparticles;Nanocomposites;Oxidation-Reduction;Porosity;Static Electricity
摘要：
A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), elecro-chemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2 × 10 -11 to 2 × 10 -8 g mL -1 with a detection limit of 0.016 ng mL -1 (S/N = 3) and showed significant linearity R 2 = 0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranging from -5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite films for wide applications. © 2019 The Royal Society of Chemistry.
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英文
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TBAI-catalyzed selective synthesis of sulfonamides and β-aryl sulfonyl enamines: coupling of arenesulfonyl chlorides and sodium sulfinates with tert-amines
作者：
Jiang, Hongmei;Tang, Xiaoyue;Xu, Zhihui;Wang, Huixian;Han, Kang;...
期刊：
Organic & Biomolecular Chemistry ,2019年17(10):2715-2720 ISSN：1477-0520
通讯作者：
Gui, Qingwen
作者机构：
[Zhou, Yuanyuan; Han, Kang; Tang, Xiaoyue; Yang, Xiaolan; Gui, Qingwen; Wang, Huixian; Feng, Yong-Lai; Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Gui, Qingwen; Xu, Zhihui; Jiang, Hongmei] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yu, Xian-Yong] Hunan Univ Sci & Technol, Minist Educ, Key Lab Theoret Organ Chem & Funct Mol, Taoyuan Rd 9, Xiangtan 411201, Peoples R China.
通讯机构：
[Gui, Qingwen] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
关键词：
Amides;Catalysis;Chlorine compounds;Sodium;Sulfur compounds;C-H bond;Catalyzed oxidation;Enamines;Metal free;Selective synthesis;Sulfinates;Amines
摘要：
A simple, practical and metal-free method has been developed for the synthesis of sulfonamides and β-arylsulfonyl enamines via the selective cleavage of C-N and C-H bonds through the iodine-catalyzed oxidation of arenesulfonyl chlorides and sodium sulfinates with tert-amines. The method uses commercially available inexpensive catalysts and oxidants, and has a wide substrate scope and operational simplicity. © The Royal Society of Chemistry.
语种：
英文
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基于黑米的荧光碳量子点的合成及性能表征
作者：
赖可溱;贺紫萍;布依婷;罗萌;唐湘云;...
期刊：
广州化工 ,2019年47(2):42-44+116 ISSN：1001-9677
作者机构：
湖南农业大学理学院,湖南 长沙,410128;[赖可溱; 贺紫萍; 布依婷; 罗萌; 唐湘云; 苏招红; 蒋红梅] 湖南农业大学
关键词：
碳量子点;黑米;组合法;性能表征;抗阳离子干扰
摘要：
以黑米为碳源,采用热解-溶剂热组合法制备了蓝色荧光碳量子点,经透析纯化后对其性能进行表征.透射电镜表明制备的碳量子点在水溶液中分布均匀且尺寸约为3 nm,紫外-可见光谱表明碳量子点在240 nm和310 nm处有吸收峰,荧光光谱表明碳量子点在440 nm处有最大的发射光谱,且随着激发波长的红移而红移.研究表明该量子点在抗盐性和抗酸碱性方面性能优异.此外,我们还考察了碳量子点对金属离子的响应性能,结果表明该量子点在抗阳离子干扰方面有良好的应用价值.
语种：
中文
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Ultrasound accelerated synthesis of O-alkylated hydroximides under solvent- and metal-free conditions
作者：
Jiang, Hongmei;Tang, Xiaoyue;Liu, Sihan;Wang, Lian;Shen, Haicheng;...
期刊：
Organic & Biomolecular Chemistry ,2019年17(48):10223-10227 ISSN：1477-0520
通讯作者：
Gui, Qing-Wen
作者机构：
[Wang, Lian; Yang, Jiankui; Shen, Haicheng; Gui, Qing-Wen; Liu, Sihan; Wang, Huixian; Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Jiang, Hongmei] Hunan Univ, Coll Chem Engn, State Key Lab ChemoBiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Tang, Xiaoyue] South China Univ Technol, Sch Chem & Chem Engn, Dept Pharmaceut Engn, Guangzhou 510640, Guangdong, Peoples R China.
通讯机构：
[Gui, Qing-Wen] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
关键词：
Catalysts;Conventional heating;Coupling reaction;Cross dehydrogenative couplings;Metal catalyst;Metal-free conditions;N-hydroxyphthalimides;Reaction efficiency;Ultrasound techniques;Ultrasonics
摘要：
A novel, sustainable, environmentally friendly, high substrate scope, efficient, solvent-free and metal catalyst-free method for the cross-dehydrogenative coupling (CDC) reaction between N-hydroxyphthalimide (NHPI) and benzyl/ether compounds is described. This coupling reaction proceeds through ultrasound acceleration. Compared to conventional heating conditions, the use of ultrasound techniques not only improves the reaction efficiency and enhances the reaction rate but also minimizes the side reactions.
语种：
英文
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E-Selective synthesis of vinyl sulfones via silver-catalyzed sulfonylation of styrenes
作者：
Gui, Qingwen;Han, Kang;Liu, Zhuoliang;Su, Zhaohong;He, Xiaoli;...
期刊：
Organic & Biomolecular Chemistry ,2018年16(32):5748-5751 ISSN：1477-0520
通讯作者：
Jiang, Hongmei;Li, Yangyan
作者机构：
[Han, Kang; Su, Zhaohong; Tian, Bufan; Gui, Qingwen; Jiang, Hongmei; He, Xiaoli] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zhuoliang] Natl Univ Def Technol, Coll Liberal Arts & Sci, Changsha 410073, Hunan, Peoples R China.;[Li, Yangyan] Hunan Univ Sci & Engn, Coll Chem & Bioengn, Yongzhou 425199, Peoples R China.
通讯机构：
[Jiang, Hongmei; Li, Yangyan] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;Hunan Univ Sci & Engn, Coll Chem & Bioengn, Yongzhou 425199, Peoples R China.
关键词：
Chemical bonds;Silver;Styrene;Bond coupling;Selective synthesis;SIMPLE protocol;Sulfonylation;Vinyl sulfones;Catalysis
摘要：
An efficient and highly E-selective protocol for the synthesis of vinyl sulfones is described. This simple protocol demonstrates the first synthesis of vinyl sulfones via a silver-catalyzed C-S bond coupling reaction. In addition, the success of the reaction was found to be critically dependent on the use of TEMPO as the additive. © 2018 The Royal Society of Chemistry.
语种：
英文
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Physiological responses and metal uptake of Miscanthus under cadmium/arsenic stress
作者：
Jiang, Hongmei;Zhao, Xiaohui;Fang, Jun* ;Xiao, Yunhua*
期刊：
Environmental Science and Pollution Research ,2018年25(28):28275-28284 ISSN：0944-1344
通讯作者：
Fang, Jun;Xiao, Yunhua
作者机构：
[Xiao, Yunhua; Zhao, Xiaohui; Jiang, Hongmei; Fang, Jun; Xiao, YH] Hunan Agr Univ, Coll Sci, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;[Xiao, Yunhua; Fang, Jun; Xiao, YH] Hunan Engn Lab Pollut Control & waste Utilizat Sw, Changsha 410128, Hunan, Peoples R China.;[Xiao, Yunhua; Fang, Jun; Xiao, YH] Key Lab Rural Ecosyst Hlth Dongting Lake Area Hun, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Fang, J; Xiao, YH] H;[Fang, J; Xiao, YH] K;Hunan Agr Univ, Coll Sci, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.;Hunan Engn Lab Pollut Control & waste Utilizat Sw, Changsha 410128, Hunan, Peoples R China.;Key Lab Rural Ecosyst Hlth Dongting Lake Area Hun, Changsha 410128, Hunan, Peoples R China.
关键词：
Cadmium/arsenic uptake;Hormesis effect;Miscanthus;Physiological indexes
摘要：
The aim of this study is to explore the impact of cadmium (Cd) and arsenic (As) stress on physiological indexes and Cd/As uptake ability of Miscanthus, including Miscanthus sacchariflorus A0104, Miscanthus sinensis C0424 and C0640. Cd and As concentration showed significant hormesis effects on some physiological indexes, such as chlorophyll content, net photosynthetic rate, and MDA content. Compared with control group, Cd uptake can be most greatly enhanced by above fourfold with 150mg/kg Cd treatment. As uptake of A0104 was increased by 3 ~ 33 folds with 200mg/kg As treatment, C0424 was increased by 7–12 folds with 100mg/kg As treatment, while C0640 was increased 1 ~ 6 folds with 250mg/kg As treatment. The results also showed that C0640 was relatively better for the Cd remediation in the high Cd concentration (150mg/kg) contaminated soil, while A0104 and C0424 were relatively better for the As remediation in the high As concentration (100 ~ 200mg/kg) contaminated soil. Additionally, significantly (p < 0.05) close correlations were found among physiological indexes (except MDA content), while physiological indexes showed no significant relationship with the heavy metal contents in root, stem, and leaf. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
语种：
英文
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Enhanced electrocatalytic performance of platinum nanoparticles on thiolated polyaniline-multiwalled carbon nanotubes for methanol oxidation
作者：
Su, Zhaohong* ;Li, Chaorong;Cheng, Yongbing;Gui, Qingwen;Xiong, Yuanfu;...
期刊：
RSC Advances ,2018年8(59):33742-33747 ISSN：2046-2069
通讯作者：
Su, Zhaohong;Jiang, Hongmei;Liu, Xiaoying
作者机构：
[Su, Zhaohong; Liu, Xiaoying; Xiong, Yuanfu; Cheng, Yongbing; Gui, Qingwen; Su, ZH; Jiang, Hongmei; Liu, XY; Li, Chaorong] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Tan, Yueming] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.
通讯机构：
[Su, ZH; Jiang, HM; Liu, XY] H;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.
摘要：
Pt nanoparticles (PtNPs) well-dispersed on thiolated polyaniline (TPANI)-multiwalled carbon nanotubes (MWCNTs) were prepared for enhanced electrocatalytic oxidation of methanol in acidic media. Briefly, the preparation of nanocomposites was carried out via microwave-assisted thiol-ene reaction of 2,5-dimercapto-1,3,4-thiadiazole (DMcT) with oxidized PANI, which was synthesized in the presence of MWCNTs, yielding TPANI-MWCNTs; then, PtNPs were deposited on TPANI-MWCNTs by a microwave-assisted method to obtain PtNPs/TPANI-MWCNT nanohybrids. Fourier transform infrared spectroscopy, cyclic voltammetry (CV), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and inductively coupled plasma-atom emission spectroscopy were used to study relevant nanohybrid properties. TEM showed that PtNPs were well dispersed on TPANI-MWCNTs. TGA showed that PtNPs/TPANI-MWCNTs exhibited better thermal stability than PtNPs/TPANI-MWCNTs and PtNPs/MWCNTs. CV studies showed that PtNPs/TPANI-MWCNT-modified glassy carbon electrode (GCE) exhibited a larger electrochemically active surface area and higher electrocatalytic performance toward methanol electro-oxidation compared with those of PtNPs/PANI-MWCNTs/GCE and PtNPs/MWCNTs/GCE. Also, the PtNPs/TPANI-MWCNTs/GCE electrode possessed high stability and maintained 86% of its initial catalytic activity after 1000-cycle CV in 1.0 M CH 3 OH + 0.5 M H 2 SO 4 . © The Royal Society of Chemistry.
语种：
英文
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黄曲霉及毒素防控技术研究进展
作者：
程翠利;赵小会;蒋红梅;方俊;田云
期刊：
食品工业 ,2018年39(2):296-300 ISSN：1004-471X
作者机构：
湖南农业大学理学院 长沙410128;湖南农业大学生物科学技术学院 长沙410128;[蒋红梅; 田云; 方俊; 赵小会; 程翠利] 湖南农业大学
关键词：
黄曲霉;黄曲霉毒素;防控
摘要：
黄曲霉及其毒素的污染给人类带来巨大经济损失和健康危害,因此寻求对其进行有效防治的策略和技术.目前已研究的多种技术对黄曲霉具有抑制作用或对其毒素具有清除作用,该文综述了利用物理、化学、生物等手段对黄曲霉进行防控的技术研究现状,为黄曲霉深入开展黄曲霉防控技术研究提供参考.
语种：
中文
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农业院校应用化学专业人才培养模式探索与实践
作者：
蒋红梅;杨建奎;喻鹏;李辉勇;王辉宪
期刊：
科教导刊 ,2018年(7):34-35+130 ISSN：1674-6813
作者机构：
湖南农业大学理学院 湖南·长沙 410128;[杨建奎; 蒋红梅; 李辉勇; 喻鹏; 王辉宪] 湖南农业大学
关键词：
综合改革;人才培养;应用化学
摘要：
本文总结了湖南农业大学应用化学专业在综合改革方面的探索与实践。以人才培养模式的改革为契机，对如何将农业院校应用化学类专业办出自己的特色进行了探索，开展了包括：调整专业人才培养定位、人才培养方案修订及人才培养模式的改革等．Y-作；微调了专业口径，设置了专业方向；形成了特色较为鲜明的人才培养模式；取得了较好的改革成效。
语种：
中文
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以蚕沙为碳源合成碳点的方法及其性能表征
作者：
布依婷;徐孝林;程永兵;宋东成;蒋红梅;...
期刊：
广州化工 ,2018年46(17):30-32,39 ISSN：1001-9677
作者机构：
湖南农业大学理学院,湖南 长沙,410128;[蒋红梅; 徐孝林; 宋东成; 布依婷; 程永兵; 苏招红] 湖南农业大学
关键词：
碳点;蚕沙;水热法;直接碳化法;性能表征;Cd2+和Pb2+检测
摘要：
以蚕沙为碳源,经一定条件高温直接碳化或水热碳化,所得的产物经过纯化后得到发蓝色荧光的碳点溶液。通过透射电镜（TEM）,紫外-可见吸收光谱,傅里叶红外光谱（FTIR）等对其性能进行表征。结果表明以蚕沙为原料制备的碳点荧光强度大,表面富含氨基和羟基,具有很强的亲水性,在pH 4~11的范围内和盐溶液中皆具有良好的荧光性能。此外考察了碳点对金属离子的响应性能,结果表明该碳点在选择性检测Cd^2＋和Pb^2＋方面具有潜在的应用价值。
语种：
中文
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自主性学习法在农业院校《基础化学实验》教学中的应用
作者：
苏招红;李辉勇;蒋红梅
期刊：
化工时刊 ,2017年31(8):53-55 ISSN：1002-154X
作者机构：
湖南农业大学理学院,湖南 长沙,410128;[蒋红梅; 李辉勇; 苏招红] 湖南农业大学
关键词：
自主性学习法;基础化学实验;教学研究
摘要：
《基础化学实验》是农业院校全校性非化学专业学生的化学基础课和必修课。我们采用自主性学习法对《基础化学实验》教学进行了探索性改革,通过采用实验记录卡片法、分组讨论法、学生参与实验教学法和创新实验设计法等自主性学习法对实验教学进行了改革,并探讨了《基础化学实验》教学中自主性学习法应用存在的主要问题和对策,以期在《基础化学实验》教学中达到提高学生学习积极性、独立思考能力、分析问题和解决问题的能力、创新能力等目的,最终达到提高教师实验教学质量的目的。
语种：
中文
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Cover Feature: Theoretical Investigation into Suitable Pore Sizes of Membranes for Vanadium Redox Flow Batteries (ChemElectroChem 9/2017)
作者：
Zaichun Liu;Ruilian Li;Jizhong Chen;Dr. Xiongwei Wu;Kai Zhang;...
期刊：
ChemElectroChem ,2017年4(9):2128-2128 ISSN：2196-0216
通讯作者：
Dr. Xiongwei Wu<&wdkj&>Prof. Rudolf Holze<&wdkj&>Prof. Yuping Wu<&wdkj&>Dr. Xiongwei Wu Dr. Xiongwei Wu Dr. Xiongwei Wu<&wdkj&>Prof. Rudolf Holze Prof. Rudolf Holze Prof. Rudolf Holze<&wdkj&>Prof. Yuping Wu Prof. Yuping Wu Prof. Yuping Wu
作者机构：
[Kai Zhang; Jun Mo; Xinhai Yuan; Hongmei Jiang; Kai Zhang Kai Zhang Kai Zhang; Jun Mo Jun Mo Jun Mo; Xinhai Yuan Xinhai Yuan Xinhai Yuan; Hongmei Jiang Hongmei Jiang Hongmei Jiang] College of Science, Hunan Agricultural University, Changsha, Hunan Province, 410128 China;These authors equally contributed to this work;School of Energy Science and Engineering, Institute for Electrochemical Energy Storage, Nanjing Tech University, Nanjing, 211816 Jiangsu Province, China;[Jizhong Chen; Jizhong Chen Jizhong Chen Jizhong Chen] State Key Laboratory of Operation and Control of Renewable Energy & Storage Systems, China;Hunan Province Yinfeng New Energy Co.LTD, Changsha, 410000 China
通讯机构：
[Dr. Xiongwei Wu; Prof. Yuping Wu; Dr. Xiongwei Wu Dr. Xiongwei Wu Dr. Xiongwei Wu; Prof. Yuping Wu Prof. Yuping Wu Prof. Yuping Wu] C;[Prof. Rudolf Holze; Prof. Rudolf Holze Prof. Rudolf Holze Prof. Rudolf Holze] T;College of Science, Hunan Agricultural University, Changsha, Hunan Province, 410128 China<&wdkj&>Hunan Province Yinfeng New Energy Co.LTD, Changsha, 410000 China<&wdkj&>Technische Universität Chemnitz, Institut für Chemie, 09107 Chemnitz, Germany<&wdkj&>College of Science, Hunan Agricultural University, Changsha, Hunan Province, 410128 China<&wdkj&>School of Energy Science and Engineering, Institute for Electrochemical Energy Storage, Nanjing Tech University, Nanjing, 211816 Jiangsu Province, China<&wdkj&>Hunan Province Yinfeng New Energy Co.LTD, Changsha, 410000 China
关键词：
vanadium redox flow batteries;ion-exchange membranes;density functional calculations;pore size
语种：
英文
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脉冲电位法制备碳量子点及性能表征
作者：
宋东成;黄昭帅;程永兵;徐孝林;蒋红梅;...
期刊：
广州化学 ,2017年42(5):36-40 ISSN：1009-220X
作者机构：
湖南农业大学理学院,湖南长沙,410128;[蒋红梅; 徐孝林; 宋东成; 黄昭帅; 程永兵; 苏招红] 湖南农业大学
关键词：
碳量子点;乙醇;邻苯二胺;脉冲电位法;性能表征
摘要：
以乙醇为碳源,邻苯二胺为掺杂剂,采用脉冲电位法制备了发黄色荧光的碳量子点,并对其性能进行了表征。透射电镜表明制备的碳量子点尺寸在5 nm以内,紫外光谱表明碳量子点有两个吸收峰,荧光光谱表明所制备的碳量子点在552 nm处具有独立于激发波长的发射峰,且具有较好的抗盐性和抗酸碱性。此外考察了碳量子点对金属离子的响应性能,结果表明该碳量子点在选择性检测Fe~（3＋）方面具有潜在的应用价值。
语种：
中文
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麝香草酚对13种植物病原微生物的抑制作用
作者：
程翠利;韩康;卢向阳;方俊;田云;...
期刊：
安徽农业科学 ,2017年45(24):144-145 ISSN：0517-6611
作者机构：
湖南农业大学理学院,湖南长沙,410128;湖南农业大学生物科学与技术学院,湖南长沙,410128;[蒋红梅; 田云; 卢向阳; 韩康; 方俊; 程翠利] 湖南农业大学
关键词：
麝香草酚;病原微生物;最低抑菌浓度(MIC);最低杀菌浓度(MBC)
摘要：
[目的]探讨石香薷主要成分麝香草酚对植物病原菌的影响。[方法]采用管碟法研究麝香草酚对13种致病微生物的抑制作用。[结果]麝香草酚对13种受试菌均有不同程度的抑制作用,其中对水稻恶苗病菌、黄瓜枯萎病菌、黄瓜炭疽病菌、油菜菌核病菌4种植物病害真菌的抑制效果极显著,抑菌效果与麝香草酚浓度呈正相关,对这4种真菌的最低抑菌浓度（MIC）分别为0.125、0.063、0.063、0.250 mg/mL,最低杀菌浓度（MBC）分别为0.125、0.125、0.063、3.000 mg/mL。[结论]试验结果可为麝香草酚的进一步研究提供理论依据。
语种：
中文
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Theoretical Investigation into Suitable Pore Sizes of Membranes for Vanadium Redox Flow Batteries
作者：
Liu, Zaichun;Li, Ruilian;Chen, Jizhong;Wu, Xiongwei* ;Zhang, Kai;...
期刊：
ChemElectroChem ,2017年4(9):2184-2189 ISSN：2196-0216
通讯作者：
Wu, Xiongwei;Wu, Yuping;Holze, Rudolf
作者机构：
[Li, Ruilian; Liu, Zaichun; Yuan, Xinhai; Wu, Xiongwei; Mo, Jun; Wu, XW; Wu, Yuping; Zhang, Kai; Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Liu, Zaichun; Wu, Yuping] Nanjing Tech Univ, Inst Electrochem Energy Storage, Sch Energy Sci & Engn, Nanjing 211816, Jiangsu, Peoples R China.;[Chen, Jizhong] State Key Lab Operat & Control Renewable Energy &, Beijing, Peoples R China.;[Wu, Xiongwei; Wu, XW; Wu, Yuping] Hunan Prov Yinfeng New Energy Co LTD, Changsha 410000, Hunan, Peoples R China.;[Holze, Rudolf] Tech Univ Chemnitz, Inst Chem, D-09107 Chemnitz, Germany.
通讯机构：
[Wu, XW; Wu, YP] H;[Wu, Yuping] N;[Holze, Rudolf] T;Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;Nanjing Tech Univ, Inst Electrochem Energy Storage, Sch Energy Sci & Engn, Nanjing 211816, Jiangsu, Peoples R China.
关键词：
vanadium redox flow batteries;ion-exchange membranes;density functional calculations;pore size
摘要：
<jats:title>Abstract</jats:title><jats:p>For the first time, a three‐dimensional (3D) volume calculation model based on total electrostatic potential analysis and density functional calculations is applied to study the volume differences between hydrated multivalent vanadium ions (V<jats:sup>2+</jats:sup>, V<jats:sup>3+</jats:sup>, VO<jats:sup>2+</jats:sup>, and <jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/celc201700244-math-0001.png" xlink:title="urn:x-wiley:21960216:media:celc201700244:celc201700244-math-0001" />
) and charge‐balancing ions (H<jats:sub>3</jats:sub>O<jats:sup>+</jats:sup>, <jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/celc201700244-math-0002.png" xlink:title="urn:x-wiley:21960216:media:celc201700244:celc201700244-math-0002" />
and <jats:inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/celc201700244-math-0003.png" xlink:title="urn:x-wiley:21960216:media:celc201700244:celc201700244-math-0003" />
) encountered in the electrolyte solutions of vanadium redox flow batteries (VRBs). The calculated results indicate that radii of all charge‐balancing ions are less than 3.01 Å and of all hydrated multivalent vanadium ions are greater than 3.78 Å. The results of our calculations also suggest that cation‐exchange membranes with pore sizes ranging from 3.98 to 7.56 Å and anion‐exchange membranes with pore sizes ranging from 6.02 to 7.56 Å are suitable for the VRB application. These computational results agree very well with reported experimental results and provide valuable guidance for the selection and design of membranes on the molecular level for VRB applications.</jats:p>
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英文
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10％精喹禾灵乳油对斑马鱼的急性毒性试验
作者：
赵小会;程翠利;周春姣;蒋红梅
期刊：
广州化工 ,2016年(16):108-110 ISSN：1001-9677
作者机构：
湖南农业大学理学院,湖南长沙,410128
关键词：
精喹禾灵;斑马鱼;急性毒性;致死中浓度;高效液相色谱
摘要：
农业生产中粮食产量的提高离不开农用除草剂的施用,而施用除草剂的过程中难免会造成环境的污染,进而使环境生物受到迫害。以斑马鱼作为试验用鱼,采用半静态法测定10%精喹禾灵乳油对鱼类的急性毒性。将斑马鱼置于配制好的精喹禾灵药液中进行染毒,利用高效液相色谱法测定斑马鱼经0 h、24 h、48 h、72 h、96 h染毒后水体实际浓度,记录相应浓度下斑马鱼的死亡数目,计算各时间点的致死中浓度,并观察斑马鱼中毒症状。根据化学农药毒性等级划分标准进行分级,确定10%精喹禾灵乳油农药的毒级为高毒。
语种：
中文
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黄龙胆根黄酮类化合物的提取、纯化工艺研究
作者：
刘丽华;周春娇;蒋红梅;王辉宪;杨华武;...
期刊：
广州化工 ,2016年(5):124-128+137 ISSN：1001-9677
作者机构：
[刘丽华; 周春娇; 蒋红梅; 王辉宪] 湖南农业大学理学院;[杨华武; 赵国玲] 湖南中烟工业有限责任公司
关键词：
黄龙胆;黄酮类化合物;微波辅助法;提取;纯化;大孔树脂
摘要：
通过单因素与正交实验研究了微波辅助乙醇溶剂法提取黄龙胆根黄酮类化合物的最佳工艺条件,优化了大孔树脂对所得黄酮类化合物进行纯化的工艺。得出提取最佳工艺条件为:微波功率300 W,微波时间8 min,乙醇浓度35%,料液比1∶20。在此条件下,得率为32.19%,粗提物总黄酮含量为1.97%。通过静态实验,选出最佳纯化树脂为AB-8大孔树脂;通过动态实验,得出吸附分离黄龙胆根黄酮类化合物工艺参数为:上样液流速2.00 m L/min,洗脱剂流速2.00 m L/min。经纯化后总黄酮含量为20.17%。
语种：
中文
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A robust analytical method for measurement of phytoestrogens and related metabolites in serum with liquid chromatography tandem mass spectrometry
作者：
Jiang, Hongmei;Liao, Xiangjun;Wood, Carla M.;Xiao, Chao-Wu* ;Feng, Yong-Lai*
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES ,2016年1012-1013:106-112 ISSN：1570-0232
通讯作者：
Feng, Yong-Lai;Xiao, Chao-Wu
作者机构：
[Jiang, Hongmei] Hunan Agr Univ, Coll Sci, Changsha 410128, Hunan, Peoples R China.;[Feng, Yong-Lai; Jiang, Hongmei; Liao, Xiangjun] Hlth Canada, Exposure & Biomonitoring Div, Environm Hlth Sci & Res Bur, Hlth Environm & Consumer Safety Branch, 50 Columbine Driveway,AL 0800C, Ottawa, ON K1A 0K9, Canada.;[Wood, Carla M.; Xiao, Chao-Wu; Jiang, Hongmei] Hlth Canada, Hlth Prod & Food Branch, Nutr Res Div, 251 Sir Frederick Banting Driveway, Ottawa, ON K1A 0K9, Canada.
通讯机构：
[Feng, Yong-Lai; Xiao, Chao-Wu] H;Hlth Canada, Exposure & Biomonitoring Div, Environm Hlth Sci & Res Bur, Hlth Environm & Consumer Safety Branch, 50 Columbine Driveway,AL 0800C, Ottawa, ON K1A 0K9, Canada.;Hlth Canada, Hlth Prod & Food Branch, Nutr Res Div, 251 Sir Frederick Banting Driveway, Ottawa, ON K1A 0K9, Canada.
关键词：
Biomolecules;Body fluids;Chromatography;Column chromatography;Flavonoids;High performance liquid chromatography;High pressure liquid chromatography;Liquids;Mass spectrometry;Metabolites;Rats;Shells (structures);Signal to noise ratio;Spectrometry;Core shell;High-performance liquid chromatography tandem mass spectrometries;Phytoestrogens;Rapid separation;Rat serum;Liquid chromatography;beta glucuronidase;casein;equol;glucuronidase H 5;glycitein;isoflavone derivative;phytoestrogen;soybean protein;sulfatase;sulfatase H 1;unclassified drug;phytoestrogen;animal experiment;Article;calibration;controlled study;female;Helix pomatia;high performance liquid chromatography;hormone determination;hormone release;limit of detection;metabolomics;nonhuman;priority journal;quantitative analysis;rat;signal noise ratio;tandem mass spectrometry;animal;blood;chemistry;high performance liquid chromatography;isolation and purification;procedures;Sprague Dawley rat;tandem mass spectrometry;Animals;Chromatography, High Pressure Liquid;Female;Limit of Detection;Phytoestrogens;Rats;Rats, Sprague-Dawley;Tandem Mass Spectrometry
摘要：
A sensitive and robust method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for quantitation of 13 phytoestrogens and related metabolites in rat serum samples. A new type of column, the Kinetex core-shell C18 column, was applied for rapid separation of the target analytes in 10 min. Two enzymes, sulfatase H-1 and gulcuronidase H-5 from Helix pomatia were compared on the efficiency of releasing the conjugated forms of the target analytes to their free forms in serum samples. The method detection limit (MDL) defined as three times the signal to noise ratio in spiked serum matrix-based solutions was in the range of 0.1-3.5 ng/mL. The linear dynamic calibration was in the broad range of 0.2-500 ng/mL for all target compounds. Thirty-two rat serum samples from the rats that were fed with diets containing either casein or soy protein isolates with various amounts of isoflavones for 8 weeks were analyzed for the target analytes with the developed method. Nine target analytes were detected in the serum samples. Those detectable compounds are all the metabolites of the dietary isoflavones, suggesting that the diet isoflavones were mostly metabolized to their metabolites in rat. © 2016 Published by Elsevier B.V. All rights reserved.
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英文
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橙皮多糖组成、理化性质及抗氧化性检测
作者：
乔立龙;程翠利;周春姣;王辉宪;蒋红梅
期刊：
广州化工 ,2016年(1):125-128 ISSN：1001-9677
作者机构：
湖南农业大学理学院,湖南 长沙,410128;[蒋红梅; 乔立龙; 周春姣; 程翠利; 王辉宪] 湖南农业大学
关键词：
多糖;橙皮;组成;理化性质;抗氧化能力
摘要：
探索并优化了橙皮多糖的提取工艺条件,检测纯化后橙皮多糖的组成,理化性质及抗氧化性；方法：采用用单因素及正交实验设计探索并优化超声波辅助提取橙皮多糖的工艺条件,粗多糖经氯仿：正丁醇(5：1, V：V)去蛋白、浓缩、乙醇沉淀后冷冻干燥,利用离子色谱技术分析纯化后橙皮多糖的组成,采用常规理化检测方法及体外抗氧化实验检测了纯化后橙皮多糖的理化性质及抗氧化性能力。结果表明离子色谱法测得橙皮多糖由3种单糖组成,分别为鼠李糖、葡萄糖、半乳糖醛酸；理化性质实验结果显示,橙皮多糖溶于水,微溶于体积分数为95%乙醇,不溶于乙醚、丙酮,灰分含量为0.1191%,蛋白质含量为0.15 mg/g抗氧化能力低于抗坏血酸(Vc)和2,6-二叔丁基对甲酚(BHT),橙皮粗多糖抗氧化能力优于精制橙皮多糖。
语种：
中文
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粗饲料体外发酵特性及对微生物细胞膜界面物理化学特性的影响
作者：
党坦;方俊;蒋红梅;谭支良;汤少勋
期刊：
动物营养学报 ,2015年27(1):124-132 ISSN：1006-267X
作者机构：
湖南农业大学理学院,长沙,410128;[方俊] 湖南农业大学生命科学与技术学院,长沙,410128;中国科学院亚热带农业生态研究所，中国科学院农业生态过程重点实验室，长沙 410125;[蒋红梅; 党坦] 湖南农业大学;[汤少勋; 谭支良] 中国科学院亚热带农业生态研究所
关键词：
粗饲料;体外发酵特性;瘤胃微生物;界面物理化学特性
摘要：
本试验旨在研究不同来源粗饲料发酵特性及对微生物细胞膜界面物理化学特性的影响。以小麦秸、玉米秸、水稻秆、苜蓿和桂牧一号为试验材料,采用体外发酵技术,研究不同粗饲料发酵48 h时体外发酵特性及发酵液微生物界面物理化学特性。结果表明:1)5种粗饲料体外发酵产气量及产气动力学参数[最大产气量(Vf)、产气曲线常数(b)、产气分率(k)、达到最大产气量1/2时所用时间(T_(0.5))、初始发酵产气分率(FRD_0)和达到最大产气量1/2时的发酵产气分率(FRDt_(0.5))]存在差异;Vf以桂牧一号最高,显著高于水稻秆(P<0.05),极显著高于小麦秸(P<0.01);b、k及T_(0.5)以小麦秸最高,T_(0.5)按小麦秸>水稻秆>玉米秸和桂牧一号>苜蓿的顺序显著降低(P<0.05);苜蓿的FRD_0和FRDt_(0.5)显著或极显著高于桂牧一号、水稻秆和玉米秸(P<0.05或P<0.01)。2)苜蓿干物质消失率显著或极显著高于水稻秆、小麦秸(P<0.05或P<0.01),氨态氮含量显著高于桂牧一号、水稻秆(P<0.05)。不同粗饲料间发酵液微生物细胞膜通透性按玉米秸>小麦秸>水稻秆>桂牧一号和苜蓿的顺序显著降低(P<0.05)。由此可见,不同品种粗饲料间在发酵特性以及微生物部分界面物理化学特性间存在差异,利用不同粗饲料发酵不同步的特性进行组合,可能有利于提高粗饲料的利用。
语种：
中文
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