作者： 易锦辉;周丽媛;宋泽和;贺喜

期刊： 动物营养学报,2020年32(08):3509-3515 ISSN：1006-267X

作者机构： 湖南农业大学动物科学技术学院,长沙 410128;湖南家禽安全生产工程技术研究中心,湖南畜禽安全生产协同创新中心,长沙 410128

关键词： 牛蒡子苷;药理活性;抗氧化;抗炎;抗菌

摘要： 牛蒡子苷是从中药牛蒡子中分离的木脂素,是牛蒡子中主要的活性成分,已经发现牛蒡子苷具有抗炎、免疫调节、抗病毒、抗肿瘤等活性,其在医学上对于糖尿病、肾病和部分神经退行性疾病也有一定的疗效。本文综述了最近几年国内外对于牛蒡子苷的药理作用以及生物活性的研究,并探讨了牛蒡子苷作为一种饲用资源的应用前景,以期为合理开发应用牛蒡子苷资源提供理论参考。

语种： 中文

作者： Lin, Qian;Peng, Simin;Li, Yinghui;Jiang, Guitao;Liao, Zhenzhang;...

期刊： Animal Science Journal,2020年91(1):e13301- ISSN：1344-3941

通讯作者： Dai, Qiuzhong;He, Xi

作者机构： [Lin, Qian; Jiang, Guitao; Liao, Zhenzhang; Dai, Qiuzhong] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;[Lin, Qian] Changsha Univ, Dept Biol & Environm Engn, Changsha, Hunan, Peoples R China.;[Lin, Qian] Hunan Deren Husb Technol Co Ltd, Changde, Peoples R China.;[Peng, Simin] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Li, Yinghui; He, Xi; Fan, Zhiyong; Liao, Zhenzhang] Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Dai, Qiuzhong] C;[He, Xi] H;Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.;Hunan Agr Univ, Coll Anim Sci & Technol, Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.

关键词： antioxidant status;biochemical characteristics;carcass and meat quality;duck;Magnolol

摘要： Magnolol rich in Magnolia officinalis is a bioactive polyphenolic compound. The aim of this study was to clarify the effects of magnolol additive (MA) on carcass and meat quality, biochemical characteristics and antioxidative capacity of Linwu ducks, by comparing it to that of antibiotic additive (colistin sulphate, CS). A total of 275 49-d-old ducks were randomly assigned to 5 groups with 5 cages of 11 ducks each and fed by the diets supplemented with 0, 100, 200 and 300 mg of MA/kg and 30 mg of CS/kg for 3 weeks, respectively. The results revealed that MA administration not only increased dressed percentage (calculated as a percentage of live weight), percentage of breast muscle, leg muscle and lean meat (calculated as a percentage of eviscerated weight), but also remarkably increased a*45 min and pH45 min of leg muscle. Moreover, MA administration decreased the percentage of abdominal fat (calculated as a percentage of eviscerated weight), 45-min cooking loss, water loss rate of leg muscle, 45-min cooking loss and drip loss of breast muscle at 24 hr and 48 hr. Furthermore, MA administration enhanced the activities of superoxide dismutase and catalase in serum or liver, serum total antioxidant capacity and hepatic reduced glutathione concentration significantly, compared with the basal diet or CS group (p < .05). On the other hand, triglyceride, total cholesterol, aspartate aminotransferase, malondialdehyde, protein carbonyl and 8-hydroxy-2'-deoxyguanosine contents in serum and liver were significantly increased in Linwu ducks fed with CS, compared with MA groups (p < .05). Taken together, these data demonstrated that magnolol could effectively improve the carcass and meat quality of Linwu ducks by regulating the in vivo antioxidant status and would be a potential candidate to replace antibiotic.

语种： 英文

作者： Xie, Kun;He, Xi;Chen, Keyu;Sakao, Kozue;Hou, De-Xing*

期刊： FOOD & FUNCTION,2020年11(7):5976-5991 ISSN：2042-6496

通讯作者： Hou, De-Xing

作者机构： [Sakao, Kozue; Xie, Kun; Hou, De-Xing; Chen, Keyu] Kagoshima Univ, United Grad Sch Agr Sci, Course Biol Sci & Technol, Kagoshima, Japan.;[He, Xi] Hunan Agr Univ, Hunan Engn Res Ctr Poultry Safety, Coll Anim Sci & Technol, Changsha, Peoples R China.;[Sakao, Kozue; Hou, De-Xing] Kagoshima Univ, Dept Food Sci & Biotechnol, Fac Agr, Kagoshima, Japan.

通讯机构： [Hou, De-Xing] K;Kagoshima Univ, United Grad Sch Agr Sci, Course Biol Sci & Technol, Kagoshima, Japan.;Kagoshima Univ, Dept Food Sci & Biotechnol, Fac Agr, Kagoshima, Japan.

摘要： Non-alcoholic fatty liver disease (NAFLD) is a disease that is prevalent worldwide, and its prevention by dietary administration has recently been considered as an important strategy. In this study, we administered mice with vine tea polyphenol (VTP) extracted from Ampelopsis grossedentata, a Chinese herb, to investigate the preventive effect on western diet (WD)-induced NAFLD. Male C57BL/6N mice were fed either a normal diet (ND) or WD with or without VTP for 12 weeks. The results revealed that VTP supplementation decreased the serum levels of cholesterol and triglycerides, and reduced the accumulation of hepatic lipid droplets caused by WD. Molecular data revealed that VTP enhanced fatty acid oxidation by reactivating the WD-suppressed phosphorylation of AMP-activated protein kinasea (AMPKa) and the expressions of peroxisome proliferator-activated receptor alpha (PPARa), carnitine palmitoyl transferase IA (CPT1A) and cytochrome P450, family 4, subfamily a1 (CYP4A1). VTP inhibited hepatic lipogenesis by reducing the WD-enhanced level of mature sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS). Moreover, VTP activated nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-mediated expressions of hemeoxygenase-1 (HO-1) and quinone oxidoreductase (NQO1), and reduced hepatic TBARS levels to prevent hepatic oxidative stress. On the other hand, VTP also increased intestinal zonula occludens-1 (ZO-1) expression and the relative abundance of gut Akkermansia, and reduced the ratio of Firmicutes/Bacteroidetes. Thus, VTP might prevent WD-induced NAFLD by balancing fatty acid oxidation and lipogenesis, hepatic oxidative stress, and gut microbiome, at least. These results suggest that vine tea, containing a high content of the bioactive compound dihydromyricetin, is a potential food resource for preventing NAFLD. © 2020 The Royal Society of Chemistry.

语种： 英文

作者： 单红艳;丁鹏;贺喜

期刊： 湖南饲料,2019年(3):38-44 ISSN：1673-7539

作者机构： 湖南农业大学动物科学技术学院,饲料安全与高效利用教育部工程研究中心,湖南畜禽安全生产协同创新中心,长沙 410128;[贺喜; 丁鹏; 单红艳] 湖南农业大学

关键词： 芽孢杆菌;生理作用;猪;肉鸡;蛋鸡

摘要： 芽孢杆菌作为常见的益生菌品种之一,在畜禽日粮中的应用研究已经比较广泛,本文就芽孢杆菌的生理作用及其在猪鸡上的应用研究进展进行了综述,旨在为芽孢杆菌在生产上的应用提供理论指导。

语种： 中文

作者： 李浩;丁亚南;宋泽和;贺喜;范志勇

期刊： 经济动物学报,2019年23(03):175-180,186 ISSN：1007-7448

作者机构： 湖南农业大学动物科学技术学院,长沙 410128;湖南畜禽安全生产协同创新中心,长沙410128;[李浩; 丁亚南; 贺喜; 范志勇; 宋泽和] 湖南畜禽安全生产协同创新中心

关键词： 饲粮纤维;发酵;繁殖性能;母猪

摘要： 饲粮纤维是饲料中不被动物自身消化酶所消化的一类物质,是由抗性淀粉、纤维素、半纤维素、果胶、木质素等成分组成的复合物。饲粮纤维在肠道中的发酵是发挥其营养功能的关键,这与其水合特性密切相关。在妊娠阶段合理运用饲粮纤维可起到调节母猪体况、提高产仔规模与增加断奶窝重的效果。文中对饲粮纤维的成分、来源及理化特性及其在母猪饲粮中的应用效果进行综述,旨在为富含纤维的饲用资源在母猪饲粮中的高效利用提供参考。

语种： 中文

作者： 丁鹏;黄祥祥;宋泽和;范志勇;呙于明;...

期刊： 动物营养学报,2019年31(3):1138-1151 ISSN：1006-267X

作者机构： [贺喜; 丁鹏; 范志勇; 宋泽和; 黄祥祥] 湖南农业大学动物科学技术学院,湖南家禽安全生产工程技术研究中心,饲料安全与高效利用教育部工程研究中心,湖南畜禽安全生产协同创新中心,长沙 410128;中国农业大学动物科学技术学院,动物营养学国家重点实验室,北京 100193;[呙于明] 中国农业大学

关键词： 肉仔鸡;菜籽粕;日龄;氨基酸消化率;预测方程

摘要： 本试验旨在分析湖南省6种不同地区菜籽粕的18种氨基酸含量,测定14和28日龄肉仔鸡对6种不同地区菜籽粕中氨基酸的标准回肠消化率,并建立赖氨酸、蛋氨酸、色氨酸及苏氨酸标准回肠消化率的预测方程.试验共选取700羽体重和健康状况一致的1日龄爱拔益加(AA)肉仔鸡,根据日龄分别进行2个阶段的试验.第1阶段(7 ~ 14日龄) :将420羽肉仔鸡随机分为7个处理,每个处理10个重复,每个重复6羽;第2阶段(21 ~ 28日龄) :将280羽肉仔鸡随机分为7个处理,每个处理10个重复,每个重复4羽.2个阶段中的7个处理分别为6个不同地区的菜籽粕采用全替代法设计的试验饲粮组和1个无氮饲粮组.分别在14和28日龄收集试验鸡回肠食糜用于测定菜籽粕氨基酸标准回肠消化率并通过回归分析建立预测方程.结果显示: 14和28日龄肉仔鸡对菜籽粕中18种氨基酸标准回肠消化率的平均值波动范围分别为63.26%~ 79.58%和66.22%~ 80.34%.14日龄肉仔鸡对菜籽粕中赖氨酸、蛋氨酸、苏氨酸和色氨酸标准回肠消化率的预测方程分别为Lys = - 90.81 + 4.562CP(R~2 = 0.792,P < 0.05) 、Met = 110.644-2.810CF(R~2 = 0.845,P<0.05) 、Trp = 106.834-0.725NDF(R~2 = 0.989,P<0.05)和Thr = -79.357+4.284CP(R~2 = 0.715,P<0.05) ; 28日龄肉仔鸡对菜籽粕中赖氨酸、蛋氨酸、苏氨酸和色氨酸标准回肠消化率的预测方程分别为Lys = 161.02 - 2.002NDF(R~2 = 0.957,P< 0.05) 、Met = 144.269-1.521NDF(R~2 = 0.831,P<0.05) 、Trp = 112.053-0.824NDF(R~2 = 0.999,P<0.05)和Thr = 99.983-2.566CF(R~2 = 0.712,P<0.05) .上述预测方程中: Lys为赖氨酸标准回肠消化率; Met为蛋氨酸标准回肠消化率; Trp为色氨酸标准回肠消化率; Thr为苏氨酸标准回肠消化率; CP为粗蛋白质含量; CF为粗纤维含量; NDF为中性洗涤纤维含量.本试验通过分析粗蛋白质、粗纤维及中性洗涤纤维含量与氨基酸标准回肠消化率的相关关系,建立了14和28日龄肉仔鸡对湖南省菜籽粕中赖氨酸、蛋氨酸、色氨酸和苏氨酸标准回肠消化率的预测方程,可为菜籽粕在肉仔鸡饲粮中的高效利用提供便利.

语种： 中文

作者： 罗艳辉;贺喜;宋泽和;范志勇

期刊： 广东饲料,2019年28(8):40-43 ISSN：1005-8613

作者机构： [贺喜; 范志勇; 宋泽和] 湖南农业大学动物科学技术学院;[贺喜; 范志勇; 宋泽和] 饲料安全与高效利用教育部工程研究中心;[贺喜; 范志勇; 宋泽和] 湖南畜禽安全生产协同创新中心;[罗艳辉] 湖南农业大学

关键词： 发酵桑叶;营养价值;青贮调制;饲用

摘要： 桑叶通过发酵在一定程度上可减少粗纤维和有关抗营养因子的含量,提高蛋白质含量,从而具有更高的营养价值和饲用价值。本文主要介绍了发酵桑叶的营养价值、发酵菌种选择、发酵条件控制和桑叶青贮的研究进展,并总结了发酵桑叶在畜禽以及鱼类生产中的应用,旨在为桑叶资源的进一步开发提供科学参考。

语种： 中文

作者： 孙鑫东;田莎;赵月香;吕宏伟;李瑞;...

期刊： 动物营养学报,2019年31(4):1781-1788 ISSN：1006-267X

作者机构： [贺喜; 李瑞; 田莎; 范志勇; 赵月香; 宋泽和; 吕宏伟; 孙鑫东] 湖南农业大学动物科学技术学院,饲料安全与高效利用教育部工程研究中心,湖南畜禽安全生产协同创新中心,长沙 410128

关键词： 生长猪;全粒木薯;消化能;代谢能;回归法

摘要： 本试验旨在分析和测定全粒木薯的常规养分及其生长猪消化能(DE) 、代谢能(ME) 。试验选用体重为(51.9±1.8) kg的“杜×长×大”三元杂交健康去势公猪12头,采用交叉设计并分配3种饲粮。饲粮设计采取等比回归法,包括1组玉米-豆粕型基础饲粮和2组由全粒木薯分别等比替代15%、30%基础饲粮的试验饲粮,所有饲粮中除待测原料外,其余能量原料间均保持着相同的比例;回归过程以全粒木薯摄入量为回归因子,对生长猪DE、ME摄入量作回归方程,进而得到全粒木薯生长猪DE、ME。结果表明: 1)全粒木薯总能为15.02 MJ/kg,干物质含量为88.58%,粗蛋白质含量为2.62%,粗脂肪含量为0.62%,粗纤维含量为17.66%,粗灰分含量为7.97%,淀粉含量为45.16%。2)基础饲粮组DE显著高于30%全粒木薯替代饲粮组(P<0.05),但与15%全粒木薯替代饲粮组差异不显著(P>0.05);基础饲粮组ME显著高于30%全粒木薯替代饲粮组(P<0.05),但与15%全粒木薯替代饲粮组差异不显著(P>0.05); 15%与30%全粒木薯替代饲粮组间DE差异显著(P<0.05),但15%与30%全粒木薯替代饲粮组间ME差异不显著(P> 0.05) 。3)全粒木薯摄入量与其生长猪DE、ME摄入量关系的回归方程分别为: Y = 0.61+12.50X (R~2 = 0.96,CV= 0.17),Y = 0.16+11.82X(R~2 = 0.93,CV= 0.23) 。综上所述,全粒木薯能量利用价值一般,其生长猪DE、ME分别为12.50、11.82 MJ/kg DM,建议用其替代玉米-豆粕型饲粮的最佳水平为15%,不影响生长猪DE、ME,但还需平衡饲粮中氨基酸水平。

语种： 中文

作者： Xie, Kun;He, Xi;Chen, Keyu;Chen, Jihua;Sakao, Kozue;...

期刊： Antioxidants,2019年8(8) ISSN：2076-3921

通讯作者： Hou, De-Xing

作者机构： [Sakao, Kozue; Xie, Kun; Hou, De-Xing; Chen, Keyu] Kagoshima Univ, United Grad Sch Agr Sci, Biol Sci & Technol, Kagoshima 8900065, Japan.;[He, Xi] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Chen, Jihua] Cent S Univ, Xiangya Sch Publ Hlth, Changsha 410128, Hunan, Peoples R China.;[Sakao, Kozue; Hou, De-Xing] Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima 8900065, Japan.

通讯机构： [Hou, De-Xing] K;Kagoshima Univ, United Grad Sch Agr Sci, Biol Sci & Technol, Kagoshima 8900065, Japan.;Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima 8900065, Japan.

关键词： Ampelopsis grossedentata;Nrf2/Keap1;antioxidant ability;dihydromyricetin

摘要： Ampelopsis grossedentata, also called vine tea, has been used as a traditional beverage in China for centuries. Vine tea contains rich polyphenols and shows benefit to human health, but the chemical and antioxidant properties of vine tea polyphenols from different locations remain unclear. This study aims to investigate the chemical and antioxidant properties of vine tea from three major production areas in China including Guizhou, Hunan, and Guangxi Provinces. The highest amount of polyphenol from vine tea was extracted by 70% ethanol at 70 °C for 40 min with ultrasonic treatment. The major compound in vine tea polyphenols (VTP) was determined as dihydromyricetin (DMY) by high-performance liquid chromatography (HPLC) and the content was estimated as 21.42%, 20.17%, and 16.47% of dry weight basis from Hunan, Guizhou, and Guangxi products, respectively. The antioxidant activities were investigated in vitroand in culture hepatic cells. VTP and DMY showed strong 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH) scavenging ability and high oxygen radical absorption capacity (ORAC) value in vitro. VTP and DMY also increased the level of nicotinamide adenine dinucleotide phosphate (NADPH):quinone oxidoreductase (NQO1) in HepG2 cells. Moreover, VTP and DMY enhanced the level of nuclear factor erythroid 2-related factor 2 (Nrf2) and reduced the level of Kelch-like ECH-associated protein 1 (Keap1). Taken together, our data demonstrated that the extraction of vine tea by 70% ethanol with ultrasonic treatment is a novel method to efficiently obtain components possessing stronger antioxidant activity. Furthermore, the results from the culture cells suggest that the bioactive component of vine tea might exert the antioxidant activity by activating the cellular Nrf2/Keap1 pathway. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Zhang, Haihan;Li, Dongfeng;Liu, Lingbin;Xu, Ling;Zhu, Mo;...

期刊： ANIMALS,2019年9(11) ISSN：2076-2615

通讯作者： He, Xi;Liu, Yang

作者机构： [He, Xi; Xu, Ling; Zhang, Haihan] Hunan Agr Univ, Dept Anim Sci, Changsha 410128, Hunan, Peoples R China.;[Zhu, Mo; Li, Dongfeng; Liu, Yang; Zhang, Haihan] Nanjing Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci & Technol, Natl Expt Teaching Demonstrat Ctr Anim Sci, Nanjing 210095, Jiangsu, Peoples R China.;[Zhang, Haihan] Indiana Univ Sch Med, Med Sci, Bloomington, IN 47408 USA.;[Liu, Lingbin] Southwest Univ, Coll Anim Sci & Technol, Chongqing 400715, Peoples R China.

通讯机构： [He, Xi] H;[Liu, Yang] N;Hunan Agr Univ, Dept Anim Sci, Changsha 410128, Hunan, Peoples R China.;Nanjing Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci & Technol, Natl Expt Teaching Demonstrat Ctr Anim Sci, Nanjing 210095, Jiangsu, Peoples R China.

关键词： chicken;differentiation;epithelium;small intestine

摘要： The small intestine plays an important role for animals to digest and absorb nutrients. The epithelial lining of the intestine develops from the embryonic endoderm of the embryo. The mature intestinal epithelium is composed of different types of functional epithelial cells that are derived from stem cells, which are located in the crypts. Chickens have been widely used as an animal model for researching vertebrate embryonic development. However, little is known about the molecular basis of development and differentiation within the chicken small intestinal epithelium. This review introduces processes of development and growth in the chicken gut, and compares the cellular characteristics and signaling pathways between chicken and mammals, including Notch and Wnt signaling that control the differentiation in the small intestinal epithelium. There is evidence that the chicken intestinal epithelium has a distinct cellular architecture and proliferation zone compared to mammals. The establishment of an in vitro cell culture model for chickens will provide a novel tool to explore molecular regulation of the chicken intestinal development and differentiation. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

语种： 英文

作者： Gu, X. L.;Li, H.;Song, Z. H.*;Ding, Y. N.;He, X.;...

期刊： Animal Reproduction Science,2019年207:52-60 ISSN：0378-4320

通讯作者： Song, Z. H.;Fan, Z. Y.

作者机构： [Ding, Y. N.; Fan, Z. Y.; Gu, X. L.; He, X.; Li, H.; Song, Z. H.] Hunan Agr Univ, Inst Anim Nutr, Minist Educ, Engn Res Ctr Feed Safety & Efficient Utilizat, Changsha 410128, Hunan, Peoples R China.;[Ding, Y. N.; Fan, Z. Y.; Gu, X. L.; He, X.; Li, H.; Song, Z. H.] Hunan Agr Univ, Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.;[Song, ZH; Fan, ZY] Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Song, ZH; Fan, ZY] H;Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China.

关键词： :Isomaltooligosaccharide;Bacillus;Placental antioxidation;Reproduction performance;Sows;Umbilical venous serum

摘要： This study investigated the effects of isomaltooligosaccharide (IMO) and Bacillus supplementation on sow performance, serum metabolites, and serum and placental oxidative status. Multiparous gestating sows (n = 130) with similar body conditions were randomly allocated to five groups (n = 26) receiving a basal diet (CON group) or a basal diet supplemented with 0.5% IMO (IMO group); 0.5% IMO and 0.02% Bacillus subtilis (IMO + S group); 0.5% IMO and 0.02% Bacillus licheniformis (IMO + L group); or 0.5% IMO, 0.02% Bacillus subtilis, and 0.02% Bacillus licheniformis (IMO + S+L group). There were no significant differences in the litter sizes among all dietary groups. The average piglet birth weight was improved in all treatment groups, and the placental efficiency was greater in the IMO + S and IMO + S+L groups than in the CON group (P < 0.05). The IMO + S+L group had increased the low-density lipoprotein cholesterol and reduced the total cholesterol in umbilical venous serum (P < 0.05). Additionally, the malondialdehyde concentrations were greater in umbilical venous serum of piglets in all treatment groups relative to that in the CON piglets (P < 0.05). The placental total antioxidant capacity was increased in the IMO+L and IMO+S+L groups (P < 0.05). Furthermore, the growth hormone concentration in umbilical venous serum was greater (P < 0.05) in all treatment groups. Overall, IMO and Bacillus supplementation during late gestation resulted in a changed metabolism of sows, improved the placental antioxidant capacity, and increased the growth hormone concentrations in umbilical venous serum, which ultimately improved the piglet birth weight and placental efficiency. © 2019 Elsevier B.V.

语种： 英文

作者： Li, R.;Hou, G. F.;Song, Z. H.*;Zhao, J. F.;Fan, Z. Y.;...

期刊： Animal Feed Science and Technology,2019年252:23-33 ISSN：0377-8401

通讯作者： Song, Z. H.;He, X.

作者机构： [Li, R.; Fan, Z. Y.; Hou, D-X.; Zhao, J. F.; Hou, G. F.; He, X.; Song, Z. H.; Song, ZH] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.;[Li, R.; Fan, Z. Y.; Zhao, J. F.; Hou, G. F.; He, X.; Song, Z. H.] Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China.;[Hou, D-X.] Kagoshima Univ, Fac Agr, Dept Food Sci & Biotechnol, Kagoshima 8900065, Japan.

通讯机构： [Song, ZH; He, X] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： Amino acid;Digestibility;Energy;Piglet;Protein sources

摘要： The digestible energy (DE) and metabolizable energy (ME) content (Exp.1), and apparent or standardized ileal digestibility coefficients (AIDC or SIDC) of crude protein (CP) and amino acid (AA) (Exp.2) in Soyppt-50% (S50), Hamlet Protein 300, (HP300), concentrated degossypolized cottonseed protein (CDCP), Palbio 50 RD (P50) and fish meal fed to piglets were estimated. In Exp.1, 36 barrows (10.19 ± 1.35 kg BW) were randomly divided into 6 diets with 6 pigs per diet. A basal diet and 5 diets containing 800 g/kg basal diet and 200 g/kg of each test protein ingredient were prepared. In Exp.2, 38 barrows (12.61 ± 1.45 kg BW) were randomly allotted to 1 of 6 diets with 6 pigs except for N-free diet (n = 8). 5 diets containing each of test protein ingredient as the sole source of AA and a N-free diet were formulated. At the end of the trial, ileal digesta of each pig was collected using the slaughter method. Results indicated that S50 contained more DE (16.28 MJ/kg) (P < 0.05) than CDCP (15.79 MJ/kg) and P50 (15.16 MJ/kg), but less (P < 0.05) than HP300 (16.53 MJ/kg) and fish meal (16.59 MJ/kg). The ME content in CDCP (14.68 MJ/kg) was less (P < 0.05) than in S50 (14.47 MJ/kg), HP300 (15.77 MJ/kg) and fish meal (15.63 MJ/kg), but greater (P < 0.05) than in P50 (13.11 MJ/kg). P50 had lower (P < 0.05) AIDC or SIDC of CP and most indispensable AA compared with other protein ingredients. AIDC or SIDC of CP and most indispensable AA in HP300 and CDCP were no differences, but greater (P < 0.05) than in other protein ingredients. S50 and fish meal had similar AIDC or SIDC of CP and most indispensable AA. In summary, S50, HP300 and CDCP had promising potential to replace fish meal in piglet diet after adding appropriate amount of fat and crystalline AA. © 2019

语种： 英文

作者： 刘勇强;赵旭;贺喜;施寿荣

期刊： 中国家禽,2019年(11):41-46 ISSN：1004-6364

作者机构： 湖南农业大学动物科学技术学院饲料安全与高效利用教育部工程研究中心湖南家禽安全生产工程技术研究中心湖南畜禽安全生产协同创新中心;中国农业科学院家禽研究所

关键词： 黄羽肉鸡;评定方法;氨基酸;需要量

摘要： 文章综述了黄羽肉鸡氨基酸需要量的评定方法及其主要必需氨基酸需要量的研究进展,总结黄羽肉鸡对赖氨酸、蛋氨酸、色氨酸以及苏氨酸的需要量。由于黄羽肉鸡种类较多,品种之间生长阶段划分不同,根据已有研究将黄羽肉鸡的生长划分为三个阶段:赖氨酸需要量第一阶段（1～28日龄）0.90%～1.13%,第二阶段（29～56日龄）0.77%～1.10%,第三阶段（57日龄至上市）0.75%～0.86%;蛋氨酸三个阶段需要量分别为0.42%～0.45%、0.29%～0.42%、0.24%～0.35%;色氨酸三个阶段需要量分别为0.17%～0.21%、0.18%～0.22%、0.17%～0.19%;苏氨酸三个阶段需要量分别为0.71%～0.87%、0.67%～0.78%、0.64%～0.77%。

语种： 中文

作者： Peng, Si-Min;Lin, Qian;Dai, Qiu-Zhong;He, Xi;Jiang, Gui-Tao*

期刊： Mitochondrial DNA Part B-Resources,2019年4(1):139-140 ISSN：2380-2359

通讯作者： Jiang, Gui-Tao

作者机构： [Peng, Si-Min] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Lin, Qian; Jiang, Gui-Tao; Dai, Qiu-Zhong] Hunan Inst Anim & Vet Sci, Res Dept Anim Nutr & Poultry, Changsha 410131, Hunan, Peoples R China.;[Lin, Qian; Dai, Qiu-Zhong] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha, Hunan, Peoples R China.;[He, Xi] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China.

通讯机构： [Jiang, Gui-Tao] H;Hunan Inst Anim & Vet Sci, Res Dept Anim Nutr & Poultry, Changsha 410131, Hunan, Peoples R China.

关键词： Zhijiang duck;mitochondrial;phylogenetic analyses

摘要： Zhijiang duck (ZJ) is one of the famous indigenous breed of Hunan province in China. The complete mitochondrial genome sequence plays an important role in the accurate determination of phylogenetic relationships among metazoans. It is the first time that the complete mitochondrial genome sequence of the ZJ was reported. The total length of the mtDNA is 16,604 bp. It contains the typical structure, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and 1 non-coding control region (D-loop region). The overall composition of the mtDNA was estimated to be 29.22% for A, 22.20% for T, 32.82% for C, and 15.77% for G. Phylogenetic analyses using N-J computational algorithms showed that the analyzed 20 Anseriform species are divided into four major clades: Anatinae, Anserinae, Dendrocygninae, and Anseranatidae. In addition, our work confirmed that ZJ and Anas poecilorhyncha have a close genetic relationship with fellow tribal members Linwu duck and Youxian duck. Meanwhile, we also found that ZJ and A. poecilorhyncha have highly similar genetic relationship. This work will provides an important data set for the study in the genetic mechanism of duck in Hunan province.

语种： 英文

作者： 丁鹏;丁亚南;曾青华;李霞;宋泽和;...

期刊： 动物营养学报,2019年31(9):4303-4313 ISSN：1006-267X

作者机构： [丁亚南; 贺喜; 丁鹏; 范志勇; 宋泽和; 曾青华] 湖南农业大学动物科学技术学院,饲料安全与高效利用教育部工程研究中心,湖南畜禽安全生产协同创新中心,长沙 410128;[曾青华] 湖南楚沩香农业有限公司,长沙 410005;湖南省蚕桑科学研究所,长沙,410127;[李霞] 湖南省蚕桑科学研究所

关键词： 发酵饲料桑粉;宁乡花猪;抗氧化;肠道功能

摘要： 本试验旨在研究饲粮添加发酵饲料桑粉对宁乡花猪其血清和肝脏抗氧化性能、肠道组织形态和肠道菌群的影响。选用平均体重为30 kg左右的宁乡花猪90头,随机分为5个组,每组3个重复(栏),每个重复6头猪。对照组饲喂基础饲粮,Ⅰ、Ⅱ、Ⅲ和Ⅳ组分别在基础饲粮中添加9%、12%、15%饲料桑粉的全发酵料和9%饲料桑粉的未发酵料。试验分为2个阶段,中猪阶段(第1 ~ 50天)和大猪阶段(第51 ~ 75天) 。结果表明: 1)与对照组相比,Ⅲ组宁乡花猪中期血清总抗氧化能力( T-AOC)显著提高( P< 0.05);饲喂添加饲料桑粉组宁乡花猪肝脏丙二醛( MDA)含量有下降的趋势( P>0.05),且随着饲料桑粉添加量的提高,下降愈为明显。2)与对照组相比,所有试验组宁乡花猪空肠绒毛高度显著降低( P<0.05); Ⅱ、Ⅲ和Ⅳ组宁乡花猪空肠绒毛高度/隐窝深度( V /C)值显著降低( P<0.05);与Ⅳ组相比,Ⅰ组宁乡花猪十二指肠绒毛高度有升高的趋势( P>0.05),空肠和回肠V /C值显著提高( P<0.05) 。3)对宁乡花猪回肠微生物进行16S rDNA测序发现:所有样品一共得到496 489条Tags,平均每个样品35 463条,Tag平均长度为252 bp,共产生257个操作分类单位( OTU) 。全发酵料组宁乡花猪回肠菌群Alpha多样性指数相比未发酵料组,OTU和Chao指数有提高的趋势,但差异不显著( P>0.05);各组间香农( Shannon)指数和辛普森( Simpson)指数均无显著差异( P>0.05) 。由此可知,饲料桑粉可有效提高宁乡花猪机体抗氧化性能并改善其肠道微生态环境;对饲料桑粉进行发酵处理后饲喂宁乡花猪可有效降低饲料桑粉对宁乡花猪肠道的负面影响。

语种： 中文

作者： 宋鹏;曹申平;唐建洲;彭亮;罗文婕;...

期刊： 水生生物学报,2019年43(6):1147-1154 ISSN：1000-3207

通讯作者： He, X.;Liu, Z.

作者机构： [贺喜] College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China;[曹申平; 熊鼎; 刘臻; 彭亮; 罗文婕] Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Environmental Engineering, Changsha University, Changsha, 410022, China;[邓岳松] TRS Group Co., Ltd., Zhuzhou, 412000, China;[宋鹏; 唐建洲] College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China, Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Environmental Engineering, Changsha University, Changsha, 410022, China

通讯机构： [He, X.] C;[Liu, Z.] H;College of Animal Science and Technology, China;Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, China

关键词： 发酵芝麻粕;草鱼;蛋白替代;生长性能;肠道吸收

摘要： 实验以初重（99.98±0.69） g的草鱼（Ctenopharyngodon idellus）为研究对象,研究饲料中发酵芝麻粕替代菜粕蛋白对草鱼生长性能、肠道形态和微生物以及小肽转运相关基因表达的影响。添加0、5%、10%和15%发酵芝麻粕分别替代菜粕蛋白0、11.8%、23.5%和35.1%,配制4组等氮等脂的饲料,分别为对照组、实验1组、实验2组和实验3组。实验在室内循环水养殖系统中进行,每组3个重复,每一重复饲喂20尾鱼,每天饱食投喂2次,实验共持续45d。结果发现:各处理组间草鱼增重率、特定生长率和蛋白质效率均无显著差异,实验1组和2组略高于对照组（P>0.05）;组间饵料系数也无显著差异,实验1组和2组略低于对照组（P>0.05）。实验组草鱼肠绒毛高度均显著高于对照组（P<0.05）,而实验组隐窝深度小于对照组（P>0.05）,绒毛高度与隐窝深度的比值（V/C）在实验组也显著高于对照组（P<0.05）。随着发酵芝麻粕替代比例的增加,乳酸杆菌（Lactobacillus）和芽孢杆菌（Bacillus）占比显著上升（P<0.05）,而大肠杆菌（Escherichia coli）和气单胞菌（Aeromonas）占比显著下降。小肽转运相关基因方面,尾型同源盒基因2 （CDX2）、特异性蛋白1 （Sp1）和小肽转运蛋白1 （PepT1）基因mRNA相对表达量均随着发酵芝麻粕替代比例的增加呈现先显著上调后下调的趋势,且均在实验1组时达到最大值（P<0.05）。综合考量鱼体生长性能、肠道黏膜形态、菌群及小肽转运相关基因表达方面,草鱼饲料中发酵芝麻粕蛋白适宜替代菜粕蛋白的比例为11.8%—23.5%。

语种： 中文

作者： Gu, Xue L.;Song, Ze H.*;Li, Hao;Wu, Sarah;Wu, Shu S.;...

期刊： Journal of the Science of Food and Agriculture,2019年99(13):5646-5653 ISSN：0022-5142

通讯作者： Song, Ze H.;Fan, Zhi Y.

作者机构： [He, Xi; Gu, Xue L.; Wu, Shu S.; Li, Hao; Ding, Ya N.; Song, Ze H.; Fan, Zhi Y.] Hunan Agr Univ, Inst Anim Nutr, Minist Educ, Engn Res Ctr Feed Safety & Efficient Utilizat, Changsha, Hunan, Peoples R China.;[Yin, Yu L.; He, Xi; Gu, Xue L.; Wu, Shu S.; Li, Hao; Ding, Ya N.; Song, Ze H.; Fan, Zhi Y.] Hunan Agr Univ, Hunan Coinnovat Ctr Anim Prod Safety, Changsha, Hunan, Peoples R China.;[Wu, Sarah] Univ Idaho, Biol Engn, Moscow, ID 83843 USA.;[Yin, Yu L.] Chinese Acad Sci, Inst Subtrop Agr, Hunan Prov Engn Res Ctr Hlth Livestock, Key Lab Agroecol Proc Subtrop Reg, Beijing, Peoples R China.;[Song, ZH; Fan, Zhi Y.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

通讯机构： [Song, ZH; Fan, ZY] H;Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： Bacillus spp.;blood metabolites;isomaltooligosaccharide;lactational performance;milk composition;sows

摘要： Background In this study, we evaluated the effects of isomaltooligosaccharide (IMO) and Bacillus spp. in perinatal diets on lactational performance, blood metabolites, and milk composition of sows. Multiparous gestating sows (N = 130) with similar body conditions were fed diets without containing IMO (control, CON group), IMO (IMO group), IMO and Bacillus subtilis (IMO + S group), IMO and Bacillus licheniformis (IMO + L group), and IMO and both B. subtilis and B. licheniformis (IMO + S + L group). Results The average daily feed intake of lactating sows in the IMO + S group (P < 0.05) and the average litter gain and total milk yield in the IMO + S + L group (P < 0.01) were significantly higher than those of the sows in the other treatment groups. In addition, the serum alanine aminotransferase and alkaline phosphatase concentrations in all treatment groups were lower than those in the CON group (P < 0.05). Furthermore, the highest proportion of fat in colostrum (0.05 P < 0.1) and lowest proportion of lactose in milk were observed in the IMO + L group (P < 0.05). The concentrations of growth hormone and immunoglobulins A and G in milk were higher in sows supplemented with IMO, IMO + S, IMO + L, or IMO + S + L (P < 0.05). Conclusion IMO and Bacillus spp. supplementation during the perinatal period improved the sows' lactational performance by affecting their metabolism and milk quality. Sows in the IMO + S and IMO + S + L treatment groups exhibited the best performance. (c) 2019 Society of Chemical Industry

语种： 英文

作者： 阳田恬;吕宏伟;贺喜;范志勇;宋泽和

期刊： 国外畜牧学：猪与禽,2019年39(8):21-23 ISSN：1001-0769

作者机构： 湖南农业大学动物科学技术学院,湖南 长沙 410128;湖南省畜禽安全生产协同创新中心,湖南 长沙 410128;[贺喜; 范志勇; 宋泽和; 吕宏伟; 阳田恬] 湖南农业大学

关键词： 乳寡糖;生理功能;代谢;猪乳寡糖

摘要： 乳寡糖(Milk Oligosaccharides,MOs)是一类存在于母乳中的天然益生元,在母乳中为仅次于脂肪和乳糖的第三大固有成分.MOs本质为一系列结构复杂的糖分子,猪乳寡糖(Porcine Milk Oligosaccharides,PMOs)主要分为岩藻糖MOs(fucosylated-MOs,fuc-MOs)、中性MOs(neutral-MOs,neu-MOs)和唾液酸化MOs(sialylated-MOs,sia-MOs).MOs不仅可被动物肠道消化吸收,还可作为底物被肠道微生物利用.PMOs具有调节肠道菌群结构、调节免疫应答和抵抗有害微生物黏附的能力,因而在仔猪肠道菌群塑造和免疫构建过程中发挥着重要功能.本文阐述了PMOs的组成成分、MOs的生理功能及其代谢,旨在为PMOs应用于动物生产提供理论参考.

语种： 中文

作者： 曹蓉;贺喜;尹华;沈维军;贺长青;...

期刊： 科教文汇(下旬刊),2019年(30):70-72 ISSN：1672-7894

作者机构： 湖南农业大学动物科学技术学院 湖南·长沙 410128

关键词： 虚拟仿真实验教学;饲料加工工艺与设备;问卷调查

摘要： 虚拟仿真技术实验教学具有教学交互性强、教学效果深入、教学环境逼真和虚幻性等特点,在饲料加工工艺与设备实验教学中具有显著的优势。本研究基于对湖南农业大学动物科学专业的问卷调查得知:构建虚拟仿真实验教学模式有助于激发大学生对实验课程的兴趣,有效提升学生对基础知识的掌握程度,改善实践操作技能和自身的长远发展。

语种： 中文

作者： 冯淦熠;贺喜;杨浩然;项轩;黄慧;...

期刊： 饲料研究,2019年42(2):68-72 ISSN：1002-2813

作者机构： 湖南农业大学动物科学技术学院,湖南长沙,410128;[杨浩然; 曹蓉; 贺喜; 项轩; 黄慧; 李新化; 冯淦熠] 湖南农业大学

关键词： 桑叶;多酚类物质;提取方法;抗氧化能力

摘要： 我国是传统的种桑养蚕大国,桑树广泛分布于全国各地,桑叶资源十分丰富。近年来,桑叶逐渐应用于畜禽生产中,具有提高动物生产性能及饲料转化率、改善动物健康状态等作用。但关于桑叶抗氧化活性成分的提取及其体外抗氧化能力的研究较少,不利于进一步开发利用宝贵的桑叶资源。文章通过对桑叶营养成分和生理功能、多酚类物质的提取方法和体外抗氧化作用等相关研究进行了综述,为开展桑叶体外抗氧化能力测定试验提供一定的技术指导。

语种： 中文